TY  - JOUR
AU  - Ivanović, Žarko
AU  - Marisavljević, Dragana
AU  - Marinković, Radovan
AU  - Mitrović, Petar
AU  - Blagojević, Jovana
AU  - Nikolić, Ivan
AU  - Pavlovic, Danijela
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/729
AB  - In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum span- ning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.
PB  - Korean Society of Plant Pathology
T2  - The Plant Pathology Journal
T1  - Genetic Diversity of Orobanche cumana Populations in Serbia
EP  - 520
IS  - 6
SP  - 512
VL  - 37
DO  - 10.5423/PPJ.OA.04.2021.0066
DO  - 1598-2254
ER  - 

@article{
author = "Ivanović, Žarko and Marisavljević, Dragana and Marinković, Radovan and Mitrović, Petar and Blagojević, Jovana and Nikolić, Ivan and Pavlovic, Danijela",
year = "2021",
abstract = "In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum span- ning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.",
publisher = "Korean Society of Plant Pathology",
journal = "The Plant Pathology Journal",
title = "Genetic Diversity of Orobanche cumana Populations in Serbia",
pages = "520-512",
number = "6",
volume = "37",
doi = "10.5423/PPJ.OA.04.2021.0066, 1598-2254"
}

Ivanović, Ž., Marisavljević, D., Marinković, R., Mitrović, P., Blagojević, J., Nikolić, I.,& Pavlovic, D.. (2021). Genetic Diversity of Orobanche cumana Populations in Serbia. in The Plant Pathology Journal
Korean Society of Plant Pathology., 37(6), 512-520.
https://doi.org/10.5423/PPJ.OA.04.2021.0066

Ivanović Ž, Marisavljević D, Marinković R, Mitrović P, Blagojević J, Nikolić I, Pavlovic D. Genetic Diversity of Orobanche cumana Populations in Serbia. in The Plant Pathology Journal. 2021;37(6):512-520.
doi:10.5423/PPJ.OA.04.2021.0066 .

Ivanović, Žarko, Marisavljević, Dragana, Marinković, Radovan, Mitrović, Petar, Blagojević, Jovana, Nikolić, Ivan, Pavlovic, Danijela, "Genetic Diversity of Orobanche cumana Populations in Serbia" in The Plant Pathology Journal, 37, no. 6 (2021):512-520,
https://doi.org/10.5423/PPJ.OA.04.2021.0066 . .