TY  - JOUR
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Menković, Jelena
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/715
AB  - During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.
PB  - APS Publications
T2  - PLANT DISEASE
T1  - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
IS  - 3
VL  - 107
DO  - 10.1094/PDIS-06-22-1425-RE
ER  - 

@article{
author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2023",
abstract = "During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.",
publisher = "APS Publications",
journal = "PLANT DISEASE",
title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia",
number = "3",
volume = "107",
doi = "10.1094/PDIS-06-22-1425-RE"
}

Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in PLANT DISEASE
APS Publications., 107(3).
https://doi.org/10.1094/PDIS-06-22-1425-RE

Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in PLANT DISEASE. 2023;107(3).
doi:10.1094/PDIS-06-22-1425-RE .

Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in PLANT DISEASE, 107, no. 3 (2023),
https://doi.org/10.1094/PDIS-06-22-1425-RE . .

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
American Phytopathological Society., 103(10), 2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Anđelka, Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/905
AB  - Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Differentiation of Pseudomonas syringae pathovars originating from stone fruits
EP  - 229
IS  - 3
SP  - 219
VL  - 27
DO  - 10.2298/PIF1203219G
ER  - 

@article{
author = "Gašić, Katarina and Prokić, Anđelka and Ivanović, Milan and Kuzmanović, Nemanja and Obradović, Aleksa",
year = "2012",
abstract = "Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Differentiation of Pseudomonas syringae pathovars originating from stone fruits",
pages = "229-219",
number = "3",
volume = "27",
doi = "10.2298/PIF1203219G"
}

Gašić, K., Prokić, A., Ivanović, M., Kuzmanović, N.,& Obradović, A.. (2012). Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 27(3), 219-229.
https://doi.org/10.2298/PIF1203219G

Gašić K, Prokić A, Ivanović M, Kuzmanović N, Obradović A. Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina. 2012;27(3):219-229.
doi:10.2298/PIF1203219G .

Gašić, Katarina, Prokić, Anđelka, Ivanović, Milan, Kuzmanović, Nemanja, Obradović, Aleksa, "Differentiation of Pseudomonas syringae pathovars originating from stone fruits" in Pesticidi i fitomedicina, 27, no. 3 (2012):219-229,
https://doi.org/10.2298/PIF1203219G . .

TY  - JOUR
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Šević, Milan
AU  - Pulawska, J.
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/866
AB  - Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.
PB  - Springer
T2  - Journal of Plant Pathology
T1  - Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina
IS  - 1
VL  - 94
DO  - 10.4454/jpp.v94i1sup.020
ER  - 

@article{
author = "Prokić, Anđelka and Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Šević, Milan and Pulawska, J. and Obradović, Aleksa",
year = "2012",
abstract = "Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.",
publisher = "Springer",
journal = "Journal of Plant Pathology",
title = "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina",
number = "1",
volume = "94",
doi = "10.4454/jpp.v94i1sup.020"
}

Prokić, A., Gašić, K., Ivanović, M., Kuzmanović, N., Šević, M., Pulawska, J.,& Obradović, A.. (2012). Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina. in Journal of Plant Pathology
Springer., 94(1).
https://doi.org/10.4454/jpp.v94i1sup.020

Prokić A, Gašić K, Ivanović M, Kuzmanović N, Šević M, Pulawska J, Obradović A. Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina. in Journal of Plant Pathology. 2012;94(1).
doi:10.4454/jpp.v94i1sup.020 .

Prokić, Anđelka, Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Šević, Milan, Pulawska, J., Obradović, Aleksa, "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina" in Journal of Plant Pathology, 94, no. 1 (2012),
https://doi.org/10.4454/jpp.v94i1sup.020 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Kuzmanović, Nemanja
AU  - Ćalić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1077
AB  - U voćarstvu naše zemlje koštičave voćke zauzimaju značajno mesto i njihovo gajenje predstavlja profitabilnu delatnost. Međutim, rentabilnost voćarske proizvodnje mogu ugroziti različite biljne bolesti. Po značaju, raširenosti, ali i nemogućnosti uspešne kontrole, izdvajaju se oboljenja bakteriozne i fitoplazmozne prirode. Bakteriozno izumiranje, koje prouzrokuje Pseudomo nas syringae sa svoja dva patogena varijeteta syringae i morsprunorum, zadaje ozbiljne probleme voćarima u našoj zemlji. Značajan prob lem, takođe, predstavlja i Agrobacterium tumefaciens, prouzrokovač bakterioznog raka korena, koji nanosi štete u rasadničarskoj proizvodnji. U radu su pred stavljena i oboljenja koja nisu prisutna u našoj zemlji, a u svetu su opisana kao izuzetno destruktivna. To su bakteriozno izumiranje breskve, koje prouzrokuje P. s. pv. persicae i bakteriozna pegavost lišća koštičavih voćaka, čiji je prouzrokovač Xanthomonas arboricola pv. pruni. U Srbiji je prisutno i fitoplazmozno oboljenje Evropsko žutilo koštičavih voćaka na kajsiji i šljivi, koje za sada ne predstavlja ozbiljan problem. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja koštičavih voćaka, kako bi se donela pravilna i pravovremena dijagnoza od koje zavisi uspešnost mera zaštite.
PB  - Poljoprivredni fakultet, Novi Sad
T2  - Biljni lekar
T1  - Bakterioze i fitoplazmoze koštičavih voćaka
EP  - 338
IS  - 4-5
SP  - 323
VL  - 38
ER  - 

@article{
author = "Obradović, Aleksa and Kuzmanović, Nemanja and Ćalić, Anđelka and Gašić, Katarina and Ivanović, Milan",
year = "2010",
abstract = "U voćarstvu naše zemlje koštičave voćke zauzimaju značajno mesto i njihovo gajenje predstavlja profitabilnu delatnost. Međutim, rentabilnost voćarske proizvodnje mogu ugroziti različite biljne bolesti. Po značaju, raširenosti, ali i nemogućnosti uspešne kontrole, izdvajaju se oboljenja bakteriozne i fitoplazmozne prirode. Bakteriozno izumiranje, koje prouzrokuje Pseudomo nas syringae sa svoja dva patogena varijeteta syringae i morsprunorum, zadaje ozbiljne probleme voćarima u našoj zemlji. Značajan prob lem, takođe, predstavlja i Agrobacterium tumefaciens, prouzrokovač bakterioznog raka korena, koji nanosi štete u rasadničarskoj proizvodnji. U radu su pred stavljena i oboljenja koja nisu prisutna u našoj zemlji, a u svetu su opisana kao izuzetno destruktivna. To su bakteriozno izumiranje breskve, koje prouzrokuje P. s. pv. persicae i bakteriozna pegavost lišća koštičavih voćaka, čiji je prouzrokovač Xanthomonas arboricola pv. pruni. U Srbiji je prisutno i fitoplazmozno oboljenje Evropsko žutilo koštičavih voćaka na kajsiji i šljivi, koje za sada ne predstavlja ozbiljan problem. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja koštičavih voćaka, kako bi se donela pravilna i pravovremena dijagnoza od koje zavisi uspešnost mera zaštite.",
publisher = "Poljoprivredni fakultet, Novi Sad",
journal = "Biljni lekar",
title = "Bakterioze i fitoplazmoze koštičavih voćaka",
pages = "338-323",
number = "4-5",
volume = "38"
}

Obradović, A., Kuzmanović, N., Ćalić, A., Gašić, K.,& Ivanović, M.. (2010). Bakterioze i fitoplazmoze koštičavih voćaka. in Biljni lekar
Poljoprivredni fakultet, Novi Sad., 38(4-5), 323-338.

Obradović A, Kuzmanović N, Ćalić A, Gašić K, Ivanović M. Bakterioze i fitoplazmoze koštičavih voćaka. in Biljni lekar. 2010;38(4-5):323-338..

Obradović, Aleksa, Kuzmanović, Nemanja, Ćalić, Anđelka, Gašić, Katarina, Ivanović, Milan, "Bakterioze i fitoplazmoze koštičavih voćaka" in Biljni lekar, 38, no. 4-5 (2010):323-338.