TY  - JOUR
AU  - Stefani, Emilio
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Altin, Irem
AU  - Nagy, Ildikó K.
AU  - Kovács, Tamás
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5851
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/610
AB  - Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.
PB  - MDPI
T2  - Microorganisms
T1  - Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future
IS  - 5
SP  - 1056
VL  - 9
DO  - 10.3390/microorganisms9051056
ER  - 

@article{
author = "Stefani, Emilio and Obradović, Aleksa and Gašić, Katarina and Altin, Irem and Nagy, Ildikó K. and Kovács, Tamás",
year = "2021",
abstract = "Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future",
number = "5",
pages = "1056",
volume = "9",
doi = "10.3390/microorganisms9051056"
}

Stefani, E., Obradović, A., Gašić, K., Altin, I., Nagy, I. K.,& Kovács, T.. (2021). Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms
MDPI., 9(5), 1056.
https://doi.org/10.3390/microorganisms9051056

Stefani E, Obradović A, Gašić K, Altin I, Nagy IK, Kovács T. Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms. 2021;9(5):1056.
doi:10.3390/microorganisms9051056 .

Stefani, Emilio, Obradović, Aleksa, Gašić, Katarina, Altin, Irem, Nagy, Ildikó K., Kovács, Tamás, "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future" in Microorganisms, 9, no. 5 (2021):1056,
https://doi.org/10.3390/microorganisms9051056 . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/576
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/563
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/597
AB  - Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species.
AB  - Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of cucurbits
T1  - Bakterioze tikava
EP  - 445
IS  - 6
SP  - 431
VL  - 47
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species., Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of cucurbits, Bakterioze tikava",
pages = "445-431",
number = "6",
volume = "47"
}

Zlatković, N., Gašić, K., Prokić, A.,& Obradović, A.. (2019). Bacterial diseases of cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 47(6), 431-445.

Zlatković N, Gašić K, Prokić A, Obradović A. Bacterial diseases of cucurbits. in Biljni lekar. 2019;47(6):431-445..

Zlatković, Nevena, Gašić, Katarina, Prokić, Andjelka, Obradović, Aleksa, "Bacterial diseases of cucurbits" in Biljni lekar, 47, no. 6 (2019):431-445.

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/601
AB  - Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material
EP  - 1052
IS  - 3
SP  - 1039
VL  - 51
DO  - 10.2298/GENSR1903039I
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Gašić, Katarina and Prokić, Andjelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2019",
abstract = "Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material",
pages = "1052-1039",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903039I"
}

Ivanović, M., Kuzmanović, N., Gašić, K., Prokić, A., Zlatković, N.,& Obradović, A.. (2019). Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika
Društvo genetičara Srbije, Beograd., 51(3), 1039-1052.
https://doi.org/10.2298/GENSR1903039I

Ivanović M, Kuzmanović N, Gašić K, Prokić A, Zlatković N, Obradović A. Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika. 2019;51(3):1039-1052.
doi:10.2298/GENSR1903039I .

Ivanović, Milan, Kuzmanović, Nemanja, Gašić, Katarina, Prokić, Andjelka, Zlatković, Nevena, Obradović, Aleksa, "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material" in Genetika, 51, no. 3 (2019):1039-1052,
https://doi.org/10.2298/GENSR1903039I . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/508
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media S.A.
T2  - Frontiers In Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 

@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Anđelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers In Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}

Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers In Microbiology
Frontiers Media S.A.., 9.
https://doi.org/10.3389/fmicb.2018.02021

Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers In Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .

Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Anđelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers In Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .

TY  - JOUR
AU  - Krivokapić, Marija
AU  - Gavrilović, Veljko
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Fira, Đorđe
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/545
AB  - The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.
AB  - Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia
T1  - Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji
EP  - 184
IS  - 3-4
SP  - 175
VL  - 33
DO  - 10.2298/PIF1804175K
ER  - 

@article{
author = "Krivokapić, Marija and Gavrilović, Veljko and Ivanović, Milan and Kuzmanović, Nemanja and Fira, Đorđe and Obradović, Aleksa and Gašić, Katarina",
year = "2018",
abstract = "The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation., Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia, Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji",
pages = "184-175",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804175K"
}

Krivokapić, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, Đ., Obradović, A.,& Gašić, K.. (2018). Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 175-184.
https://doi.org/10.2298/PIF1804175K

Krivokapić M, Gavrilović V, Ivanović M, Kuzmanović N, Fira Đ, Obradović A, Gašić K. Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina. 2018;33(3-4):175-184.
doi:10.2298/PIF1804175K .

Krivokapić, Marija, Gavrilović, Veljko, Ivanović, Milan, Kuzmanović, Nemanja, Fira, Đorđe, Obradović, Aleksa, Gašić, Katarina, "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):175-184,
https://doi.org/10.2298/PIF1804175K . .

TY  - CONF
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, M.
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/522
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot
EP  - 133
IS  - 10
SP  - 132
VL  - 108
ER  - 

@conference{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, M. and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot",
pages = "133-132",
number = "10",
volume = "108"
}

Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N.,& Obradović, A.. (2018). Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 132-133.

Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Obradović A. Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology. 2018;108(10):132-133..

Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, M., Zlatković, Nevena, Obradović, Aleksa, "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot" in Phytopathology, 108, no. 10 (2018):132-133.

TY  - CONF
AU  - Prokić, Anđelka
AU  - Ivanović, M.
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/521
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro
EP  - 24
IS  - 10
SP  - 24
VL  - 108
ER  - 

@conference{
author = "Prokić, Anđelka and Ivanović, M. and Gašić, Katarina and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro",
pages = "24-24",
number = "10",
volume = "108"
}

Prokić, A., Ivanović, M., Gašić, K., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2018). Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 24-24.

Prokić A, Ivanović M, Gašić K, Kuzmanović N, Zlatković N, Obradović A. Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology. 2018;108(10):24-24..

Prokić, Anđelka, Ivanović, M., Gašić, Katarina, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro" in Phytopathology, 108, no. 10 (2018):24-24.

TY  - JOUR
AU  - Gašić, Katarina
AU  - Pavlović, Z.
AU  - Santander, R. D.
AU  - Meredith, C.
AU  - Acimović, S. G.
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/510
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. syringae Associated with Bacterial Blossom Blast on Apple (Mains pumila) in the United States
EP  - 1848
IS  - 9
SP  - 1848
VL  - 102
DO  - 10.1094/PDIS-01-18-0184-PDN
ER  - 

@article{
author = "Gašić, Katarina and Pavlović, Z. and Santander, R. D. and Meredith, C. and Acimović, S. G.",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. syringae Associated with Bacterial Blossom Blast on Apple (Mains pumila) in the United States",
pages = "1848-1848",
number = "9",
volume = "102",
doi = "10.1094/PDIS-01-18-0184-PDN"
}

Gašić, K., Pavlović, Z., Santander, R. D., Meredith, C.,& Acimović, S. G.. (2018). First Report of Pseudomonas syringae pv. syringae Associated with Bacterial Blossom Blast on Apple (Mains pumila) in the United States. in Plant Disease
Amer Phytopathological Soc, St Paul., 102(9), 1848-1848.
https://doi.org/10.1094/PDIS-01-18-0184-PDN

Gašić K, Pavlović Z, Santander RD, Meredith C, Acimović SG. First Report of Pseudomonas syringae pv. syringae Associated with Bacterial Blossom Blast on Apple (Mains pumila) in the United States. in Plant Disease. 2018;102(9):1848-1848.
doi:10.1094/PDIS-01-18-0184-PDN .

Gašić, Katarina, Pavlović, Z., Santander, R. D., Meredith, C., Acimović, S. G., "First Report of Pseudomonas syringae pv. syringae Associated with Bacterial Blossom Blast on Apple (Mains pumila) in the United States" in Plant Disease, 102, no. 9 (2018):1848-1848,
https://doi.org/10.1094/PDIS-01-18-0184-PDN . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/501
AB  - Cucurbits production has a long tradition in our country. In some parts of the country, cucumber, watermelon and melon are the most important agricultural crops. High yield and good profit are often compromized by various biotic and abiotic factors. In years with favorable weather conditions cucurbit bacterial diseases can cause serious damage. This paper describes major bacterial diseases of cucurbit plants, such as angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial fruit blotch caused by Acidovorax citrulli, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila and cucurbit yellow wine disease caused by Serratia marcescens, relatively new and invasive disease across the United States. In addition, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae and Pectobacterium carotovorum subsp. carotovorum, inducing bacterial soft rot, are becoming more important in cucurbit production worldwide.
AB  - Biljke familije Cucurbitaceae se dugi niz godina gaje širom naše zemlje. U pojedinim krajevima, proizvodnja krastavca, lubenice i dinje predstavlja osnovni izvor prihoda za mnoga poljoprivredna domaćinstva. Visok prinos i ekonomsku dobit često ugrožavaju različiti biotski i abiotski faktori. U godinama sa uslovima povoljnim za nastanak infekcije, bakteriozna oboljenja mogu prouzrokovati velike gubitke. U ovom radu predstavljene su najznačajnije bakterioze biljaka familije Cucurbitaceae kao što su uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli; zatim bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila i žutilo vreža, čiji je prouzrokovač Serratia marcescens. Gubicima u proizvodnji doprinose i prouzrokovači bakteriozne pegavosti, Pseudomonas syringae pv. syringae, kao i bakteriozne vlažne truleži, Pectobacterium carotovorum subsp. carotovorum.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of Cucurbits
T1  - Bakteriozna oboljenja biljaka familije Cucurbitaceae
EP  - 400
IS  - 4
SP  - 390
VL  - 45
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Anđelka and Kuzmanović, Nemanja and Gašić, Katarina and Ivanović, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Cucurbits production has a long tradition in our country. In some parts of the country, cucumber, watermelon and melon are the most important agricultural crops. High yield and good profit are often compromized by various biotic and abiotic factors. In years with favorable weather conditions cucurbit bacterial diseases can cause serious damage. This paper describes major bacterial diseases of cucurbit plants, such as angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial fruit blotch caused by Acidovorax citrulli, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila and cucurbit yellow wine disease caused by Serratia marcescens, relatively new and invasive disease across the United States. In addition, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae and Pectobacterium carotovorum subsp. carotovorum, inducing bacterial soft rot, are becoming more important in cucurbit production worldwide., Biljke familije Cucurbitaceae se dugi niz godina gaje širom naše zemlje. U pojedinim krajevima, proizvodnja krastavca, lubenice i dinje predstavlja osnovni izvor prihoda za mnoga poljoprivredna domaćinstva. Visok prinos i ekonomsku dobit često ugrožavaju različiti biotski i abiotski faktori. U godinama sa uslovima povoljnim za nastanak infekcije, bakteriozna oboljenja mogu prouzrokovati velike gubitke. U ovom radu predstavljene su najznačajnije bakterioze biljaka familije Cucurbitaceae kao što su uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli; zatim bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila i žutilo vreža, čiji je prouzrokovač Serratia marcescens. Gubicima u proizvodnji doprinose i prouzrokovači bakteriozne pegavosti, Pseudomonas syringae pv. syringae, kao i bakteriozne vlažne truleži, Pectobacterium carotovorum subsp. carotovorum.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of Cucurbits, Bakteriozna oboljenja biljaka familije Cucurbitaceae",
pages = "400-390",
number = "4",
volume = "45"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Ivanović, M.,& Obradović, A.. (2017). Bacterial diseases of Cucurbits. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(4), 390-400.

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Ivanović M, Obradović A. Bacterial diseases of Cucurbits. in Biljni lekar. 2017;45(4):390-400..

Zlatković, Nevena, Prokić, Anđelka, Kuzmanović, Nemanja, Gašić, Katarina, Ivanović, Milan, Obradović, Aleksa, "Bacterial diseases of Cucurbits" in Biljni lekar, 45, no. 4 (2017):390-400.

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/504
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45"
}

Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.

Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596..

Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596.

TY  - JOUR
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/503
AB  - Bacterial diseases are very common in tomato and pepper crops, especially under favourable weather conditions. One of the economically most important pathogens that affect tomato production worldwide is Ralstonia solanacearum, causal agent of bacterial wilt of tomato. The pathogen survives in soil and water. It colonizes xylem, clogs the vascular tissue preventing water movement throughout the stem, causing colapse of the infected plants. In addition, wilting of tomato and pepper can be caused by Candidatus Phytoplasma solani (Stolbur Phytoplasma). Main sources of infection with this pathogen are wild plants from where it is further transmitted by feeding of leafhopper vectors. The aim of this paper is to point to distribution and economic importance of those diseases, as well as characteristics of the pathogens. Information about symptoms and epidemiology of the disease are important for an accurate disease diagnosis and pathogen identification, which contributes to the timely and successful protection strategy.
AB  - Oboljenja bakteriozne prirode veoma su česta u usevima paradajza i paprike, posebno kada vremenski uslovi pogoduju nastanku i širenju infekcije. Među ekonomski najznačajnijim bakterijama koje ugrožavaju proizvodnju paradajza u svetu, izdvaja se prouzrokovač bakteriozne uvelosti paradajza Ralstonia solanacearum. Patogen se dugo održava u zemljištu i vodi. Naseljava ksilem biljaka onemogućavajući protok vode, usled čega dolazi do pojave uvelosti. Osim ovog patogena, simptome uvelosti paradajza i paprike može takođe prouzrokovati Candidatus Phytoplasma solani (Stolbur fitoplazma). Ovo oboljenje prenosi se cikadama, dok su glavni izvori infekcije korovske biljke. Cilj ovog rada je da se prikaže rasprostranjenost i ekonomski značaj navedenih oboljenja paradajza i paprike, kao i osnovne karakteristike njihovih prouzrokovača. Poznavanje simptomatologije i epidemiologije oboljenja je od posebnog značaja za pravilnu dijagnozu oboljenja i identifikaciju patogena, što doprinosi pravovremenoj i uspešnoj zaštiti.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial wilt of tomato and Stolbur of tomato and pepper
T1  - Prouzrokovači bakteriozne uvelosti paradajza i žute uvelosti (Stolbur) paprike i paradajza
EP  - 586
IS  - 6
SP  - 575
VL  - 45
ER  - 

@article{
author = "Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial diseases are very common in tomato and pepper crops, especially under favourable weather conditions. One of the economically most important pathogens that affect tomato production worldwide is Ralstonia solanacearum, causal agent of bacterial wilt of tomato. The pathogen survives in soil and water. It colonizes xylem, clogs the vascular tissue preventing water movement throughout the stem, causing colapse of the infected plants. In addition, wilting of tomato and pepper can be caused by Candidatus Phytoplasma solani (Stolbur Phytoplasma). Main sources of infection with this pathogen are wild plants from where it is further transmitted by feeding of leafhopper vectors. The aim of this paper is to point to distribution and economic importance of those diseases, as well as characteristics of the pathogens. Information about symptoms and epidemiology of the disease are important for an accurate disease diagnosis and pathogen identification, which contributes to the timely and successful protection strategy., Oboljenja bakteriozne prirode veoma su česta u usevima paradajza i paprike, posebno kada vremenski uslovi pogoduju nastanku i širenju infekcije. Među ekonomski najznačajnijim bakterijama koje ugrožavaju proizvodnju paradajza u svetu, izdvaja se prouzrokovač bakteriozne uvelosti paradajza Ralstonia solanacearum. Patogen se dugo održava u zemljištu i vodi. Naseljava ksilem biljaka onemogućavajući protok vode, usled čega dolazi do pojave uvelosti. Osim ovog patogena, simptome uvelosti paradajza i paprike može takođe prouzrokovati Candidatus Phytoplasma solani (Stolbur fitoplazma). Ovo oboljenje prenosi se cikadama, dok su glavni izvori infekcije korovske biljke. Cilj ovog rada je da se prikaže rasprostranjenost i ekonomski značaj navedenih oboljenja paradajza i paprike, kao i osnovne karakteristike njihovih prouzrokovača. Poznavanje simptomatologije i epidemiologije oboljenja je od posebnog značaja za pravilnu dijagnozu oboljenja i identifikaciju patogena, što doprinosi pravovremenoj i uspešnoj zaštiti.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial wilt of tomato and Stolbur of tomato and pepper, Prouzrokovači bakteriozne uvelosti paradajza i žute uvelosti (Stolbur) paprike i paradajza",
pages = "586-575",
number = "6",
volume = "45"
}

Gašić, K., Šević, M.,& Obradović, A.. (2017). Bacterial wilt of tomato and Stolbur of tomato and pepper. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 575-586.

Gašić K, Šević M, Obradović A. Bacterial wilt of tomato and Stolbur of tomato and pepper. in Biljni lekar. 2017;45(6):575-586..

Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Bacterial wilt of tomato and Stolbur of tomato and pepper" in Biljni lekar, 45, no. 6 (2017):575-586.

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/505
AB  - Bacterial spot of pepper and tomato caused by Xanthomonas species, is one of the widespread and economically most important diseases of pepper and tomato. Due to the lack of resistant pepper and tomato genotypes, the emergence of new races of bacteria, and strains resistant to copper compounds, questionable seed quality and limited control practices, thesebacteria are limiting pepper production in Serbia. On tomato plants, under favourable weather conditions for disease development, these bacteria can cause major damage reflected in overall yield as well as in fruit quality. The protection of pepper and tomato, based on chemical methods such as use of copper-based compounds in combination with ethylene-bis-dithiocarbamates, do not provide a satisfactory disease control. Use of antibiotics in plant protection is not permitted in the EU as well as in Serbia. However, results of many authors show that satisfactory control of bacterial spot can be achieved by integrating positive effect of chemical treatments (copper compounds, systemic resistance activators) and various biological methods (antagonists, bacteriophages). However, nature of biological agents, as well as the specific mechanism of action of the systemic resistance activators, require careful optimization of the time and number of treatments in order to achieve maximum efficiency.
AB  - Bakteriozna pegavost lista i krastavost plodova prouzrokovana Xanthomonas vrstama, je jedna od najrasprostranjenijih i ekonomski najznačajnijih bolesti paprike i paradajza u svetu. Usled nedostatka otpornih genotipova paprike i paradajza, pojave novih rasa bakterije, sojeva rezistentnih prema jedinjenjima bakra, sumnjivog kvaliteta semena i ograničenih mera kontrole, ova bakterioza predstavlja ograničavajući faktor proizvodnje paprike u Srbiji. Kada vremenski uslovi pogoduju razvoju bolesti na biljkama paradajza, ova bakterioza može prouzrokovati velike gubitke usled smanjenja ukupnog prinosa i kvaliteta plodova koji nisu pogodni za industrijsku preradu. U zaštiti paprike i paradajza kod nas dominiraju hemijske mere borbe, odnosno korišćenje preparata na bazi jedinjenja bakra u kombinaciji sa etilenbis-ditiokarbamatima koji ne obezbeđuju zadovoljavajući efekat zaštite. Antibiotici se ne mogu primenjivati usled zakonskih ograničenja u Evropskoj uniji. Integracijom pozitivnog efekta hemijskih metoda (bakarni preparati, aktivatori sistemične otpornosti) i različitih bioloških metoda (antagonostički sojevi bakterija, bakteriofagi) može se postići zadovoljavajući efekat zaštite. Priroda bioloških agenasa, kao i specifičan mehanizam dejstva aktivatora sistemične otpornosti, zahtevaju pažljivu optimizaciju vremena i broja tretmana kako bi se postigla maksimalna efikasnost.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Control of pepper and tomato bacterial spot
T1  - Zaštita paprike i paradajza od prouzrokovača bakteriozne pegavosti lista i krastavosti plodova
EP  - 609
IS  - 6
SP  - 596
VL  - 45
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato caused by Xanthomonas species, is one of the widespread and economically most important diseases of pepper and tomato. Due to the lack of resistant pepper and tomato genotypes, the emergence of new races of bacteria, and strains resistant to copper compounds, questionable seed quality and limited control practices, thesebacteria are limiting pepper production in Serbia. On tomato plants, under favourable weather conditions for disease development, these bacteria can cause major damage reflected in overall yield as well as in fruit quality. The protection of pepper and tomato, based on chemical methods such as use of copper-based compounds in combination with ethylene-bis-dithiocarbamates, do not provide a satisfactory disease control. Use of antibiotics in plant protection is not permitted in the EU as well as in Serbia. However, results of many authors show that satisfactory control of bacterial spot can be achieved by integrating positive effect of chemical treatments (copper compounds, systemic resistance activators) and various biological methods (antagonists, bacteriophages). However, nature of biological agents, as well as the specific mechanism of action of the systemic resistance activators, require careful optimization of the time and number of treatments in order to achieve maximum efficiency., Bakteriozna pegavost lista i krastavost plodova prouzrokovana Xanthomonas vrstama, je jedna od najrasprostranjenijih i ekonomski najznačajnijih bolesti paprike i paradajza u svetu. Usled nedostatka otpornih genotipova paprike i paradajza, pojave novih rasa bakterije, sojeva rezistentnih prema jedinjenjima bakra, sumnjivog kvaliteta semena i ograničenih mera kontrole, ova bakterioza predstavlja ograničavajući faktor proizvodnje paprike u Srbiji. Kada vremenski uslovi pogoduju razvoju bolesti na biljkama paradajza, ova bakterioza može prouzrokovati velike gubitke usled smanjenja ukupnog prinosa i kvaliteta plodova koji nisu pogodni za industrijsku preradu. U zaštiti paprike i paradajza kod nas dominiraju hemijske mere borbe, odnosno korišćenje preparata na bazi jedinjenja bakra u kombinaciji sa etilenbis-ditiokarbamatima koji ne obezbeđuju zadovoljavajući efekat zaštite. Antibiotici se ne mogu primenjivati usled zakonskih ograničenja u Evropskoj uniji. Integracijom pozitivnog efekta hemijskih metoda (bakarni preparati, aktivatori sistemične otpornosti) i različitih bioloških metoda (antagonostički sojevi bakterija, bakteriofagi) može se postići zadovoljavajući efekat zaštite. Priroda bioloških agenasa, kao i specifičan mehanizam dejstva aktivatora sistemične otpornosti, zahtevaju pažljivu optimizaciju vremena i broja tretmana kako bi se postigla maksimalna efikasnost.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Control of pepper and tomato bacterial spot, Zaštita paprike i paradajza od prouzrokovača bakteriozne pegavosti lista i krastavosti plodova",
pages = "609-596",
number = "6",
volume = "45"
}

Šević, M., Gašić, K., Ignjatov, M.,& Obradović, A.. (2017). Control of pepper and tomato bacterial spot. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 596-609.

Šević M, Gašić K, Ignjatov M, Obradović A. Control of pepper and tomato bacterial spot. in Biljni lekar. 2017;45(6):596-609..

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Obradović, Aleksa, "Control of pepper and tomato bacterial spot" in Biljni lekar, 45, no. 6 (2017):596-609.

TY  - JOUR
AU  - Radunović, Dragana
AU  - Gavrilović, Veljko
AU  - Gašić, Katarina
AU  - Paunović, M.
AU  - Stojšin, Vera
AU  - Grahovac, M.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/484
AB  - In the period from 2012-2015 plant samples with fireblight symptoms were collected from pome fruits and indigenous plant, in main fruitgrowing regions of Montenegro. After succesfull isolation, pathogenicity of the obtained strains was tested by artificial inoculation of immature pear fruits, variety Viljamovka. Hypersensitive reaction was tested on tobacco leaves, variety White Burley. Identification and genetic diversity studies were performed using several molecular techniques on 18 Erwinia amylovora strains originating from quince, pear, apple and hawthorn. Bacterial identity was confirmed by nested PCR in which all studied strains produced the expected amplification fragment of plasmid pEA29. To detect potential genetic variations in E. amylovora population, rep-PCR was conducted. Using REP, ERIC and BOX primers, in all three PCR reactions, differences between studied strains were detected, i.e. pear strains had different genetic profiles from all other studied strains, including reference strain. Genetic variability of selected E. amylovora strains was studied by RAPD-PCR as well. Both of the used random primers, CUGEA-3 and CUGEA-5, showed discriminatory potential by separating genetic profiles of pear strains from all other studied strains, including reference strain. This is the first study of genetic variability of E. amylovora in Montenegro.
PB  - Edizioni Ets, Pisa
T2  - Journal of Plant Pathology
T1  - Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro
EP  - 203
IS  - 1
SP  - 197
VL  - 99
DO  - 10.4454/jpp.v99i1.3853
ER  - 

@article{
author = "Radunović, Dragana and Gavrilović, Veljko and Gašić, Katarina and Paunović, M. and Stojšin, Vera and Grahovac, M.",
year = "2017",
abstract = "In the period from 2012-2015 plant samples with fireblight symptoms were collected from pome fruits and indigenous plant, in main fruitgrowing regions of Montenegro. After succesfull isolation, pathogenicity of the obtained strains was tested by artificial inoculation of immature pear fruits, variety Viljamovka. Hypersensitive reaction was tested on tobacco leaves, variety White Burley. Identification and genetic diversity studies were performed using several molecular techniques on 18 Erwinia amylovora strains originating from quince, pear, apple and hawthorn. Bacterial identity was confirmed by nested PCR in which all studied strains produced the expected amplification fragment of plasmid pEA29. To detect potential genetic variations in E. amylovora population, rep-PCR was conducted. Using REP, ERIC and BOX primers, in all three PCR reactions, differences between studied strains were detected, i.e. pear strains had different genetic profiles from all other studied strains, including reference strain. Genetic variability of selected E. amylovora strains was studied by RAPD-PCR as well. Both of the used random primers, CUGEA-3 and CUGEA-5, showed discriminatory potential by separating genetic profiles of pear strains from all other studied strains, including reference strain. This is the first study of genetic variability of E. amylovora in Montenegro.",
publisher = "Edizioni Ets, Pisa",
journal = "Journal of Plant Pathology",
title = "Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro",
pages = "203-197",
number = "1",
volume = "99",
doi = "10.4454/jpp.v99i1.3853"
}

Radunović, D., Gavrilović, V., Gašić, K., Paunović, M., Stojšin, V.,& Grahovac, M.. (2017). Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro. in Journal of Plant Pathology
Edizioni Ets, Pisa., 99(1), 197-203.
https://doi.org/10.4454/jpp.v99i1.3853

Radunović D, Gavrilović V, Gašić K, Paunović M, Stojšin V, Grahovac M. Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro. in Journal of Plant Pathology. 2017;99(1):197-203.
doi:10.4454/jpp.v99i1.3853 .

Radunović, Dragana, Gavrilović, Veljko, Gašić, Katarina, Paunović, M., Stojšin, Vera, Grahovac, M., "Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro" in Journal of Plant Pathology, 99, no. 1 (2017):197-203,
https://doi.org/10.4454/jpp.v99i1.3853 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Milosević, Drago
AU  - Gašić, Katarina
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/449
AB  - The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia
EP  - 496
IS  - 2
SP  - 487
VL  - 48
DO  - 10.2298/GENSR1602487R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Milosević, Drago and Gašić, Katarina and Dolovac, Nenad and Starović, Mira",
year = "2016",
abstract = "The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia",
pages = "496-487",
number = "2",
volume = "48",
doi = "10.2298/GENSR1602487R"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Milosević, D., Gašić, K., Dolovac, N.,& Starović, M.. (2016). Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 48(2), 487-496.
https://doi.org/10.2298/GENSR1602487R

Ristić D, Vučurović I, Kuzmanović S, Milosević D, Gašić K, Dolovac N, Starović M. Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade. 2016;48(2):487-496.
doi:10.2298/GENSR1602487R .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Milosević, Drago, Gašić, Katarina, Dolovac, Nenad, Starović, Mira, "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia" in Genetika-Belgrade, 48, no. 2 (2016):487-496,
https://doi.org/10.2298/GENSR1602487R . .

TY  - CONF
AU  - Ivanović, M.
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/447
AB  - Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia
EP  - 719
SP  - 715
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.122
ER  - 

@conference{
author = "Ivanović, M. and Gašić, Katarina and Prokić, Anđelka and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2016",
abstract = "Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia",
pages = "719-715",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.122"
}

Ivanović, M., Gašić, K., Prokić, A., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2016). Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 715-719.
https://doi.org/10.17660/ActaHortic.2016.1139.122

Ivanović M, Gašić K, Prokić A, Kuzmanović N, Zlatković N, Obradović A. Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing. 2016;1139:715-719.
doi:10.17660/ActaHortic.2016.1139.122 .

Ivanović, M., Gašić, Katarina, Prokić, Anđelka, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia" in III Balkan Symposium on Fruit Growing, 1139 (2016):715-719,
https://doi.org/10.17660/ActaHortic.2016.1139.122 . .

TY  - CONF
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Đorđević, M.
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Zecević, B.
AU  - Obradović, Aleksa
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/427
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - VI Balkan Symposium on Vegetables and Potatoes
T1  - Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot
EP  - 150
SP  - 147
VL  - 1142
DO  - 10.17660/ActaHortic.2016.1142.23
ER  - 

@conference{
author = "Šević, Milan and Gašić, Katarina and Đorđević, M. and Ignjatov, Maja and Mijatović, Mirjana and Zecević, B. and Obradović, Aleksa",
year = "2016",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "VI Balkan Symposium on Vegetables and Potatoes",
title = "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot",
pages = "150-147",
volume = "1142",
doi = "10.17660/ActaHortic.2016.1142.23"
}

Šević, M., Gašić, K., Đorđević, M., Ignjatov, M., Mijatović, M., Zecević, B.,& Obradović, A.. (2016). Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in VI Balkan Symposium on Vegetables and Potatoes
Int Soc Horticultural Science, Leuven 1., 1142, 147-150.
https://doi.org/10.17660/ActaHortic.2016.1142.23

Šević M, Gašić K, Đorđević M, Ignjatov M, Mijatović M, Zecević B, Obradović A. Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in VI Balkan Symposium on Vegetables and Potatoes. 2016;1142:147-150.
doi:10.17660/ActaHortic.2016.1142.23 .

Šević, Milan, Gašić, Katarina, Đorđević, M., Ignjatov, Maja, Mijatović, Mirjana, Zecević, B., Obradović, Aleksa, "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot" in VI Balkan Symposium on Vegetables and Potatoes, 1142 (2016):147-150,
https://doi.org/10.17660/ActaHortic.2016.1142.23 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/376
AB  - During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014.
AB  - U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial fruit blotch of watermelon in Serbia
T1  - Bakteriozna mrljavost plodova lubenice u Srbiji
EP  - 271
IS  - 3
SP  - 265
VL  - 43
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Anđelka and Kuzmanović, Nemanja and Gašić, Katarina and Šević, Milan and Ivanović, Milan and Obradović, Aleksa",
year = "2015",
abstract = "During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014., U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial fruit blotch of watermelon in Serbia, Bakteriozna mrljavost plodova lubenice u Srbiji",
pages = "271-265",
number = "3",
volume = "43"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Šević, M., Ivanović, M.,& Obradović, A.. (2015). Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(3), 265-271.

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Šević M, Ivanović M, Obradović A. Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar. 2015;43(3):265-271..

Zlatković, Nevena, Prokić, Anđelka, Kuzmanović, Nemanja, Gašić, Katarina, Šević, Milan, Ivanović, Milan, Obradović, Aleksa, "Bacterial fruit blotch of watermelon in Serbia" in Biljni lekar, 43, no. 3 (2015):265-271.

TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/375
AB  - Bacterial diseases are very common in potato crops, especially when weather conditions are favourable for the emergence and spread of infection. Bacteria belonging to the Pectobacterium and Dickeya genera, the causal agents of the blackleg and tuber soft rot of potato, are economically important pathogens that affect potato production worldwide, as well as in our country. According to recent classification, pectolytic bacteria, the pathogens of potato, have been differentiated as Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum, P. carotovorum subsp. brasiliense, P. wasabiae and several Dickeya spp. The disease occurs on potato plants and tubers in the field, or during potato storage or transportation, thereby reducing yield and quality. The aim of this paper is to point to the distribution and economic importance of the blackleg and tuber soft rot of potato, as well as the characteristics of the pathogens. Information about symptoms and epidemiology of the disease are important for an accurate disease diagnosis and pathogen identification, contributing to the timely and successful protection strategy.
AB  - Oboljenja bakteriozne prirode veoma su česta u usevima krompira, posebno kada vremenski uslovi pogoduju nastanku i širenju infekcije. Među ekonomski značajnim bakterijama koje ugrožavaju proizvodnju krompira u svetu i u našoj zemlji, izdvajaju se prouzrokovači crne truleži prizemnog dela stabla ('crna noga') i vlažne truleži krtola krompira, pripadnici rodova Pectobacterium i Dickeya. Prema najnovijoj klasifikaciji, pektolitičke bakterije, patogeni krompira, diferencirane su u vrste Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum, P. carotovorum subsp. brasiliense, P. wasabiae i nekoliko Dickeya spp. Bolest se može pojaviti na biljkama i krtolama u polju ili na krtolama tokom transporta i u skladištu, umanjujući prinos useva i kvalitet krtola. Cilj ovog rada je da se prikaže rasprostranjenost i ekonomski značaj crne truleži prizemnog dela stabla i vlažne truleži krtola krompira, kao i osnovne karakteristike patogena. Poznavanje simptomatologije i epidemiologije oboljenja je od posebnog značaja za pravilnu dijagnozu oboljenja i identifikaciju patogena, što doprinosi pravovremenoj i uspešnoj zaštiti.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of potato: Potato blackleg and tuber soft rot
T1  - Bakterioze krompira - crna trulež prizemnog dela stabla ('crna noga') i vlažna trulež krtola krompira
EP  - 540
IS  - 6
SP  - 529
VL  - 43
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "Bacterial diseases are very common in potato crops, especially when weather conditions are favourable for the emergence and spread of infection. Bacteria belonging to the Pectobacterium and Dickeya genera, the causal agents of the blackleg and tuber soft rot of potato, are economically important pathogens that affect potato production worldwide, as well as in our country. According to recent classification, pectolytic bacteria, the pathogens of potato, have been differentiated as Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum, P. carotovorum subsp. brasiliense, P. wasabiae and several Dickeya spp. The disease occurs on potato plants and tubers in the field, or during potato storage or transportation, thereby reducing yield and quality. The aim of this paper is to point to the distribution and economic importance of the blackleg and tuber soft rot of potato, as well as the characteristics of the pathogens. Information about symptoms and epidemiology of the disease are important for an accurate disease diagnosis and pathogen identification, contributing to the timely and successful protection strategy., Oboljenja bakteriozne prirode veoma su česta u usevima krompira, posebno kada vremenski uslovi pogoduju nastanku i širenju infekcije. Među ekonomski značajnim bakterijama koje ugrožavaju proizvodnju krompira u svetu i u našoj zemlji, izdvajaju se prouzrokovači crne truleži prizemnog dela stabla ('crna noga') i vlažne truleži krtola krompira, pripadnici rodova Pectobacterium i Dickeya. Prema najnovijoj klasifikaciji, pektolitičke bakterije, patogeni krompira, diferencirane su u vrste Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum, P. carotovorum subsp. brasiliense, P. wasabiae i nekoliko Dickeya spp. Bolest se može pojaviti na biljkama i krtolama u polju ili na krtolama tokom transporta i u skladištu, umanjujući prinos useva i kvalitet krtola. Cilj ovog rada je da se prikaže rasprostranjenost i ekonomski značaj crne truleži prizemnog dela stabla i vlažne truleži krtola krompira, kao i osnovne karakteristike patogena. Poznavanje simptomatologije i epidemiologije oboljenja je od posebnog značaja za pravilnu dijagnozu oboljenja i identifikaciju patogena, što doprinosi pravovremenoj i uspešnoj zaštiti.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of potato: Potato blackleg and tuber soft rot, Bakterioze krompira - crna trulež prizemnog dela stabla ('crna noga') i vlažna trulež krtola krompira",
pages = "540-529",
number = "6",
volume = "43"
}

Gašić, K.,& Obradović, A.. (2015). Bacterial diseases of potato: Potato blackleg and tuber soft rot. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(6), 529-540.

Gašić K, Obradović A. Bacterial diseases of potato: Potato blackleg and tuber soft rot. in Biljni lekar. 2015;43(6):529-540..

Gašić, Katarina, Obradović, Aleksa, "Bacterial diseases of potato: Potato blackleg and tuber soft rot" in Biljni lekar, 43, no. 6 (2015):529-540.

TY  - JOUR
AU  - Radunović, Dragana
AU  - Gavrilović, Veljko
AU  - Gašić, Katarina
AU  - Krstić, Marija
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/394
AB  - Recent studies of Erwinia amylovora in Montenegro, conducted from 2012 to 2014, indicated that the bacterium was widespread in the northern, continental part of the country, where the most important fruit-growing regions are situated. The presence of the bacterium was confirmed on quince, pear, apple, medlar and hawthorn. Pathogenic, cultural and biochemical characteristics of E. amylovora strains sampled from pome fruit species and indigenous flora in Montenegro had been studied previously. In the present study, serological tests were used for identification of E. amylovora strains originating from pome fruit trees and indigenous plants. Monitoring of E. amylovora and collection of samples with symptoms of bacterial fire blight from different hosts and locations were performed in Montenegro from 2012 to 2014. Isolation of the bacterium on nutrient medium produced a large number of isolates, whose pathogenicity was confirmed on immature pear fruits. Twenty-seven strains of the bacterium, originating from three pome fruit species (quince, pear and apple) and one indigenous species (hawthorn) were selected for serological analyses. Two applied serological methods, ELISA and IF test, enabled rapid detection of the bacterium and simultaneous examination of a large number of samples over a short period of time. Serological analyses showed high homogeneity in antigenic structure of the studied E. amylovora strains sampled from quince, pear, apple and hawthorn from nine locations in Montenegro.
AB  - Novija proučavanja Erwinia amylovora u Crnoj Gori, sprovedena u periodu od 2012 do 2014. godine, pokazuju da je ova bakterija široko rasprostranjena u severnom, kontinentalnom delu zemlje, gde se nalaze i najznačajniji voćarski regioni. Prisustvo bakterije potvrđeno je na dunji, krušci, jabuci, mušmuli i glogu. U prethodnim istraživanjima proučene su patogene, odgajivačke i biohemijske odlike sojeva E. amylovora poreklom sa jabučastih voćnih vrsta i biljaka spontane flore u Crnoj Gori. U ovom radu primenjeni su serološki testovi u identifikaciji sojeva E. amylovora poreklom sa jabučastih voćaka i biljaka spontane flore. Monitoring E. amylovora i sakupljanje uzoraka sa simptomima bakteriozne plamenjače izvršeno je u periodu od 2012 do 2014. godine, sa različitih domaćina i lokaliteta u Crnoj Gori. Izolacijom bakterije na hranljive podloge dobijen je veći broj izolata, čija je patogenost potvrđena na zelenim plodovima kruške. Za serološke analize odabrano je 27 sojeva ove bakterije, poreklom sa tri jabučaste voćne vrste (dunja, kruška i jabuka) i jedne vrste iz spontane flore (glog). Primenjene su dve serološke metode: ELISA i IF test, koje su omogućile brzu detekciju bakterije i istovremeno ispitivanje velikog broja uzoraka za kratko vreme. Serološkim analizama utvrđena je visoka homogenost u antigenoj strukturi proučavanih sojeva E. amylovora poreklom sa dunje, kruške, jabuke i gloga, iz 9 lokaliteta u Crnoj Gori.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Monitoring of Erwinia amylovora in Montenegro
T1  - Monitoring Erwinia amylovora u Crnoj Gori
EP  - 185
IS  - 3
SP  - 179
VL  - 30
DO  - 10.2298/pif1503179r
ER  - 

@article{
author = "Radunović, Dragana and Gavrilović, Veljko and Gašić, Katarina and Krstić, Marija",
year = "2015",
abstract = "Recent studies of Erwinia amylovora in Montenegro, conducted from 2012 to 2014, indicated that the bacterium was widespread in the northern, continental part of the country, where the most important fruit-growing regions are situated. The presence of the bacterium was confirmed on quince, pear, apple, medlar and hawthorn. Pathogenic, cultural and biochemical characteristics of E. amylovora strains sampled from pome fruit species and indigenous flora in Montenegro had been studied previously. In the present study, serological tests were used for identification of E. amylovora strains originating from pome fruit trees and indigenous plants. Monitoring of E. amylovora and collection of samples with symptoms of bacterial fire blight from different hosts and locations were performed in Montenegro from 2012 to 2014. Isolation of the bacterium on nutrient medium produced a large number of isolates, whose pathogenicity was confirmed on immature pear fruits. Twenty-seven strains of the bacterium, originating from three pome fruit species (quince, pear and apple) and one indigenous species (hawthorn) were selected for serological analyses. Two applied serological methods, ELISA and IF test, enabled rapid detection of the bacterium and simultaneous examination of a large number of samples over a short period of time. Serological analyses showed high homogeneity in antigenic structure of the studied E. amylovora strains sampled from quince, pear, apple and hawthorn from nine locations in Montenegro., Novija proučavanja Erwinia amylovora u Crnoj Gori, sprovedena u periodu od 2012 do 2014. godine, pokazuju da je ova bakterija široko rasprostranjena u severnom, kontinentalnom delu zemlje, gde se nalaze i najznačajniji voćarski regioni. Prisustvo bakterije potvrđeno je na dunji, krušci, jabuci, mušmuli i glogu. U prethodnim istraživanjima proučene su patogene, odgajivačke i biohemijske odlike sojeva E. amylovora poreklom sa jabučastih voćnih vrsta i biljaka spontane flore u Crnoj Gori. U ovom radu primenjeni su serološki testovi u identifikaciji sojeva E. amylovora poreklom sa jabučastih voćaka i biljaka spontane flore. Monitoring E. amylovora i sakupljanje uzoraka sa simptomima bakteriozne plamenjače izvršeno je u periodu od 2012 do 2014. godine, sa različitih domaćina i lokaliteta u Crnoj Gori. Izolacijom bakterije na hranljive podloge dobijen je veći broj izolata, čija je patogenost potvrđena na zelenim plodovima kruške. Za serološke analize odabrano je 27 sojeva ove bakterije, poreklom sa tri jabučaste voćne vrste (dunja, kruška i jabuka) i jedne vrste iz spontane flore (glog). Primenjene su dve serološke metode: ELISA i IF test, koje su omogućile brzu detekciju bakterije i istovremeno ispitivanje velikog broja uzoraka za kratko vreme. Serološkim analizama utvrđena je visoka homogenost u antigenoj strukturi proučavanih sojeva E. amylovora poreklom sa dunje, kruške, jabuke i gloga, iz 9 lokaliteta u Crnoj Gori.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Monitoring of Erwinia amylovora in Montenegro, Monitoring Erwinia amylovora u Crnoj Gori",
pages = "185-179",
number = "3",
volume = "30",
doi = "10.2298/pif1503179r"
}

Radunović, D., Gavrilović, V., Gašić, K.,& Krstić, M.. (2015). Monitoring of Erwinia amylovora in Montenegro. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 30(3), 179-185.
https://doi.org/10.2298/pif1503179r

Radunović D, Gavrilović V, Gašić K, Krstić M. Monitoring of Erwinia amylovora in Montenegro. in Pesticidi i fitomedicina. 2015;30(3):179-185.
doi:10.2298/pif1503179r .

Radunović, Dragana, Gavrilović, Veljko, Gašić, Katarina, Krstić, Marija, "Monitoring of Erwinia amylovora in Montenegro" in Pesticidi i fitomedicina, 30, no. 3 (2015):179-185,
https://doi.org/10.2298/pif1503179r . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/381
AB  - Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.
PB  - Amer Soc Microbiology, Washington
T2  - Microbiology Resource Announcements
T1  - Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330
IS  - 2
VL  - 3
DO  - 10.1128/genomeA.00331-15
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Anđelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Microbiology Resource Announcements",
title = "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330",
number = "2",
volume = "3",
doi = "10.1128/genomeA.00331-15"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements
Amer Soc Microbiology, Washington., 3(2).
https://doi.org/10.1128/genomeA.00331-15

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements. 2015;3(2).
doi:10.1128/genomeA.00331-15 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Anđelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330" in Microbiology Resource Announcements, 3, no. 2 (2015),
https://doi.org/10.1128/genomeA.00331-15 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/384
AB  - During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease. Out of 14 strains isolated from raspberry tumors, 12 were identified as tumorigenic Rhizobium rhizogenes, one belonged to Agrobacterium tumefaciens genomic species G8, while the remaining strain formed a separate phylogenetic lineage within A. tumefaciens species complex, different from all known genomic species. All strains investigated harbored nopaline-type of Ti plasmid and showed identical pathogenic properties by inoculating several test plants. However, they were divided into two genetic groups based on PCR-RFLP analysis of Ti plasmid virA-virB2 region. Furthermore, total of nine unique ERIC-PCR profiles were identified among the strains studied. Although strains of R. rhizogenes exhibited similar ERIC-PCR profiles, they were differentiated into six distinct genetic groups. Based on the fact that some genetic groups were composed of strains originating from different geographic areas, it can be assumed that they have a common origin and were probably disseminated by movement of infected plant material.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia
EP  - 713
IS  - 4
SP  - 701
VL  - 142
DO  - 10.1007/s10658-015-0645-4
ER  - 

@article{
author = "Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease. Out of 14 strains isolated from raspberry tumors, 12 were identified as tumorigenic Rhizobium rhizogenes, one belonged to Agrobacterium tumefaciens genomic species G8, while the remaining strain formed a separate phylogenetic lineage within A. tumefaciens species complex, different from all known genomic species. All strains investigated harbored nopaline-type of Ti plasmid and showed identical pathogenic properties by inoculating several test plants. However, they were divided into two genetic groups based on PCR-RFLP analysis of Ti plasmid virA-virB2 region. Furthermore, total of nine unique ERIC-PCR profiles were identified among the strains studied. Although strains of R. rhizogenes exhibited similar ERIC-PCR profiles, they were differentiated into six distinct genetic groups. Based on the fact that some genetic groups were composed of strains originating from different geographic areas, it can be assumed that they have a common origin and were probably disseminated by movement of infected plant material.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia",
pages = "713-701",
number = "4",
volume = "142",
doi = "10.1007/s10658-015-0645-4"
}

Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 142(4), 701-713.
https://doi.org/10.1007/s10658-015-0645-4

Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia. in European Journal of Plant Pathology. 2015;142(4):701-713.
doi:10.1007/s10658-015-0645-4 .

Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia" in European Journal of Plant Pathology, 142, no. 4 (2015):701-713,
https://doi.org/10.1007/s10658-015-0645-4 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/362
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 

@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}

Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G

Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .

Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/332
AB  - Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control.
AB  - Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - New strategies for pepper and tomato bacterial spot control
T1  - Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti
EP  - 307
IS  - 4
SP  - 296
VL  - 42
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Obradović, Aleksa",
year = "2014",
abstract = "Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control., Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "New strategies for pepper and tomato bacterial spot control, Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti",
pages = "307-296",
number = "4",
volume = "42"
}

Šević, M., Gašić, K.,& Obradović, A.. (2014). New strategies for pepper and tomato bacterial spot control. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 42(4), 296-307.

Šević M, Gašić K, Obradović A. New strategies for pepper and tomato bacterial spot control. in Biljni lekar. 2014;42(4):296-307..

Šević, Milan, Gašić, Katarina, Obradović, Aleksa, "New strategies for pepper and tomato bacterial spot control" in Biljni lekar, 42, no. 4 (2014):296-307.