@conference{
author = "Kljajić, Petar and Milovanović, Predrag and Andrić, Goran and Pražić Golić, Marijana and Jovičić, Ivana and Milovac, Željko and Franeta, Filip",
year = "2019",
abstract = "Intensive use of synthetic insecticides, mostly pyrethroids and organophosphates, for control of pollen beetle, Brassicogethes aeneus (F.), a major pest in winter oilseed rape (OSR), is a priority concern regarding their increased potential for selection pressure, which leads to increasingly widespread resistance development. The objective of this study was to determine susceptibility levels of B. aeneus populations from Serbia to insecticides from different classes (organophosphates, pyrethroids and oxidiazines). Using IRAC Susceptibility Test Method No. 027, populations were sampled from fields with winter OSR crops in: Bačka Topola, Kikinda, Dobanovci and Novi Sad (Rimski šančevi 1 and Rimski šančevi 2). More than 300 specimens per population were collected during April 2019 and, before bioassays were set up, the imagoes of B. aeneus were stored at 5°C. Only imagoes which had good fitness were used in the bioassays. Insecticides based on lambda-cyhalothrin, cypermethrin, deltamethrin, chlorpyrifos + cypermethrin and indoxacarb were diluted in water + acetone mixture (5%+95%) and applied at 25 and 100% of their recommended label doses (RLD). Vials (5.5 cm x 2.5 cm, and 48 cm2 internal surfaces) were used in the lab trials and they were filled with 0.5 mL solution of each insecticide and then rotated until the deposits dried. Control vials contained only water + acetone mixture. Ten imagoes were added to each vial. Each test dose and insecticide was represented in three replicates and the trial was carried out at 22±2°C temperature, 60±5% RH and 16:8 LD photoperiod. Dead imagoes were counted after 24 h of exposure to each insecticide deposit. The results show that pollen beetles from all test populations were highly susceptible (caused 100% mortality) to 100% RLD of all insecticides, except Dobanovci and Bačka Topola populations, which were susceptible to deltamethrin and lambda-cyhalothrin, 97% and 93% mortality, respectively. Also, only chlorpyrifos + cypermethrin and indoxacarb 25% RLD caused 100% mortality of test populations, and all insecticides except deltamethrin in Rimski šančevi 2 population. The lowest susceptibility caused by deltamethrin 25% RLD was detected in Bačka Topola and Rimski šančevi 1 beetles, only 40% mortality, then Kikinda beetles, 27% mortality, and beetles from Rimski šančevi 2, no mortality. Based on IRAC classification, we inferred that all test populations of pollen beetles from Serbia belong to the second group, i.e. populations susceptible to the test insecticides.",
publisher = "Društvo za zaštitu bilja",
journal = "VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia",
title = "Susceptibility of Brassicogethes aeneus (f.) (Coleoptera: Nitidulidae) populations from Serbia to insecticides",
pages = "134"
}
