TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milijašević, Svetlana
AU  - Todorović, Biljana
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/84
AB  - A large-scale outbreak of fire blight symptoms caused by Erwinia amylovora was recorded in pome fruit trees during 2007. In addition to fruit necrosis and shoot blight as the typical disease symptoms, dark purple necrosis was observed in the root collar area girdling the trunk just above the ground and thus withering the whole apple tree. Since similar symptoms on apple trees could be caused by E. amylovora or one of several phytopathogenic fungi of the genera Phomopsis and Phytophthora, an investigation was conducted to identify the causal agent of this disease. Levan-producing, nonfluorescent bacteria were isolated from diseased samples. The isolated strains produced HR in tobacco leaves and necrosis of artificially inoculated, immature pear fruits, followed by oozing of bacterial exudate, a characterisitic of E. amylovora. Based on the results of pathogenicity tests, biochemical characteristics, ELISA test and PCR analysis, it was confirmed that the investigated strains belonged to E. amylovora, causing the root collar necrosis of apple trees as an atypical symptom of this bacterium in Serbia. The explanation of this symptom may be that the vegetative rootstocks were infected with E. amylovora. Therefore, the development of diagnostic protocols for detection of E. amylovora in apple rootstock is very important for health inspections of planting materials.
AB  - Tokom 2007. godine zabeležena je masovna pojava bakteriozne plamenjače jabučastih voćaka prouzrokovana bakterijom Erwinia amylovora. Pored nekroze plodova i plamenjače mladara, tipičnih simptoma bolesti, našu pažnju je privukla pojava nekroze mrkoljubičaste boje u zoni korenovog vrata, koja prstenasto zahvata prizemni deo i prouzrokuje izumiranje stabala jabuke. S obzirom na to da uzrok ovakvih patoloških promena može da bude E. amylovora, ali i fitopatogene gljive iz rodova Phomopsis i Phytophthora, preduzeta su istraživanja sa ciljem da se utvrdi etiologija ovog oboljenja jabuke. Iz obolelih uzoraka izolovane su bakterije koje stvaraju levan, ali ne i fluorescentni pigment. Proučavani sojevi prouzrokovali su HR na listovima duvana i nekrozu inokulisanih, nesazrelih plodova kruške, uz obilno stvaranje bakterijskog eksudata, što je karakterističan znak E. amylovora. Na osnovu rezultata dobijenih pri testovima provere patogenosti, ali i rezultata biohemijskih odlika, ELISA testa i molekularnih metoda (PCR), potvrđeno je da proučavani izolati pripadaju bakteriji E. amylovora, koja je uzročnik simptoma nekroze korenovog vrata stabala jabuke, prvi put zabeleženih u Srbiji. Moguće objašnjenje ove pojave je da su vegetativne podloge za kalemljenje bile inficirane ovom bakterijom. Zbog toga je veoma bitno razvijati dijagnostičke protokole za utvrđivanje prisustva E. amylovora u podlogama za kalemljenje jabuke, tokom procesa kontrole zdravstvene ispravnosti sadnog materijala.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Erwinia amylovora: The causal agent of root collar necrosis of apple trees
T1  - Erwinia amylovora - prouzrokovač nekroze korenovog vrata stabla jabuke
EP  - 23
IS  - 1
SP  - 17
VL  - 23
ER  - 

@article{
author = "Gavrilović, Veljko and Milijašević, Svetlana and Todorović, Biljana and Živković, Svetlana and Trkulja, Nenad",
year = "2008",
abstract = "A large-scale outbreak of fire blight symptoms caused by Erwinia amylovora was recorded in pome fruit trees during 2007. In addition to fruit necrosis and shoot blight as the typical disease symptoms, dark purple necrosis was observed in the root collar area girdling the trunk just above the ground and thus withering the whole apple tree. Since similar symptoms on apple trees could be caused by E. amylovora or one of several phytopathogenic fungi of the genera Phomopsis and Phytophthora, an investigation was conducted to identify the causal agent of this disease. Levan-producing, nonfluorescent bacteria were isolated from diseased samples. The isolated strains produced HR in tobacco leaves and necrosis of artificially inoculated, immature pear fruits, followed by oozing of bacterial exudate, a characterisitic of E. amylovora. Based on the results of pathogenicity tests, biochemical characteristics, ELISA test and PCR analysis, it was confirmed that the investigated strains belonged to E. amylovora, causing the root collar necrosis of apple trees as an atypical symptom of this bacterium in Serbia. The explanation of this symptom may be that the vegetative rootstocks were infected with E. amylovora. Therefore, the development of diagnostic protocols for detection of E. amylovora in apple rootstock is very important for health inspections of planting materials., Tokom 2007. godine zabeležena je masovna pojava bakteriozne plamenjače jabučastih voćaka prouzrokovana bakterijom Erwinia amylovora. Pored nekroze plodova i plamenjače mladara, tipičnih simptoma bolesti, našu pažnju je privukla pojava nekroze mrkoljubičaste boje u zoni korenovog vrata, koja prstenasto zahvata prizemni deo i prouzrokuje izumiranje stabala jabuke. S obzirom na to da uzrok ovakvih patoloških promena može da bude E. amylovora, ali i fitopatogene gljive iz rodova Phomopsis i Phytophthora, preduzeta su istraživanja sa ciljem da se utvrdi etiologija ovog oboljenja jabuke. Iz obolelih uzoraka izolovane su bakterije koje stvaraju levan, ali ne i fluorescentni pigment. Proučavani sojevi prouzrokovali su HR na listovima duvana i nekrozu inokulisanih, nesazrelih plodova kruške, uz obilno stvaranje bakterijskog eksudata, što je karakterističan znak E. amylovora. Na osnovu rezultata dobijenih pri testovima provere patogenosti, ali i rezultata biohemijskih odlika, ELISA testa i molekularnih metoda (PCR), potvrđeno je da proučavani izolati pripadaju bakteriji E. amylovora, koja je uzročnik simptoma nekroze korenovog vrata stabala jabuke, prvi put zabeleženih u Srbiji. Moguće objašnjenje ove pojave je da su vegetativne podloge za kalemljenje bile inficirane ovom bakterijom. Zbog toga je veoma bitno razvijati dijagnostičke protokole za utvrđivanje prisustva E. amylovora u podlogama za kalemljenje jabuke, tokom procesa kontrole zdravstvene ispravnosti sadnog materijala.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Erwinia amylovora: The causal agent of root collar necrosis of apple trees, Erwinia amylovora - prouzrokovač nekroze korenovog vrata stabla jabuke",
pages = "23-17",
number = "1",
volume = "23"
}

Gavrilović, V., Milijašević, S., Todorović, B., Živković, S.,& Trkulja, N.. (2008). Erwinia amylovora: The causal agent of root collar necrosis of apple trees. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 23(1), 17-23.

Gavrilović V, Milijašević S, Todorović B, Živković S, Trkulja N. Erwinia amylovora: The causal agent of root collar necrosis of apple trees. in Pesticidi i fitomedicina. 2008;23(1):17-23..

Gavrilović, Veljko, Milijašević, Svetlana, Todorović, Biljana, Živković, Svetlana, Trkulja, Nenad, "Erwinia amylovora: The causal agent of root collar necrosis of apple trees" in Pesticidi i fitomedicina, 23, no. 1 (2008):17-23.

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Obradović, Aleksa
AU  - Milijašević, Svetlana
AU  - Živković, Svetlana
AU  - Arsenijević, M.
AU  - Vojinović, Milić
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/83
AB  - In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanotneles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25 degrees C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - Proceedings of the Eleventh International Workshop on Fire Blight
T1  - Sorbus sp - New host of Erwinia amylovora in Serbia
SP  - 351
VL  - 793
ER  - 

@conference{
author = "Gavrilović, Veljko and Obradović, Aleksa and Milijašević, Svetlana and Živković, Svetlana and Arsenijević, M. and Vojinović, Milić",
year = "2008",
abstract = "In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanotneles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25 degrees C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "Proceedings of the Eleventh International Workshop on Fire Blight",
title = "Sorbus sp - New host of Erwinia amylovora in Serbia",
pages = "351",
volume = "793"
}

Gavrilović, V., Obradović, A., Milijašević, S., Živković, S., Arsenijević, M.,& Vojinović, M.. (2008). Sorbus sp - New host of Erwinia amylovora in Serbia. in Proceedings of the Eleventh International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 793, 351.

Gavrilović V, Obradović A, Milijašević S, Živković S, Arsenijević M, Vojinović M. Sorbus sp - New host of Erwinia amylovora in Serbia. in Proceedings of the Eleventh International Workshop on Fire Blight. 2008;793:351..

Gavrilović, Veljko, Obradović, Aleksa, Milijašević, Svetlana, Živković, Svetlana, Arsenijević, M., Vojinović, Milić, "Sorbus sp - New host of Erwinia amylovora in Serbia" in Proceedings of the Eleventh International Workshop on Fire Blight, 793 (2008):351.

TY  - JOUR
AU  - Milijašević, Svetlana
AU  - Gavrilović, Veljko
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Pulawska, Joanna
PY  - 2007
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/53
AB  - During the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemical and physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 gene located on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia.
AB  - Tokom proleća 2003. godine, zapaženi su tumori na korenu i korenovom vratu mladih biljaka maline sorte Vilamette u okolini Valjeva. Iz obolelih uzoraka izolovane su fitopatogene bakterije. Proučavanjem patogenih, morfoloških i diferencijalnih biohemijsko-fizioloških karakteristika izolovanih sojeva, utvrđeno je da proučavani sojevi pripadaju bakteriji Agrobacterium radiobacter (biovar 1). U cilju potvrde identiteta izolovanih sojeva lančanom reakcijom polimeraze (PCR), korišćeni su prajmeri komplementarni tms2 genu lociranom na Ti plazmidu. U prvoj PCR reakciji izvedenoj korišćenjem tms2F1 + tms2R2 para prajmera, detektovani su fragmenti nukleinske kiseline veličine 617 baznih parova (bp), specifični za tumorogene sojeve A. tumefaciens. U drugoj PCR reakciji korišćenjem para prajmera tms2F1 i tms2B su amplifikovani PCR produkti očekivane veličine 458 bp, karakteristični za ovu bakteriju. Na osnovu multiplex PCR reakcije korišćenjem prajmera komplementarnih na hromozomalni gen koji kodira 23S rRNA sojevi izolovani iz tumora na korenu maline svrstani su u biovar 1 Agrobacterium (A. radiobacter). Ovo je prvi nalaz ove bakterije na malini u Srbiji.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - First report of tumorigenic Agrobacterium radiobacter on raspberry in Serbia
T1  - Prvi nalaz tumorogenih sojeva Agrobacterium radiobacter na malini u Srbiji
EP  - 119
IS  - 2
SP  - 113
VL  - 22
ER  - 

@article{
author = "Milijašević, Svetlana and Gavrilović, Veljko and Živković, Svetlana and Trkulja, Nenad and Pulawska, Joanna",
year = "2007",
abstract = "During the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemical and physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 gene located on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia., Tokom proleća 2003. godine, zapaženi su tumori na korenu i korenovom vratu mladih biljaka maline sorte Vilamette u okolini Valjeva. Iz obolelih uzoraka izolovane su fitopatogene bakterije. Proučavanjem patogenih, morfoloških i diferencijalnih biohemijsko-fizioloških karakteristika izolovanih sojeva, utvrđeno je da proučavani sojevi pripadaju bakteriji Agrobacterium radiobacter (biovar 1). U cilju potvrde identiteta izolovanih sojeva lančanom reakcijom polimeraze (PCR), korišćeni su prajmeri komplementarni tms2 genu lociranom na Ti plazmidu. U prvoj PCR reakciji izvedenoj korišćenjem tms2F1 + tms2R2 para prajmera, detektovani su fragmenti nukleinske kiseline veličine 617 baznih parova (bp), specifični za tumorogene sojeve A. tumefaciens. U drugoj PCR reakciji korišćenjem para prajmera tms2F1 i tms2B su amplifikovani PCR produkti očekivane veličine 458 bp, karakteristični za ovu bakteriju. Na osnovu multiplex PCR reakcije korišćenjem prajmera komplementarnih na hromozomalni gen koji kodira 23S rRNA sojevi izolovani iz tumora na korenu maline svrstani su u biovar 1 Agrobacterium (A. radiobacter). Ovo je prvi nalaz ove bakterije na malini u Srbiji.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "First report of tumorigenic Agrobacterium radiobacter on raspberry in Serbia, Prvi nalaz tumorogenih sojeva Agrobacterium radiobacter na malini u Srbiji",
pages = "119-113",
number = "2",
volume = "22"
}

Milijašević, S., Gavrilović, V., Živković, S., Trkulja, N.,& Pulawska, J.. (2007). First report of tumorigenic Agrobacterium radiobacter on raspberry in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 22(2), 113-119.

Milijašević S, Gavrilović V, Živković S, Trkulja N, Pulawska J. First report of tumorigenic Agrobacterium radiobacter on raspberry in Serbia. in Pesticidi i fitomedicina. 2007;22(2):113-119..

Milijašević, Svetlana, Gavrilović, Veljko, Živković, Svetlana, Trkulja, Nenad, Pulawska, Joanna, "First report of tumorigenic Agrobacterium radiobacter on raspberry in Serbia" in Pesticidi i fitomedicina, 22, no. 2 (2007):113-119.