TY  - JOUR
AU  - Jošić, Dragana
AU  - Pivić, Radmila
AU  - Miladinović, Miroslav
AU  - Starović, Mira
AU  - Pavlović, Snežana
AU  - Đurić, Simonida
AU  - Jarak, Mirjana
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/206
AB  - Antibiotic production by plant-associated microorganisms represents an environmentally compatible method of disease control in agriculture. However, a vide application of bacterial strains needs careful selection and genetic characterization. In this investigation, selected Pseudomonas strains were characterized by rep-PCR methods using ERIC and (GTG)5 primers, and partial 16S rDNA sequence analysis. None of strains produced homoserine lactones (C4, C6, C8) as quorum sensing signal molecules. Very poor production of phenazines and no significant fungal inhibition was observed for PS4 and PS6 strains. High amount of phenazines were produced by Pseudomonas sp. strain PS2, which inhibited mycelial growth of 10 phytopatogenic fungi in percent of 25 (Verticillium sp.) to 65 (Fusarium equiseti). Genetic characterization of the Pseudomonas sp. PS2 and evaluation of phenazines production, as the main trait for growth inhibition of phytopathogenic fungi, will allow its application as a biosafe PGPR for field experiments of plant disease control.
AB  - Ekološki prihvatljiv metod kontrole bolesti bilja u poljoprivredi obuhvata primenu rizosfernih mikroorganizama koji produkuju antibiotike. Široka primena bakterija zahteva njihovu pažljivu selekciju i genetičku karakterizaciju. Ova istraživanja obuhvatila su karakterizaciju selektovanih Pseudomonas sojeva na osnovu rep-PCR metode korišćenjem ERIC i (GTG)5 prajmera, kao i parcijalne sekvence 16S rDNA. Nije detektovana produkcija acil-homoserin laktona (C4, C6, C8) kao QS molekula. Sojevi PS4 i PS6 su vrlo oskudno produkovali fenazine i nisu značajno inhibirali rast fitopatogenih gljiva. Soj Pseudomonas sp. PS2 je obilno produkovao 2-OH-PCA i PCA i inhibirao rast micelija 10 fitopatogenih gljiva od 25% (Verticillium sp.) do 65% (Fusarium equiseti). Genetička karakterizacija soja Pseudomonas sp. PS2 i detekcija produkcije fenazina kao glavnog inhibitora rasta fitopatogenih gljiva omogućiće primenu ovog soja kao bezbednog PGP agensa za biološku kontrolu u budućim ogledima u polju.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina
T1  - Antifungalna aktivnost i genetički diverzitet selektovanih Pseudomonas spp. iz rizosfere kukuruza u Vojvodini
EP  - 388
IS  - 2
SP  - 377
VL  - 44
DO  - 10.2298/GENSR1202377J
ER  - 

@article{
author = "Jošić, Dragana and Pivić, Radmila and Miladinović, Miroslav and Starović, Mira and Pavlović, Snežana and Đurić, Simonida and Jarak, Mirjana",
year = "2012",
abstract = "Antibiotic production by plant-associated microorganisms represents an environmentally compatible method of disease control in agriculture. However, a vide application of bacterial strains needs careful selection and genetic characterization. In this investigation, selected Pseudomonas strains were characterized by rep-PCR methods using ERIC and (GTG)5 primers, and partial 16S rDNA sequence analysis. None of strains produced homoserine lactones (C4, C6, C8) as quorum sensing signal molecules. Very poor production of phenazines and no significant fungal inhibition was observed for PS4 and PS6 strains. High amount of phenazines were produced by Pseudomonas sp. strain PS2, which inhibited mycelial growth of 10 phytopatogenic fungi in percent of 25 (Verticillium sp.) to 65 (Fusarium equiseti). Genetic characterization of the Pseudomonas sp. PS2 and evaluation of phenazines production, as the main trait for growth inhibition of phytopathogenic fungi, will allow its application as a biosafe PGPR for field experiments of plant disease control., Ekološki prihvatljiv metod kontrole bolesti bilja u poljoprivredi obuhvata primenu rizosfernih mikroorganizama koji produkuju antibiotike. Široka primena bakterija zahteva njihovu pažljivu selekciju i genetičku karakterizaciju. Ova istraživanja obuhvatila su karakterizaciju selektovanih Pseudomonas sojeva na osnovu rep-PCR metode korišćenjem ERIC i (GTG)5 prajmera, kao i parcijalne sekvence 16S rDNA. Nije detektovana produkcija acil-homoserin laktona (C4, C6, C8) kao QS molekula. Sojevi PS4 i PS6 su vrlo oskudno produkovali fenazine i nisu značajno inhibirali rast fitopatogenih gljiva. Soj Pseudomonas sp. PS2 je obilno produkovao 2-OH-PCA i PCA i inhibirao rast micelija 10 fitopatogenih gljiva od 25% (Verticillium sp.) do 65% (Fusarium equiseti). Genetička karakterizacija soja Pseudomonas sp. PS2 i detekcija produkcije fenazina kao glavnog inhibitora rasta fitopatogenih gljiva omogućiće primenu ovog soja kao bezbednog PGP agensa za biološku kontrolu u budućim ogledima u polju.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina, Antifungalna aktivnost i genetički diverzitet selektovanih Pseudomonas spp. iz rizosfere kukuruza u Vojvodini",
pages = "388-377",
number = "2",
volume = "44",
doi = "10.2298/GENSR1202377J"
}

Jošić, D., Pivić, R., Miladinović, M., Starović, M., Pavlović, S., Đurić, S.,& Jarak, M.. (2012). Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina. in Genetika-Belgrade
Serbian Genetics Society., 44(2), 377-388.
https://doi.org/10.2298/GENSR1202377J

Jošić D, Pivić R, Miladinović M, Starović M, Pavlović S, Đurić S, Jarak M. Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina. in Genetika-Belgrade. 2012;44(2):377-388.
doi:10.2298/GENSR1202377J .

Jošić, Dragana, Pivić, Radmila, Miladinović, Miroslav, Starović, Mira, Pavlović, Snežana, Đurić, Simonida, Jarak, Mirjana, "Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina" in Genetika-Belgrade, 44, no. 2 (2012):377-388,
https://doi.org/10.2298/GENSR1202377J . .

TY  - JOUR
AU  - Jošić, Dragana
AU  - Protolipac, Katarina
AU  - Starović, Mira
AU  - Stojanović, Saša
AU  - Pavlović, Snežana
AU  - Miladinović, Miroslav
AU  - Radović, Svetlana
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/226
AB  - Phenazines, secondary metabolites of fluorescent Pseudomonas, represent a group of heterocyclic nitrogen-containing compounds showing a broad spectrum of antibiotic properties. Phenazines producing fluorescent Pseudomonas species are studied extensively for their application in plant disease management. In this study, we examined the antifungal activity of different indigenous Pseudomonas isolates (Q16, B25 and PS2) against the phytopathogenic fungus Alternaria tenuissima, which had infected cardoon (Cynara cardunculus L., Asteraceae). An in vitro experiment demonstrated the antifungal activity of selected indigenous isolates. In addition, an in vivo experiment under gnotobiotic conditions showed suppression of C. cardunculus disease caused by A. tenuissima. The quantification of phenazines revealed significant amounts of phenazine-1-carboxylic acid (PCA) and 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA). PCR analysis confirmed the presence of PCA genes in all examined indigenous Pseudomonas isolates. Based on our results, we assume that these Pseudomonas isolates have potential in controlling plant diseases caused by A. tenuissima.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - Phenazines producing pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon
EP  - 1503
IS  - 4
SP  - 1495
VL  - 64
DO  - 10.2298/ABS1204495J
ER  - 

@article{
author = "Jošić, Dragana and Protolipac, Katarina and Starović, Mira and Stojanović, Saša and Pavlović, Snežana and Miladinović, Miroslav and Radović, Svetlana",
year = "2012",
abstract = "Phenazines, secondary metabolites of fluorescent Pseudomonas, represent a group of heterocyclic nitrogen-containing compounds showing a broad spectrum of antibiotic properties. Phenazines producing fluorescent Pseudomonas species are studied extensively for their application in plant disease management. In this study, we examined the antifungal activity of different indigenous Pseudomonas isolates (Q16, B25 and PS2) against the phytopathogenic fungus Alternaria tenuissima, which had infected cardoon (Cynara cardunculus L., Asteraceae). An in vitro experiment demonstrated the antifungal activity of selected indigenous isolates. In addition, an in vivo experiment under gnotobiotic conditions showed suppression of C. cardunculus disease caused by A. tenuissima. The quantification of phenazines revealed significant amounts of phenazine-1-carboxylic acid (PCA) and 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA). PCR analysis confirmed the presence of PCA genes in all examined indigenous Pseudomonas isolates. Based on our results, we assume that these Pseudomonas isolates have potential in controlling plant diseases caused by A. tenuissima.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Phenazines producing pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon",
pages = "1503-1495",
number = "4",
volume = "64",
doi = "10.2298/ABS1204495J"
}

Jošić, D., Protolipac, K., Starović, M., Stojanović, S., Pavlović, S., Miladinović, M.,& Radović, S.. (2012). Phenazines producing pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 64(4), 1495-1503.
https://doi.org/10.2298/ABS1204495J

Jošić D, Protolipac K, Starović M, Stojanović S, Pavlović S, Miladinović M, Radović S. Phenazines producing pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon. in Archives of Biological Sciences. 2012;64(4):1495-1503.
doi:10.2298/ABS1204495J .

Jošić, Dragana, Protolipac, Katarina, Starović, Mira, Stojanović, Saša, Pavlović, Snežana, Miladinović, Miroslav, Radović, Svetlana, "Phenazines producing pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon" in Archives of Biological Sciences, 64, no. 4 (2012):1495-1503,
https://doi.org/10.2298/ABS1204495J . .