TY  - JOUR
AU  - Blagojević, Jovana
AU  - Vukojevic, Jelena
AU  - Ivanović, Borko
AU  - Ivanović, Žarko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/749
AB  - Leaf spot diseases caused by Alternaria species have been reported worldwide in plants in the Brassicaceae family. However, there is little information on Alternaria species causing diseases in horseradish. In the present study, 89 Alternaria spp. isolates from Armoracia rusticana, sampled from nine districts in Serbia, were characterized based on their morphology, physiology, and molecular markers. Morphological characterization and molecular analyses based on ITS, GAPDH, Alt a 1, and PM-ATP sequences identified three distinct species associated with the disease: Alternaria brassicae, A. brassicicola, and A. alternata. For all species, growth and sporulation rates at 0, 5, 10, 15, 20, 25, 30, 35, and 40°C showed a quadratic response, with A. alternata having the widest temperature optimum (20 to 30°C) while A. brassicicola had higher optimum temperatures (20 to 25°C) than A. brassicae (15 to 20°C). To gain a better understanding of the pathogenicity of these species, the influence of leaf age, host susceptibility, and ability to infect artificially wounded and nonwounded leaves were tested. The pathogenicity test identified A. brassicicola and A. brassicae as the main causal agents of horseradish leaf spot disease. Results indicated that young and intact leaves of horseradish and cabbage were less susceptible to infection and also suggested the potential for cross-infection between these two hosts. Haplotype networks showed haplotype uniformity for A. brassicae, two haplotype groups of A. brassicicola, and eight haplotype groups of A. alternata in Serbia and suggest the possible association of some haplotypes with the geographic area. This study is the first to investigate Alternaria leaf spot disease on A. rusticana in Serbia and is the first record of A. brassicicola on horseradish in this country.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia
EP  - 1389
IS  - 5
SP  - 1378
VL  - 104
DO  - 10.1094/pdis-02-19-0289-re
DO  - 0191-2917
ER  - 

@article{
author = "Blagojević, Jovana and Vukojevic, Jelena and Ivanović, Borko and Ivanović, Žarko",
year = "2020",
abstract = "Leaf spot diseases caused by Alternaria species have been reported worldwide in plants in the Brassicaceae family. However, there is little information on Alternaria species causing diseases in horseradish. In the present study, 89 Alternaria spp. isolates from Armoracia rusticana, sampled from nine districts in Serbia, were characterized based on their morphology, physiology, and molecular markers. Morphological characterization and molecular analyses based on ITS, GAPDH, Alt a 1, and PM-ATP sequences identified three distinct species associated with the disease: Alternaria brassicae, A. brassicicola, and A. alternata. For all species, growth and sporulation rates at 0, 5, 10, 15, 20, 25, 30, 35, and 40°C showed a quadratic response, with A. alternata having the widest temperature optimum (20 to 30°C) while A. brassicicola had higher optimum temperatures (20 to 25°C) than A. brassicae (15 to 20°C). To gain a better understanding of the pathogenicity of these species, the influence of leaf age, host susceptibility, and ability to infect artificially wounded and nonwounded leaves were tested. The pathogenicity test identified A. brassicicola and A. brassicae as the main causal agents of horseradish leaf spot disease. Results indicated that young and intact leaves of horseradish and cabbage were less susceptible to infection and also suggested the potential for cross-infection between these two hosts. Haplotype networks showed haplotype uniformity for A. brassicae, two haplotype groups of A. brassicicola, and eight haplotype groups of A. alternata in Serbia and suggest the possible association of some haplotypes with the geographic area. This study is the first to investigate Alternaria leaf spot disease on A. rusticana in Serbia and is the first record of A. brassicicola on horseradish in this country.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia",
pages = "1389-1378",
number = "5",
volume = "104",
doi = "10.1094/pdis-02-19-0289-re, 0191-2917"
}

Blagojević, J., Vukojevic, J., Ivanović, B.,& Ivanović, Ž.. (2020). Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia. in Plant Disease
American Phytopathological Society., 104(5), 1378-1389.
https://doi.org/10.1094/pdis-02-19-0289-re

Blagojević J, Vukojevic J, Ivanović B, Ivanović Ž. Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia. in Plant Disease. 2020;104(5):1378-1389.
doi:10.1094/pdis-02-19-0289-re .

Blagojević, Jovana, Vukojevic, Jelena, Ivanović, Borko, Ivanović, Žarko, "Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia" in Plant Disease, 104, no. 5 (2020):1378-1389,
https://doi.org/10.1094/pdis-02-19-0289-re . .