TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Aćimović, Milica
AU  - Medic-Pap, Sladjana
AU  - Ivanović, Žarko
PY  - 2022-06
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/767
AB  - Hyssop (Hyssopus officinalis L.) is a perennial polymorphous plant species with essential oil
mainly accumulated in the flowers and leaves. It is grown in Serbia for the needs of
pharmaceutical companies and tea production, because of its quality and chemical
composition. During a routine quality control of hyssop seeds collected from Rumenka
(Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an
average of 22%. Infected seeds were covered with white mycelium followed with violet
pigmentation occurring under the seeds. The presence of Fusarium spp. was confirmed with
microscopic observation. Isolation was done aseptically by arranging infected seeds onto
surface of potato dextrose agar (PDA), and incubated at 25 °C with a 12-h photoperiod
(Mathur and Kongsdall, 2003). After seven days, 12 Fusarium spp. isolates were designated
as JBL 4003/1 - 4003/12. Pathogenicity test was performed in vitro using a modified agar
slant method in the test tube with PDA amended. After 10 days, fungal mycelia of tested
isolates caused seed rot and seedling decay, like naturally infected hyssop seeds. All isolates
were re-isolated and sub-cultured on Potato Dextrose Agar (PDA) and Carnation Leaf Agar
(CLA) using a hyphal tip transfer technique, fulfilling Koch's postulates. Isolate JBL 4003/1
was distinguished based on pathogenicity and cultural characteristics. It caused seed rot after
four days, on PDA colony was fast growing reaching 6-8 cm in diam. in five days, forming
abundant, whitish to peach aerial mycelium followed with beige to light brown pigmentation
in agar. Isolate formed relatively long and narrow macroconidia (24 to 54 × 3.2 to 4.5 μm)
with a tapered and elongated apical cell and prominent foot-shaped basal cell, with four to six
septate, with no microconidia. Chlamydospores were solitary and intercalary. Based cultural
and morphological characteristics indicated that the isolate belong to species Fusarium
equiseti Corda (Saccardo). To obtain a DNA sequence-based identification, total DNA was
extracted directly from the mycelium. Following DNA extraction, the translation elongation
factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2. The
amplified and purified DNA fragment of chosen isolate JBL4003/1 was sequenced in both
directions and deposited in the GeneBank under Accession Number MK061540.1. BLAST
analysis revealed that the Serbian isolate MK061540 showed the highest nucleotide identity
of 100% with F. equiseti isolates from United States (MG826890), Canada (KU587617),
Turkey (KT286761), and Serbia (JQ412101). Based on morphological and pathogenic
properties, as well as the sequence analysis, to our knowledge, this is the first case of F.
equiseti Corda (Saccardo) as the causal agent of Hyssopus officinalis (L.) seed rot in Serbia.
Considering the importance of H. Officinalis in pharmaceutical industries, knowledge of the
composition of populations of Fusarium species transmitted by hyssop.
PB  - Matica srpska
C3  - Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022
T1  - Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.
EP  - 67
SP  - 67
ER  - 

@conference{
author = "Ignjatov, Maja and Milošević, Dragana and Aćimović, Milica and Medic-Pap, Sladjana and Ivanović, Žarko",
year = "2022-06",
abstract = "Hyssop (Hyssopus officinalis L.) is a perennial polymorphous plant species with essential oil
mainly accumulated in the flowers and leaves. It is grown in Serbia for the needs of
pharmaceutical companies and tea production, because of its quality and chemical
composition. During a routine quality control of hyssop seeds collected from Rumenka
(Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an
average of 22%. Infected seeds were covered with white mycelium followed with violet
pigmentation occurring under the seeds. The presence of Fusarium spp. was confirmed with
microscopic observation. Isolation was done aseptically by arranging infected seeds onto
surface of potato dextrose agar (PDA), and incubated at 25 °C with a 12-h photoperiod
(Mathur and Kongsdall, 2003). After seven days, 12 Fusarium spp. isolates were designated
as JBL 4003/1 - 4003/12. Pathogenicity test was performed in vitro using a modified agar
slant method in the test tube with PDA amended. After 10 days, fungal mycelia of tested
isolates caused seed rot and seedling decay, like naturally infected hyssop seeds. All isolates
were re-isolated and sub-cultured on Potato Dextrose Agar (PDA) and Carnation Leaf Agar
(CLA) using a hyphal tip transfer technique, fulfilling Koch's postulates. Isolate JBL 4003/1
was distinguished based on pathogenicity and cultural characteristics. It caused seed rot after
four days, on PDA colony was fast growing reaching 6-8 cm in diam. in five days, forming
abundant, whitish to peach aerial mycelium followed with beige to light brown pigmentation
in agar. Isolate formed relatively long and narrow macroconidia (24 to 54 × 3.2 to 4.5 μm)
with a tapered and elongated apical cell and prominent foot-shaped basal cell, with four to six
septate, with no microconidia. Chlamydospores were solitary and intercalary. Based cultural
and morphological characteristics indicated that the isolate belong to species Fusarium
equiseti Corda (Saccardo). To obtain a DNA sequence-based identification, total DNA was
extracted directly from the mycelium. Following DNA extraction, the translation elongation
factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2. The
amplified and purified DNA fragment of chosen isolate JBL4003/1 was sequenced in both
directions and deposited in the GeneBank under Accession Number MK061540.1. BLAST
analysis revealed that the Serbian isolate MK061540 showed the highest nucleotide identity
of 100% with F. equiseti isolates from United States (MG826890), Canada (KU587617),
Turkey (KT286761), and Serbia (JQ412101). Based on morphological and pathogenic
properties, as well as the sequence analysis, to our knowledge, this is the first case of F.
equiseti Corda (Saccardo) as the causal agent of Hyssopus officinalis (L.) seed rot in Serbia.
Considering the importance of H. Officinalis in pharmaceutical industries, knowledge of the
composition of populations of Fusarium species transmitted by hyssop.",
publisher = "Matica srpska",
journal = "Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022",
title = "Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.",
pages = "67-67"
}

Ignjatov, M., Milošević, D., Aćimović, M., Medic-Pap, S.,& Ivanović, Ž.. (2022-06). Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022
Matica srpska., 67-67.

Ignjatov M, Milošević D, Aćimović M, Medic-Pap S, Ivanović Ž. Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022. 2022;:67-67..

Ignjatov, Maja, Milošević, Dragana, Aćimović, Milica, Medic-Pap, Sladjana, Ivanović, Žarko, "Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L." in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022 (2022-06):67-67.

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1144
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}

Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN

Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .

TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Miklic, Vladimir
AU  - Dedic, Bosko
AU  - Tamindžić, Gordana
AU  - Bjelić, Dragana
AU  - Ivanović, Žarko
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/776
AB  - Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.
PB  - International Sunflower Association, Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad
T1  - Botrytis cinerea as causal agent of sunflower seed grey mould
SP  - 209
ER  - 

@conference{
author = "Ignjatov, Maja and Milošević, Dragana and Miklic, Vladimir and Dedic, Bosko and Tamindžić, Gordana and Bjelić, Dragana and Ivanović, Žarko",
year = "2022",
abstract = "Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.",
publisher = "International Sunflower Association, Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad",
title = "Botrytis cinerea as causal agent of sunflower seed grey mould",
pages = "209"
}

Ignjatov, M., Milošević, D., Miklic, V., Dedic, B., Tamindžić, G., Bjelić, D.,& Ivanović, Ž.. (2022). Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad
International Sunflower Association, Institute of Field and Vegetable Crops., 209.

Ignjatov M, Milošević D, Miklic V, Dedic B, Tamindžić G, Bjelić D, Ivanović Ž. Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad. 2022;:209..

Ignjatov, Maja, Milošević, Dragana, Miklic, Vladimir, Dedic, Bosko, Tamindžić, Gordana, Bjelić, Dragana, Ivanović, Žarko, "Botrytis cinerea as causal agent of sunflower seed grey mould" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad (2022):209.

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/747
AB  - Alternaria solani is a fungal pathogen that causes early blight, an economically important disease of potatoes worldwide. The application of fungicides differing in mode of action are the most important control measure for early blight management. However, frequent use of fungicides may cause fungal pathogen populations to be selected for mutations conferring fungicide resistance, resulting in significant losses of fungicide efficacy. Repeated use of QoI fungicides may generate a selection pressure on A. solani populations that promotes resistant genotypes, this quality in question being because of amino acid substitutions in the cytochrome b gene. During a four-year survey, isolates of A. solani were collected from the primary potato production regions in Serbia. The cytochrome b gene region of each isolate was evaluated for the presence of the F129L substitution known to historically confer QoI resistance. Sequence analysis revealed two genotypes similar to those reported elsewhere in the world with a diverse geographic distribution in Serbia. Genotype I was dominant in the south of Serbia, genotype II was prevalent in north Serbia, and in central Serbia both genotypes were represented equally. The F129L mutation was detected in both genotypes, with a progressive shift from the wild type to mutated A. solani across years. A. solani isolates containing the F129L mutation were collected from the northern and the central Serbia in 2016 without any mutated isolates in the southern Serbia. In 2019, in all surveyed regions, a predominance of isolates with the F129L mutation was observed. The presence of two cytochrome b genotypes among A. solani isolates and significant presence of F129L mutations indicates a change in the genetic structure of this pathogens populations in potato fields nationwide, which could have a significant impact on control of early blight disease.
PB  - Association of Applied Biologists
T2  - Annals of Applied Biology
T1  - Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia
EP  - 126
IS  - 1
SP  - 117
VL  - 18
DO  - 10.1111/aab.12763
DO  - 1744-7348
ER  - 

@article{
author = "Ivanović, Žarko and Blagojević, Jovana",
year = "2022",
abstract = "Alternaria solani is a fungal pathogen that causes early blight, an economically important disease of potatoes worldwide. The application of fungicides differing in mode of action are the most important control measure for early blight management. However, frequent use of fungicides may cause fungal pathogen populations to be selected for mutations conferring fungicide resistance, resulting in significant losses of fungicide efficacy. Repeated use of QoI fungicides may generate a selection pressure on A. solani populations that promotes resistant genotypes, this quality in question being because of amino acid substitutions in the cytochrome b gene. During a four-year survey, isolates of A. solani were collected from the primary potato production regions in Serbia. The cytochrome b gene region of each isolate was evaluated for the presence of the F129L substitution known to historically confer QoI resistance. Sequence analysis revealed two genotypes similar to those reported elsewhere in the world with a diverse geographic distribution in Serbia. Genotype I was dominant in the south of Serbia, genotype II was prevalent in north Serbia, and in central Serbia both genotypes were represented equally. The F129L mutation was detected in both genotypes, with a progressive shift from the wild type to mutated A. solani across years. A. solani isolates containing the F129L mutation were collected from the northern and the central Serbia in 2016 without any mutated isolates in the southern Serbia. In 2019, in all surveyed regions, a predominance of isolates with the F129L mutation was observed. The presence of two cytochrome b genotypes among A. solani isolates and significant presence of F129L mutations indicates a change in the genetic structure of this pathogens populations in potato fields nationwide, which could have a significant impact on control of early blight disease.",
publisher = "Association of Applied Biologists",
journal = "Annals of Applied Biology",
title = "Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia",
pages = "126-117",
number = "1",
volume = "18",
doi = "10.1111/aab.12763, 1744-7348"
}

Ivanović, Ž.,& Blagojević, J.. (2022). Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia. in Annals of Applied Biology
Association of Applied Biologists., 18(1), 117-126.
https://doi.org/10.1111/aab.12763

Ivanović Ž, Blagojević J. Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia. in Annals of Applied Biology. 2022;18(1):117-126.
doi:10.1111/aab.12763 .

Ivanović, Žarko, Blagojević, Jovana, "Distribution of the F129L mutation conferring resistance to strobilurins in Alternaria solani populations in Serbia" in Annals of Applied Biology, 18, no. 1 (2022):117-126,
https://doi.org/10.1111/aab.12763 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Jovanović, Gordana
AU  - Ivanović, Borko
AU  - Žeželj, Danica
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/746
AB  - Early blight is an economically important disease of potato worldwide. Understanding which fungal pathogens are the causal agents of early blight and their distribution on the same host is essential to finding the best strategy for the control of this disease. Previous studies have shown that Alternaria solani is the main early blight pathogen parasitizing potato. Here, we analyzed genetic and phenotypic diversity in isolates of Alternaria spp. covering all potato production areas in Serbia. We showed that the four species of Alternaria were found in areas with different distributions of the species. The occurrence of Alternaria spp. was studied by analyzing isolates from symptomatic potato leaves during multiyear sampling. In addition to Alternaria solani, we detected three more large-spored species identified as A. linariae (syn. A. tomatophila), A. protenta, and A. grandis that were involved in early blight disease on naturally infected potato leaves in Serbia. Differentiation of species was supported by phylogeny obtained from the DNA sequences of the GAPDH, calmodulin and Rpb2 genes. Our findings present a new perspective into the population structure of large-spored Alternaria species associated with early blight disease. Within the groups of large-spored Alternaria present in Serbia, evidence of A. protenta at high frequency reveals new insight into the contribution of Alternaria species in early blight disease. This work opens new perspectives for early blight management, while the distribution of different species on the same host suggests that the etiology of disease could depend on crop organization and the presence of other Alternaria hosts in close proximity to potato plants.
PB  - Frontiers Media
T2  - Frontiers in Microbiology
T1  - New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia
IS  - 856898
VL  - 13
DO  - 10.3389/fmicb.2022.856898
ER  - 

@article{
author = "Ivanović, Žarko and Blagojević, Jovana and Jovanović, Gordana and Ivanović, Borko and Žeželj, Danica",
year = "2022",
abstract = "Early blight is an economically important disease of potato worldwide. Understanding which fungal pathogens are the causal agents of early blight and their distribution on the same host is essential to finding the best strategy for the control of this disease. Previous studies have shown that Alternaria solani is the main early blight pathogen parasitizing potato. Here, we analyzed genetic and phenotypic diversity in isolates of Alternaria spp. covering all potato production areas in Serbia. We showed that the four species of Alternaria were found in areas with different distributions of the species. The occurrence of Alternaria spp. was studied by analyzing isolates from symptomatic potato leaves during multiyear sampling. In addition to Alternaria solani, we detected three more large-spored species identified as A. linariae (syn. A. tomatophila), A. protenta, and A. grandis that were involved in early blight disease on naturally infected potato leaves in Serbia. Differentiation of species was supported by phylogeny obtained from the DNA sequences of the GAPDH, calmodulin and Rpb2 genes. Our findings present a new perspective into the population structure of large-spored Alternaria species associated with early blight disease. Within the groups of large-spored Alternaria present in Serbia, evidence of A. protenta at high frequency reveals new insight into the contribution of Alternaria species in early blight disease. This work opens new perspectives for early blight management, while the distribution of different species on the same host suggests that the etiology of disease could depend on crop organization and the presence of other Alternaria hosts in close proximity to potato plants.",
publisher = "Frontiers Media",
journal = "Frontiers in Microbiology",
title = "New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia",
number = "856898",
volume = "13",
doi = "10.3389/fmicb.2022.856898"
}

Ivanović, Ž., Blagojević, J., Jovanović, G., Ivanović, B.,& Žeželj, D.. (2022). New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia. in Frontiers in Microbiology
Frontiers Media., 13(856898).
https://doi.org/10.3389/fmicb.2022.856898

Ivanović Ž, Blagojević J, Jovanović G, Ivanović B, Žeželj D. New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia. in Frontiers in Microbiology. 2022;13(856898).
doi:10.3389/fmicb.2022.856898 .

Ivanović, Žarko, Blagojević, Jovana, Jovanović, Gordana, Ivanović, Borko, Žeželj, Danica, "New Insight in the Occurrence of Early Blight Disease on Potato Reveals High Distribution of Alternaria solani and Alternaria protenta in Serbia" in Frontiers in Microbiology, 13, no. 856898 (2022),
https://doi.org/10.3389/fmicb.2022.856898 . .

TY  - JOUR
AU  - Einspanier, Severin
AU  - Susanto, Tamara
AU  - Metz, Nicole
AU  - Wolters, Pieter
AU  - Vleeshouwers, Vivianne
AU  - Lankinen, Åsa
AU  - Liljeroth, Erland
AU  - Landschoot, Sofie
AU  - Ivanović, Žarko
AU  - Huckelhoven, Ralph
AU  - Hausladen, Hans
AU  - Stam, Remco
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/745
AB  - Early blight of potato is caused by the fungal pathogen Alternaria solani and is an increasing problem worldwide. The primary strategy to control the disease is applying fungicides such as succinate dehydrogenase inhibitors (SDHI). SDHI-resistant strains, showing reduced sensitivity to treatments, appeared in Germany in 2013, shortly after the introduction of SDHIs. Two primary mutations in the SDH complex (SdhB-H278Y and SdhC-H134R) have been frequently found throughout Europe. How these resistances arose and spread, and whether they are linked to other genomic features, remains unknown. For this project, we performed whole-genome sequencing for 48 A. solani isolates from potato fields across Europe to better characterize the pathogen's genetic diversity in general and understand the development and spread of the genetic mutations that lead to SDHI resistance. The isolates can be grouped into seven genotypes. These genotypes do not show a geographical pattern but appear spread throughout Europe. We found clear evidence for recombination on the genome, and the observed admixtures might indicate a higher adaptive potential of the fungus than previously thought. Yet, we cannot link the observed recombination events to different Sdh mutations. The same Sdh mutations appear in different, non-admixed genetic backgrounds; therefore, we conclude they arose independently. Our research gives insights into the genetic diversity of A. solani on a genome level. The mixed occurrence of different genotypes, apparent admixture in the populations, and evidence for recombination indicate higher genomic complexity than anticipated. The conclusion that SDHI tolerance arose multiple times independently has important implications for future fungicide resistance management strategies. These should not solely focus on preventing the spread of isolates between locations but also on limiting population size and the selective pressure posed by fungicides in a given field to avoid the rise of new mutations in other genetic backgrounds.
PB  - Wiley
T2  - Evolutionary Applications
T1  - Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.
EP  - 1620
IS  - 10
SP  - 1605
VL  - 15
DO  - 10.1111/eva.13350
ER  - 

@article{
author = "Einspanier, Severin and Susanto, Tamara and Metz, Nicole and Wolters, Pieter and Vleeshouwers, Vivianne and Lankinen, Åsa and Liljeroth, Erland and Landschoot, Sofie and Ivanović, Žarko and Huckelhoven, Ralph and Hausladen, Hans and Stam, Remco",
year = "2022",
abstract = "Early blight of potato is caused by the fungal pathogen Alternaria solani and is an increasing problem worldwide. The primary strategy to control the disease is applying fungicides such as succinate dehydrogenase inhibitors (SDHI). SDHI-resistant strains, showing reduced sensitivity to treatments, appeared in Germany in 2013, shortly after the introduction of SDHIs. Two primary mutations in the SDH complex (SdhB-H278Y and SdhC-H134R) have been frequently found throughout Europe. How these resistances arose and spread, and whether they are linked to other genomic features, remains unknown. For this project, we performed whole-genome sequencing for 48 A. solani isolates from potato fields across Europe to better characterize the pathogen's genetic diversity in general and understand the development and spread of the genetic mutations that lead to SDHI resistance. The isolates can be grouped into seven genotypes. These genotypes do not show a geographical pattern but appear spread throughout Europe. We found clear evidence for recombination on the genome, and the observed admixtures might indicate a higher adaptive potential of the fungus than previously thought. Yet, we cannot link the observed recombination events to different Sdh mutations. The same Sdh mutations appear in different, non-admixed genetic backgrounds; therefore, we conclude they arose independently. Our research gives insights into the genetic diversity of A. solani on a genome level. The mixed occurrence of different genotypes, apparent admixture in the populations, and evidence for recombination indicate higher genomic complexity than anticipated. The conclusion that SDHI tolerance arose multiple times independently has important implications for future fungicide resistance management strategies. These should not solely focus on preventing the spread of isolates between locations but also on limiting population size and the selective pressure posed by fungicides in a given field to avoid the rise of new mutations in other genetic backgrounds.",
publisher = "Wiley",
journal = "Evolutionary Applications",
title = "Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.",
pages = "1620-1605",
number = "10",
volume = "15",
doi = "10.1111/eva.13350"
}

Einspanier, S., Susanto, T., Metz, N., Wolters, P., Vleeshouwers, V., Lankinen, Å., Liljeroth, E., Landschoot, S., Ivanović, Ž., Huckelhoven, R., Hausladen, H.,& Stam, R.. (2022). Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.. in Evolutionary Applications
Wiley., 15(10), 1605-1620.
https://doi.org/10.1111/eva.13350

Einspanier S, Susanto T, Metz N, Wolters P, Vleeshouwers V, Lankinen Å, Liljeroth E, Landschoot S, Ivanović Ž, Huckelhoven R, Hausladen H, Stam R. Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.. in Evolutionary Applications. 2022;15(10):1605-1620.
doi:10.1111/eva.13350 .

Einspanier, Severin, Susanto, Tamara, Metz, Nicole, Wolters, Pieter, Vleeshouwers, Vivianne, Lankinen, Åsa, Liljeroth, Erland, Landschoot, Sofie, Ivanović, Žarko, Huckelhoven, Ralph, Hausladen, Hans, Stam, Remco, "Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani." in Evolutionary Applications, 15, no. 10 (2022):1605-1620,
https://doi.org/10.1111/eva.13350 . .

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/685
AB  - Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Genetic Diversity of Pectobacterium spp. on Potato in Serbia
IS  - 9
VL  - 10
DO  - 10.3390/microorganisms10091840
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ivanović, Žarko and Ignjatov, Maja and Budakov, Dragana and Grahovac, Jovana and Grahovac, Mila",
year = "2022",
abstract = "Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Genetic Diversity of Pectobacterium spp. on Potato in Serbia",
number = "9",
volume = "10",
doi = "10.3390/microorganisms10091840"
}

Loc, M., Milošević, D., Ivanović, Ž., Ignjatov, M., Budakov, D., Grahovac, J.,& Grahovac, M.. (2022). Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms
MDPI., 10(9).
https://doi.org/10.3390/microorganisms10091840

Loc M, Milošević D, Ivanović Ž, Ignjatov M, Budakov D, Grahovac J, Grahovac M. Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms. 2022;10(9).
doi:10.3390/microorganisms10091840 .

Loc, Marta, Milošević, Dragana, Ivanović, Žarko, Ignjatov, Maja, Budakov, Dragana, Grahovac, Jovana, Grahovac, Mila, "Genetic Diversity of Pectobacterium spp. on Potato in Serbia" in Microorganisms, 10, no. 9 (2022),
https://doi.org/10.3390/microorganisms10091840 . .

TY  - JOUR
AU  - Savković, Željko
AU  - Stupar, Milos
AU  - Unković, Nikola
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Popović, Slađana
AU  - Vukojevic, Jelena
AU  - Ljaljević-Grbić, Milica
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/748
AB  - An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.
PB  - Elsevier
T2  - International Biodeterioration & Biodegradation
T1  - Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility
EP  - 163
SP  - 105
VL  - 157
DO  - 10.1016/j.ibiod.2020.105163
ER  - 

@article{
author = "Savković, Željko and Stupar, Milos and Unković, Nikola and Ivanović, Žarko and Blagojević, Jovana and Popović, Slađana and Vukojevic, Jelena and Ljaljević-Grbić, Milica",
year = "2021",
abstract = "An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.",
publisher = "Elsevier",
journal = "International Biodeterioration & Biodegradation",
title = "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility",
pages = "163-105",
volume = "157",
doi = "10.1016/j.ibiod.2020.105163"
}

Savković, Ž., Stupar, M., Unković, N., Ivanović, Ž., Blagojević, J., Popović, S., Vukojevic, J.,& Ljaljević-Grbić, M.. (2021). Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation
Elsevier., 157, 105-163.
https://doi.org/10.1016/j.ibiod.2020.105163

Savković Ž, Stupar M, Unković N, Ivanović Ž, Blagojević J, Popović S, Vukojevic J, Ljaljević-Grbić M. Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation. 2021;157:105-163.
doi:10.1016/j.ibiod.2020.105163 .

Savković, Željko, Stupar, Milos, Unković, Nikola, Ivanović, Žarko, Blagojević, Jovana, Popović, Slađana, Vukojevic, Jelena, Ljaljević-Grbić, Milica, "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility" in International Biodeterioration & Biodegradation, 157 (2021):105-163,
https://doi.org/10.1016/j.ibiod.2020.105163 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Marisavljević, Dragana
AU  - Marinković, Radovan
AU  - Mitrović, Petar
AU  - Blagojević, Jovana
AU  - Nikolić, Ivan
AU  - Pavlovic, Danijela
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/729
AB  - In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum span- ning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.
PB  - Korean Society of Plant Pathology
T2  - The Plant Pathology Journal
T1  - Genetic Diversity of Orobanche cumana Populations in Serbia
EP  - 520
IS  - 6
SP  - 512
VL  - 37
DO  - 10.5423/PPJ.OA.04.2021.0066
DO  - 1598-2254
ER  - 

@article{
author = "Ivanović, Žarko and Marisavljević, Dragana and Marinković, Radovan and Mitrović, Petar and Blagojević, Jovana and Nikolić, Ivan and Pavlovic, Danijela",
year = "2021",
abstract = "In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum span- ning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.",
publisher = "Korean Society of Plant Pathology",
journal = "The Plant Pathology Journal",
title = "Genetic Diversity of Orobanche cumana Populations in Serbia",
pages = "520-512",
number = "6",
volume = "37",
doi = "10.5423/PPJ.OA.04.2021.0066, 1598-2254"
}

Ivanović, Ž., Marisavljević, D., Marinković, R., Mitrović, P., Blagojević, J., Nikolić, I.,& Pavlovic, D.. (2021). Genetic Diversity of Orobanche cumana Populations in Serbia. in The Plant Pathology Journal
Korean Society of Plant Pathology., 37(6), 512-520.
https://doi.org/10.5423/PPJ.OA.04.2021.0066

Ivanović Ž, Marisavljević D, Marinković R, Mitrović P, Blagojević J, Nikolić I, Pavlovic D. Genetic Diversity of Orobanche cumana Populations in Serbia. in The Plant Pathology Journal. 2021;37(6):512-520.
doi:10.5423/PPJ.OA.04.2021.0066 .

Ivanović, Žarko, Marisavljević, Dragana, Marinković, Radovan, Mitrović, Petar, Blagojević, Jovana, Nikolić, Ivan, Pavlovic, Danijela, "Genetic Diversity of Orobanche cumana Populations in Serbia" in The Plant Pathology Journal, 37, no. 6 (2021):512-520,
https://doi.org/10.5423/PPJ.OA.04.2021.0066 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/625
AB  - Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot
EP  - 27
SP  - 21
VL  - 140
DO  - 10.2298/ZMSPN2140021I
ER  - 

@article{
author = "Ignjatov, Maja and Milošević, Dragana and Tamindžić, Gordana and Ivanović, Žarko",
year = "2021",
abstract = "Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot",
pages = "27-21",
volume = "140",
doi = "10.2298/ZMSPN2140021I"
}

Ignjatov, M., Milošević, D., Tamindžić, G.,& Ivanović, Ž.. (2021). Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 21-27.
https://doi.org/10.2298/ZMSPN2140021I

Ignjatov M, Milošević D, Tamindžić G, Ivanović Ž. Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot. in Zbornik Matice srpske za prirodne nauke. 2021;140:21-27.
doi:10.2298/ZMSPN2140021I .

Ignjatov, Maja, Milošević, Dragana, Tamindžić, Gordana, Ivanović, Žarko, "Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot" in Zbornik Matice srpske za prirodne nauke, 140 (2021):21-27,
https://doi.org/10.2298/ZMSPN2140021I . .

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Vukojevic, Jelena
AU  - Ivanović, Žarko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/750
AB  - The global demand for rapeseed makes it one of the fastest growing markets in crop production, with a need for increasing growing area and productivity, both of which depend on effective pathogen control strategies. Alternaria pathogens cause serious losses of brassica crops and occur in most rapeseed-growing regions around the world. In this study, morphological, molecular, and pathogenic analyses of 113 isolates collected from nine important rapeseed-growing areas in Serbia identified four pathogens: Alternaria brassicae, A. brassicicola, A. japonica, and A. alternata, causing leaf spot disease. Molecular analyses of ITS, GAPDH, Alt a1, and ATP sequences revealed one multilocus haplotype for A. brassicae and A. japonica isolates, whereas for A. brassicicola and A. alternata three and five haplotypes were distinguished, respectively. Pathogenicity tests showed that A. brassicicola was the most virulent while A. brassicae and A. japonica exhibited the same level of pathogenicity. The A. alternata population was generally weakly pathogenic with one nonpathogenic, genetically separated but closely related group of isolates, suggesting that pathogenicity is more unstable in this phylogenetic lineage. The data recorded on rate of growth and sporulation of isolates at 0, 5, 10, 15, 20, 25, 30, 35, and 40 °C revealed significant differences in evolutionary strategies among species, as A. alternata had the widest optimum range and the fastest growth rate, A. brassicicola showed the highest sporulation intensity, and A. brassicae expressed lower optimum temperatures for sporulation compared to other groups. All species indicated the potential for cross-infection of cabbage, and some haplotypes of A. brassicicola were previously isolated from horseradish in Serbia, suggesting the presence of one persistent Alternaria population on multiple brassica hosts in the region. This report describes the first detailed study of Alternaria spp. in rapeseed in Serbia.
PB  - British Society for Plant Pathology
T2  - Plant Pathology
T1  - Occurrence and characterization of Alternaria species associated with leaf spot disease in rapeseed in Serbia
EP  - 900
IS  - 5
SP  - 883
VL  - 69
DO  - 10.1111/ppa.13168
ER  - 

@article{
author = "Blagojević, Jovana and Vukojevic, Jelena and Ivanović, Žarko",
year = "2020",
abstract = "The global demand for rapeseed makes it one of the fastest growing markets in crop production, with a need for increasing growing area and productivity, both of which depend on effective pathogen control strategies. Alternaria pathogens cause serious losses of brassica crops and occur in most rapeseed-growing regions around the world. In this study, morphological, molecular, and pathogenic analyses of 113 isolates collected from nine important rapeseed-growing areas in Serbia identified four pathogens: Alternaria brassicae, A. brassicicola, A. japonica, and A. alternata, causing leaf spot disease. Molecular analyses of ITS, GAPDH, Alt a1, and ATP sequences revealed one multilocus haplotype for A. brassicae and A. japonica isolates, whereas for A. brassicicola and A. alternata three and five haplotypes were distinguished, respectively. Pathogenicity tests showed that A. brassicicola was the most virulent while A. brassicae and A. japonica exhibited the same level of pathogenicity. The A. alternata population was generally weakly pathogenic with one nonpathogenic, genetically separated but closely related group of isolates, suggesting that pathogenicity is more unstable in this phylogenetic lineage. The data recorded on rate of growth and sporulation of isolates at 0, 5, 10, 15, 20, 25, 30, 35, and 40 °C revealed significant differences in evolutionary strategies among species, as A. alternata had the widest optimum range and the fastest growth rate, A. brassicicola showed the highest sporulation intensity, and A. brassicae expressed lower optimum temperatures for sporulation compared to other groups. All species indicated the potential for cross-infection of cabbage, and some haplotypes of A. brassicicola were previously isolated from horseradish in Serbia, suggesting the presence of one persistent Alternaria population on multiple brassica hosts in the region. This report describes the first detailed study of Alternaria spp. in rapeseed in Serbia.",
publisher = "British Society for Plant Pathology",
journal = "Plant Pathology",
title = "Occurrence and characterization of Alternaria species associated with leaf spot disease in rapeseed in Serbia",
pages = "900-883",
number = "5",
volume = "69",
doi = "10.1111/ppa.13168"
}

Blagojević, J., Vukojevic, J.,& Ivanović, Ž.. (2020). Occurrence and characterization of Alternaria species associated with leaf spot disease in rapeseed in Serbia. in Plant Pathology
British Society for Plant Pathology., 69(5), 883-900.
https://doi.org/10.1111/ppa.13168

Blagojević J, Vukojevic J, Ivanović Ž. Occurrence and characterization of Alternaria species associated with leaf spot disease in rapeseed in Serbia. in Plant Pathology. 2020;69(5):883-900.
doi:10.1111/ppa.13168 .

Blagojević, Jovana, Vukojevic, Jelena, Ivanović, Žarko, "Occurrence and characterization of Alternaria species associated with leaf spot disease in rapeseed in Serbia" in Plant Pathology, 69, no. 5 (2020):883-900,
https://doi.org/10.1111/ppa.13168 . .

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Vukojevic, Jelena
AU  - Ivanović, Borko
AU  - Ivanović, Žarko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/749
AB  - Leaf spot diseases caused by Alternaria species have been reported worldwide in plants in the Brassicaceae family. However, there is little information on Alternaria species causing diseases in horseradish. In the present study, 89 Alternaria spp. isolates from Armoracia rusticana, sampled from nine districts in Serbia, were characterized based on their morphology, physiology, and molecular markers. Morphological characterization and molecular analyses based on ITS, GAPDH, Alt a 1, and PM-ATP sequences identified three distinct species associated with the disease: Alternaria brassicae, A. brassicicola, and A. alternata. For all species, growth and sporulation rates at 0, 5, 10, 15, 20, 25, 30, 35, and 40°C showed a quadratic response, with A. alternata having the widest temperature optimum (20 to 30°C) while A. brassicicola had higher optimum temperatures (20 to 25°C) than A. brassicae (15 to 20°C). To gain a better understanding of the pathogenicity of these species, the influence of leaf age, host susceptibility, and ability to infect artificially wounded and nonwounded leaves were tested. The pathogenicity test identified A. brassicicola and A. brassicae as the main causal agents of horseradish leaf spot disease. Results indicated that young and intact leaves of horseradish and cabbage were less susceptible to infection and also suggested the potential for cross-infection between these two hosts. Haplotype networks showed haplotype uniformity for A. brassicae, two haplotype groups of A. brassicicola, and eight haplotype groups of A. alternata in Serbia and suggest the possible association of some haplotypes with the geographic area. This study is the first to investigate Alternaria leaf spot disease on A. rusticana in Serbia and is the first record of A. brassicicola on horseradish in this country.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia
EP  - 1389
IS  - 5
SP  - 1378
VL  - 104
DO  - 10.1094/pdis-02-19-0289-re
DO  - 0191-2917
ER  - 

@article{
author = "Blagojević, Jovana and Vukojevic, Jelena and Ivanović, Borko and Ivanović, Žarko",
year = "2020",
abstract = "Leaf spot diseases caused by Alternaria species have been reported worldwide in plants in the Brassicaceae family. However, there is little information on Alternaria species causing diseases in horseradish. In the present study, 89 Alternaria spp. isolates from Armoracia rusticana, sampled from nine districts in Serbia, were characterized based on their morphology, physiology, and molecular markers. Morphological characterization and molecular analyses based on ITS, GAPDH, Alt a 1, and PM-ATP sequences identified three distinct species associated with the disease: Alternaria brassicae, A. brassicicola, and A. alternata. For all species, growth and sporulation rates at 0, 5, 10, 15, 20, 25, 30, 35, and 40°C showed a quadratic response, with A. alternata having the widest temperature optimum (20 to 30°C) while A. brassicicola had higher optimum temperatures (20 to 25°C) than A. brassicae (15 to 20°C). To gain a better understanding of the pathogenicity of these species, the influence of leaf age, host susceptibility, and ability to infect artificially wounded and nonwounded leaves were tested. The pathogenicity test identified A. brassicicola and A. brassicae as the main causal agents of horseradish leaf spot disease. Results indicated that young and intact leaves of horseradish and cabbage were less susceptible to infection and also suggested the potential for cross-infection between these two hosts. Haplotype networks showed haplotype uniformity for A. brassicae, two haplotype groups of A. brassicicola, and eight haplotype groups of A. alternata in Serbia and suggest the possible association of some haplotypes with the geographic area. This study is the first to investigate Alternaria leaf spot disease on A. rusticana in Serbia and is the first record of A. brassicicola on horseradish in this country.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia",
pages = "1389-1378",
number = "5",
volume = "104",
doi = "10.1094/pdis-02-19-0289-re, 0191-2917"
}

Blagojević, J., Vukojevic, J., Ivanović, B.,& Ivanović, Ž.. (2020). Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia. in Plant Disease
American Phytopathological Society., 104(5), 1378-1389.
https://doi.org/10.1094/pdis-02-19-0289-re

Blagojević J, Vukojevic J, Ivanović B, Ivanović Ž. Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia. in Plant Disease. 2020;104(5):1378-1389.
doi:10.1094/pdis-02-19-0289-re .

Blagojević, Jovana, Vukojevic, Jelena, Ivanović, Borko, Ivanović, Žarko, "Characterization of Alternaria Species Associated with Leaf Spot Disease of Armoracia rusticana in Serbia" in Plant Disease, 104, no. 5 (2020):1378-1389,
https://doi.org/10.1094/pdis-02-19-0289-re . .

Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Stojanović, M., Popović, V.,& Ivanović, Ž.. (2020). First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease, 104(6), 1864-1864.
https://doi.org/10.1094/PDIS-10-19-2218-PDN

Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Stojanović M, Popović V, Ivanović Ž. First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease. 2020;104(6):1864-1864.
doi:10.1094/PDIS-10-19-2218-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Stojanović, Milan, Popović, Vera, Ivanović, Žarko, "First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia" in Plant Disease, 104, no. 6 (2020):1864-1864,
https://doi.org/10.1094/PDIS-10-19-2218-PDN . .

Ivanović, Ž., Milošević, D., Ignjatov, M., Marjanovic Jeromela, A., Karaman, M.,& Grahovac, M.. (2020). First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease
American Phytopathological Society., 104(9), 2516-2516.
https://doi.org/10.1094/PDIS-03-20-0602-PDN

Ivanović Ž, Milošević D, Ignjatov M, Marjanovic Jeromela A, Karaman M, Grahovac M. First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease. 2020;104(9):2516-2516.
doi:10.1094/PDIS-03-20-0602-PDN .

Ivanović, Žarko, Milošević, Dragana, Ignjatov, Maja, Marjanovic Jeromela, Ana, Karaman, Maja, Grahovac, Mila, "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia" in Plant Disease, 104, no. 9 (2020):2516-2516,
https://doi.org/10.1094/PDIS-03-20-0602-PDN . .

Blagojević, J., Janjatović, S., Ignjatov, M., Trkulja, N., Gašić, K.,& Ivanović, Ž.. (2020). First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia. in Plant Disease
American Phytopathological Society., 104(3), 986.
https://doi.org/10.1094/PDIS-06-19-1335-PDN

Blagojević J, Janjatović S, Ignjatov M, Trkulja N, Gašić K, Ivanović Ž. First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia. in Plant Disease. 2020;104(3):986.
doi:10.1094/PDIS-06-19-1335-PDN .

Blagojević, Jovana, Janjatović, S., Ignjatov, Maja, Trkulja, Nenad, Gašić, Katarina, Ivanović, Žarko, "First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia" in Plant Disease, 104, no. 3 (2020):986,
https://doi.org/10.1094/PDIS-06-19-1335-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/608
AB  - Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.
AB  - Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L
T1  - Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L
IS  - 137
SP  - 49
DO  - 10.2298/ZMSPN1937049I
ER  - 

@article{
author = "Ignjatov, Maja and Vlajić, Slobodan and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2019",
abstract = "Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves., Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L, Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L",
number = "137",
pages = "49",
doi = "10.2298/ZMSPN1937049I"
}

Ignjatov, M., Vlajić, S., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2019). Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(137), 49.
https://doi.org/10.2298/ZMSPN1937049I

Ignjatov M, Vlajić S, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke. 2019;(137):49.
doi:10.2298/ZMSPN1937049I .

Ignjatov, Maja, Vlajić, Slobodan, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L" in Zbornik Matice srpske za prirodne nauke, no. 137 (2019):49,
https://doi.org/10.2298/ZMSPN1937049I . .

TY  - JOUR
AU  - Savković, Zeljko
AU  - Stupar, Milos
AU  - Unković, Nikola
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Vukojević, Jelena
AU  - Ljaljević-Grbić, Milica
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/568
AB  - The study addresses in vitro degradation potential of airborne Aspergillus and Penicillium/Talarmyces species originating from cultural heritage conservation premises. A series of rapid, cost effective biodegradation assays were performed to assess production of extracellular pigments, acids, and enzymes. Most of the isolates have demonstrated positive growth in at least one of the preformed tests. Strongest overall degradation potential was demonstrated for Penicillium brevicompactum, P. glabrum, and Talaromyces sayulitensis while Aspergillus domesticus, A. penicillioides, A. pseudoglaucus, and A. ruber did not exhibit positive reaction in any of the employed assays. Majority of isolates exhibited proteolytic and cellulolytic activity while carbonate dissolution was observed for only five tested fungi. Highest alteration of pH value in liquid media was documented for T. sayulitensis while A. niger and P. expansum exhibited strongest acid production on CREA. Certain isolates, mostly Penicillium species, displayed production of extracellular pigments. The results imply that many of the tested fungi have significant biodegradation capacity, indicating their potential to inflict structural and esthetic alterations on cultural heritage objects.
PB  - Springer Heidelberg, Heidelberg
T2  - Science of Nature
T1  - In vitro biodegradation potential of airborne Aspergilli and Penicillia
IS  - 3-4
VL  - 106
DO  - 10.1007/s00114-019-1603-3
ER  - 

@article{
author = "Savković, Zeljko and Stupar, Milos and Unković, Nikola and Ivanović, Žarko and Blagojević, Jovana and Vukojević, Jelena and Ljaljević-Grbić, Milica",
year = "2019",
abstract = "The study addresses in vitro degradation potential of airborne Aspergillus and Penicillium/Talarmyces species originating from cultural heritage conservation premises. A series of rapid, cost effective biodegradation assays were performed to assess production of extracellular pigments, acids, and enzymes. Most of the isolates have demonstrated positive growth in at least one of the preformed tests. Strongest overall degradation potential was demonstrated for Penicillium brevicompactum, P. glabrum, and Talaromyces sayulitensis while Aspergillus domesticus, A. penicillioides, A. pseudoglaucus, and A. ruber did not exhibit positive reaction in any of the employed assays. Majority of isolates exhibited proteolytic and cellulolytic activity while carbonate dissolution was observed for only five tested fungi. Highest alteration of pH value in liquid media was documented for T. sayulitensis while A. niger and P. expansum exhibited strongest acid production on CREA. Certain isolates, mostly Penicillium species, displayed production of extracellular pigments. The results imply that many of the tested fungi have significant biodegradation capacity, indicating their potential to inflict structural and esthetic alterations on cultural heritage objects.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Science of Nature",
title = "In vitro biodegradation potential of airborne Aspergilli and Penicillia",
number = "3-4",
volume = "106",
doi = "10.1007/s00114-019-1603-3"
}

Savković, Z., Stupar, M., Unković, N., Ivanović, Ž., Blagojević, J., Vukojević, J.,& Ljaljević-Grbić, M.. (2019). In vitro biodegradation potential of airborne Aspergilli and Penicillia. in Science of Nature
Springer Heidelberg, Heidelberg., 106(3-4).
https://doi.org/10.1007/s00114-019-1603-3

Savković Z, Stupar M, Unković N, Ivanović Ž, Blagojević J, Vukojević J, Ljaljević-Grbić M. In vitro biodegradation potential of airborne Aspergilli and Penicillia. in Science of Nature. 2019;106(3-4).
doi:10.1007/s00114-019-1603-3 .

Savković, Zeljko, Stupar, Milos, Unković, Nikola, Ivanović, Žarko, Blagojević, Jovana, Vukojević, Jelena, Ljaljević-Grbić, Milica, "In vitro biodegradation potential of airborne Aspergilli and Penicillia" in Science of Nature, 106, no. 3-4 (2019),
https://doi.org/10.1007/s00114-019-1603-3 . .

TY  - JOUR
AU  - Ćilerdžić, Jasmina Lj.
AU  - Vukojević, Jelena
AU  - Klaus, Anita S.
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Stajić, Mirjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/555
AB  - Demand for Ganoderma lucidum basidiocarps on the global market is increased due to its numerous health benefits. As they are rare in nature and traditional cultivation on logs is not ecologically and economically justified method, current trend is finding of good alternative substrate for production. Whether wheat straw, the most abundant crop residue in Europe, could be a novel substrate for G. lucidum cultivation was the question which led to the definition of the goals. Two wild and one commercial strains were objects of the study. Despite some morphological differences among basidiocarps, all strains belong to G. lucidum sensu stricto, which was confirmed by analyses of ITS, tef1-a and rpb2 gene sequences. Wheat straw showed as a good substrate, namely the periods required for the complete colonisation of wheat straw by mycelium as well as the formation of primordia and basidiocarps were relatively short. The totally fresh and dry yield and biological efficiency were also significant. If it is taken into consideration that untreated wheat straw was used, validities of its introduction in industrial-scale G. lucidum cultivation can be demonstrated.
PB  - Wydawnictwo Akad Rolniczej W Lublinie, Lublin
T2  - Acta Scientiarum Polonorum-Hortorum Cultus
T1  - Wheat straw: A promissing substrate for ganoderma lucidum cultivation
EP  - 22
IS  - 1
SP  - 13
VL  - 17
DO  - 10.24326/asphc.2018.1.2
ER  - 

@article{
author = "Ćilerdžić, Jasmina Lj. and Vukojević, Jelena and Klaus, Anita S. and Ivanović, Žarko and Blagojević, Jovana and Stajić, Mirjana",
year = "2018",
abstract = "Demand for Ganoderma lucidum basidiocarps on the global market is increased due to its numerous health benefits. As they are rare in nature and traditional cultivation on logs is not ecologically and economically justified method, current trend is finding of good alternative substrate for production. Whether wheat straw, the most abundant crop residue in Europe, could be a novel substrate for G. lucidum cultivation was the question which led to the definition of the goals. Two wild and one commercial strains were objects of the study. Despite some morphological differences among basidiocarps, all strains belong to G. lucidum sensu stricto, which was confirmed by analyses of ITS, tef1-a and rpb2 gene sequences. Wheat straw showed as a good substrate, namely the periods required for the complete colonisation of wheat straw by mycelium as well as the formation of primordia and basidiocarps were relatively short. The totally fresh and dry yield and biological efficiency were also significant. If it is taken into consideration that untreated wheat straw was used, validities of its introduction in industrial-scale G. lucidum cultivation can be demonstrated.",
publisher = "Wydawnictwo Akad Rolniczej W Lublinie, Lublin",
journal = "Acta Scientiarum Polonorum-Hortorum Cultus",
title = "Wheat straw: A promissing substrate for ganoderma lucidum cultivation",
pages = "22-13",
number = "1",
volume = "17",
doi = "10.24326/asphc.2018.1.2"
}

Ćilerdžić, J. Lj., Vukojević, J., Klaus, A. S., Ivanović, Ž., Blagojević, J.,& Stajić, M.. (2018). Wheat straw: A promissing substrate for ganoderma lucidum cultivation. in Acta Scientiarum Polonorum-Hortorum Cultus
Wydawnictwo Akad Rolniczej W Lublinie, Lublin., 17(1), 13-22.
https://doi.org/10.24326/asphc.2018.1.2

Ćilerdžić JL, Vukojević J, Klaus AS, Ivanović Ž, Blagojević J, Stajić M. Wheat straw: A promissing substrate for ganoderma lucidum cultivation. in Acta Scientiarum Polonorum-Hortorum Cultus. 2018;17(1):13-22.
doi:10.24326/asphc.2018.1.2 .

Ćilerdžić, Jasmina Lj., Vukojević, Jelena, Klaus, Anita S., Ivanović, Žarko, Blagojević, Jovana, Stajić, Mirjana, "Wheat straw: A promissing substrate for ganoderma lucidum cultivation" in Acta Scientiarum Polonorum-Hortorum Cultus, 17, no. 1 (2018):13-22,
https://doi.org/10.24326/asphc.2018.1.2 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Nikolić, Ivan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/551
AB  - During the spring of 2014 and 2015, a leaf spot disease was observed on heart-leaf philodendron (Philodendron scandens Koch & Sello) plants grown in a several house gardens near city of Jagodina, Moraviki region of Serbia. Diseased leaves were covered with watersoaked leaf spots with surrounded by yellow halos. Based on biochemical and molecular tests Pseudomonas syringae pv. syringae was identified as causing agent of bacterial leaf spot of heart-leaf philodendron and is in humid season accountable for appearence of disease. The genetic diversity within this pathogen isolated from different hosts in central Serbia, was studied to obtain insights into disease etiology. Therefore, kiwi fruit and fig tree leaves, with disease symptoms that were similar to those observed on heart-leaf philodendron plants, were collected and bacteria were isolated on NAS medium. Isolates were identified as P. syringae pv. syringae by detection of syrB gene, biochemical, pathogenicity tests and sequencing of gyrB gene. In cross inoculation tests on potted host plants, each strain induced leaf spot symptoms on heart-leaf philodendron and fig tree leaves, apart of the host of origin, and no significant symptoms in kiwi fruit leaves. Genomic profiles generated with rep-PCR analysis revealed that all strains originated from diseased heart-leaf philodendron, kiwi fruit and fig tree leaves had similiar DNA fingerprints. Overall, the data demonstrate P. syringae pv. syringae as a causing agent of leaf spot disease of heart-leaf philodendron and fig tree in Moraviki region of Serbia. This research is the first study that identified P. syringae pv. syringae associated with leaf spot disease on P. scandens, and also a starting point for investigation of the epidemiology of leaf spot disease on fruit trees that could be infected by the same bacterial strains.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Leaf spot disease on Philodendron scandens, Ficus carica and Actinidia deliciosa caused by Pseudomonas syringae pv. syringae in Serbia
EP  - 1113
IS  - 4
SP  - 1107
VL  - 151
DO  - 10.1007/s10658-018-1437-4
ER  - 

@article{
author = "Ivanović, Žarko and Blagojević, Jovana and Nikolić, Ivan",
year = "2018",
abstract = "During the spring of 2014 and 2015, a leaf spot disease was observed on heart-leaf philodendron (Philodendron scandens Koch & Sello) plants grown in a several house gardens near city of Jagodina, Moraviki region of Serbia. Diseased leaves were covered with watersoaked leaf spots with surrounded by yellow halos. Based on biochemical and molecular tests Pseudomonas syringae pv. syringae was identified as causing agent of bacterial leaf spot of heart-leaf philodendron and is in humid season accountable for appearence of disease. The genetic diversity within this pathogen isolated from different hosts in central Serbia, was studied to obtain insights into disease etiology. Therefore, kiwi fruit and fig tree leaves, with disease symptoms that were similar to those observed on heart-leaf philodendron plants, were collected and bacteria were isolated on NAS medium. Isolates were identified as P. syringae pv. syringae by detection of syrB gene, biochemical, pathogenicity tests and sequencing of gyrB gene. In cross inoculation tests on potted host plants, each strain induced leaf spot symptoms on heart-leaf philodendron and fig tree leaves, apart of the host of origin, and no significant symptoms in kiwi fruit leaves. Genomic profiles generated with rep-PCR analysis revealed that all strains originated from diseased heart-leaf philodendron, kiwi fruit and fig tree leaves had similiar DNA fingerprints. Overall, the data demonstrate P. syringae pv. syringae as a causing agent of leaf spot disease of heart-leaf philodendron and fig tree in Moraviki region of Serbia. This research is the first study that identified P. syringae pv. syringae associated with leaf spot disease on P. scandens, and also a starting point for investigation of the epidemiology of leaf spot disease on fruit trees that could be infected by the same bacterial strains.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Leaf spot disease on Philodendron scandens, Ficus carica and Actinidia deliciosa caused by Pseudomonas syringae pv. syringae in Serbia",
pages = "1113-1107",
number = "4",
volume = "151",
doi = "10.1007/s10658-018-1437-4"
}

Ivanović, Ž., Blagojević, J.,& Nikolić, I.. (2018). Leaf spot disease on Philodendron scandens, Ficus carica and Actinidia deliciosa caused by Pseudomonas syringae pv. syringae in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 151(4), 1107-1113.
https://doi.org/10.1007/s10658-018-1437-4

Ivanović Ž, Blagojević J, Nikolić I. Leaf spot disease on Philodendron scandens, Ficus carica and Actinidia deliciosa caused by Pseudomonas syringae pv. syringae in Serbia. in European Journal of Plant Pathology. 2018;151(4):1107-1113.
doi:10.1007/s10658-018-1437-4 .

Ivanović, Žarko, Blagojević, Jovana, Nikolić, Ivan, "Leaf spot disease on Philodendron scandens, Ficus carica and Actinidia deliciosa caused by Pseudomonas syringae pv. syringae in Serbia" in European Journal of Plant Pathology, 151, no. 4 (2018):1107-1113,
https://doi.org/10.1007/s10658-018-1437-4 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, Dragana
AU  - Ivanović, Žarko
AU  - Karaman, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/541
AB  - During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank.
AB  - Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia
T1  - Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji
EP  - 129
IS  - 3
SP  - 125
VL  - 55
DO  - 10.5937/RatPov1803125I
ER  - 

@article{
author = "Ignjatov, Maja and Milosević, Dragana and Ivanović, Žarko and Karaman, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica",
year = "2018",
abstract = "During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank., Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia, Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji",
pages = "129-125",
number = "3",
volume = "55",
doi = "10.5937/RatPov1803125I"
}

Ignjatov, M., Milosević, D., Ivanović, Ž., Karaman, M., Vlajić, S., Nikolić, Z.,& Gvozdanović-Varga, J.. (2018). Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 55(3), 125-129.
https://doi.org/10.5937/RatPov1803125I

Ignjatov M, Milosević D, Ivanović Ž, Karaman M, Vlajić S, Nikolić Z, Gvozdanović-Varga J. Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo. 2018;55(3):125-129.
doi:10.5937/RatPov1803125I .

Ignjatov, Maja, Milosević, Dragana, Ivanović, Žarko, Karaman, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia" in Ratarstvo i povrtarstvo, 55, no. 3 (2018):125-129,
https://doi.org/10.5937/RatPov1803125I . .

TY  - JOUR
AU  - Ćilerdžić, Jasmina Lj.
AU  - Galić, Milica
AU  - Ivanović, Žarko
AU  - Brčeski, Ilija
AU  - Vukojević, Jelena
AU  - Stajić, Mirjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/530
AB  - Biological pretreatment of the lignocellulosic residues, in which white-rot fungi have a crucial role, has many advantages compared to the chemical, physical, and physico-chemical methods of delignification and therefore attracts increasing scientific attention. Regarding the fact that properties and capacities of the ligninolytic enzymes of Daedaleopsis spp. are still unknown, the aim of this study was to research how nitrogen sources and inducers affect the potential of Daedaleopsis confragosa and Daedaleopsis tricolor to degrade cherry sawdust. NH4NO3, (NH4)2SO4, and peptone were tested as nitrogen sources, while veratryl alcohol, p-anisidine, vanillic acid, and phenylmethylsulfonyl fluoride were the studied inducers. As Mn-dependent peroxidase and laccase were the leader enzymes and cherry sawdust/peptone medium the best stimulator of their activities, the effect of inducers on delignification potential of these species was studied during fermentation of that substrate. Veratryl alcohol was the best stimulator of laccase and phenylmethylsulfonyl fluoride of Mn-dependent peroxidase activity (27,610.0 and 1338.4 U/L, respectively). These inducers also increased cherry sawdust delignification selectivity, particularly in D. tricolor in the presence of phenylmethylsulfonyl fluoride (lignin:hemicellulose:cellulose = 32.1%:0.9%:11.7%). Owing to the presented results, studied species could have an important role in the phase of lignocellulose pretreatment in various biotechnological processes.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor
DO  - 10.1007/s12010-018-2884-2
ER  - 

@article{
author = "Ćilerdžić, Jasmina Lj. and Galić, Milica and Ivanović, Žarko and Brčeski, Ilija and Vukojević, Jelena and Stajić, Mirjana",
year = "2018",
abstract = "Biological pretreatment of the lignocellulosic residues, in which white-rot fungi have a crucial role, has many advantages compared to the chemical, physical, and physico-chemical methods of delignification and therefore attracts increasing scientific attention. Regarding the fact that properties and capacities of the ligninolytic enzymes of Daedaleopsis spp. are still unknown, the aim of this study was to research how nitrogen sources and inducers affect the potential of Daedaleopsis confragosa and Daedaleopsis tricolor to degrade cherry sawdust. NH4NO3, (NH4)2SO4, and peptone were tested as nitrogen sources, while veratryl alcohol, p-anisidine, vanillic acid, and phenylmethylsulfonyl fluoride were the studied inducers. As Mn-dependent peroxidase and laccase were the leader enzymes and cherry sawdust/peptone medium the best stimulator of their activities, the effect of inducers on delignification potential of these species was studied during fermentation of that substrate. Veratryl alcohol was the best stimulator of laccase and phenylmethylsulfonyl fluoride of Mn-dependent peroxidase activity (27,610.0 and 1338.4 U/L, respectively). These inducers also increased cherry sawdust delignification selectivity, particularly in D. tricolor in the presence of phenylmethylsulfonyl fluoride (lignin:hemicellulose:cellulose = 32.1%:0.9%:11.7%). Owing to the presented results, studied species could have an important role in the phase of lignocellulose pretreatment in various biotechnological processes.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor",
doi = "10.1007/s12010-018-2884-2"
}

Ćilerdžić, J. Lj., Galić, M., Ivanović, Ž., Brčeski, I., Vukojević, J.,& Stajić, M.. (2018). Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor. in Applied Biochemistry and Biotechnology
Springer..
https://doi.org/10.1007/s12010-018-2884-2

Ćilerdžić JL, Galić M, Ivanović Ž, Brčeski I, Vukojević J, Stajić M. Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor. in Applied Biochemistry and Biotechnology. 2018;.
doi:10.1007/s12010-018-2884-2 .

Ćilerdžić, Jasmina Lj., Galić, Milica, Ivanović, Žarko, Brčeski, Ilija, Vukojević, Jelena, Stajić, Mirjana, "Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor" in Applied Biochemistry and Biotechnology (2018),
https://doi.org/10.1007/s12010-018-2884-2 . .

TY  - JOUR
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Marinković, Jelena
AU  - Spremo, Nemanja
AU  - Karaman, Maja
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1044
AB  - Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of indigenous Bacillus spp. isolated from soil
EP  - 269
SP  - 261
VL  - 133
DO  - 10.2298/ZMSPN1733261B
ER  - 

@article{
author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Spremo, Nemanja and Karaman, Maja and Nikolić, Zorica and Ivanović, Žarko",
year = "2017",
abstract = "Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of indigenous Bacillus spp. isolated from soil",
pages = "269-261",
volume = "133",
doi = "10.2298/ZMSPN1733261B"
}

Bjelić, D., Ignjatov, M., Marinković, J., Spremo, N., Karaman, M., Nikolić, Z.,& Ivanović, Ž.. (2017). Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 261-269.
https://doi.org/10.2298/ZMSPN1733261B

Bjelić D, Ignjatov M, Marinković J, Spremo N, Karaman M, Nikolić Z, Ivanović Ž. Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke. 2017;133:261-269.
doi:10.2298/ZMSPN1733261B .

Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Spremo, Nemanja, Karaman, Maja, Nikolić, Zorica, Ivanović, Žarko, "Antifungal activity of indigenous Bacillus spp. isolated from soil" in Zbornik Matice srpske za prirodne nauke, 133 (2017):261-269,
https://doi.org/10.2298/ZMSPN1733261B . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović Milovanović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1026
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
EP  - 17
SP  - 9
VL  - 132
DO  - 10.2298/ZMSPN1732009I
DO  - 0352-4906
ER  - 

@article{
author = "Ignjatov, Maja and Popović Milovanović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)",
pages = "17-9",
volume = "132",
doi = "10.2298/ZMSPN1732009I, 0352-4906"
}

Ignjatov, M., Popović Milovanović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 132, 9-17.
https://doi.org/10.2298/ZMSPN1732009I

Ignjatov M, Popović Milovanović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;132:9-17.
doi:10.2298/ZMSPN1732009I .

Ignjatov, Maja, Popović Milovanović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Gvozdanović-Varga, J., Marinković, J.,& Ivanović, Ž.. (2017). First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease
American Phytopathological Society., 101(6), 1047-1048.
https://doi.org/10.1094/PDIS-11-16-1625-PDN

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Gvozdanović-Varga J, Marinković J, Ivanović Ž. First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease. 2017;101(6):1047-1048.
doi:10.1094/PDIS-11-16-1625-PDN .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Gvozdanović-Varga, Jelica, Marinković, Jelena, Ivanović, Žarko, "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia" in Plant Disease, 101, no. 6 (2017):1047-1048,
https://doi.org/10.1094/PDIS-11-16-1625-PDN . .