TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1144
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}

Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN

Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .

TY  - JOUR
AU  - Božić, Vladimir
AU  - Vuković, Slavica
AU  - Grahovac, Mila
AU  - Lazić, Sanja
AU  - Aleksic, Goran
AU  - Šunjka, Dragana
PY  - 2021
UR  - https://enauka.gov.rs/handle/123456789/799170
UR  - https://ejfa.me/index.php/journal/article/view/2659
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1181
AB  - To minimize harmful effects of the fungicide application in sweet cherry orchards, it is necessary to use them in accordance with the good agricultural practice and to monitor presence of their residues. Cherry leaf spot caused by Blumeriella jaapii is a significant sweet cherry disease which control is heavily dependent on fungicide treatments. In this study, effects of fungicide treatments against B. jaapii and fungicide residues remaining in sweet cherry fruits after the treatments were evaluated, and the causal agent of cherry leaf spot was confirmed on cherry leaves from untreated control plots using conventional phytopathological techniques (isolation on nutrient media and morphological traits of developed fungal colonies). The trial was set up at two localities in south Serbia (District of Niš), in sweet cherry orchards, according to EPPO methods. Fungicides tested against B. jaapii were based on dodine (650 g a.i./kg) WP formulation, at concentration of 0.1% and mancozeb (800 g a.i./kg) WP formulation, at concentration of 0.25%. During the trial, two evaluations were carried out. Fungicide efficacy was determined according to Abbott. The obtained data were statistically processed by ANOVA and LSD test. In both sweet cherry orchards high efficacy in control B. jaapii was registered in case of dodine application efficacy of 96.3-98.9%, while mancozeb showed slightly lower efficacy of 91.0-95.6%. The results of the dissipation dynamic suggest that the dodine dissipation curves followed the first-order kinetic (Ct=6.23e−0.09, with R2=0.986) and its half-life in sweet cherry fruits was 7.7 days. The final residues in sweet cherry fruits were below the MRL (3 mg/kg) 21 days after the application. The results indicate that sweet cherry fruits can be safely consumed after dodine based fungicides applications at the recommended rate.
T2  - Emirates Journal of Food and Agriculture
T1  - Fungicide application and residues in control of Blumeriella jaapii (Rehm) Arx in sweet cherry
EP  - 259
IS  - 3
SP  - 253
VL  - 33
DO  - 10.9755/ejfa.2021.v33.i3.2659
ER  - 

@article{
author = "Božić, Vladimir and Vuković, Slavica and Grahovac, Mila and Lazić, Sanja and Aleksic, Goran and Šunjka, Dragana",
year = "2021",
abstract = "To minimize harmful effects of the fungicide application in sweet cherry orchards, it is necessary to use them in accordance with the good agricultural practice and to monitor presence of their residues. Cherry leaf spot caused by Blumeriella jaapii is a significant sweet cherry disease which control is heavily dependent on fungicide treatments. In this study, effects of fungicide treatments against B. jaapii and fungicide residues remaining in sweet cherry fruits after the treatments were evaluated, and the causal agent of cherry leaf spot was confirmed on cherry leaves from untreated control plots using conventional phytopathological techniques (isolation on nutrient media and morphological traits of developed fungal colonies). The trial was set up at two localities in south Serbia (District of Niš), in sweet cherry orchards, according to EPPO methods. Fungicides tested against B. jaapii were based on dodine (650 g a.i./kg) WP formulation, at concentration of 0.1% and mancozeb (800 g a.i./kg) WP formulation, at concentration of 0.25%. During the trial, two evaluations were carried out. Fungicide efficacy was determined according to Abbott. The obtained data were statistically processed by ANOVA and LSD test. In both sweet cherry orchards high efficacy in control B. jaapii was registered in case of dodine application efficacy of 96.3-98.9%, while mancozeb showed slightly lower efficacy of 91.0-95.6%. The results of the dissipation dynamic suggest that the dodine dissipation curves followed the first-order kinetic (Ct=6.23e−0.09, with R2=0.986) and its half-life in sweet cherry fruits was 7.7 days. The final residues in sweet cherry fruits were below the MRL (3 mg/kg) 21 days after the application. The results indicate that sweet cherry fruits can be safely consumed after dodine based fungicides applications at the recommended rate.",
journal = "Emirates Journal of Food and Agriculture",
title = "Fungicide application and residues in control of Blumeriella jaapii (Rehm) Arx in sweet cherry",
pages = "259-253",
number = "3",
volume = "33",
doi = "10.9755/ejfa.2021.v33.i3.2659"
}

Božić, V., Vuković, S., Grahovac, M., Lazić, S., Aleksic, G.,& Šunjka, D.. (2021). Fungicide application and residues in control of Blumeriella jaapii (Rehm) Arx in sweet cherry. in Emirates Journal of Food and Agriculture, 33(3), 253-259.
https://doi.org/10.9755/ejfa.2021.v33.i3.2659

Božić V, Vuković S, Grahovac M, Lazić S, Aleksic G, Šunjka D. Fungicide application and residues in control of Blumeriella jaapii (Rehm) Arx in sweet cherry. in Emirates Journal of Food and Agriculture. 2021;33(3):253-259.
doi:10.9755/ejfa.2021.v33.i3.2659 .

Božić, Vladimir, Vuković, Slavica, Grahovac, Mila, Lazić, Sanja, Aleksic, Goran, Šunjka, Dragana, "Fungicide application and residues in control of Blumeriella jaapii (Rehm) Arx in sweet cherry" in Emirates Journal of Food and Agriculture, 33, no. 3 (2021):253-259,
https://doi.org/10.9755/ejfa.2021.v33.i3.2659 . .

Ivanović, Ž., Milošević, D., Ignjatov, M., Marjanovic Jeromela, A., Karaman, M.,& Grahovac, M.. (2020). First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease
American Phytopathological Society., 104(9), 2516-2516.
https://doi.org/10.1094/PDIS-03-20-0602-PDN

Ivanović Ž, Milošević D, Ignjatov M, Marjanovic Jeromela A, Karaman M, Grahovac M. First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease. 2020;104(9):2516-2516.
doi:10.1094/PDIS-03-20-0602-PDN .

Ivanović, Žarko, Milošević, Dragana, Ignjatov, Maja, Marjanovic Jeromela, Ana, Karaman, Maja, Grahovac, Mila, "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia" in Plant Disease, 104, no. 9 (2020):2516-2516,
https://doi.org/10.1094/PDIS-03-20-0602-PDN . .

TY  - JOUR
AU  - Jankov, Dušan
AU  - Inđić, Dušanka
AU  - Kljajić, Petar
AU  - Almaši, Radmila
AU  - Andrić, Goran
AU  - Vuković, Slavica
AU  - Grahovac, Mila
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/938
AB  - The aim of the study was to investigate how various types of storage facilities with, e.g., concrete, metal, and plywood surfaces interfere with the activity of different insecticide formulations used for rice weevil Sitophilus oryzae (L.) control: malathion (EC), pirimiphos-methyl (EC), and lambda-cyhalothrin (CS and WP). Initial and residual efficacy were determined in the laboratory. Knockdown data for the initial effects were processed by probit analysis and presented as knockdown time (KDT) parameters with kdt-p lines. Delayed effects were shown as knockdown efficacy (%) determined after 24 h of weevils’ contact with 7-, 14-, 30-, 60-, 90-, 120-, 150-, and 180-day-old deposits on each surface. Malathion (EC) and pirimiphos-methyl (EC) showed the highest initial knockdown efficacy on metal, while it was 3.6 (3.4)- and 4.4 (3.3)-fold lower on concrete and plywood, respectively. Lambda-cyhalothrin (CS and WP) showed the highest initial efficacy on concrete, and slightly lower (1.3 and 2.4) fold on metal and plywood, respectively. Both formulations of lambda-cyhalothrin and malathion on metal, as well as pirimiphos-methyl on plywood were 100 % efficient against S. oryzae 180 days after the treatment. Delayed efficacy of both formulations of lambda-cyhalothrin decreased on plywood after 120 days, and after 180 days the efficacy was 55 %. All insecticides, except lambda-cyhalothrin (CS), expressed low knockdown efficacy on concrete, while the deposit of lambda-cyhalothrin (CS) on concrete was 100 % efficient during 90 days, and after 120, 150, and 180 days the efficacy was 83, 65, and 17 %, respectively.
T2  - Journal of Pest Science
T1  - Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.)
EP  - 216
SP  - 211
VL  - 86
DO  - 10.1007/s10340-012-0469-3
ER  - 

@article{
author = "Jankov, Dušan and Inđić, Dušanka and Kljajić, Petar and Almaši, Radmila and Andrić, Goran and Vuković, Slavica and Grahovac, Mila",
year = "2013",
abstract = "The aim of the study was to investigate how various types of storage facilities with, e.g., concrete, metal, and plywood surfaces interfere with the activity of different insecticide formulations used for rice weevil Sitophilus oryzae (L.) control: malathion (EC), pirimiphos-methyl (EC), and lambda-cyhalothrin (CS and WP). Initial and residual efficacy were determined in the laboratory. Knockdown data for the initial effects were processed by probit analysis and presented as knockdown time (KDT) parameters with kdt-p lines. Delayed effects were shown as knockdown efficacy (%) determined after 24 h of weevils’ contact with 7-, 14-, 30-, 60-, 90-, 120-, 150-, and 180-day-old deposits on each surface. Malathion (EC) and pirimiphos-methyl (EC) showed the highest initial knockdown efficacy on metal, while it was 3.6 (3.4)- and 4.4 (3.3)-fold lower on concrete and plywood, respectively. Lambda-cyhalothrin (CS and WP) showed the highest initial efficacy on concrete, and slightly lower (1.3 and 2.4) fold on metal and plywood, respectively. Both formulations of lambda-cyhalothrin and malathion on metal, as well as pirimiphos-methyl on plywood were 100 % efficient against S. oryzae 180 days after the treatment. Delayed efficacy of both formulations of lambda-cyhalothrin decreased on plywood after 120 days, and after 180 days the efficacy was 55 %. All insecticides, except lambda-cyhalothrin (CS), expressed low knockdown efficacy on concrete, while the deposit of lambda-cyhalothrin (CS) on concrete was 100 % efficient during 90 days, and after 120, 150, and 180 days the efficacy was 83, 65, and 17 %, respectively.",
journal = "Journal of Pest Science",
title = "Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.)",
pages = "216-211",
volume = "86",
doi = "10.1007/s10340-012-0469-3"
}

Jankov, D., Inđić, D., Kljajić, P., Almaši, R., Andrić, G., Vuković, S.,& Grahovac, M.. (2013). Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.). in Journal of Pest Science, 86, 211-216.
https://doi.org/10.1007/s10340-012-0469-3

Jankov D, Inđić D, Kljajić P, Almaši R, Andrić G, Vuković S, Grahovac M. Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.). in Journal of Pest Science. 2013;86:211-216.
doi:10.1007/s10340-012-0469-3 .

Jankov, Dušan, Inđić, Dušanka, Kljajić, Petar, Almaši, Radmila, Andrić, Goran, Vuković, Slavica, Grahovac, Mila, "Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.)" in Journal of Pest Science, 86 (2013):211-216,
https://doi.org/10.1007/s10340-012-0469-3 . .