TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Krstić, Branka
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/643
AB  - Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RTPCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia
PB  - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
T2  - Spanish Journal of Agricultural Research
T1  - Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes
IS  - 4
VL  - 18
DO  - 10.5424/sjar/2020184-16244
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Lela and Vučurović, Ivan and Krstić, Branka",
year = "2020",
abstract = "Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RTPCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia",
publisher = "Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)",
journal = "Spanish Journal of Agricultural Research",
title = "Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes",
number = "4",
volume = "18",
doi = "10.5424/sjar/2020184-16244"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, L., Vučurović, I.,& Krstić, B.. (2020). Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes. in Spanish Journal of Agricultural Research
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)., 18(4).
https://doi.org/10.5424/sjar/2020184-16244

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić L, Vučurović I, Krstić B. Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes. in Spanish Journal of Agricultural Research. 2020;18(4).
doi:10.5424/sjar/2020184-16244 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Lela, Vučurović, Ivan, Krstić, Branka, "Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes" in Spanish Journal of Agricultural Research, 18, no. 4 (2020),
https://doi.org/10.5424/sjar/2020184-16244 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Danijela
AU  - Vucurović, Ivan
AU  - Krstić, Branka
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/594
AB  - A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia
EP  - 797
IS  - 3
SP  - 787
VL  - 102
DO  - 10.1007/s42161-020-00504-7
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Danijela and Vucurović, Ivan and Krstić, Branka",
year = "2020",
abstract = "A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia",
pages = "797-787",
number = "3",
volume = "102",
doi = "10.1007/s42161-020-00504-7"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, D., Vucurović, I.,& Krstić, B.. (2020). Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology
Springer, New York., 102(3), 787-797.
https://doi.org/10.1007/s42161-020-00504-7

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić D, Vucurović I, Krstić B. Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology. 2020;102(3):787-797.
doi:10.1007/s42161-020-00504-7 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Danijela, Vucurović, Ivan, Krstić, Branka, "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia" in Journal of Plant Pathology, 102, no. 3 (2020):787-797,
https://doi.org/10.1007/s42161-020-00504-7 . .