TY  - JOUR
AU  - Atanasova, Biljana
AU  - Jakovljević, Miljana
AU  - Spasov, Dušan
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/383
AB  - Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia
EP  - 770
IS  - 4
SP  - 759
VL  - 142
DO  - 10.1007/s10658-015-0649-0
ER  - 

@article{
author = "Atanasova, Biljana and Jakovljević, Miljana and Spasov, Dušan and Jović, Jelena and Mitrović, Milana and Toševski, Ivo and Cvrković, Tatjana",
year = "2015",
abstract = "Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia",
pages = "770-759",
number = "4",
volume = "142",
doi = "10.1007/s10658-015-0649-0"
}

Atanasova, B., Jakovljević, M., Spasov, D., Jović, J., Mitrović, M., Toševski, I.,& Cvrković, T.. (2015). The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology
Springer, Dordrecht., 142(4), 759-770.
https://doi.org/10.1007/s10658-015-0649-0

Atanasova B, Jakovljević M, Spasov D, Jović J, Mitrović M, Toševski I, Cvrković T. The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology. 2015;142(4):759-770.
doi:10.1007/s10658-015-0649-0 .

Atanasova, Biljana, Jakovljević, Miljana, Spasov, Dušan, Jović, Jelena, Mitrović, Milana, Toševski, Ivo, Cvrković, Tatjana, "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia" in European Journal of Plant Pathology, 142, no. 4 (2015):759-770,
https://doi.org/10.1007/s10658-015-0649-0 . .

TY  - JOUR
AU  - Atanasova, Biljana
AU  - Spasov, Dušan
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/338
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Alder Yellows Phytoplasma Associated with Common Alder (Alnus glutinosa) in the Republic of Macedonia.
EP  - 1268
IS  - 9
SP  - 1268
VL  - 98
DO  - 10.1094/PDIS-03-14-0315-PDN
ER  - 

@article{
author = "Atanasova, Biljana and Spasov, Dušan and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Alder Yellows Phytoplasma Associated with Common Alder (Alnus glutinosa) in the Republic of Macedonia.",
pages = "1268-1268",
number = "9",
volume = "98",
doi = "10.1094/PDIS-03-14-0315-PDN"
}

Atanasova, B., Spasov, D., Jakovljević, M., Jović, J., Krstić, O., Mitrović, M.,& Cvrković, T.. (2014). First Report of Alder Yellows Phytoplasma Associated with Common Alder (Alnus glutinosa) in the Republic of Macedonia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(9), 1268-1268.
https://doi.org/10.1094/PDIS-03-14-0315-PDN

Atanasova B, Spasov D, Jakovljević M, Jović J, Krstić O, Mitrović M, Cvrković T. First Report of Alder Yellows Phytoplasma Associated with Common Alder (Alnus glutinosa) in the Republic of Macedonia.. in Plant Disease. 2014;98(9):1268-1268.
doi:10.1094/PDIS-03-14-0315-PDN .

Atanasova, Biljana, Spasov, Dušan, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, "First Report of Alder Yellows Phytoplasma Associated with Common Alder (Alnus glutinosa) in the Republic of Macedonia." in Plant Disease, 98, no. 9 (2014):1268-1268,
https://doi.org/10.1094/PDIS-03-14-0315-PDN . .