TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Biondi, E.
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Zlatković, Nevena
AU  - Bertaccini, Assunta
AU  - Obradović, Aleksa
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/862
AB  - Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.
PB  - Springer
T2  - Journal of Plant Pathology
T1  - Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall
EP  - 319
IS  - 2
SP  - 311
VL  - 98
DO  - 10.4454/JPP.V98I2.028
ER  - 

@article{
author = "Kuzmanović, Nemanja and Biondi, E. and Ivanović, Milan and Prokić, Anđelka and Zlatković, Nevena and Bertaccini, Assunta and Obradović, Aleksa",
year = "2016",
abstract = "Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.",
publisher = "Springer",
journal = "Journal of Plant Pathology",
title = "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall",
pages = "319-311",
number = "2",
volume = "98",
doi = "10.4454/JPP.V98I2.028"
}

Kuzmanović, N., Biondi, E., Ivanović, M., Prokić, A., Zlatković, N., Bertaccini, A.,& Obradović, A.. (2016). Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology
Springer., 98(2), 311-319.
https://doi.org/10.4454/JPP.V98I2.028

Kuzmanović N, Biondi E, Ivanović M, Prokić A, Zlatković N, Bertaccini A, Obradović A. Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology. 2016;98(2):311-319.
doi:10.4454/JPP.V98I2.028 .

Kuzmanović, Nemanja, Biondi, E., Ivanović, Milan, Prokić, Anđelka, Zlatković, Nevena, Bertaccini, Assunta, Obradović, Aleksa, "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall" in Journal of Plant Pathology, 98, no. 2 (2016):311-319,
https://doi.org/10.4454/JPP.V98I2.028 . .