TY  - JOUR
AU  - Blagojević, Jovana
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Starović, Mira
AU  - Ristić, Danijela
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1180
AB  - Identifying the precise pathogens responsible for specific plant diseases is imperative for implementing targeted and efficient interventions and mitigating their spread. Dieback and shoot blight significantly diminish the lifespan and productivity of blueberries, yet the causative agents remain largely unidentified. To determine the identity and prevalence of the causal agents of branch dieback and shoot blight, we conducted multi-year and multi-site sampling of diseased highbush blueberries (Vaccinium corymbosum cv. Duke) in Serbia. Sixty-nine monosporic isolates were collected and characterized based on morphological, physiological features and multi-locus phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (TUB2) and translation elongation factor 1-alpha (TEF1) sequence data. Five species were identified as causal agents: Diaporthe eres (36 isolates), D. foeniculina (3 isolates), Neopestalotiopsis vaccinii (9 isolates), N. rosae (6 isolates) and Neofusicoccum parvum (15 isolates). The results of the pathogenicity tests performed with the 23 representative isolates confirmed the role of these species as primary pathogens in causing dieback and shoot blight of blueberry, with N. parvum being the most aggressive and D. eres the least. Our study underscores the diversity of genera and species of ascomycetes capable of causing blueberry dieback and shoot blight. Furthermore, our findings indicate that the agents responsible for the disease in Serbia differ from those identified in other regions worldwide.
PB  - The American Phytopathological Society
T2  - Phytopathology
T1  - Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia
DO  - https://doi.org/10.1094/PHYTO-04-23-0133-R
ER  - 

@article{
author = "Blagojević, Jovana and Aleksic, Goran and Vučurović, Ivan and Starović, Mira and Ristić, Danijela",
year = "2023",
abstract = "Identifying the precise pathogens responsible for specific plant diseases is imperative for implementing targeted and efficient interventions and mitigating their spread. Dieback and shoot blight significantly diminish the lifespan and productivity of blueberries, yet the causative agents remain largely unidentified. To determine the identity and prevalence of the causal agents of branch dieback and shoot blight, we conducted multi-year and multi-site sampling of diseased highbush blueberries (Vaccinium corymbosum cv. Duke) in Serbia. Sixty-nine monosporic isolates were collected and characterized based on morphological, physiological features and multi-locus phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (TUB2) and translation elongation factor 1-alpha (TEF1) sequence data. Five species were identified as causal agents: Diaporthe eres (36 isolates), D. foeniculina (3 isolates), Neopestalotiopsis vaccinii (9 isolates), N. rosae (6 isolates) and Neofusicoccum parvum (15 isolates). The results of the pathogenicity tests performed with the 23 representative isolates confirmed the role of these species as primary pathogens in causing dieback and shoot blight of blueberry, with N. parvum being the most aggressive and D. eres the least. Our study underscores the diversity of genera and species of ascomycetes capable of causing blueberry dieback and shoot blight. Furthermore, our findings indicate that the agents responsible for the disease in Serbia differ from those identified in other regions worldwide.",
publisher = "The American Phytopathological Society",
journal = "Phytopathology",
title = "Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia",
doi = "https://doi.org/10.1094/PHYTO-04-23-0133-R"
}

Blagojević, J., Aleksic, G., Vučurović, I., Starović, M.,& Ristić, D.. (2023). Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia. in Phytopathology
The American Phytopathological Society..
https://doi.org/https://doi.org/10.1094/PHYTO-04-23-0133-R

Blagojević J, Aleksic G, Vučurović I, Starović M, Ristić D. Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia. in Phytopathology. 2023;.
doi:https://doi.org/10.1094/PHYTO-04-23-0133-R .

Blagojević, Jovana, Aleksic, Goran, Vučurović, Ivan, Starović, Mira, Ristić, Danijela, "Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia" in Phytopathology (2023),
https://doi.org/https://doi.org/10.1094/PHYTO-04-23-0133-R . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/747
AB  - Alternaria solani is a fungal pathogen that causes early blight, an economically important disease of potatoes worldwide. The application of fungicides differing in mode of action are the most important control measure for early blight management. However, frequent use of fungicides may cause fungal pathogen populations to be selected for mutations conferring fungicide resistance, resulting in significant losses of fungicide efficacy. Repeated use of QoI fungicides may generate a selection pressure on A. solani populations that promotes resistant genotypes, this quality in question being because of amino acid substitutions in the cytochrome b gene. During a four-year survey, isolates of A. solani were collected from the primary potato production regions in Serbia. The cytochrome b gene region of each isolate was evaluated for the presence of the F129L substitution known to historically confer QoI resistance. Sequence analysis revealed two genotypes similar to those reported elsewhere in the world with a diverse geographic distribution in Serbia. Genotype I was dominant in the south of Serbia, genotype II was prevalent in north Serbia, and in central Serbia both genotypes were represented equally. The F129L mutation was detected in both genotypes, with a progressive shift from the wild type to mutated A. solani across years. A. solani isolates containing the F129L mutation were collected from the northern and the central Serbia in 2016 without any mutated isolates in the southern Serbia. In 2019, in all surveyed regions, a predominance of isolates with the F129L mutation was observed. The presence of two cytochrome b genotypes among A. solani isolates and significant presence of F129L mutations indicates a change in the genetic structure of this pathogens populations in potato fields nationwide, which could have a significant impact on control of early blight disease.
PB  - Association of Applied Biologists
T2  - Annals of Applied Biology
T1  - Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia
EP  - 126
IS  - 1
SP  - 117
VL  - 18
DO  - 10.1111/aab.12763
DO  - 1744-7348
ER  - 

@article{
author = "Ivanović, Žarko and Blagojević, Jovana",
year = "2022",
abstract = "Alternaria solani is a fungal pathogen that causes early blight, an economically important disease of potatoes worldwide. The application of fungicides differing in mode of action are the most important control measure for early blight management. However, frequent use of fungicides may cause fungal pathogen populations to be selected for mutations conferring fungicide resistance, resulting in significant losses of fungicide efficacy. Repeated use of QoI fungicides may generate a selection pressure on A. solani populations that promotes resistant genotypes, this quality in question being because of amino acid substitutions in the cytochrome b gene. During a four-year survey, isolates of A. solani were collected from the primary potato production regions in Serbia. The cytochrome b gene region of each isolate was evaluated for the presence of the F129L substitution known to historically confer QoI resistance. Sequence analysis revealed two genotypes similar to those reported elsewhere in the world with a diverse geographic distribution in Serbia. Genotype I was dominant in the south of Serbia, genotype II was prevalent in north Serbia, and in central Serbia both genotypes were represented equally. The F129L mutation was detected in both genotypes, with a progressive shift from the wild type to mutated A. solani across years. A. solani isolates containing the F129L mutation were collected from the northern and the central Serbia in 2016 without any mutated isolates in the southern Serbia. In 2019, in all surveyed regions, a predominance of isolates with the F129L mutation was observed. The presence of two cytochrome b genotypes among A. solani isolates and significant presence of F129L mutations indicates a change in the genetic structure of this pathogens populations in potato fields nationwide, which could have a significant impact on control of early blight disease.",
publisher = "Association of Applied Biologists",
journal = "Annals of Applied Biology",
title = "Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia",
pages = "126-117",
number = "1",
volume = "18",
doi = "10.1111/aab.12763, 1744-7348"
}

Ivanović, Ž.,& Blagojević, J.. (2022). Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia. in Annals of Applied Biology
Association of Applied Biologists., 18(1), 117-126.
https://doi.org/10.1111/aab.12763

Ivanović Ž, Blagojević J. Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia. in Annals of Applied Biology. 2022;18(1):117-126.
doi:10.1111/aab.12763 .

Ivanović, Žarko, Blagojević, Jovana, "Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia" in Annals of Applied Biology, 18, no. 1 (2022):117-126,
https://doi.org/10.1111/aab.12763 . .

Blagojević, J., Ivanović, Ž., Popović Milovanović, T.,& Ignjatov, M.. (2017). First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease
American Phytopathological Society., 101(2), 384-385.
https://doi.org/10.1094/PDIS-08-16-1106-PDN

Blagojević J, Ivanović Ž, Popović Milovanović T, Ignjatov M. First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease. 2017;101(2):384-385.
doi:10.1094/PDIS-08-16-1106-PDN .

Blagojević, Jovana, Ivanović, Žarko, Popović Milovanović, Tatjana, Ignjatov, Maja, "First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia" in Plant Disease, 101, no. 2 (2017):384-385,
https://doi.org/10.1094/PDIS-08-16-1106-PDN . .