TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .