Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Stojanović, M., Popović, V.,& Ivanović, Ž.. (2020). First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease, 104(6), 1864-1864.
https://doi.org/10.1094/PDIS-10-19-2218-PDN

Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Stojanović M, Popović V, Ivanović Ž. First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease. 2020;104(6):1864-1864.
doi:10.1094/PDIS-10-19-2218-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Stojanović, Milan, Popović, Vera, Ivanović, Žarko, "First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia" in Plant Disease, 104, no. 6 (2020):1864-1864,
https://doi.org/10.1094/PDIS-10-19-2218-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/608
AB  - Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.
AB  - Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L
T1  - Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L
IS  - 137
SP  - 49
DO  - 10.2298/ZMSPN1937049I
ER  - 

@article{
author = "Ignjatov, Maja and Vlajić, Slobodan and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2019",
abstract = "Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves., Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L, Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L",
number = "137",
pages = "49",
doi = "10.2298/ZMSPN1937049I"
}

Ignjatov, M., Vlajić, S., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2019). Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(137), 49.
https://doi.org/10.2298/ZMSPN1937049I

Ignjatov M, Vlajić S, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke. 2019;(137):49.
doi:10.2298/ZMSPN1937049I .

Ignjatov, Maja, Vlajić, Slobodan, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L" in Zbornik Matice srpske za prirodne nauke, no. 137 (2019):49,
https://doi.org/10.2298/ZMSPN1937049I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, Dragana
AU  - Ivanović, Žarko
AU  - Karaman, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/541
AB  - During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank.
AB  - Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia
T1  - Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji
EP  - 129
IS  - 3
SP  - 125
VL  - 55
DO  - 10.5937/RatPov1803125I
ER  - 

@article{
author = "Ignjatov, Maja and Milosević, Dragana and Ivanović, Žarko and Karaman, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica",
year = "2018",
abstract = "During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank., Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia, Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji",
pages = "129-125",
number = "3",
volume = "55",
doi = "10.5937/RatPov1803125I"
}

Ignjatov, M., Milosević, D., Ivanović, Ž., Karaman, M., Vlajić, S., Nikolić, Z.,& Gvozdanović-Varga, J.. (2018). Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 55(3), 125-129.
https://doi.org/10.5937/RatPov1803125I

Ignjatov M, Milosević D, Ivanović Ž, Karaman M, Vlajić S, Nikolić Z, Gvozdanović-Varga J. Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo. 2018;55(3):125-129.
doi:10.5937/RatPov1803125I .

Ignjatov, Maja, Milosević, Dragana, Ivanović, Žarko, Karaman, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia" in Ratarstvo i povrtarstvo, 55, no. 3 (2018):125-129,
https://doi.org/10.5937/RatPov1803125I . .

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Gvozdanović-Varga, J., Marinković, J.,& Ivanović, Ž.. (2017). First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease
American Phytopathological Society., 101(6), 1047-1048.
https://doi.org/10.1094/PDIS-11-16-1625-PDN

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Gvozdanović-Varga J, Marinković J, Ivanović Ž. First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease. 2017;101(6):1047-1048.
doi:10.1094/PDIS-11-16-1625-PDN .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Gvozdanović-Varga, Jelica, Marinković, Jelena, Ivanović, Žarko, "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia" in Plant Disease, 101, no. 6 (2017):1047-1048,
https://doi.org/10.1094/PDIS-11-16-1625-PDN . .

TY  - JOUR
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Marinković, Jelena
AU  - Spremo, Nemanja
AU  - Karaman, Maja
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1044
AB  - Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of indigenous Bacillus spp. isolated from soil
EP  - 269
SP  - 261
VL  - 133
DO  - 10.2298/ZMSPN1733261B
ER  - 

@article{
author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Spremo, Nemanja and Karaman, Maja and Nikolić, Zorica and Ivanović, Žarko",
year = "2017",
abstract = "Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of indigenous Bacillus spp. isolated from soil",
pages = "269-261",
volume = "133",
doi = "10.2298/ZMSPN1733261B"
}

Bjelić, D., Ignjatov, M., Marinković, J., Spremo, N., Karaman, M., Nikolić, Z.,& Ivanović, Ž.. (2017). Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 261-269.
https://doi.org/10.2298/ZMSPN1733261B

Bjelić D, Ignjatov M, Marinković J, Spremo N, Karaman M, Nikolić Z, Ivanović Ž. Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke. 2017;133:261-269.
doi:10.2298/ZMSPN1733261B .

Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Spremo, Nemanja, Karaman, Maja, Nikolić, Zorica, Ivanović, Žarko, "Antifungal activity of indigenous Bacillus spp. isolated from soil" in Zbornik Matice srpske za prirodne nauke, 133 (2017):261-269,
https://doi.org/10.2298/ZMSPN1733261B . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović Milovanović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1026
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
EP  - 17
SP  - 9
VL  - 132
DO  - 10.2298/ZMSPN1732009I
DO  - 0352-4906
ER  - 

@article{
author = "Ignjatov, Maja and Popović Milovanović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)",
pages = "17-9",
volume = "132",
doi = "10.2298/ZMSPN1732009I, 0352-4906"
}

Ignjatov, M., Popović Milovanović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 132, 9-17.
https://doi.org/10.2298/ZMSPN1732009I

Ignjatov M, Popović Milovanović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;132:9-17.
doi:10.2298/ZMSPN1732009I .

Ignjatov, Maja, Popović Milovanović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, D.
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tatić, M.
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/506
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia
EP  - 382
IS  - 2
SP  - 382
VL  - 101
DO  - 10.1094/PDIS-09-16-1333-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Milosević, D. and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tatić, M. and Popović, Tatjana and Ivanović, Žarko",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia",
pages = "382-382",
number = "2",
volume = "101",
doi = "10.1094/PDIS-09-16-1333-PDN"
}

Ignjatov, M., Milosević, D., Nikolić, Z., Gvozdanović-Varga, J., Tatić, M., Popović, T.,& Ivanović, Ž.. (2017). First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(2), 382-382.
https://doi.org/10.1094/PDIS-09-16-1333-PDN

Ignjatov M, Milosević D, Nikolić Z, Gvozdanović-Varga J, Tatić M, Popović T, Ivanović Ž. First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia. in Plant Disease. 2017;101(2):382-382.
doi:10.1094/PDIS-09-16-1333-PDN .

Ignjatov, Maja, Milosević, D., Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tatić, M., Popović, Tatjana, Ivanović, Žarko, "First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia" in Plant Disease, 101, no. 2 (2017):382-382,
https://doi.org/10.1094/PDIS-09-16-1333-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, D.
AU  - Nikolić, Zorica
AU  - Milosević, D.
AU  - Gvozdanović-Varga, Jelica
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/491
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia
EP  - 1048
IS  - 6
SP  - 1047
VL  - 101
DO  - 10.1094/PDIS-11-16-1625-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, D. and Nikolić, Zorica and Milosević, D. and Gvozdanović-Varga, Jelica and Marinković, Jelena and Ivanović, Žarko",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia",
pages = "1048-1047",
number = "6",
volume = "101",
doi = "10.1094/PDIS-11-16-1625-PDN"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milosević, D., Gvozdanović-Varga, J., Marinković, J.,& Ivanović, Ž.. (2017). First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(6), 1047-1048.
https://doi.org/10.1094/PDIS-11-16-1625-PDN

Ignjatov M, Bjelić D, Nikolić Z, Milosević D, Gvozdanović-Varga J, Marinković J, Ivanović Ž. First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease. 2017;101(6):1047-1048.
doi:10.1094/PDIS-11-16-1625-PDN .

Ignjatov, Maja, Bjelić, D., Nikolić, Zorica, Milosević, D., Gvozdanović-Varga, Jelica, Marinković, Jelena, Ivanović, Žarko, "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia" in Plant Disease, 101, no. 6 (2017):1047-1048,
https://doi.org/10.1094/PDIS-11-16-1625-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/460
AB  - During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
AB  - Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
T1  - Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji
EP  - 45
IS  - 2
SP  - 42
VL  - 53
DO  - 10.5937/ratpov53-9984
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Vasić, Mirjana and Nikolić, Zorica and Tamindžić, Gordana and Ivanović, Žarko",
year = "2016",
abstract = "During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot., Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia, Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji",
pages = "45-42",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9984"
}

Ignjatov, M., Popović, T., Milošević, D., Vasić, M., Nikolić, Z., Tamindžić, G.,& Ivanović, Ž.. (2016). Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 53(2), 42-45.
https://doi.org/10.5937/ratpov53-9984

Ignjatov M, Popović T, Milošević D, Vasić M, Nikolić Z, Tamindžić G, Ivanović Ž. Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo. 2016;53(2):42-45.
doi:10.5937/ratpov53-9984 .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Vasić, Mirjana, Nikolić, Zorica, Tamindžić, Gordana, Ivanović, Žarko, "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia" in Ratarstvo i povrtarstvo, 53, no. 2 (2016):42-45,
https://doi.org/10.5937/ratpov53-9984 . .

Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J., Ivanović, Ž.,& Popović Milovanović, T.. (2015). First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia. in Plant Disease
American Phytopathological Society., 99(9), 1277-1278.
https://doi.org/10.1094/PDIS-01-15-0082-PDN

Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž, Popović Milovanović T. First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia. in Plant Disease. 2015;99(9):1277-1278.
doi:10.1094/PDIS-01-15-0082-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, Popović Milovanović, Tatjana, "First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia" in Plant Disease, 99, no. 9 (2015):1277-1278,
https://doi.org/10.1094/PDIS-01-15-0082-PDN . .

Ignjatov, M., Gvozdanović-Varga, J., Milošević, d., Nikolić, Z., Ivanović, Ž.,& Popović Milovanović, T.. (2015). First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia. in Plant Disease
American Phytopathological Society., 99(5), 723.
https://doi.org/10.1094/PDIS-10-14-1097-PDN

Ignjatov M, Gvozdanović-Varga J, Milošević D, Nikolić Z, Ivanović Ž, Popović Milovanović T. First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia. in Plant Disease. 2015;99(5):723.
doi:10.1094/PDIS-10-14-1097-PDN .

Ignjatov, Maja, Gvozdanović-Varga, Jelica, Milošević, dr, Nikolić, Zorica, Ivanović, Žarko, Popović Milovanović, Tatjana, "First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia" in Plant Disease, 99, no. 5 (2015):723,
https://doi.org/10.1094/PDIS-10-14-1097-PDN . .

Milošević, D., Gvozdanović-Varga, J., Ignjatov, M., Nikolić, Z., Vučurović, I., Vučurović, A.,& Stanković, I.. (2015). First Report of Onion yellow dwarf virus Infecting Shallot in Serbia. in Plant Disease
American Phytopathological Society., 99(10), 1450.
https://doi.org/10.1094/PDIS-04-15-0370-PDN

Milošević D, Gvozdanović-Varga J, Ignjatov M, Nikolić Z, Vučurović I, Vučurović A, Stanković I. First Report of Onion yellow dwarf virus Infecting Shallot in Serbia. in Plant Disease. 2015;99(10):1450.
doi:10.1094/PDIS-04-15-0370-PDN .

Milošević, Dragana, Gvozdanović-Varga, Jelica, Ignjatov, Maja, Nikolić, Zorica, Vučurović, Ivan, Vučurović, Ana, Stanković, Ivana, "First Report of Onion yellow dwarf virus Infecting Shallot in Serbia" in Plant Disease, 99, no. 10 (2015):1450,
https://doi.org/10.1094/PDIS-04-15-0370-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, D.
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/399
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia.
EP  - 1278
IS  - 9
SP  - 1277
VL  - 99
DO  - 10.1094/PDIS-01-15-0082-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Milosević, D. and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko and Popović, Tatjana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia.",
pages = "1278-1277",
number = "9",
volume = "99",
doi = "10.1094/PDIS-01-15-0082-PDN"
}

Ignjatov, M., Milosević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J., Ivanović, Ž.,& Popović, T.. (2015). First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(9), 1277-1278.
https://doi.org/10.1094/PDIS-01-15-0082-PDN

Ignjatov M, Milosević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž, Popović T. First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia.. in Plant Disease. 2015;99(9):1277-1278.
doi:10.1094/PDIS-01-15-0082-PDN .

Ignjatov, Maja, Milosević, D., Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, Popović, Tatjana, "First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia." in Plant Disease, 99, no. 9 (2015):1277-1278,
https://doi.org/10.1094/PDIS-01-15-0082-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gvozdanović-Varga, Jelica
AU  - Milosević, D.
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/414
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia
EP  - 723
IS  - 5
SP  - 723
VL  - 99
DO  - 10.1094/PDIS-10-14-1097-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Gvozdanović-Varga, Jelica and Milosević, D. and Nikolić, Zorica and Ivanović, Žarko and Popović, Tatjana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia",
pages = "723-723",
number = "5",
volume = "99",
doi = "10.1094/PDIS-10-14-1097-PDN"
}

Ignjatov, M., Gvozdanović-Varga, J., Milosević, D., Nikolić, Z., Ivanović, Ž.,& Popović, T.. (2015). First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 723-723.
https://doi.org/10.1094/PDIS-10-14-1097-PDN

Ignjatov M, Gvozdanović-Varga J, Milosević D, Nikolić Z, Ivanović Ž, Popović T. First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia. in Plant Disease. 2015;99(5):723-723.
doi:10.1094/PDIS-10-14-1097-PDN .

Ignjatov, Maja, Gvozdanović-Varga, Jelica, Milosević, D., Nikolić, Zorica, Ivanović, Žarko, Popović, Tatjana, "First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia" in Plant Disease, 99, no. 5 (2015):723-723,
https://doi.org/10.1094/PDIS-10-14-1097-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1135
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 

@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}

Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769

Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .

Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Balaž, Jelica
AU  - Nikolić, Zorica
AU  - Starović, Mira
AU  - Gavrilović, Veljko
AU  - Aleksić, Goran
AU  - Vasić, Mirjana
AU  - Živković, Svetlana
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/123
AB  - Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.
PB  - Academic Journals, Victoria Island
T2  - African Journal of Agricultural Research
T1  - Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia
EP  - 2736
IS  - 19
SP  - 2730
VL  - 5
ER  - 

@article{
author = "Popović, Tatjana and Balaž, Jelica and Nikolić, Zorica and Starović, Mira and Gavrilović, Veljko and Aleksić, Goran and Vasić, Mirjana and Živković, Svetlana",
year = "2010",
abstract = "Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.",
publisher = "Academic Journals, Victoria Island",
journal = "African Journal of Agricultural Research",
title = "Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia",
pages = "2736-2730",
number = "19",
volume = "5"
}

Popović, T., Balaž, J., Nikolić, Z., Starović, M., Gavrilović, V., Aleksić, G., Vasić, M.,& Živković, S.. (2010). Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia. in African Journal of Agricultural Research
Academic Journals, Victoria Island., 5(19), 2730-2736.

Popović T, Balaž J, Nikolić Z, Starović M, Gavrilović V, Aleksić G, Vasić M, Živković S. Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia. in African Journal of Agricultural Research. 2010;5(19):2730-2736..

Popović, Tatjana, Balaž, Jelica, Nikolić, Zorica, Starović, Mira, Gavrilović, Veljko, Aleksić, Goran, Vasić, Mirjana, Živković, Svetlana, "Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia" in African Journal of Agricultural Research, 5, no. 19 (2010):2730-2736.

TY  - JOUR
AU  - Balaž, Jelica
AU  - Popović Milovanović, Tatjana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1066
AB  - U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja
EP  - 88
IS  - 2
SP  - 81
VL  - 23
DO  - 10.2298/PIF0802081B
ER  - 

@article{
author = "Balaž, Jelica and Popović Milovanović, Tatjana and Vasić, Mirjana and Nikolić, Zorica",
year = "2008",
abstract = "U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja",
pages = "88-81",
number = "2",
volume = "23",
doi = "10.2298/PIF0802081B"
}

Balaž, J., Popović Milovanović, T., Vasić, M.,& Nikolić, Z.. (2008). Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 23(2), 81-88.
https://doi.org/10.2298/PIF0802081B

Balaž J, Popović Milovanović T, Vasić M, Nikolić Z. Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja. in Pesticidi i fitomedicina. 2008;23(2):81-88.
doi:10.2298/PIF0802081B .

Balaž, Jelica, Popović Milovanović, Tatjana, Vasić, Mirjana, Nikolić, Zorica, "Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja" in Pesticidi i fitomedicina, 23, no. 2 (2008):81-88,
https://doi.org/10.2298/PIF0802081B . .

TY  - JOUR
AU  - Balaž, Jelica
AU  - Popović Milovanović, Tatjana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1063
AB  - method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds
EP  - 98
IS  - 2
SP  - 89
VL  - 23
DO  - 10.2298/PIF0802089B
ER  - 

@article{
author = "Balaž, Jelica and Popović Milovanović, Tatjana and Vasić, Mirjana and Nikolić, Zorica",
year = "2008",
abstract = "method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds",
pages = "98-89",
number = "2",
volume = "23",
doi = "10.2298/PIF0802089B"
}

Balaž, J., Popović Milovanović, T., Vasić, M.,& Nikolić, Z.. (2008). Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 23(2), 89-98.
https://doi.org/10.2298/PIF0802089B

Balaž J, Popović Milovanović T, Vasić M, Nikolić Z. Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds. in Pesticidi i fitomedicina. 2008;23(2):89-98.
doi:10.2298/PIF0802089B .

Balaž, Jelica, Popović Milovanović, Tatjana, Vasić, Mirjana, Nikolić, Zorica, "Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds" in Pesticidi i fitomedicina, 23, no. 2 (2008):89-98,
https://doi.org/10.2298/PIF0802089B . .