TY  - JOUR
AU  - Đurović, Sanja
AU  - Dragičević, Vesna
AU  - Waisi, Hadi
AU  - Pagnacco, Maja
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
AU  - Nikolić, Bogdan
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/591
AB  - Abstract: Polyphenols present in different plant cell organelles increase the resistance of plants to various types of environmental
stresses. We investigated the possibility of increasing the content of bioactive compounds in the seed of yellow
soybean variety Laura. The soybean was treated during vegetation with five products based on plant extracts, on the
assumption of enrichment of plants with various nutrients. Soybean flour extracts were screened spectrophotometrically
for total phenolic content and antioxidant activity. The antioxidant activity was evaluated using three methods. The content
of phenolic acids was determined by HPLC, and the raw protein content was estimated by the Kjeldahl method. Depending
on the treatment, variations in the quantity of individual phenolic acids with up to 90% higher concentration as compared
to the control were observed. Controlled usage of certain plant extracts can increase the concentration of the target group of
bioactive compounds in the samples. The synergistic effect of proteins and phenolic compounds on the antioxidant activity
of extracts was detected. The results of this study are not only important from the aspect of plant resistance to various types
of stress, but also when considering soybean as a functional food.
PB  - Belgrade : Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products
EP  - 434
IS  - 3
SP  - 425
VL  - 73
DO  - 10.2298/ABS190123024D
ER  - 

@article{
author = "Đurović, Sanja and Dragičević, Vesna and Waisi, Hadi and Pagnacco, Maja and Luković, Nevena and Knežević-Jugović, Zorica and Nikolić, Bogdan",
year = "2019",
abstract = "Abstract: Polyphenols present in different plant cell organelles increase the resistance of plants to various types of environmental
stresses. We investigated the possibility of increasing the content of bioactive compounds in the seed of yellow
soybean variety Laura. The soybean was treated during vegetation with five products based on plant extracts, on the
assumption of enrichment of plants with various nutrients. Soybean flour extracts were screened spectrophotometrically
for total phenolic content and antioxidant activity. The antioxidant activity was evaluated using three methods. The content
of phenolic acids was determined by HPLC, and the raw protein content was estimated by the Kjeldahl method. Depending
on the treatment, variations in the quantity of individual phenolic acids with up to 90% higher concentration as compared
to the control were observed. Controlled usage of certain plant extracts can increase the concentration of the target group of
bioactive compounds in the samples. The synergistic effect of proteins and phenolic compounds on the antioxidant activity
of extracts was detected. The results of this study are not only important from the aspect of plant resistance to various types
of stress, but also when considering soybean as a functional food.",
publisher = "Belgrade : Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products",
pages = "434-425",
number = "3",
volume = "73",
doi = "10.2298/ABS190123024D"
}

Đurović, S., Dragičević, V., Waisi, H., Pagnacco, M., Luković, N., Knežević-Jugović, Z.,& Nikolić, B.. (2019). Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products. in Archives of Biological Sciences
Belgrade : Serbian Biological Society., 73(3), 425-434.
https://doi.org/10.2298/ABS190123024D

Đurović S, Dragičević V, Waisi H, Pagnacco M, Luković N, Knežević-Jugović Z, Nikolić B. Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products. in Archives of Biological Sciences. 2019;73(3):425-434.
doi:10.2298/ABS190123024D .

Đurović, Sanja, Dragičević, Vesna, Waisi, Hadi, Pagnacco, Maja, Luković, Nevena, Knežević-Jugović, Zorica, Nikolić, Bogdan, "Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products" in Archives of Biological Sciences, 73, no. 3 (2019):425-434,
https://doi.org/10.2298/ABS190123024D . .

TY  - JOUR
AU  - Đurović, Sanja
AU  - Nikolić, Bogdan
AU  - Luković, Nevena
AU  - Jovanović, Jelena
AU  - Stefanović, Andrea
AU  - Sekuljica, Natasa
AU  - Mijin, Dušan
AU  - Knezevic-Jugović, Zorica
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/509
AB  - The aim of the study was to investigate and compare several extraction protocols like 1) high-power ultrasound probe assisted solvent extraction; 2) microwave assisted solvent extraction; 3) direct acid hydrolysis; 4) direct alkali hydrolysis, and 5) two step extraction consisting of ultrasound or microwave assisted solvent extraction followed by alkaline and acid hydrolysis in terms of efficiency of the extraction of phenolic acids from the yellow soybean seed variety Laura. These extracts were screened for their total phenol content (TPC), and for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) assay, as well as on content of some individual phenolic acids. It appeared that the acetone containing mixtures gave much higher TPC than methanol containing ones, but the presence of acid in the methanol solvent significantly improved the extraction of phenolic compounds. To further improve the extraction, an ultrasound lab-scale probe at 20 kHz was used, with 15 and 30% of the maximum amplitude, and the extraction time was varied from 2 to 15 min. Microwave assisted extraction was performed varying the temperature from 55 to 85 degrees C, microwave power from 25 to 100 W and extraction time from 2 to 10 min. Changes in the content of six phenolic acids were examined: gallic, trans cinnamic, chlorogenic, caffeic, p-coumaric and ferulic acid. The separation and quantification of phenolic acids was accomplished by high-performance liquid chromatography-diode array detection (DAD) procedure. The results suggested that microwaves contributed to more efficient extraction of phenolic acids from the seed of yellow soybean. The amount of phenolic acids varied from 65.52 mu g/g of dry matter (d.m.) for caffeic acid, to 581.84 mu g/g d.m. for p-coumaric acid. Both, ultrasound and microwaves contributed to more efficient extraction of total phenol compounds and enhanced antioxidant activity of soybean seed extracts. TPC varied from 12.48 to 18.77 mg GAE/g d.m. and antioxidant activity varied from 244.58 to 345.21 mu mol TROLOX eq/g d.m.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - The impact of high-power ultrasound and microwave on the phenolic acid profile and antioxidant activity of the extract from yellow soybean seeds
EP  - 231
SP  - 223
VL  - 122
DO  - 10.1016/j.indcrop.2018.05.078
ER  - 

@article{
author = "Đurović, Sanja and Nikolić, Bogdan and Luković, Nevena and Jovanović, Jelena and Stefanović, Andrea and Sekuljica, Natasa and Mijin, Dušan and Knezevic-Jugović, Zorica",
year = "2018",
abstract = "The aim of the study was to investigate and compare several extraction protocols like 1) high-power ultrasound probe assisted solvent extraction; 2) microwave assisted solvent extraction; 3) direct acid hydrolysis; 4) direct alkali hydrolysis, and 5) two step extraction consisting of ultrasound or microwave assisted solvent extraction followed by alkaline and acid hydrolysis in terms of efficiency of the extraction of phenolic acids from the yellow soybean seed variety Laura. These extracts were screened for their total phenol content (TPC), and for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) assay, as well as on content of some individual phenolic acids. It appeared that the acetone containing mixtures gave much higher TPC than methanol containing ones, but the presence of acid in the methanol solvent significantly improved the extraction of phenolic compounds. To further improve the extraction, an ultrasound lab-scale probe at 20 kHz was used, with 15 and 30% of the maximum amplitude, and the extraction time was varied from 2 to 15 min. Microwave assisted extraction was performed varying the temperature from 55 to 85 degrees C, microwave power from 25 to 100 W and extraction time from 2 to 10 min. Changes in the content of six phenolic acids were examined: gallic, trans cinnamic, chlorogenic, caffeic, p-coumaric and ferulic acid. The separation and quantification of phenolic acids was accomplished by high-performance liquid chromatography-diode array detection (DAD) procedure. The results suggested that microwaves contributed to more efficient extraction of phenolic acids from the seed of yellow soybean. The amount of phenolic acids varied from 65.52 mu g/g of dry matter (d.m.) for caffeic acid, to 581.84 mu g/g d.m. for p-coumaric acid. Both, ultrasound and microwaves contributed to more efficient extraction of total phenol compounds and enhanced antioxidant activity of soybean seed extracts. TPC varied from 12.48 to 18.77 mg GAE/g d.m. and antioxidant activity varied from 244.58 to 345.21 mu mol TROLOX eq/g d.m.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "The impact of high-power ultrasound and microwave on the phenolic acid profile and antioxidant activity of the extract from yellow soybean seeds",
pages = "231-223",
volume = "122",
doi = "10.1016/j.indcrop.2018.05.078"
}

Đurović, S., Nikolić, B., Luković, N., Jovanović, J., Stefanović, A., Sekuljica, N., Mijin, D.,& Knezevic-Jugović, Z.. (2018). The impact of high-power ultrasound and microwave on the phenolic acid profile and antioxidant activity of the extract from yellow soybean seeds. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 122, 223-231.
https://doi.org/10.1016/j.indcrop.2018.05.078

Đurović S, Nikolić B, Luković N, Jovanović J, Stefanović A, Sekuljica N, Mijin D, Knezevic-Jugović Z. The impact of high-power ultrasound and microwave on the phenolic acid profile and antioxidant activity of the extract from yellow soybean seeds. in Industrial Crops and Products. 2018;122:223-231.
doi:10.1016/j.indcrop.2018.05.078 .

Đurović, Sanja, Nikolić, Bogdan, Luković, Nevena, Jovanović, Jelena, Stefanović, Andrea, Sekuljica, Natasa, Mijin, Dušan, Knezevic-Jugović, Zorica, "The impact of high-power ultrasound and microwave on the phenolic acid profile and antioxidant activity of the extract from yellow soybean seeds" in Industrial Crops and Products, 122 (2018):223-231,
https://doi.org/10.1016/j.indcrop.2018.05.078 . .