TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Jovanović, Gordana
AU  - Ivanović, Borko
AU  - Žeželj, Danica
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/746
AB  - Early blight is an economically important disease of potato worldwide. Understanding which fungal pathogens are the causal agents of early blight and their distribution on the same host is essential to finding the best strategy for the control of this disease. Previous studies have shown that Alternaria solani is the main early blight pathogen parasitizing potato. Here, we analyzed genetic and phenotypic diversity in isolates of Alternaria spp. covering all potato production areas in Serbia. We showed that the four species of Alternaria were found in areas with different distributions of the species. The occurrence of Alternaria spp. was studied by analyzing isolates from symptomatic potato leaves during multiyear sampling. In addition to Alternaria solani, we detected three more large-spored species identified as A. linariae (syn. A. tomatophila), A. protenta, and A. grandis that were involved in early blight disease on naturally infected potato leaves in Serbia. Differentiation of species was supported by phylogeny obtained from the DNA sequences of the GAPDH, calmodulin and Rpb2 genes. Our findings present a new perspective into the population structure of large-spored Alternaria species associated with early blight disease. Within the groups of large-spored Alternaria present in Serbia, evidence of A. protenta at high frequency reveals new insight into the contribution of Alternaria species in early blight disease. This work opens new perspectives for early blight management, while the distribution of different species on the same host suggests that the etiology of disease could depend on crop organization and the presence of other Alternaria hosts in close proximity to potato plants.
PB  - Frontiers Media
T2  - Frontiers in Microbiology
T1  - New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia
IS  - 856898
VL  - 13
DO  - 10.3389/fmicb.2022.856898
ER  - 

@article{
author = "Ivanović, Žarko and Blagojević, Jovana and Jovanović, Gordana and Ivanović, Borko and Žeželj, Danica",
year = "2022",
abstract = "Early blight is an economically important disease of potato worldwide. Understanding which fungal pathogens are the causal agents of early blight and their distribution on the same host is essential to finding the best strategy for the control of this disease. Previous studies have shown that Alternaria solani is the main early blight pathogen parasitizing potato. Here, we analyzed genetic and phenotypic diversity in isolates of Alternaria spp. covering all potato production areas in Serbia. We showed that the four species of Alternaria were found in areas with different distributions of the species. The occurrence of Alternaria spp. was studied by analyzing isolates from symptomatic potato leaves during multiyear sampling. In addition to Alternaria solani, we detected three more large-spored species identified as A. linariae (syn. A. tomatophila), A. protenta, and A. grandis that were involved in early blight disease on naturally infected potato leaves in Serbia. Differentiation of species was supported by phylogeny obtained from the DNA sequences of the GAPDH, calmodulin and Rpb2 genes. Our findings present a new perspective into the population structure of large-spored Alternaria species associated with early blight disease. Within the groups of large-spored Alternaria present in Serbia, evidence of A. protenta at high frequency reveals new insight into the contribution of Alternaria species in early blight disease. This work opens new perspectives for early blight management, while the distribution of different species on the same host suggests that the etiology of disease could depend on crop organization and the presence of other Alternaria hosts in close proximity to potato plants.",
publisher = "Frontiers Media",
journal = "Frontiers in Microbiology",
title = "New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia",
number = "856898",
volume = "13",
doi = "10.3389/fmicb.2022.856898"
}

Ivanović, Ž., Blagojević, J., Jovanović, G., Ivanović, B.,& Žeželj, D.. (2022). New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia. in Frontiers in Microbiology
Frontiers Media., 13(856898).
https://doi.org/10.3389/fmicb.2022.856898

Ivanović Ž, Blagojević J, Jovanović G, Ivanović B, Žeželj D. New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia. in Frontiers in Microbiology. 2022;13(856898).
doi:10.3389/fmicb.2022.856898 .

Ivanović, Žarko, Blagojević, Jovana, Jovanović, Gordana, Ivanović, Borko, Žeželj, Danica, "New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia" in Frontiers in Microbiology, 13, no. 856898 (2022),
https://doi.org/10.3389/fmicb.2022.856898 . .

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Vukojevic, Jelena
AU  - Ivanović, Borko
AU  - Ivanović, Žarko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/749
AB  - Leaf spot diseases caused by Alternaria species have been reported worldwide in plants in the Brassicaceae family. However, there is little information on Alternaria species causing diseases in horseradish. In the present study, 89 Alternaria spp. isolates from Armoracia rusticana, sampled from nine districts in Serbia, were characterized based on their morphology, physiology, and molecular markers. Morphological characterization and molecular analyses based on ITS, GAPDH, Alt a 1, and PM-ATP sequences identified three distinct species associated with the disease: Alternaria brassicae, A. brassicicola, and A. alternata. For all species, growth and sporulation rates at 0, 5, 10, 15, 20, 25, 30, 35, and 40°C showed a quadratic response, with A. alternata having the widest temperature optimum (20 to 30°C) while A. brassicicola had higher optimum temperatures (20 to 25°C) than A. brassicae (15 to 20°C). To gain a better understanding of the pathogenicity of these species, the influence of leaf age, host susceptibility, and ability to infect artificially wounded and nonwounded leaves were tested. The pathogenicity test identified A. brassicicola and A. brassicae as the main causal agents of horseradish leaf spot disease. Results indicated that young and intact leaves of horseradish and cabbage were less susceptible to infection and also suggested the potential for cross-infection between these two hosts. Haplotype networks showed haplotype uniformity for A. brassicae, two haplotype groups of A. brassicicola, and eight haplotype groups of A. alternata in Serbia and suggest the possible association of some haplotypes with the geographic area. This study is the first to investigate Alternaria leaf spot disease on A. rusticana in Serbia and is the first record of A. brassicicola on horseradish in this country.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia
EP  - 1389
IS  - 5
SP  - 1378
VL  - 104
DO  - 10.1094/pdis-02-19-0289-re
DO  - 0191-2917
ER  - 

@article{
author = "Blagojević, Jovana and Vukojevic, Jelena and Ivanović, Borko and Ivanović, Žarko",
year = "2020",
abstract = "Leaf spot diseases caused by Alternaria species have been reported worldwide in plants in the Brassicaceae family. However, there is little information on Alternaria species causing diseases in horseradish. In the present study, 89 Alternaria spp. isolates from Armoracia rusticana, sampled from nine districts in Serbia, were characterized based on their morphology, physiology, and molecular markers. Morphological characterization and molecular analyses based on ITS, GAPDH, Alt a 1, and PM-ATP sequences identified three distinct species associated with the disease: Alternaria brassicae, A. brassicicola, and A. alternata. For all species, growth and sporulation rates at 0, 5, 10, 15, 20, 25, 30, 35, and 40°C showed a quadratic response, with A. alternata having the widest temperature optimum (20 to 30°C) while A. brassicicola had higher optimum temperatures (20 to 25°C) than A. brassicae (15 to 20°C). To gain a better understanding of the pathogenicity of these species, the influence of leaf age, host susceptibility, and ability to infect artificially wounded and nonwounded leaves were tested. The pathogenicity test identified A. brassicicola and A. brassicae as the main causal agents of horseradish leaf spot disease. Results indicated that young and intact leaves of horseradish and cabbage were less susceptible to infection and also suggested the potential for cross-infection between these two hosts. Haplotype networks showed haplotype uniformity for A. brassicae, two haplotype groups of A. brassicicola, and eight haplotype groups of A. alternata in Serbia and suggest the possible association of some haplotypes with the geographic area. This study is the first to investigate Alternaria leaf spot disease on A. rusticana in Serbia and is the first record of A. brassicicola on horseradish in this country.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia",
pages = "1389-1378",
number = "5",
volume = "104",
doi = "10.1094/pdis-02-19-0289-re, 0191-2917"
}

Blagojević, J., Vukojevic, J., Ivanović, B.,& Ivanović, Ž.. (2020). Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia. in Plant Disease
American Phytopathological Society., 104(5), 1378-1389.
https://doi.org/10.1094/pdis-02-19-0289-re

Blagojević J, Vukojevic J, Ivanović B, Ivanović Ž. Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia. in Plant Disease. 2020;104(5):1378-1389.
doi:10.1094/pdis-02-19-0289-re .

Blagojević, Jovana, Vukojevic, Jelena, Ivanović, Borko, Ivanović, Žarko, "Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia" in Plant Disease, 104, no. 5 (2020):1378-1389,
https://doi.org/10.1094/pdis-02-19-0289-re . .