TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/588
AB  - Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.
PB  - Springer Nature
T2  - European Journal of Plant Pathology
T1  - Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential
EP  - 221
SP  - 201
VL  - 156
DO  - 10.1007/s10658-019-01878-w
ER  - 

@article{
author = "Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Mitrović, Milana and Marinković, Slavica and Toševski, Ivo and Cvrković, Tatjana",
year = "2020",
abstract = "Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.",
publisher = "Springer Nature",
journal = "European Journal of Plant Pathology",
title = "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential",
pages = "221-201",
volume = "156",
doi = "10.1007/s10658-019-01878-w"
}

Jakovljević, M., Jović, J., Krstić, O., Mitrović, M., Marinković, S., Toševski, I.,& Cvrković, T.. (2020). Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology
Springer Nature., 156, 201-221.
https://doi.org/10.1007/s10658-019-01878-w

Jakovljević M, Jović J, Krstić O, Mitrović M, Marinković S, Toševski I, Cvrković T. Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology. 2020;156:201-221.
doi:10.1007/s10658-019-01878-w .

Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Mitrović, Milana, Marinković, Slavica, Toševski, Ivo, Cvrković, Tatjana, "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential" in European Journal of Plant Pathology, 156 (2020):201-221,
https://doi.org/10.1007/s10658-019-01878-w . .

TY  - JOUR
AU  - Sedlarević-Zorić, Ana
AU  - Morina, Filis
AU  - Toševski, Ivo
AU  - Tosti, Tomislav
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/569
AB  - Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.
PB  - Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales
T2  - Plant Physiology and Biochemistry
T1  - Resource allocation in response to herbivory and gall formation in Linaria vulgaris
EP  - 232
SP  - 224
VL  - 135
DO  - 10.1016/j.plaphy.2018.11.032
ER  - 

@article{
author = "Sedlarević-Zorić, Ana and Morina, Filis and Toševski, Ivo and Tosti, Tomislav and Jović, Jelena and Krstić, Oliver and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.",
publisher = "Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales",
journal = "Plant Physiology and Biochemistry",
title = "Resource allocation in response to herbivory and gall formation in Linaria vulgaris",
pages = "232-224",
volume = "135",
doi = "10.1016/j.plaphy.2018.11.032"
}

Sedlarević-Zorić, A., Morina, F., Toševski, I., Tosti, T., Jović, J., Krstić, O.,& Veljović-Jovanović, S.. (2019). Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry
Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales., 135, 224-232.
https://doi.org/10.1016/j.plaphy.2018.11.032

Sedlarević-Zorić A, Morina F, Toševski I, Tosti T, Jović J, Krstić O, Veljović-Jovanović S. Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry. 2019;135:224-232.
doi:10.1016/j.plaphy.2018.11.032 .

Sedlarević-Zorić, Ana, Morina, Filis, Toševski, Ivo, Tosti, Tomislav, Jović, Jelena, Krstić, Oliver, Veljović-Jovanović, Sonja, "Resource allocation in response to herbivory and gall formation in Linaria vulgaris" in Plant Physiology and Biochemistry, 135 (2019):224-232,
https://doi.org/10.1016/j.plaphy.2018.11.032 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/565
AB  - Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia
EP  - 195
IS  - 1
SP  - 183
VL  - 153
DO  - 10.1007/s10658-018-1553-1
ER  - 

@article{
author = "Kosovac, Andrea and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2019",
abstract = "Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia",
pages = "195-183",
number = "1",
volume = "153",
doi = "10.1007/s10658-018-1553-1"
}

Kosovac, A., Jakovljević, M., Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2019). Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 153(1), 183-195.
https://doi.org/10.1007/s10658-018-1553-1

Kosovac A, Jakovljević M, Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology. 2019;153(1):183-195.
doi:10.1007/s10658-018-1553-1 .

Kosovac, Andrea, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia" in European Journal of Plant Pathology, 153, no. 1 (2019):183-195,
https://doi.org/10.1007/s10658-018-1553-1 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/547
AB  - The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).
PB  - Public Library of Science (PLoS)
T2  - PlOS One
T1  - Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation
IS  - 5
VL  - 13
DO  - 10.1371/journal.pone.0196969
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).",
publisher = "Public Library of Science (PLoS)",
journal = "PlOS One",
title = "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation",
number = "5",
volume = "13",
doi = "10.1371/journal.pone.0196969"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2018). Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One
Public Library of Science (PLoS)., 13(5).
https://doi.org/10.1371/journal.pone.0196969

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One. 2018;13(5).
doi:10.1371/journal.pone.0196969 .

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation" in PlOS One, 13, no. 5 (2018),
https://doi.org/10.1371/journal.pone.0196969 . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/550
AB  - The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions
EP  - 64
IS  - 1
SP  - 47
VL  - 172
DO  - 10.1111/aab.12400
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Radonjić, Sanja and Hrnčić, Snježana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions",
pages = "64-47",
number = "1",
volume = "172",
doi = "10.1111/aab.12400"
}

Krstić, O., Cvrković, T., Mitrović, M., Radonjić, S., Hrnčić, S., Toševski, I.,& Jović, J.. (2018). Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology
Wiley, Hoboken., 172(1), 47-64.
https://doi.org/10.1111/aab.12400

Krstić O, Cvrković T, Mitrović M, Radonjić S, Hrnčić S, Toševski I, Jović J. Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology. 2018;172(1):47-64.
doi:10.1111/aab.12400 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Radonjić, Sanja, Hrnčić, Snježana, Toševski, Ivo, Jović, Jelena, "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions" in Annals of Applied Biology, 172, no. 1 (2018):47-64,
https://doi.org/10.1111/aab.12400 . .

TY  - JOUR
AU  - Toševski, Ivo
AU  - Sing, Sharlene
AU  - De Clerck-Floate, Rosemarie
AU  - McClay, A.
AU  - Weaver, D. K.
AU  - Schwarzlander, M.
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Gassmann, Andre
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/549
AB  - Linaria vulgaris, common or yellow toadflax, and Linaria dalmatica, Dalmatian toadflax (Plantaginaceae), are Eurasian perennial forbs invasive throughout temperate North America. These Linaria species have been the targets of classical biological control programmes in Canada and the USA since the 1960s. The first effective toadflax biological control agent, the stem-mining weevil Mecinus janthinus (Coleoptera: Curculionidae) was introduced from Europe in the 1990s. This weevil has become established on L. dalmatica and L. vulgaris in both countries, although it has shown greater success in controlling the former toadflax species. Genetic and ecological studies of native range M. janthinus populations revealed that weevils previously identified as a single species in fact include two cryptic species, now recognised as M. janthinus, associated with yellow toadflax, and the recently confirmed species Mecinus janthiniformis, associated with Dalmatian toadflax. The results of a comprehensive study characterising haplotype identities, distributions and frequencies within M. janthinus s.l. native range source populations were compared to those populations currently established in the USA and Canada. The presence of both Mecinus species in North America was confirmed, and revealed with a few exceptions a high and consistent level of host fidelity throughout the adopted and native ranges. Genetic analysis based on mitochondrial cytochrome oxidase subunit II gene (mtCOII) defined the origin and records the subsequent North American establishment, by haplotype, of the European founder populations of M. janthinus (northern Switzerland and southern Germany) and M. janthiniformis (southern Macedonia), and provided population genetic indices for the studied populations. This analysis together with existing North American shipment receipt, release and rearing records elucidates probable redistribution routes and sources of both weevil species from initially released and established adopted range populations.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America
EP  - 34
IS  - 1
SP  - 16
VL  - 173
DO  - 10.1111/aab.12430
ER  - 

@article{
author = "Toševski, Ivo and Sing, Sharlene and De Clerck-Floate, Rosemarie and McClay, A. and Weaver, D. K. and Schwarzlander, M. and Krstić, Oliver and Jović, Jelena and Gassmann, Andre",
year = "2018",
abstract = "Linaria vulgaris, common or yellow toadflax, and Linaria dalmatica, Dalmatian toadflax (Plantaginaceae), are Eurasian perennial forbs invasive throughout temperate North America. These Linaria species have been the targets of classical biological control programmes in Canada and the USA since the 1960s. The first effective toadflax biological control agent, the stem-mining weevil Mecinus janthinus (Coleoptera: Curculionidae) was introduced from Europe in the 1990s. This weevil has become established on L. dalmatica and L. vulgaris in both countries, although it has shown greater success in controlling the former toadflax species. Genetic and ecological studies of native range M. janthinus populations revealed that weevils previously identified as a single species in fact include two cryptic species, now recognised as M. janthinus, associated with yellow toadflax, and the recently confirmed species Mecinus janthiniformis, associated with Dalmatian toadflax. The results of a comprehensive study characterising haplotype identities, distributions and frequencies within M. janthinus s.l. native range source populations were compared to those populations currently established in the USA and Canada. The presence of both Mecinus species in North America was confirmed, and revealed with a few exceptions a high and consistent level of host fidelity throughout the adopted and native ranges. Genetic analysis based on mitochondrial cytochrome oxidase subunit II gene (mtCOII) defined the origin and records the subsequent North American establishment, by haplotype, of the European founder populations of M. janthinus (northern Switzerland and southern Germany) and M. janthiniformis (southern Macedonia), and provided population genetic indices for the studied populations. This analysis together with existing North American shipment receipt, release and rearing records elucidates probable redistribution routes and sources of both weevil species from initially released and established adopted range populations.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America",
pages = "34-16",
number = "1",
volume = "173",
doi = "10.1111/aab.12430"
}

Toševski, I., Sing, S., De Clerck-Floate, R., McClay, A., Weaver, D. K., Schwarzlander, M., Krstić, O., Jović, J.,& Gassmann, A.. (2018). Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America. in Annals of Applied Biology
Wiley, Hoboken., 173(1), 16-34.
https://doi.org/10.1111/aab.12430

Toševski I, Sing S, De Clerck-Floate R, McClay A, Weaver DK, Schwarzlander M, Krstić O, Jović J, Gassmann A. Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America. in Annals of Applied Biology. 2018;173(1):16-34.
doi:10.1111/aab.12430 .

Toševski, Ivo, Sing, Sharlene, De Clerck-Floate, Rosemarie, McClay, A., Weaver, D. K., Schwarzlander, M., Krstić, Oliver, Jović, Jelena, Gassmann, Andre, "Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America" in Annals of Applied Biology, 173, no. 1 (2018):16-34,
https://doi.org/10.1111/aab.12430 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Sayadi, Ahmed
AU  - Đorđević, Mirko
AU  - Jović, Jelena
AU  - Savković, Uroš
AU  - Arnqvist, Goran
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/471
AB  - Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for  gt 190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.
PB  - Wiley, Hoboken
T2  - Evolution
T1  - Divergent evolution of life span associated with mitochondrial DNA evolution
EP  - 166
IS  - 1
SP  - 160
VL  - 71
DO  - 10.1111/evo.13102
ER  - 

@article{
author = "Stojković, Biljana and Sayadi, Ahmed and Đorđević, Mirko and Jović, Jelena and Savković, Uroš and Arnqvist, Goran",
year = "2017",
abstract = "Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for  gt 190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.",
publisher = "Wiley, Hoboken",
journal = "Evolution",
title = "Divergent evolution of life span associated with mitochondrial DNA evolution",
pages = "166-160",
number = "1",
volume = "71",
doi = "10.1111/evo.13102"
}

Stojković, B., Sayadi, A., Đorđević, M., Jović, J., Savković, U.,& Arnqvist, G.. (2017). Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution
Wiley, Hoboken., 71(1), 160-166.
https://doi.org/10.1111/evo.13102

Stojković B, Sayadi A, Đorđević M, Jović J, Savković U, Arnqvist G. Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution. 2017;71(1):160-166.
doi:10.1111/evo.13102 .

Stojković, Biljana, Sayadi, Ahmed, Đorđević, Mirko, Jović, Jelena, Savković, Uroš, Arnqvist, Goran, "Divergent evolution of life span associated with mitochondrial DNA evolution" in Evolution, 71, no. 1 (2017):160-166,
https://doi.org/10.1111/evo.13102 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Khan, Mohamed F. R.
AU  - Secor, Gary A.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/475
AB  - Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides
EP  - 910
IS  - 4
SP  - 895
VL  - 149
DO  - 10.1007/s10658-017-1239-0
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Khan, Mohamed F. R. and Secor, Gary A.",
year = "2017",
abstract = "Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides",
pages = "910-895",
number = "4",
volume = "149",
doi = "10.1007/s10658-017-1239-0"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J., Toševski, I., Khan, M. F. R.,& Secor, G. A.. (2017). Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology
Springer, Dordrecht., 149(4), 895-910.
https://doi.org/10.1007/s10658-017-1239-0

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I, Khan MFR, Secor GA. Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology. 2017;149(4):895-910.
doi:10.1007/s10658-017-1239-0 .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Khan, Mohamed F. R., Secor, Gary A., "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides" in European Journal of Plant Pathology, 149, no. 4 (2017):895-910,
https://doi.org/10.1007/s10658-017-1239-0 . .

TY  - JOUR
AU  - Hamidović, Saud
AU  - Teodorović, Smilja
AU  - Lalević, Blažo
AU  - Jovicic-Petrović, Jelena
AU  - Jović, Jelena
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/429
AB  - Coal and lignite play a major energy supply role in many European countries, including Bosnia and Herzegovina. Yet mining activities are a heavy source of ecosystem contamination, posing significant environmental threats. The primary goal of this study was to isolate and identify autochthonous lignite mine spoil bacteria and evaluate their potential in bioremediation of these polluted soils. Two Bacillus species, Bacillus simplex and a Bacillus cereus group member, were identified using conventional, molecular, and bioinformatics approaches. This represents, to our knowledge, the first microbial characterization of mine overburden in Bosnia and Herzegovina. A co-inoculum of autochthonous bacterial populations was used to treat unvegetated as well as oat- and lettuce-vegetated lignite overburden samples. Our results illustrate the potential of recovered native species to enrich soil fertility and productivity through plant growth promotion.
PB  - Hard, Olsztyn 5
T2  - Polish Journal of Environmental Studies
T1  - Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study
EP  - 119
IS  - 1
SP  - 113
VL  - 25
DO  - 10.15244/pjoes/59465
ER  - 

@article{
author = "Hamidović, Saud and Teodorović, Smilja and Lalević, Blažo and Jovicic-Petrović, Jelena and Jović, Jelena and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "Coal and lignite play a major energy supply role in many European countries, including Bosnia and Herzegovina. Yet mining activities are a heavy source of ecosystem contamination, posing significant environmental threats. The primary goal of this study was to isolate and identify autochthonous lignite mine spoil bacteria and evaluate their potential in bioremediation of these polluted soils. Two Bacillus species, Bacillus simplex and a Bacillus cereus group member, were identified using conventional, molecular, and bioinformatics approaches. This represents, to our knowledge, the first microbial characterization of mine overburden in Bosnia and Herzegovina. A co-inoculum of autochthonous bacterial populations was used to treat unvegetated as well as oat- and lettuce-vegetated lignite overburden samples. Our results illustrate the potential of recovered native species to enrich soil fertility and productivity through plant growth promotion.",
publisher = "Hard, Olsztyn 5",
journal = "Polish Journal of Environmental Studies",
title = "Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study",
pages = "119-113",
number = "1",
volume = "25",
doi = "10.15244/pjoes/59465"
}

Hamidović, S., Teodorović, S., Lalević, B., Jovicic-Petrović, J., Jović, J., Kiković, D.,& Raičević, V.. (2016). Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study. in Polish Journal of Environmental Studies
Hard, Olsztyn 5., 25(1), 113-119.
https://doi.org/10.15244/pjoes/59465

Hamidović S, Teodorović S, Lalević B, Jovicic-Petrović J, Jović J, Kiković D, Raičević V. Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study. in Polish Journal of Environmental Studies. 2016;25(1):113-119.
doi:10.15244/pjoes/59465 .

Hamidović, Saud, Teodorović, Smilja, Lalević, Blažo, Jovicic-Petrović, Jelena, Jović, Jelena, Kiković, Dragan, Raičević, Vera, "Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study" in Polish Journal of Environmental Studies, 25, no. 1 (2016):113-119,
https://doi.org/10.15244/pjoes/59465 . .

TY  - JOUR
AU  - Stepanović, Svetlana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/445
AB  - Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.
PB  - Wiley, Hoboken
T2  - Insect Science
T1  - Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification
EP  - 648
IS  - 4
SP  - 638
VL  - 23
DO  - 10.1111/1744-7917.12212
ER  - 

@article{
author = "Stepanović, Svetlana and Kosovac, Andrea and Krstić, Oliver and Jović, Jelena and Toševski, Ivo",
year = "2016",
abstract = "Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.",
publisher = "Wiley, Hoboken",
journal = "Insect Science",
title = "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification",
pages = "648-638",
number = "4",
volume = "23",
doi = "10.1111/1744-7917.12212"
}

Stepanović, S., Kosovac, A., Krstić, O., Jović, J.,& Toševski, I.. (2016). Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science
Wiley, Hoboken., 23(4), 638-648.
https://doi.org/10.1111/1744-7917.12212

Stepanović S, Kosovac A, Krstić O, Jović J, Toševski I. Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science. 2016;23(4):638-648.
doi:10.1111/1744-7917.12212 .

Stepanović, Svetlana, Kosovac, Andrea, Krstić, Oliver, Jović, Jelena, Toševski, Ivo, "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification" in Insect Science, 23, no. 4 (2016):638-648,
https://doi.org/10.1111/1744-7917.12212 . .

TY  - JOUR
AU  - Sedlarević, Ana
AU  - Morina, Filis
AU  - Toševski, Ivo
AU  - Gašić, Uroš
AU  - Natić, Maja
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/443
AB  - Rhinusa pilosa (Gyllenhal) is a highly specific weevil that induces stem galls on the common toadflax Linaria vulgaris Mill. females oviposit the eggs near the apex of a growing shoot. The act of oviposition is accompanied by secretion of an ovipositional fluid, which is considered to be cecidogen, directly involved in gall induction. The remains of cecidogenic fluid were collected from the surface of the oviposition point on the stem. We performed a comparative analysis of the phenolics extracted from cecidogen, the stem and galls of L. vulgaris and adult and larva of R. pilosa by HPLC-DAD. One compound with A (max) at 273, 332 nm (R (t) 30.65 min) was exclusively found in the methanol extract of cecidogen. To further characterize the cecidogen and stem phenolic profiles, we used UHPLC coupled with an OrbiTrap mass analyzer. Among 49 phenolic compounds extracted from both the ovipositional fluid and the plant, protocatechuic acid and two phenolic glycosides were exclusively found in cecidogen: diosmetin-O-acetylrutinoside and an unidentified compound. The unknown compound produced an MS2 base peak at 387 and 327 and 267 m/z base peaks at MS3 and MS4 fragmentation, respectively, and had the molecular formula C32H31O18. The plausible role of phenolic compounds in the induction of gall formation on L. vulgaris is discussed.
PB  - Springer, Dordrecht
T2  - Arthropod-Plant Interactions
T1  - Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)
EP  - 322
IS  - 4
SP  - 311
VL  - 10
DO  - 10.1007/s11829-016-9435-y
ER  - 

@article{
author = "Sedlarević, Ana and Morina, Filis and Toševski, Ivo and Gašić, Uroš and Natić, Maja and Jović, Jelena and Krstić, Oliver and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "Rhinusa pilosa (Gyllenhal) is a highly specific weevil that induces stem galls on the common toadflax Linaria vulgaris Mill. females oviposit the eggs near the apex of a growing shoot. The act of oviposition is accompanied by secretion of an ovipositional fluid, which is considered to be cecidogen, directly involved in gall induction. The remains of cecidogenic fluid were collected from the surface of the oviposition point on the stem. We performed a comparative analysis of the phenolics extracted from cecidogen, the stem and galls of L. vulgaris and adult and larva of R. pilosa by HPLC-DAD. One compound with A (max) at 273, 332 nm (R (t) 30.65 min) was exclusively found in the methanol extract of cecidogen. To further characterize the cecidogen and stem phenolic profiles, we used UHPLC coupled with an OrbiTrap mass analyzer. Among 49 phenolic compounds extracted from both the ovipositional fluid and the plant, protocatechuic acid and two phenolic glycosides were exclusively found in cecidogen: diosmetin-O-acetylrutinoside and an unidentified compound. The unknown compound produced an MS2 base peak at 387 and 327 and 267 m/z base peaks at MS3 and MS4 fragmentation, respectively, and had the molecular formula C32H31O18. The plausible role of phenolic compounds in the induction of gall formation on L. vulgaris is discussed.",
publisher = "Springer, Dordrecht",
journal = "Arthropod-Plant Interactions",
title = "Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)",
pages = "322-311",
number = "4",
volume = "10",
doi = "10.1007/s11829-016-9435-y"
}

Sedlarević, A., Morina, F., Toševski, I., Gašić, U., Natić, M., Jović, J., Krstić, O.,& Veljović-Jovanović, S.. (2016). Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae). in Arthropod-Plant Interactions
Springer, Dordrecht., 10(4), 311-322.
https://doi.org/10.1007/s11829-016-9435-y

Sedlarević A, Morina F, Toševski I, Gašić U, Natić M, Jović J, Krstić O, Veljović-Jovanović S. Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae). in Arthropod-Plant Interactions. 2016;10(4):311-322.
doi:10.1007/s11829-016-9435-y .

Sedlarević, Ana, Morina, Filis, Toševski, Ivo, Gašić, Uroš, Natić, Maja, Jović, Jelena, Krstić, Oliver, Veljović-Jovanović, Sonja, "Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)" in Arthropod-Plant Interactions, 10, no. 4 (2016):311-322,
https://doi.org/10.1007/s11829-016-9435-y . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/428
AB  - A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors
EP  - 630
IS  - 3
SP  - 619
VL  - 144
DO  - 10.1007/s10658-015-0800-y
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Jermini, Mauro and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
abstract = "A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors",
pages = "630-619",
number = "3",
volume = "144",
doi = "10.1007/s10658-015-0800-y"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Jermini, M., Toševski, I.,& Cvrković, T.. (2016). 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology
Springer, Dordrecht., 144(3), 619-630.
https://doi.org/10.1007/s10658-015-0800-y

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Jermini M, Toševski I, Cvrković T. 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology. 2016;144(3):619-630.
doi:10.1007/s10658-015-0800-y .

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Jermini, Mauro, Toševski, Ivo, Cvrković, Tatjana, "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors" in European Journal of Plant Pathology, 144, no. 3 (2016):619-630,
https://doi.org/10.1007/s10658-015-0800-y . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/439
AB  - Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)
EP  - 298
IS  - 2
SP  - 285
VL  - 65
DO  - 10.1111/ppa.12409
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)",
pages = "298-285",
number = "2",
volume = "65",
doi = "10.1111/ppa.12409"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology
Wiley, Hoboken., 65(2), 285-298.
https://doi.org/10.1111/ppa.12409

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology. 2016;65(2):285-298.
doi:10.1111/ppa.12409 .

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)" in Plant Pathology, 65, no. 2 (2016):285-298,
https://doi.org/10.1111/ppa.12409 . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/442
AB  - The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dore, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatiotemporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in epidemiological cycles of phytoplasma-induced plant diseases.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations
EP  - 405
IS  - 3
SP  - 395
VL  - 106
DO  - 10.1017/S0007485316000067
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dore, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatiotemporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in epidemiological cycles of phytoplasma-induced plant diseases.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations",
pages = "405-395",
number = "3",
volume = "106",
doi = "10.1017/S0007485316000067"
}

Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 106(3), 395-405.
https://doi.org/10.1017/S0007485316000067

Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations. in Bulletin of Entomological Research. 2016;106(3):395-405.
doi:10.1017/S0007485316000067 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations" in Bulletin of Entomological Research, 106, no. 3 (2016):395-405,
https://doi.org/10.1017/S0007485316000067 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/430
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards
EP  - 3
IS  - 1
SP  - 1
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards",
pages = "3-1",
number = "1",
volume = "66"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 1-3.

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards. in Mitteilungen Klosterneuburg. 2016;66(1):1-3..

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):1-3.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/431
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data
EP  - 11
IS  - 1
SP  - 9
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Krstić, Oliver and Jakovljević, Miljana and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data",
pages = "11-9",
number = "1",
volume = "66"
}

Kosovac, A., Krstić, O., Jakovljević, M., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 9-11.

Kosovac A, Krstić O, Jakovljević M, Cvrković T, Mitrović M, Toševski I, Jović J. Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg. 2016;66(1):9-11..

Kosovac, Andrea, Krstić, Oliver, Jakovljević, Miljana, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):9-11.

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/432
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia
EP  - 18
IS  - 1
SP  - 17
VL  - 66
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia",
pages = "18-17",
number = "1",
volume = "66"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Toševski, I.,& Cvrković, T.. (2016). Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 17-18.

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Toševski I, Cvrković T. Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg. 2016;66(1):17-18..

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Toševski, Ivo, Cvrković, Tatjana, "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):17-18.

TY  - JOUR
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Mitrović, Milana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/433
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland
EP  - 23
IS  - 1
SP  - 22
VL  - 66
ER  - 

@article{
author = "Trivellone, Valeria and Jermini, Mauro and Jović, Jelena and Cvrković, Tatjana and Jakovljević, Miljana and Kosovac, Andrea and Krstić, Oliver and Toševski, Ivo and Mitrović, Milana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland",
pages = "23-22",
number = "1",
volume = "66"
}

Trivellone, V., Jermini, M., Jović, J., Cvrković, T., Jakovljević, M., Kosovac, A., Krstić, O., Toševski, I.,& Mitrović, M.. (2016). Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 22-23.

Trivellone V, Jermini M, Jović J, Cvrković T, Jakovljević M, Kosovac A, Krstić O, Toševski I, Mitrović M. Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg. 2016;66(1):22-23..

Trivellone, Valeria, Jermini, Mauro, Jović, Jelena, Cvrković, Tatjana, Jakovljević, Miljana, Kosovac, Andrea, Krstić, Oliver, Toševski, Ivo, Mitrović, Milana, "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):22-23.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/434
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe
EP  - 25
IS  - 1
SP  - 24
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe",
pages = "25-24",
number = "1",
volume = "66"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2016). Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 24-25.

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg. 2016;66(1):24-25..

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):24-25.

TY  - JOUR
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/444
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro
EP  - 1775
IS  - 8
SP  - 1775
VL  - 100
DO  - 10.1094/PDIS-02-16-0180-PDN
ER  - 

@article{
author = "Radonjić, Sanja and Hrnčić, Snježana and Kosovac, Andrea and Krstić, Oliver and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro",
pages = "1775-1775",
number = "8",
volume = "100",
doi = "10.1094/PDIS-02-16-0180-PDN"
}

Radonjić, S., Hrnčić, S., Kosovac, A., Krstić, O., Mitrović, M., Jović, J.,& Toševski, I.. (2016). First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(8), 1775-1775.
https://doi.org/10.1094/PDIS-02-16-0180-PDN

Radonjić S, Hrnčić S, Kosovac A, Krstić O, Mitrović M, Jović J, Toševski I. First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro. in Plant Disease. 2016;100(8):1775-1775.
doi:10.1094/PDIS-02-16-0180-PDN .

Radonjić, Sanja, Hrnčić, Snježana, Kosovac, Andrea, Krstić, Oliver, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro" in Plant Disease, 100, no. 8 (2016):1775-1775,
https://doi.org/10.1094/PDIS-02-16-0180-PDN . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Milosavljević, Anja
AU  - Bosković, J.
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/455
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia
EP  - 221
IS  - 1
SP  - 221
VL  - 100
DO  - 10.1094/PDIS-02-15-0209-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Pfaf-Dolovac, Erika and Milosavljević, Anja and Bosković, J. and Jović, Jelena and Mitrović, Milana and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia",
pages = "221-221",
number = "1",
volume = "100",
doi = "10.1094/PDIS-02-15-0209-PDN"
}

Trkulja, N., Pfaf-Dolovac, E., Milosavljević, A., Bosković, J., Jović, J., Mitrović, M.,& Toševski, I.. (2016). First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 221-221.
https://doi.org/10.1094/PDIS-02-15-0209-PDN

Trkulja N, Pfaf-Dolovac E, Milosavljević A, Bosković J, Jović J, Mitrović M, Toševski I. First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease. 2016;100(1):221-221.
doi:10.1094/PDIS-02-15-0209-PDN .

Trkulja, Nenad, Pfaf-Dolovac, Erika, Milosavljević, Anja, Bosković, J., Jović, Jelena, Mitrović, Milana, Toševski, Ivo, "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia" in Plant Disease, 100, no. 1 (2016):221-221,
https://doi.org/10.1094/PDIS-02-15-0209-PDN . .

TY  - JOUR
AU  - Atanasova, Biljana
AU  - Jakovljević, Miljana
AU  - Spasov, Dušan
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/383
AB  - Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia
EP  - 770
IS  - 4
SP  - 759
VL  - 142
DO  - 10.1007/s10658-015-0649-0
ER  - 

@article{
author = "Atanasova, Biljana and Jakovljević, Miljana and Spasov, Dušan and Jović, Jelena and Mitrović, Milana and Toševski, Ivo and Cvrković, Tatjana",
year = "2015",
abstract = "Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia",
pages = "770-759",
number = "4",
volume = "142",
doi = "10.1007/s10658-015-0649-0"
}

Atanasova, B., Jakovljević, M., Spasov, D., Jović, J., Mitrović, M., Toševski, I.,& Cvrković, T.. (2015). The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology
Springer, Dordrecht., 142(4), 759-770.
https://doi.org/10.1007/s10658-015-0649-0

Atanasova B, Jakovljević M, Spasov D, Jović J, Mitrović M, Toševski I, Cvrković T. The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology. 2015;142(4):759-770.
doi:10.1007/s10658-015-0649-0 .

Atanasova, Biljana, Jakovljević, Miljana, Spasov, Dušan, Jović, Jelena, Mitrović, Milana, Toševski, Ivo, Cvrković, Tatjana, "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia" in European Journal of Plant Pathology, 142, no. 4 (2015):759-770,
https://doi.org/10.1007/s10658-015-0649-0 . .

TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/380
AB  - We investigated multiple inflorescence disease of Cirsium arvense (CMI) and its association with phytoplasmas of the 16SrIII-B subgroup, potential natural vector(s) and reservoir plant(s). From five locations in northern Serbia, 27 plants of C. arvense, 1 C. vulgare and 3 Carduus acanthoides with symptoms of multiple inflorescences (MIs) were collected and tested for 16SrIII group phytoplasmas. All symptomatic plants were found to be infected. Tentative reservoir plants and insect vectors were collected at a Dobanovci site where the continuous presence of CMI disease was recorded. Among the 19 most abundant plant species submitted to phytoplasma testing, all symptomless, the presence of the 16SrIII group was detected only in two legumes: Lathyrus tuberosus (2/5) and L. aphaca (1/5). Among 19 insect species from six families of Auchenorrhyncha, the deltocephalid leafhopper Euscelis incisus was the only insect carrying a 16SrIII phytoplasma (10% of analysed individuals). Transmission trials were performed with naturally infected E. incisus adults of the summer generation and with a laboratory population reared on red clover. After an acquisition period of 48 h on C. arvense symptomatic for MIs and a latent period of 28 days, 83% of the E. incisus adults (300/360) were infected with CMI phytoplasma. In two transmission tests, the leafhoppers successfully transmitted the phytoplasma to exposed plants (C. arvense and periwinkle), proving its role as a natural vector. Test plants of C. arvense infected with the 16SrIII-B phytoplasma expressed typical symptoms similar to those observed in the field, such as MIs or the absence of flowering, shortened internodes and plant desiccation. Typical symptoms in infected periwinkles were virescence and phyllody. The molecular characterisation of the CMI phytoplasma isolates from diseased and asymptomatic field-collected plants, vectors, and test plants was performed by sequence analyses of the 16S rRNA, rpl22-rps3 and rpl15-secY genes. Phylogenetic analyses of other members of the 16SrIII group of phytoplasmas indicated closest relatedness with clover yellow edge phytoplasma (CYE) of the 16SrIII-B subgroup.
PB  - Wiley-Blackwell, Hoboken
T2  - Annals of Applied Biology
T1  - Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense
EP  - 419
IS  - 3
SP  - 406
VL  - 167
DO  - 10.1111/aab.12236
ER  - 

@article{
author = "Jakovljević, Miljana and Jović, Jelena and Mitrović, Milana and Krstić, Oliver and Kosovac, Andrea and Toševski, Ivo and Cvrković, Tatjana",
year = "2015",
abstract = "We investigated multiple inflorescence disease of Cirsium arvense (CMI) and its association with phytoplasmas of the 16SrIII-B subgroup, potential natural vector(s) and reservoir plant(s). From five locations in northern Serbia, 27 plants of C. arvense, 1 C. vulgare and 3 Carduus acanthoides with symptoms of multiple inflorescences (MIs) were collected and tested for 16SrIII group phytoplasmas. All symptomatic plants were found to be infected. Tentative reservoir plants and insect vectors were collected at a Dobanovci site where the continuous presence of CMI disease was recorded. Among the 19 most abundant plant species submitted to phytoplasma testing, all symptomless, the presence of the 16SrIII group was detected only in two legumes: Lathyrus tuberosus (2/5) and L. aphaca (1/5). Among 19 insect species from six families of Auchenorrhyncha, the deltocephalid leafhopper Euscelis incisus was the only insect carrying a 16SrIII phytoplasma (10% of analysed individuals). Transmission trials were performed with naturally infected E. incisus adults of the summer generation and with a laboratory population reared on red clover. After an acquisition period of 48 h on C. arvense symptomatic for MIs and a latent period of 28 days, 83% of the E. incisus adults (300/360) were infected with CMI phytoplasma. In two transmission tests, the leafhoppers successfully transmitted the phytoplasma to exposed plants (C. arvense and periwinkle), proving its role as a natural vector. Test plants of C. arvense infected with the 16SrIII-B phytoplasma expressed typical symptoms similar to those observed in the field, such as MIs or the absence of flowering, shortened internodes and plant desiccation. Typical symptoms in infected periwinkles were virescence and phyllody. The molecular characterisation of the CMI phytoplasma isolates from diseased and asymptomatic field-collected plants, vectors, and test plants was performed by sequence analyses of the 16S rRNA, rpl22-rps3 and rpl15-secY genes. Phylogenetic analyses of other members of the 16SrIII group of phytoplasmas indicated closest relatedness with clover yellow edge phytoplasma (CYE) of the 16SrIII-B subgroup.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Annals of Applied Biology",
title = "Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense",
pages = "419-406",
number = "3",
volume = "167",
doi = "10.1111/aab.12236"
}

Jakovljević, M., Jović, J., Mitrović, M., Krstić, O., Kosovac, A., Toševski, I.,& Cvrković, T.. (2015). Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense. in Annals of Applied Biology
Wiley-Blackwell, Hoboken., 167(3), 406-419.
https://doi.org/10.1111/aab.12236

Jakovljević M, Jović J, Mitrović M, Krstić O, Kosovac A, Toševski I, Cvrković T. Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense. in Annals of Applied Biology. 2015;167(3):406-419.
doi:10.1111/aab.12236 .

Jakovljević, Miljana, Jović, Jelena, Mitrović, Milana, Krstić, Oliver, Kosovac, Andrea, Toševski, Ivo, Cvrković, Tatjana, "Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense" in Annals of Applied Biology, 167, no. 3 (2015):406-419,
https://doi.org/10.1111/aab.12236 . .

TY  - JOUR
AU  - Toševski, Ivo
AU  - Caldara, Roberto
AU  - Jović, Jelena
AU  - Hernandez-Vera, Gerardo
AU  - Baviera, Cosimo
AU  - Gassmann, Andre
AU  - Emerson, Brent C.
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/395
AB  - A combined taxonomic, morphological, molecular and biological study revealed that stem-galling weevils from the genus Rhinusa associated with toadflaxes from the genus Linaria (Plantaginaceae) are composed of three different species: Rhinusa pilosa, Rhinusa brondelii and Rhinusa rarasp.n. The authentic field host plants are respectively, Linaria vulgaris, Linaria purpurea and Linaria genistifolia/ Linaria dalmatica. These weevil species can be distinguished from each other by a few subtle morphological characteristics, mainly in the shape of the rostrum and of the integument. An analysis of the mitochondrial [cytochrome oxidase subunit II gene (COII) and 16S ribosomal RNA gene (16S)] and nuclear (elongation factor-1, EF-1) sequence data revealed high genetic divergence among these species. Uncorrected pairwise distances on mtCOII gene were 14.3% between R. pilosa and R. brondelii, 15.7% between R. pilosa and R. rara, while R. brondelii and R. rara were approximately 11% divergent from each other. Divergences obtained on 16S and nuclear EF-1 genes were congruent. However, substantial intraspecific mitochondrial divergence was recorded for all studied populations of R. pilosa s.s. showing two mtDNA lineages, with estimated COII and 16S divergences of 4% and 1.6%, respectively. Nuclear pseudogenes (Numts) and Wolbachia influence, although recorded within both lineages, were excluded as possible causatives of the mtDNA divergence, while EF-1 indicated absence of lineage sorting. Species from the R. pilosa complex are estimated to have diverged from each other approximately 7.2 million years ago (mya; late Miocene), while R. brondelii and R. rara diverged from each other about 4.7 mya (early Pliocene). This published work has been registered in ZooBank, .
PB  - Wiley-Blackwell, Hoboken
T2  - Systematic Entomology
T1  - Host-associated genetic divergence and taxonomy in the Rhinusa pilosa Gyllenhal species complex: an integrative approach
EP  - 287
IS  - 1
SP  - 268
VL  - 40
DO  - 10.1111/syen.12109
ER  - 

@article{
author = "Toševski, Ivo and Caldara, Roberto and Jović, Jelena and Hernandez-Vera, Gerardo and Baviera, Cosimo and Gassmann, Andre and Emerson, Brent C.",
year = "2015",
abstract = "A combined taxonomic, morphological, molecular and biological study revealed that stem-galling weevils from the genus Rhinusa associated with toadflaxes from the genus Linaria (Plantaginaceae) are composed of three different species: Rhinusa pilosa, Rhinusa brondelii and Rhinusa rarasp.n. The authentic field host plants are respectively, Linaria vulgaris, Linaria purpurea and Linaria genistifolia/ Linaria dalmatica. These weevil species can be distinguished from each other by a few subtle morphological characteristics, mainly in the shape of the rostrum and of the integument. An analysis of the mitochondrial [cytochrome oxidase subunit II gene (COII) and 16S ribosomal RNA gene (16S)] and nuclear (elongation factor-1, EF-1) sequence data revealed high genetic divergence among these species. Uncorrected pairwise distances on mtCOII gene were 14.3% between R. pilosa and R. brondelii, 15.7% between R. pilosa and R. rara, while R. brondelii and R. rara were approximately 11% divergent from each other. Divergences obtained on 16S and nuclear EF-1 genes were congruent. However, substantial intraspecific mitochondrial divergence was recorded for all studied populations of R. pilosa s.s. showing two mtDNA lineages, with estimated COII and 16S divergences of 4% and 1.6%, respectively. Nuclear pseudogenes (Numts) and Wolbachia influence, although recorded within both lineages, were excluded as possible causatives of the mtDNA divergence, while EF-1 indicated absence of lineage sorting. Species from the R. pilosa complex are estimated to have diverged from each other approximately 7.2 million years ago (mya; late Miocene), while R. brondelii and R. rara diverged from each other about 4.7 mya (early Pliocene). This published work has been registered in ZooBank, .",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Systematic Entomology",
title = "Host-associated genetic divergence and taxonomy in the Rhinusa pilosa Gyllenhal species complex: an integrative approach",
pages = "287-268",
number = "1",
volume = "40",
doi = "10.1111/syen.12109"
}

Toševski, I., Caldara, R., Jović, J., Hernandez-Vera, G., Baviera, C., Gassmann, A.,& Emerson, B. C.. (2015). Host-associated genetic divergence and taxonomy in the Rhinusa pilosa Gyllenhal species complex: an integrative approach. in Systematic Entomology
Wiley-Blackwell, Hoboken., 40(1), 268-287.
https://doi.org/10.1111/syen.12109

Toševski I, Caldara R, Jović J, Hernandez-Vera G, Baviera C, Gassmann A, Emerson BC. Host-associated genetic divergence and taxonomy in the Rhinusa pilosa Gyllenhal species complex: an integrative approach. in Systematic Entomology. 2015;40(1):268-287.
doi:10.1111/syen.12109 .

Toševski, Ivo, Caldara, Roberto, Jović, Jelena, Hernandez-Vera, Gerardo, Baviera, Cosimo, Gassmann, Andre, Emerson, Brent C., "Host-associated genetic divergence and taxonomy in the Rhinusa pilosa Gyllenhal species complex: an integrative approach" in Systematic Entomology, 40, no. 1 (2015):268-287,
https://doi.org/10.1111/syen.12109 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Stanisavljević, Rade
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Bosković, Jelena
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/416
AB  - The emergence of Cercospora beticola populations that are resistant to benzimidazoles (MBC) and demethylation-inhibiting fungicides (DMI) has been recently reported in Serbia and has resulted in a reduced efficacy of fungicides in controlling Cercospora leaf spot (CLS). Between 2008 and 2011, using a discriminatory concentration method in sugar beet fields in two separate regions of Serbia, we determined that 93.3%-98.6% of collected C. bed cola isolates were resistant to MBCs, whereas 6.2%-42.4% were resistant to DMI fungicides. At the same localities, field trials were conducted to investigate the impact of resistant C. beticola populations on disease management From the MBC group of fungicides, both thiophanate methyl and carbendazim failed to suppress the spread of CLS at both of the tested localities. Between 2008 and 2010, DMI fungicides expressed moderate efficacy at a South Banat locality (79.8%-84.6%) whether they were applied individually (flutriafol, epoxiconazole) or in combination with MBCs (epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole). The frequency of resistant isolates in these test trials ranged from 6.2% to 10.9%. In 2011, at the same locality, conditions were observed to change in favor of the occurrence of resistant populations, which comprised up to 18.7% of the population, at the expense of DMI efficacy in CLS management At a Srem locality, the frequency of C. beticola isolates that were resistant to DMIs was high during all four years of field testing (30.5%-42.4%), and the efficacy of these fungicides ranged from 48.4% to 68.0%. A combination of DMI and a protective chlorothalonil had a stable, moderate impact on disease management regardless of the frequency of DMI resistance, whereas a combination of the cyproconazole DMI with trifloxystrobin from the strobilurin group of fungicides expressed the highest efficacy. High correlation coefficient values (r = 0.87) indicated how strongly the frequencies of resistant populations affected disease severity in the trial plots that were treated with carbendazim and thiophanate methyl, as well as in the plots that were treated with flutriafol and epoxiconazole (r = 0.98). In shift sensitivity trials, MBC-resistant C. beticola isolates were found at equally high frequencies both before and after the treatments, indicating a complete loss of efficacy in CLS control. This test revealed the significant impact of multiple DMI applications in terms of increasing the frequency of resistant C. beticola populations following treatments with flutriafol, epoxiconazole, epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole and flutriafol/chlorothalonil, except when used in combination with trifloxystrobin, in which case CLS suppression was substantially high. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management
EP  - 87
SP  - 80
VL  - 75
DO  - 10.1016/j.cropro.2015.05.017
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Stanisavljević, Rade and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Bosković, Jelena",
year = "2015",
abstract = "The emergence of Cercospora beticola populations that are resistant to benzimidazoles (MBC) and demethylation-inhibiting fungicides (DMI) has been recently reported in Serbia and has resulted in a reduced efficacy of fungicides in controlling Cercospora leaf spot (CLS). Between 2008 and 2011, using a discriminatory concentration method in sugar beet fields in two separate regions of Serbia, we determined that 93.3%-98.6% of collected C. bed cola isolates were resistant to MBCs, whereas 6.2%-42.4% were resistant to DMI fungicides. At the same localities, field trials were conducted to investigate the impact of resistant C. beticola populations on disease management From the MBC group of fungicides, both thiophanate methyl and carbendazim failed to suppress the spread of CLS at both of the tested localities. Between 2008 and 2010, DMI fungicides expressed moderate efficacy at a South Banat locality (79.8%-84.6%) whether they were applied individually (flutriafol, epoxiconazole) or in combination with MBCs (epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole). The frequency of resistant isolates in these test trials ranged from 6.2% to 10.9%. In 2011, at the same locality, conditions were observed to change in favor of the occurrence of resistant populations, which comprised up to 18.7% of the population, at the expense of DMI efficacy in CLS management At a Srem locality, the frequency of C. beticola isolates that were resistant to DMIs was high during all four years of field testing (30.5%-42.4%), and the efficacy of these fungicides ranged from 48.4% to 68.0%. A combination of DMI and a protective chlorothalonil had a stable, moderate impact on disease management regardless of the frequency of DMI resistance, whereas a combination of the cyproconazole DMI with trifloxystrobin from the strobilurin group of fungicides expressed the highest efficacy. High correlation coefficient values (r = 0.87) indicated how strongly the frequencies of resistant populations affected disease severity in the trial plots that were treated with carbendazim and thiophanate methyl, as well as in the plots that were treated with flutriafol and epoxiconazole (r = 0.98). In shift sensitivity trials, MBC-resistant C. beticola isolates were found at equally high frequencies both before and after the treatments, indicating a complete loss of efficacy in CLS control. This test revealed the significant impact of multiple DMI applications in terms of increasing the frequency of resistant C. beticola populations following treatments with flutriafol, epoxiconazole, epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole and flutriafol/chlorothalonil, except when used in combination with trifloxystrobin, in which case CLS suppression was substantially high. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management",
pages = "87-80",
volume = "75",
doi = "10.1016/j.cropro.2015.05.017"
}

Trkulja, N., Milosavljević, A., Stanisavljević, R., Mitrović, M., Jović, J., Toševski, I.,& Bosković, J.. (2015). Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management. in Crop Protection
Elsevier Sci Ltd, Oxford., 75, 80-87.
https://doi.org/10.1016/j.cropro.2015.05.017

Trkulja N, Milosavljević A, Stanisavljević R, Mitrović M, Jović J, Toševski I, Bosković J. Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management. in Crop Protection. 2015;75:80-87.
doi:10.1016/j.cropro.2015.05.017 .

Trkulja, Nenad, Milosavljević, Anja, Stanisavljević, Rade, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Bosković, Jelena, "Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management" in Crop Protection, 75 (2015):80-87,
https://doi.org/10.1016/j.cropro.2015.05.017 . .