TY  - JOUR
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/565
AB  - Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia
EP  - 195
IS  - 1
SP  - 183
VL  - 153
DO  - 10.1007/s10658-018-1553-1
ER  - 

@article{
author = "Kosovac, Andrea and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2019",
abstract = "Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia",
pages = "195-183",
number = "1",
volume = "153",
doi = "10.1007/s10658-018-1553-1"
}

Kosovac, A., Jakovljević, M., Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2019). Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 153(1), 183-195.
https://doi.org/10.1007/s10658-018-1553-1

Kosovac A, Jakovljević M, Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology. 2019;153(1):183-195.
doi:10.1007/s10658-018-1553-1 .

Kosovac, Andrea, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia" in European Journal of Plant Pathology, 153, no. 1 (2019):183-195,
https://doi.org/10.1007/s10658-018-1553-1 . .

TY  - THES
AU  - Kosovac, Andrea
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6105
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18540/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025193650
UR  - http://nardus.mpn.gov.rs/123456789/10053
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/572
AB  - Hyalesthes obsoletus, vektor biljnog patogena 'Candidatus (Ca.) Phytoplasmasolani', smatra se polifagnom štetočinom od ekonomskog značaja u centralnojEvropi i Mediteranu. Istraživanjima sprovedenim u jugoistočnoj Evropi, kojagravitira centru arela vrste i gde je zabeležen značajan broj 'Ca. P. solani'-uslovljenih biljnih bolesti, utvrđeno je postojanje četiri asocijacije vektora sabiljkama dualnim domaćinima: Convolvulus arvensis (Ca), Urtica dioica (Ud), Vitexagnus-castus (Vac) i Crepis foetida (Cf). Populaciono-genetičkim analizamamitohondrijskih i nuklearnih markera (mikrosatelita) utvrđena je kriptičnadiferencijacija simpatričkih (uključujući sintopske) i geografski udaljenih H.obsoletus populacija na tri filogenetičke linije uslovljene biljkom domaćinom: (1)Ca-Ud, (2) Vac i (3) Cf. Pored jasne diferencijacije na mitohondrijskim genima(1.1% - 1.5%) i mikrosatelita koji potvrđuju izdvajanje populacija u jedan od triklastera prema biljci domaćinu (pripadnost >90%), podršku za izdizanje genetičkedivergencije H. obsoletus sensu lato na nivo kriptične specijacije daje registrovanadistanca u odnosu na morfološki najsrodniju vrstu - Hyalesthes thracicus (2.1% -3.3%). Posledice specijalizacije i diferencijacije H. obsoletus sensu lato premabiljkama domaćinima na epidemiologiju 'Ca. P. solani' procenjene su multigenskomkarakterizacijom izolata fitoplazme detektovanih (1) in situ u prirodnimepidemiološkim sistemima mediteranskih vinograda i (2) eksperimentimaunakrsne inokulacije biljaka putem prirodno inficiranih populacija vektora.Potvrđena je osnovna epidemiološka divergencija 'Ca. P. solani' na tuf-a/-ab i tuf-bcikluse vezane za Ud i Ca uz ukrštanje sa Vac-uzrokovanom tuf-b epidemiologijom iutvrđena kompleksnost tuf-b epidemiologije u kojoj učestvuje i treća genetičkalinija vektora, H. obsoletus ex Cf, kao i sama biljka dualni domaćin - C. foetida.
AB  - The cixiid planthopper Hyalesthes obsoletus is generally considered as apolyphagous vector of 'Candidatus (Ca.) P. solani' associated with numerous wildand cultivated plants in central Europe and Mediterranean. Research conducted insoutheastern Europe, the distribution centre of H. obsoletus and the area of most'Ca. P. solani'-inflicted crop diseases, points toward specific dual host-plantassociations with: Convolvulus arvensis (Ca), Urtica dioica (Ud), Vitex agnus-castus(Vac) and Crepis foetida (Cf). Population-genetic analysis of mitochondrial andnuclear genetic markers (microsatellites) have revealed cryptic differentiationbetween sympatric (including syntopic) and geographically distant H. obsoletuspopulations into three phylogenetic lineages delineated by host-plant preference:1) Ca-Ud, 2) Vac i 3) Cf. Cryptic species diversification within H. obsoletus sensu latois supported by the genetic divergence of mitochondrial genes (1.1% - 1.5%),microsatellites that confirm population clustering into three genetic groupsassociated with specific host-plant (average assignment >90%) and geneticdistances among host-associated H. obsoletus metapopulations comparable to themost closely related, morphologically distinguishable species - Hyalesthes thracicus(2.1% - 3.3%). Consequences of the H. obsoletus sensu lato differentiation on theepidemiology of 'Ca. P. solani' are assessed by multilocus genotyping of thephytoplasma isolates detected (1) in situ in natural epidemiological systems of theMediterranean vineyards and (2) plant inoculation experiments using naturallyinfected vector populations. Basic 'Ca. P. solani' epidemiological divergence intotuf-a/-ab and tuf-b cycles, each associated with Ud and Ca, respectively, isconfirmed with evidences of intermixing Vac-induced tuf-b epidemiology, whilecomplexity of the tuf-b epidemiology is further evidenced through influence of thethird genetic lineage of the vector H. obsoletus ex C. foetida and its dual host-plant.
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10053
ER  - 

@phdthesis{
author = "Kosovac, Andrea",
year = "2018",
abstract = "Hyalesthes obsoletus, vektor biljnog patogena 'Candidatus (Ca.) Phytoplasmasolani', smatra se polifagnom štetočinom od ekonomskog značaja u centralnojEvropi i Mediteranu. Istraživanjima sprovedenim u jugoistočnoj Evropi, kojagravitira centru arela vrste i gde je zabeležen značajan broj 'Ca. P. solani'-uslovljenih biljnih bolesti, utvrđeno je postojanje četiri asocijacije vektora sabiljkama dualnim domaćinima: Convolvulus arvensis (Ca), Urtica dioica (Ud), Vitexagnus-castus (Vac) i Crepis foetida (Cf). Populaciono-genetičkim analizamamitohondrijskih i nuklearnih markera (mikrosatelita) utvrđena je kriptičnadiferencijacija simpatričkih (uključujući sintopske) i geografski udaljenih H.obsoletus populacija na tri filogenetičke linije uslovljene biljkom domaćinom: (1)Ca-Ud, (2) Vac i (3) Cf. Pored jasne diferencijacije na mitohondrijskim genima(1.1% - 1.5%) i mikrosatelita koji potvrđuju izdvajanje populacija u jedan od triklastera prema biljci domaćinu (pripadnost >90%), podršku za izdizanje genetičkedivergencije H. obsoletus sensu lato na nivo kriptične specijacije daje registrovanadistanca u odnosu na morfološki najsrodniju vrstu - Hyalesthes thracicus (2.1% -3.3%). Posledice specijalizacije i diferencijacije H. obsoletus sensu lato premabiljkama domaćinima na epidemiologiju 'Ca. P. solani' procenjene su multigenskomkarakterizacijom izolata fitoplazme detektovanih (1) in situ u prirodnimepidemiološkim sistemima mediteranskih vinograda i (2) eksperimentimaunakrsne inokulacije biljaka putem prirodno inficiranih populacija vektora.Potvrđena je osnovna epidemiološka divergencija 'Ca. P. solani' na tuf-a/-ab i tuf-bcikluse vezane za Ud i Ca uz ukrštanje sa Vac-uzrokovanom tuf-b epidemiologijom iutvrđena kompleksnost tuf-b epidemiologije u kojoj učestvuje i treća genetičkalinija vektora, H. obsoletus ex Cf, kao i sama biljka dualni domaćin - C. foetida., The cixiid planthopper Hyalesthes obsoletus is generally considered as apolyphagous vector of 'Candidatus (Ca.) P. solani' associated with numerous wildand cultivated plants in central Europe and Mediterranean. Research conducted insoutheastern Europe, the distribution centre of H. obsoletus and the area of most'Ca. P. solani'-inflicted crop diseases, points toward specific dual host-plantassociations with: Convolvulus arvensis (Ca), Urtica dioica (Ud), Vitex agnus-castus(Vac) and Crepis foetida (Cf). Population-genetic analysis of mitochondrial andnuclear genetic markers (microsatellites) have revealed cryptic differentiationbetween sympatric (including syntopic) and geographically distant H. obsoletuspopulations into three phylogenetic lineages delineated by host-plant preference:1) Ca-Ud, 2) Vac i 3) Cf. Cryptic species diversification within H. obsoletus sensu latois supported by the genetic divergence of mitochondrial genes (1.1% - 1.5%),microsatellites that confirm population clustering into three genetic groupsassociated with specific host-plant (average assignment >90%) and geneticdistances among host-associated H. obsoletus metapopulations comparable to themost closely related, morphologically distinguishable species - Hyalesthes thracicus(2.1% - 3.3%). Consequences of the H. obsoletus sensu lato differentiation on theepidemiology of 'Ca. P. solani' are assessed by multilocus genotyping of thephytoplasma isolates detected (1) in situ in natural epidemiological systems of theMediterranean vineyards and (2) plant inoculation experiments using naturallyinfected vector populations. Basic 'Ca. P. solani' epidemiological divergence intotuf-a/-ab and tuf-b cycles, each associated with Ud and Ca, respectively, isconfirmed with evidences of intermixing Vac-induced tuf-b epidemiology, whilecomplexity of the tuf-b epidemiology is further evidenced through influence of thethird genetic lineage of the vector H. obsoletus ex C. foetida and its dual host-plant.",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10053"
}

Kosovac, A.. (2018). Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_10053

Kosovac A. Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10053 .

Kosovac, Andrea, "Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10053 .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/547
AB  - The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).
PB  - Public Library of Science (PLoS)
T2  - PlOS One
T1  - Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation
IS  - 5
VL  - 13
DO  - 10.1371/journal.pone.0196969
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).",
publisher = "Public Library of Science (PLoS)",
journal = "PlOS One",
title = "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation",
number = "5",
volume = "13",
doi = "10.1371/journal.pone.0196969"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2018). Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One
Public Library of Science (PLoS)., 13(5).
https://doi.org/10.1371/journal.pone.0196969

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One. 2018;13(5).
doi:10.1371/journal.pone.0196969 .

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation" in PlOS One, 13, no. 5 (2018),
https://doi.org/10.1371/journal.pone.0196969 . .

TY  - JOUR
AU  - Stepanović, Svetlana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/445
AB  - Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.
PB  - Wiley, Hoboken
T2  - Insect Science
T1  - Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification
EP  - 648
IS  - 4
SP  - 638
VL  - 23
DO  - 10.1111/1744-7917.12212
ER  - 

@article{
author = "Stepanović, Svetlana and Kosovac, Andrea and Krstić, Oliver and Jović, Jelena and Toševski, Ivo",
year = "2016",
abstract = "Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.",
publisher = "Wiley, Hoboken",
journal = "Insect Science",
title = "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification",
pages = "648-638",
number = "4",
volume = "23",
doi = "10.1111/1744-7917.12212"
}

Stepanović, S., Kosovac, A., Krstić, O., Jović, J.,& Toševski, I.. (2016). Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science
Wiley, Hoboken., 23(4), 638-648.
https://doi.org/10.1111/1744-7917.12212

Stepanović S, Kosovac A, Krstić O, Jović J, Toševski I. Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science. 2016;23(4):638-648.
doi:10.1111/1744-7917.12212 .

Stepanović, Svetlana, Kosovac, Andrea, Krstić, Oliver, Jović, Jelena, Toševski, Ivo, "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification" in Insect Science, 23, no. 4 (2016):638-648,
https://doi.org/10.1111/1744-7917.12212 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/428
AB  - A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors
EP  - 630
IS  - 3
SP  - 619
VL  - 144
DO  - 10.1007/s10658-015-0800-y
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Jermini, Mauro and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
abstract = "A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors",
pages = "630-619",
number = "3",
volume = "144",
doi = "10.1007/s10658-015-0800-y"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Jermini, M., Toševski, I.,& Cvrković, T.. (2016). 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology
Springer, Dordrecht., 144(3), 619-630.
https://doi.org/10.1007/s10658-015-0800-y

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Jermini M, Toševski I, Cvrković T. 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology. 2016;144(3):619-630.
doi:10.1007/s10658-015-0800-y .

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Jermini, Mauro, Toševski, Ivo, Cvrković, Tatjana, "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors" in European Journal of Plant Pathology, 144, no. 3 (2016):619-630,
https://doi.org/10.1007/s10658-015-0800-y . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/439
AB  - Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)
EP  - 298
IS  - 2
SP  - 285
VL  - 65
DO  - 10.1111/ppa.12409
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)",
pages = "298-285",
number = "2",
volume = "65",
doi = "10.1111/ppa.12409"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology
Wiley, Hoboken., 65(2), 285-298.
https://doi.org/10.1111/ppa.12409

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology. 2016;65(2):285-298.
doi:10.1111/ppa.12409 .

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)" in Plant Pathology, 65, no. 2 (2016):285-298,
https://doi.org/10.1111/ppa.12409 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/430
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards
EP  - 3
IS  - 1
SP  - 1
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards",
pages = "3-1",
number = "1",
volume = "66"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 1-3.

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards. in Mitteilungen Klosterneuburg. 2016;66(1):1-3..

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):1-3.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/431
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data
EP  - 11
IS  - 1
SP  - 9
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Krstić, Oliver and Jakovljević, Miljana and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data",
pages = "11-9",
number = "1",
volume = "66"
}

Kosovac, A., Krstić, O., Jakovljević, M., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 9-11.

Kosovac A, Krstić O, Jakovljević M, Cvrković T, Mitrović M, Toševski I, Jović J. Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg. 2016;66(1):9-11..

Kosovac, Andrea, Krstić, Oliver, Jakovljević, Miljana, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):9-11.

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/432
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia
EP  - 18
IS  - 1
SP  - 17
VL  - 66
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia",
pages = "18-17",
number = "1",
volume = "66"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Toševski, I.,& Cvrković, T.. (2016). Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 17-18.

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Toševski I, Cvrković T. Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg. 2016;66(1):17-18..

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Toševski, Ivo, Cvrković, Tatjana, "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):17-18.

TY  - JOUR
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Mitrović, Milana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/433
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland
EP  - 23
IS  - 1
SP  - 22
VL  - 66
ER  - 

@article{
author = "Trivellone, Valeria and Jermini, Mauro and Jović, Jelena and Cvrković, Tatjana and Jakovljević, Miljana and Kosovac, Andrea and Krstić, Oliver and Toševski, Ivo and Mitrović, Milana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland",
pages = "23-22",
number = "1",
volume = "66"
}

Trivellone, V., Jermini, M., Jović, J., Cvrković, T., Jakovljević, M., Kosovac, A., Krstić, O., Toševski, I.,& Mitrović, M.. (2016). Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 22-23.

Trivellone V, Jermini M, Jović J, Cvrković T, Jakovljević M, Kosovac A, Krstić O, Toševski I, Mitrović M. Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg. 2016;66(1):22-23..

Trivellone, Valeria, Jermini, Mauro, Jović, Jelena, Cvrković, Tatjana, Jakovljević, Miljana, Kosovac, Andrea, Krstić, Oliver, Toševski, Ivo, Mitrović, Milana, "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):22-23.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/434
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe
EP  - 25
IS  - 1
SP  - 24
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe",
pages = "25-24",
number = "1",
volume = "66"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2016). Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 24-25.

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg. 2016;66(1):24-25..

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):24-25.

TY  - JOUR
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/444
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro
EP  - 1775
IS  - 8
SP  - 1775
VL  - 100
DO  - 10.1094/PDIS-02-16-0180-PDN
ER  - 

@article{
author = "Radonjić, Sanja and Hrnčić, Snježana and Kosovac, Andrea and Krstić, Oliver and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro",
pages = "1775-1775",
number = "8",
volume = "100",
doi = "10.1094/PDIS-02-16-0180-PDN"
}

Radonjić, S., Hrnčić, S., Kosovac, A., Krstić, O., Mitrović, M., Jović, J.,& Toševski, I.. (2016). First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(8), 1775-1775.
https://doi.org/10.1094/PDIS-02-16-0180-PDN

Radonjić S, Hrnčić S, Kosovac A, Krstić O, Mitrović M, Jović J, Toševski I. First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro. in Plant Disease. 2016;100(8):1775-1775.
doi:10.1094/PDIS-02-16-0180-PDN .

Radonjić, Sanja, Hrnčić, Snježana, Kosovac, Andrea, Krstić, Oliver, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of 'Candidatus Phytoplasma solani' Associated With Potato Stolbur Disease in Montenegro" in Plant Disease, 100, no. 8 (2016):1775-1775,
https://doi.org/10.1094/PDIS-02-16-0180-PDN . .

TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/380
AB  - We investigated multiple inflorescence disease of Cirsium arvense (CMI) and its association with phytoplasmas of the 16SrIII-B subgroup, potential natural vector(s) and reservoir plant(s). From five locations in northern Serbia, 27 plants of C. arvense, 1 C. vulgare and 3 Carduus acanthoides with symptoms of multiple inflorescences (MIs) were collected and tested for 16SrIII group phytoplasmas. All symptomatic plants were found to be infected. Tentative reservoir plants and insect vectors were collected at a Dobanovci site where the continuous presence of CMI disease was recorded. Among the 19 most abundant plant species submitted to phytoplasma testing, all symptomless, the presence of the 16SrIII group was detected only in two legumes: Lathyrus tuberosus (2/5) and L. aphaca (1/5). Among 19 insect species from six families of Auchenorrhyncha, the deltocephalid leafhopper Euscelis incisus was the only insect carrying a 16SrIII phytoplasma (10% of analysed individuals). Transmission trials were performed with naturally infected E. incisus adults of the summer generation and with a laboratory population reared on red clover. After an acquisition period of 48 h on C. arvense symptomatic for MIs and a latent period of 28 days, 83% of the E. incisus adults (300/360) were infected with CMI phytoplasma. In two transmission tests, the leafhoppers successfully transmitted the phytoplasma to exposed plants (C. arvense and periwinkle), proving its role as a natural vector. Test plants of C. arvense infected with the 16SrIII-B phytoplasma expressed typical symptoms similar to those observed in the field, such as MIs or the absence of flowering, shortened internodes and plant desiccation. Typical symptoms in infected periwinkles were virescence and phyllody. The molecular characterisation of the CMI phytoplasma isolates from diseased and asymptomatic field-collected plants, vectors, and test plants was performed by sequence analyses of the 16S rRNA, rpl22-rps3 and rpl15-secY genes. Phylogenetic analyses of other members of the 16SrIII group of phytoplasmas indicated closest relatedness with clover yellow edge phytoplasma (CYE) of the 16SrIII-B subgroup.
PB  - Wiley-Blackwell, Hoboken
T2  - Annals of Applied Biology
T1  - Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense
EP  - 419
IS  - 3
SP  - 406
VL  - 167
DO  - 10.1111/aab.12236
ER  - 

@article{
author = "Jakovljević, Miljana and Jović, Jelena and Mitrović, Milana and Krstić, Oliver and Kosovac, Andrea and Toševski, Ivo and Cvrković, Tatjana",
year = "2015",
abstract = "We investigated multiple inflorescence disease of Cirsium arvense (CMI) and its association with phytoplasmas of the 16SrIII-B subgroup, potential natural vector(s) and reservoir plant(s). From five locations in northern Serbia, 27 plants of C. arvense, 1 C. vulgare and 3 Carduus acanthoides with symptoms of multiple inflorescences (MIs) were collected and tested for 16SrIII group phytoplasmas. All symptomatic plants were found to be infected. Tentative reservoir plants and insect vectors were collected at a Dobanovci site where the continuous presence of CMI disease was recorded. Among the 19 most abundant plant species submitted to phytoplasma testing, all symptomless, the presence of the 16SrIII group was detected only in two legumes: Lathyrus tuberosus (2/5) and L. aphaca (1/5). Among 19 insect species from six families of Auchenorrhyncha, the deltocephalid leafhopper Euscelis incisus was the only insect carrying a 16SrIII phytoplasma (10% of analysed individuals). Transmission trials were performed with naturally infected E. incisus adults of the summer generation and with a laboratory population reared on red clover. After an acquisition period of 48 h on C. arvense symptomatic for MIs and a latent period of 28 days, 83% of the E. incisus adults (300/360) were infected with CMI phytoplasma. In two transmission tests, the leafhoppers successfully transmitted the phytoplasma to exposed plants (C. arvense and periwinkle), proving its role as a natural vector. Test plants of C. arvense infected with the 16SrIII-B phytoplasma expressed typical symptoms similar to those observed in the field, such as MIs or the absence of flowering, shortened internodes and plant desiccation. Typical symptoms in infected periwinkles were virescence and phyllody. The molecular characterisation of the CMI phytoplasma isolates from diseased and asymptomatic field-collected plants, vectors, and test plants was performed by sequence analyses of the 16S rRNA, rpl22-rps3 and rpl15-secY genes. Phylogenetic analyses of other members of the 16SrIII group of phytoplasmas indicated closest relatedness with clover yellow edge phytoplasma (CYE) of the 16SrIII-B subgroup.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Annals of Applied Biology",
title = "Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense",
pages = "419-406",
number = "3",
volume = "167",
doi = "10.1111/aab.12236"
}

Jakovljević, M., Jović, J., Mitrović, M., Krstić, O., Kosovac, A., Toševski, I.,& Cvrković, T.. (2015). Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense. in Annals of Applied Biology
Wiley-Blackwell, Hoboken., 167(3), 406-419.
https://doi.org/10.1111/aab.12236

Jakovljević M, Jović J, Mitrović M, Krstić O, Kosovac A, Toševski I, Cvrković T. Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense. in Annals of Applied Biology. 2015;167(3):406-419.
doi:10.1111/aab.12236 .

Jakovljević, Miljana, Jović, Jelena, Mitrović, Milana, Krstić, Oliver, Kosovac, Andrea, Toševski, Ivo, Cvrković, Tatjana, "Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16SrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense" in Annals of Applied Biology, 167, no. 3 (2015):406-419,
https://doi.org/10.1111/aab.12236 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/400
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of 'Candidatus Phytoplasma solani' Infecting Garden Bean Phaseolus vulgaris in Serbia
EP  - 551
IS  - 4
SP  - 551
VL  - 99
DO  - 10.1094/PDIS-10-14-1052-PDN
ER  - 

@article{
author = "Mitrović, Milana and Cvrković, Tatjana and Jović, Jelena and Krstić, Oliver and Jakovljević, Miljana and Kosovac, Andrea and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of 'Candidatus Phytoplasma solani' Infecting Garden Bean Phaseolus vulgaris in Serbia",
pages = "551-551",
number = "4",
volume = "99",
doi = "10.1094/PDIS-10-14-1052-PDN"
}

Mitrović, M., Cvrković, T., Jović, J., Krstić, O., Jakovljević, M., Kosovac, A.,& Toševski, I.. (2015). First Report of 'Candidatus Phytoplasma solani' Infecting Garden Bean Phaseolus vulgaris in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(4), 551-551.
https://doi.org/10.1094/PDIS-10-14-1052-PDN

Mitrović M, Cvrković T, Jović J, Krstić O, Jakovljević M, Kosovac A, Toševski I. First Report of 'Candidatus Phytoplasma solani' Infecting Garden Bean Phaseolus vulgaris in Serbia. in Plant Disease. 2015;99(4):551-551.
doi:10.1094/PDIS-10-14-1052-PDN .

Mitrović, Milana, Cvrković, Tatjana, Jović, Jelena, Krstić, Oliver, Jakovljević, Miljana, Kosovac, Andrea, Toševski, Ivo, "First Report of 'Candidatus Phytoplasma solani' Infecting Garden Bean Phaseolus vulgaris in Serbia" in Plant Disease, 99, no. 4 (2015):551-551,
https://doi.org/10.1094/PDIS-10-14-1052-PDN . .

TY  - JOUR
AU  - Toševski, Ivo
AU  - Milenković, Slobodan
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Jović, Jelena
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/303
AB  - During October and November 2014, a survey was conducted in order to establish the presence of the invasive pest Drosophila suzukii (Matsumura, 1931) on the territory of Serbia. Survey revealed the presence of this fly in four districts (Rasinski, Mačvanski, Raški and Pčinjski), in sampled raspberry and blackberry ripe fruits, as well as in common fig and grape. Collected fruits were used for the rearing of Drosophilid larvae to adults, which were subsequently subjected to morphological and molecular characterization. The presence of D. suzukii was confirmed in all surveyed districts, as well as in Zemun (City of Belgrade). This is the first report of the highly invasive fruit pest D. suzukii on the territory of Serbia.
AB  - Tokom oktobra i novembra 2014. godine, sprovedena su istraživanja u cilju utvrđivanja prisustva i rasprostranjenja invazivne štetočine Drosophila suzukii (Matsumura, 1931) na teritoriji Srbije. Prisustvo ove mušice je utvrđeno u okviru četiri okruga (Rasinski, Mačvanski, Raški, Pčinjski), u uzorkovanim plodovima maline, kupine, smokve i grožđa. Iz plodova su odgajene Drozofilidne larve do adulta koji su zatim analizirani morfološki i molekularno. Prisustvo D. suzukii je utvrđeno na svim lokalitetima u okviru četiri okruga, kao i na teritoriji grada Beograda (Zemun). Ovo je prvi nalaz invazivne štetočine voća D. suzukii na teritoriji Srbije.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae): A new invasive pest in Serbia
T1  - Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae) - nova invazivna vrsta u Srbiji
EP  - 104
IS  - 3
SP  - 99
VL  - 65
DO  - 10.5937/zasbilj1403099T
ER  - 

@article{
author = "Toševski, Ivo and Milenković, Slobodan and Krstić, Oliver and Kosovac, Andrea and Jakovljević, Miljana and Mitrović, Milana and Cvrković, Tatjana and Jović, Jelena",
year = "2014",
abstract = "During October and November 2014, a survey was conducted in order to establish the presence of the invasive pest Drosophila suzukii (Matsumura, 1931) on the territory of Serbia. Survey revealed the presence of this fly in four districts (Rasinski, Mačvanski, Raški and Pčinjski), in sampled raspberry and blackberry ripe fruits, as well as in common fig and grape. Collected fruits were used for the rearing of Drosophilid larvae to adults, which were subsequently subjected to morphological and molecular characterization. The presence of D. suzukii was confirmed in all surveyed districts, as well as in Zemun (City of Belgrade). This is the first report of the highly invasive fruit pest D. suzukii on the territory of Serbia., Tokom oktobra i novembra 2014. godine, sprovedena su istraživanja u cilju utvrđivanja prisustva i rasprostranjenja invazivne štetočine Drosophila suzukii (Matsumura, 1931) na teritoriji Srbije. Prisustvo ove mušice je utvrđeno u okviru četiri okruga (Rasinski, Mačvanski, Raški, Pčinjski), u uzorkovanim plodovima maline, kupine, smokve i grožđa. Iz plodova su odgajene Drozofilidne larve do adulta koji su zatim analizirani morfološki i molekularno. Prisustvo D. suzukii je utvrđeno na svim lokalitetima u okviru četiri okruga, kao i na teritoriji grada Beograda (Zemun). Ovo je prvi nalaz invazivne štetočine voća D. suzukii na teritoriji Srbije.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae): A new invasive pest in Serbia, Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae) - nova invazivna vrsta u Srbiji",
pages = "104-99",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403099T"
}

Toševski, I., Milenković, S., Krstić, O., Kosovac, A., Jakovljević, M., Mitrović, M., Cvrković, T.,& Jović, J.. (2014). Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae): A new invasive pest in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 99-104.
https://doi.org/10.5937/zasbilj1403099T

Toševski I, Milenković S, Krstić O, Kosovac A, Jakovljević M, Mitrović M, Cvrković T, Jović J. Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae): A new invasive pest in Serbia. in Zaštita bilja. 2014;65(3):99-104.
doi:10.5937/zasbilj1403099T .

Toševski, Ivo, Milenković, Slobodan, Krstić, Oliver, Kosovac, Andrea, Jakovljević, Miljana, Mitrović, Milana, Cvrković, Tatjana, Jović, Jelena, "Drosophila suzukii (Matsumura, 1931) (Siptera: Srosophilidae): A new invasive pest in Serbia" in Zaštita bilja, 65, no. 3 (2014):99-104,
https://doi.org/10.5937/zasbilj1403099T . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/326
AB  - Epidemiology of plant diseases caused by phytoplasmas depends on vector's biology and its preference to the host plant. Grapevine yellows (GY) caused by stolbur phytoplasma (16SrXII-A subgroup, 'Candidatus Phytoplasma solani'), during last 15 years became one of the economically most important diseases of the grapevine in Europe. The cixiid plant­hopper Hyalesthes obsoletus Signoret (Hemiptera: Cixiidae) is a principle vector of stolbur phytoplasma and has the main influence on the incidence of the disease and yield loses in the vineyards. Epidemiological cycle of the Bois Noir (BN) is determined by the host plant of the H. obsoletus which is primal source of the disease in the vineyard and it affects presence of the pathogen in the insect's population. Presence of stolbur phytoplasma and symptoms of the BN disease is registered in all wine growing regions in Serbia. This research was focused toward determining host plants of the H. obsoletus and its ge­netic characteristics in order to determine epidemiological characteristics of BN in Serbia. Based on this field research, along with populations associated with previously known host plants nettle (Urtica dioica) and bindweed (Convolvulus arvensis), new host plant association with Crepis foetida L (Asteraceae) was registered on several localities in east and south Serbia. Genetic structure of H. obsoletus populations associated with specific host plants is analyzed in order to obtain precise information about epidemiological cycle of stolbur phyto­plasma in agroecosystems in Serbia. According to results of the three analyzed mtDNA regions, genetic differentiation is present between H. obsoletus populations associated with C. arvensis and U. dioica on the one and C. foetida on the other side. Domination of one of these two genotypes in nature and distribution of the host plants provides the basis for further research of the potential vector ability and characterization of the stolbur phytoplasma which is being transmitted by H. obsoletus and epidemiological cycles related to the new host plant in Serbia.
AB  - Epidemiologija biljnih bolesti uzrokovanih fitoplazmama direktno je zavisna od biologije insekta vektora i njegove preferentnosti prema biljkama domaćinima. Bolest žutila vinove loze Bois Noir (BN) uzrokovana prisustvom stolbur fitoplazme (16SrXII-A podgrupa, 'Candidatus Phytoplasma solani'), tokom zadnjih 15 godina je postala jedna od ekonomski najznačajnijih bolesti vinove loze u Evropi. Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae) kao glavni vektor stolbur fitoplazme na vinovu lozu direktno utiče na pojavu i učestalost ove bolesti, kao i na stepen štete u vinogradima. Epidemiološki ciklus BN fitoplazme uslovljen je biljkom domaćinom cikade H. obsoletus koja predstavlja izvor zaraze u vinogradu i od koje direktno zavisi prisustvo patogena u populaciji insekta. Prisustvo stolbur fitoplazme i simptoma BN bolesti registrovano je u svim vinogradarskim regionima Srbije. U cilju utvrđivanja epidemioloških specifičnosti prenošenja stolbur fitoplazme u Srbiji, istraživanja u ovoj studiji su bila usmerena u pravcu determinisanja biljaka domaćina adulta cikade H. obsoletus i njihove genetičke osobenosti. Primarno su analizirane populacije u asocijaciji sa ranije poznatim domaćinima, koprivom (Urtica dioica L.) i poponcem (Convolvulus arvensis L.). Na osnovu sprovedenih terenskih istraživanja utvrđena je nova asocijacija odraslih jedinki H. obsoletus sa biljkom Crepis foetida L. (Asteraceae) na više lokaliteta u istočnoj i južnoj Srbiji. Genetička struktura populacija cikade Hyalesthes obsoletus vezanih za odgovarajuće biljke domaćine je analizirana radi dobijanja jasne slike epidemioloških ciklusa stolbur fitoplazme u gajenim kulturama u Srbiji. Na osnovu analize tri mitohondrijalna genska regiona utvrđena je genetička diferencijacija populacija H. obsoletus u vidu dva genotipa vezanih svojim životnim ciklusom za C. arvensis i U. dioica sa jedne strane, odnosno za novoutvrđenog domaćina C. foetida sa druge strane. Dominacija jednog od ova dva genotipa u prirodi i distribucija biljaka domaćina daju osnov za dalja istraživanja potencijalne vektorske uloge i karakterizacije stolbur fitoplazme koju H. obsoletus prenosi kao i epidemioloških ciklusa vezanih za novu biljku domaćina u Srbiji.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Crepis foetida L.: New host plant of cixiid planthopper Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vector of stolbur phytoplasma
T1  - Crepis foetida L. - nova biljka domaćin cikade Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vektora stolbur fitoplazme
EP  - 14
IS  - 1
SP  - 7
VL  - 65
DO  - 10.5937/zasbilj1401007K
ER  - 

@article{
author = "Kosovac, Andrea and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2014",
abstract = "Epidemiology of plant diseases caused by phytoplasmas depends on vector's biology and its preference to the host plant. Grapevine yellows (GY) caused by stolbur phytoplasma (16SrXII-A subgroup, 'Candidatus Phytoplasma solani'), during last 15 years became one of the economically most important diseases of the grapevine in Europe. The cixiid plant­hopper Hyalesthes obsoletus Signoret (Hemiptera: Cixiidae) is a principle vector of stolbur phytoplasma and has the main influence on the incidence of the disease and yield loses in the vineyards. Epidemiological cycle of the Bois Noir (BN) is determined by the host plant of the H. obsoletus which is primal source of the disease in the vineyard and it affects presence of the pathogen in the insect's population. Presence of stolbur phytoplasma and symptoms of the BN disease is registered in all wine growing regions in Serbia. This research was focused toward determining host plants of the H. obsoletus and its ge­netic characteristics in order to determine epidemiological characteristics of BN in Serbia. Based on this field research, along with populations associated with previously known host plants nettle (Urtica dioica) and bindweed (Convolvulus arvensis), new host plant association with Crepis foetida L (Asteraceae) was registered on several localities in east and south Serbia. Genetic structure of H. obsoletus populations associated with specific host plants is analyzed in order to obtain precise information about epidemiological cycle of stolbur phyto­plasma in agroecosystems in Serbia. According to results of the three analyzed mtDNA regions, genetic differentiation is present between H. obsoletus populations associated with C. arvensis and U. dioica on the one and C. foetida on the other side. Domination of one of these two genotypes in nature and distribution of the host plants provides the basis for further research of the potential vector ability and characterization of the stolbur phytoplasma which is being transmitted by H. obsoletus and epidemiological cycles related to the new host plant in Serbia., Epidemiologija biljnih bolesti uzrokovanih fitoplazmama direktno je zavisna od biologije insekta vektora i njegove preferentnosti prema biljkama domaćinima. Bolest žutila vinove loze Bois Noir (BN) uzrokovana prisustvom stolbur fitoplazme (16SrXII-A podgrupa, 'Candidatus Phytoplasma solani'), tokom zadnjih 15 godina je postala jedna od ekonomski najznačajnijih bolesti vinove loze u Evropi. Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae) kao glavni vektor stolbur fitoplazme na vinovu lozu direktno utiče na pojavu i učestalost ove bolesti, kao i na stepen štete u vinogradima. Epidemiološki ciklus BN fitoplazme uslovljen je biljkom domaćinom cikade H. obsoletus koja predstavlja izvor zaraze u vinogradu i od koje direktno zavisi prisustvo patogena u populaciji insekta. Prisustvo stolbur fitoplazme i simptoma BN bolesti registrovano je u svim vinogradarskim regionima Srbije. U cilju utvrđivanja epidemioloških specifičnosti prenošenja stolbur fitoplazme u Srbiji, istraživanja u ovoj studiji su bila usmerena u pravcu determinisanja biljaka domaćina adulta cikade H. obsoletus i njihove genetičke osobenosti. Primarno su analizirane populacije u asocijaciji sa ranije poznatim domaćinima, koprivom (Urtica dioica L.) i poponcem (Convolvulus arvensis L.). Na osnovu sprovedenih terenskih istraživanja utvrđena je nova asocijacija odraslih jedinki H. obsoletus sa biljkom Crepis foetida L. (Asteraceae) na više lokaliteta u istočnoj i južnoj Srbiji. Genetička struktura populacija cikade Hyalesthes obsoletus vezanih za odgovarajuće biljke domaćine je analizirana radi dobijanja jasne slike epidemioloških ciklusa stolbur fitoplazme u gajenim kulturama u Srbiji. Na osnovu analize tri mitohondrijalna genska regiona utvrđena je genetička diferencijacija populacija H. obsoletus u vidu dva genotipa vezanih svojim životnim ciklusom za C. arvensis i U. dioica sa jedne strane, odnosno za novoutvrđenog domaćina C. foetida sa druge strane. Dominacija jednog od ova dva genotipa u prirodi i distribucija biljaka domaćina daju osnov za dalja istraživanja potencijalne vektorske uloge i karakterizacije stolbur fitoplazme koju H. obsoletus prenosi kao i epidemioloških ciklusa vezanih za novu biljku domaćina u Srbiji.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Crepis foetida L.: New host plant of cixiid planthopper Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vector of stolbur phytoplasma, Crepis foetida L. - nova biljka domaćin cikade Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vektora stolbur fitoplazme",
pages = "14-7",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401007K"
}

Kosovac, A., Jakovljević, M., Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2014). Crepis foetida L.: New host plant of cixiid planthopper Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vector of stolbur phytoplasma. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 7-14.
https://doi.org/10.5937/zasbilj1401007K

Kosovac A, Jakovljević M, Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Crepis foetida L.: New host plant of cixiid planthopper Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vector of stolbur phytoplasma. in Zaštita bilja. 2014;65(1):7-14.
doi:10.5937/zasbilj1401007K .

Kosovac, Andrea, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Crepis foetida L.: New host plant of cixiid planthopper Hyalesthes obsoletus Signoret 1865 (Hemiptera: Cixiidae), vector of stolbur phytoplasma" in Zaštita bilja, 65, no. 1 (2014):7-14,
https://doi.org/10.5937/zasbilj1401007K . .

TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/262
AB  - Diversity of the cicads from the subfamily Deltocephalinae have been investigated in Serbia, over a period of 2004-2010 in vineyards on localities Vršac, Topola, Rajac and Jasenovik and maize fields of South Banat. Individuals of different species belonging to a subfamily Deltocephalinae were collected in plantations and crops, as well as from a surrounding vegetation. The most abundant species on all localities was Psammotettix alienus (Dahlbom, 1850). Less numerous species of the subfamily were Euscelis incisus (Kirschbaum, 1858), Neoaliturus fenestratus (Herrich-Schäffer, 1834) and Errastunus ocellaris (Fallén, 1806), while the percentage representation of Doratura impudica Horváth, 1897 was much lower. Among the representatives of the subfamily Deltocephalinae, species that have been recorded in the South Banat were Arocephalus languidus (Flor, 1861), Euscelis distinguendus (Kirschbaum, 1858) and Metalimnus steini (Fieber, 1869). In vineyards of Jasenovik as the most dominant species occurred N. fenestratus.
AB  - Istraživanje diverziteta cikada podfamilije Deltocephalinae sprovedeno je u periodu od 2004. do 2010. godine u vinogradima na lokalitetima Vršac, Topola, Rajac i Jasenovik i usevima kukuruza u južnom Banatu. Jedinke različitih vrsta podfamilije Deltocephalinae su sakupljane u zasadima i usevima, kao i na okolnoj vegetaciji. Najbrojnija vrsta na svima lokalitetima bila je Psammotettix alienus (Dahlbom, 1850). Manje brojne vrste ove podfamilije bile su vrste Euscelis incisus (Kirschbaum, 1858), Neoaliturus fenestratus (Herrich-Schäffer, 1834) i Errastunus ocellaris (Fallén, 1806), dok je procentualna zastupljenost vrste Doratura impudica Horváth, 1897 bila znatno manja. Od predstavnika podfamilije Deltocephalinae, vrste koje su zabeležene u južnom Banatu su Arocephalus languidus (Flor, 1861), Euscelis distinguendus (Kirschbaum, 1858) i Metalimnus steini (Fieber, 1869). U vinogradima Jasenovika kao najdominantnija vrsta javljala se vrsta N. fenestratus.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Diversity of Auchenorrhyncha species of subfamily Deltocephalinae in Serbian agroecosystems and potential phytoplasma vectors
T1  - Diverzitet faune cikada podfamilije Deltocephalinae u agroekosistemima Srbije i potencijalni vektori fitoplazmi
EP  - 143
IS  - 3
SP  - 134
VL  - 64
ER  - 

@article{
author = "Jakovljević, Miljana and Kosovac, Andrea and Krstić, Oliver and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Cvrković, Tatjana",
year = "2013",
abstract = "Diversity of the cicads from the subfamily Deltocephalinae have been investigated in Serbia, over a period of 2004-2010 in vineyards on localities Vršac, Topola, Rajac and Jasenovik and maize fields of South Banat. Individuals of different species belonging to a subfamily Deltocephalinae were collected in plantations and crops, as well as from a surrounding vegetation. The most abundant species on all localities was Psammotettix alienus (Dahlbom, 1850). Less numerous species of the subfamily were Euscelis incisus (Kirschbaum, 1858), Neoaliturus fenestratus (Herrich-Schäffer, 1834) and Errastunus ocellaris (Fallén, 1806), while the percentage representation of Doratura impudica Horváth, 1897 was much lower. Among the representatives of the subfamily Deltocephalinae, species that have been recorded in the South Banat were Arocephalus languidus (Flor, 1861), Euscelis distinguendus (Kirschbaum, 1858) and Metalimnus steini (Fieber, 1869). In vineyards of Jasenovik as the most dominant species occurred N. fenestratus., Istraživanje diverziteta cikada podfamilije Deltocephalinae sprovedeno je u periodu od 2004. do 2010. godine u vinogradima na lokalitetima Vršac, Topola, Rajac i Jasenovik i usevima kukuruza u južnom Banatu. Jedinke različitih vrsta podfamilije Deltocephalinae su sakupljane u zasadima i usevima, kao i na okolnoj vegetaciji. Najbrojnija vrsta na svima lokalitetima bila je Psammotettix alienus (Dahlbom, 1850). Manje brojne vrste ove podfamilije bile su vrste Euscelis incisus (Kirschbaum, 1858), Neoaliturus fenestratus (Herrich-Schäffer, 1834) i Errastunus ocellaris (Fallén, 1806), dok je procentualna zastupljenost vrste Doratura impudica Horváth, 1897 bila znatno manja. Od predstavnika podfamilije Deltocephalinae, vrste koje su zabeležene u južnom Banatu su Arocephalus languidus (Flor, 1861), Euscelis distinguendus (Kirschbaum, 1858) i Metalimnus steini (Fieber, 1869). U vinogradima Jasenovika kao najdominantnija vrsta javljala se vrsta N. fenestratus.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Diversity of Auchenorrhyncha species of subfamily Deltocephalinae in Serbian agroecosystems and potential phytoplasma vectors, Diverzitet faune cikada podfamilije Deltocephalinae u agroekosistemima Srbije i potencijalni vektori fitoplazmi",
pages = "143-134",
number = "3",
volume = "64"
}

Jakovljević, M., Kosovac, A., Krstić, O., Mitrović, M., Jović, J., Toševski, I.,& Cvrković, T.. (2013). Diversity of Auchenorrhyncha species of subfamily Deltocephalinae in Serbian agroecosystems and potential phytoplasma vectors. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(3), 134-143.

Jakovljević M, Kosovac A, Krstić O, Mitrović M, Jović J, Toševski I, Cvrković T. Diversity of Auchenorrhyncha species of subfamily Deltocephalinae in Serbian agroecosystems and potential phytoplasma vectors. in Zaštita bilja. 2013;64(3):134-143..

Jakovljević, Miljana, Kosovac, Andrea, Krstić, Oliver, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Cvrković, Tatjana, "Diversity of Auchenorrhyncha species of subfamily Deltocephalinae in Serbian agroecosystems and potential phytoplasma vectors" in Zaštita bilja, 64, no. 3 (2013):134-143.

TY  - JOUR
AU  - Krstić, Oliver
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Kosovac, Andrea
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/247
AB  - The investigation of faunal diversity of Auchenorryncha of Montenegro was conducted in vineyards at six localities. Insects were collected using sweep nets and mouth-aspirators from grapevine at localities Martinići, Beri, Drušići, Šušunja, Godinje and Nudo. A total number of collected specimens was 3579, belonging to 10 families, 32 genera and 39 species. The most numerous was family Cicadellidae with 22 species, followed by Cixidae (4), Issidae (3), Cercopidae, Aphrophoridae and Tettigometridae with two species and Delphacidae, Dictyopharidae, Flatidae and Membracidae with one species recorded. The most abundant were species belonging to the families Cercopidae, Cicadellidae, Dictyopharidae and Cixiidae. Confirmed vector of BN phytoplasma, H. obsoletus was highly abundant, while the presence of potential vector, D. europaea was also recorded.
AB  - Istraživanje diverziteta faune Auchenorryncha u Crnoj Gori sprovedeno je u vinogradima na 6 lokaliteta. Cikade su sakupljane metodom košenja tokom 2010. i 2011. godine u vinogradima na lokalitetima Martinići, Beri, Drušići, Šušunja, Godinje i Nudo. Ukupno je sakupljeno 3579 primeraka među kojima je identifikovano 39 vrsta iz 32 roda i 10 familja. Familija Cicadellidae je bila zastupljena sa najvećim brojem vrsta (22), fam. Cixiidae sa 4 vrste, a fam. Issidae sa 3 vrste. Sa po 2 vrste bile su zastupljene fam. Cercopidae, Aphrophoridae i Tettigometridae, dok su fam. Delphacidae, Dictyopharidae, Flatidae i Membracidae bile zastupljene sa po jednom vrstom. U najvećoj brojnosti prisutne su vrste iz familija Cercopidae, Cicadellidae, Dictyopharidae i Cixiidae. Od potvrđenih vektora Bois noir fitoplazme u visokoj brojnosti prisutna je vrsta Hyalesthes obsoletus, dok je od potencijalnih vektora utvrđeno prisustvo vrste Dictyophara europaea.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Diversity of the Auchenorrhyncha fauna in vineyards of Montenegro
T1  - Diverzitet faune Auchenorrhyncha u vinogradima Crne Gore
EP  - 113
IS  - 2
SP  - 108
VL  - 63
ER  - 

@article{
author = "Krstić, Oliver and Radonjić, Sanja and Hrnčić, Snježana and Cvrković, Tatjana and Mitrović, Milana and Kosovac, Andrea and Toševski, Ivo and Jović, Jelena",
year = "2012",
abstract = "The investigation of faunal diversity of Auchenorryncha of Montenegro was conducted in vineyards at six localities. Insects were collected using sweep nets and mouth-aspirators from grapevine at localities Martinići, Beri, Drušići, Šušunja, Godinje and Nudo. A total number of collected specimens was 3579, belonging to 10 families, 32 genera and 39 species. The most numerous was family Cicadellidae with 22 species, followed by Cixidae (4), Issidae (3), Cercopidae, Aphrophoridae and Tettigometridae with two species and Delphacidae, Dictyopharidae, Flatidae and Membracidae with one species recorded. The most abundant were species belonging to the families Cercopidae, Cicadellidae, Dictyopharidae and Cixiidae. Confirmed vector of BN phytoplasma, H. obsoletus was highly abundant, while the presence of potential vector, D. europaea was also recorded., Istraživanje diverziteta faune Auchenorryncha u Crnoj Gori sprovedeno je u vinogradima na 6 lokaliteta. Cikade su sakupljane metodom košenja tokom 2010. i 2011. godine u vinogradima na lokalitetima Martinići, Beri, Drušići, Šušunja, Godinje i Nudo. Ukupno je sakupljeno 3579 primeraka među kojima je identifikovano 39 vrsta iz 32 roda i 10 familja. Familija Cicadellidae je bila zastupljena sa najvećim brojem vrsta (22), fam. Cixiidae sa 4 vrste, a fam. Issidae sa 3 vrste. Sa po 2 vrste bile su zastupljene fam. Cercopidae, Aphrophoridae i Tettigometridae, dok su fam. Delphacidae, Dictyopharidae, Flatidae i Membracidae bile zastupljene sa po jednom vrstom. U najvećoj brojnosti prisutne su vrste iz familija Cercopidae, Cicadellidae, Dictyopharidae i Cixiidae. Od potvrđenih vektora Bois noir fitoplazme u visokoj brojnosti prisutna je vrsta Hyalesthes obsoletus, dok je od potencijalnih vektora utvrđeno prisustvo vrste Dictyophara europaea.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Diversity of the Auchenorrhyncha fauna in vineyards of Montenegro, Diverzitet faune Auchenorrhyncha u vinogradima Crne Gore",
pages = "113-108",
number = "2",
volume = "63"
}

Krstić, O., Radonjić, S., Hrnčić, S., Cvrković, T., Mitrović, M., Kosovac, A., Toševski, I.,& Jović, J.. (2012). Diversity of the Auchenorrhyncha fauna in vineyards of Montenegro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(2), 108-113.

Krstić O, Radonjić S, Hrnčić S, Cvrković T, Mitrović M, Kosovac A, Toševski I, Jović J. Diversity of the Auchenorrhyncha fauna in vineyards of Montenegro. in Zaštita bilja. 2012;63(2):108-113..

Krstić, Oliver, Radonjić, Sanja, Hrnčić, Snježana, Cvrković, Tatjana, Mitrović, Milana, Kosovac, Andrea, Toševski, Ivo, Jović, Jelena, "Diversity of the Auchenorrhyncha fauna in vineyards of Montenegro" in Zaštita bilja, 63, no. 2 (2012):108-113.