TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1144
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}

Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN

Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/685
AB  - Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Genetic Diversity of Pectobacterium spp. on Potato in Serbia
IS  - 9
VL  - 10
DO  - 10.3390/microorganisms10091840
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ivanović, Žarko and Ignjatov, Maja and Budakov, Dragana and Grahovac, Jovana and Grahovac, Mila",
year = "2022",
abstract = "Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Genetic Diversity of Pectobacterium spp. on Potato in Serbia",
number = "9",
volume = "10",
doi = "10.3390/microorganisms10091840"
}

Loc, M., Milošević, D., Ivanović, Ž., Ignjatov, M., Budakov, D., Grahovac, J.,& Grahovac, M.. (2022). Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms
MDPI., 10(9).
https://doi.org/10.3390/microorganisms10091840

Loc M, Milošević D, Ivanović Ž, Ignjatov M, Budakov D, Grahovac J, Grahovac M. Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms. 2022;10(9).
doi:10.3390/microorganisms10091840 .

Loc, Marta, Milošević, Dragana, Ivanović, Žarko, Ignjatov, Maja, Budakov, Dragana, Grahovac, Jovana, Grahovac, Mila, "Genetic Diversity of Pectobacterium spp. on Potato in Serbia" in Microorganisms, 10, no. 9 (2022),
https://doi.org/10.3390/microorganisms10091840 . .