TY  - JOUR
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Menković, Jelena
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/715
AB  - During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.
PB  - APS Publications
T2  - PLANT DISEASE
T1  - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
IS  - 3
VL  - 107
DO  - 10.1094/PDIS-06-22-1425-RE
ER  - 

@article{
author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2023",
abstract = "During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.",
publisher = "APS Publications",
journal = "PLANT DISEASE",
title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia",
number = "3",
volume = "107",
doi = "10.1094/PDIS-06-22-1425-RE"
}

Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in PLANT DISEASE
APS Publications., 107(3).
https://doi.org/10.1094/PDIS-06-22-1425-RE

Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in PLANT DISEASE. 2023;107(3).
doi:10.1094/PDIS-06-22-1425-RE .

Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in PLANT DISEASE, 107, no. 3 (2023),
https://doi.org/10.1094/PDIS-06-22-1425-RE . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zivkovic, Svetlana
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/744
AB  - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically
important pathogen of watermelon and related plant species worldwide. In the period 2014–2018,
several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of
43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic
and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene
sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four
housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR)
revealed that studied A. citrulli strains represent the homogeneous population and they clustered
together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR
based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity
of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in
their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon
varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay
revealed that the strains were able to infect other species within the Cucurbitaceae family.
PB  - MDPI
T2  - Agronomy
T1  - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
SP  - 235
VL  - 12
VL  - 2
DO  - 10.3390/agronomy12020235
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Zivkovic, Svetlana and Obradović, Aleksa",
year = "2022",
abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically
important pathogen of watermelon and related plant species worldwide. In the period 2014–2018,
several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of
43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic
and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene
sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four
housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR)
revealed that studied A. citrulli strains represent the homogeneous population and they clustered
together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR
based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity
of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in
their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon
varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay
revealed that the strains were able to infect other species within the Cucurbitaceae family.",
publisher = "MDPI",
journal = "Agronomy",
title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia",
pages = "235",
volume = "12, 2",
doi = "10.3390/agronomy12020235"
}

Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zivkovic, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy
MDPI., 12, 235.
https://doi.org/10.3390/agronomy12020235

Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zivkovic S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12:235.
doi:10.3390/agronomy12020235 .

Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Zivkovic, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12 (2022):235,
https://doi.org/10.3390/agronomy12020235 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Lela
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/725
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
IS  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Lela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
number = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, L.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12(803789).
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić L, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12(803789).
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Lela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12, no. 803789 (2022),
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - CHAP
AU  - Choudhary, Manoj
AU  - Paret, Mathews
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Jeff, Jones
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/763
AB  - Crop yield loss due to bacterial plant pathogens need to be reduced to increase global food production demand. Currently available disease management strategies involving copper-based bactericides and antibiotics are losing efficacy due to development of resistance in bacteria. There is long familiar demand of environmentally friendly and sustainable strategies to control bacterial diseases. Bacteriophages are virus that kill target bacteria without affecting another microorganism and environment. Bacteriophage efficiency on phyllosphere is mainly affected by ultraviolet (UV) light. Use of combination of phage, mixture with phage carrier bacteria and optimizing time of application helps in persistence of bacteriophage. There are several bacteriophage products already available in the market to control destructive bacterial diseases. Unlike chemical based traditional control measure, bacteriophage mixture can be easily amended to reduce resistance development in bacteria. In this chapter, the authors discuss from phage isolation to interaction with bacteria and control mechanism of plant diseases.
PB  - Burleigh Dodds Science Publishing
T2  - In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.
T1  - Bacteriophages for plant disease control
EP  - 506
SP  - 473
DO  - 10.19103/AS.2021.0093.18
ER  - 

@inbook{
author = "Choudhary, Manoj and Paret, Mathews and Obradović, Aleksa and Gašić, Katarina and Jeff, Jones",
year = "2022",
abstract = "Crop yield loss due to bacterial plant pathogens need to be reduced to increase global food production demand. Currently available disease management strategies involving copper-based bactericides and antibiotics are losing efficacy due to development of resistance in bacteria. There is long familiar demand of environmentally friendly and sustainable strategies to control bacterial diseases. Bacteriophages are virus that kill target bacteria without affecting another microorganism and environment. Bacteriophage efficiency on phyllosphere is mainly affected by ultraviolet (UV) light. Use of combination of phage, mixture with phage carrier bacteria and optimizing time of application helps in persistence of bacteriophage. There are several bacteriophage products already available in the market to control destructive bacterial diseases. Unlike chemical based traditional control measure, bacteriophage mixture can be easily amended to reduce resistance development in bacteria. In this chapter, the authors discuss from phage isolation to interaction with bacteria and control mechanism of plant diseases.",
publisher = "Burleigh Dodds Science Publishing",
journal = "In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.",
booktitle = "Bacteriophages for plant disease control",
pages = "506-473",
doi = "10.19103/AS.2021.0093.18"
}

Choudhary, M., Paret, M., Obradović, A., Gašić, K.,& Jeff, J.. (2022). Bacteriophages for plant disease control. in In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.
Burleigh Dodds Science Publishing., 473-506.
https://doi.org/10.19103/AS.2021.0093.18

Choudhary M, Paret M, Obradović A, Gašić K, Jeff J. Bacteriophages for plant disease control. in In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.. 2022;:473-506.
doi:10.19103/AS.2021.0093.18 .

Choudhary, Manoj, Paret, Mathews, Obradović, Aleksa, Gašić, Katarina, Jeff, Jones, "Bacteriophages for plant disease control" in In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506. (2022):473-506,
https://doi.org/10.19103/AS.2021.0093.18 . .

TY  - CONF
AU  - Obradović, Aleksa
AU  - Menković, Jelena
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1282
AB  - Glavni cilj istraživanja fitopatogenih bakterija je njihovo bolje poznavanje i razvoj
efikasne strategije zaštite. Klimatske promene, globalna distribucija reproduktivnog biljnog
materijala, evolucija poljoprivredne prakse i povećana zabrinutost za životnu sredinu,
zahtevaju nove pristupe kontroli bakterioza biljaka. Stoga je mogućnost primene prirodnih,
zdravstveno i ekološki prihvatljivih mehanizama kontrole fitopatogenih bakterija, izazvala
povećano interesovanje biljnih bakteriologa. Biološka kontrola, kao novi oslonac integrisanih
strategija kontrole biljnih bolesti, mogla bi doprineti prihvatljivoj efikasnosti i održivosti
trenutnih mera zaštite. Strategije biološke kontrole se uglavnom zasnivaju na sprečavanjju
nastanka infekcije, smanjenju učestalosti bakterioza biljaka bilo selektovanom ili
indukovanom otpornošću, konkurencijom, antibiozom ili ometanjem životnog ciklusa
patogena. Većina poznatih bioloških agenasa pripada rodovima Bacillus, Lactobacillus,
Erwinia, Pantoea, Streptomyces, Bdellovibrio, fluorescentnim Pseudomonas spp.,
bakteriofagima i protozoama. Neki takođe uključuju avirulentne oblike patogenih sojeva. Kao
jedna od ciljanih tačaka, osim samih bakterijskih ćelija, nameće se ometanje formiranja
biofilma ili manipulacija fizioloških procesa bakterije upotrebom signalnih molekula. Novija
istraživanja takođe ukazuju da bi se značajan napredak u ispoljavanju konkurentnosti i
sposobnosti bioloških agensa u proizvodnji antimikrobnih metabolita ili izazivanju otpornosti
biljaka mogao postići boljim razumevanjem prirode njihovog preživljavanja i procesa
interakcije sa fitopatogenim bakterijama, ali i sa drugim činiocima mikrobioma i životnom
sredinom uopšte. U većini slučajeva, efikasna primena takvih jedinjenja i zadovoljavajući
efekat njihove biološke aktivnosti zahtevaju razvoj posebnih tehnika ili kombinaciju više od
jednog agensa. Međutim, uprkos nedavno povećanom broju i raznolikosti istraživačkih
programa, mora se priznati da su broj i dostupnost komercijalnih biobaktericida i dalje
ograničeni.
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021
T1  - NOVI TRENDOVI U BIOLOŠKOJ KONTROLI FITOPATOGENIH BAKTERIJA
SP  - 9
ER  - 

@conference{
author = "Obradović, Aleksa and Menković, Jelena and Prokić, Anđelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina",
year = "2021",
abstract = "Glavni cilj istraživanja fitopatogenih bakterija je njihovo bolje poznavanje i razvoj
efikasne strategije zaštite. Klimatske promene, globalna distribucija reproduktivnog biljnog
materijala, evolucija poljoprivredne prakse i povećana zabrinutost za životnu sredinu,
zahtevaju nove pristupe kontroli bakterioza biljaka. Stoga je mogućnost primene prirodnih,
zdravstveno i ekološki prihvatljivih mehanizama kontrole fitopatogenih bakterija, izazvala
povećano interesovanje biljnih bakteriologa. Biološka kontrola, kao novi oslonac integrisanih
strategija kontrole biljnih bolesti, mogla bi doprineti prihvatljivoj efikasnosti i održivosti
trenutnih mera zaštite. Strategije biološke kontrole se uglavnom zasnivaju na sprečavanjju
nastanka infekcije, smanjenju učestalosti bakterioza biljaka bilo selektovanom ili
indukovanom otpornošću, konkurencijom, antibiozom ili ometanjem životnog ciklusa
patogena. Većina poznatih bioloških agenasa pripada rodovima Bacillus, Lactobacillus,
Erwinia, Pantoea, Streptomyces, Bdellovibrio, fluorescentnim Pseudomonas spp.,
bakteriofagima i protozoama. Neki takođe uključuju avirulentne oblike patogenih sojeva. Kao
jedna od ciljanih tačaka, osim samih bakterijskih ćelija, nameće se ometanje formiranja
biofilma ili manipulacija fizioloških procesa bakterije upotrebom signalnih molekula. Novija
istraživanja takođe ukazuju da bi se značajan napredak u ispoljavanju konkurentnosti i
sposobnosti bioloških agensa u proizvodnji antimikrobnih metabolita ili izazivanju otpornosti
biljaka mogao postići boljim razumevanjem prirode njihovog preživljavanja i procesa
interakcije sa fitopatogenim bakterijama, ali i sa drugim činiocima mikrobioma i životnom
sredinom uopšte. U većini slučajeva, efikasna primena takvih jedinjenja i zadovoljavajući
efekat njihove biološke aktivnosti zahtevaju razvoj posebnih tehnika ili kombinaciju više od
jednog agensa. Međutim, uprkos nedavno povećanom broju i raznolikosti istraživačkih
programa, mora se priznati da su broj i dostupnost komercijalnih biobaktericida i dalje
ograničeni.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021",
title = "NOVI TRENDOVI U BIOLOŠKOJ KONTROLI FITOPATOGENIH BAKTERIJA",
pages = "9"
}

Obradović, A., Menković, J., Prokić, A., Ivanović, M., Zlatković, N.,& Gašić, K.. (2021). NOVI TRENDOVI U BIOLOŠKOJ KONTROLI FITOPATOGENIH BAKTERIJA. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021
Društvo za zaštitu bilja., 9.

Obradović A, Menković J, Prokić A, Ivanović M, Zlatković N, Gašić K. NOVI TRENDOVI U BIOLOŠKOJ KONTROLI FITOPATOGENIH BAKTERIJA. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021. 2021;:9..

Obradović, Aleksa, Menković, Jelena, Prokić, Anđelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, "NOVI TRENDOVI U BIOLOŠKOJ KONTROLI FITOPATOGENIH BAKTERIJA" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021 (2021):9.

TY  - CHAP
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Menković, Jelena
AU  - Gašić, Katarina
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1281
AB  - Bakteriozna plamenjača jabučastih voćaka, koju prouzrokuje bakterija Erwinia
amylovora, predstavlja jednu od najštetnijih bakterioza na našim prostorima. Detekcija
bakterije u biljnom materijalu moguća je primenom lančanog umnožavanja specifičnih
fragmenata nukleinske kiseline (PCR) korišćenjem većeg broja setova specifičnih prajmera. U
cilju povećanja specifičnosti i brzine detekcije ovog patogena, razvijene su nove, osetljivije
metode kao što su „nested“ ili Real-time PCR. Jedna od najsavremenijih molekularnih tehnika
za detekciju E. amylovora je tzv. izotermna amplifikacija nukleniske kiseline posredstvom
petlje (Eng. Loop-Mediated Isothermal Amplification, LAMP). LAMP se zasniva na postulatima
konvencionalnog PCR-a i fluorimetrije uz upotrebu 3 para prajmera koji formiraju DNK
molekul nalik petlji, povećavajući specifičnost metode. Tokom reakcije, interkalirajuća boja
vezuje se za novosintetisane molekule DNK, što omogućava detekciju i relativnu kvantifikaciju
bakterijske DNK u uzorku u realnom vremenu. Najznačajnija prednost LAMP metode je
mogućnost umnožavanja specifičnih sekvenci pod izotermnim uslovima između 63 i 65 °C, pri
čemu ne dolazi do gubitka vremena u termičkim promenama, ujedno otklanjajući potrebu za
termosajklerom - uređajem neophodnim za ostale PCR tehnike.
U ovom radu, za detekciju E. amylovora u ekstraktu kruške i kontrolnoj suspenziji
bakterija različite koncentracije korišćen je prenosivi aparat ICGENE mini (Enbiotech, Italija), a
ekstrakcija i umnožavanje DNK obavljene su prema uputstvu proizvođača. Ova metoda koristi
šest prajmera koji detektuju vrlo konzerviranu sekvencu hromozomskog amsH gena, specifičnu
za E. amylovora. Unapred pripremljeni prajmeri nalaze se u liofilizovanom stanju u reakcionim
mikroepruvetama kako bi se smanjila mogućnost greške pipetiranjem tokom pripreme reakcije.
Osetljivost metode proučena je korišćenjem serije razređenja bakterijske suspenzije soja E.
amylovora KFB 159. Za proučavanje uticaja inhibitornih materija iz biljnog tkiva na osetljivost
metode korišćena je smeša bakterijske suspenzije i ekstrakta mladara kruške.
Primenom LAMP metode moguće je detektovati DNK E. amylovora u biljnom materijalu
za manje od 1h, dok je pomoću Real-time PCR-a potrebno oko 3h, a konvencionalnim PCR-om
preko 6h. U poređenju sa konvencionalnom PCR metodom, LAMP je jednostavnija i brža
metoda, pri čemu nema rukovanja PCR produktima, elektroforeze, bojenja i slikanja gela.
Rezultati pokazuju da je najniža detektovana koncentracija bakterija u ekstraktu kruške bila
1,38106 bakterija/ml, što je u poređenju sa drugim metodama nedovoljno osetljivo. Nešto
veća osetljivost zabeležena je u detekciji bakterija u kontrolnoj suspenziji bez biljnog
ekstrakta (2,3103 bakterija/ml). Rezultati ukazuju da je detekcija E. amylovora LAMP
metodom brza i jednostavna, ali da prisustvo biljnog ekstrakta značajno umanjuje osetljivost
metode. Negativan uticaj inhibitornih materija iz biljnog materijala se mora otkloniti
optimizacijom metode kako bi mogla da zameni postojeće PCR tehnike.
PB  - Društvo za zaštitu bilja
T2  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021
T1  - DETEKCIJA Erwinia amylovora PRIMENOM LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METODE
SP  - 47
ER  - 

@inbook{
author = "Ivanović, Milan and Prokić, Anđelka and Menković, Jelena and Gašić, Katarina and Zlatković, Nevena and Obradović, Aleksa",
year = "2021",
abstract = "Bakteriozna plamenjača jabučastih voćaka, koju prouzrokuje bakterija Erwinia
amylovora, predstavlja jednu od najštetnijih bakterioza na našim prostorima. Detekcija
bakterije u biljnom materijalu moguća je primenom lančanog umnožavanja specifičnih
fragmenata nukleinske kiseline (PCR) korišćenjem većeg broja setova specifičnih prajmera. U
cilju povećanja specifičnosti i brzine detekcije ovog patogena, razvijene su nove, osetljivije
metode kao što su „nested“ ili Real-time PCR. Jedna od najsavremenijih molekularnih tehnika
za detekciju E. amylovora je tzv. izotermna amplifikacija nukleniske kiseline posredstvom
petlje (Eng. Loop-Mediated Isothermal Amplification, LAMP). LAMP se zasniva na postulatima
konvencionalnog PCR-a i fluorimetrije uz upotrebu 3 para prajmera koji formiraju DNK
molekul nalik petlji, povećavajući specifičnost metode. Tokom reakcije, interkalirajuća boja
vezuje se za novosintetisane molekule DNK, što omogućava detekciju i relativnu kvantifikaciju
bakterijske DNK u uzorku u realnom vremenu. Najznačajnija prednost LAMP metode je
mogućnost umnožavanja specifičnih sekvenci pod izotermnim uslovima između 63 i 65 °C, pri
čemu ne dolazi do gubitka vremena u termičkim promenama, ujedno otklanjajući potrebu za
termosajklerom - uređajem neophodnim za ostale PCR tehnike.
U ovom radu, za detekciju E. amylovora u ekstraktu kruške i kontrolnoj suspenziji
bakterija različite koncentracije korišćen je prenosivi aparat ICGENE mini (Enbiotech, Italija), a
ekstrakcija i umnožavanje DNK obavljene su prema uputstvu proizvođača. Ova metoda koristi
šest prajmera koji detektuju vrlo konzerviranu sekvencu hromozomskog amsH gena, specifičnu
za E. amylovora. Unapred pripremljeni prajmeri nalaze se u liofilizovanom stanju u reakcionim
mikroepruvetama kako bi se smanjila mogućnost greške pipetiranjem tokom pripreme reakcije.
Osetljivost metode proučena je korišćenjem serije razređenja bakterijske suspenzije soja E.
amylovora KFB 159. Za proučavanje uticaja inhibitornih materija iz biljnog tkiva na osetljivost
metode korišćena je smeša bakterijske suspenzije i ekstrakta mladara kruške.
Primenom LAMP metode moguće je detektovati DNK E. amylovora u biljnom materijalu
za manje od 1h, dok je pomoću Real-time PCR-a potrebno oko 3h, a konvencionalnim PCR-om
preko 6h. U poređenju sa konvencionalnom PCR metodom, LAMP je jednostavnija i brža
metoda, pri čemu nema rukovanja PCR produktima, elektroforeze, bojenja i slikanja gela.
Rezultati pokazuju da je najniža detektovana koncentracija bakterija u ekstraktu kruške bila
1,38106 bakterija/ml, što je u poređenju sa drugim metodama nedovoljno osetljivo. Nešto
veća osetljivost zabeležena je u detekciji bakterija u kontrolnoj suspenziji bez biljnog
ekstrakta (2,3103 bakterija/ml). Rezultati ukazuju da je detekcija E. amylovora LAMP
metodom brza i jednostavna, ali da prisustvo biljnog ekstrakta značajno umanjuje osetljivost
metode. Negativan uticaj inhibitornih materija iz biljnog materijala se mora otkloniti
optimizacijom metode kako bi mogla da zameni postojeće PCR tehnike.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021",
booktitle = "DETEKCIJA Erwinia amylovora PRIMENOM LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METODE",
pages = "47"
}

Ivanović, M., Prokić, A., Menković, J., Gašić, K., Zlatković, N.,& Obradović, A.. (2021). DETEKCIJA Erwinia amylovora PRIMENOM LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METODE. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021
Društvo za zaštitu bilja., 47.

Ivanović M, Prokić A, Menković J, Gašić K, Zlatković N, Obradović A. DETEKCIJA Erwinia amylovora PRIMENOM LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METODE. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021. 2021;:47..

Ivanović, Milan, Prokić, Anđelka, Menković, Jelena, Gašić, Katarina, Zlatković, Nevena, Obradović, Aleksa, "DETEKCIJA Erwinia amylovora PRIMENOM LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METODE" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021 (2021):47.

TY  - CONF
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Blagojević, Nevena
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1279
AB  - Acidovorax citrulli, prouzrokovač mrljavosti plodova lubenice i dinje jedna je od
najdestruktivnijih bakterioza ovih biljaka u svetu. Patogen je detektovan u Srbiji 2014. godine,
kada je izazvao ogromne štete proizvodnji lubenice. Usled nedostatka efikasnih baktericida za
suzbijanje ovog patogena, nameće se potreba pronalaženja alternativnih rešenja. Stoga je
proučeno prisustvo bakteriofaga, prirodnih neprijatelja Acidovorax citrulli i izvršena njihova
izolacija i karakterizacija.
Tokom 2014. godine izolovano je dvanaest sojeva faga iz rizosfere obolelih biljaka
lubenice, kako i iz listova lubenice sa simptomima bakteriozne mrljavosti. Karakterizacija
sojeva izvršena je na osnovu spektra domaćina virusa, morfoloških odlika plakova i virusnih
čestica, termalne tačke inaktivacije, parametara umnožavanja faga, kao i RFLP analize.
Rezultati istraživanja pokazali su da svi sojevi faga liziraju 30 od ukupno 32 testirana
soja A. citrulli izolovana u Srbiji. Fagi su ispoljili vosoku specifičnost prema vrsti i nisu lizirali
ostale testirane vrste patogenih i saprofitnih bakterija. Svi sojevi formirali su prozračne
plakove, prečnika 2 mm, nakon 24 h inkubacije. Pomoću transmisionog elektronskog
mikrosopa, utvrđeno je da fagi pripadaju redu Caudovirales, familiji Siphoviridae. Tokom 20
min 90% faga se adsorbuje na površinu bakterijske ćelije, dok se po jednoj inficiranoj ćeliji
oslobodi prosečno 74 ± 5 čestice virusa. Termalna tačka inaktivacije faga iznosila je 66 ili
67ºC. Fagi su ispoljili osetljivost prema hloroformu, bili su stablini pri pH 5-9, ali inaktivirani
nakon 5-10 min izloženosti UV svetlu. RFLP analizom, korišćenjem EcoRI, BsmI and BamHI
enzima nisu utvrđene genetičke razlike među proučavanim sojevima faga.
Ova istraživanja stvaraju osnovu za dalji razvoj inovativnih metoda za kontrolu
bakteriozne mrljavosti lubenice primenom prirodnih mehanizama zaštite.
PB  - Društvo za zaštitu bilja
C3  - XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor
T1  - KARAKTERIZACIJA BAKTERIOFAGA SPECIFIČNIH PREMA Acidovorax citrulli – PROUZROKOVAČU MRLJAVOSTI PLODA LUBENICE
IS  - 17
ER  - 

@conference{
author = "Gašić, Katarina and Obradović, Mina and Blagojević, Nevena and Obradović, Aleksa",
year = "2021",
abstract = "Acidovorax citrulli, prouzrokovač mrljavosti plodova lubenice i dinje jedna je od
najdestruktivnijih bakterioza ovih biljaka u svetu. Patogen je detektovan u Srbiji 2014. godine,
kada je izazvao ogromne štete proizvodnji lubenice. Usled nedostatka efikasnih baktericida za
suzbijanje ovog patogena, nameće se potreba pronalaženja alternativnih rešenja. Stoga je
proučeno prisustvo bakteriofaga, prirodnih neprijatelja Acidovorax citrulli i izvršena njihova
izolacija i karakterizacija.
Tokom 2014. godine izolovano je dvanaest sojeva faga iz rizosfere obolelih biljaka
lubenice, kako i iz listova lubenice sa simptomima bakteriozne mrljavosti. Karakterizacija
sojeva izvršena je na osnovu spektra domaćina virusa, morfoloških odlika plakova i virusnih
čestica, termalne tačke inaktivacije, parametara umnožavanja faga, kao i RFLP analize.
Rezultati istraživanja pokazali su da svi sojevi faga liziraju 30 od ukupno 32 testirana
soja A. citrulli izolovana u Srbiji. Fagi su ispoljili vosoku specifičnost prema vrsti i nisu lizirali
ostale testirane vrste patogenih i saprofitnih bakterija. Svi sojevi formirali su prozračne
plakove, prečnika 2 mm, nakon 24 h inkubacije. Pomoću transmisionog elektronskog
mikrosopa, utvrđeno je da fagi pripadaju redu Caudovirales, familiji Siphoviridae. Tokom 20
min 90% faga se adsorbuje na površinu bakterijske ćelije, dok se po jednoj inficiranoj ćeliji
oslobodi prosečno 74 ± 5 čestice virusa. Termalna tačka inaktivacije faga iznosila je 66 ili
67ºC. Fagi su ispoljili osetljivost prema hloroformu, bili su stablini pri pH 5-9, ali inaktivirani
nakon 5-10 min izloženosti UV svetlu. RFLP analizom, korišćenjem EcoRI, BsmI and BamHI
enzima nisu utvrđene genetičke razlike među proučavanim sojevima faga.
Ova istraživanja stvaraju osnovu za dalji razvoj inovativnih metoda za kontrolu
bakteriozne mrljavosti lubenice primenom prirodnih mehanizama zaštite.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor",
title = "KARAKTERIZACIJA BAKTERIOFAGA SPECIFIČNIH PREMA Acidovorax citrulli – PROUZROKOVAČU MRLJAVOSTI PLODA LUBENICE",
number = "17"
}

Gašić, K., Obradović, M., Blagojević, N.,& Obradović, A.. (2021). KARAKTERIZACIJA BAKTERIOFAGA SPECIFIČNIH PREMA Acidovorax citrulli – PROUZROKOVAČU MRLJAVOSTI PLODA LUBENICE. in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor
Društvo za zaštitu bilja.(17).

Gašić K, Obradović M, Blagojević N, Obradović A. KARAKTERIZACIJA BAKTERIOFAGA SPECIFIČNIH PREMA Acidovorax citrulli – PROUZROKOVAČU MRLJAVOSTI PLODA LUBENICE. in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor. 2021;(17)..

Gašić, Katarina, Obradović, Mina, Blagojević, Nevena, Obradović, Aleksa, "KARAKTERIZACIJA BAKTERIOFAGA SPECIFIČNIH PREMA Acidovorax citrulli – PROUZROKOVAČU MRLJAVOSTI PLODA LUBENICE" in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor, no. 17 (2021).

TY  - CONF
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1277
AB  - Bakteriozna mrljavost plodova lubenice, prouzrokovana fitopatogenom bakterijom
Acidovorax citrulli, predstavlja ozbiljan problem u proizvodnji lubenice još od osamdesetih
godina prošlog veka. Bakterija je detektovana po prvi put na Floridi, a značajniji gubici u
proizvodnji beleže se u oblasti severne Amerike počev od 1987. godine. Patogen se ubrzo
proširio širom sveta, uglavnom putem zaraženog semena i sadnog materijala. Različite
korovske i gajene vrste familije Cucurbitaceae mogu biti domaćini ove bakterije, ali lubenica i
dinja spadaju u najosetljivije. U uslovima pogodnim za razvoj infekcije, gubici u proizvodnji
mogu biti potpuni. Uprkos pretpostavci da su jedini domaćini ove bakterije vrste familije
tikava, grupa izraelskih autora izolovala je A. citrulli iz obolelih biljaka paradajza i plavog
patlidžana tokom 2012. i 2014. godine, dok je 2020. godine bolest detektovana i u rasadu
paradajza u Grčkoj. Ovi izveštaji ukazuju na to da A. citrulli potencijalno može ugroziti
proizvodnju i drugih biljnih vrsta, izvan familije Cucurbitaceae. Prema podacima svetske
literature, sojevi A. citrulli se mogu svrstati u dve biohemijsko-fiziološki i genetički udaljene
grupe, pa tako grupi I pripadaju sojevi izolovani iz različitih vrsta familije Cucurbitaceae, dok
grupi II pripadaju uglavnom sojevi izolovani iz lubenice.
U Srbiji A. citrulli predstavlja karantinsku vrstu, koja je po prvi put detektovana 2014.
godine i od tada se vrši redovno praćenje pojave patogena. U proizvodnji lubenice, na
pojedinim poljima gde je utvrđena pojava bolesti, gubici su iznosili preko 90% ukupnog
prinosa. Tokom osmogodišnjeg perioda, izolovano je preko 50 sojeva A. citrulli u Srbiji.
Proučene su biohemijsko-fiziološke osobine, rezistentnost prema baktericidima, krug
domaćina, stepen virulentnosti prema različitim sortama lubenice, kao i genetičke
karakteristike sojeva. Na osnovu rezultata naših istraživanja, utvrđeno je da proučavani sojevi
imaju homogenu strukturu populacije i pripadaju grupi II. Ovaj podatak do nedavno je
ukazivao da će lubenica i dalje biti najugroženiji domaćin u nas. Međutim, najnoviji podaci
ukazuju da sojevi ove grupe mogu prouzrokovati bolest i na drugim vrstama, kao što su plavi
patlidžan i paradajz. Stoga se nameće potreba za dodatnom opreznošću, primenom
karantinskih i fitosanitarnih mera, kao i intenzivnijem izučavanju epidemioloških
karakteristika ove bakterije.
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021
T1  - Acidovorax citrulli - NOVA SAZNANJA O POPULACIJI POREKLOM IZ SRBIJE I POTENCIJALNO ŠIREM KRUGU DOMAĆINA
SP  - 16
ER  - 

@conference{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Anđelka and Kuzmanović, Nemanja and Ivanović, Milan and Obradović, Aleksa",
year = "2021",
abstract = "Bakteriozna mrljavost plodova lubenice, prouzrokovana fitopatogenom bakterijom
Acidovorax citrulli, predstavlja ozbiljan problem u proizvodnji lubenice još od osamdesetih
godina prošlog veka. Bakterija je detektovana po prvi put na Floridi, a značajniji gubici u
proizvodnji beleže se u oblasti severne Amerike počev od 1987. godine. Patogen se ubrzo
proširio širom sveta, uglavnom putem zaraženog semena i sadnog materijala. Različite
korovske i gajene vrste familije Cucurbitaceae mogu biti domaćini ove bakterije, ali lubenica i
dinja spadaju u najosetljivije. U uslovima pogodnim za razvoj infekcije, gubici u proizvodnji
mogu biti potpuni. Uprkos pretpostavci da su jedini domaćini ove bakterije vrste familije
tikava, grupa izraelskih autora izolovala je A. citrulli iz obolelih biljaka paradajza i plavog
patlidžana tokom 2012. i 2014. godine, dok je 2020. godine bolest detektovana i u rasadu
paradajza u Grčkoj. Ovi izveštaji ukazuju na to da A. citrulli potencijalno može ugroziti
proizvodnju i drugih biljnih vrsta, izvan familije Cucurbitaceae. Prema podacima svetske
literature, sojevi A. citrulli se mogu svrstati u dve biohemijsko-fiziološki i genetički udaljene
grupe, pa tako grupi I pripadaju sojevi izolovani iz različitih vrsta familije Cucurbitaceae, dok
grupi II pripadaju uglavnom sojevi izolovani iz lubenice.
U Srbiji A. citrulli predstavlja karantinsku vrstu, koja je po prvi put detektovana 2014.
godine i od tada se vrši redovno praćenje pojave patogena. U proizvodnji lubenice, na
pojedinim poljima gde je utvrđena pojava bolesti, gubici su iznosili preko 90% ukupnog
prinosa. Tokom osmogodišnjeg perioda, izolovano je preko 50 sojeva A. citrulli u Srbiji.
Proučene su biohemijsko-fiziološke osobine, rezistentnost prema baktericidima, krug
domaćina, stepen virulentnosti prema različitim sortama lubenice, kao i genetičke
karakteristike sojeva. Na osnovu rezultata naših istraživanja, utvrđeno je da proučavani sojevi
imaju homogenu strukturu populacije i pripadaju grupi II. Ovaj podatak do nedavno je
ukazivao da će lubenica i dalje biti najugroženiji domaćin u nas. Međutim, najnoviji podaci
ukazuju da sojevi ove grupe mogu prouzrokovati bolest i na drugim vrstama, kao što su plavi
patlidžan i paradajz. Stoga se nameće potreba za dodatnom opreznošću, primenom
karantinskih i fitosanitarnih mera, kao i intenzivnijem izučavanju epidemioloških
karakteristika ove bakterije.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021",
title = "Acidovorax citrulli - NOVA SAZNANJA O POPULACIJI POREKLOM IZ SRBIJE I POTENCIJALNO ŠIREM KRUGU DOMAĆINA",
pages = "16"
}

Zlatković, N., Gašić, K., Prokić, A., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2021). Acidovorax citrulli - NOVA SAZNANJA O POPULACIJI POREKLOM IZ SRBIJE I POTENCIJALNO ŠIREM KRUGU DOMAĆINA. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021
Društvo za zaštitu bilja., 16.

Zlatković N, Gašić K, Prokić A, Kuzmanović N, Ivanović M, Obradović A. Acidovorax citrulli - NOVA SAZNANJA O POPULACIJI POREKLOM IZ SRBIJE I POTENCIJALNO ŠIREM KRUGU DOMAĆINA. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021. 2021;:16..

Zlatković, Nevena, Gašić, Katarina, Prokić, Anđelka, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "Acidovorax citrulli - NOVA SAZNANJA O POPULACIJI POREKLOM IZ SRBIJE I POTENCIJALNO ŠIREM KRUGU DOMAĆINA" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021 (2021):16.

TY  - CONF
AU  - Popović, Tamara
AU  - Menković, Jelena
AU  - Prokić, Anđelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1278
AB  - Pseudomonas syringae pv. syringae (Pss) prouzrokovač bakterioznog raka je značajan
patogen koštičavih voćaka i badema širom svijeta. U Crnoj Gori Pss kao patogen koštičavih
voćaka i badema nije detaljnije proučen. Tokom dvogodišnjeg nadzora (2017-2018),
prikupljeni su simptomatični uzorci raznih organa koštičavih voćaka i badema, iz različitih
lokaliteta širom Crne Gore. Na oboljelim voćkama uočavali su se različiti simptomi: nekroza i
rak-rane na granama, često praćene isticanjem smole; sušenje grančica i grana; pjegavost
listova; nekroza pupoljaka i lisnih drški. Iz oboljelog tkiva, izolovano je 29 sojeva
fitopatogenih bakterija koji su proučeni primjenom klasičnih i specifičnih diferencijalnih
bakterioloških testova, kao i molekularnih metoda. Na podlozi od hranljivog agara (HA)
proučavani sojevi formirali su ispupčene, sjajne, sivobjeličaste krupne kolonije. Patogenost
proučavanih sojeva potvrđena je reprodukcijom simptoma na listovima, plodovima i
21
mladarima odgovarajućih biljaka domaćina. Proučavani sojevi stvaraju zeleni fluorescentni
pigment na Kingovoj podlozi B. Svi proučavani sojevi su Gram negativni, stvaraju katalazu, ali
ne i oksidazu, imaju oksidativan metabolizam glukoze i prouzrokuju hipersenzitivnu reakciju
lista duvana i muškatle. Uočena je varijabilnost u pogledu stvaranja levana i vitalnosti na
hranljivoj podlozi obogaćenoj saharozom (NAS), kao i sposobnosti stvaranja čestica leda.
Rezultati GATTa testova nisu bili dovoljni i pouzdani za diferencijaciju P. syringae sojeva do ili
ispod nivoa patogenog varijeteta. Kod 25 proučavanih sojeva detektovan je syrB gen koji
kodira sintezu sringomicina. Rezultati Rep-PCR primjenom BOX prajmera, pokazali su visok
genetički diverzitet među proučavanim sojevima. Analiza multilokusnih sekvenci (MLSA) je
izvedena za 28 proučavanih sojeva, pri čemu su umnožene nukleotidne sekvence 4
konstitutivna gena: gapA, gltA, gyrB i rpoD. Rezultati MLSA pokazuju da 27 proučavanih
sojeva pripada genomskoj vrsti 1, P. syringae sensu stricto, raspoređenih u filogrupi 2.
Međutim, sojevi iz iste filogrupe 2, nisu grupisani u jedan monofiletski klaster. Izuzetak je
predstavljao soj K6 izolovan iz kajsije, koji pripada genomskoj vrsti 2, filogrupi 3. Kod četiri
soja kod kojih nije detektovan gen za sintezu siringomicina, analiziran je 16S rRNK gen.
Poređenjem dobijenih sekvenci sa sekvencama u NCBI bazi, utvrđeno je da ti sojevi ipak
pripadaju vrsti Pss. Na osnovu dobijenih rezultata, 28 sojeva je identifikovano kao Pss.
Izuzetak je soj K6 čija taksonomska kategorija još uvek nije utvrđena.
PB  - Društvo za zaštitu bilja
C3  - XVI Savetovanje o zaštiti bilja, 22-25. februar 2021. godine, Zlatibor, Srbija. Zbornik rezimea
T1  - POPULACIJA Pseudomonas syringae pv. syringae - PATOGENA KOŠTIČAVIH VOĆAKA I BADEMA U CRNOJ GORI
EP  - 21
SP  - 20
ER  - 

@conference{
author = "Popović, Tamara and Menković, Jelena and Prokić, Anđelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2021",
abstract = "Pseudomonas syringae pv. syringae (Pss) prouzrokovač bakterioznog raka je značajan
patogen koštičavih voćaka i badema širom svijeta. U Crnoj Gori Pss kao patogen koštičavih
voćaka i badema nije detaljnije proučen. Tokom dvogodišnjeg nadzora (2017-2018),
prikupljeni su simptomatični uzorci raznih organa koštičavih voćaka i badema, iz različitih
lokaliteta širom Crne Gore. Na oboljelim voćkama uočavali su se različiti simptomi: nekroza i
rak-rane na granama, često praćene isticanjem smole; sušenje grančica i grana; pjegavost
listova; nekroza pupoljaka i lisnih drški. Iz oboljelog tkiva, izolovano je 29 sojeva
fitopatogenih bakterija koji su proučeni primjenom klasičnih i specifičnih diferencijalnih
bakterioloških testova, kao i molekularnih metoda. Na podlozi od hranljivog agara (HA)
proučavani sojevi formirali su ispupčene, sjajne, sivobjeličaste krupne kolonije. Patogenost
proučavanih sojeva potvrđena je reprodukcijom simptoma na listovima, plodovima i
21
mladarima odgovarajućih biljaka domaćina. Proučavani sojevi stvaraju zeleni fluorescentni
pigment na Kingovoj podlozi B. Svi proučavani sojevi su Gram negativni, stvaraju katalazu, ali
ne i oksidazu, imaju oksidativan metabolizam glukoze i prouzrokuju hipersenzitivnu reakciju
lista duvana i muškatle. Uočena je varijabilnost u pogledu stvaranja levana i vitalnosti na
hranljivoj podlozi obogaćenoj saharozom (NAS), kao i sposobnosti stvaranja čestica leda.
Rezultati GATTa testova nisu bili dovoljni i pouzdani za diferencijaciju P. syringae sojeva do ili
ispod nivoa patogenog varijeteta. Kod 25 proučavanih sojeva detektovan je syrB gen koji
kodira sintezu sringomicina. Rezultati Rep-PCR primjenom BOX prajmera, pokazali su visok
genetički diverzitet među proučavanim sojevima. Analiza multilokusnih sekvenci (MLSA) je
izvedena za 28 proučavanih sojeva, pri čemu su umnožene nukleotidne sekvence 4
konstitutivna gena: gapA, gltA, gyrB i rpoD. Rezultati MLSA pokazuju da 27 proučavanih
sojeva pripada genomskoj vrsti 1, P. syringae sensu stricto, raspoređenih u filogrupi 2.
Međutim, sojevi iz iste filogrupe 2, nisu grupisani u jedan monofiletski klaster. Izuzetak je
predstavljao soj K6 izolovan iz kajsije, koji pripada genomskoj vrsti 2, filogrupi 3. Kod četiri
soja kod kojih nije detektovan gen za sintezu siringomicina, analiziran je 16S rRNK gen.
Poređenjem dobijenih sekvenci sa sekvencama u NCBI bazi, utvrđeno je da ti sojevi ipak
pripadaju vrsti Pss. Na osnovu dobijenih rezultata, 28 sojeva je identifikovano kao Pss.
Izuzetak je soj K6 čija taksonomska kategorija još uvek nije utvrđena.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI Savetovanje o zaštiti bilja, 22-25. februar 2021. godine, Zlatibor, Srbija. Zbornik rezimea",
title = "POPULACIJA Pseudomonas syringae pv. syringae - PATOGENA KOŠTIČAVIH VOĆAKA I BADEMA U CRNOJ GORI",
pages = "21-20"
}

Popović, T., Menković, J., Prokić, A., Zlatković, N.,& Obradović, A.. (2021). POPULACIJA Pseudomonas syringae pv. syringae - PATOGENA KOŠTIČAVIH VOĆAKA I BADEMA U CRNOJ GORI. in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021. godine, Zlatibor, Srbija. Zbornik rezimea
Društvo za zaštitu bilja., 20-21.

Popović T, Menković J, Prokić A, Zlatković N, Obradović A. POPULACIJA Pseudomonas syringae pv. syringae - PATOGENA KOŠTIČAVIH VOĆAKA I BADEMA U CRNOJ GORI. in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021. godine, Zlatibor, Srbija. Zbornik rezimea. 2021;:20-21..

Popović, Tamara, Menković, Jelena, Prokić, Anđelka, Zlatković, Nevena, Obradović, Aleksa, "POPULACIJA Pseudomonas syringae pv. syringae - PATOGENA KOŠTIČAVIH VOĆAKA I BADEMA U CRNOJ GORI" in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021. godine, Zlatibor, Srbija. Zbornik rezimea (2021):20-21.

TY  - CONF
AU  - Ivanović, Milan M
AU  - Šević, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Zlatković, Nevena
AU  - Menković, Jelena
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1275
AB  - biljaka u vidu guste sadnje, korišćenja osetljivih podloga i novih, osetljivih sorti, doprinose
češćoj i jačoj pojavi bolesti biljaka. U tom pogledu bakteriozna obolenja ne predstavljaju
izuzetak. U uslovima pogodnim za razvoj i širenje bolesti, fitopatogene bakterije za kratko
vreme mogu pričiniti ogromne štete.
Ukrupnjavanje poseda i velike površine pod jednom biljnom vrstom, osim što stvaraju
uslove za brzo širenje patogena, često smanjuju mogućnost i efekte primene preventivnih
agrotehničkih, mehaničkih, fizičkih i bioloških mera zaštite. U takvoj situaciji proizvođači se
uglavnom odlučuju za mere intervencije odnosno zaštitu hemijskim sredstvima. Jedan od
načina kontrole bakterioznih oboljenja je primena preparata na bazi bakra. Međutim,
ograničena primena tokom mirovanja ili do određene fenofaze razvoja biljaka, fitotoksičnost
jona bakra usled niskih temperatura i pojava rezistentnosti fitopatogenih bakterija dovode u
pitanje efikasnost bakarnih preparata. Stoga se u pojedinim državama za suzbijanje
fitopatogenih bakterija koriste i antibiotici. To su proizvodi biološke sinteze aktinomiceta,
bakterija i gljiva, ili hemijski sintetisane materije koji deluju inhibitorno na mikroorganizme.
Ipak, upotrebu antibiotika u zaštiti bilja prate mnoge kontroverze, ali i zablude.
Neke od osnovanih bojazni vezane za upotrebu antibiotika u zaštiti bilja su: razvoj
rezistentnih sojeva bakterija, rizik od transfera gena rezistentnosti iz poljoprivredne sredine u
humanu populaciju, uticaj na neciljane mikroorganizme i smanjenje genetičkog diverziteta,
kao i pitanje ostataka antibiotika u biljnim proizvodima i posledice koje mogu nastati zbog
toga.
S druge strane, jedna od najvećih zabluda je da se antibiotici široko primenjuju u zaštiti
bilja od bakterioznih obolenja. Iskustva iz SAD zapravo pokazuju da se antibiotici u zaštit bilja
u najvećoj meri koriste u kontroli jednog obolenja: bakteriozne plamenjače jabučastih voćaka.
Količina upotrebljenih antibiotika u zaštiti bilja u SAD su zanemarljivo male, svega 0,26%, dok
se preostali deo upotrebi u uzgoju životinja. U našoj zemlji nije dozvoljena upotreba
antibiotika u zaštiti bilja. Ipak, situacija na terenu ukazuje na njihovo nedozvoljeno korišćenje
u pojedinim slučajevima. Dodatnu opasnost predstavlja aktivna materija koja se tom prilikom
koristi, a to je streptomicin sulfat koji je zapravo registrovan za primenu u veterinarskoj
medicini, a ne kao formulacija prilagođena zaštiti bilja. Takva praksa stvara uslove za
ostvarenje navedenih bojazni, a takođe može ugroziti poverenje domaćih potrošača kao i
plasman poljoprivrednih proizvoda na inostrano tržište. Pojedine evropske zemlje poput Austrije, Švajcarske i Nemačke regulisale su upotrebu antibiotika u zaštiti od bakteriozne
plamenjače izdavanjem specijalnih dozvola od strane nadležnih institucija u godinama kada se
proceni da postoji jak infekcioni pritisak i povoljni uslovi za razvoj bolesti. Možda je model
strogo kontrolisane upotrebe onaj koji bi zadovoljio sve strane: proizvođače, potrošače i
zakonodavca, a sve u cilju smanjenja neodgovorne upotrebe antibiotika u zaštiti bilja.
PB  - Društvo za zaštitu bilja
C3  - XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor
T1  - PRIMENA ANTIBIOTIKA U ZAŠTITI BILJA – ZA I PROTIV
EP  - 30
SP  - 29
ER  - 

@conference{
author = "Ivanović, Milan M and Šević, Milan and Prokić, Anđelka and Gašić, Katarina and Zlatković, Nevena and Menković, Jelena and Obradović, Aleksa",
year = "2021",
abstract = "biljaka u vidu guste sadnje, korišćenja osetljivih podloga i novih, osetljivih sorti, doprinose
češćoj i jačoj pojavi bolesti biljaka. U tom pogledu bakteriozna obolenja ne predstavljaju
izuzetak. U uslovima pogodnim za razvoj i širenje bolesti, fitopatogene bakterije za kratko
vreme mogu pričiniti ogromne štete.
Ukrupnjavanje poseda i velike površine pod jednom biljnom vrstom, osim što stvaraju
uslove za brzo širenje patogena, često smanjuju mogućnost i efekte primene preventivnih
agrotehničkih, mehaničkih, fizičkih i bioloških mera zaštite. U takvoj situaciji proizvođači se
uglavnom odlučuju za mere intervencije odnosno zaštitu hemijskim sredstvima. Jedan od
načina kontrole bakterioznih oboljenja je primena preparata na bazi bakra. Međutim,
ograničena primena tokom mirovanja ili do određene fenofaze razvoja biljaka, fitotoksičnost
jona bakra usled niskih temperatura i pojava rezistentnosti fitopatogenih bakterija dovode u
pitanje efikasnost bakarnih preparata. Stoga se u pojedinim državama za suzbijanje
fitopatogenih bakterija koriste i antibiotici. To su proizvodi biološke sinteze aktinomiceta,
bakterija i gljiva, ili hemijski sintetisane materije koji deluju inhibitorno na mikroorganizme.
Ipak, upotrebu antibiotika u zaštiti bilja prate mnoge kontroverze, ali i zablude.
Neke od osnovanih bojazni vezane za upotrebu antibiotika u zaštiti bilja su: razvoj
rezistentnih sojeva bakterija, rizik od transfera gena rezistentnosti iz poljoprivredne sredine u
humanu populaciju, uticaj na neciljane mikroorganizme i smanjenje genetičkog diverziteta,
kao i pitanje ostataka antibiotika u biljnim proizvodima i posledice koje mogu nastati zbog
toga.
S druge strane, jedna od najvećih zabluda je da se antibiotici široko primenjuju u zaštiti
bilja od bakterioznih obolenja. Iskustva iz SAD zapravo pokazuju da se antibiotici u zaštit bilja
u najvećoj meri koriste u kontroli jednog obolenja: bakteriozne plamenjače jabučastih voćaka.
Količina upotrebljenih antibiotika u zaštiti bilja u SAD su zanemarljivo male, svega 0,26%, dok
se preostali deo upotrebi u uzgoju životinja. U našoj zemlji nije dozvoljena upotreba
antibiotika u zaštiti bilja. Ipak, situacija na terenu ukazuje na njihovo nedozvoljeno korišćenje
u pojedinim slučajevima. Dodatnu opasnost predstavlja aktivna materija koja se tom prilikom
koristi, a to je streptomicin sulfat koji je zapravo registrovan za primenu u veterinarskoj
medicini, a ne kao formulacija prilagođena zaštiti bilja. Takva praksa stvara uslove za
ostvarenje navedenih bojazni, a takođe može ugroziti poverenje domaćih potrošača kao i
plasman poljoprivrednih proizvoda na inostrano tržište. Pojedine evropske zemlje poput Austrije, Švajcarske i Nemačke regulisale su upotrebu antibiotika u zaštiti od bakteriozne
plamenjače izdavanjem specijalnih dozvola od strane nadležnih institucija u godinama kada se
proceni da postoji jak infekcioni pritisak i povoljni uslovi za razvoj bolesti. Možda je model
strogo kontrolisane upotrebe onaj koji bi zadovoljio sve strane: proizvođače, potrošače i
zakonodavca, a sve u cilju smanjenja neodgovorne upotrebe antibiotika u zaštiti bilja.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor",
title = "PRIMENA ANTIBIOTIKA U ZAŠTITI BILJA – ZA I PROTIV",
pages = "30-29"
}

Ivanović, M. M., Šević, M., Prokić, A., Gašić, K., Zlatković, N., Menković, J.,& Obradović, A.. (2021). PRIMENA ANTIBIOTIKA U ZAŠTITI BILJA – ZA I PROTIV. in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor
Društvo za zaštitu bilja., 29-30.

Ivanović MM, Šević M, Prokić A, Gašić K, Zlatković N, Menković J, Obradović A. PRIMENA ANTIBIOTIKA U ZAŠTITI BILJA – ZA I PROTIV. in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor. 2021;:29-30..

Ivanović, Milan M, Šević, Milan, Prokić, Anđelka, Gašić, Katarina, Zlatković, Nevena, Menković, Jelena, Obradović, Aleksa, "PRIMENA ANTIBIOTIKA U ZAŠTITI BILJA – ZA I PROTIV" in XVI Savetovanje o zaštiti bilja, 22-25. februar 2021., Zlatibor (2021):29-30.

TY  - CONF
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Živković, Svetlana
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1276
AB  - Pojava bakteriozne mrljavosti plodova lubenice uočena je šezdesetih godina prošlog
veka na Floridi, ali pažnju istraživača privukla je tek nakon dvadeset godina, kada su usled
iznenadne pojave epidemije nastali ogromni gubici u proizvodnji lubenice u nekoliko saveznih
američkih država. Na teritoriji naše zemlje prouzrokovač ovog oboljenja, karantinska vrsta A.
citrulli, po prvi put dospeva putem zaraženog semena 2014. godine.
Nakon ovog nalaza, patogen je sporadično detektovan na različitim lokalitetima i u
narednim sezonama. S obzirom na gubitke u proizvodnji koji mogu nastati usled pojave ovog
oboljenja, karantinskog statusa koji i dalje ima na teritoriji naše zemlje, nastavljena su dalja
proučavanja izolovanih sojeva. Pored realizovanih istraživanja o diverzitetu sojeva na
biohemijsko-fiziološkom nivou, u okviru ove studije proučavane su genetičke osobine
izolovanih sojeva.
Na osnovu rezultata prethodnih istraživanja, sojevi A. citrulli se mogu razdvojiti u dve
genetički različite grupe. Ova podela se dovodi u vezu sa krugom domaćina, pa tako grupi I
pripadaju sojevi koji su izolovani i ispoljavaju visoku virulentnost prema različitim
domaćinima familije Cucurbitaceae, dok su sojevi II grupe izolovani iz lubenice, prema kojoj
su naročito agresivni. Međutim, intenzivna proučavanja sojeva A. citrulli u svetu poslednjih
nekoliko godina, pokazala su da se ne može postaviti jasna granica među sojevima samo na
osnovu domaćina i da su rezultati u slučaju prirodne i veštački izazvane infekcije izrazito
varijabilni. Tako je pokazano da u uslovima veštačke inokulacije nije preterano izražena
specifičnost sojeva prema domaćinu, za razliku od infekcije nastale prirodnim putem u polju.
Stoga je u okviru naše studije proučena genetička pripadnost različitim grupama 41 soja A.
citrulli poreklom iz Srbije, izolovanih u periodu 2014-2018. godine. Izvedena je PCR reakcija
prema protokolu Živanović and Walcott (2016), gde se primenom posebno dizajniranih
prajmera, sojevi razvrstavaju u navedene dve grupe. Takođe, u cilju potvrde dobijenih
rezultata, primenjen je i duplex PCR, prema protokolu Zhao et al. (2020). Rezultati testiranja
su pokazali da sojevi izolovani u Srbiji predstavljaju homogenu populaciju i pripradaju II grupi
sojeva patogena lubenice. Ovakav rezultat se mogao očekivati imajući u vidu da su svi
proučavani sojevi izolovani iz biljaka lubenice.
Činjenica da proučavani sojevi pripadaju grupi II ukazuje da će lubenica i dalje biti
najugroženiji domaćin ove bakterije u nas, dok su manje verovatne štete u proizvodnji drugih
vrsta familije Cucurbitaceae.
PB  - Društvo za zaštitu bilja
C3  - XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine
T1  - HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE
EP  - 63
SP  - 62
ER  - 

@conference{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Anđelka and Kuzmanović, Nemanja and Ivanović, Milan and Živković, Svetlana and Obradović, Aleksa",
year = "2021",
abstract = "Pojava bakteriozne mrljavosti plodova lubenice uočena je šezdesetih godina prošlog
veka na Floridi, ali pažnju istraživača privukla je tek nakon dvadeset godina, kada su usled
iznenadne pojave epidemije nastali ogromni gubici u proizvodnji lubenice u nekoliko saveznih
američkih država. Na teritoriji naše zemlje prouzrokovač ovog oboljenja, karantinska vrsta A.
citrulli, po prvi put dospeva putem zaraženog semena 2014. godine.
Nakon ovog nalaza, patogen je sporadično detektovan na različitim lokalitetima i u
narednim sezonama. S obzirom na gubitke u proizvodnji koji mogu nastati usled pojave ovog
oboljenja, karantinskog statusa koji i dalje ima na teritoriji naše zemlje, nastavljena su dalja
proučavanja izolovanih sojeva. Pored realizovanih istraživanja o diverzitetu sojeva na
biohemijsko-fiziološkom nivou, u okviru ove studije proučavane su genetičke osobine
izolovanih sojeva.
Na osnovu rezultata prethodnih istraživanja, sojevi A. citrulli se mogu razdvojiti u dve
genetički različite grupe. Ova podela se dovodi u vezu sa krugom domaćina, pa tako grupi I
pripadaju sojevi koji su izolovani i ispoljavaju visoku virulentnost prema različitim
domaćinima familije Cucurbitaceae, dok su sojevi II grupe izolovani iz lubenice, prema kojoj
su naročito agresivni. Međutim, intenzivna proučavanja sojeva A. citrulli u svetu poslednjih
nekoliko godina, pokazala su da se ne može postaviti jasna granica među sojevima samo na
osnovu domaćina i da su rezultati u slučaju prirodne i veštački izazvane infekcije izrazito
varijabilni. Tako je pokazano da u uslovima veštačke inokulacije nije preterano izražena
specifičnost sojeva prema domaćinu, za razliku od infekcije nastale prirodnim putem u polju.
Stoga je u okviru naše studije proučena genetička pripadnost različitim grupama 41 soja A.
citrulli poreklom iz Srbije, izolovanih u periodu 2014-2018. godine. Izvedena je PCR reakcija
prema protokolu Živanović and Walcott (2016), gde se primenom posebno dizajniranih
prajmera, sojevi razvrstavaju u navedene dve grupe. Takođe, u cilju potvrde dobijenih
rezultata, primenjen je i duplex PCR, prema protokolu Zhao et al. (2020). Rezultati testiranja
su pokazali da sojevi izolovani u Srbiji predstavljaju homogenu populaciju i pripradaju II grupi
sojeva patogena lubenice. Ovakav rezultat se mogao očekivati imajući u vidu da su svi
proučavani sojevi izolovani iz biljaka lubenice.
Činjenica da proučavani sojevi pripadaju grupi II ukazuje da će lubenica i dalje biti
najugroženiji domaćin ove bakterije u nas, dok su manje verovatne štete u proizvodnji drugih
vrsta familije Cucurbitaceae.",
publisher = "Društvo za zaštitu bilja",
journal = "XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine",
title = "HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE",
pages = "63-62"
}

Zlatković, N., Gašić, K., Prokić, A., Kuzmanović, N., Ivanović, M., Živković, S.,& Obradović, A.. (2021). HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE. in XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine
Društvo za zaštitu bilja., 62-63.

Zlatković N, Gašić K, Prokić A, Kuzmanović N, Ivanović M, Živković S, Obradović A. HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE. in XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine. 2021;:62-63..

Zlatković, Nevena, Gašić, Katarina, Prokić, Anđelka, Kuzmanović, Nemanja, Ivanović, Milan, Živković, Svetlana, Obradović, Aleksa, "HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE" in XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine (2021):62-63.

TY  - CONF
AU  - Kovacs, Tamas
AU  - Bali, Dominika
AU  - Solti-Hodovan, Agnes
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Stefani, Emilio
AU  - Altin, Irem
AU  - Gopalakrishnan, Chelappan
AU  - Fieseler, Lars
AU  - Ravasz, Szabolcs
AU  - Rakhely, Gabor
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1271
AB  - Bacterial plant diseases can cause significant economic losses worldwide. There is an urgent need for the development
and application of sustainable biocontrol agents against plant pathogenic bacteria. Bacteriophages are promising antimicrobial
agents, as it was demonstrated for five phytopathogenic bacteria. Phages infecting Acidovorax citrulli, causing
seedling blight and bacterial fruit blotch of cucurbits, were isolated and characterized. Lytic life cycle and some of the biological
characteristics of investigated phage strains indicated their potential in the control of watermelon fruit blotch. Xanthomonas
euvesicatoria causes bacterial spot disease of pepper and tomato. Phage KΦ1 was isolated from the rhizosphere
of pepper plants showing symptoms of bacterial spot. Foliar applications of the unformulated KΦ1 phage suspension
effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. Xanthomonas
oryzae pv. oryzae (Xoo) is the most devastating agent of rice, causing its bacterial leaf blight (BLB). We isolated and characterized
ten OP2-like bacteriophages. Laboratory efficacy trials strengthened the hypothesis that these bacteriophages
can control BLB effectively. A bacteriophage cocktail protected rice plants against Xoo successfully during a field trial.
Xanthomonas arboricola pv. juglandis (Xaj) causes walnut blight disease. Bacteriophages against Xaj were isolated from
Italian and Hungarian samples and characterized. Two phages were tested individually for their ability to suppress symptoms
in walnut plantlets following bacterial inoculation. A significant reduction in the degree of symptoms was observed
following phage application when compared to the control. We formulated a cocktail containing six bacteriophages and
implemented field trials by spraying the phage suspension on different Juglans regia varieties. The applied bacteriophage
cocktail protected walnut trees against Xaj effectively, albeit the efficacy was different depending on which variety was
treated. Erwinia amylovora causes fire blight disease of Rosaceae plants. 125 bacteriophages were isolated from soil, and
aerial samples were taken in Hungary, Romania, and Spain, from which 55 showed lytic plaque morphology. Based on
host range analysis conducted on 101 bacterium strains, 27 phages were subject of detailed characterization. The applied
bacteriophage cocktail carefully formulated protected apple trees against E. amylovora during two field trials. A special
permit was issued for marketing the Erwiphage bacteriophage-based biopesticide in 2012. This product was the first bacteriophage-
based biocontrol agent against E. amylovora and the second one among all bacteriophages-based biopesticide
that was available on the market worldwide. Our results support that bacteriophages are potent biocontrol agents against
phytopathogenic bacteria.
PB  - University of Pecs
C3  - 6th Central European Forum for Microbiology, 14-16, October 2021, Kecskemét, Hungary
T1  - BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA
ER  - 

@conference{
author = "Kovacs, Tamas and Bali, Dominika and Solti-Hodovan, Agnes and Obradović, Aleksa and Gašić, Katarina and Stefani, Emilio and Altin, Irem and Gopalakrishnan, Chelappan and Fieseler, Lars and Ravasz, Szabolcs and Rakhely, Gabor",
year = "2021",
abstract = "Bacterial plant diseases can cause significant economic losses worldwide. There is an urgent need for the development
and application of sustainable biocontrol agents against plant pathogenic bacteria. Bacteriophages are promising antimicrobial
agents, as it was demonstrated for five phytopathogenic bacteria. Phages infecting Acidovorax citrulli, causing
seedling blight and bacterial fruit blotch of cucurbits, were isolated and characterized. Lytic life cycle and some of the biological
characteristics of investigated phage strains indicated their potential in the control of watermelon fruit blotch. Xanthomonas
euvesicatoria causes bacterial spot disease of pepper and tomato. Phage KΦ1 was isolated from the rhizosphere
of pepper plants showing symptoms of bacterial spot. Foliar applications of the unformulated KΦ1 phage suspension
effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. Xanthomonas
oryzae pv. oryzae (Xoo) is the most devastating agent of rice, causing its bacterial leaf blight (BLB). We isolated and characterized
ten OP2-like bacteriophages. Laboratory efficacy trials strengthened the hypothesis that these bacteriophages
can control BLB effectively. A bacteriophage cocktail protected rice plants against Xoo successfully during a field trial.
Xanthomonas arboricola pv. juglandis (Xaj) causes walnut blight disease. Bacteriophages against Xaj were isolated from
Italian and Hungarian samples and characterized. Two phages were tested individually for their ability to suppress symptoms
in walnut plantlets following bacterial inoculation. A significant reduction in the degree of symptoms was observed
following phage application when compared to the control. We formulated a cocktail containing six bacteriophages and
implemented field trials by spraying the phage suspension on different Juglans regia varieties. The applied bacteriophage
cocktail protected walnut trees against Xaj effectively, albeit the efficacy was different depending on which variety was
treated. Erwinia amylovora causes fire blight disease of Rosaceae plants. 125 bacteriophages were isolated from soil, and
aerial samples were taken in Hungary, Romania, and Spain, from which 55 showed lytic plaque morphology. Based on
host range analysis conducted on 101 bacterium strains, 27 phages were subject of detailed characterization. The applied
bacteriophage cocktail carefully formulated protected apple trees against E. amylovora during two field trials. A special
permit was issued for marketing the Erwiphage bacteriophage-based biopesticide in 2012. This product was the first bacteriophage-
based biocontrol agent against E. amylovora and the second one among all bacteriophages-based biopesticide
that was available on the market worldwide. Our results support that bacteriophages are potent biocontrol agents against
phytopathogenic bacteria.",
publisher = "University of Pecs",
journal = "6th Central European Forum for Microbiology, 14-16, October 2021, Kecskemét, Hungary",
title = "BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA"
}

Kovacs, T., Bali, D., Solti-Hodovan, A., Obradović, A., Gašić, K., Stefani, E., Altin, I., Gopalakrishnan, C., Fieseler, L., Ravasz, S.,& Rakhely, G.. (2021). BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA. in 6th Central European Forum for Microbiology, 14-16, October 2021, Kecskemét, Hungary
University of Pecs..

Kovacs T, Bali D, Solti-Hodovan A, Obradović A, Gašić K, Stefani E, Altin I, Gopalakrishnan C, Fieseler L, Ravasz S, Rakhely G. BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA. in 6th Central European Forum for Microbiology, 14-16, October 2021, Kecskemét, Hungary. 2021;..

Kovacs, Tamas, Bali, Dominika, Solti-Hodovan, Agnes, Obradović, Aleksa, Gašić, Katarina, Stefani, Emilio, Altin, Irem, Gopalakrishnan, Chelappan, Fieseler, Lars, Ravasz, Szabolcs, Rakhely, Gabor, "BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA" in 6th Central European Forum for Microbiology, 14-16, October 2021, Kecskemét, Hungary (2021).

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Menković, Jelena
AU  - Prokić, Anđelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://enauka.gov.rs/handle/123456789/776640
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1185
AB  - In Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial diseases at different geographical locations in Montenegro. From leaf, petiole and fruit lesions, branch or twig cankers, and necrotizing buds, a total of 29 isolates were obtained and subjected to identification based on their morphological, pathogenic, biochemical, and molecular characteristics. Pathogenicity of the isolates was confirmed by reproducing the symptoms on leaves, fruits, and twigs of the corresponding host plants. The biochemical tests indicated that the isolates belong to Pseudomonas syringae. However, isolates’ characterization showed variation in their phenotypic and molecular features. The presence of the syrB gene and ice nucleation activity grouped most of the isolates within pathovar syringae. The results of rep-PCR using the BOX primer revealed high genetic diversity of isolates. Multilocus sequence analysis (MLSA), using four housekeeping genes, showed that 27 isolates belong to the genomic species 1, P. syringae sensu stricto, corresponding to P. syringae phylogroup 2. However, isolates from the same phylogroup 2 did not form a monophyletic group. One strain isolated from apricot was most distinct and similar to members of genomic species 2, phylogroup 3. All tested isolates showed significant levels of resistance to copper sulfate and high level of sensitivity to streptomycin sulfate in vitro.
PB  - Springer
T2  - Journal of Plant Diseases and Protection
T1  - Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro
EP  - 405
IS  - 2
SP  - 391
VL  - 128
DO  - 10.1007/s41348-020-00417-8
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Menković, Jelena and Prokić, Anđelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2021",
abstract = "In Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial diseases at different geographical locations in Montenegro. From leaf, petiole and fruit lesions, branch or twig cankers, and necrotizing buds, a total of 29 isolates were obtained and subjected to identification based on their morphological, pathogenic, biochemical, and molecular characteristics. Pathogenicity of the isolates was confirmed by reproducing the symptoms on leaves, fruits, and twigs of the corresponding host plants. The biochemical tests indicated that the isolates belong to Pseudomonas syringae. However, isolates’ characterization showed variation in their phenotypic and molecular features. The presence of the syrB gene and ice nucleation activity grouped most of the isolates within pathovar syringae. The results of rep-PCR using the BOX primer revealed high genetic diversity of isolates. Multilocus sequence analysis (MLSA), using four housekeeping genes, showed that 27 isolates belong to the genomic species 1, P. syringae sensu stricto, corresponding to P. syringae phylogroup 2. However, isolates from the same phylogroup 2 did not form a monophyletic group. One strain isolated from apricot was most distinct and similar to members of genomic species 2, phylogroup 3. All tested isolates showed significant levels of resistance to copper sulfate and high level of sensitivity to streptomycin sulfate in vitro.",
publisher = "Springer",
journal = "Journal of Plant Diseases and Protection",
title = "Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro",
pages = "405-391",
number = "2",
volume = "128",
doi = "10.1007/s41348-020-00417-8"
}

Popović Milovanović, T., Menković, J., Prokić, A., Zlatković, N.,& Obradović, A.. (2021). Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro. in Journal of Plant Diseases and Protection
Springer., 128(2), 391-405.
https://doi.org/10.1007/s41348-020-00417-8

Popović Milovanović T, Menković J, Prokić A, Zlatković N, Obradović A. Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro. in Journal of Plant Diseases and Protection. 2021;128(2):391-405.
doi:10.1007/s41348-020-00417-8 .

Popović Milovanović, Tatjana, Menković, Jelena, Prokić, Anđelka, Zlatković, Nevena, Obradović, Aleksa, "Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro" in Journal of Plant Diseases and Protection, 128, no. 2 (2021):391-405,
https://doi.org/10.1007/s41348-020-00417-8 . .

Koebnik, R., Gašić, K.,& Obradović, A.. (2021). Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe. in Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe
Belgrade : University, Faculty of Agriculture..

Koebnik R, Gašić K, Obradović A. Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe. in Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe. 2021;..

Koebnik, Ralf, Gašić, Katarina, Obradović, Aleksa, "Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe" in Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe (2021).

Šević, M., Prokić, A., Gašić, K.,& Obradović, A.. (2021). Novi koncept održive strategije kontrole bakteriozne pegavosti paprike integracijom bioloških i hemijskih tretmana. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Šević M, Prokić A, Gašić K, Obradović A. Novi koncept održive strategije kontrole bakteriozne pegavosti paprike integracijom bioloških i hemijskih tretmana. in Institut za zaštitu bilja i životnu sredinu. 2021;..

Šević, Milan, Prokić, Anđelka, Gašić, Katarina, Obradović, Aleksa, "Novi koncept održive strategije kontrole bakteriozne pegavosti paprike integracijom bioloških i hemijskih tretmana" in Institut za zaštitu bilja i životnu sredinu (2021).

TY  - CONF
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
AU  - Gašić, Katarina
AU  - Grahovac, Mila
AU  - Duduk, Bojan
AU  - Milijašević-Marčić, Svetlana
AU  - Ignjatov, Maja
AU  - Šević, Milan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/812
AB  - Istraživanja akademika Živojina Tešića iz 1934. godine, a zatim i prof. Dragoljuba Šutića
u prvoj deceniji posle drugog svetskog rata, na Poljoprivredno-šumarskom, kasnije
Poljoprivrednom fakultetu u Zemunu, mogu se smatrati početkom proučavanja fitopatogenih
bakterija (FPB) u Srbiji. Od tada do danas istraživači su prošli mukotrpan put tokom kojeg su
razvijali i širili znanja na polju ovog dela fitopatologije. Uvođenjem fitobakteriologije u
nastavu na Poljoprivrednim fakultetima u Novom Sadu i Beogradu, zahvaljujući entuzijazmu i
naučnoj dalekovidosti profesora Momčila Arsenijevića i Milana Panića, ova oblast istraživanja
dobila je na intenzitetu i značaju. Poslednjih decenija razvoj fitobakteriologije tekao je
dinamično i ubrzano, zahvaljujući sve bržem napretku nauke i tehnologije, razvoju sredstava
komunikacije, posvećenosti istraživača, naučnoj saradnji, studijskim boravcima u
međunarodnim obrazovnim i naučnim institucijama, novim metodama izučavanja i
olakšanom pristupu informacijama. U Srbiji, najviše pažnje je posvećeno proučavanju FPB, a
kasnije i fitoplazmi, poreklom iz ekonomski značajnih biljnih vrsta voćaka, vinove loze,
povrtarskih, ratarskih i ukrasnih biljaka. Zahvaljujući interesovanju i entuzijazmu nevelikog
broja istraživača, usvajane su najsavremenije metode istraživanja ovih patogena.
Fitobakteriološka istraživanja su među prvima uvela u primenu molekularne metode u
domaćoj fitopatologiji. Primena PCR metode omogućila je razvoj nove epohe istraživanja
prokariota u Srbiji i upotrebu tehnika kao što su sekvenciranje genoma, analiza specifičnih ili
konstitutivnih gena, kvantitativni ili Real-time PCR, a to su metode koje se danas rutinski
koriste u istrživanjima prokariota u Srbiji. U novijem periodu razvijena su istraživanja u
oblasti detekcije, identifikacije i karakterizacije fitoplazmi u Srbiji, prateći ili predvodeći
svetska saznanja u ovoj oblasti. Primena savremenih metoda istraživanja omogućila je
izučavanje genetske strukture populacija fitopatogenih prokariota, detekciju novih vrsta u
našoj zemlji, stvaranje markera za precizniju identifikaciju ili proširivanje znanja iz oblasti
epidemiologije fitopatogenih prokariota. Osim toga, stečena saznanja se koriste u razvoju
inovativnih mogućnosti zaštite od bakterioza biološkim i konvencionalnim metodama. Decenijama sticano i unapređivano znanje neprekidno se prenosi generacijama koje dolaze,
kroz učešće fitobakteriologa u nastavnom procesu i kroz mentorski rad u visokoškolskim i
naučnim ustanovama, stvarajući tako osnov za dalji razvoj i napredak fitobakteriologije u
Srbiji.
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19
T1  - Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji
ER  - 

@conference{
author = "Obradović, Aleksa and Ivanović, Milan and Gašić, Katarina and Grahovac, Mila and Duduk, Bojan and Milijašević-Marčić, Svetlana and Ignjatov, Maja and Šević, Milan",
year = "2021",
abstract = "Istraživanja akademika Živojina Tešića iz 1934. godine, a zatim i prof. Dragoljuba Šutića
u prvoj deceniji posle drugog svetskog rata, na Poljoprivredno-šumarskom, kasnije
Poljoprivrednom fakultetu u Zemunu, mogu se smatrati početkom proučavanja fitopatogenih
bakterija (FPB) u Srbiji. Od tada do danas istraživači su prošli mukotrpan put tokom kojeg su
razvijali i širili znanja na polju ovog dela fitopatologije. Uvođenjem fitobakteriologije u
nastavu na Poljoprivrednim fakultetima u Novom Sadu i Beogradu, zahvaljujući entuzijazmu i
naučnoj dalekovidosti profesora Momčila Arsenijevića i Milana Panića, ova oblast istraživanja
dobila je na intenzitetu i značaju. Poslednjih decenija razvoj fitobakteriologije tekao je
dinamično i ubrzano, zahvaljujući sve bržem napretku nauke i tehnologije, razvoju sredstava
komunikacije, posvećenosti istraživača, naučnoj saradnji, studijskim boravcima u
međunarodnim obrazovnim i naučnim institucijama, novim metodama izučavanja i
olakšanom pristupu informacijama. U Srbiji, najviše pažnje je posvećeno proučavanju FPB, a
kasnije i fitoplazmi, poreklom iz ekonomski značajnih biljnih vrsta voćaka, vinove loze,
povrtarskih, ratarskih i ukrasnih biljaka. Zahvaljujući interesovanju i entuzijazmu nevelikog
broja istraživača, usvajane su najsavremenije metode istraživanja ovih patogena.
Fitobakteriološka istraživanja su među prvima uvela u primenu molekularne metode u
domaćoj fitopatologiji. Primena PCR metode omogućila je razvoj nove epohe istraživanja
prokariota u Srbiji i upotrebu tehnika kao što su sekvenciranje genoma, analiza specifičnih ili
konstitutivnih gena, kvantitativni ili Real-time PCR, a to su metode koje se danas rutinski
koriste u istrživanjima prokariota u Srbiji. U novijem periodu razvijena su istraživanja u
oblasti detekcije, identifikacije i karakterizacije fitoplazmi u Srbiji, prateći ili predvodeći
svetska saznanja u ovoj oblasti. Primena savremenih metoda istraživanja omogućila je
izučavanje genetske strukture populacija fitopatogenih prokariota, detekciju novih vrsta u
našoj zemlji, stvaranje markera za precizniju identifikaciju ili proširivanje znanja iz oblasti
epidemiologije fitopatogenih prokariota. Osim toga, stečena saznanja se koriste u razvoju
inovativnih mogućnosti zaštite od bakterioza biološkim i konvencionalnim metodama. Decenijama sticano i unapređivano znanje neprekidno se prenosi generacijama koje dolaze,
kroz učešće fitobakteriologa u nastavnom procesu i kroz mentorski rad u visokoškolskim i
naučnim ustanovama, stvarajući tako osnov za dalji razvoj i napredak fitobakteriologije u
Srbiji.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19",
title = "Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji"
}

Obradović, A., Ivanović, M., Gašić, K., Grahovac, M., Duduk, B., Milijašević-Marčić, S., Ignjatov, M.,& Šević, M.. (2021). Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19
Društvo za zaštitu bilja Srbije..

Obradović A, Ivanović M, Gašić K, Grahovac M, Duduk B, Milijašević-Marčić S, Ignjatov M, Šević M. Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19. 2021;..

Obradović, Aleksa, Ivanović, Milan, Gašić, Katarina, Grahovac, Mila, Duduk, Bojan, Milijašević-Marčić, Svetlana, Ignjatov, Maja, Šević, Milan, "Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19 (2021).

TY  - JOUR
AU  - Stefani, Emilio
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Altin, Irem
AU  - Nagy, Ildikó K.
AU  - Kovács, Tamás
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5851
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/610
AB  - Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.
PB  - MDPI
T2  - Microorganisms
T1  - Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future
IS  - 5
SP  - 1056
VL  - 9
DO  - 10.3390/microorganisms9051056
ER  - 

@article{
author = "Stefani, Emilio and Obradović, Aleksa and Gašić, Katarina and Altin, Irem and Nagy, Ildikó K. and Kovács, Tamás",
year = "2021",
abstract = "Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future",
number = "5",
pages = "1056",
volume = "9",
doi = "10.3390/microorganisms9051056"
}

Stefani, E., Obradović, A., Gašić, K., Altin, I., Nagy, I. K.,& Kovács, T.. (2021). Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms
MDPI., 9(5), 1056.
https://doi.org/10.3390/microorganisms9051056

Stefani E, Obradović A, Gašić K, Altin I, Nagy IK, Kovács T. Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms. 2021;9(5):1056.
doi:10.3390/microorganisms9051056 .

Stefani, Emilio, Obradović, Aleksa, Gašić, Katarina, Altin, Irem, Nagy, Ildikó K., Kovács, Tamás, "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future" in Microorganisms, 9, no. 5 (2021):1056,
https://doi.org/10.3390/microorganisms9051056 . .

TY  - CHAP
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B
AU  - Botond, Balogh
AU  - Gašić, Katarina
PY  - 2020-06
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/764
AB  - Lytic bacteriophages (phages) have the potential for controlling susceptible bacteria in the rhizosphere or phyllosphere. The success of phage application in plant disease control requires that high populations of both phage and bacterium exist in order to initiate a chain reaction of bacterial lysis. Physical factors in natural environments such as the presence of biofilms that trap phages, low soil pH which inactivates phages, low rates of diffusion of phages in soil that prevent contact with target bacteria, and inactivation of phages upon exposure to ultraviolet (UV) light and desiccation, all impact successful use of phages in control of plant pathogenic bacteria. Other considerations relate to the bacterial strains which exist in nature. The bacterial species may have a low or high degree of variation in sensitivity to phages. Therefore, phage selection for field use requires careful monitoring of the targeted bacterial strains in the field due to the potential for strain variation and the likelihood for development of resistance to the deployed phages. Application timing has also been shown to be an important factor in improving the efficacy of phages. For instance, UV light is deleterious to phages and upon exposure phage populations plummet; therefore, evening applications of phages result in persistence on leaf surfaces for longer periods of time and may result in improved disease control. Extending the period of time that phages persist in the phyllosphere has been a major hurdle. Formulations have been identified which improve the persistence of phages on leaf surfaces; however, there is a need to identify superior formulations that extend the life on leaf surfaces from hours to days. Another strategy for maintaining high populations of phages has been to use non-pathogenic bacterial strains that are sensitive to the phage(s) or a closely related organism that does not cause disease on the plant host. Finally, phages may have value as part of an integrated management strategy.
PB  - Caister Academic Press
T2  - In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.
T1  - Considerations for Using Bacteriophages in Plant Pathosystems
EP  - 282
SP  - 257
DO  - 10.21775/9781913652517.07
ER  - 

@inbook{
author = "Obradović, Aleksa and Jones, Jeffrey B and Botond, Balogh and Gašić, Katarina",
year = "2020-06",
abstract = "Lytic bacteriophages (phages) have the potential for controlling susceptible bacteria in the rhizosphere or phyllosphere. The success of phage application in plant disease control requires that high populations of both phage and bacterium exist in order to initiate a chain reaction of bacterial lysis. Physical factors in natural environments such as the presence of biofilms that trap phages, low soil pH which inactivates phages, low rates of diffusion of phages in soil that prevent contact with target bacteria, and inactivation of phages upon exposure to ultraviolet (UV) light and desiccation, all impact successful use of phages in control of plant pathogenic bacteria. Other considerations relate to the bacterial strains which exist in nature. The bacterial species may have a low or high degree of variation in sensitivity to phages. Therefore, phage selection for field use requires careful monitoring of the targeted bacterial strains in the field due to the potential for strain variation and the likelihood for development of resistance to the deployed phages. Application timing has also been shown to be an important factor in improving the efficacy of phages. For instance, UV light is deleterious to phages and upon exposure phage populations plummet; therefore, evening applications of phages result in persistence on leaf surfaces for longer periods of time and may result in improved disease control. Extending the period of time that phages persist in the phyllosphere has been a major hurdle. Formulations have been identified which improve the persistence of phages on leaf surfaces; however, there is a need to identify superior formulations that extend the life on leaf surfaces from hours to days. Another strategy for maintaining high populations of phages has been to use non-pathogenic bacterial strains that are sensitive to the phage(s) or a closely related organism that does not cause disease on the plant host. Finally, phages may have value as part of an integrated management strategy.",
publisher = "Caister Academic Press",
journal = "In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.",
booktitle = "Considerations for Using Bacteriophages in Plant Pathosystems",
pages = "282-257",
doi = "10.21775/9781913652517.07"
}

Obradović, A., Jones, J. B., Botond, B.,& Gašić, K.. (2020-06). Considerations for Using Bacteriophages in Plant Pathosystems. in In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.
Caister Academic Press., 257-282.
https://doi.org/10.21775/9781913652517.07

Obradović A, Jones JB, Botond B, Gašić K. Considerations for Using Bacteriophages in Plant Pathosystems. in In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.. 2020;:257-282.
doi:10.21775/9781913652517.07 .

Obradović, Aleksa, Jones, Jeffrey B, Botond, Balogh, Gašić, Katarina, "Considerations for Using Bacteriophages in Plant Pathosystems" in In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282. (2020-06):257-282,
https://doi.org/10.21775/9781913652517.07 . .

Popović Milovanović, T., Menković, J., Prokić, A.,& Obradović, A.. (2020). First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease
American Phytopathological Society., 104, 560-561.
https://doi.org/10.1094/PDIS-07-19-1422-PDN

Popović Milovanović T, Menković J, Prokić A, Obradović A. First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease. 2020;104:560-561.
doi:10.1094/PDIS-07-19-1422-PDN .

Popović Milovanović, Tatjana, Menković, J., Prokić, Anđelka, Obradović, Aleksa, "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro" in Plant Disease, 104 (2020):560-561,
https://doi.org/10.1094/PDIS-07-19-1422-PDN . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Pavlović, Z.
AU  - Obradović, Aleksa
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/592
AB  - Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia
EP  - 691
IS  - 3
SP  - 685
VL  - 157
DO  - 10.1007/s10658-020-02019-4
ER  - 

@article{
author = "Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Ivanović, Milan and Gašić, K. and Pavlović, Z. and Obradović, Aleksa",
year = "2020",
abstract = "Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia",
pages = "691-685",
number = "3",
volume = "157",
doi = "10.1007/s10658-020-02019-4"
}

Prokić, A., Zlatković, N., Kuzmanović, N., Ivanović, M., Gašić, K., Pavlović, Z.,& Obradović, A.. (2020). Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 157(3), 685-691.
https://doi.org/10.1007/s10658-020-02019-4

Prokić A, Zlatković N, Kuzmanović N, Ivanović M, Gašić K, Pavlović Z, Obradović A. Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology. 2020;157(3):685-691.
doi:10.1007/s10658-020-02019-4 .

Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Ivanović, Milan, Gašić, K., Pavlović, Z., Obradović, Aleksa, "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia" in European Journal of Plant Pathology, 157, no. 3 (2020):685-691,
https://doi.org/10.1007/s10658-020-02019-4 . .

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
American Phytopathological Society., 103(10), 2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Anđelka, Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

Gašić, K., Šević, M.,& Obradović, A.. (2019). Bakteriofag KF1 - novi biološki agens za zaštitu paprike od bakteriozne pegavosti. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Gašić K, Šević M, Obradović A. Bakteriofag KF1 - novi biološki agens za zaštitu paprike od bakteriozne pegavosti. in Institut za zaštitu bilja i životnu sredinu. 2019;..

Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Bakteriofag KF1 - novi biološki agens za zaštitu paprike od bakteriozne pegavosti" in Institut za zaštitu bilja i životnu sredinu (2019).

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1143
AB  - Watermelon (Citrullus lanatus) and squash (Cucurbita pepo) are traditionally grown in northern Serbia, in the province of Vojvodina. Most of the cucurbit cultivars grown in Serbia originate from imported seed. In July 2013, round, water-soaked lesions were observed on the fruits on sporadic squash plants grown in two fields of the breeder variety trials in Bački Petrovac. Affected tissue was light brown, slightly sunken, soft, and macerated. From symptomatic plants (cultivar GL Maxima), showing 30% symptom intensity, a uniform population of bacterial colonies was isolated, and one strain was chosen for the collection. In August of the following season, infection of the vine was observed on watermelon plants (cultivar unknown) grown in a commercial field in Čelarevo. Soft rot brownish lesions developed on infected stems, resulting in collapse and wilting of entire vines. The number of plants affected with symptoms was less than 10%. From the colonies isolated from the diseased tissue, two bacterial strains were purified and stored in the collection. Being pectolytic and isolated from cucurbits, the squash strain and two watermelon strains were subjected to further characterization. The strains developed white-gray, nonmucoid, round colonies with irregular margins on nutrient agar medium. They were gram and oxidase negative, tobacco HR and catalase positive, facultative anaerobes, nonfluorescent, caused soft rot on potato tuber slices, grew at 37°C, and reduced nitrate. They neither utilized arginine nor produced acid from sorbitol and inositol, showing characteristics of Pectobacterium species (De Boer and Kelman 2001). The PCR assay was carried out using primers BR1f/L1r (Duarte et al. 2004), specific for Pectobacterium carotovorum subsp. brasiliense (Pcb). A specific fragment of 322 bp was amplified for all three strains tested. The 16S rDNA sequence analysis (GenBank nos. MH031784, MH031785, and MH031786) showed 99% identity to the sequences of Pcb isolated from cucumber (CP020350), pepper (KX377597), and cabbage (KY021040) in China and potato in Japan (LC146476). The recA (MK911735, MK911736, and MK911737) and gapA (MK911738, MK911739, and MK911740) sequence analysis of three strains showed the highest nucleotide identity (100 to 98.35% and 99.42 to 98.08%, respectively) with Pcb previously deposited in NCBI GenBank database. Pathogenicity of the strains was tested on 2-week-old watermelon (cv. Rosa) and melon (cv. Sezam) seedlings, grown in a commercial potting mix in a greenhouse, as well as on mature zucchini fruits. Three seedlings per strain were inoculated by spraying with bacterial suspension (approx. 1 × 108 CFU/ml) using a handheld sprayer. Inoculated plants were incubated under plastic bags for 24 h and afterward maintained in a greenhouse at 28 ± 2°C. Two days after inoculation, water-soaked lesions developed on cotyledons of the watermelon and melon plants. No symptoms were observed on control plants sprayed with water. Three zucchini fruits were inoculated by pricking with a syringe and hypodermic needle and leaving a droplet of bacterial suspension (approx. 1 × 107 CFU/ml) at the point of inoculation. Inoculated fruits were placed on wet filter paper in a sealed plastic container at 28 ± 2°C. Treatment with sterile distilled water was used as a negative control. Discoloration of the tissue and soft rot spreading from the inoculation point developed within 48 h from inoculation. No symptoms developed on the control fruits. To complete Koch’s postulates, bacteria were reisolated from the inoculated plant and fruit tissue and were identified as Pcb by PCR assay (Duarte et al. 2004). This is the first report of Pcb causing soft rot of squash and watermelon in Serbia. The origin of the pathogen remained unknown. It might have been present in Serbia for some time but misidentified in previous studies that could not discriminate this subspecies or recently introduced by infected seed.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia
IS  - 10
SP  - 2667
VL  - 103
DO  - 10.1094/PDIS-12-18-2213-PDN
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Anđelka and Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Obradović, Aleksa",
year = "2019",
abstract = "Watermelon (Citrullus lanatus) and squash (Cucurbita pepo) are traditionally grown in northern Serbia, in the province of Vojvodina. Most of the cucurbit cultivars grown in Serbia originate from imported seed. In July 2013, round, water-soaked lesions were observed on the fruits on sporadic squash plants grown in two fields of the breeder variety trials in Bački Petrovac. Affected tissue was light brown, slightly sunken, soft, and macerated. From symptomatic plants (cultivar GL Maxima), showing 30% symptom intensity, a uniform population of bacterial colonies was isolated, and one strain was chosen for the collection. In August of the following season, infection of the vine was observed on watermelon plants (cultivar unknown) grown in a commercial field in Čelarevo. Soft rot brownish lesions developed on infected stems, resulting in collapse and wilting of entire vines. The number of plants affected with symptoms was less than 10%. From the colonies isolated from the diseased tissue, two bacterial strains were purified and stored in the collection. Being pectolytic and isolated from cucurbits, the squash strain and two watermelon strains were subjected to further characterization. The strains developed white-gray, nonmucoid, round colonies with irregular margins on nutrient agar medium. They were gram and oxidase negative, tobacco HR and catalase positive, facultative anaerobes, nonfluorescent, caused soft rot on potato tuber slices, grew at 37°C, and reduced nitrate. They neither utilized arginine nor produced acid from sorbitol and inositol, showing characteristics of Pectobacterium species (De Boer and Kelman 2001). The PCR assay was carried out using primers BR1f/L1r (Duarte et al. 2004), specific for Pectobacterium carotovorum subsp. brasiliense (Pcb). A specific fragment of 322 bp was amplified for all three strains tested. The 16S rDNA sequence analysis (GenBank nos. MH031784, MH031785, and MH031786) showed 99% identity to the sequences of Pcb isolated from cucumber (CP020350), pepper (KX377597), and cabbage (KY021040) in China and potato in Japan (LC146476). The recA (MK911735, MK911736, and MK911737) and gapA (MK911738, MK911739, and MK911740) sequence analysis of three strains showed the highest nucleotide identity (100 to 98.35% and 99.42 to 98.08%, respectively) with Pcb previously deposited in NCBI GenBank database. Pathogenicity of the strains was tested on 2-week-old watermelon (cv. Rosa) and melon (cv. Sezam) seedlings, grown in a commercial potting mix in a greenhouse, as well as on mature zucchini fruits. Three seedlings per strain were inoculated by spraying with bacterial suspension (approx. 1 × 108 CFU/ml) using a handheld sprayer. Inoculated plants were incubated under plastic bags for 24 h and afterward maintained in a greenhouse at 28 ± 2°C. Two days after inoculation, water-soaked lesions developed on cotyledons of the watermelon and melon plants. No symptoms were observed on control plants sprayed with water. Three zucchini fruits were inoculated by pricking with a syringe and hypodermic needle and leaving a droplet of bacterial suspension (approx. 1 × 107 CFU/ml) at the point of inoculation. Inoculated fruits were placed on wet filter paper in a sealed plastic container at 28 ± 2°C. Treatment with sterile distilled water was used as a negative control. Discoloration of the tissue and soft rot spreading from the inoculation point developed within 48 h from inoculation. No symptoms developed on the control fruits. To complete Koch’s postulates, bacteria were reisolated from the inoculated plant and fruit tissue and were identified as Pcb by PCR assay (Duarte et al. 2004). This is the first report of Pcb causing soft rot of squash and watermelon in Serbia. The origin of the pathogen remained unknown. It might have been present in Serbia for some time but misidentified in previous studies that could not discriminate this subspecies or recently introduced by infected seed.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia",
number = "10",
pages = "2667",
volume = "103",
doi = "10.1094/PDIS-12-18-2213-PDN"
}

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
American Phytopathological Society., 103(10), 2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Anđelka, Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Newberry, Eric
AU  - Ebrahim, Mohamed
AU  - Timilsina, Sujan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
AU  - Bull, Carolee
AU  - Goss, Erica
AU  - Huguet Tapia, Jose
AU  - Paret, Mathews
AU  - Jones, Jeffrey
AU  - Potnis, Neha
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/960
AB  - Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash
EP  - 18
SP  - 1
VL  - 10
DO  - 10.3389/fmicb.2019.00270
ER  - 

@article{
author = "Newberry, Eric and Ebrahim, Mohamed and Timilsina, Sujan and Zlatković, Nevena and Obradović, Aleksa and Bull, Carolee and Goss, Erica and Huguet Tapia, Jose and Paret, Mathews and Jones, Jeffrey and Potnis, Neha",
year = "2019",
abstract = "Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash",
pages = "18-1",
volume = "10",
doi = "10.3389/fmicb.2019.00270"
}

Newberry, E., Ebrahim, M., Timilsina, S., Zlatković, N., Obradović, A., Bull, C., Goss, E., Huguet Tapia, J., Paret, M., Jones, J.,& Potnis, N.. (2019). Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10, 1-18.
https://doi.org/10.3389/fmicb.2019.00270

Newberry E, Ebrahim M, Timilsina S, Zlatković N, Obradović A, Bull C, Goss E, Huguet Tapia J, Paret M, Jones J, Potnis N. Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology. 2019;10:1-18.
doi:10.3389/fmicb.2019.00270 .

Newberry, Eric, Ebrahim, Mohamed, Timilsina, Sujan, Zlatković, Nevena, Obradović, Aleksa, Bull, Carolee, Goss, Erica, Huguet Tapia, Jose, Paret, Mathews, Jones, Jeffrey, Potnis, Neha, "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash" in Frontiers in Microbiology, 10 (2019):1-18,
https://doi.org/10.3389/fmicb.2019.00270 . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/576
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .