TY  - JOUR
AU  - Đalović, Ivica
AU  - Mitrović, Petar
AU  - Trivan, Goran
AU  - Jelušić, Aleksandra
AU  - Pezo, Lato
AU  - Janić Hajnal, Elizabet
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1246
AB  - Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics
of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to
significant economic losses. The aim of this study was to determine biochemical changes in peony
(Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca.
Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural
network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains
originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill
strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical
parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins
contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions
and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation
between different chemical parameters and revealed a clear separation among the samples. Based on
the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP,
NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced
high r2 values (1.000 during the training period) and low SOS values. Developing an effective early
warning system for the detection of plant diseases in different plant species is critical for improving
crop yield and quality.
PB  - MDPI Basel
T2  - Horticulturae
T1  - The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach
IS  - 5
SP  - 426
VL  - 10
DO  - 10.3390/horticulturae10050426
ER  - 

@article{
author = "Đalović, Ivica and Mitrović, Petar and Trivan, Goran and Jelušić, Aleksandra and Pezo, Lato and Janić Hajnal, Elizabet and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics
of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to
significant economic losses. The aim of this study was to determine biochemical changes in peony
(Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca.
Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural
network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains
originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill
strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical
parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins
contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions
and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation
between different chemical parameters and revealed a clear separation among the samples. Based on
the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP,
NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced
high r2 values (1.000 during the training period) and low SOS values. Developing an effective early
warning system for the detection of plant diseases in different plant species is critical for improving
crop yield and quality.",
publisher = "MDPI Basel",
journal = "Horticulturae",
title = "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach",
number = "5",
pages = "426",
volume = "10",
doi = "10.3390/horticulturae10050426"
}

Đalović, I., Mitrović, P., Trivan, G., Jelušić, A., Pezo, L., Janić Hajnal, E.,& Popović Milovanović, T.. (2024). The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae
MDPI Basel., 10(5), 426.
https://doi.org/10.3390/horticulturae10050426

Đalović I, Mitrović P, Trivan G, Jelušić A, Pezo L, Janić Hajnal E, Popović Milovanović T. The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae. 2024;10(5):426.
doi:10.3390/horticulturae10050426 .

Đalović, Ivica, Mitrović, Petar, Trivan, Goran, Jelušić, Aleksandra, Pezo, Lato, Janić Hajnal, Elizabet, Popović Milovanović, Tatjana, "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach" in Horticulturae, 10, no. 5 (2024):426,
https://doi.org/10.3390/horticulturae10050426 . .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Trkulja, Nenad
AU  - Ristić, Danijela
AU  - Iličić, Renata
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1197
AB  - Bakteriozna pegavost lista šećerne repe prouzrokovana fitopatogenom bakterijom
Pseudomonas syringae pv. aptata (Psa) je široko rasprostranjena bolest, koja osim ove biljne
kulture napada blitvu, cveklu i dr. Ova bolest se poslednjih godina sve češće javlja na usevima
šećerne repe u Srbiji, posebno u uslovima prohladnog i vlažnog proleća. Epifitocije su
beležene 2013. godine u centralnim i južnim delovima Vojvodine (Bačka i Srem), kada su na
osnovu sekvenciranja konzervativnog gena gyrB, utvrđene četiri različite alelske varijante
ovog patogena. Obzirom na noviju epifitociju bakteriozne pegavosti lista na području gajenja
šećerne repe u Srbiji, cilj ovoga rada je bio da se izvrši izolacija i identifikacija prouzrokovača i
determiniše njegov genetički diverzitet na osnovu analize ponavljajućih palindromskih
sekvenci (rep-PCR) i nasumično amplifikovanih polimorfnih DNK (RAPD-PCR).
Tokom 2023. godine na šećernoj repi (sorta Viola) u lokalitetima Ruski Krstur i Srpski
Miletić prikupljeni su uzorci obolelog lišća sa simptomima bakteriozne pegavosti u vidu
nepravilnih nekrotičnih pega, oivičenih tamnijim marginama, sa centralnim delom mrke do
sive boje. U nekim slučajevima pege su se spajale čineći veću nekrotičnu leziju. Izolacija je
vršena na hranljivu podlogu obogaćenu sa 5% saharoze (NAS), na kojoj su nakon tri dana
inkubacije na 26 °C, dominirale beličaste, okrugle, sjajne i ispupčene bakterijske kolonije. Za
dalji rad je odabrano 18 izolata, a kao uporedni poslužili su referentni Psa sojevi, PD193 i
PD197. Prema rezultatima LOPAT testova, svi izolati su pokazali pripadnost Ia grupi
fluorescentnih Pseudomonas (+---+). Patogenost je dokazana infiltracijom bakterijske
suspenzije u list šećerne repe. Pripadnost izolata bakteriji Psa dokazana je PCR metodom
primenom prajmera specifičnih za patovar Papt2F/1R kojima su amplifikovani fragmenti DNK
veličine 250 bp karakteristični za Psa. Rep-PCR je izvođen primenom prajmera BOXA1R (BOXPCR), ERIC1R/ERIC2 (ERIC-PCR) i GTG5 (GTG5-PCR), dok je RAPD-PCR rađen primenom
prajmera M13 za M13-PCR. Na osnovu analize DNK profila, dobijenih primenom dve
pomenute metode, dokazano je postojanje tri (GTG5-PCR), odnosno četiri (BOX-, ERIC- i M13-
PCR) genetički različite grupe izolata. Dobijeni rezultati su ukazali na postojanje genetičkog
diverziteta i kod novijih populacija bakterije Psa poreklom sa šećerne repe. Daljom analizom
sekvenci više genskih lokusa (MLSA) moguće je detaljnije utvrditi eventualno postojanje
novih populacija ove bakterije u Srbiji.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova
T1  - Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe
SP  - 49
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Trkulja, Nenad and Ristić, Danijela and Iličić, Renata and Trkulja, Vojislav and Jelušić, Aleksandra",
year = "2023",
abstract = "Bakteriozna pegavost lista šećerne repe prouzrokovana fitopatogenom bakterijom
Pseudomonas syringae pv. aptata (Psa) je široko rasprostranjena bolest, koja osim ove biljne
kulture napada blitvu, cveklu i dr. Ova bolest se poslednjih godina sve češće javlja na usevima
šećerne repe u Srbiji, posebno u uslovima prohladnog i vlažnog proleća. Epifitocije su
beležene 2013. godine u centralnim i južnim delovima Vojvodine (Bačka i Srem), kada su na
osnovu sekvenciranja konzervativnog gena gyrB, utvrđene četiri različite alelske varijante
ovog patogena. Obzirom na noviju epifitociju bakteriozne pegavosti lista na području gajenja
šećerne repe u Srbiji, cilj ovoga rada je bio da se izvrši izolacija i identifikacija prouzrokovača i
determiniše njegov genetički diverzitet na osnovu analize ponavljajućih palindromskih
sekvenci (rep-PCR) i nasumično amplifikovanih polimorfnih DNK (RAPD-PCR).
Tokom 2023. godine na šećernoj repi (sorta Viola) u lokalitetima Ruski Krstur i Srpski
Miletić prikupljeni su uzorci obolelog lišća sa simptomima bakteriozne pegavosti u vidu
nepravilnih nekrotičnih pega, oivičenih tamnijim marginama, sa centralnim delom mrke do
sive boje. U nekim slučajevima pege su se spajale čineći veću nekrotičnu leziju. Izolacija je
vršena na hranljivu podlogu obogaćenu sa 5% saharoze (NAS), na kojoj su nakon tri dana
inkubacije na 26 °C, dominirale beličaste, okrugle, sjajne i ispupčene bakterijske kolonije. Za
dalji rad je odabrano 18 izolata, a kao uporedni poslužili su referentni Psa sojevi, PD193 i
PD197. Prema rezultatima LOPAT testova, svi izolati su pokazali pripadnost Ia grupi
fluorescentnih Pseudomonas (+---+). Patogenost je dokazana infiltracijom bakterijske
suspenzije u list šećerne repe. Pripadnost izolata bakteriji Psa dokazana je PCR metodom
primenom prajmera specifičnih za patovar Papt2F/1R kojima su amplifikovani fragmenti DNK
veličine 250 bp karakteristični za Psa. Rep-PCR je izvođen primenom prajmera BOXA1R (BOXPCR), ERIC1R/ERIC2 (ERIC-PCR) i GTG5 (GTG5-PCR), dok je RAPD-PCR rađen primenom
prajmera M13 za M13-PCR. Na osnovu analize DNK profila, dobijenih primenom dve
pomenute metode, dokazano je postojanje tri (GTG5-PCR), odnosno četiri (BOX-, ERIC- i M13-
PCR) genetički različite grupe izolata. Dobijeni rezultati su ukazali na postojanje genetičkog
diverziteta i kod novijih populacija bakterije Psa poreklom sa šećerne repe. Daljom analizom
sekvenci više genskih lokusa (MLSA) moguće je detaljnije utvrditi eventualno postojanje
novih populacija ove bakterije u Srbiji.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova",
title = "Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe",
pages = "49"
}

Popović Milovanović, T., Trkulja, N., Ristić, D., Iličić, R., Trkulja, V.,& Jelušić, A.. (2023). Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe. in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 49.

Popović Milovanović T, Trkulja N, Ristić D, Iličić R, Trkulja V, Jelušić A. Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe. in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova. 2023;:49..

Popović Milovanović, Tatjana, Trkulja, Nenad, Ristić, Danijela, Iličić, Renata, Trkulja, Vojislav, Jelušić, Aleksandra, "Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe" in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova (2023):49.

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1138
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
IS  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8, 2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8).
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8).
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023),
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Broćić, Zoran
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanisavljević, Rade
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/687
AB  - The aim of this study was to determine the influence of the origin and size of planting material on the morphological
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40
and 60 g, compared to the larger fraction of 80 g.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Influence of origin and size of potato planting material on morphological characteristics of seed tubers
EP  - 23
IS  - 1
SP  - 20
VL  - 25
DO  - 10.5937/jpea25-30772
DO  - 2956-0195
ER  - 

@article{
author = "Poštić, Dobrivoj and Štrbanović, Ratibor and Broćić, Zoran and Popović Milovanović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Stanisavljević, Rade",
year = "2021",
abstract = "The aim of this study was to determine the influence of the origin and size of planting material on the morphological
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40
and 60 g, compared to the larger fraction of 80 g.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Influence of origin and size of potato planting material on morphological characteristics of seed tubers",
pages = "23-20",
number = "1",
volume = "25",
doi = "10.5937/jpea25-30772, 2956-0195"
}

Poštić, D., Štrbanović, R., Broćić, Z., Popović Milovanović, T., Marković, S., Jelušić, A.,& Stanisavljević, R.. (2021). Influence of origin and size of potato planting material on morphological characteristics of seed tubers. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 25(1), 20-23.
https://doi.org/10.5937/jpea25-30772

Poštić D, Štrbanović R, Broćić Z, Popović Milovanović T, Marković S, Jelušić A, Stanisavljević R. Influence of origin and size of potato planting material on morphological characteristics of seed tubers. in Journal on Processing and Energy in Agriculture. 2021;25(1):20-23.
doi:10.5937/jpea25-30772 .

Poštić, Dobrivoj, Štrbanović, Ratibor, Broćić, Zoran, Popović Milovanović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Stanisavljević, Rade, "Influence of origin and size of potato planting material on morphological characteristics of seed tubers" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021):20-23,
https://doi.org/10.5937/jpea25-30772 . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/913
AB  - The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King’s B and crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, which produced characteristic pits on CVP medium were taken as representative. They were gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent on King’s B medium, levan and arginine dehydrolase negative. The isolates were able to cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. carotovorum. Further genetic characterization was based on sequence analysis of the gapA and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 (KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity among the cabbage isolates.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina
EP  - 222
IS  - 3-4
SP  - 211
VL  - 34
DO  - 10.2298/pif1904211p
DO  - 1820-3949
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Iličić, Renata",
year = "2019",
abstract = "The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King’s B and crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, which produced characteristic pits on CVP medium were taken as representative. They were gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent on King’s B medium, levan and arginine dehydrolase negative. The isolates were able to cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. carotovorum. Further genetic characterization was based on sequence analysis of the gapA and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 (KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity among the cabbage isolates.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina",
pages = "222-211",
number = "3-4",
volume = "34",
doi = "10.2298/pif1904211p, 1820-3949"
}

Popović Milovanović, T., Jelušić, A., Marković, S.,& Iličić, R.. (2019). Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 34(3-4), 211-222.
https://doi.org/10.2298/pif1904211p

Popović Milovanović T, Jelušić A, Marković S, Iličić R. Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina. in Pesticidi i fitomedicina. 2019;34(3-4):211-222.
doi:10.2298/pif1904211p .

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Iličić, Renata, "Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina" in Pesticidi i fitomedicina, 34, no. 3-4 (2019):211-222,
https://doi.org/10.2298/pif1904211p . .

Popović Milovanović, T., Jelušić, A., Milovanović, P., Janjatović, S., Budnar, M., Dimkić, I.,& Stanković, S.. (2017). First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia. in Plant Disease
American Phytopathological Society., 101(12), 2145-2146.
https://doi.org/10.1094/PDIS-05-17-0708-PDN

Popović Milovanović T, Jelušić A, Milovanović P, Janjatović S, Budnar M, Dimkić I, Stanković S. First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia. in Plant Disease. 2017;101(12):2145-2146.
doi:10.1094/PDIS-05-17-0708-PDN .

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Milovanović, Predrag, Janjatović, S., Budnar, M., Dimkić, Ivica, Stanković, Slaviša, "First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia" in Plant Disease, 101, no. 12 (2017):2145-2146,
https://doi.org/10.1094/PDIS-05-17-0708-PDN . .