TY  - JOUR
AU  - Čkrkić, Jelisaveta
AU  - Petrovic, Andjelko
AU  - Kocić, Korana
AU  - Mitrović, Milana
AU  - Kavallieratos, Nickolas
AU  - Van Achterberg, Cornelis
AU  - Hebert, Paul
AU  - Tomanović, Željko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/590
AB  - Abstract: Members of the Monoctonina subtribe have long been neglected in applied studies of
the subfamily Aphidiinae, due to their low economic importance, as they do not parasitize pests
of cultivated plants. Consequently, data about this group are scarce, including its taxonomy and
phylogeny. In the present study, we explore inter- and intraspecific genetic variation of Monoctonina
species, including genera Monoctonus Haliday 1833, Monoctonia Starý 1962, Falciconus Mackauer
1959 and Harkeria Cameron 1900. We employ two molecular markers, the barcode region of the
mitochondrial cytochrome c oxidase subunit I (COI) and the D2 region of the 28S nuclear gene
(28S rDNA), to analyze genetic structuring and phylogeny of all available Monoctonina species,
and combine them with morphological data for an integrative approach. We report one new species,
and three potentially new species which can be formally described when further specimens are
available. Analysis of phylogenetic relationships within the subtribe shows a basal position for the
genera Falciconus and Monoctonia, and the close relatedness of Harkeria and Monoctonus.
PB  - Basel : MDPI
T2  - Insects
T1  - Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae)
IS  - 160
IS  - 3
VL  - 11
DO  - 10.3390/insects11030160
ER  - 

@article{
author = "Čkrkić, Jelisaveta and Petrovic, Andjelko and Kocić, Korana and Mitrović, Milana and Kavallieratos, Nickolas and Van Achterberg, Cornelis and Hebert, Paul and Tomanović, Željko",
year = "2020",
abstract = "Abstract: Members of the Monoctonina subtribe have long been neglected in applied studies of
the subfamily Aphidiinae, due to their low economic importance, as they do not parasitize pests
of cultivated plants. Consequently, data about this group are scarce, including its taxonomy and
phylogeny. In the present study, we explore inter- and intraspecific genetic variation of Monoctonina
species, including genera Monoctonus Haliday 1833, Monoctonia Starý 1962, Falciconus Mackauer
1959 and Harkeria Cameron 1900. We employ two molecular markers, the barcode region of the
mitochondrial cytochrome c oxidase subunit I (COI) and the D2 region of the 28S nuclear gene
(28S rDNA), to analyze genetic structuring and phylogeny of all available Monoctonina species,
and combine them with morphological data for an integrative approach. We report one new species,
and three potentially new species which can be formally described when further specimens are
available. Analysis of phylogenetic relationships within the subtribe shows a basal position for the
genera Falciconus and Monoctonia, and the close relatedness of Harkeria and Monoctonus.",
publisher = "Basel : MDPI",
journal = "Insects",
title = "Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae)",
number = "160, 3",
volume = "11",
doi = "10.3390/insects11030160"
}

Čkrkić, J., Petrovic, A., Kocić, K., Mitrović, M., Kavallieratos, N., Van Achterberg, C., Hebert, P.,& Tomanović, Ž.. (2020). Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae). in Insects
Basel : MDPI., 11(160).
https://doi.org/10.3390/insects11030160

Čkrkić J, Petrovic A, Kocić K, Mitrović M, Kavallieratos N, Van Achterberg C, Hebert P, Tomanović Ž. Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae). in Insects. 2020;11(160).
doi:10.3390/insects11030160 .

Čkrkić, Jelisaveta, Petrovic, Andjelko, Kocić, Korana, Mitrović, Milana, Kavallieratos, Nickolas, Van Achterberg, Cornelis, Hebert, Paul, Tomanović, Željko, "Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae)" in Insects, 11, no. 160 (2020),
https://doi.org/10.3390/insects11030160 . .

TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/588
AB  - Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.
PB  - Springer Nature
T2  - European Journal of Plant Pathology
T1  - Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential
EP  - 221
SP  - 201
VL  - 156
DO  - 10.1007/s10658-019-01878-w
ER  - 

@article{
author = "Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Mitrović, Milana and Marinković, Slavica and Toševski, Ivo and Cvrković, Tatjana",
year = "2020",
abstract = "Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.",
publisher = "Springer Nature",
journal = "European Journal of Plant Pathology",
title = "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential",
pages = "221-201",
volume = "156",
doi = "10.1007/s10658-019-01878-w"
}

Jakovljević, M., Jović, J., Krstić, O., Mitrović, M., Marinković, S., Toševski, I.,& Cvrković, T.. (2020). Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology
Springer Nature., 156, 201-221.
https://doi.org/10.1007/s10658-019-01878-w

Jakovljević M, Jović J, Krstić O, Mitrović M, Marinković S, Toševski I, Cvrković T. Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology. 2020;156:201-221.
doi:10.1007/s10658-019-01878-w .

Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Mitrović, Milana, Marinković, Slavica, Toševski, Ivo, Cvrković, Tatjana, "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential" in European Journal of Plant Pathology, 156 (2020):201-221,
https://doi.org/10.1007/s10658-019-01878-w . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Stary, Etr
AU  - Jakovljević, Miljana
AU  - Petrović, Anđeljko
AU  - Zikić, Vladimir
AU  - Perez Hidalgo, Nicolas
AU  - Tomanović, Željko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/571
AB  - Species from the genus Paralipsis are obligatory endoparasitoids of root aphids in the Palaearctic. It is known that these species are broadly distributed, parasitizing various aphid hosts and showing great biological and ecological diversity. On the other hand, this group of endoparasitoids is understudied and was thought to be represented by a single species in Europe, viz., Paralipsis enervis (Nees). However, recent description of two new species indicated the possibility of cryptic speciation and recognition of additional Paralipsis species in Europe. In this research, Paralipsis specimens collected during the last 60 years from eight European countries, as well as one sample from Morocco, were subjected to molecular and morphological characterization. Newly designed genus-specific degenerative primers successfully targeted short overlapping fragments of COI of the mitochondrial DNA. Molecular analyses showed clear separation of four independent lineages, two of which are the known species P. enervis and P. tibiator, while two new species are described here, viz., P. brachycaudi Tomanovic & Stary, sp. n. and P. rugosa Tomanovic & Stary, sp. n. No clear specialization of the taxa to a strict root aphid host has been determined. The recognized mitochondrial lineages were distinct one from another, but with a substantial within-lineage divergence rate, clearly indicating the complexity of this group of parasitoids, on which further research is required in order to clarify the factors triggering their genetic differentiation. We reviewed literature data and new records of Paralipsis enervis aphid host associations and distributions. A key for the identification of all known Paralipsis species is provided and illustrated.
PB  - Pensoft Publishers
T2  - Zookeys
T1  - Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species
EP  - 69
IS  - 831
SP  - 49
DO  - 10.3897/zookeys.831.31808
ER  - 

@article{
author = "Mitrović, Milana and Stary, Etr and Jakovljević, Miljana and Petrović, Anđeljko and Zikić, Vladimir and Perez Hidalgo, Nicolas and Tomanović, Željko",
year = "2019",
abstract = "Species from the genus Paralipsis are obligatory endoparasitoids of root aphids in the Palaearctic. It is known that these species are broadly distributed, parasitizing various aphid hosts and showing great biological and ecological diversity. On the other hand, this group of endoparasitoids is understudied and was thought to be represented by a single species in Europe, viz., Paralipsis enervis (Nees). However, recent description of two new species indicated the possibility of cryptic speciation and recognition of additional Paralipsis species in Europe. In this research, Paralipsis specimens collected during the last 60 years from eight European countries, as well as one sample from Morocco, were subjected to molecular and morphological characterization. Newly designed genus-specific degenerative primers successfully targeted short overlapping fragments of COI of the mitochondrial DNA. Molecular analyses showed clear separation of four independent lineages, two of which are the known species P. enervis and P. tibiator, while two new species are described here, viz., P. brachycaudi Tomanovic & Stary, sp. n. and P. rugosa Tomanovic & Stary, sp. n. No clear specialization of the taxa to a strict root aphid host has been determined. The recognized mitochondrial lineages were distinct one from another, but with a substantial within-lineage divergence rate, clearly indicating the complexity of this group of parasitoids, on which further research is required in order to clarify the factors triggering their genetic differentiation. We reviewed literature data and new records of Paralipsis enervis aphid host associations and distributions. A key for the identification of all known Paralipsis species is provided and illustrated.",
publisher = "Pensoft Publishers",
journal = "Zookeys",
title = "Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species",
pages = "69-49",
number = "831",
doi = "10.3897/zookeys.831.31808"
}

Mitrović, M., Stary, E., Jakovljević, M., Petrović, A., Zikić, V., Perez Hidalgo, N.,& Tomanović, Ž.. (2019). Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species. in Zookeys
Pensoft Publishers.(831), 49-69.
https://doi.org/10.3897/zookeys.831.31808

Mitrović M, Stary E, Jakovljević M, Petrović A, Zikić V, Perez Hidalgo N, Tomanović Ž. Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species. in Zookeys. 2019;(831):49-69.
doi:10.3897/zookeys.831.31808 .

Mitrović, Milana, Stary, Etr, Jakovljević, Miljana, Petrović, Anđeljko, Zikić, Vladimir, Perez Hidalgo, Nicolas, Tomanović, Željko, "Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species" in Zookeys, no. 831 (2019):49-69,
https://doi.org/10.3897/zookeys.831.31808 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/565
AB  - Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia
EP  - 195
IS  - 1
SP  - 183
VL  - 153
DO  - 10.1007/s10658-018-1553-1
ER  - 

@article{
author = "Kosovac, Andrea and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2019",
abstract = "Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia",
pages = "195-183",
number = "1",
volume = "153",
doi = "10.1007/s10658-018-1553-1"
}

Kosovac, A., Jakovljević, M., Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2019). Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 153(1), 183-195.
https://doi.org/10.1007/s10658-018-1553-1

Kosovac A, Jakovljević M, Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology. 2019;153(1):183-195.
doi:10.1007/s10658-018-1553-1 .

Kosovac, Andrea, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia" in European Journal of Plant Pathology, 153, no. 1 (2019):183-195,
https://doi.org/10.1007/s10658-018-1553-1 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/547
AB  - The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).
PB  - Public Library of Science (PLoS)
T2  - PlOS One
T1  - Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation
IS  - 5
VL  - 13
DO  - 10.1371/journal.pone.0196969
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).",
publisher = "Public Library of Science (PLoS)",
journal = "PlOS One",
title = "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation",
number = "5",
volume = "13",
doi = "10.1371/journal.pone.0196969"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2018). Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One
Public Library of Science (PLoS)., 13(5).
https://doi.org/10.1371/journal.pone.0196969

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One. 2018;13(5).
doi:10.1371/journal.pone.0196969 .

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation" in PlOS One, 13, no. 5 (2018),
https://doi.org/10.1371/journal.pone.0196969 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Tomanović, Željko
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/559
AB  - Archival specimens are a great resource for molecular research in population biology, taxonomy and conservation. A primary goal for researchers is to preserve specimens from collections by improving noninvasive methods for DNA extraction and to achieve successful amplification of the short fragments of a target gene in the event of DNA fragmentation. We tested the suitability of a noninvasive method of DNA extraction and amplification of the barcoding region of the mitochondrial gene cytochrome c oxidise subunit I from archival specimens of aphid parasitoids belonging to the genera Aphidius, Lysiphlebus and Praon (Aphidiinae, Braconidae, Hymenoptera). Using a commercial kit as a noninvasive method, we successfully extracted DNA from dry 7 to 41 year old samples of 26 different parasitoid species. However, amplification of the barcoding region failed using the standard primer pair LCO1490/HCO2198. In order to reconstruct DNA barcodes we designed internal genus-specific degenerative primers and a new amplification protocol to target the short fragments within the mitochondrial region. Novel primers were designed using as a template the reference sequences from congeners retrieved from the public database. The combination of standard primers with internal primers, in direct and nested amplification reactions, produced short overlapping subsequences, concatenated to recover long barcoding sequences. Additional analyses also confirmed that primers initially designed for Aphidius, Lysiphk.hus and Praon can be combined in a mixture, and successfully used to obtain short fragments of disintegrated DNA from archival specimens of several other braconid species from the genera Ephedrus and Monoctonus.
PB  - Pensoft Publishers
T2  - Journal of Hymenoptera Research
T1  - New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)
EP  - 210
SP  - 191
VL  - 64
DO  - 10.3897/jhr.64.25399
ER  - 

@article{
author = "Mitrović, Milana and Tomanović, Željko",
year = "2018",
abstract = "Archival specimens are a great resource for molecular research in population biology, taxonomy and conservation. A primary goal for researchers is to preserve specimens from collections by improving noninvasive methods for DNA extraction and to achieve successful amplification of the short fragments of a target gene in the event of DNA fragmentation. We tested the suitability of a noninvasive method of DNA extraction and amplification of the barcoding region of the mitochondrial gene cytochrome c oxidise subunit I from archival specimens of aphid parasitoids belonging to the genera Aphidius, Lysiphlebus and Praon (Aphidiinae, Braconidae, Hymenoptera). Using a commercial kit as a noninvasive method, we successfully extracted DNA from dry 7 to 41 year old samples of 26 different parasitoid species. However, amplification of the barcoding region failed using the standard primer pair LCO1490/HCO2198. In order to reconstruct DNA barcodes we designed internal genus-specific degenerative primers and a new amplification protocol to target the short fragments within the mitochondrial region. Novel primers were designed using as a template the reference sequences from congeners retrieved from the public database. The combination of standard primers with internal primers, in direct and nested amplification reactions, produced short overlapping subsequences, concatenated to recover long barcoding sequences. Additional analyses also confirmed that primers initially designed for Aphidius, Lysiphk.hus and Praon can be combined in a mixture, and successfully used to obtain short fragments of disintegrated DNA from archival specimens of several other braconid species from the genera Ephedrus and Monoctonus.",
publisher = "Pensoft Publishers",
journal = "Journal of Hymenoptera Research",
title = "New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)",
pages = "210-191",
volume = "64",
doi = "10.3897/jhr.64.25399"
}

Mitrović, M.,& Tomanović, Ž.. (2018). New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae). in Journal of Hymenoptera Research
Pensoft Publishers., 64, 191-210.
https://doi.org/10.3897/jhr.64.25399

Mitrović M, Tomanović Ž. New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae). in Journal of Hymenoptera Research. 2018;64:191-210.
doi:10.3897/jhr.64.25399 .

Mitrović, Milana, Tomanović, Željko, "New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)" in Journal of Hymenoptera Research, 64 (2018):191-210,
https://doi.org/10.3897/jhr.64.25399 . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/550
AB  - The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions
EP  - 64
IS  - 1
SP  - 47
VL  - 172
DO  - 10.1111/aab.12400
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Radonjić, Sanja and Hrnčić, Snježana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions",
pages = "64-47",
number = "1",
volume = "172",
doi = "10.1111/aab.12400"
}

Krstić, O., Cvrković, T., Mitrović, M., Radonjić, S., Hrnčić, S., Toševski, I.,& Jović, J.. (2018). Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology
Wiley, Hoboken., 172(1), 47-64.
https://doi.org/10.1111/aab.12400

Krstić O, Cvrković T, Mitrović M, Radonjić S, Hrnčić S, Toševski I, Jović J. Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology. 2018;172(1):47-64.
doi:10.1111/aab.12400 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Radonjić, Sanja, Hrnčić, Snježana, Toševski, Ivo, Jović, Jelena, "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions" in Annals of Applied Biology, 172, no. 1 (2018):47-64,
https://doi.org/10.1111/aab.12400 . .

TY  - JOUR
AU  - Petrović, A.
AU  - Mitrović, Milana
AU  - Ghaliow, M.E.
AU  - Ivanović, Ana
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Tomanović, Željko
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/527
AB  - Species that belong to the Aphidius eadyi group have been used as biocontrol agents against Acyrthosiphon pisum worldwide. However, despite their extensive use, there are still gaps in our knowledge about their taxonomy and distribution. In this study, we employed an integrative taxonomic approach by combining genetic analyses (mtDNA COI barcoding) with standard morphological analyses and geometric morphometrics of forewing shape. We identified three species within the A. eadyi species group, viz., A. smithi, A. eadyi and A. banksae. Genetic separation of all three species was confirmed, with mean genetic distances between species ranging from 5 to 7.4%. The following morphological characters were determined as the most important for separating species of the A. eadyi group: number and shape of costulae on the anterolateral part of the petiole, shape of the central areola on the propodeum, and shape and venation of the forewings. The differences in wing shape of all three species were statistically significant, but with some overlapping. We identified A. banksae as a widely distributed pea aphid parasitoid, whose known range covers most of the western Palaearctic (from the UK to Israel). Aphidius banksae is diagnosed and redescribed.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach
EP  - 14
SP  - 1
DO  - 10.1017/S000748531800055X
ER  - 

@article{
author = "Petrović, A. and Mitrović, Milana and Ghaliow, M.E. and Ivanović, Ana and Kavallieratos, Nickolas G. and Stary, Petr and Tomanović, Željko",
year = "2018",
abstract = "Species that belong to the Aphidius eadyi group have been used as biocontrol agents against Acyrthosiphon pisum worldwide. However, despite their extensive use, there are still gaps in our knowledge about their taxonomy and distribution. In this study, we employed an integrative taxonomic approach by combining genetic analyses (mtDNA COI barcoding) with standard morphological analyses and geometric morphometrics of forewing shape. We identified three species within the A. eadyi species group, viz., A. smithi, A. eadyi and A. banksae. Genetic separation of all three species was confirmed, with mean genetic distances between species ranging from 5 to 7.4%. The following morphological characters were determined as the most important for separating species of the A. eadyi group: number and shape of costulae on the anterolateral part of the petiole, shape of the central areola on the propodeum, and shape and venation of the forewings. The differences in wing shape of all three species were statistically significant, but with some overlapping. We identified A. banksae as a widely distributed pea aphid parasitoid, whose known range covers most of the western Palaearctic (from the UK to Israel). Aphidius banksae is diagnosed and redescribed.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach",
pages = "14-1",
doi = "10.1017/S000748531800055X"
}

Petrović, A., Mitrović, M., Ghaliow, M.E., Ivanović, A., Kavallieratos, N. G., Stary, P.,& Tomanović, Ž.. (2018). Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 1-14.
https://doi.org/10.1017/S000748531800055X

Petrović A, Mitrović M, Ghaliow M, Ivanović A, Kavallieratos NG, Stary P, Tomanović Ž. Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach. in Bulletin of Entomological Research. 2018;:1-14.
doi:10.1017/S000748531800055X .

Petrović, A., Mitrović, Milana, Ghaliow, M.E., Ivanović, Ana, Kavallieratos, Nickolas G., Stary, Petr, Tomanović, Željko, "Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach" in Bulletin of Entomological Research (2018):1-14,
https://doi.org/10.1017/S000748531800055X . .

TY  - JOUR
AU  - Tomanović, Željko
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Kavallieratos, Nickolas G.
AU  - Zikić, Vladimir
AU  - Ivanović, Ana
AU  - Rakhshani, Ehsan
AU  - Stary, Petr
AU  - Vorburger, Christoph
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/519
AB  - Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributed worldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus species are revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits, and on information about their host specificity. The taxonomic status of 13 European Lysiphlebus species is confirmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall, 1896)], L. hirtus Stark, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safavii Stary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Our results, based on both COI and 28SD2, confirm the existence of three monophyletic species groups within the genus: (1) the 'fabarum' group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2) the 'testaceipes' group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer, 1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the 'alpinus' group, consisting solely of L. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the fabarum' group. We were able to confirm for the first time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentially asexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key for identification of the European Lysiphlebus species is provided.
PB  - Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden
T2  - Arthropod Systematics & Phylogeny
T1  - Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology
EP  - 213
IS  - 2
SP  - 179
VL  - 76
ER  - 

@article{
author = "Tomanović, Željko and Mitrović, Milana and Petrović, Anđeljko and Kavallieratos, Nickolas G. and Zikić, Vladimir and Ivanović, Ana and Rakhshani, Ehsan and Stary, Petr and Vorburger, Christoph",
year = "2018",
abstract = "Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributed worldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus species are revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits, and on information about their host specificity. The taxonomic status of 13 European Lysiphlebus species is confirmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall, 1896)], L. hirtus Stark, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safavii Stary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Our results, based on both COI and 28SD2, confirm the existence of three monophyletic species groups within the genus: (1) the 'fabarum' group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2) the 'testaceipes' group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer, 1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the 'alpinus' group, consisting solely of L. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the fabarum' group. We were able to confirm for the first time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentially asexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key for identification of the European Lysiphlebus species is provided.",
publisher = "Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden",
journal = "Arthropod Systematics & Phylogeny",
title = "Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology",
pages = "213-179",
number = "2",
volume = "76"
}

Tomanović, Ž., Mitrović, M., Petrović, A., Kavallieratos, N. G., Zikić, V., Ivanović, A., Rakhshani, E., Stary, P.,& Vorburger, C.. (2018). Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology. in Arthropod Systematics & Phylogeny
Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden., 76(2), 179-213.

Tomanović Ž, Mitrović M, Petrović A, Kavallieratos NG, Zikić V, Ivanović A, Rakhshani E, Stary P, Vorburger C. Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology. in Arthropod Systematics & Phylogeny. 2018;76(2):179-213..

Tomanović, Željko, Mitrović, Milana, Petrović, Anđeljko, Kavallieratos, Nickolas G., Zikić, Vladimir, Ivanović, Ana, Rakhshani, Ehsan, Stary, Petr, Vorburger, Christoph, "Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology" in Arthropod Systematics & Phylogeny, 76, no. 2 (2018):179-213.

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Ckrkić, Jelisaveta
AU  - Jamhour, Aiman
AU  - Petrović-Obradović, Olivera
AU  - Mitrović, Milana
AU  - Stary, Petr
AU  - Nedstam, Barbro
AU  - Tomanović, Željko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/483
AB  - Aphidius ericaphidis, an aphid parasitoid originally described from North America, is recorded in Europe for the first time, based on morphological and molecular analyses. The species is recorded in Serbia, the Netherlands, Sweden and the United Kingdom. It was formerly recorded as Aphidius sp./Ericaphis latifrons/Vaccinium uliginosum from the Alps (France). The possible origin of A. ericaphidis, as well as its likely distribution, is discussed in relation to its host. As it is a specific parasitoid of Ericaphis aphids, especially the invasive aphid Ericaphis scammelli on blueberries, its potential as a biocontrol agent is also discussed.
PB  - Pensoft Publishers
T2  - Journal of Hymenoptera Research
T1  - First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?
EP  - 153
SP  - 143
VL  - 57
DO  - 10.3897/jhr.57.12517
ER  - 

@article{
author = "Petrović, Anđeljko and Ckrkić, Jelisaveta and Jamhour, Aiman and Petrović-Obradović, Olivera and Mitrović, Milana and Stary, Petr and Nedstam, Barbro and Tomanović, Željko",
year = "2017",
abstract = "Aphidius ericaphidis, an aphid parasitoid originally described from North America, is recorded in Europe for the first time, based on morphological and molecular analyses. The species is recorded in Serbia, the Netherlands, Sweden and the United Kingdom. It was formerly recorded as Aphidius sp./Ericaphis latifrons/Vaccinium uliginosum from the Alps (France). The possible origin of A. ericaphidis, as well as its likely distribution, is discussed in relation to its host. As it is a specific parasitoid of Ericaphis aphids, especially the invasive aphid Ericaphis scammelli on blueberries, its potential as a biocontrol agent is also discussed.",
publisher = "Pensoft Publishers",
journal = "Journal of Hymenoptera Research",
title = "First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?",
pages = "153-143",
volume = "57",
doi = "10.3897/jhr.57.12517"
}

Petrović, A., Ckrkić, J., Jamhour, A., Petrović-Obradović, O., Mitrović, M., Stary, P., Nedstam, B.,& Tomanović, Ž.. (2017). First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?. in Journal of Hymenoptera Research
Pensoft Publishers., 57, 143-153.
https://doi.org/10.3897/jhr.57.12517

Petrović A, Ckrkić J, Jamhour A, Petrović-Obradović O, Mitrović M, Stary P, Nedstam B, Tomanović Ž. First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?. in Journal of Hymenoptera Research. 2017;57:143-153.
doi:10.3897/jhr.57.12517 .

Petrović, Anđeljko, Ckrkić, Jelisaveta, Jamhour, Aiman, Petrović-Obradović, Olivera, Mitrović, Milana, Stary, Petr, Nedstam, Barbro, Tomanović, Željko, "First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?" in Journal of Hymenoptera Research, 57 (2017):143-153,
https://doi.org/10.3897/jhr.57.12517 . .

TY  - JOUR
AU  - Trivellone, Valeria
AU  - Mitrović, Milana
AU  - Dietrich, Christopher H.
AU  - Toševski, Ivo
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/477
AB  - The introduction of invasive species may result in important ecological, environmental, and economic impacts. Extensive study of Auchenorrhyncha (Hemiptera) fauna in a wine-growing region in southern Switzerland revealed, for the first time, presence of the Nearctic leafhopper Osbornellus auronitens (Provancher) (Hemiptera: Cicadellidae) in the vicinity of Stabio (Canton of Ticino). The species identity of the collected specimens was confirmed using morphological and molecular characters. All specimens of O. auronitens were collected in a forest of Castanea sativa Miller (Fagaceae), Corylus avellana Linnaeus (Betulaceae), and Alnus glutinosa (Linnaeus) Gaertner (Betulaceae) intermixed with Cornus sanguinea Linnaeus (Cornaceae), Salix Linnaeus (Salicaceae), and Rubus Linnaeus (Rosaceae). In its native range this leafhopper is polyphagous and a relatively common visitor in vineyards. Based on analysis of the barcoding region of the mitochondrial cytochrome oxidase subunit I gene of the collected O. auronitens, 100% identity with specimens of the same species originating from Canada was determined. Osbornellus auronitens is morphologically similar to Scaphoideus titanus Ball (Hemiptera: Cicadellidae), another introduced Nearctic leafhopper, which was involved in severe outbreaks of disease caused by the Grapevine flavescence doree phytoplasma (Bacteria: Acholeplasmataceae) in European viticultural regions since the 1960s. In this paper, we report the morphological features to distinguish O. auronitens from S. titanus, and discuss the possible implications of its expected spread across the Old World.
PB  - Cambridge Univ Press, New York
T2  - Canadian Entomologist
T1  - Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region
EP  - 559
IS  - 5
SP  - 551
VL  - 149
DO  - 10.4039/tce.2017.7
ER  - 

@article{
author = "Trivellone, Valeria and Mitrović, Milana and Dietrich, Christopher H. and Toševski, Ivo",
year = "2017",
abstract = "The introduction of invasive species may result in important ecological, environmental, and economic impacts. Extensive study of Auchenorrhyncha (Hemiptera) fauna in a wine-growing region in southern Switzerland revealed, for the first time, presence of the Nearctic leafhopper Osbornellus auronitens (Provancher) (Hemiptera: Cicadellidae) in the vicinity of Stabio (Canton of Ticino). The species identity of the collected specimens was confirmed using morphological and molecular characters. All specimens of O. auronitens were collected in a forest of Castanea sativa Miller (Fagaceae), Corylus avellana Linnaeus (Betulaceae), and Alnus glutinosa (Linnaeus) Gaertner (Betulaceae) intermixed with Cornus sanguinea Linnaeus (Cornaceae), Salix Linnaeus (Salicaceae), and Rubus Linnaeus (Rosaceae). In its native range this leafhopper is polyphagous and a relatively common visitor in vineyards. Based on analysis of the barcoding region of the mitochondrial cytochrome oxidase subunit I gene of the collected O. auronitens, 100% identity with specimens of the same species originating from Canada was determined. Osbornellus auronitens is morphologically similar to Scaphoideus titanus Ball (Hemiptera: Cicadellidae), another introduced Nearctic leafhopper, which was involved in severe outbreaks of disease caused by the Grapevine flavescence doree phytoplasma (Bacteria: Acholeplasmataceae) in European viticultural regions since the 1960s. In this paper, we report the morphological features to distinguish O. auronitens from S. titanus, and discuss the possible implications of its expected spread across the Old World.",
publisher = "Cambridge Univ Press, New York",
journal = "Canadian Entomologist",
title = "Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region",
pages = "559-551",
number = "5",
volume = "149",
doi = "10.4039/tce.2017.7"
}

Trivellone, V., Mitrović, M., Dietrich, C. H.,& Toševski, I.. (2017). Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region. in Canadian Entomologist
Cambridge Univ Press, New York., 149(5), 551-559.
https://doi.org/10.4039/tce.2017.7

Trivellone V, Mitrović M, Dietrich CH, Toševski I. Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region. in Canadian Entomologist. 2017;149(5):551-559.
doi:10.4039/tce.2017.7 .

Trivellone, Valeria, Mitrović, Milana, Dietrich, Christopher H., Toševski, Ivo, "Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region" in Canadian Entomologist, 149, no. 5 (2017):551-559,
https://doi.org/10.4039/tce.2017.7 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Khan, Mohamed F. R.
AU  - Secor, Gary A.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/475
AB  - Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides
EP  - 910
IS  - 4
SP  - 895
VL  - 149
DO  - 10.1007/s10658-017-1239-0
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Khan, Mohamed F. R. and Secor, Gary A.",
year = "2017",
abstract = "Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides",
pages = "910-895",
number = "4",
volume = "149",
doi = "10.1007/s10658-017-1239-0"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J., Toševski, I., Khan, M. F. R.,& Secor, G. A.. (2017). Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology
Springer, Dordrecht., 149(4), 895-910.
https://doi.org/10.1007/s10658-017-1239-0

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I, Khan MFR, Secor GA. Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology. 2017;149(4):895-910.
doi:10.1007/s10658-017-1239-0 .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Khan, Mohamed F. R., Secor, Gary A., "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides" in European Journal of Plant Pathology, 149, no. 4 (2017):895-910,
https://doi.org/10.1007/s10658-017-1239-0 . .

TY  - JOUR
AU  - Popović, Aleksandra
AU  - Mitrović, Milana
AU  - Petrović, Miloš
AU  - Petrović, Aleksandra
AU  - Bursić, Vojislava
AU  - Marinković, Dušan
AU  - Gvozdenac, Sonja
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/440
AB  - Based on the number of individuals and percentage of encounters in the studied areas, it is concluded that ground beetles (Coleoptera: Carabidae) stand out in comparison to other beetles, which is proved by the qualitative and quantitative composition of the collected fauna (51 species and 4,420 individuals) in the field of wheat, sugar beet and maize. Starting from the domination of geobiotic insects which spend most of their life in soil and manifest their imaginal activity on the surface of soil, the method of "Barber's traps" was applied. Within this family the dominant and subdominant species during research were Anchomenus dorsalis, Poecilus cupreus, Harpalus rufipes, Calosoma auropunctatum, Harpalu distinguendus and many others. 24 carabids species were determined using the COI mtDNA, i.e. 8 species which don't have reference sequence in the gene bank were determined and this contributes to the global bar-code database. The representatives of ground beetle family are of certain economic significance as well. They can appear as pests; on the other hand, they are known to be very beneficial i.e. as regulators of number of harmful insects as reported in numerous research papers of various authors.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Molecular Identification of Ground Beetles on Arable Land
EP  - 11364
IS  - 2
SP  - 11357
VL  - 21
ER  - 

@article{
author = "Popović, Aleksandra and Mitrović, Milana and Petrović, Miloš and Petrović, Aleksandra and Bursić, Vojislava and Marinković, Dušan and Gvozdenac, Sonja",
year = "2016",
abstract = "Based on the number of individuals and percentage of encounters in the studied areas, it is concluded that ground beetles (Coleoptera: Carabidae) stand out in comparison to other beetles, which is proved by the qualitative and quantitative composition of the collected fauna (51 species and 4,420 individuals) in the field of wheat, sugar beet and maize. Starting from the domination of geobiotic insects which spend most of their life in soil and manifest their imaginal activity on the surface of soil, the method of "Barber's traps" was applied. Within this family the dominant and subdominant species during research were Anchomenus dorsalis, Poecilus cupreus, Harpalus rufipes, Calosoma auropunctatum, Harpalu distinguendus and many others. 24 carabids species were determined using the COI mtDNA, i.e. 8 species which don't have reference sequence in the gene bank were determined and this contributes to the global bar-code database. The representatives of ground beetle family are of certain economic significance as well. They can appear as pests; on the other hand, they are known to be very beneficial i.e. as regulators of number of harmful insects as reported in numerous research papers of various authors.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Molecular Identification of Ground Beetles on Arable Land",
pages = "11364-11357",
number = "2",
volume = "21"
}

Popović, A., Mitrović, M., Petrović, M., Petrović, A., Bursić, V., Marinković, D.,& Gvozdenac, S.. (2016). Molecular Identification of Ground Beetles on Arable Land. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 21(2), 11357-11364.

Popović A, Mitrović M, Petrović M, Petrović A, Bursić V, Marinković D, Gvozdenac S. Molecular Identification of Ground Beetles on Arable Land. in Romanian Biotechnological Letters. 2016;21(2):11357-11364..

Popović, Aleksandra, Mitrović, Milana, Petrović, Miloš, Petrović, Aleksandra, Bursić, Vojislava, Marinković, Dušan, Gvozdenac, Sonja, "Molecular Identification of Ground Beetles on Arable Land" in Romanian Biotechnological Letters, 21, no. 2 (2016):11357-11364.

TY  - JOUR
AU  - Mitrović, Milana
AU  - Tomanović, Željko
AU  - Jakovljević, Miljana
AU  - Radović, D.
AU  - Havelka, J.
AU  - Stary, Petr
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/446
AB  - Populations of Liparus glabrirostris (Curculionidae: Molytinae), a weevil inhabiting higher altitudes of Central Europe, were sampled from 24 localities in the Alps and Carpathian Mountains, and the geographical structuring of genetic variation was analyzed. Comparison of the concatenated mitochondrial cytochrome oxidase subunit I and subunit II sequences revealed consistent genetic divergence between the populations of L. glabrirostris from different mountain ranges. In phylogenetic analysis using maximum parsimony and median-joining networks, concatenated mitochondrial haplotypes from the Alps and Carpathians clustered as separate lineages, with high bootstrap support. Substantial genetic distances determined between the separated groups ranged from 2.6 to 3.0%, with divergence estimated to have initiated approximately 0.85-0.98 million years ago. The nuclear elongation factor 1 alpha gene was additionally amplified and haplotype analysis showed very low evolutionary divergence (0.2%), with separate clustering as well. The observed divergence suggests that the populations have been isolated for a long time, as a consequence of environmental changes resulting in varying fragmentation of habitats in the Alps and Carpathians, interrupting genetic exchange events and altering the genetic structure of L. glabrirostris populations. On the other hand, comparison of morphological characteristics showed no differences to confirm genetically well differentiated groups of populations. A polymerase chain reaction and restriction fragment length polymorphism-based method was therefore developed to discriminate between the Alpine and Carpathian lineages.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains
EP  - 662
IS  - 5
SP  - 651
VL  - 106
DO  - 10.1017/S0007485316000377
ER  - 

@article{
author = "Mitrović, Milana and Tomanović, Željko and Jakovljević, Miljana and Radović, D. and Havelka, J. and Stary, Petr",
year = "2016",
abstract = "Populations of Liparus glabrirostris (Curculionidae: Molytinae), a weevil inhabiting higher altitudes of Central Europe, were sampled from 24 localities in the Alps and Carpathian Mountains, and the geographical structuring of genetic variation was analyzed. Comparison of the concatenated mitochondrial cytochrome oxidase subunit I and subunit II sequences revealed consistent genetic divergence between the populations of L. glabrirostris from different mountain ranges. In phylogenetic analysis using maximum parsimony and median-joining networks, concatenated mitochondrial haplotypes from the Alps and Carpathians clustered as separate lineages, with high bootstrap support. Substantial genetic distances determined between the separated groups ranged from 2.6 to 3.0%, with divergence estimated to have initiated approximately 0.85-0.98 million years ago. The nuclear elongation factor 1 alpha gene was additionally amplified and haplotype analysis showed very low evolutionary divergence (0.2%), with separate clustering as well. The observed divergence suggests that the populations have been isolated for a long time, as a consequence of environmental changes resulting in varying fragmentation of habitats in the Alps and Carpathians, interrupting genetic exchange events and altering the genetic structure of L. glabrirostris populations. On the other hand, comparison of morphological characteristics showed no differences to confirm genetically well differentiated groups of populations. A polymerase chain reaction and restriction fragment length polymorphism-based method was therefore developed to discriminate between the Alpine and Carpathian lineages.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains",
pages = "662-651",
number = "5",
volume = "106",
doi = "10.1017/S0007485316000377"
}

Mitrović, M., Tomanović, Ž., Jakovljević, M., Radović, D., Havelka, J.,& Stary, P.. (2016). Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 106(5), 651-662.
https://doi.org/10.1017/S0007485316000377

Mitrović M, Tomanović Ž, Jakovljević M, Radović D, Havelka J, Stary P. Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains. in Bulletin of Entomological Research. 2016;106(5):651-662.
doi:10.1017/S0007485316000377 .

Mitrović, Milana, Tomanović, Željko, Jakovljević, Miljana, Radović, D., Havelka, J., Stary, Petr, "Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains" in Bulletin of Entomological Research, 106, no. 5 (2016):651-662,
https://doi.org/10.1017/S0007485316000377 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/428
AB  - A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors
EP  - 630
IS  - 3
SP  - 619
VL  - 144
DO  - 10.1007/s10658-015-0800-y
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Jermini, Mauro and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
abstract = "A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors",
pages = "630-619",
number = "3",
volume = "144",
doi = "10.1007/s10658-015-0800-y"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Jermini, M., Toševski, I.,& Cvrković, T.. (2016). 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology
Springer, Dordrecht., 144(3), 619-630.
https://doi.org/10.1007/s10658-015-0800-y

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Jermini M, Toševski I, Cvrković T. 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology. 2016;144(3):619-630.
doi:10.1007/s10658-015-0800-y .

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Jermini, Mauro, Toševski, Ivo, Cvrković, Tatjana, "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors" in European Journal of Plant Pathology, 144, no. 3 (2016):619-630,
https://doi.org/10.1007/s10658-015-0800-y . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/442
AB  - The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dore, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatiotemporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in epidemiological cycles of phytoplasma-induced plant diseases.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations
EP  - 405
IS  - 3
SP  - 395
VL  - 106
DO  - 10.1017/S0007485316000067
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dore, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatiotemporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in epidemiological cycles of phytoplasma-induced plant diseases.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations",
pages = "405-395",
number = "3",
volume = "106",
doi = "10.1017/S0007485316000067"
}

Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 106(3), 395-405.
https://doi.org/10.1017/S0007485316000067

Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations. in Bulletin of Entomological Research. 2016;106(3):395-405.
doi:10.1017/S0007485316000067 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations" in Bulletin of Entomological Research, 106, no. 3 (2016):395-405,
https://doi.org/10.1017/S0007485316000067 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/431
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data
EP  - 11
IS  - 1
SP  - 9
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Krstić, Oliver and Jakovljević, Miljana and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data",
pages = "11-9",
number = "1",
volume = "66"
}

Kosovac, A., Krstić, O., Jakovljević, M., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 9-11.

Kosovac A, Krstić O, Jakovljević M, Cvrković T, Mitrović M, Toševski I, Jović J. Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg. 2016;66(1):9-11..

Kosovac, Andrea, Krstić, Oliver, Jakovljević, Miljana, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):9-11.

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/432
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia
EP  - 18
IS  - 1
SP  - 17
VL  - 66
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia",
pages = "18-17",
number = "1",
volume = "66"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Toševski, I.,& Cvrković, T.. (2016). Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 17-18.

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Toševski I, Cvrković T. Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg. 2016;66(1):17-18..

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Toševski, Ivo, Cvrković, Tatjana, "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):17-18.

TY  - JOUR
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Mitrović, Milana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/433
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland
EP  - 23
IS  - 1
SP  - 22
VL  - 66
ER  - 

@article{
author = "Trivellone, Valeria and Jermini, Mauro and Jović, Jelena and Cvrković, Tatjana and Jakovljević, Miljana and Kosovac, Andrea and Krstić, Oliver and Toševski, Ivo and Mitrović, Milana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland",
pages = "23-22",
number = "1",
volume = "66"
}

Trivellone, V., Jermini, M., Jović, J., Cvrković, T., Jakovljević, M., Kosovac, A., Krstić, O., Toševski, I.,& Mitrović, M.. (2016). Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 22-23.

Trivellone V, Jermini M, Jović J, Cvrković T, Jakovljević M, Kosovac A, Krstić O, Toševski I, Mitrović M. Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg. 2016;66(1):22-23..

Trivellone, Valeria, Jermini, Mauro, Jović, Jelena, Cvrković, Tatjana, Jakovljević, Miljana, Kosovac, Andrea, Krstić, Oliver, Toševski, Ivo, Mitrović, Milana, "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):22-23.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/434
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe
EP  - 25
IS  - 1
SP  - 24
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe",
pages = "25-24",
number = "1",
volume = "66"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2016). Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 24-25.

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg. 2016;66(1):24-25..

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):24-25.

TY  - JOUR
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/444
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro
EP  - 1775
IS  - 8
SP  - 1775
VL  - 100
DO  - 10.1094/PDIS-02-16-0180-PDN
ER  - 

@article{
author = "Radonjić, Sanja and Hrnčić, Snježana and Kosovac, Andrea and Krstić, Oliver and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro",
pages = "1775-1775",
number = "8",
volume = "100",
doi = "10.1094/PDIS-02-16-0180-PDN"
}

Radonjić, S., Hrnčić, S., Kosovac, A., Krstić, O., Mitrović, M., Jović, J.,& Toševski, I.. (2016). First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(8), 1775-1775.
https://doi.org/10.1094/PDIS-02-16-0180-PDN

Radonjić S, Hrnčić S, Kosovac A, Krstić O, Mitrović M, Jović J, Toševski I. First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro. in Plant Disease. 2016;100(8):1775-1775.
doi:10.1094/PDIS-02-16-0180-PDN .

Radonjić, Sanja, Hrnčić, Snježana, Kosovac, Andrea, Krstić, Oliver, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro" in Plant Disease, 100, no. 8 (2016):1775-1775,
https://doi.org/10.1094/PDIS-02-16-0180-PDN . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Milosavljević, Anja
AU  - Bosković, J.
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/455
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia
EP  - 221
IS  - 1
SP  - 221
VL  - 100
DO  - 10.1094/PDIS-02-15-0209-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Pfaf-Dolovac, Erika and Milosavljević, Anja and Bosković, J. and Jović, Jelena and Mitrović, Milana and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia",
pages = "221-221",
number = "1",
volume = "100",
doi = "10.1094/PDIS-02-15-0209-PDN"
}

Trkulja, N., Pfaf-Dolovac, E., Milosavljević, A., Bosković, J., Jović, J., Mitrović, M.,& Toševski, I.. (2016). First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 221-221.
https://doi.org/10.1094/PDIS-02-15-0209-PDN

Trkulja N, Pfaf-Dolovac E, Milosavljević A, Bosković J, Jović J, Mitrović M, Toševski I. First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease. 2016;100(1):221-221.
doi:10.1094/PDIS-02-15-0209-PDN .

Trkulja, Nenad, Pfaf-Dolovac, Erika, Milosavljević, Anja, Bosković, J., Jović, Jelena, Mitrović, Milana, Toševski, Ivo, "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia" in Plant Disease, 100, no. 1 (2016):221-221,
https://doi.org/10.1094/PDIS-02-15-0209-PDN . .

TY  - JOUR
AU  - Jamhour, Aiman
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Stary, Petr
AU  - Tomanović, Željko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/435
AB  - Here we tested Aphidius urticae s. str. host-associated lineages from Microlophium carnosum (Buckton), Amphorophora rubi (Kaltenbach), Macrosiphum funestum (Macchiati) and Aulacorthum vaccinii Hille Ris Lambers with the barcoding region of the mitochondrial cytochrome oxidase subunit I gene used to analyse population differences and elucidate phylogenetic relationships between the separated taxa. This molecular marker has been shown to be the most informative molecular marker in resolving species complexes in aphidiine parasitoids. Analyses of the mitochondrial sequences revealed the existence of three clearly separated mitochondrial lineages of A. urticae s. str. group associated with: i) Macrosiphum funestum and Aulacorthum vaccinii aphid hosts, ii) Microlophium carnosum and iii) Amphorophora rubi. This corresponds to the initial descriptions of A. rubi, A. silvaticus and A. urticae and their aphid host associations prior to synonymization of A. rubi and A. silvaticus with A. urticae. On the other hand, significant evolutionary distances ranging from 2.3 to 9.2% between the three mitochondrial lineages were not accompanied by clear morphological differences. Therefore, re-descriptions of A. rubi and A. silvaticus are presented, together with their morphological differentiation in a key, as well as their phylogenetic relationships and genetical differentiation.
PB  - Magnolia Press, Auckland
T2  - Zootaxa
T1  - Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae)
EP  - 288
IS  - 2
SP  - 278
VL  - 4178
DO  - 10.11646/zootaxa.4178.2.6
ER  - 

@article{
author = "Jamhour, Aiman and Mitrović, Milana and Petrović, Anđeljko and Stary, Petr and Tomanović, Željko",
year = "2016",
abstract = "Here we tested Aphidius urticae s. str. host-associated lineages from Microlophium carnosum (Buckton), Amphorophora rubi (Kaltenbach), Macrosiphum funestum (Macchiati) and Aulacorthum vaccinii Hille Ris Lambers with the barcoding region of the mitochondrial cytochrome oxidase subunit I gene used to analyse population differences and elucidate phylogenetic relationships between the separated taxa. This molecular marker has been shown to be the most informative molecular marker in resolving species complexes in aphidiine parasitoids. Analyses of the mitochondrial sequences revealed the existence of three clearly separated mitochondrial lineages of A. urticae s. str. group associated with: i) Macrosiphum funestum and Aulacorthum vaccinii aphid hosts, ii) Microlophium carnosum and iii) Amphorophora rubi. This corresponds to the initial descriptions of A. rubi, A. silvaticus and A. urticae and their aphid host associations prior to synonymization of A. rubi and A. silvaticus with A. urticae. On the other hand, significant evolutionary distances ranging from 2.3 to 9.2% between the three mitochondrial lineages were not accompanied by clear morphological differences. Therefore, re-descriptions of A. rubi and A. silvaticus are presented, together with their morphological differentiation in a key, as well as their phylogenetic relationships and genetical differentiation.",
publisher = "Magnolia Press, Auckland",
journal = "Zootaxa",
title = "Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae)",
pages = "288-278",
number = "2",
volume = "4178",
doi = "10.11646/zootaxa.4178.2.6"
}

Jamhour, A., Mitrović, M., Petrović, A., Stary, P.,& Tomanović, Ž.. (2016). Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae). in Zootaxa
Magnolia Press, Auckland., 4178(2), 278-288.
https://doi.org/10.11646/zootaxa.4178.2.6

Jamhour A, Mitrović M, Petrović A, Stary P, Tomanović Ž. Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae). in Zootaxa. 2016;4178(2):278-288.
doi:10.11646/zootaxa.4178.2.6 .

Jamhour, Aiman, Mitrović, Milana, Petrović, Anđeljko, Stary, Petr, Tomanović, Željko, "Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae)" in Zootaxa, 4178, no. 2 (2016):278-288,
https://doi.org/10.11646/zootaxa.4178.2.6 . .

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Mitrovski-Bogdanović, Ana S
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/418
AB  - Background: Morphological divergence often increases with phylogenetic distance, thus making morphology taxonomically informative. However, transitions to asexual reproduction may complicate this relationship because asexual lineages capture and freeze parts of the phenotypic variation of the sexual populations from which they derive. Parasitoid wasps belonging to the genus Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) are composed of over 20 species that exploit over a hundred species of aphid hosts, including many important agricultural pests. Within Lysiphlebus, two genetically and morphologically well- defined species groups are recognised: the "fabarum" and the "testaceipes" groups. Yet within each group, sexual as well as asexual lineages occur, and in L. fabarum different morphs of unknown origin and status have been recognised. In this study, we selected a broad sample of specimens from the genus Lysiphlebus to explore the relationship between genetic divergence, reproductive mode and morphological variation in wing size and shape (quantified by geometric morphometrics). Results: The analyses of mitochondrial and nuclear gene sequences revealed a clear separation between the "testaceipes" and "fabarum" groups of Lysiphlebus, as well as three well-defined phylogenetic lineages within the "fabarum" species group and two lineages within the "testaceipes" group. Divergence in wing shape was concordant with the deep split between the "testaceipes" and "fabarum" species groups, but within groups no clear association between genetic divergence and wing shape variation was observed. On the other hand, we found significant and consistent differences in the shape of the wing between sexual and asexual lineages, even when they were closely related. Conclusions: Mapping wing shape data onto an independently derived molecular phylogeny of Lysiphlebus revealed an association between genetic and morphological divergence only for the deepest phylogenetic split. In more recently diverged taxa, much of the variation in wing shape was explained by differences between sexual and asexual lineages, suggesting a mechanistic link between wing shape and reproductive mode in these parasitoid wasps.
PB  - BMC
T2  - BMC Evolutionary Biology
T1  - Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode
VL  - 15
DO  - 10.1186/s12862-015-0293-5
ER  - 

@article{
author = "Petrović, Anđeljko and Mitrović, Milana and Ivanović, Ana and Zikić, Vladimir and Kavallieratos, Nickolas G. and Stary, Petr and Mitrovski-Bogdanović, Ana S and Tomanović, Željko and Vorburger, Christoph",
year = "2015",
abstract = "Background: Morphological divergence often increases with phylogenetic distance, thus making morphology taxonomically informative. However, transitions to asexual reproduction may complicate this relationship because asexual lineages capture and freeze parts of the phenotypic variation of the sexual populations from which they derive. Parasitoid wasps belonging to the genus Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) are composed of over 20 species that exploit over a hundred species of aphid hosts, including many important agricultural pests. Within Lysiphlebus, two genetically and morphologically well- defined species groups are recognised: the "fabarum" and the "testaceipes" groups. Yet within each group, sexual as well as asexual lineages occur, and in L. fabarum different morphs of unknown origin and status have been recognised. In this study, we selected a broad sample of specimens from the genus Lysiphlebus to explore the relationship between genetic divergence, reproductive mode and morphological variation in wing size and shape (quantified by geometric morphometrics). Results: The analyses of mitochondrial and nuclear gene sequences revealed a clear separation between the "testaceipes" and "fabarum" groups of Lysiphlebus, as well as three well-defined phylogenetic lineages within the "fabarum" species group and two lineages within the "testaceipes" group. Divergence in wing shape was concordant with the deep split between the "testaceipes" and "fabarum" species groups, but within groups no clear association between genetic divergence and wing shape variation was observed. On the other hand, we found significant and consistent differences in the shape of the wing between sexual and asexual lineages, even when they were closely related. Conclusions: Mapping wing shape data onto an independently derived molecular phylogeny of Lysiphlebus revealed an association between genetic and morphological divergence only for the deepest phylogenetic split. In more recently diverged taxa, much of the variation in wing shape was explained by differences between sexual and asexual lineages, suggesting a mechanistic link between wing shape and reproductive mode in these parasitoid wasps.",
publisher = "BMC",
journal = "BMC Evolutionary Biology",
title = "Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode",
volume = "15",
doi = "10.1186/s12862-015-0293-5"
}

Petrović, A., Mitrović, M., Ivanović, A., Zikić, V., Kavallieratos, N. G., Stary, P., Mitrovski-Bogdanović, A. S., Tomanović, Ž.,& Vorburger, C.. (2015). Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode. in BMC Evolutionary Biology
BMC., 15.
https://doi.org/10.1186/s12862-015-0293-5

Petrović A, Mitrović M, Ivanović A, Zikić V, Kavallieratos NG, Stary P, Mitrovski-Bogdanović AS, Tomanović Ž, Vorburger C. Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode. in BMC Evolutionary Biology. 2015;15.
doi:10.1186/s12862-015-0293-5 .

Petrović, Anđeljko, Mitrović, Milana, Ivanović, Ana, Zikić, Vladimir, Kavallieratos, Nickolas G., Stary, Petr, Mitrovski-Bogdanović, Ana S, Tomanović, Željko, Vorburger, Christoph, "Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode" in BMC Evolutionary Biology, 15 (2015),
https://doi.org/10.1186/s12862-015-0293-5 . .

TY  - JOUR
AU  - Atanasova, Biljana
AU  - Jakovljević, Miljana
AU  - Spasov, Dušan
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/383
AB  - Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia
EP  - 770
IS  - 4
SP  - 759
VL  - 142
DO  - 10.1007/s10658-015-0649-0
ER  - 

@article{
author = "Atanasova, Biljana and Jakovljević, Miljana and Spasov, Dušan and Jović, Jelena and Mitrović, Milana and Toševski, Ivo and Cvrković, Tatjana",
year = "2015",
abstract = "Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia",
pages = "770-759",
number = "4",
volume = "142",
doi = "10.1007/s10658-015-0649-0"
}

Atanasova, B., Jakovljević, M., Spasov, D., Jović, J., Mitrović, M., Toševski, I.,& Cvrković, T.. (2015). The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology
Springer, Dordrecht., 142(4), 759-770.
https://doi.org/10.1007/s10658-015-0649-0

Atanasova B, Jakovljević M, Spasov D, Jović J, Mitrović M, Toševski I, Cvrković T. The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology. 2015;142(4):759-770.
doi:10.1007/s10658-015-0649-0 .

Atanasova, Biljana, Jakovljević, Miljana, Spasov, Dušan, Jović, Jelena, Mitrović, Milana, Toševski, Ivo, Cvrković, Tatjana, "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia" in European Journal of Plant Pathology, 142, no. 4 (2015):759-770,
https://doi.org/10.1007/s10658-015-0649-0 . .