Živković, Svetlana

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Authority KeyName Variants
orcid::0000-0003-3651-7485
  • Živković, Svetlana (69)
Projects
Development of integrated approach in plant protection for control harmful organisms Projekat Ministarstva nauke Republike Srbije, br. 20051: Optimizacija primene hemijskih sredstava u zaštiti bilja povećanjem efikasnosti dijagnostičkih metoda i procene rizika pojave bolesti, štetočina i korova
Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control
Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes New indigenous bacterial isolates Lysobacter and Pseudomonas as an important source of metabolites useful for biotechnology, plant growth stimulation and disease control: from isolates to inoculants
Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection
FP7 RegPot project FCUB ERA GA - 256716 The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology
Microbial diversity study and characterization of beneficial environmental microorganisms Development of vegetable cultivars and hybrids intended for outdoor and indoor production

Author's Bibliography

Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia

Stevanović, Miloš; Ristić, Danijela; Živković, Svetlana; Aleksić, Goran; Stanković, Ivana; Krstić, Branka; Bulajić, Aleksandra

(Amer Phytopathological Soc, St Paul, 2019)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Ristić, Danijela
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/570
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 
@article{
author = "Stevanović, Miloš and Ristić, Danijela and Živković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}
Stevanović, M., Ristić, D., Živković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE
Stevanović M, Ristić D, Živković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .
Stevanović, Miloš, Ristić, Danijela, Živković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .
6
2
6

Efficacy of copper citrate in grapevine disease control

Aleksić, Goran; Milićević, Zoran; Kuzmanović, Slobodan; Starović, Mira; Stevanović, Miloš; Delibašić, Goran; Živković, Svetlana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2019)

TY  - JOUR
AU  - Aleksić, Goran
AU  - Milićević, Zoran
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Delibašić, Goran
AU  - Živković, Svetlana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5229
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/599
AB  - The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection.
AB  - Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Efficacy of copper citrate in grapevine disease control
T1  - Efikasnost bakar-citrata u zaštiti vinove loze od bolesti
EP  - 109
IS  - 2
SP  - 103
VL  - 34
ER  - 
@article{
author = "Aleksić, Goran and Milićević, Zoran and Kuzmanović, Slobodan and Starović, Mira and Stevanović, Miloš and Delibašić, Goran and Živković, Svetlana",
year = "2019",
abstract = "The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection., Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Efficacy of copper citrate in grapevine disease control, Efikasnost bakar-citrata u zaštiti vinove loze od bolesti",
pages = "109-103",
number = "2",
volume = "34"
}
Aleksić, G., Milićević, Z., Kuzmanović, S., Starović, M., Stevanović, M., Delibašić, G.,& Živković, S.. (2019). Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(2), 103-109.
Aleksić G, Milićević Z, Kuzmanović S, Starović M, Stevanović M, Delibašić G, Živković S. Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina. 2019;34(2):103-109..
Aleksić, Goran, Milićević, Zoran, Kuzmanović, Slobodan, Starović, Mira, Stevanović, Miloš, Delibašić, Goran, Živković, Svetlana, "Efficacy of copper citrate in grapevine disease control" in Pesticidi i fitomedicina, 34, no. 2 (2019):103-109.

Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides

Živković, Svetlana; Stevanović, Miloš; Đurović, Sanja; Ristić, Danijela; Stošić, Stefan

(Institute of Pesticides and Environmental Protection, 2018)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 
@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}
Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z
Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .
Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .
4

Effects of copper citrate as a defoliant in nursery fruit stock production

Gavrilović, Veljko; Milićević, Zoran; Aleksić, Goran; Starović, Mira; Živković, Svetlana; Stošić, Stefan; Kuzmanović, Slobodan

(Institute of Pesticides and Environmental Protection, 2017)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/495
AB  - The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks.
AB  - U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Effects of copper citrate as a defoliant in nursery fruit stock production
T1  - Bakar citrat - defolijant u proizvodnji sadnica voćaka
EP  - 236
IS  - 3-4
SP  - 231
VL  - 32
DO  - 10.2298/PIF1704231G
ER  - 
@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksić, Goran and Starović, Mira and Živković, Svetlana and Stošić, Stefan and Kuzmanović, Slobodan",
year = "2017",
abstract = "The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks., U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Effects of copper citrate as a defoliant in nursery fruit stock production, Bakar citrat - defolijant u proizvodnji sadnica voćaka",
pages = "236-231",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704231G"
}
Gavrilović, V., Milićević, Z., Aleksić, G., Starović, M., Živković, S., Stošić, S.,& Kuzmanović, S.. (2017). Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 231-236.
https://doi.org/10.2298/PIF1704231G
Gavrilović V, Milićević Z, Aleksić G, Starović M, Živković S, Stošić S, Kuzmanović S. Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina. 2017;32(3-4):231-236.
doi:10.2298/PIF1704231G .
Gavrilović, Veljko, Milićević, Zoran, Aleksić, Goran, Starović, Mira, Živković, Svetlana, Stošić, Stefan, Kuzmanović, Slobodan, "Effects of copper citrate as a defoliant in nursery fruit stock production" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):231-236,
https://doi.org/10.2298/PIF1704231G . .
3
1

Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin

Milisavljević, M.; Živković, Svetlana; Pekmezović, M.; Stanković, N.; Vojnović, S.; Vasiljević, B.; Senerović, L.

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Milisavljević, M.
AU  - Živković, Svetlana
AU  - Pekmezović, M.
AU  - Stanković, N.
AU  - Vojnović, S.
AU  - Vasiljević, B.
AU  - Senerović, L.
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/378
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 
@article{
author = "Milisavljević, M. and Živković, Svetlana and Pekmezović, M. and Stanković, N. and Vojnović, S. and Vasiljević, B. and Senerović, L.",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}
Milisavljević, M., Živković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Senerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811
Milisavljević M, Živković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Senerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .
Milisavljević, M., Živković, Svetlana, Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B., Senerović, L., "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .
3
2
3

First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.

Trkulja, Nenad; Milosavljević, Anja; Živković, Svetlana; Popović, Tatjana; Mitrović, Milana; Jović, Jelena; Toševski, Ivo

(Amer Phytopathological Soc, St Paul, 2015)

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Živković, Svetlana
AU  - Popović, Tatjana
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/397
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.
EP  - 1036
IS  - 7
SP  - 1035
VL  - 99
DO  - 10.1094/PDIS-10-14-1096-PDN
ER  - 
@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Živković, Svetlana and Popović, Tatjana and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.",
pages = "1036-1035",
number = "7",
volume = "99",
doi = "10.1094/PDIS-10-14-1096-PDN"
}
Trkulja, N., Milosavljević, A., Živković, S., Popović, T., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(7), 1035-1036.
https://doi.org/10.1094/PDIS-10-14-1096-PDN
Trkulja N, Milosavljević A, Živković S, Popović T, Mitrović M, Jović J, Toševski I. First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease. 2015;99(7):1035-1036.
doi:10.1094/PDIS-10-14-1096-PDN .
Trkulja, Nenad, Milosavljević, Anja, Živković, Svetlana, Popović, Tatjana, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia." in Plant Disease, 99, no. 7 (2015):1035-1036,
https://doi.org/10.1094/PDIS-10-14-1096-PDN . .

Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia

Gašić, Katarina; Gavrilović, Veljko; Dolovac, Nenad; Trkulja, Nenad; Živković, Svetlana; Ristić, Danijela; Obradović, Aleksa

(Institute of Pesticides and Environmental Protection, 2014)

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/362
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 
@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}
Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G
Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .
Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .
9

Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose

Živković, Svetlana; Stojanović, Saša; Balaž, Jelica

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stojanović, Saša
AU  - Balaž, Jelica
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/305
AB  - In the morphological studies were included isolates of Colletotrichum spp. originating from pear, apple and sour cherry fruits, as well as reference strains of C. acutatum (CBS 294.67) and C. gloeosporioides (CBS 516.97). The colony characteristics, appearance, shape and size of conidioma, appressoria, and conidia of isolates of Colletotrichum spp. were studied. The four morphological groups of colonies on PDA were observed, confirming the great phenotypic variability of isolates of Colletotrichum spp. Most isolates from apple and pear fruits (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9), formed the first morphological group. The isolates from sour cherry (VC-3, VC-5, VC-7, VC-9) are separated in the fourth morphological group. The size and shape of conidia from these isolates fit the description of C. gloe­osporioides. The chromogenic isolates from pear (KC-21, KC-23, KC-82) formed the second morphological group, and in the third morphological group is isolate (JC-4) from apple fruit. The morphology of conidia from these isolates fit the description of C. acutatum. During this research were evident that all isolates formed conidioma - acervuli. The tested cultures of Colletotrichum spp. did not form teleomorpf. Because of outstanding variability, most of the studied characteristics cannot be independently applied as taxonomic criteria. Obtai­ned results showed that, for Colletotrichum species classification, at least two taxonomic criteria should be used: morphological features combined with molecular identification.
AB  - U morfološka ispitivanja su uključeni izolati Colletotrichum spp. poreklom sa plodova kruške, jabuke i višnje, kao i referentni sojevi C. acutatum (CBS 294.67) i C. gloeosporioides (CBS 516.97). Proučavanjem njihovih morfoloških karakteristika, dobijeni su podaci o izgledu ispitivanih kolonija, odlikama plodonosnog tela, apresorija, veličini i obliku konidija. Na podlozi KDA moguće je razlikovati četiri morfološke grupe kolonija, što potvrđu­je fenotipsku varijabilnost proučavanih izolata Colletotrichum spp. Većina izolata sa ploda kruške i jabuke (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9) obrazuje prvu morfološku grupu. Izolati sa ploda višnje (VC-3, VC-5, VC-7, VC-9), su izdvojeni u četvrtu morfološku grupu. Veličina i oblik konidija ovih izolata odgovara opisu vrste C. gloeosporioides. Hromogeni izolati sa kruške (KC-21, KC-23, KC-82), obrazuju drugu morfološku grupu, a u trećoj morfološkoj grupi je izolat sa ploda jabuke (JC-4). Morfologija konidija ovih izolata odgovara opisu vrste C. acutatum. Tokom ovih istraživanja je utvrđeno da svi izolati obrazuju plodonosna tela - acervule. Testirane kulture Colletotrichum spp. ne formiraju teleomorfni stadijum. Zbog izražene varijabilnosti, većina ispitanih osobina ne mogu se primeni kao samostalni taksonomski kriterijum. Na osnovu dobijenih rezultata, za klasifikaciju vrsta roda Colletotrichum, potrebno je koristiti najmanje dva taksonomska kriterijuma: morfološke karakteristike uporedo sa molekularnom identifikacijom.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose
T1  - Morfološke karakteristike izolata Colletotrichum spp. - prouzrokovača antraknoze
EP  - 136
IS  - 3
SP  - 124
VL  - 65
DO  - 10.5937/zasbilj1403124Z
ER  - 
@article{
author = "Živković, Svetlana and Stojanović, Saša and Balaž, Jelica",
year = "2014",
abstract = "In the morphological studies were included isolates of Colletotrichum spp. originating from pear, apple and sour cherry fruits, as well as reference strains of C. acutatum (CBS 294.67) and C. gloeosporioides (CBS 516.97). The colony characteristics, appearance, shape and size of conidioma, appressoria, and conidia of isolates of Colletotrichum spp. were studied. The four morphological groups of colonies on PDA were observed, confirming the great phenotypic variability of isolates of Colletotrichum spp. Most isolates from apple and pear fruits (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9), formed the first morphological group. The isolates from sour cherry (VC-3, VC-5, VC-7, VC-9) are separated in the fourth morphological group. The size and shape of conidia from these isolates fit the description of C. gloe­osporioides. The chromogenic isolates from pear (KC-21, KC-23, KC-82) formed the second morphological group, and in the third morphological group is isolate (JC-4) from apple fruit. The morphology of conidia from these isolates fit the description of C. acutatum. During this research were evident that all isolates formed conidioma - acervuli. The tested cultures of Colletotrichum spp. did not form teleomorpf. Because of outstanding variability, most of the studied characteristics cannot be independently applied as taxonomic criteria. Obtai­ned results showed that, for Colletotrichum species classification, at least two taxonomic criteria should be used: morphological features combined with molecular identification., U morfološka ispitivanja su uključeni izolati Colletotrichum spp. poreklom sa plodova kruške, jabuke i višnje, kao i referentni sojevi C. acutatum (CBS 294.67) i C. gloeosporioides (CBS 516.97). Proučavanjem njihovih morfoloških karakteristika, dobijeni su podaci o izgledu ispitivanih kolonija, odlikama plodonosnog tela, apresorija, veličini i obliku konidija. Na podlozi KDA moguće je razlikovati četiri morfološke grupe kolonija, što potvrđu­je fenotipsku varijabilnost proučavanih izolata Colletotrichum spp. Većina izolata sa ploda kruške i jabuke (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9) obrazuje prvu morfološku grupu. Izolati sa ploda višnje (VC-3, VC-5, VC-7, VC-9), su izdvojeni u četvrtu morfološku grupu. Veličina i oblik konidija ovih izolata odgovara opisu vrste C. gloeosporioides. Hromogeni izolati sa kruške (KC-21, KC-23, KC-82), obrazuju drugu morfološku grupu, a u trećoj morfološkoj grupi je izolat sa ploda jabuke (JC-4). Morfologija konidija ovih izolata odgovara opisu vrste C. acutatum. Tokom ovih istraživanja je utvrđeno da svi izolati obrazuju plodonosna tela - acervule. Testirane kulture Colletotrichum spp. ne formiraju teleomorfni stadijum. Zbog izražene varijabilnosti, većina ispitanih osobina ne mogu se primeni kao samostalni taksonomski kriterijum. Na osnovu dobijenih rezultata, za klasifikaciju vrsta roda Colletotrichum, potrebno je koristiti najmanje dva taksonomska kriterijuma: morfološke karakteristike uporedo sa molekularnom identifikacijom.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose, Morfološke karakteristike izolata Colletotrichum spp. - prouzrokovača antraknoze",
pages = "136-124",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403124Z"
}
Živković, S., Stojanović, S.,& Balaž, J.. (2014). Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 124-136.
https://doi.org/10.5937/zasbilj1403124Z
Živković S, Stojanović S, Balaž J. Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose. in Zaštita bilja. 2014;65(3):124-136.
doi:10.5937/zasbilj1403124Z .
Živković, Svetlana, Stojanović, Saša, Balaž, Jelica, "Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose" in Zaštita bilja, 65, no. 3 (2014):124-136,
https://doi.org/10.5937/zasbilj1403124Z . .

Effect of calcium salts on postharvest fungal pathogens in vitro

Stošić, Stefan; Stojanović, Saša; Milosavljević, Anja; Pfaf-Dolovac, Erika; Živković, Svetlana

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Stošić, Stefan
AU  - Stojanović, Saša
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/329
AB  - In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum.
AB  - Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Effect of calcium salts on postharvest fungal pathogens in vitro
T1  - In vitro efekat kalcijumovih soli na gljivične skladišne patogene
EP  - 46
IS  - 1
SP  - 40
VL  - 65
DO  - 10.5937/zasbilj1401040S
ER  - 
@article{
author = "Stošić, Stefan and Stojanović, Saša and Milosavljević, Anja and Pfaf-Dolovac, Erika and Živković, Svetlana",
year = "2014",
abstract = "In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum., Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Effect of calcium salts on postharvest fungal pathogens in vitro, In vitro efekat kalcijumovih soli na gljivične skladišne patogene",
pages = "46-40",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401040S"
}
Stošić, S., Stojanović, S., Milosavljević, A., Pfaf-Dolovac, E.,& Živković, S.. (2014). Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 40-46.
https://doi.org/10.5937/zasbilj1401040S
Stošić S, Stojanović S, Milosavljević A, Pfaf-Dolovac E, Živković S. Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja. 2014;65(1):40-46.
doi:10.5937/zasbilj1401040S .
Stošić, Stefan, Stojanović, Saša, Milosavljević, Anja, Pfaf-Dolovac, Erika, Živković, Svetlana, "Effect of calcium salts on postharvest fungal pathogens in vitro" in Zaštita bilja, 65, no. 1 (2014):40-46,
https://doi.org/10.5937/zasbilj1401040S . .
4

First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia

Živković, Svetlana; Gavrilović, Veljko; Popović, Tatjana; Dolovac, Nenad; Trkulja, Nenad

(Amer Phytopathological Soc, St Paul, 2014)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Popović, Tatjana
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/337
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia
EP  - 1272
IS  - 9
SP  - 1272
VL  - 98
DO  - 10.1094/PDIS-01-14-0052-PDN
ER  - 
@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Popović, Tatjana and Dolovac, Nenad and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia",
pages = "1272-1272",
number = "9",
volume = "98",
doi = "10.1094/PDIS-01-14-0052-PDN"
}
Živković, S., Gavrilović, V., Popović, T., Dolovac, N.,& Trkulja, N.. (2014). First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(9), 1272-1272.
https://doi.org/10.1094/PDIS-01-14-0052-PDN
Živković S, Gavrilović V, Popović T, Dolovac N, Trkulja N. First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease. 2014;98(9):1272-1272.
doi:10.1094/PDIS-01-14-0052-PDN .
Živković, Svetlana, Gavrilović, Veljko, Popović, Tatjana, Dolovac, Nenad, Trkulja, Nenad, "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia" in Plant Disease, 98, no. 9 (2014):1272-1272,
https://doi.org/10.1094/PDIS-01-14-0052-PDN . .
3
1
2

Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method

Gavrilović, Veljko; Ivanović, Žarko; Popović, Tatjana; Živković, Svetlana

(Int Soc Horticultural Science, Leuven 1, 2014)

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/320
AB  - Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - XIII International Workshop on Fire Blight
T1  - Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method
EP  - 172
SP  - 169
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.26
ER  - 
@conference{
author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana",
year = "2014",
abstract = "Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XIII International Workshop on Fire Blight",
title = "Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method",
pages = "172-169",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.26"
}
Gavrilović, V., Ivanović, Ž., Popović, T.,& Živković, S.. (2014). Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method. in XIII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 1056, 169-172.
https://doi.org/10.17660/ActaHortic.2014.1056.26
Gavrilović V, Ivanović Ž, Popović T, Živković S. Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method. in XIII International Workshop on Fire Blight. 2014;1056:169-172.
doi:10.17660/ActaHortic.2014.1056.26 .
Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, "Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method" in XIII International Workshop on Fire Blight, 1056 (2014):169-172,
https://doi.org/10.17660/ActaHortic.2014.1056.26 . .
1
1

Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria

Nikolić, Ivan; Ivanović, Žarko; Blagojević, Jovana; Živković, Svetlana; Popović, Tatjana

(Institute for Plant Protection and Environment, Belgrade, 2013)

TY  - JOUR
AU  - Nikolić, Ivan
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Živković, Svetlana
AU  - Popović, Tatjana
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/259
AB  - Biological control is an innovative, cost effective and eco-friendly approach for control of many plant diseases. Bacillus sp. and Trichoderma sp. were known for its mycoparasitic and antagonistic mechanism in the control of wide range of phytopathogenic diseases in many types of crops. This research is based on the antibacterial activities of some Bacillus spp. and Trichoderma harzianum in relation to the 10 selected phytopathogenic bacteria in vitro study. The results indicated that among 52 strains of Bacillus spp. presented against phytopathogenic bacteria, only 6 of them showed inhibition but only against Xanthomonas genera. Other Bacillus spp. strains weren't effective against tested phytopathogenic bacteria. Results of testing of T. harzianum efficacy against the growth of selected phytopathogenic bacteria showed that this fungi could be effective to strains from genera Pseudomonas and Xanthomonas but for other tested genera was ineffective. In further work, all of this trial need to be supported by evaluation of antimicrobial activity in vivo.
AB  - Biološka kontrola predstavlja inovativni, isplativ, ekološki pristup za suzbijanje mnogih biljnih bolesti. Vrste roda Bacillus i Trichoderma su poznati po svom mikoparazitskom i antagonističkom mehanizmu suzbijanja širokog spektra fitopatogenih prouzrokovača bolesti kod brojnih poljoprivrednih kultura. U ovom radu proučavano je baktericidno delovanje nekih vrsta roda Bacillus, kao i gljive Trichoderma harzianum na 10 odabranih fitopatogenih bakterija u in vitro uslovima. Prema dobijenim rezultatima, od ukupno 52 testirana izolata iz roda Bacillus, samo 6 je inhibiralo porast fitopatogenih bakterija i to samo iz roda Xanthomonas. Rezultati ispitivanja efikasnosti gljive T. harzianum su ukazali na mogućnost delovanja ove gljive na bakterije iz roda Pseudomonas i Xanthomonas, ali ne i na druge rodove fitopatogenih bakterija testirane u ovom radu. Dalja istraživanja treba bazirati na proučavanjima antimikrobne aktivnosti testiranih agenasa prema fitopatogenim bakterijama u in vivo ogledima.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria
T1  - Baktericidno delovanje nekih Bacillus spp. i Trichoderma harzianum na fitopatogene bakterije
EP  - 197
IS  - 4
SP  - 189
VL  - 64
ER  - 
@article{
author = "Nikolić, Ivan and Ivanović, Žarko and Blagojević, Jovana and Živković, Svetlana and Popović, Tatjana",
year = "2013",
abstract = "Biological control is an innovative, cost effective and eco-friendly approach for control of many plant diseases. Bacillus sp. and Trichoderma sp. were known for its mycoparasitic and antagonistic mechanism in the control of wide range of phytopathogenic diseases in many types of crops. This research is based on the antibacterial activities of some Bacillus spp. and Trichoderma harzianum in relation to the 10 selected phytopathogenic bacteria in vitro study. The results indicated that among 52 strains of Bacillus spp. presented against phytopathogenic bacteria, only 6 of them showed inhibition but only against Xanthomonas genera. Other Bacillus spp. strains weren't effective against tested phytopathogenic bacteria. Results of testing of T. harzianum efficacy against the growth of selected phytopathogenic bacteria showed that this fungi could be effective to strains from genera Pseudomonas and Xanthomonas but for other tested genera was ineffective. In further work, all of this trial need to be supported by evaluation of antimicrobial activity in vivo., Biološka kontrola predstavlja inovativni, isplativ, ekološki pristup za suzbijanje mnogih biljnih bolesti. Vrste roda Bacillus i Trichoderma su poznati po svom mikoparazitskom i antagonističkom mehanizmu suzbijanja širokog spektra fitopatogenih prouzrokovača bolesti kod brojnih poljoprivrednih kultura. U ovom radu proučavano je baktericidno delovanje nekih vrsta roda Bacillus, kao i gljive Trichoderma harzianum na 10 odabranih fitopatogenih bakterija u in vitro uslovima. Prema dobijenim rezultatima, od ukupno 52 testirana izolata iz roda Bacillus, samo 6 je inhibiralo porast fitopatogenih bakterija i to samo iz roda Xanthomonas. Rezultati ispitivanja efikasnosti gljive T. harzianum su ukazali na mogućnost delovanja ove gljive na bakterije iz roda Pseudomonas i Xanthomonas, ali ne i na druge rodove fitopatogenih bakterija testirane u ovom radu. Dalja istraživanja treba bazirati na proučavanjima antimikrobne aktivnosti testiranih agenasa prema fitopatogenim bakterijama u in vivo ogledima.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria, Baktericidno delovanje nekih Bacillus spp. i Trichoderma harzianum na fitopatogene bakterije",
pages = "197-189",
number = "4",
volume = "64"
}
Nikolić, I., Ivanović, Ž., Blagojević, J., Živković, S.,& Popović, T.. (2013). Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(4), 189-197.
Nikolić I, Ivanović Ž, Blagojević J, Živković S, Popović T. Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria. in Zaštita bilja. 2013;64(4):189-197..
Nikolić, Ivan, Ivanović, Žarko, Blagojević, Jovana, Živković, Svetlana, Popović, Tatjana, "Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria" in Zaštita bilja, 64, no. 4 (2013):189-197.

Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria

Delić, Dušica; Stajković-Srbinović, Olivera; Živković, Svetlana; Protić, Nada; Rasulić, Nataša; Kuzmanović, Đorđe; Simić, Aleksandar

(Institute for Plant Protection and Environment, Belgrade, 2013)

TY  - JOUR
AU  - Delić, Dušica
AU  - Stajković-Srbinović, Olivera
AU  - Živković, Svetlana
AU  - Protić, Nada
AU  - Rasulić, Nataša
AU  - Kuzmanović, Đorđe
AU  - Simić, Aleksandar
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/267
AB  - In the greenhouse experiment, the possibility of alfalfa (Medicago sativa L.) growth promotion by inoculation of preceding barley (Hordeum vulgare L.), with plant growth promoting rhizobacteria (PGPR) was examined. The aim of experiment was to select the effective strains as biofertilizer applied in plant rotation. Effects of inoculation with two Azotobacter and two Pseudomonas strains as well as one Sinorhizobium, Enterobacter and Bacillus strain on shoot dry weight and total N content of alfalfa were determined. The results pointed out significant plant growth promotion abilities of strains A1, A2 and P1 which increased alfalfa shoot dry weight over untreated control Ø, by 41, 39 and 35 %, respectively. These three strains increased total N content of alfalfa plants by 34.92 - 40.45% in respect to control Ø. The presented study showed a significant positive influence of preceding barley inoculation with rhizobacteria alone and their mixture on shoot yield and total N content of alfalfa. Results indicated that strains of Azotobacter sp., Pseudomonas sp. and Enterobacter sp. alone can be investigated in further researches as potential agents of biofertilizer for plant growth promotion of alfalfa.
AB  - U eksprimentu u sudovima ispitana je mogućnost poboljšanja rasta lucerke (Medicago sativa L.) pomoću inokulacije ječma (Hordeum vulgare L.) kao preduseva bakterijama koje poboljšavaju rast biljaka (PGPR). Cilj eksperimenta je bio odabiranje efikasnih sojeva koji bi se primenili u plodoredu u formi biološkog đubriva. U radu je korišćeno sedam sojeva koji pripadaju sledećim bakterijskim vrstama: Sinorhizobium meliloti, Bacillus megaterium, Enterobacter sp, Azotobacter sp, kao i vrsti Pseudomonas sp. Efekat inokulacije ječma primenjenim sojevima je određen na osnovu suve nadzemne mase (SDW) i sadržaja ukupnog azota (N) u biljnoj masi lucerke. Rezultati su ukazali na sposobnost nekih sojeva da poboljšaju rast biljaka. Značajno je povećan prinos lucerke u odnosu na kontrolu Ø (lucerka gajena posle neinokulisanog ječma kao preduseva) inokulacijom sojevima Azotobacter-a A1 (41%) i A2 (39%) i Pseudomonas-a P1 (35%). Sadržaj ukupnog N je bio u korelaciji sa vrednostima SDW. Rezultati su ukazali na uticaj inokulacije ječma kao preduseva na prinos lucerke i njen kvalitet kao i da sojevi Azotobacter sp., Pseudomonas sp. i Enterobacter sp. imaju PGPR potencijal što daje osnovu za dalja ispitivanja i mogućnost primene kao bio­đubriva.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria
T1  - Poboljšanje rasta lucerke, Medicago sativa L. - pomoću inokulacije preduseva rizosfernim bakterijama
EP  - 42
IS  - 1
SP  - 35
VL  - 64
ER  - 
@article{
author = "Delić, Dušica and Stajković-Srbinović, Olivera and Živković, Svetlana and Protić, Nada and Rasulić, Nataša and Kuzmanović, Đorđe and Simić, Aleksandar",
year = "2013",
abstract = "In the greenhouse experiment, the possibility of alfalfa (Medicago sativa L.) growth promotion by inoculation of preceding barley (Hordeum vulgare L.), with plant growth promoting rhizobacteria (PGPR) was examined. The aim of experiment was to select the effective strains as biofertilizer applied in plant rotation. Effects of inoculation with two Azotobacter and two Pseudomonas strains as well as one Sinorhizobium, Enterobacter and Bacillus strain on shoot dry weight and total N content of alfalfa were determined. The results pointed out significant plant growth promotion abilities of strains A1, A2 and P1 which increased alfalfa shoot dry weight over untreated control Ø, by 41, 39 and 35 %, respectively. These three strains increased total N content of alfalfa plants by 34.92 - 40.45% in respect to control Ø. The presented study showed a significant positive influence of preceding barley inoculation with rhizobacteria alone and their mixture on shoot yield and total N content of alfalfa. Results indicated that strains of Azotobacter sp., Pseudomonas sp. and Enterobacter sp. alone can be investigated in further researches as potential agents of biofertilizer for plant growth promotion of alfalfa., U eksprimentu u sudovima ispitana je mogućnost poboljšanja rasta lucerke (Medicago sativa L.) pomoću inokulacije ječma (Hordeum vulgare L.) kao preduseva bakterijama koje poboljšavaju rast biljaka (PGPR). Cilj eksperimenta je bio odabiranje efikasnih sojeva koji bi se primenili u plodoredu u formi biološkog đubriva. U radu je korišćeno sedam sojeva koji pripadaju sledećim bakterijskim vrstama: Sinorhizobium meliloti, Bacillus megaterium, Enterobacter sp, Azotobacter sp, kao i vrsti Pseudomonas sp. Efekat inokulacije ječma primenjenim sojevima je određen na osnovu suve nadzemne mase (SDW) i sadržaja ukupnog azota (N) u biljnoj masi lucerke. Rezultati su ukazali na sposobnost nekih sojeva da poboljšaju rast biljaka. Značajno je povećan prinos lucerke u odnosu na kontrolu Ø (lucerka gajena posle neinokulisanog ječma kao preduseva) inokulacijom sojevima Azotobacter-a A1 (41%) i A2 (39%) i Pseudomonas-a P1 (35%). Sadržaj ukupnog N je bio u korelaciji sa vrednostima SDW. Rezultati su ukazali na uticaj inokulacije ječma kao preduseva na prinos lucerke i njen kvalitet kao i da sojevi Azotobacter sp., Pseudomonas sp. i Enterobacter sp. imaju PGPR potencijal što daje osnovu za dalja ispitivanja i mogućnost primene kao bio­đubriva.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria, Poboljšanje rasta lucerke, Medicago sativa L. - pomoću inokulacije preduseva rizosfernim bakterijama",
pages = "42-35",
number = "1",
volume = "64"
}
Delić, D., Stajković-Srbinović, O., Živković, S., Protić, N., Rasulić, N., Kuzmanović, Đ.,& Simić, A.. (2013). Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(1), 35-42.
Delić D, Stajković-Srbinović O, Živković S, Protić N, Rasulić N, Kuzmanović Đ, Simić A. Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria. in Zaštita bilja. 2013;64(1):35-42..
Delić, Dušica, Stajković-Srbinović, Olivera, Živković, Svetlana, Protić, Nada, Rasulić, Nataša, Kuzmanović, Đorđe, Simić, Aleksandar, "Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria" in Zaštita bilja, 64, no. 1 (2013):35-42.

Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia

Gavrilović, Veljko; Ivanović, Žarko; Popović, Tatjana; Živković, Svetlana; Stanković, Slaviša; Berić, Tanja; Fira, Đorđe

(Serbian Genetics Society, 2013)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Živković, Svetlana
AU  - Stanković, Slaviša
AU  - Berić, Tanja
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/251
AB  - During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia.
AB  - Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia
T1  - Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji
EP  - 961
IS  - 3
SP  - 953
VL  - 45
DO  - 10.2298/GENSR1303953G
ER  - 
@article{
author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana and Stanković, Slaviša and Berić, Tanja and Fira, Đorđe",
year = "2013",
abstract = "During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia., Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia, Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji",
pages = "961-953",
number = "3",
volume = "45",
doi = "10.2298/GENSR1303953G"
}
Gavrilović, V., Ivanović, Ž., Popović, T., Živković, S., Stanković, S., Berić, T.,& Fira, Đ.. (2013). Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 45(3), 953-961.
https://doi.org/10.2298/GENSR1303953G
Gavrilović V, Ivanović Ž, Popović T, Živković S, Stanković S, Berić T, Fira Đ. Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade. 2013;45(3):953-961.
doi:10.2298/GENSR1303953G .
Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia" in Genetika-Belgrade, 45, no. 3 (2013):953-961,
https://doi.org/10.2298/GENSR1303953G . .
1
2
3

Biological effectiveness of plasma and electromagnetic field seeds treatments against plant pathogenic bacteria and fungi

Filatova, I.I.; Azharonok, V.V.; Goncharik, S.V.; Lyushkevich, V.A.; Zhukovsky, A.G.; Gadzhieva, G.I.; Spasić, K.; Živković, Svetlana; Puač, N.; Lazović, S.; Malović, G.; Petrović, Z.Lj.

(Brno : Brno University of Technology, 2013)

TY  - CONF
AU  - Filatova, I.I.
AU  - Azharonok, V.V.
AU  - Goncharik, S.V.
AU  - Lyushkevich, V.A.
AU  - Zhukovsky, A.G.
AU  - Gadzhieva, G.I.
AU  - Spasić, K.
AU  - Živković, Svetlana
AU  - Puač, N.
AU  - Lazović, S.
AU  - Malović, G.
AU  - Petrović, Z.Lj.
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/290
AB  - An influence of RF air plasma and RF electromagnetic field pre-sowing treatments on germination and level of fungal and bacterial infection of seeds of some important agricultural plants has been studied. Two plasma systems (plan-parallel and cylindrical) were used for treatment. It is shown that the treatments provide a fungicidal and bactericidal effects and positively influenced seeds germination. The modification of seed coat surface structure is investigated with scanning electron microscopy.
PB  - Brno : Brno University of Technology
C3  - 20th Symposium on Physics of Switching Arc 2013, FSO 2013
T1  - Biological effectiveness of plasma and electromagnetic field seeds treatments against plant pathogenic bacteria and fungi
EP  - 138
SP  - 135
ER  - 
@conference{
author = "Filatova, I.I. and Azharonok, V.V. and Goncharik, S.V. and Lyushkevich, V.A. and Zhukovsky, A.G. and Gadzhieva, G.I. and Spasić, K. and Živković, Svetlana and Puač, N. and Lazović, S. and Malović, G. and Petrović, Z.Lj.",
year = "2013",
abstract = "An influence of RF air plasma and RF electromagnetic field pre-sowing treatments on germination and level of fungal and bacterial infection of seeds of some important agricultural plants has been studied. Two plasma systems (plan-parallel and cylindrical) were used for treatment. It is shown that the treatments provide a fungicidal and bactericidal effects and positively influenced seeds germination. The modification of seed coat surface structure is investigated with scanning electron microscopy.",
publisher = "Brno : Brno University of Technology",
journal = "20th Symposium on Physics of Switching Arc 2013, FSO 2013",
title = "Biological effectiveness of plasma and electromagnetic field seeds treatments against plant pathogenic bacteria and fungi",
pages = "138-135"
}
Filatova, I.I., Azharonok, V.V., Goncharik, S.V., Lyushkevich, V.A., Zhukovsky, A.G., Gadzhieva, G.I., Spasić, K., Živković, S., Puač, N., Lazović, S., Malović, G.,& Petrović, Z.Lj.. (2013). Biological effectiveness of plasma and electromagnetic field seeds treatments against plant pathogenic bacteria and fungi. in 20th Symposium on Physics of Switching Arc 2013, FSO 2013
Brno : Brno University of Technology., 135-138.
Filatova I, Azharonok V, Goncharik S, Lyushkevich V, Zhukovsky A, Gadzhieva G, Spasić K, Živković S, Puač N, Lazović S, Malović G, Petrović Z. Biological effectiveness of plasma and electromagnetic field seeds treatments against plant pathogenic bacteria and fungi. in 20th Symposium on Physics of Switching Arc 2013, FSO 2013. 2013;:135-138..
Filatova, I.I., Azharonok, V.V., Goncharik, S.V., Lyushkevich, V.A., Zhukovsky, A.G., Gadzhieva, G.I., Spasić, K., Živković, Svetlana, Puač, N., Lazović, S., Malović, G., Petrović, Z.Lj., "Biological effectiveness of plasma and electromagnetic field seeds treatments against plant pathogenic bacteria and fungi" in 20th Symposium on Physics of Switching Arc 2013, FSO 2013 (2013):135-138.

Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi

Dimkić, Ivica; Živković, Svetlana; Berić, Tanja; Ivanović, Žarko; Gavrilović, Veljko; Stanković, Slaviša; Fira, Đorđe

(Academic Press Inc Elsevier Science, San Diego, 2013)

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Živković, Svetlana
AU  - Berić, Tanja
AU  - Ivanović, Žarko
AU  - Gavrilović, Veljko
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/291
AB  - Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi
EP  - 321
IS  - 3
SP  - 312
VL  - 65
DO  - 10.1016/j.biocontrol.2013.03.012
ER  - 
@article{
author = "Dimkić, Ivica and Živković, Svetlana and Berić, Tanja and Ivanović, Žarko and Gavrilović, Veljko and Stanković, Slaviša and Fira, Đorđe",
year = "2013",
abstract = "Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi",
pages = "321-312",
number = "3",
volume = "65",
doi = "10.1016/j.biocontrol.2013.03.012"
}
Dimkić, I., Živković, S., Berić, T., Ivanović, Ž., Gavrilović, V., Stanković, S.,& Fira, Đ.. (2013). Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 65(3), 312-321.
https://doi.org/10.1016/j.biocontrol.2013.03.012
Dimkić I, Živković S, Berić T, Ivanović Ž, Gavrilović V, Stanković S, Fira Đ. Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control. 2013;65(3):312-321.
doi:10.1016/j.biocontrol.2013.03.012 .
Dimkić, Ivica, Živković, Svetlana, Berić, Tanja, Ivanović, Žarko, Gavrilović, Veljko, Stanković, Slaviša, Fira, Đorđe, "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi" in Biological Control, 65, no. 3 (2013):312-321,
https://doi.org/10.1016/j.biocontrol.2013.03.012 . .
84
61
85

First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.

Popović, Tatjana; Ivanović, Žarko; Živković, Svetlana; Trkulja, Nenad; Ignjatov, Maja

(Amer Phytopathological Soc, St Paul, 2013)

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Ignjatov, Maja
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/283
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.
EP  - 1504
IS  - 11
SP  - 1504
VL  - 97
DO  - 10.1094/PDIS-03-13-0267-PDN
ER  - 
@article{
author = "Popović, Tatjana and Ivanović, Žarko and Živković, Svetlana and Trkulja, Nenad and Ignjatov, Maja",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.",
pages = "1504-1504",
number = "11",
volume = "97",
doi = "10.1094/PDIS-03-13-0267-PDN"
}
Popović, T., Ivanović, Ž., Živković, S., Trkulja, N.,& Ignjatov, M.. (2013). First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(11), 1504-1504.
https://doi.org/10.1094/PDIS-03-13-0267-PDN
Popović T, Ivanović Ž, Živković S, Trkulja N, Ignjatov M. First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.. in Plant Disease. 2013;97(11):1504-1504.
doi:10.1094/PDIS-03-13-0267-PDN .
Popović, Tatjana, Ivanović, Žarko, Živković, Svetlana, Trkulja, Nenad, Ignjatov, Maja, "First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia." in Plant Disease, 97, no. 11 (2013):1504-1504,
https://doi.org/10.1094/PDIS-03-13-0267-PDN . .
12
3
5

Identification of Colletotrichum acutatum from nectarine fruit

Živković, Svetlana; Gavrilović, Veljko; Oro, Violeta; Pfaf-Dolovac, Erika; Stošić, Stefan; Kuzmanović, Slobodan; Ivanović, Žarko

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Oro, Violeta
AU  - Pfaf-Dolovac, Erika
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/246
AB  - Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum.
AB  - Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Identification of Colletotrichum acutatum from nectarine fruit
T1  - Identifikacija Colletotrichum acutatum sa ploda nektarine
EP  - 138
IS  - 3
SP  - 130
VL  - 63
ER  - 
@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Oro, Violeta and Pfaf-Dolovac, Erika and Stošić, Stefan and Kuzmanović, Slobodan and Ivanović, Žarko",
year = "2012",
abstract = "Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum., Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Identification of Colletotrichum acutatum from nectarine fruit, Identifikacija Colletotrichum acutatum sa ploda nektarine",
pages = "138-130",
number = "3",
volume = "63"
}
Živković, S., Gavrilović, V., Oro, V., Pfaf-Dolovac, E., Stošić, S., Kuzmanović, S.,& Ivanović, Ž.. (2012). Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 130-138.
Živković S, Gavrilović V, Oro V, Pfaf-Dolovac E, Stošić S, Kuzmanović S, Ivanović Ž. Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja. 2012;63(3):130-138..
Živković, Svetlana, Gavrilović, Veljko, Oro, Violeta, Pfaf-Dolovac, Erika, Stošić, Stefan, Kuzmanović, Slobodan, Ivanović, Žarko, "Identification of Colletotrichum acutatum from nectarine fruit" in Zaštita bilja, 63, no. 3 (2012):130-138.

Pseudomonas syringae: Pathogen of sweet cherry in Serbia

Gavrilović, Veljko; Živković, Svetlana; Dolovac, Nenad; Trkulja, Nenad; Pfaf-Dolovac, Erika; Popović, Tatjana; Ivanović, Žarko

(Institute of Pesticides and Environmental Protection, 2012)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/224
AB  - Characteristics of pathogenic Pseudomonas bacterial strains isolated from cherry in Serbia are presented in the article. Two types of symptoms were observed on cherry trees at few localities with intensive production in Serbia (Belgrade, Čačak, Topola, Šabac, Novi Sad). The first symptom is bud necrosis and the second bacterial canker of cherry branch. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains were levan and HR positive, while negative results were recorded for oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Based on pathogenicity tests and differential GATT tests, investigated strains were divided in two distinct groups: the first group consisted of strains isolated from necrotic cherry branch which caused necrosis on artificially inoculated cherry, pear and lemon fruits, syringae leaves and bean pods, were gelatin and aesculin positive, and tyrosinase and tartrate negative (typical characteristics of P.s. pv. syringae). Contrary, second group strains were isolated from necrotic cherry buds, showed negative results in mentioned pathogenicity tests, gelatin and aesculin tests were negative, while tyrosinase and tartrate were positive (typical characteristics of P.s. pv. morsprunorum). REP PCR analyses showed that strains isolated from necrotic cherry buds belong to P. s pv. morsprunorum compared to referent strain. In contrast, isolates obtained from necrotic cherry branches had unique fingerprint profiles but different from all reference strains. According to the obtained results it was concluded that both pathovars of P. syringae (syringae and morsprunorum) cause necrosis of cherry trees in Serbia.
AB  - U radu su prikazane patogene, bakteriološke odlike izolata bakterije Pseudomnas syringae poreklom iz trešnje, kao i njihova molekularna karakterizacija primenom REP-PCR metode. Dva tipa simptoma bolesti zapažena su u savremenim zasadima trešnje na području Beograda, Novog Sada, Topole i Šapca. Prvi tip simptoma se ispoljava u vidu nekroze cvetnih i lisnih pupoljaka trešnje, a drugi u vidu nekroze višegodišnjih grana. Iz nekrotičnog tkiva su izolovane Gram negativne bakterije , koje fluoresciraju na King podlozi B, a glukozu metabolišu isključivo u aerobnim uslovima; bakterija stvara levan i prouzrokuje HR duvana ali ne stvara oksidazu, pektinazu u arginin dehidrolazu (LOPAT +---+) što su odlike karakteristične za bakteriju P. syringae. U pogledu patogenosti izolovanih sojeva, kao i njihovih diferencijalnih biohemijskih testova (GATT) proučavani izolati su svrstani u dve jasno izdiferencirane grupe. Prvu čine sojevi izolovani iz nekrozom zahavćenih grana trešnje, koji prouzrokuju nekrozu inokulisanih plodova trešnje, kruške i limuna, listova jorgovana i mahuna boranije. Ova grupa izolata hidrolizuje želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Pomenute patogene i diferencijalne biohemijske odlike su karakteristične za P.s. pv. syringae. Nasuprot njima, sojevi izolovani iz nekrotičnih pupoljaka trešnje negativno reaguju pri pomenutim testovima patogenosti, ne hidrolizuju želatin i eskulin, ali stvaraju tirozinazu i metabolišu tartarate, što su odlike P.s.pv. morsprunorum. REP-PCR analiza takođe ukazuje da sojevi iz nekrotičnih pupoljaka ispoljavaju identične karakteristike kao i referentni soj P.s. pv. morsprunorum. Primenom ovog metoda je takođe utvrđeno da su sojevi izolovani iz obolelih grana trešnje međusobno identični ali da se razlikuju od referentnih sojeva korišćenih u ovim istraživanjima. Na osnovu dobijenih rezultata, tokom ovih istraživanja zaključeno je da oba patogena varijeteta P. syringae patogena voćaka (pv. syrnigae i pv. morsprunorum) parazitiraju trešnju u našim agroekološkim uslovima.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pseudomonas syringae: Pathogen of sweet cherry in Serbia
T1  - Pseudomonas syringae - patogen trešnje u Srbiji
EP  - 149
IS  - 2
SP  - 141
VL  - 27
DO  - 10.2298/PIF1202141G
ER  - 
@article{
author = "Gavrilović, Veljko and Živković, Svetlana and Dolovac, Nenad and Trkulja, Nenad and Pfaf-Dolovac, Erika and Popović, Tatjana and Ivanović, Žarko",
year = "2012",
abstract = "Characteristics of pathogenic Pseudomonas bacterial strains isolated from cherry in Serbia are presented in the article. Two types of symptoms were observed on cherry trees at few localities with intensive production in Serbia (Belgrade, Čačak, Topola, Šabac, Novi Sad). The first symptom is bud necrosis and the second bacterial canker of cherry branch. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains were levan and HR positive, while negative results were recorded for oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Based on pathogenicity tests and differential GATT tests, investigated strains were divided in two distinct groups: the first group consisted of strains isolated from necrotic cherry branch which caused necrosis on artificially inoculated cherry, pear and lemon fruits, syringae leaves and bean pods, were gelatin and aesculin positive, and tyrosinase and tartrate negative (typical characteristics of P.s. pv. syringae). Contrary, second group strains were isolated from necrotic cherry buds, showed negative results in mentioned pathogenicity tests, gelatin and aesculin tests were negative, while tyrosinase and tartrate were positive (typical characteristics of P.s. pv. morsprunorum). REP PCR analyses showed that strains isolated from necrotic cherry buds belong to P. s pv. morsprunorum compared to referent strain. In contrast, isolates obtained from necrotic cherry branches had unique fingerprint profiles but different from all reference strains. According to the obtained results it was concluded that both pathovars of P. syringae (syringae and morsprunorum) cause necrosis of cherry trees in Serbia., U radu su prikazane patogene, bakteriološke odlike izolata bakterije Pseudomnas syringae poreklom iz trešnje, kao i njihova molekularna karakterizacija primenom REP-PCR metode. Dva tipa simptoma bolesti zapažena su u savremenim zasadima trešnje na području Beograda, Novog Sada, Topole i Šapca. Prvi tip simptoma se ispoljava u vidu nekroze cvetnih i lisnih pupoljaka trešnje, a drugi u vidu nekroze višegodišnjih grana. Iz nekrotičnog tkiva su izolovane Gram negativne bakterije , koje fluoresciraju na King podlozi B, a glukozu metabolišu isključivo u aerobnim uslovima; bakterija stvara levan i prouzrokuje HR duvana ali ne stvara oksidazu, pektinazu u arginin dehidrolazu (LOPAT +---+) što su odlike karakteristične za bakteriju P. syringae. U pogledu patogenosti izolovanih sojeva, kao i njihovih diferencijalnih biohemijskih testova (GATT) proučavani izolati su svrstani u dve jasno izdiferencirane grupe. Prvu čine sojevi izolovani iz nekrozom zahavćenih grana trešnje, koji prouzrokuju nekrozu inokulisanih plodova trešnje, kruške i limuna, listova jorgovana i mahuna boranije. Ova grupa izolata hidrolizuje želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Pomenute patogene i diferencijalne biohemijske odlike su karakteristične za P.s. pv. syringae. Nasuprot njima, sojevi izolovani iz nekrotičnih pupoljaka trešnje negativno reaguju pri pomenutim testovima patogenosti, ne hidrolizuju želatin i eskulin, ali stvaraju tirozinazu i metabolišu tartarate, što su odlike P.s.pv. morsprunorum. REP-PCR analiza takođe ukazuje da sojevi iz nekrotičnih pupoljaka ispoljavaju identične karakteristike kao i referentni soj P.s. pv. morsprunorum. Primenom ovog metoda je takođe utvrđeno da su sojevi izolovani iz obolelih grana trešnje međusobno identični ali da se razlikuju od referentnih sojeva korišćenih u ovim istraživanjima. Na osnovu dobijenih rezultata, tokom ovih istraživanja zaključeno je da oba patogena varijeteta P. syringae patogena voćaka (pv. syrnigae i pv. morsprunorum) parazitiraju trešnju u našim agroekološkim uslovima.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pseudomonas syringae: Pathogen of sweet cherry in Serbia, Pseudomonas syringae - patogen trešnje u Srbiji",
pages = "149-141",
number = "2",
volume = "27",
doi = "10.2298/PIF1202141G"
}
Gavrilović, V., Živković, S., Dolovac, N., Trkulja, N., Pfaf-Dolovac, E., Popović, T.,& Ivanović, Ž.. (2012). Pseudomonas syringae: Pathogen of sweet cherry in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 27(2), 141-149.
https://doi.org/10.2298/PIF1202141G
Gavrilović V, Živković S, Dolovac N, Trkulja N, Pfaf-Dolovac E, Popović T, Ivanović Ž. Pseudomonas syringae: Pathogen of sweet cherry in Serbia. in Pesticidi i fitomedicina. 2012;27(2):141-149.
doi:10.2298/PIF1202141G .
Gavrilović, Veljko, Živković, Svetlana, Dolovac, Nenad, Trkulja, Nenad, Pfaf-Dolovac, Erika, Popović, Tatjana, Ivanović, Žarko, "Pseudomonas syringae: Pathogen of sweet cherry in Serbia" in Pesticidi i fitomedicina, 27, no. 2 (2012):141-149,
https://doi.org/10.2298/PIF1202141G . .
3

Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits

Milosavljević, Anja; Stevanović, Miloš; Popović, Tatjana; Đukanović, Lana; Živković, Svetlana; Mitrović, Milana; Trkulja, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Stevanović, Miloš
AU  - Popović, Tatjana
AU  - Đukanović, Lana
AU  - Živković, Svetlana
AU  - Mitrović, Milana
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/240
AB  - Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium.
AB  - Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits
T1  - Morfološke i odgajivačke karakteristike izolata Monilinia laxa sa koštičavih voćaka
EP  - 158
IS  - 3
SP  - 148
VL  - 63
ER  - 
@article{
author = "Milosavljević, Anja and Stevanović, Miloš and Popović, Tatjana and Đukanović, Lana and Živković, Svetlana and Mitrović, Milana and Trkulja, Nenad",
year = "2012",
abstract = "Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium., Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits, Morfološke i odgajivačke karakteristike izolata Monilinia laxa sa koštičavih voćaka",
pages = "158-148",
number = "3",
volume = "63"
}
Milosavljević, A., Stevanović, M., Popović, T., Đukanović, L., Živković, S., Mitrović, M.,& Trkulja, N.. (2012). Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 148-158.
Milosavljević A, Stevanović M, Popović T, Đukanović L, Živković S, Mitrović M, Trkulja N. Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits. in Zaštita bilja. 2012;63(3):148-158..
Milosavljević, Anja, Stevanović, Miloš, Popović, Tatjana, Đukanović, Lana, Živković, Svetlana, Mitrović, Milana, Trkulja, Nenad, "Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits" in Zaštita bilja, 63, no. 3 (2012):148-158.

Morphology of two new Globodera rostochiensis populations from Serbia

Oro, Violeta; Živković, Svetlana; Dolovac, Nenad; Kuzmanović, Slobodan; Ivanović, Žarko

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Oro, Violeta
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
AU  - Kuzmanović, Slobodan
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/244
AB  - In concordance with phytosanitary regulations and the Program of continuous monitoring of quarantine and economically harmful nematodes that are intensively conducted every year, the presence of these organisms is determined in the territory of the Republic of Serbia and in the samples of plant material. Globodera pallida and G.rostochiensis are the most important parasites of potato in our country and their presence is monitored since 2005 and 2000 respectively. Morphology, i.e. examination of morphological characters of different populations of potato cyst nematodes determines their mutual similarities (differences) within species, between species and the similarity with the type populations. This paper presents the morphological descriptions of cysts and second stage larvae of two populations of G.rostochiensis found in localities Rujevac and Tabanovici in 2012.
AB  - U skladu sa Programom stalnog praćenja karantinskih i ekonomski štetnih nematoda koji se intenzivno sprovodi svake godine kao i fitosanitarnom regulativom, utvrđuje se prisustvo ovih organizama na teritoriji Republike Srbije i u uzorcima biljnog materijala. Globodera pallida i G. rostochiensis su najznačajniji paraziti krompira kod nas a njihovo prisustvo se prati od 2005. odnosno 2000. god. Morfologija odnosno ispitivanje morfoloških karakteristika različitih populacija cistolikih nematoda krompira daje podatke o njihovoj međusobnoj sličnosti (razlikama) unutar vrsta, između vrsta i sličnost sa tipskim populacijama. U radu su dati morfološki opisi cisti i larvi drugog stepena dve populacije G. rostochiensis pronadjene na lokalitetima Rujevac i Tabanovići u 2012. godini.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphology of two new Globodera rostochiensis populations from Serbia
T1  - Morfologija dve nove populacije Globodera rostochiensis iz Srbije
EP  - 129
IS  - 3
SP  - 123
VL  - 63
ER  - 
@article{
author = "Oro, Violeta and Živković, Svetlana and Dolovac, Nenad and Kuzmanović, Slobodan and Ivanović, Žarko",
year = "2012",
abstract = "In concordance with phytosanitary regulations and the Program of continuous monitoring of quarantine and economically harmful nematodes that are intensively conducted every year, the presence of these organisms is determined in the territory of the Republic of Serbia and in the samples of plant material. Globodera pallida and G.rostochiensis are the most important parasites of potato in our country and their presence is monitored since 2005 and 2000 respectively. Morphology, i.e. examination of morphological characters of different populations of potato cyst nematodes determines their mutual similarities (differences) within species, between species and the similarity with the type populations. This paper presents the morphological descriptions of cysts and second stage larvae of two populations of G.rostochiensis found in localities Rujevac and Tabanovici in 2012., U skladu sa Programom stalnog praćenja karantinskih i ekonomski štetnih nematoda koji se intenzivno sprovodi svake godine kao i fitosanitarnom regulativom, utvrđuje se prisustvo ovih organizama na teritoriji Republike Srbije i u uzorcima biljnog materijala. Globodera pallida i G. rostochiensis su najznačajniji paraziti krompira kod nas a njihovo prisustvo se prati od 2005. odnosno 2000. god. Morfologija odnosno ispitivanje morfoloških karakteristika različitih populacija cistolikih nematoda krompira daje podatke o njihovoj međusobnoj sličnosti (razlikama) unutar vrsta, između vrsta i sličnost sa tipskim populacijama. U radu su dati morfološki opisi cisti i larvi drugog stepena dve populacije G. rostochiensis pronadjene na lokalitetima Rujevac i Tabanovići u 2012. godini.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphology of two new Globodera rostochiensis populations from Serbia, Morfologija dve nove populacije Globodera rostochiensis iz Srbije",
pages = "129-123",
number = "3",
volume = "63"
}
Oro, V., Živković, S., Dolovac, N., Kuzmanović, S.,& Ivanović, Ž.. (2012). Morphology of two new Globodera rostochiensis populations from Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 123-129.
Oro V, Živković S, Dolovac N, Kuzmanović S, Ivanović Ž. Morphology of two new Globodera rostochiensis populations from Serbia. in Zaštita bilja. 2012;63(3):123-129..
Oro, Violeta, Živković, Svetlana, Dolovac, Nenad, Kuzmanović, Slobodan, Ivanović, Žarko, "Morphology of two new Globodera rostochiensis populations from Serbia" in Zaštita bilja, 63, no. 3 (2012):123-129.

Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria

Delić, Dušica; Stajković-Srbinović, Olivera; Živković, Svetlana; Protić, Nada; Rasulić, Nataša; Kuzmanović, Đorđe; Simić, Aleksandar

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Delić, Dušica
AU  - Stajković-Srbinović, Olivera
AU  - Živković, Svetlana
AU  - Protić, Nada
AU  - Rasulić, Nataša
AU  - Kuzmanović, Đorđe
AU  - Simić, Aleksandar
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/243
AB  - The object of this study was to evaluate the possible PGPR effects of ryegrass inoculation with Pseudomonas sp. strains as well as its coinoculation with Rhizobium trifolii on the yield and quality of Italian ryegrassm, with the aim to select effective strains as biofertilizer. The inoculation effects of PGPR on the yield of Italian Ryegrass cultivar K-29t were examined in pot experiment under greenhouse conditions. The experiment was designed with 3 inoculated treatments with 3 replications in completely randomised system. Inoculation of ryegrass with two Pseudomonas sp. strains LG and L1K alone as well as strain coinoculation with strains L1K and Rhizobium trfolii 459 represented treatments which were compared with control uninoculated treatment-Ø. The response of plant to the inoculation and coinoculation was positive in comparison to uninoculated plants. Results showed a significant positive influence of coinoculation with strains R. trifilii 459 and Pseudomonas sp. LG as well as Pseudomonas sp. L1K alone on shoot yield, N and P contents of Italian ryegrass. Results indicated that Pseudomonas sp. strains L1K and LG alone and in coinoculation with rhizobial strains can be investigated in further researches as potential agent of biofertilizer for plant growth promotion of Italian ryerass.
AB  - U eksperimentu u sudovima ispitivan je PGPR efekat sojeva Pseudomonas sp. kao i koinokulacija sa Rhizobium trifolii 459 na prinos i kvalitet italijanskog ljulja, Lolium multiflorum Lam. kroz prvi i drugi otkos a u cilju selekcije efikasnih sojeva kao biofertilizatora. Kao biljka domaćin, korišćena je sorta italijanskog ljulja K-29. Eksperiment se sastojao od tri tretmana sa inokulacijom i jednim kontrolnim neinokulisanim tretmanom u 3 ponavljanja u kompletno randomiziranom sistemu. Uticaj inokulacije italijanskog ljulja sa pojedinačnim sojevima LG i L1K kao i koinokulacije sa L1K i Rhizobium trfolii 459 ocenjen je kroz analizu određenih parametara (suva nadzemna masa, ukupan sadržaj N i P u suvoj nadzemnoj masi) koji su poređeni sa kontrolnim neinokulisanim tretmanom. Odgovor biljaka na inokulaciju i koinokulaciju je bio pozitivan u poređenju sa kontrolom. Prema rezultatima, koinokulacija ljulja sojevima R. trifilii 459 and Pseudomonas sp. LG kao i inokulacija pojedinačnim sojevima LG i L1K su uticali na značajno povečanje prinosa suve nadzemne mase u oba otkosa ali i sadržaja N i P u nadzemnom delu biljke. Rezultati su ukazali da sojevi Pseudomionasa samostalno ili u inokulaciji sa sojem R. trifolii imaju potencijal za unapređenje rasta italijanskog ljulja i da predstavljaju potencijalne agense biofertilizatora.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria
T1  - Unapređenje rasta italijanskog ljulja (Lolium multiflorum lam.) primenom PGP Rizobakterija
EP  - 99
IS  - 2
SP  - 93
VL  - 63
ER  - 
@article{
author = "Delić, Dušica and Stajković-Srbinović, Olivera and Živković, Svetlana and Protić, Nada and Rasulić, Nataša and Kuzmanović, Đorđe and Simić, Aleksandar",
year = "2012",
abstract = "The object of this study was to evaluate the possible PGPR effects of ryegrass inoculation with Pseudomonas sp. strains as well as its coinoculation with Rhizobium trifolii on the yield and quality of Italian ryegrassm, with the aim to select effective strains as biofertilizer. The inoculation effects of PGPR on the yield of Italian Ryegrass cultivar K-29t were examined in pot experiment under greenhouse conditions. The experiment was designed with 3 inoculated treatments with 3 replications in completely randomised system. Inoculation of ryegrass with two Pseudomonas sp. strains LG and L1K alone as well as strain coinoculation with strains L1K and Rhizobium trfolii 459 represented treatments which were compared with control uninoculated treatment-Ø. The response of plant to the inoculation and coinoculation was positive in comparison to uninoculated plants. Results showed a significant positive influence of coinoculation with strains R. trifilii 459 and Pseudomonas sp. LG as well as Pseudomonas sp. L1K alone on shoot yield, N and P contents of Italian ryegrass. Results indicated that Pseudomonas sp. strains L1K and LG alone and in coinoculation with rhizobial strains can be investigated in further researches as potential agent of biofertilizer for plant growth promotion of Italian ryerass., U eksperimentu u sudovima ispitivan je PGPR efekat sojeva Pseudomonas sp. kao i koinokulacija sa Rhizobium trifolii 459 na prinos i kvalitet italijanskog ljulja, Lolium multiflorum Lam. kroz prvi i drugi otkos a u cilju selekcije efikasnih sojeva kao biofertilizatora. Kao biljka domaćin, korišćena je sorta italijanskog ljulja K-29. Eksperiment se sastojao od tri tretmana sa inokulacijom i jednim kontrolnim neinokulisanim tretmanom u 3 ponavljanja u kompletno randomiziranom sistemu. Uticaj inokulacije italijanskog ljulja sa pojedinačnim sojevima LG i L1K kao i koinokulacije sa L1K i Rhizobium trfolii 459 ocenjen je kroz analizu određenih parametara (suva nadzemna masa, ukupan sadržaj N i P u suvoj nadzemnoj masi) koji su poređeni sa kontrolnim neinokulisanim tretmanom. Odgovor biljaka na inokulaciju i koinokulaciju je bio pozitivan u poređenju sa kontrolom. Prema rezultatima, koinokulacija ljulja sojevima R. trifilii 459 and Pseudomonas sp. LG kao i inokulacija pojedinačnim sojevima LG i L1K su uticali na značajno povečanje prinosa suve nadzemne mase u oba otkosa ali i sadržaja N i P u nadzemnom delu biljke. Rezultati su ukazali da sojevi Pseudomionasa samostalno ili u inokulaciji sa sojem R. trifolii imaju potencijal za unapređenje rasta italijanskog ljulja i da predstavljaju potencijalne agense biofertilizatora.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria, Unapređenje rasta italijanskog ljulja (Lolium multiflorum lam.) primenom PGP Rizobakterija",
pages = "99-93",
number = "2",
volume = "63"
}
Delić, D., Stajković-Srbinović, O., Živković, S., Protić, N., Rasulić, N., Kuzmanović, Đ.,& Simić, A.. (2012). Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(2), 93-99.
Delić D, Stajković-Srbinović O, Živković S, Protić N, Rasulić N, Kuzmanović Đ, Simić A. Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria. in Zaštita bilja. 2012;63(2):93-99..
Delić, Dušica, Stajković-Srbinović, Olivera, Živković, Svetlana, Protić, Nada, Rasulić, Nataša, Kuzmanović, Đorđe, Simić, Aleksandar, "Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria" in Zaštita bilja, 63, no. 2 (2012):93-99.

Etiology of diseases of some medicinal plants in plantation in Serbia

Pavlović, Snežana; Stojanović, Saša; Kuzmanović, Slobodan; Starović, Mira; Živković, Svetlana; Dolovac, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Stojanović, Saša
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/238
AB  - Marshmallow, coneflower, St. John's wort and sage, which grown in plantation, were the hosts of numerous fungi species that cause a variety of symptoms. A total of 34 species from 22 genera were determined. Some species pathogen on only one host (Puccinia malvacearum on marshmallow, Diaporthe eres and Seimatosporium hypericum on St. John's wort), while others were present at all examined hosts (species of the genus Fusarium are present in all examined hosts). The most abundant species in the seed was Alternaria alternate. The percentage of contaminated seeds ranged from 5% to 69%. Fusaium species were isolated from 2.5% to 29% of seeds, depending on the host and year. From the root of the studied medicinal plants seven species of Fusarium (F.graminearum, F. oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans and F.verticilioides) and one of the genus Sclerotinia (S.sclerotiorum) were isolated. Thirteen fungi species belonging to 10 genera were isolated from stem, leaves and flower of medicinal plants.
AB  - Plantažno gajeni beli slez, ehinacee, kantarion i žalfija su domaćini brojnih vrsta gljiva koje prouzrokuju različite simptome. Ukupno je determinisano 34 vrsta iz 22 roda. Neke vrste parazitiraju samo jednog domaćina (Puccinia malvacearum na belom slezu, Diaporthe eres i Seimatosporium hypericum na kantarionu), dok druge ispoljavaju izrazitu polifagnost (vrste iz roda Fusarium prisutne su na svim ispitivanim domaćinima). Najzastupljenija vrsta na semenu bila je Alternaria alternata, koja je uvek prisutna na semenu u visokom procentu, a pojednih godina i preko 60 %. Procenat kontaminiranog semana kretao se od 5% do 69%. godini. U proseku procenat zaraženog semena sa vrstama roda Fusaium bio 17,2% i kretao se od 2,5% do 29% u zavisnosti od domaćina i godine. Na korenu ispitivanih lekovitih biljaka izolovano je sedam vrsta gljiva iz roda Fusarium (F.graminearum, F.oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans i F verticilioides) i jedna iz roda Sclerotinia (S.sclerotiorum). Sa nadzemnih organa izolovano je 13 vrsta gljiva iz 10 rodova.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Etiology of diseases of some medicinal plants in plantation in Serbia
T1  - Etiologija oboljenja važnijih plantažno gajenih lekovitih biljaka u Srbiji
EP  - 241
IS  - 4
SP  - 224
VL  - 63
ER  - 
@article{
author = "Pavlović, Snežana and Stojanović, Saša and Kuzmanović, Slobodan and Starović, Mira and Živković, Svetlana and Dolovac, Nenad",
year = "2012",
abstract = "Marshmallow, coneflower, St. John's wort and sage, which grown in plantation, were the hosts of numerous fungi species that cause a variety of symptoms. A total of 34 species from 22 genera were determined. Some species pathogen on only one host (Puccinia malvacearum on marshmallow, Diaporthe eres and Seimatosporium hypericum on St. John's wort), while others were present at all examined hosts (species of the genus Fusarium are present in all examined hosts). The most abundant species in the seed was Alternaria alternate. The percentage of contaminated seeds ranged from 5% to 69%. Fusaium species were isolated from 2.5% to 29% of seeds, depending on the host and year. From the root of the studied medicinal plants seven species of Fusarium (F.graminearum, F. oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans and F.verticilioides) and one of the genus Sclerotinia (S.sclerotiorum) were isolated. Thirteen fungi species belonging to 10 genera were isolated from stem, leaves and flower of medicinal plants., Plantažno gajeni beli slez, ehinacee, kantarion i žalfija su domaćini brojnih vrsta gljiva koje prouzrokuju različite simptome. Ukupno je determinisano 34 vrsta iz 22 roda. Neke vrste parazitiraju samo jednog domaćina (Puccinia malvacearum na belom slezu, Diaporthe eres i Seimatosporium hypericum na kantarionu), dok druge ispoljavaju izrazitu polifagnost (vrste iz roda Fusarium prisutne su na svim ispitivanim domaćinima). Najzastupljenija vrsta na semenu bila je Alternaria alternata, koja je uvek prisutna na semenu u visokom procentu, a pojednih godina i preko 60 %. Procenat kontaminiranog semana kretao se od 5% do 69%. godini. U proseku procenat zaraženog semena sa vrstama roda Fusaium bio 17,2% i kretao se od 2,5% do 29% u zavisnosti od domaćina i godine. Na korenu ispitivanih lekovitih biljaka izolovano je sedam vrsta gljiva iz roda Fusarium (F.graminearum, F.oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans i F verticilioides) i jedna iz roda Sclerotinia (S.sclerotiorum). Sa nadzemnih organa izolovano je 13 vrsta gljiva iz 10 rodova.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Etiology of diseases of some medicinal plants in plantation in Serbia, Etiologija oboljenja važnijih plantažno gajenih lekovitih biljaka u Srbiji",
pages = "241-224",
number = "4",
volume = "63"
}
Pavlović, S., Stojanović, S., Kuzmanović, S., Starović, M., Živković, S.,& Dolovac, N.. (2012). Etiology of diseases of some medicinal plants in plantation in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(4), 224-241.
Pavlović S, Stojanović S, Kuzmanović S, Starović M, Živković S, Dolovac N. Etiology of diseases of some medicinal plants in plantation in Serbia. in Zaštita bilja. 2012;63(4):224-241..
Pavlović, Snežana, Stojanović, Saša, Kuzmanović, Slobodan, Starović, Mira, Živković, Svetlana, Dolovac, Nenad, "Etiology of diseases of some medicinal plants in plantation in Serbia" in Zaštita bilja, 63, no. 4 (2012):224-241.

Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium

Popović, Tatjana; Ignjatov, Maja; Jošić, Dragana; Starović, Mira; Živković, Svetlana; Aleksić, Goran; Trkulja, Nenad

(Institute of field and vegetable crops, Novi Sad, 2012)

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/230
AB  - Bean production is threaten by phytopathogenic bacteria causing agents of blights, Xanthomonas axonopodis pv. phaseoli (Xap) and Pseudomonas savastanoi pv. phaseolicola (Psp). Since there is no satisfactory chemical control for the disease, the recommended measures are preventive and include use of healthy seed, crop rotation, deep plowing and use of resistant cultivars. In this work we involved a detection method for isolation of Xap and Psp from bean seed to semi-selective medium Milk Agar Tween (MT). On this medium, Xap formed yellow, mucoid and convex colonies with two hydrolysis zones (less milk and more enlightened), and Psp formed whitish-cream, flat and round colonies. The identification of Xap and Psp was confirmed using the ELISA and PCR. Due to its selectivity, easy preparation and possibility of simultaneous detection of bacteria Xap and Psp, MT medium can be recommended for routine test of seed health for local seed or seed from import.
AB  - Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium
T1  - Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge
EP  - 38
IS  - 1
SP  - 34
VL  - 49
DO  - 10.5937/ratpov49-1126
ER  - 
@article{
author = "Popović, Tatjana and Ignjatov, Maja and Jošić, Dragana and Starović, Mira and Živković, Svetlana and Aleksić, Goran and Trkulja, Nenad",
year = "2012",
abstract = "Bean production is threaten by phytopathogenic bacteria causing agents of blights, Xanthomonas axonopodis pv. phaseoli (Xap) and Pseudomonas savastanoi pv. phaseolicola (Psp). Since there is no satisfactory chemical control for the disease, the recommended measures are preventive and include use of healthy seed, crop rotation, deep plowing and use of resistant cultivars. In this work we involved a detection method for isolation of Xap and Psp from bean seed to semi-selective medium Milk Agar Tween (MT). On this medium, Xap formed yellow, mucoid and convex colonies with two hydrolysis zones (less milk and more enlightened), and Psp formed whitish-cream, flat and round colonies. The identification of Xap and Psp was confirmed using the ELISA and PCR. Due to its selectivity, easy preparation and possibility of simultaneous detection of bacteria Xap and Psp, MT medium can be recommended for routine test of seed health for local seed or seed from import., Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium, Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge",
pages = "38-34",
number = "1",
volume = "49",
doi = "10.5937/ratpov49-1126"
}
Popović, T., Ignjatov, M., Jošić, D., Starović, M., Živković, S., Aleksić, G.,& Trkulja, N.. (2012). Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 49(1), 34-38.
https://doi.org/10.5937/ratpov49-1126
Popović T, Ignjatov M, Jošić D, Starović M, Živković S, Aleksić G, Trkulja N. Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium. in Ratarstvo i povrtarstvo. 2012;49(1):34-38.
doi:10.5937/ratpov49-1126 .
Popović, Tatjana, Ignjatov, Maja, Jošić, Dragana, Starović, Mira, Živković, Svetlana, Aleksić, Goran, Trkulja, Nenad, "Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium" in Ratarstvo i povrtarstvo, 49, no. 1 (2012):34-38,
https://doi.org/10.5937/ratpov49-1126 . .
1

Pathogenicity of isolates of Colletotrichum spp.: The causal agents of anthracnose

Živković, Svetlana; Dolovac, Nenad; Popović, Tatjana; Stojanović, Saša

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
AU  - Popović, Tatjana
AU  - Stojanović, Saša
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/205
AB  - The pathogenic characteristics of 20 isolates of Colletotrichum spp. originating from pear, apple, sour cherry and tomato fruits, as well as reference strains of C. acutatum (CBS 294.67) and C. gloeosporioides (CBS 516.97) are presented in this paper. In the studies of host range of isolates of Colletotrichum spp. were included 17 plant species. Nine days after artificial inoculation all tested isolates were caused anthracnose lesion on fruits of apple, pear, peach, apricot, sour cherry, sweet cherry, plum, strawberry, grape, tomato, pepper, eggplant, cucumber, zucchini, and green beans. Isolates of Colletotrichum spp. originating from the same host showed some differences in the degree of aggressiveness, which can be interpreted as the genetic variability of populations. The results of one-way analysis of variance indicate that the pathogenicity of isolates of Colletotrichum spp. is statistically significantly different on immature and mature fruits of pear, apple, sour cherry and tomato. Low to moderate sensitivity of immature fruits was confirmed by comparison of the degree of necrosis. Mature fruits were manifested a strong sensitivity to all tested isolates of Colletotrichum spp.
AB  - U radu su prikazane patogene karakteristike 20 izolata Colletotrichum spp. poreklom sa plodova kruške, jabuke, višnje i paradajza, kao i referentnih sojeva C. acutatum (CBS 294.67) i C. gloeosporioides (CBS 516.97). U proučavanja kruga domaćina izolata Colletotrichum spp. uključeno je 17 biljnih vrsta. Devet dana nakon veštačkih inokulacija svi testirani izolati prouzrokovali su antraknozne lezije na plodu jabuke, kruške, kajsije, trešnje, višnje, šljive, jagode, grožđa, paradajza, paprike, plavog patlidžana, krastavca, tikvice i boranije. Izolati Colletotrichum spp. poreklom sa istog domaćina pokazuju izvesne razlike u stepenu agresivnosti, što se može tumačiti genetskom varijabilnošću populacija. Rezultati jednofaktorijalne analize varijanse ukazuju na statistički značajne razlike u patogenosti izolata Colletotrichum spp. na nezrelim i zrelim plodovima kruške, jabuke, višnje i paradajza. Poređenjem stepena nekroze utvrđena je slaba do umerena osetljivost nezrelih plodova. Zreli plodovi su manifestovali jaku osetljivost prema svim testiranim izolatima Colletotrichum spp.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Pathogenicity of isolates of Colletotrichum spp.: The causal agents of anthracnose
T1  - Patogenost izolata Colletotrichum spp. - prouzrokovača antraknoze
EP  - 21
IS  - 1
SP  - 7
VL  - 63
ER  - 
@article{
author = "Živković, Svetlana and Dolovac, Nenad and Popović, Tatjana and Stojanović, Saša",
year = "2012",
abstract = "The pathogenic characteristics of 20 isolates of Colletotrichum spp. originating from pear, apple, sour cherry and tomato fruits, as well as reference strains of C. acutatum (CBS 294.67) and C. gloeosporioides (CBS 516.97) are presented in this paper. In the studies of host range of isolates of Colletotrichum spp. were included 17 plant species. Nine days after artificial inoculation all tested isolates were caused anthracnose lesion on fruits of apple, pear, peach, apricot, sour cherry, sweet cherry, plum, strawberry, grape, tomato, pepper, eggplant, cucumber, zucchini, and green beans. Isolates of Colletotrichum spp. originating from the same host showed some differences in the degree of aggressiveness, which can be interpreted as the genetic variability of populations. The results of one-way analysis of variance indicate that the pathogenicity of isolates of Colletotrichum spp. is statistically significantly different on immature and mature fruits of pear, apple, sour cherry and tomato. Low to moderate sensitivity of immature fruits was confirmed by comparison of the degree of necrosis. Mature fruits were manifested a strong sensitivity to all tested isolates of Colletotrichum spp., U radu su prikazane patogene karakteristike 20 izolata Colletotrichum spp. poreklom sa plodova kruške, jabuke, višnje i paradajza, kao i referentnih sojeva C. acutatum (CBS 294.67) i C. gloeosporioides (CBS 516.97). U proučavanja kruga domaćina izolata Colletotrichum spp. uključeno je 17 biljnih vrsta. Devet dana nakon veštačkih inokulacija svi testirani izolati prouzrokovali su antraknozne lezije na plodu jabuke, kruške, kajsije, trešnje, višnje, šljive, jagode, grožđa, paradajza, paprike, plavog patlidžana, krastavca, tikvice i boranije. Izolati Colletotrichum spp. poreklom sa istog domaćina pokazuju izvesne razlike u stepenu agresivnosti, što se može tumačiti genetskom varijabilnošću populacija. Rezultati jednofaktorijalne analize varijanse ukazuju na statistički značajne razlike u patogenosti izolata Colletotrichum spp. na nezrelim i zrelim plodovima kruške, jabuke, višnje i paradajza. Poređenjem stepena nekroze utvrđena je slaba do umerena osetljivost nezrelih plodova. Zreli plodovi su manifestovali jaku osetljivost prema svim testiranim izolatima Colletotrichum spp.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Pathogenicity of isolates of Colletotrichum spp.: The causal agents of anthracnose, Patogenost izolata Colletotrichum spp. - prouzrokovača antraknoze",
pages = "21-7",
number = "1",
volume = "63"
}
Živković, S., Dolovac, N., Popović, T.,& Stojanović, S.. (2012). Pathogenicity of isolates of Colletotrichum spp.: The causal agents of anthracnose. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(1), 7-21.
Živković S, Dolovac N, Popović T, Stojanović S. Pathogenicity of isolates of Colletotrichum spp.: The causal agents of anthracnose. in Zaštita bilja. 2012;63(1):7-21..
Živković, Svetlana, Dolovac, Nenad, Popović, Tatjana, Stojanović, Saša, "Pathogenicity of isolates of Colletotrichum spp.: The causal agents of anthracnose" in Zaštita bilja, 63, no. 1 (2012):7-21.