TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Aćimović, Milica
AU  - Medic-Pap, Sladjana
AU  - Ivanović, Žarko
PY  - 2022-06
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/767
AB  - Hyssop (Hyssopus officinalis L.) is a perennial polymorphous plant species with essential oil
mainly accumulated in the flowers and leaves. It is grown in Serbia for the needs of
pharmaceutical companies and tea production, because of its quality and chemical
composition. During a routine quality control of hyssop seeds collected from Rumenka
(Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an
average of 22%. Infected seeds were covered with white mycelium followed with violet
pigmentation occurring under the seeds. The presence of Fusarium spp. was confirmed with
microscopic observation. Isolation was done aseptically by arranging infected seeds onto
surface of potato dextrose agar (PDA), and incubated at 25 °C with a 12-h photoperiod
(Mathur and Kongsdall, 2003). After seven days, 12 Fusarium spp. isolates were designated
as JBL 4003/1 - 4003/12. Pathogenicity test was performed in vitro using a modified agar
slant method in the test tube with PDA amended. After 10 days, fungal mycelia of tested
isolates caused seed rot and seedling decay, like naturally infected hyssop seeds. All isolates
were re-isolated and sub-cultured on Potato Dextrose Agar (PDA) and Carnation Leaf Agar
(CLA) using a hyphal tip transfer technique, fulfilling Koch's postulates. Isolate JBL 4003/1
was distinguished based on pathogenicity and cultural characteristics. It caused seed rot after
four days, on PDA colony was fast growing reaching 6-8 cm in diam. in five days, forming
abundant, whitish to peach aerial mycelium followed with beige to light brown pigmentation
in agar. Isolate formed relatively long and narrow macroconidia (24 to 54 × 3.2 to 4.5 μm)
with a tapered and elongated apical cell and prominent foot-shaped basal cell, with four to six
septate, with no microconidia. Chlamydospores were solitary and intercalary. Based cultural
and morphological characteristics indicated that the isolate belong to species Fusarium
equiseti Corda (Saccardo). To obtain a DNA sequence-based identification, total DNA was
extracted directly from the mycelium. Following DNA extraction, the translation elongation
factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2. The
amplified and purified DNA fragment of chosen isolate JBL4003/1 was sequenced in both
directions and deposited in the GeneBank under Accession Number MK061540.1. BLAST
analysis revealed that the Serbian isolate MK061540 showed the highest nucleotide identity
of 100% with F. equiseti isolates from United States (MG826890), Canada (KU587617),
Turkey (KT286761), and Serbia (JQ412101). Based on morphological and pathogenic
properties, as well as the sequence analysis, to our knowledge, this is the first case of F.
equiseti Corda (Saccardo) as the causal agent of Hyssopus officinalis (L.) seed rot in Serbia.
Considering the importance of H. Officinalis in pharmaceutical industries, knowledge of the
composition of populations of Fusarium species transmitted by hyssop.
PB  - Matica srpska
C3  - Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022
T1  - Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.
EP  - 67
SP  - 67
ER  - 

@conference{
author = "Ignjatov, Maja and Milošević, Dragana and Aćimović, Milica and Medic-Pap, Sladjana and Ivanović, Žarko",
year = "2022-06",
abstract = "Hyssop (Hyssopus officinalis L.) is a perennial polymorphous plant species with essential oil
mainly accumulated in the flowers and leaves. It is grown in Serbia for the needs of
pharmaceutical companies and tea production, because of its quality and chemical
composition. During a routine quality control of hyssop seeds collected from Rumenka
(Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an
average of 22%. Infected seeds were covered with white mycelium followed with violet
pigmentation occurring under the seeds. The presence of Fusarium spp. was confirmed with
microscopic observation. Isolation was done aseptically by arranging infected seeds onto
surface of potato dextrose agar (PDA), and incubated at 25 °C with a 12-h photoperiod
(Mathur and Kongsdall, 2003). After seven days, 12 Fusarium spp. isolates were designated
as JBL 4003/1 - 4003/12. Pathogenicity test was performed in vitro using a modified agar
slant method in the test tube with PDA amended. After 10 days, fungal mycelia of tested
isolates caused seed rot and seedling decay, like naturally infected hyssop seeds. All isolates
were re-isolated and sub-cultured on Potato Dextrose Agar (PDA) and Carnation Leaf Agar
(CLA) using a hyphal tip transfer technique, fulfilling Koch's postulates. Isolate JBL 4003/1
was distinguished based on pathogenicity and cultural characteristics. It caused seed rot after
four days, on PDA colony was fast growing reaching 6-8 cm in diam. in five days, forming
abundant, whitish to peach aerial mycelium followed with beige to light brown pigmentation
in agar. Isolate formed relatively long and narrow macroconidia (24 to 54 × 3.2 to 4.5 μm)
with a tapered and elongated apical cell and prominent foot-shaped basal cell, with four to six
septate, with no microconidia. Chlamydospores were solitary and intercalary. Based cultural
and morphological characteristics indicated that the isolate belong to species Fusarium
equiseti Corda (Saccardo). To obtain a DNA sequence-based identification, total DNA was
extracted directly from the mycelium. Following DNA extraction, the translation elongation
factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2. The
amplified and purified DNA fragment of chosen isolate JBL4003/1 was sequenced in both
directions and deposited in the GeneBank under Accession Number MK061540.1. BLAST
analysis revealed that the Serbian isolate MK061540 showed the highest nucleotide identity
of 100% with F. equiseti isolates from United States (MG826890), Canada (KU587617),
Turkey (KT286761), and Serbia (JQ412101). Based on morphological and pathogenic
properties, as well as the sequence analysis, to our knowledge, this is the first case of F.
equiseti Corda (Saccardo) as the causal agent of Hyssopus officinalis (L.) seed rot in Serbia.
Considering the importance of H. Officinalis in pharmaceutical industries, knowledge of the
composition of populations of Fusarium species transmitted by hyssop.",
publisher = "Matica srpska",
journal = "Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022",
title = "Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.",
pages = "67-67"
}

Ignjatov, M., Milošević, D., Aćimović, M., Medic-Pap, S.,& Ivanović, Ž.. (2022-06). Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022
Matica srpska., 67-67.

Ignjatov M, Milošević D, Aćimović M, Medic-Pap S, Ivanović Ž. Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022. 2022;:67-67..

Ignjatov, Maja, Milošević, Dragana, Aćimović, Milica, Medic-Pap, Sladjana, Ivanović, Žarko, "Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L." in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022 (2022-06):67-67.

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1144
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}

Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN

Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/685
AB  - Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Genetic Diversity of Pectobacterium spp. on Potato in Serbia
IS  - 9
VL  - 10
DO  - 10.3390/microorganisms10091840
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ivanović, Žarko and Ignjatov, Maja and Budakov, Dragana and Grahovac, Jovana and Grahovac, Mila",
year = "2022",
abstract = "Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Genetic Diversity of Pectobacterium spp. on Potato in Serbia",
number = "9",
volume = "10",
doi = "10.3390/microorganisms10091840"
}

Loc, M., Milošević, D., Ivanović, Ž., Ignjatov, M., Budakov, D., Grahovac, J.,& Grahovac, M.. (2022). Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms
MDPI., 10(9).
https://doi.org/10.3390/microorganisms10091840

Loc M, Milošević D, Ivanović Ž, Ignjatov M, Budakov D, Grahovac J, Grahovac M. Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms. 2022;10(9).
doi:10.3390/microorganisms10091840 .

Loc, Marta, Milošević, Dragana, Ivanović, Žarko, Ignjatov, Maja, Budakov, Dragana, Grahovac, Jovana, Grahovac, Mila, "Genetic Diversity of Pectobacterium spp. on Potato in Serbia" in Microorganisms, 10, no. 9 (2022),
https://doi.org/10.3390/microorganisms10091840 . .

TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Miklic, Vladimir
AU  - Dedic, Bosko
AU  - Tamindžić, Gordana
AU  - Bjelić, Dragana
AU  - Ivanović, Žarko
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/776
AB  - Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.
PB  - International Sunflower Association, Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad
T1  - Botrytis cinerea as causal agent of sunflower seed grey mould
SP  - 209
ER  - 

@conference{
author = "Ignjatov, Maja and Milošević, Dragana and Miklic, Vladimir and Dedic, Bosko and Tamindžić, Gordana and Bjelić, Dragana and Ivanović, Žarko",
year = "2022",
abstract = "Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.",
publisher = "International Sunflower Association, Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad",
title = "Botrytis cinerea as causal agent of sunflower seed grey mould",
pages = "209"
}

Ignjatov, M., Milošević, D., Miklic, V., Dedic, B., Tamindžić, G., Bjelić, D.,& Ivanović, Ž.. (2022). Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad
International Sunflower Association, Institute of Field and Vegetable Crops., 209.

Ignjatov M, Milošević D, Miklic V, Dedic B, Tamindžić G, Bjelić D, Ivanović Ž. Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad. 2022;:209..

Ignjatov, Maja, Milošević, Dragana, Miklic, Vladimir, Dedic, Bosko, Tamindžić, Gordana, Bjelić, Dragana, Ivanović, Žarko, "Botrytis cinerea as causal agent of sunflower seed grey mould" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad (2022):209.

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Milošević, D., Bulajić, A.,& Krstić, B.. (2013). First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia. in Plant Disease
American Phytopathological Society., 97(7), 1004.
https://doi.org/10.1094/PDIS-01-13-0089-PDN

Milojević K, Stanković I, Vučurović A, Ristić D, Milošević D, Bulajić A, Krstić B. First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia. in Plant Disease. 2013;97(7):1004.
doi:10.1094/PDIS-01-13-0089-PDN .

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Milošević, Dragana, Bulajić, Aleksandra, Krstić, Branka, "First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia" in Plant Disease, 97, no. 7 (2013):1004,
https://doi.org/10.1094/PDIS-01-13-0089-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1135
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 

@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}

Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769

Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .

Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .