TY  - CHAP
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Oro, Violeta
PY  - 2022
UR  - https://enauka.gov.rs/handle/123456789/849595
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1191
AB  - Sugar beet (Beta vulgaris L.) has been grown for centuries as one of the most important agricultural plants worldwide for the purpose of white crystal sugar production. A large number of pathogens are present on the sugar beet, causing a large number of various symptoms and damaging production in a big scale. Plant diseases and pests are present on leaves and roots, causing damage to the above- and below-ground parts. Rhizoctonia solani Kuhn. is one such disease that attacks the sugar beet and is widespread throughout the world. R. solani is a soil-borne pathogen that survives in soil, where its life cycle is maintained for a long time period. It grows in the temperate and continental climate zone, making it a very important pathogen with respect to this crop due to its favourable condition. It overwinters in the soil for many years in the form of sclerotia or mycelium. The first symptoms appear on the upper part of sugar beet plants; usually symptomatic plants appear in bordered zones, often present in certain parts of the field with a clearly bordered belt. The infected plants have lost their turgor, and most of the leaf rosette gets laid on the ground. In the cross section, the central part of the root is initially healthy, while the disease is primarily observed on the rim of the root. The most important and only fully effective approach in controlling R. solani is the application of fungicides from SDHI and QoI groups at the appropriate time of application.
PB  - Springer
T2  - In: Sugar Beet Cultivation, Management and Processing (eds. Misra, V., Srivastava, S., Mall, A.K.), Springer, Singapore
T1  - Rhizoctonia Disease and Its Management
EP  - 811
SP  - 793
DO  - 10.1007/978-981-19-2730-0_39
ER  - 

@inbook{
author = "Trkulja, Nenad and Milosavljević, Anja and Oro, Violeta",
year = "2022",
abstract = "Sugar beet (Beta vulgaris L.) has been grown for centuries as one of the most important agricultural plants worldwide for the purpose of white crystal sugar production. A large number of pathogens are present on the sugar beet, causing a large number of various symptoms and damaging production in a big scale. Plant diseases and pests are present on leaves and roots, causing damage to the above- and below-ground parts. Rhizoctonia solani Kuhn. is one such disease that attacks the sugar beet and is widespread throughout the world. R. solani is a soil-borne pathogen that survives in soil, where its life cycle is maintained for a long time period. It grows in the temperate and continental climate zone, making it a very important pathogen with respect to this crop due to its favourable condition. It overwinters in the soil for many years in the form of sclerotia or mycelium. The first symptoms appear on the upper part of sugar beet plants; usually symptomatic plants appear in bordered zones, often present in certain parts of the field with a clearly bordered belt. The infected plants have lost their turgor, and most of the leaf rosette gets laid on the ground. In the cross section, the central part of the root is initially healthy, while the disease is primarily observed on the rim of the root. The most important and only fully effective approach in controlling R. solani is the application of fungicides from SDHI and QoI groups at the appropriate time of application.",
publisher = "Springer",
journal = "In: Sugar Beet Cultivation, Management and Processing (eds. Misra, V., Srivastava, S., Mall, A.K.), Springer, Singapore",
booktitle = "Rhizoctonia Disease and Its Management",
pages = "811-793",
doi = "10.1007/978-981-19-2730-0_39"
}

Trkulja, N., Milosavljević, A.,& Oro, V.. (2022). Rhizoctonia Disease and Its Management. in In: Sugar Beet Cultivation, Management and Processing (eds. Misra, V., Srivastava, S., Mall, A.K.), Springer, Singapore
Springer., 793-811.
https://doi.org/10.1007/978-981-19-2730-0_39

Trkulja N, Milosavljević A, Oro V. Rhizoctonia Disease and Its Management. in In: Sugar Beet Cultivation, Management and Processing (eds. Misra, V., Srivastava, S., Mall, A.K.), Springer, Singapore. 2022;:793-811.
doi:10.1007/978-981-19-2730-0_39 .

Trkulja, Nenad, Milosavljević, Anja, Oro, Violeta, "Rhizoctonia Disease and Its Management" in In: Sugar Beet Cultivation, Management and Processing (eds. Misra, V., Srivastava, S., Mall, A.K.), Springer, Singapore (2022):793-811,
https://doi.org/10.1007/978-981-19-2730-0_39 . .

TY  - CHAP
AU  - Oro, Violeta
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Sečanski, Mile
AU  - Tabaković, Marijenka
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/970
AB  - Sugar beet is listed within the top ten most important crops in the world. The paleobotanic data suggest the sea beet was grown in the ancient times, while the beets with swollen roots were cultivated in the Middle Ages in Europe. Sugar beet cyst nematode Heterodera schachtii is an invasive organism causing high economic loss to sugar beet worldwide. The fundamental steps in control of harmful organisms in plant protection and food safety are grounded on rapid detection of the causative agent and its proper identification. The prompt reaction before obvious symptoms occur can prevent devastating consequences. To confirm the identity of an invasive organism, the process demands the combination of identification techniques, such as morphology and molecular characterization. The phylogeography of available H. schachtii populations, based on matching historical data with phylogenetic analyses of the ITS rRNA region pinpointed a possible place of origin of the European H. schachtii populations. A next step is the determination of natural antagonists of nematodes. Due to the long persistence of the parasite in soil, cysts harbor a large number of bacteria and fungi, the presence of which can lead to cyst death and population decline. Bacteria, fungi and other antagonists are an inevitable part of the soil ecosystem and are also part of those mechanisms in nature that limit the excessive number of invasive organisms and return ecological system to its stable equilibrium, acceptable for the existence of humans as well as micro and macrobiota on Earth.
PB  - Springer Nature Singapore Pte Ltd.
T2  - Springer Nature
T1  - Sugar Beet Cyst Nematode (Heterodera schachtii Schmidt): Identification and Antagonists
EP  - 776
SP  - 751
VL  - 1
ER  - 

@inbook{
author = "Oro, Violeta and Trkulja, Nenad and Milosavljević, Anja and Sečanski, Mile and Tabaković, Marijenka",
year = "2021",
abstract = "Sugar beet is listed within the top ten most important crops in the world. The paleobotanic data suggest the sea beet was grown in the ancient times, while the beets with swollen roots were cultivated in the Middle Ages in Europe. Sugar beet cyst nematode Heterodera schachtii is an invasive organism causing high economic loss to sugar beet worldwide. The fundamental steps in control of harmful organisms in plant protection and food safety are grounded on rapid detection of the causative agent and its proper identification. The prompt reaction before obvious symptoms occur can prevent devastating consequences. To confirm the identity of an invasive organism, the process demands the combination of identification techniques, such as morphology and molecular characterization. The phylogeography of available H. schachtii populations, based on matching historical data with phylogenetic analyses of the ITS rRNA region pinpointed a possible place of origin of the European H. schachtii populations. A next step is the determination of natural antagonists of nematodes. Due to the long persistence of the parasite in soil, cysts harbor a large number of bacteria and fungi, the presence of which can lead to cyst death and population decline. Bacteria, fungi and other antagonists are an inevitable part of the soil ecosystem and are also part of those mechanisms in nature that limit the excessive number of invasive organisms and return ecological system to its stable equilibrium, acceptable for the existence of humans as well as micro and macrobiota on Earth.",
publisher = "Springer Nature Singapore Pte Ltd.",
journal = "Springer Nature",
booktitle = "Sugar Beet Cyst Nematode (Heterodera schachtii Schmidt): Identification and Antagonists",
pages = "776-751",
volume = "1"
}

Oro, V., Trkulja, N., Milosavljević, A., Sečanski, M.,& Tabaković, M.. (2021). Sugar Beet Cyst Nematode (Heterodera schachtii Schmidt): Identification and Antagonists. in Springer Nature
Springer Nature Singapore Pte Ltd.., 1, 751-776.

Oro V, Trkulja N, Milosavljević A, Sečanski M, Tabaković M. Sugar Beet Cyst Nematode (Heterodera schachtii Schmidt): Identification and Antagonists. in Springer Nature. 2021;1:751-776..

Oro, Violeta, Trkulja, Nenad, Milosavljević, Anja, Sečanski, Mile, Tabaković, Marijenka, "Sugar Beet Cyst Nematode (Heterodera schachtii Schmidt): Identification and Antagonists" in Springer Nature, 1 (2021):751-776.

TY  - CONF
AU  - Dervišević, Marina
AU  - Đorđević, Nikola
AU  - Knežević, Isidora
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Đorđević, Snežana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/820
AB  - Sclerotinia sclerotiorum (Lib.), prouzrokovač bele truleži, široko je rasprostranjena i prouzrokuje štete na više od 400 biljaka domaćina, uključujući brojne ratarske i povrtarske biljke, kako u polju, tako i u skladišnim uslovima. Kako plodored i hemijske metode, često nisu dovoljno efikasni u kontroli ovog patogena, cilj ovog rada bio je da se ispita efikasnost bakterijskih izolata roda Bacillus u suzbijanju S. sclerotiorum. Antagonistička aktivnost 6 autohtonih bakterijskih izolata (Bacillus subtilis Z3, Bacillus mojavensis 5B2, Bacillus mojavensis R6, Bacillus firmus S5, Bacillus proteolyticus 7E i Bacillus thuringiensis 109 /18 1.) ispitivana je metodom dvojne kultivacije. U Petri kutijama sa krompir-dekstroznim agarom (PDA) zasejani su fragmenti (Ø8 mm) izolata S. sclerotiorum, a potom su bakteriološkom ezom, na udaljenosti 3 cm od fragmenata gljiva, naneti bakterijski sojevi. U kontrolnom tretmanu, naneti su fragmenti micelije izolata fitopatogene gljjive bez prisustva bakterijskog soja. Ogled je izveden u tri ponavljanja po tretmanu. Očitavanje 35 rezultata je urađeno nakon inkubacije u trajanju od 7 dana na temperaturi od 25 ± 1oC, merenjem prečnika inhibicije. Bakterijski sojevi koji su pokazali najveću efikasnost u ovom testu, ispitivani su i u biotestovima na listovima kupusa i krtolastom povrću (mrkva i paštrnak) u laboratorijskim uslovima. Bakterijski izolati Bacillus mojavensis 5B2, Bacillus mojavensis R-6 i Bacillus thuringiensis 109/18 1. pokazali su antifungalnu aktivnost prema Sclerotinia sclerotiorum. Najveći procenat inhibicije (PIRG) zabeležen je kod Bacillus mojavensis R6 (56.47%), zatim kod B. mojavensis 5B2 (49.41%) i B. thuringiensis 109/18 1. (48.24%), dok Bacillus subtilis Z3, Bacillus firmus S5 i Bacillus proteolyticus 7E nisu pokazali antifungalnu aktivnost prema S. sclerociorum. U biotestovima, bakterijski sojevi Bacillus mojavensis R6, B. mojavensis 5B2 i B. thuringiensis 109/18 1. inhibirali su porast micelije i sklerocija i na taj način sprečili razvoj S. sclerotiorum, za razliku od kontrolnih tretmana u kojima je došlo do formiranja karakterističnih simptoma koje ovaj patogen prouzrokuje. Iz tog razloga, ovi bakterijski sojevi mogu biti potencijalni biološki agensi za kontrolu S. sclerotiorum, na ekološki prihvatljiv i siguran način.
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35
T1  - Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum
ER  - 

@conference{
author = "Dervišević, Marina and Đorđević, Nikola and Knežević, Isidora and Trkulja, Nenad and Milosavljević, Anja and Đorđević, Snežana",
year = "2021",
abstract = "Sclerotinia sclerotiorum (Lib.), prouzrokovač bele truleži, široko je rasprostranjena i prouzrokuje štete na više od 400 biljaka domaćina, uključujući brojne ratarske i povrtarske biljke, kako u polju, tako i u skladišnim uslovima. Kako plodored i hemijske metode, često nisu dovoljno efikasni u kontroli ovog patogena, cilj ovog rada bio je da se ispita efikasnost bakterijskih izolata roda Bacillus u suzbijanju S. sclerotiorum. Antagonistička aktivnost 6 autohtonih bakterijskih izolata (Bacillus subtilis Z3, Bacillus mojavensis 5B2, Bacillus mojavensis R6, Bacillus firmus S5, Bacillus proteolyticus 7E i Bacillus thuringiensis 109 /18 1.) ispitivana je metodom dvojne kultivacije. U Petri kutijama sa krompir-dekstroznim agarom (PDA) zasejani su fragmenti (Ø8 mm) izolata S. sclerotiorum, a potom su bakteriološkom ezom, na udaljenosti 3 cm od fragmenata gljiva, naneti bakterijski sojevi. U kontrolnom tretmanu, naneti su fragmenti micelije izolata fitopatogene gljjive bez prisustva bakterijskog soja. Ogled je izveden u tri ponavljanja po tretmanu. Očitavanje 35 rezultata je urađeno nakon inkubacije u trajanju od 7 dana na temperaturi od 25 ± 1oC, merenjem prečnika inhibicije. Bakterijski sojevi koji su pokazali najveću efikasnost u ovom testu, ispitivani su i u biotestovima na listovima kupusa i krtolastom povrću (mrkva i paštrnak) u laboratorijskim uslovima. Bakterijski izolati Bacillus mojavensis 5B2, Bacillus mojavensis R-6 i Bacillus thuringiensis 109/18 1. pokazali su antifungalnu aktivnost prema Sclerotinia sclerotiorum. Najveći procenat inhibicije (PIRG) zabeležen je kod Bacillus mojavensis R6 (56.47%), zatim kod B. mojavensis 5B2 (49.41%) i B. thuringiensis 109/18 1. (48.24%), dok Bacillus subtilis Z3, Bacillus firmus S5 i Bacillus proteolyticus 7E nisu pokazali antifungalnu aktivnost prema S. sclerociorum. U biotestovima, bakterijski sojevi Bacillus mojavensis R6, B. mojavensis 5B2 i B. thuringiensis 109/18 1. inhibirali su porast micelije i sklerocija i na taj način sprečili razvoj S. sclerotiorum, za razliku od kontrolnih tretmana u kojima je došlo do formiranja karakterističnih simptoma koje ovaj patogen prouzrokuje. Iz tog razloga, ovi bakterijski sojevi mogu biti potencijalni biološki agensi za kontrolu S. sclerotiorum, na ekološki prihvatljiv i siguran način.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35",
title = "Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum"
}

Dervišević, M., Đorđević, N., Knežević, I., Trkulja, N., Milosavljević, A.,& Đorđević, S.. (2021). Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35
Društvo za zaštitu bilja..

Dervišević M, Đorđević N, Knežević I, Trkulja N, Milosavljević A, Đorđević S. Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35. 2021;..

Dervišević, Marina, Đorđević, Nikola, Knežević, Isidora, Trkulja, Nenad, Milosavljević, Anja, Đorđević, Snežana, "Efikasnost autohtonih bakterijskih izolata u suzbijanju Sclerotinia sclerotiorum" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35 (2021).

TY  - CONF
AU  - Đorđević, Nikola
AU  - Dervišević, Marina
AU  - Knežević, Isidora
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Đorđević, Snežana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/806
AB  - Botrytis cinerea, prouzrokovač sive truleži, široko je rasprostranjen i štetan patogen na velikom broju biljaka, uključujući vinovu lozu i voće, kao i povrće, naročito u stakleničkoj i plasteničkoj proizvodnji. Za suzbijanje ovog patogena primenjuju se različiti fungicidi hemijske prirode, a u poslednje vreme, primenu nalaze i brojni biokontrolni agensi, uključujući bakterije roda Bacillus, zbog čega je cilj ovog rada bio da se ispita antifungalna aktivnost bakterijskih sojeva u suzbijanju Botrytis cinerea. U ovom istraživanju, korišćeno je 12 bakterijskih sojeva roda Bacillus (Bacillus velezensis 31/18 RK 1-1, Bacillus amyloliquefaciens 31/18 RK 1-2, Bacillus licheniformis 31/18 RS-9, Bacillus mojavensis 31/18 5b2, Bacillus aerius 92/17 7, Bacillus marisflavi 6E, Bacillus pumilus W27-2, Bacillus macroides W29-5, Bacillus megaterium S1, Bacillus thuringiensis BCE, Bacillus amyloliquefaciens B002, Bacillus proteolyticus 7E). Antifungalna aktivnost ispitivanih sojeva roda Bacillus prema Botrytis cinerea, testirana je u laboratorijskim uslovima, metodom 68 dvojne kultivacije, a nakon 7 dana inkubacije očitavani su rezultati. Svi ispitivani sojevi bakterija, testirani su i na plodovima krastavca, u različitim koncentracijama primene (1%, 0,5% i 0,1%). Rezultati ispitivanja antifungalne aktivnosti bakterijskih sojeva roda Bacillus prema B. cinerea pokazali su da je najveći procenat inhibicije od 57,7% zabeležen kod Bacillus amyloliquefaciens 31/18 RK 1-2, zatim 50,7% kod Bacillus velezensis 31/18 RK 1-1 i 45,4% kod Bacillus mojavensis 31/18 5b2, dok su ostali sojevi pokazali manju inhibitornu aktivnost. U bitestu na plodovima krastavca najbolji rezultati su zabeleženi u tretmanu sa Bacillus licheniformis 31/18 RS-9 gde je rast micelije B. cinerea zaustavljen u tretmanima sa svakom od primenjenih koncentracija. Takođe, dobro antagonističko delovanje pokazalo se i kod Bacillus mojavensis 31/18 5b2 pri primenjenim koncentracijama 1 i 0.5%. Iz tog razloga, bakterije roda Bacillus imaju dobar potencijal za upotrebu u suzbijanju fitopatogene gljive B. cinerea.
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, Zlatibor 22-25. novembar 2021: 67-68
T1  - Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea
ER  - 

@conference{
author = "Đorđević, Nikola and Dervišević, Marina and Knežević, Isidora and Trkulja, Nenad and Milosavljević, Anja and Đorđević, Snežana",
year = "2021",
abstract = "Botrytis cinerea, prouzrokovač sive truleži, široko je rasprostranjen i štetan patogen na velikom broju biljaka, uključujući vinovu lozu i voće, kao i povrće, naročito u stakleničkoj i plasteničkoj proizvodnji. Za suzbijanje ovog patogena primenjuju se različiti fungicidi hemijske prirode, a u poslednje vreme, primenu nalaze i brojni biokontrolni agensi, uključujući bakterije roda Bacillus, zbog čega je cilj ovog rada bio da se ispita antifungalna aktivnost bakterijskih sojeva u suzbijanju Botrytis cinerea. U ovom istraživanju, korišćeno je 12 bakterijskih sojeva roda Bacillus (Bacillus velezensis 31/18 RK 1-1, Bacillus amyloliquefaciens 31/18 RK 1-2, Bacillus licheniformis 31/18 RS-9, Bacillus mojavensis 31/18 5b2, Bacillus aerius 92/17 7, Bacillus marisflavi 6E, Bacillus pumilus W27-2, Bacillus macroides W29-5, Bacillus megaterium S1, Bacillus thuringiensis BCE, Bacillus amyloliquefaciens B002, Bacillus proteolyticus 7E). Antifungalna aktivnost ispitivanih sojeva roda Bacillus prema Botrytis cinerea, testirana je u laboratorijskim uslovima, metodom 68 dvojne kultivacije, a nakon 7 dana inkubacije očitavani su rezultati. Svi ispitivani sojevi bakterija, testirani su i na plodovima krastavca, u različitim koncentracijama primene (1%, 0,5% i 0,1%). Rezultati ispitivanja antifungalne aktivnosti bakterijskih sojeva roda Bacillus prema B. cinerea pokazali su da je najveći procenat inhibicije od 57,7% zabeležen kod Bacillus amyloliquefaciens 31/18 RK 1-2, zatim 50,7% kod Bacillus velezensis 31/18 RK 1-1 i 45,4% kod Bacillus mojavensis 31/18 5b2, dok su ostali sojevi pokazali manju inhibitornu aktivnost. U bitestu na plodovima krastavca najbolji rezultati su zabeleženi u tretmanu sa Bacillus licheniformis 31/18 RS-9 gde je rast micelije B. cinerea zaustavljen u tretmanima sa svakom od primenjenih koncentracija. Takođe, dobro antagonističko delovanje pokazalo se i kod Bacillus mojavensis 31/18 5b2 pri primenjenim koncentracijama 1 i 0.5%. Iz tog razloga, bakterije roda Bacillus imaju dobar potencijal za upotrebu u suzbijanju fitopatogene gljive B. cinerea.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, Zlatibor 22-25. novembar 2021: 67-68",
title = "Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea"
}

Đorđević, N., Dervišević, M., Knežević, I., Trkulja, N., Milosavljević, A.,& Đorđević, S.. (2021). Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, Zlatibor 22-25. novembar 2021: 67-68
Društvo za zaštitu bilja Srbije..

Đorđević N, Dervišević M, Knežević I, Trkulja N, Milosavljević A, Đorđević S. Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, Zlatibor 22-25. novembar 2021: 67-68. 2021;..

Đorđević, Nikola, Dervišević, Marina, Knežević, Isidora, Trkulja, Nenad, Milosavljević, Anja, Đorđević, Snežana, "Antagonistička aktivnost bakterijskih izolata roda Bacillus prema Botrytis cinerea" in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, Zlatibor 22-25. novembar 2021: 67-68 (2021).

TY  - CONF
AU  - Chittem, K.
AU  - Milosavljević, Anja
AU  - Mendoza, L. E. del Rio
AU  - Khan, M.
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/520
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Detection of QoI fungicide resistant Cercospora beticola airborne inoculum using quantitative PCR
EP  - 23
IS  - 10
SP  - 22
VL  - 108
ER  - 

@conference{
author = "Chittem, K. and Milosavljević, Anja and Mendoza, L. E. del Rio and Khan, M.",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Detection of QoI fungicide resistant Cercospora beticola airborne inoculum using quantitative PCR",
pages = "23-22",
number = "10",
volume = "108"
}

Chittem, K., Milosavljević, A., Mendoza, L. E. d. R.,& Khan, M.. (2018). Detection of QoI fungicide resistant Cercospora beticola airborne inoculum using quantitative PCR. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 22-23.

Chittem K, Milosavljević A, Mendoza LEDR, Khan M. Detection of QoI fungicide resistant Cercospora beticola airborne inoculum using quantitative PCR. in Phytopathology. 2018;108(10):22-23..

Chittem, K., Milosavljević, Anja, Mendoza, L. E. del Rio, Khan, M., "Detection of QoI fungicide resistant Cercospora beticola airborne inoculum using quantitative PCR" in Phytopathology, 108, no. 10 (2018):22-23.

Trkulja, N.,& Milosavljević, A.. (2017). Inovativna tehnologija primene fungicida za kontrolu prouzrokovača pegavosti lišća šećerne repe Cercospora beticola Sacc.. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Trkulja N, Milosavljević A. Inovativna tehnologija primene fungicida za kontrolu prouzrokovača pegavosti lišća šećerne repe Cercospora beticola Sacc.. in Institut za zaštitu bilja i životnu sredinu. 2017;..

Trkulja, Nenad, Milosavljević, Anja, "Inovativna tehnologija primene fungicida za kontrolu prouzrokovača pegavosti lišća šećerne repe Cercospora beticola Sacc." in Institut za zaštitu bilja i životnu sredinu (2017).

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Khan, Mohamed F. R.
AU  - Secor, Gary A.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/475
AB  - Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides
EP  - 910
IS  - 4
SP  - 895
VL  - 149
DO  - 10.1007/s10658-017-1239-0
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Khan, Mohamed F. R. and Secor, Gary A.",
year = "2017",
abstract = "Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides",
pages = "910-895",
number = "4",
volume = "149",
doi = "10.1007/s10658-017-1239-0"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J., Toševski, I., Khan, M. F. R.,& Secor, G. A.. (2017). Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology
Springer, Dordrecht., 149(4), 895-910.
https://doi.org/10.1007/s10658-017-1239-0

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I, Khan MFR, Secor GA. Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology. 2017;149(4):895-910.
doi:10.1007/s10658-017-1239-0 .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Khan, Mohamed F. R., Secor, Gary A., "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides" in European Journal of Plant Pathology, 149, no. 4 (2017):895-910,
https://doi.org/10.1007/s10658-017-1239-0 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Milosavljević, Anja
AU  - Bosković, J.
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/455
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia
EP  - 221
IS  - 1
SP  - 221
VL  - 100
DO  - 10.1094/PDIS-02-15-0209-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Pfaf-Dolovac, Erika and Milosavljević, Anja and Bosković, J. and Jović, Jelena and Mitrović, Milana and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia",
pages = "221-221",
number = "1",
volume = "100",
doi = "10.1094/PDIS-02-15-0209-PDN"
}

Trkulja, N., Pfaf-Dolovac, E., Milosavljević, A., Bosković, J., Jović, J., Mitrović, M.,& Toševski, I.. (2016). First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 221-221.
https://doi.org/10.1094/PDIS-02-15-0209-PDN

Trkulja N, Pfaf-Dolovac E, Milosavljević A, Bosković J, Jović J, Mitrović M, Toševski I. First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease. 2016;100(1):221-221.
doi:10.1094/PDIS-02-15-0209-PDN .

Trkulja, Nenad, Pfaf-Dolovac, Erika, Milosavljević, Anja, Bosković, J., Jović, Jelena, Mitrović, Milana, Toševski, Ivo, "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia" in Plant Disease, 100, no. 1 (2016):221-221,
https://doi.org/10.1094/PDIS-02-15-0209-PDN . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Ivanović, Žarko
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Ignjatov, Maja
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/945
AB  - In May 2014, during a cool, humid spring period, bacterial spot symptoms were observed for the first time on pea (Pisum sativum L.) in a home garden in the Bačka region of Vojvodina Province in Serbia. Commercial pea is grown on 11,533 ha, as well as in small vegetable farms in Serbia, with average yields of 4 t/ha. Observed spots on leaves were necrotic and progressed inward from the margins. Disease incidence approached 10 to 30%. Bacteria consistently isolated from margins of leaf lesions macerated in sterile phosphate buffered saline (PBS, pH 7.4) formed round, shiny, white, 2- to 3-mm in diameter colonies on nutrient agar with 5% (w/v) sucrose (NAS) and a green fluorescent pigment on King’s medium B (KB). A collection of 20 strains selected for further study was strictly aerobic, gram-negative; positive for levan and tobacco hypersensitive response and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Strains were characterized by repetitive (rep)-PCR, sequence typing, and pathogenicity. To determine genetic heterogenicity among the tested strains, rep-PCR genetic fingerprinting was conducted using the REP, ERIC, and BOX primers (Louws et al. 1994). Profile analyses yielded identical banding patterns for all tested strains. To identify three representative strains by sequence typing, the housekeeping genes gyrB and gltA were amplified (Ferrante and Scortichini 2010). Sequences were deposited in GenBank under accession numbers KP188575 to 77 for gyrB and KP188578 to 80 for gltA. BLAST analysis showed that the regions were 100% homologous for the partial gltA gene of the reference strain P. syringae pv. syringae CFBP 4702 (KF937504) and 99% homologous for the partial gyrB gene of the reference strain P. syringae pv. syringae PD 2021 (KJ158894). Pathogenicity of the three tested strains was tested by using lesion tests and stem inoculation (Mazarei and Kerr 1990). For all tests, a reference strain P. syringae pv. syringae GSPB 1142 and PBS served as a positive and negative controls, respectively. In lesion tests, young bean pods and pale yellowish-green lemon fruits were inoculated by placing drops of bacterial suspension (with 108 CFU/ml) on the surface and pricking lightly through the drops with a sterile needle. Fruits and pods were incubated at 25°C for 7 days. Tested strains produced small reddish-brown depressed lesions on bean pods and dark brown depressed lesions on lemon fruits. For stem inoculation, pea cv. Mali provansalac plantlets were grown from seed for 10 to 14 days. Bacteria grown on KB for 24 h were scraped from the surface with a sterile needle and stabbed into the main stem at the youngest two nodes. Inoculation resulted in extensive necrosis and collapse with stunting on the plants 10 days after inoculation. Control strain GSPB 1142 developed similar symptoms to those of the strains tested. No symptoms were observed on plants inoculated with PBS. The bacterium was reisolated from lesions of test plants for the tree tested strains and confirmed to be the same bacterium using LOPAT tests and rep-PCR genetic fingerprinting. To our knowledge, this is the first report of P. syringae pv. syringae causing bacterial brown spot of pea in Serbia. The information of this species as a pea pathogen is still limited but is considered to be one of the limiting factor in pea cropping (Martín-Sanz et al. 2011).
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia
IS  - 5
SP  - 724
VL  - 99
DO  - 10.1094/PDIS-11-14-1212-PDN
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Ivanović, Žarko and Trkulja, Nenad and Milosavljević, Anja and Ignjatov, Maja",
year = "2015",
abstract = "In May 2014, during a cool, humid spring period, bacterial spot symptoms were observed for the first time on pea (Pisum sativum L.) in a home garden in the Bačka region of Vojvodina Province in Serbia. Commercial pea is grown on 11,533 ha, as well as in small vegetable farms in Serbia, with average yields of 4 t/ha. Observed spots on leaves were necrotic and progressed inward from the margins. Disease incidence approached 10 to 30%. Bacteria consistently isolated from margins of leaf lesions macerated in sterile phosphate buffered saline (PBS, pH 7.4) formed round, shiny, white, 2- to 3-mm in diameter colonies on nutrient agar with 5% (w/v) sucrose (NAS) and a green fluorescent pigment on King’s medium B (KB). A collection of 20 strains selected for further study was strictly aerobic, gram-negative; positive for levan and tobacco hypersensitive response and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Strains were characterized by repetitive (rep)-PCR, sequence typing, and pathogenicity. To determine genetic heterogenicity among the tested strains, rep-PCR genetic fingerprinting was conducted using the REP, ERIC, and BOX primers (Louws et al. 1994). Profile analyses yielded identical banding patterns for all tested strains. To identify three representative strains by sequence typing, the housekeeping genes gyrB and gltA were amplified (Ferrante and Scortichini 2010). Sequences were deposited in GenBank under accession numbers KP188575 to 77 for gyrB and KP188578 to 80 for gltA. BLAST analysis showed that the regions were 100% homologous for the partial gltA gene of the reference strain P. syringae pv. syringae CFBP 4702 (KF937504) and 99% homologous for the partial gyrB gene of the reference strain P. syringae pv. syringae PD 2021 (KJ158894). Pathogenicity of the three tested strains was tested by using lesion tests and stem inoculation (Mazarei and Kerr 1990). For all tests, a reference strain P. syringae pv. syringae GSPB 1142 and PBS served as a positive and negative controls, respectively. In lesion tests, young bean pods and pale yellowish-green lemon fruits were inoculated by placing drops of bacterial suspension (with 108 CFU/ml) on the surface and pricking lightly through the drops with a sterile needle. Fruits and pods were incubated at 25°C for 7 days. Tested strains produced small reddish-brown depressed lesions on bean pods and dark brown depressed lesions on lemon fruits. For stem inoculation, pea cv. Mali provansalac plantlets were grown from seed for 10 to 14 days. Bacteria grown on KB for 24 h were scraped from the surface with a sterile needle and stabbed into the main stem at the youngest two nodes. Inoculation resulted in extensive necrosis and collapse with stunting on the plants 10 days after inoculation. Control strain GSPB 1142 developed similar symptoms to those of the strains tested. No symptoms were observed on plants inoculated with PBS. The bacterium was reisolated from lesions of test plants for the tree tested strains and confirmed to be the same bacterium using LOPAT tests and rep-PCR genetic fingerprinting. To our knowledge, this is the first report of P. syringae pv. syringae causing bacterial brown spot of pea in Serbia. The information of this species as a pea pathogen is still limited but is considered to be one of the limiting factor in pea cropping (Martín-Sanz et al. 2011).",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia",
number = "5",
pages = "724",
volume = "99",
doi = "10.1094/PDIS-11-14-1212-PDN"
}

Popović Milovanović, T., Ivanović, Ž., Trkulja, N., Milosavljević, A.,& Ignjatov, M.. (2015). First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease
American Phytopathological Society., 99(5), 724.
https://doi.org/10.1094/PDIS-11-14-1212-PDN

Popović Milovanović T, Ivanović Ž, Trkulja N, Milosavljević A, Ignjatov M. First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease. 2015;99(5):724.
doi:10.1094/PDIS-11-14-1212-PDN .

Popović Milovanović, Tatjana, Ivanović, Žarko, Trkulja, Nenad, Milosavljević, Anja, Ignjatov, Maja, "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia" in Plant Disease, 99, no. 5 (2015):724,
https://doi.org/10.1094/PDIS-11-14-1212-PDN . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Stanisavljević, Rade
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Bosković, Jelena
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/416
AB  - The emergence of Cercospora beticola populations that are resistant to benzimidazoles (MBC) and demethylation-inhibiting fungicides (DMI) has been recently reported in Serbia and has resulted in a reduced efficacy of fungicides in controlling Cercospora leaf spot (CLS). Between 2008 and 2011, using a discriminatory concentration method in sugar beet fields in two separate regions of Serbia, we determined that 93.3%-98.6% of collected C. bed cola isolates were resistant to MBCs, whereas 6.2%-42.4% were resistant to DMI fungicides. At the same localities, field trials were conducted to investigate the impact of resistant C. beticola populations on disease management From the MBC group of fungicides, both thiophanate methyl and carbendazim failed to suppress the spread of CLS at both of the tested localities. Between 2008 and 2010, DMI fungicides expressed moderate efficacy at a South Banat locality (79.8%-84.6%) whether they were applied individually (flutriafol, epoxiconazole) or in combination with MBCs (epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole). The frequency of resistant isolates in these test trials ranged from 6.2% to 10.9%. In 2011, at the same locality, conditions were observed to change in favor of the occurrence of resistant populations, which comprised up to 18.7% of the population, at the expense of DMI efficacy in CLS management At a Srem locality, the frequency of C. beticola isolates that were resistant to DMIs was high during all four years of field testing (30.5%-42.4%), and the efficacy of these fungicides ranged from 48.4% to 68.0%. A combination of DMI and a protective chlorothalonil had a stable, moderate impact on disease management regardless of the frequency of DMI resistance, whereas a combination of the cyproconazole DMI with trifloxystrobin from the strobilurin group of fungicides expressed the highest efficacy. High correlation coefficient values (r = 0.87) indicated how strongly the frequencies of resistant populations affected disease severity in the trial plots that were treated with carbendazim and thiophanate methyl, as well as in the plots that were treated with flutriafol and epoxiconazole (r = 0.98). In shift sensitivity trials, MBC-resistant C. beticola isolates were found at equally high frequencies both before and after the treatments, indicating a complete loss of efficacy in CLS control. This test revealed the significant impact of multiple DMI applications in terms of increasing the frequency of resistant C. beticola populations following treatments with flutriafol, epoxiconazole, epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole and flutriafol/chlorothalonil, except when used in combination with trifloxystrobin, in which case CLS suppression was substantially high. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management
EP  - 87
SP  - 80
VL  - 75
DO  - 10.1016/j.cropro.2015.05.017
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Stanisavljević, Rade and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Bosković, Jelena",
year = "2015",
abstract = "The emergence of Cercospora beticola populations that are resistant to benzimidazoles (MBC) and demethylation-inhibiting fungicides (DMI) has been recently reported in Serbia and has resulted in a reduced efficacy of fungicides in controlling Cercospora leaf spot (CLS). Between 2008 and 2011, using a discriminatory concentration method in sugar beet fields in two separate regions of Serbia, we determined that 93.3%-98.6% of collected C. bed cola isolates were resistant to MBCs, whereas 6.2%-42.4% were resistant to DMI fungicides. At the same localities, field trials were conducted to investigate the impact of resistant C. beticola populations on disease management From the MBC group of fungicides, both thiophanate methyl and carbendazim failed to suppress the spread of CLS at both of the tested localities. Between 2008 and 2010, DMI fungicides expressed moderate efficacy at a South Banat locality (79.8%-84.6%) whether they were applied individually (flutriafol, epoxiconazole) or in combination with MBCs (epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole). The frequency of resistant isolates in these test trials ranged from 6.2% to 10.9%. In 2011, at the same locality, conditions were observed to change in favor of the occurrence of resistant populations, which comprised up to 18.7% of the population, at the expense of DMI efficacy in CLS management At a Srem locality, the frequency of C. beticola isolates that were resistant to DMIs was high during all four years of field testing (30.5%-42.4%), and the efficacy of these fungicides ranged from 48.4% to 68.0%. A combination of DMI and a protective chlorothalonil had a stable, moderate impact on disease management regardless of the frequency of DMI resistance, whereas a combination of the cyproconazole DMI with trifloxystrobin from the strobilurin group of fungicides expressed the highest efficacy. High correlation coefficient values (r = 0.87) indicated how strongly the frequencies of resistant populations affected disease severity in the trial plots that were treated with carbendazim and thiophanate methyl, as well as in the plots that were treated with flutriafol and epoxiconazole (r = 0.98). In shift sensitivity trials, MBC-resistant C. beticola isolates were found at equally high frequencies both before and after the treatments, indicating a complete loss of efficacy in CLS control. This test revealed the significant impact of multiple DMI applications in terms of increasing the frequency of resistant C. beticola populations following treatments with flutriafol, epoxiconazole, epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole and flutriafol/chlorothalonil, except when used in combination with trifloxystrobin, in which case CLS suppression was substantially high. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management",
pages = "87-80",
volume = "75",
doi = "10.1016/j.cropro.2015.05.017"
}

Trkulja, N., Milosavljević, A., Stanisavljević, R., Mitrović, M., Jović, J., Toševski, I.,& Bosković, J.. (2015). Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management. in Crop Protection
Elsevier Sci Ltd, Oxford., 75, 80-87.
https://doi.org/10.1016/j.cropro.2015.05.017

Trkulja N, Milosavljević A, Stanisavljević R, Mitrović M, Jović J, Toševski I, Bosković J. Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management. in Crop Protection. 2015;75:80-87.
doi:10.1016/j.cropro.2015.05.017 .

Trkulja, Nenad, Milosavljević, Anja, Stanisavljević, Rade, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Bosković, Jelena, "Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management" in Crop Protection, 75 (2015):80-87,
https://doi.org/10.1016/j.cropro.2015.05.017 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Živković, Svetlana
AU  - Popović, Tatjana
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/397
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.
EP  - 1036
IS  - 7
SP  - 1035
VL  - 99
DO  - 10.1094/PDIS-10-14-1096-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Živković, Svetlana and Popović, Tatjana and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.",
pages = "1036-1035",
number = "7",
volume = "99",
doi = "10.1094/PDIS-10-14-1096-PDN"
}

Trkulja, N., Milosavljević, A., Živković, S., Popović, T., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(7), 1035-1036.
https://doi.org/10.1094/PDIS-10-14-1096-PDN

Trkulja N, Milosavljević A, Živković S, Popović T, Mitrović M, Jović J, Toševski I. First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease. 2015;99(7):1035-1036.
doi:10.1094/PDIS-10-14-1096-PDN .

Trkulja, Nenad, Milosavljević, Anja, Živković, Svetlana, Popović, Tatjana, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia." in Plant Disease, 99, no. 7 (2015):1035-1036,
https://doi.org/10.1094/PDIS-10-14-1096-PDN . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Trkulja, Nenad
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/398
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.
EP  - 1275
IS  - 9
SP  - 1274
VL  - 99
DO  - 10.1094/PDIS-12-14-1261-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Trkulja, Nenad and Popović, Tatjana and Ivanović, Žarko and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.",
pages = "1275-1274",
number = "9",
volume = "99",
doi = "10.1094/PDIS-12-14-1261-PDN"
}

Milosavljević, A., Trkulja, N., Popović, T., Ivanović, Ž., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(9), 1274-1275.
https://doi.org/10.1094/PDIS-12-14-1261-PDN

Milosavljević A, Trkulja N, Popović T, Ivanović Ž, Mitrović M, Jović J, Toševski I. First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.. in Plant Disease. 2015;99(9):1274-1275.
doi:10.1094/PDIS-12-14-1261-PDN .

Milosavljević, Anja, Trkulja, Nenad, Popović, Tatjana, Ivanović, Žarko, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia." in Plant Disease, 99, no. 9 (2015):1274-1275,
https://doi.org/10.1094/PDIS-12-14-1261-PDN . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/404
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia
EP  - 1645
IS  - 11
SP  - 1645
VL  - 99
DO  - 10.1094/PDIS-03-15-0251-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia",
pages = "1645-1645",
number = "11",
volume = "99",
doi = "10.1094/PDIS-03-15-0251-PDN"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(11), 1645-1645.
https://doi.org/10.1094/PDIS-03-15-0251-PDN

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I. First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia. in Plant Disease. 2015;99(11):1645-1645.
doi:10.1094/PDIS-03-15-0251-PDN .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia" in Plant Disease, 99, no. 11 (2015):1645-1645,
https://doi.org/10.1094/PDIS-03-15-0251-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Ignjatov, Maja
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/415
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia
EP  - 724
IS  - 5
SP  - 724
VL  - 99
DO  - 10.1094/PDIS-11-14-1212-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Trkulja, Nenad and Milosavljević, Anja and Ignjatov, Maja",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia",
pages = "724-724",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1212-PDN"
}

Popović, T., Ivanović, Ž., Trkulja, N., Milosavljević, A.,& Ignjatov, M.. (2015). First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 724-724.
https://doi.org/10.1094/PDIS-11-14-1212-PDN

Popović T, Ivanović Ž, Trkulja N, Milosavljević A, Ignjatov M. First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease. 2015;99(5):724-724.
doi:10.1094/PDIS-11-14-1212-PDN .

Popović, Tatjana, Ivanović, Žarko, Trkulja, Nenad, Milosavljević, Anja, Ignjatov, Maja, "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia" in Plant Disease, 99, no. 5 (2015):724-724,
https://doi.org/10.1094/PDIS-11-14-1212-PDN . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Stojanović, Saša
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/329
AB  - In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum.
AB  - Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Effect of calcium salts on postharvest fungal pathogens in vitro
T1  - In vitro efekat kalcijumovih soli na gljivične skladišne patogene
EP  - 46
IS  - 1
SP  - 40
VL  - 65
DO  - 10.5937/zasbilj1401040S
ER  - 

@article{
author = "Stošić, Stefan and Stojanović, Saša and Milosavljević, Anja and Pfaf-Dolovac, Erika and Živković, Svetlana",
year = "2014",
abstract = "In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum., Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Effect of calcium salts on postharvest fungal pathogens in vitro, In vitro efekat kalcijumovih soli na gljivične skladišne patogene",
pages = "46-40",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401040S"
}

Stošić, S., Stojanović, S., Milosavljević, A., Pfaf-Dolovac, E.,& Živković, S.. (2014). Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 40-46.
https://doi.org/10.5937/zasbilj1401040S

Stošić S, Stojanović S, Milosavljević A, Pfaf-Dolovac E, Živković S. Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja. 2014;65(1):40-46.
doi:10.5937/zasbilj1401040S .

Stošić, Stefan, Stojanović, Saša, Milosavljević, Anja, Pfaf-Dolovac, Erika, Živković, Svetlana, "Effect of calcium salts on postharvest fungal pathogens in vitro" in Zaštita bilja, 65, no. 1 (2014):40-46,
https://doi.org/10.5937/zasbilj1401040S . .

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Kuzmanović, Slobodan
AU  - Aleksić, Goran
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/328
AB  - Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%).
AB  - Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion
T1  - Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije
EP  - 32
IS  - 1
SP  - 27
VL  - 65
DO  - 10.5937/zasbilj1401027S
ER  - 

@article{
author = "Stevanović, Miloš and Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Kuzmanović, Slobodan and Aleksić, Goran",
year = "2014",
abstract = "Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%)., Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion, Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije",
pages = "32-27",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401027S"
}

Stevanović, M., Dolovac, N., Trkulja, N., Milosavljević, A., Kuzmanović, S.,& Aleksić, G.. (2014). Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 27-32.
https://doi.org/10.5937/zasbilj1401027S

Stevanović M, Dolovac N, Trkulja N, Milosavljević A, Kuzmanović S, Aleksić G. Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja. 2014;65(1):27-32.
doi:10.5937/zasbilj1401027S .

Stevanović, Miloš, Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Kuzmanović, Slobodan, Aleksić, Goran, "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion" in Zaštita bilja, 65, no. 1 (2014):27-32,
https://doi.org/10.5937/zasbilj1401027S . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/325
AB  - C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank.
AB  - C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and molecular identification of Cercospora apii on celery in Serbia
T1  - Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji
EP  - 84
IS  - 2
SP  - 77
VL  - 65
DO  - 10.5937/zasbilj1402077M
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
abstract = "C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank., C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and molecular identification of Cercospora apii on celery in Serbia, Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji",
pages = "84-77",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402077M"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(2), 77-84.
https://doi.org/10.5937/zasbilj1402077M

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja. 2014;65(2):77-84.
doi:10.5937/zasbilj1402077M .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "Morphological and molecular identification of Cercospora apii on celery in Serbia" in Zaštita bilja, 65, no. 2 (2014):77-84,
https://doi.org/10.5937/zasbilj1402077M . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/340
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia
EP  - 1157
IS  - 8
SP  - 1157
VL  - 98
DO  - 10.1094/PDIS-02-14-0135-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia",
pages = "1157-1157",
number = "8",
volume = "98",
doi = "10.1094/PDIS-02-14-0135-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1157-1157.
https://doi.org/10.1094/PDIS-02-14-0135-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease. 2014;98(8):1157-1157.
doi:10.1094/PDIS-02-14-0135-PDN .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia" in Plant Disease, 98, no. 8 (2014):1157-1157,
https://doi.org/10.1094/PDIS-02-14-0135-PDN . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/341
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.
EP  - 1153
IS  - 8
SP  - 1153
VL  - 98
DO  - 10.1094/PDIS-08-13-0858-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.",
pages = "1153-1153",
number = "8",
volume = "98",
doi = "10.1094/PDIS-08-13-0858-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1153-1153.
https://doi.org/10.1094/PDIS-08-13-0858-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease. 2014;98(8):1153-1153.
doi:10.1094/PDIS-08-13-0858-PDN .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia." in Plant Disease, 98, no. 8 (2014):1153-1153,
https://doi.org/10.1094/PDIS-08-13-0858-PDN . .

TY  - CONF
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/334
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola
EP  - 120
IS  - 11
SP  - 120
VL  - 104
ER  - 

@conference{
author = "Trkulja, Nenad and Dolovac, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola",
pages = "120-120",
number = "11",
volume = "104"
}

Trkulja, N., Dolovac, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 120-120.

Trkulja N, Dolovac N, Milosavljević A, Pfaf-Dolovac E. Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology. 2014;104(11):120-120..

Trkulja, Nenad, Dolovac, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola" in Phytopathology, 104, no. 11 (2014):120-120.

TY  - CONF
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/335
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management
EP  - 34
IS  - 11
SP  - 34
VL  - 104
ER  - 

@conference{
author = "Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management",
pages = "34-34",
number = "11",
volume = "104"
}

Dolovac, N., Trkulja, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 34-34.

Dolovac N, Trkulja N, Milosavljević A, Pfaf-Dolovac E. Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology. 2014;104(11):34-34..

Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management" in Phytopathology, 104, no. 11 (2014):34-34.

TY  - JOUR
AU  - Đukanović, Lana
AU  - Vuga-Janjatov, Vesna
AU  - Vrhovac, Ivana
AU  - Milosavljević, Anja
AU  - Poštić, Dobrivoj
AU  - Mitrović, Milana
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/234
AB  - Investigation of the effect Alternaria alternata on seed germination of wheat were tested on three varieties of Victory, Renaissance and NS 40 S. Seed germination is the ISTA rules, in order to elucidate the effect of primary and secondary infection pathogen A.alternata on seed natural and conditioned seeds were placed tests with seeds untreated, treated with NaOCl and fungicide Tycoon included. The high rate of seed germination was found in all three experiments. The correlation coefficient between secondary infection of seedlings with A.alternata and germination of wheat was -0.69 to -0.76, depending on the variety, indicating that the rate of infection has no significant effect on seed germination.
AB  - Ispitivanje uticaja gljive Alternaria alternata na klijavost semena pšenice testirano je na tri sorte Pobeda, Renesansa i NS 40 S. Prema ISTA pravilima, a u cilju utvrđivanja uticaja primarne i sekundarne infekcije patogena A.alternata na klijavost naturalnog i dorađenog semenskog materijala, postavljeni su testovi sa semenom bez tretiranja, tretirano sa NaOCl i fungicidom Tycoon-om. Visoka stopa klijavosti semena je utvrđena u sva tri eksperimenta. Koeficijent korelacije između sekundarne zaraze klijanaca sa A.alternata i klijavosti semena pšenice iznosio je -0.69 do -0.76 u zavisnosti od sorte, ukazujući da infekcija nema značajan uticaj na klijavost semena.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Impact of Alternaria alternata on seed germination wheat
T1  - Uticaj Alternaria alternata na klijavost semena pšenice
EP  - 197
IS  - 4
SP  - 192
VL  - 63
ER  - 

@article{
author = "Đukanović, Lana and Vuga-Janjatov, Vesna and Vrhovac, Ivana and Milosavljević, Anja and Poštić, Dobrivoj and Mitrović, Milana and Trkulja, Nenad",
year = "2012",
abstract = "Investigation of the effect Alternaria alternata on seed germination of wheat were tested on three varieties of Victory, Renaissance and NS 40 S. Seed germination is the ISTA rules, in order to elucidate the effect of primary and secondary infection pathogen A.alternata on seed natural and conditioned seeds were placed tests with seeds untreated, treated with NaOCl and fungicide Tycoon included. The high rate of seed germination was found in all three experiments. The correlation coefficient between secondary infection of seedlings with A.alternata and germination of wheat was -0.69 to -0.76, depending on the variety, indicating that the rate of infection has no significant effect on seed germination., Ispitivanje uticaja gljive Alternaria alternata na klijavost semena pšenice testirano je na tri sorte Pobeda, Renesansa i NS 40 S. Prema ISTA pravilima, a u cilju utvrđivanja uticaja primarne i sekundarne infekcije patogena A.alternata na klijavost naturalnog i dorađenog semenskog materijala, postavljeni su testovi sa semenom bez tretiranja, tretirano sa NaOCl i fungicidom Tycoon-om. Visoka stopa klijavosti semena je utvrđena u sva tri eksperimenta. Koeficijent korelacije između sekundarne zaraze klijanaca sa A.alternata i klijavosti semena pšenice iznosio je -0.69 do -0.76 u zavisnosti od sorte, ukazujući da infekcija nema značajan uticaj na klijavost semena.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Impact of Alternaria alternata on seed germination wheat, Uticaj Alternaria alternata na klijavost semena pšenice",
pages = "197-192",
number = "4",
volume = "63"
}

Đukanović, L., Vuga-Janjatov, V., Vrhovac, I., Milosavljević, A., Poštić, D., Mitrović, M.,& Trkulja, N.. (2012). Impact of Alternaria alternata on seed germination wheat. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(4), 192-197.

Đukanović L, Vuga-Janjatov V, Vrhovac I, Milosavljević A, Poštić D, Mitrović M, Trkulja N. Impact of Alternaria alternata on seed germination wheat. in Zaštita bilja. 2012;63(4):192-197..

Đukanović, Lana, Vuga-Janjatov, Vesna, Vrhovac, Ivana, Milosavljević, Anja, Poštić, Dobrivoj, Mitrović, Milana, Trkulja, Nenad, "Impact of Alternaria alternata on seed germination wheat" in Zaštita bilja, 63, no. 4 (2012):192-197.

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Stevanović, Miloš
AU  - Popović, Tatjana
AU  - Đukanović, Lana
AU  - Živković, Svetlana
AU  - Mitrović, Milana
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/240
AB  - Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium.
AB  - Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits
T1  - Morfološke i odgajivačke karakteristike izolata Monilinia laxa sa koštičavih voćaka
EP  - 158
IS  - 3
SP  - 148
VL  - 63
ER  - 

@article{
author = "Milosavljević, Anja and Stevanović, Miloš and Popović, Tatjana and Đukanović, Lana and Živković, Svetlana and Mitrović, Milana and Trkulja, Nenad",
year = "2012",
abstract = "Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium., Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits, Morfološke i odgajivačke karakteristike izolata Monilinia laxa sa koštičavih voćaka",
pages = "158-148",
number = "3",
volume = "63"
}

Milosavljević, A., Stevanović, M., Popović, T., Đukanović, L., Živković, S., Mitrović, M.,& Trkulja, N.. (2012). Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 148-158.

Milosavljević A, Stevanović M, Popović T, Đukanović L, Živković S, Mitrović M, Trkulja N. Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits. in Zaštita bilja. 2012;63(3):148-158..

Milosavljević, Anja, Stevanović, Miloš, Popović, Tatjana, Đukanović, Lana, Živković, Svetlana, Mitrović, Milana, Trkulja, Nenad, "Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits" in Zaštita bilja, 63, no. 3 (2012):148-158.

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Blagojević, Jovana
AU  - Ivanović, Žarko
AU  - Milosavljević, Anja
AU  - Popović, Tatjana
AU  - Kuzmanović, Slobodan
AU  - Bošković, Jelena
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/235
AB  - Determination of breeding and morphological characteristics of C. beticola isolates was performed on PDA, MEA i CDA mediums. Determination of morphological characteristics were evaluated by monitoring mycelial growth, texture, color, edge and color of beneath of mycelium. Isolates growth on different temperatures was monitored on PDA medium on 10°C, 15°C, 20°C, 25°C, 30°C, 35°C and 40°C. There was a significant increase in variability of the isolates on PDA, MEA and CDA mediums, but there is a variability in the growth of different isolates of the same medium too. Isolates expressed the variation in color and the width of the edge zone during growth on different substrates. The mycelium texture was cottony on all surfaces, while the beneath was brown to dark-olive colour. At temperatures of 10°C and 40°C isolates of C. beticola did not develop mycelium, and the highest increase was recorded at 25°C.
AB  - Utvrđivanje morfoloških i odgajivačkih karakteristika izolata C. beticola izvedeno je na KDA, MEA i CDA podlozi. Utvrđivanje morfoloških karakteristika obuhvatilo je praćenje porasta micelije, teksture, boje, ivične zone i boje naličja micelije. Porast izolata na različitim temperaturama praćen je na KDA podlozi na temperaturama 10°C, 15°C, 20°C, 25°C, 30°C, 35°C i 40°C. Utvrđena je značajna varijabilnost porasta izolata na KDA, MEA i CDA podlozi, a variabilnost postoji i u porastu različitih izolata na istoj podlozi. Izolati su ispoljili i variranje u boji i širini ivične zone tokom porasta na različitim podlogama. Tekstura micelije bila je pamučasta na svim podlogama, dok je boja sa naličja bila mrka do tamno-maslinasta. Na temperaturama 10°C i 40°C izolati C. beticola nisu razvijali miceliju, a najintenzivniji porast je ostvaren na 25°C.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and breeding characteristics of Cercospora beticola isolates
T1  - Morfološke i odgajivačke karakteristike izolata Cercospora beticola
EP  - 52
IS  - 1
SP  - 45
VL  - 63
ER  - 

@article{
author = "Trkulja, Nenad and Blagojević, Jovana and Ivanović, Žarko and Milosavljević, Anja and Popović, Tatjana and Kuzmanović, Slobodan and Bošković, Jelena",
year = "2012",
abstract = "Determination of breeding and morphological characteristics of C. beticola isolates was performed on PDA, MEA i CDA mediums. Determination of morphological characteristics were evaluated by monitoring mycelial growth, texture, color, edge and color of beneath of mycelium. Isolates growth on different temperatures was monitored on PDA medium on 10°C, 15°C, 20°C, 25°C, 30°C, 35°C and 40°C. There was a significant increase in variability of the isolates on PDA, MEA and CDA mediums, but there is a variability in the growth of different isolates of the same medium too. Isolates expressed the variation in color and the width of the edge zone during growth on different substrates. The mycelium texture was cottony on all surfaces, while the beneath was brown to dark-olive colour. At temperatures of 10°C and 40°C isolates of C. beticola did not develop mycelium, and the highest increase was recorded at 25°C., Utvrđivanje morfoloških i odgajivačkih karakteristika izolata C. beticola izvedeno je na KDA, MEA i CDA podlozi. Utvrđivanje morfoloških karakteristika obuhvatilo je praćenje porasta micelije, teksture, boje, ivične zone i boje naličja micelije. Porast izolata na različitim temperaturama praćen je na KDA podlozi na temperaturama 10°C, 15°C, 20°C, 25°C, 30°C, 35°C i 40°C. Utvrđena je značajna varijabilnost porasta izolata na KDA, MEA i CDA podlozi, a variabilnost postoji i u porastu različitih izolata na istoj podlozi. Izolati su ispoljili i variranje u boji i širini ivične zone tokom porasta na različitim podlogama. Tekstura micelije bila je pamučasta na svim podlogama, dok je boja sa naličja bila mrka do tamno-maslinasta. Na temperaturama 10°C i 40°C izolati C. beticola nisu razvijali miceliju, a najintenzivniji porast je ostvaren na 25°C.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and breeding characteristics of Cercospora beticola isolates, Morfološke i odgajivačke karakteristike izolata Cercospora beticola",
pages = "52-45",
number = "1",
volume = "63"
}

Trkulja, N., Blagojević, J., Ivanović, Ž., Milosavljević, A., Popović, T., Kuzmanović, S.,& Bošković, J.. (2012). Morphological and breeding characteristics of Cercospora beticola isolates. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(1), 45-52.

Trkulja N, Blagojević J, Ivanović Ž, Milosavljević A, Popović T, Kuzmanović S, Bošković J. Morphological and breeding characteristics of Cercospora beticola isolates. in Zaštita bilja. 2012;63(1):45-52..

Trkulja, Nenad, Blagojević, Jovana, Ivanović, Žarko, Milosavljević, Anja, Popović, Tatjana, Kuzmanović, Slobodan, Bošković, Jelena, "Morphological and breeding characteristics of Cercospora beticola isolates" in Zaštita bilja, 63, no. 1 (2012):45-52.