Ignjatov, Maja

Link to this page

Authority KeyName Variants
orcid::0000-0003-4650-5082
  • Ignjatov, Maja (51)
Projects

Author's Bibliography

Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.

Ignjatov, Maja; Milošević, Dragana; Aćimović, Milica; Medic-Pap, Sladjana; Ivanović, Žarko

(Matica srpska, 2022-06)

TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Aćimović, Milica
AU  - Medic-Pap, Sladjana
AU  - Ivanović, Žarko
PY  - 2022-06
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/767
AB  - Hyssop (Hyssopus officinalis L.) is a perennial polymorphous plant species with essential oil
mainly accumulated in the flowers and leaves. It is grown in Serbia for the needs of
pharmaceutical companies and tea production, because of its quality and chemical
composition. During a routine quality control of hyssop seeds collected from Rumenka
(Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an
average of 22%. Infected seeds were covered with white mycelium followed with violet
pigmentation occurring under the seeds. The presence of Fusarium spp. was confirmed with
microscopic observation. Isolation was done aseptically by arranging infected seeds onto
surface of potato dextrose agar (PDA), and incubated at 25 °C with a 12-h photoperiod
(Mathur and Kongsdall, 2003). After seven days, 12 Fusarium spp. isolates were designated
as JBL 4003/1 - 4003/12. Pathogenicity test was performed in vitro using a modified agar
slant method in the test tube with PDA amended. After 10 days, fungal mycelia of tested
isolates caused seed rot and seedling decay, like naturally infected hyssop seeds. All isolates
were re-isolated and sub-cultured on Potato Dextrose Agar (PDA) and Carnation Leaf Agar
(CLA) using a hyphal tip transfer technique, fulfilling Koch's postulates. Isolate JBL 4003/1
was distinguished based on pathogenicity and cultural characteristics. It caused seed rot after
four days, on PDA colony was fast growing reaching 6-8 cm in diam. in five days, forming
abundant, whitish to peach aerial mycelium followed with beige to light brown pigmentation
in agar. Isolate formed relatively long and narrow macroconidia (24 to 54 × 3.2 to 4.5 μm)
with a tapered and elongated apical cell and prominent foot-shaped basal cell, with four to six
septate, with no microconidia. Chlamydospores were solitary and intercalary. Based cultural
and morphological characteristics indicated that the isolate belong to species Fusarium
equiseti Corda (Saccardo). To obtain a DNA sequence-based identification, total DNA was
extracted directly from the mycelium. Following DNA extraction, the translation elongation
factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2. The
amplified and purified DNA fragment of chosen isolate JBL4003/1 was sequenced in both
directions and deposited in the GeneBank under Accession Number MK061540.1. BLAST
analysis revealed that the Serbian isolate MK061540 showed the highest nucleotide identity
of 100% with F. equiseti isolates from United States (MG826890), Canada (KU587617),
Turkey (KT286761), and Serbia (JQ412101). Based on morphological and pathogenic
properties, as well as the sequence analysis, to our knowledge, this is the first case of F.
equiseti Corda (Saccardo) as the causal agent of Hyssopus officinalis (L.) seed rot in Serbia.
Considering the importance of H. Officinalis in pharmaceutical industries, knowledge of the
composition of populations of Fusarium species transmitted by hyssop.
PB  - Matica srpska
C3  - Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022
T1  - Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.
EP  - 67
SP  - 67
ER  - 
@conference{
author = "Ignjatov, Maja and Milošević, Dragana and Aćimović, Milica and Medic-Pap, Sladjana and Ivanović, Žarko",
year = "2022-06",
abstract = "Hyssop (Hyssopus officinalis L.) is a perennial polymorphous plant species with essential oil
mainly accumulated in the flowers and leaves. It is grown in Serbia for the needs of
pharmaceutical companies and tea production, because of its quality and chemical
composition. During a routine quality control of hyssop seeds collected from Rumenka
(Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an
average of 22%. Infected seeds were covered with white mycelium followed with violet
pigmentation occurring under the seeds. The presence of Fusarium spp. was confirmed with
microscopic observation. Isolation was done aseptically by arranging infected seeds onto
surface of potato dextrose agar (PDA), and incubated at 25 °C with a 12-h photoperiod
(Mathur and Kongsdall, 2003). After seven days, 12 Fusarium spp. isolates were designated
as JBL 4003/1 - 4003/12. Pathogenicity test was performed in vitro using a modified agar
slant method in the test tube with PDA amended. After 10 days, fungal mycelia of tested
isolates caused seed rot and seedling decay, like naturally infected hyssop seeds. All isolates
were re-isolated and sub-cultured on Potato Dextrose Agar (PDA) and Carnation Leaf Agar
(CLA) using a hyphal tip transfer technique, fulfilling Koch's postulates. Isolate JBL 4003/1
was distinguished based on pathogenicity and cultural characteristics. It caused seed rot after
four days, on PDA colony was fast growing reaching 6-8 cm in diam. in five days, forming
abundant, whitish to peach aerial mycelium followed with beige to light brown pigmentation
in agar. Isolate formed relatively long and narrow macroconidia (24 to 54 × 3.2 to 4.5 μm)
with a tapered and elongated apical cell and prominent foot-shaped basal cell, with four to six
septate, with no microconidia. Chlamydospores were solitary and intercalary. Based cultural
and morphological characteristics indicated that the isolate belong to species Fusarium
equiseti Corda (Saccardo). To obtain a DNA sequence-based identification, total DNA was
extracted directly from the mycelium. Following DNA extraction, the translation elongation
factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2. The
amplified and purified DNA fragment of chosen isolate JBL4003/1 was sequenced in both
directions and deposited in the GeneBank under Accession Number MK061540.1. BLAST
analysis revealed that the Serbian isolate MK061540 showed the highest nucleotide identity
of 100% with F. equiseti isolates from United States (MG826890), Canada (KU587617),
Turkey (KT286761), and Serbia (JQ412101). Based on morphological and pathogenic
properties, as well as the sequence analysis, to our knowledge, this is the first case of F.
equiseti Corda (Saccardo) as the causal agent of Hyssopus officinalis (L.) seed rot in Serbia.
Considering the importance of H. Officinalis in pharmaceutical industries, knowledge of the
composition of populations of Fusarium species transmitted by hyssop.",
publisher = "Matica srpska",
journal = "Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022",
title = "Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.",
pages = "67-67"
}
Ignjatov, M., Milošević, D., Aćimović, M., Medic-Pap, S.,& Ivanović, Ž.. (2022-06). Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022
Matica srpska., 67-67.
Ignjatov M, Milošević D, Aćimović M, Medic-Pap S, Ivanović Ž. Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L.. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022. 2022;:67-67..
Ignjatov, Maja, Milošević, Dragana, Aćimović, Milica, Medic-Pap, Sladjana, Ivanović, Žarko, "Occurence of Fusarium equiseti Corda (Saccardo) as causal agent of seed rot of Hyssopus officinalis L." in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022 (2022-06):67-67.

First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia

Loc, Marta; Milošević, Dragana; Ignjatov, Maja; Ivanović, Žarko; Budakov, Dragana; Grahovac, Jovana; Vlajkov, Vanja; Pajčin, Ivana; Grahovac, Mila

(American Phytopathological Society, 2022)

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1144
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 
@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}
Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN
Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .
Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .
9
8

Genetic Diversity of Pectobacterium spp. on Potato in Serbia

Loc, Marta; Milošević, Dragana; Ivanović, Žarko; Ignjatov, Maja; Budakov, Dragana; Grahovac, Jovana; Grahovac, Mila

(MDPI, 2022)

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/685
AB  - Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Genetic Diversity of Pectobacterium spp. on Potato in Serbia
IS  - 9
VL  - 10
DO  - 10.3390/microorganisms10091840
ER  - 
@article{
author = "Loc, Marta and Milošević, Dragana and Ivanović, Žarko and Ignjatov, Maja and Budakov, Dragana and Grahovac, Jovana and Grahovac, Mila",
year = "2022",
abstract = "Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Genetic Diversity of Pectobacterium spp. on Potato in Serbia",
number = "9",
volume = "10",
doi = "10.3390/microorganisms10091840"
}
Loc, M., Milošević, D., Ivanović, Ž., Ignjatov, M., Budakov, D., Grahovac, J.,& Grahovac, M.. (2022). Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms
MDPI., 10(9).
https://doi.org/10.3390/microorganisms10091840
Loc M, Milošević D, Ivanović Ž, Ignjatov M, Budakov D, Grahovac J, Grahovac M. Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms. 2022;10(9).
doi:10.3390/microorganisms10091840 .
Loc, Marta, Milošević, Dragana, Ivanović, Žarko, Ignjatov, Maja, Budakov, Dragana, Grahovac, Jovana, Grahovac, Mila, "Genetic Diversity of Pectobacterium spp. on Potato in Serbia" in Microorganisms, 10, no. 9 (2022),
https://doi.org/10.3390/microorganisms10091840 . .
3
2

Botrytis cinerea as causal agent of sunflower seed grey mould

Ignjatov, Maja; Milošević, Dragana; Miklic, Vladimir; Dedic, Bosko; Tamindžić, Gordana; Bjelić, Dragana; Ivanović, Žarko

(International Sunflower Association, Institute of Field and Vegetable Crops, 2022)

TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Miklic, Vladimir
AU  - Dedic, Bosko
AU  - Tamindžić, Gordana
AU  - Bjelić, Dragana
AU  - Ivanović, Žarko
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/776
AB  - Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.
PB  - International Sunflower Association, Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad
T1  - Botrytis cinerea as causal agent of sunflower seed grey mould
SP  - 209
ER  - 
@conference{
author = "Ignjatov, Maja and Milošević, Dragana and Miklic, Vladimir and Dedic, Bosko and Tamindžić, Gordana and Bjelić, Dragana and Ivanović, Žarko",
year = "2022",
abstract = "Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.",
publisher = "International Sunflower Association, Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad",
title = "Botrytis cinerea as causal agent of sunflower seed grey mould",
pages = "209"
}
Ignjatov, M., Milošević, D., Miklic, V., Dedic, B., Tamindžić, G., Bjelić, D.,& Ivanović, Ž.. (2022). Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad
International Sunflower Association, Institute of Field and Vegetable Crops., 209.
Ignjatov M, Milošević D, Miklic V, Dedic B, Tamindžić G, Bjelić D, Ivanović Ž. Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad. 2022;:209..
Ignjatov, Maja, Milošević, Dragana, Miklic, Vladimir, Dedic, Bosko, Tamindžić, Gordana, Bjelić, Dragana, Ivanović, Žarko, "Botrytis cinerea as causal agent of sunflower seed grey mould" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad (2022):209.

Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot

Ignjatov, Maja; Milošević, Dragana; Tamindžić, Gordana; Ivanović, Žarko

(Matica Srpska, 2021)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/625
AB  - Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot
EP  - 27
SP  - 21
VL  - 140
DO  - 10.2298/ZMSPN2140021I
ER  - 
@article{
author = "Ignjatov, Maja and Milošević, Dragana and Tamindžić, Gordana and Ivanović, Žarko",
year = "2021",
abstract = "Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot",
pages = "27-21",
volume = "140",
doi = "10.2298/ZMSPN2140021I"
}
Ignjatov, M., Milošević, D., Tamindžić, G.,& Ivanović, Ž.. (2021). Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 21-27.
https://doi.org/10.2298/ZMSPN2140021I
Ignjatov M, Milošević D, Tamindžić G, Ivanović Ž. Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot. in Zbornik Matice srpske za prirodne nauke. 2021;140:21-27.
doi:10.2298/ZMSPN2140021I .
Ignjatov, Maja, Milošević, Dragana, Tamindžić, Gordana, Ivanović, Žarko, "Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot" in Zbornik Matice srpske za prirodne nauke, 140 (2021):21-27,
https://doi.org/10.2298/ZMSPN2140021I . .

Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji

Obradović, Aleksa; Ivanović, Milan; Gašić, Katarina; Grahovac, Mila; Duduk, Bojan; Milijašević-Marčić, Svetlana; Ignjatov, Maja; Šević, Milan

(Društvo za zaštitu bilja Srbije, 2021)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
AU  - Gašić, Katarina
AU  - Grahovac, Mila
AU  - Duduk, Bojan
AU  - Milijašević-Marčić, Svetlana
AU  - Ignjatov, Maja
AU  - Šević, Milan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/812
AB  - Istraživanja akademika Živojina Tešića iz 1934. godine, a zatim i prof. Dragoljuba Šutića
u prvoj deceniji posle drugog svetskog rata, na Poljoprivredno-šumarskom, kasnije
Poljoprivrednom fakultetu u Zemunu, mogu se smatrati početkom proučavanja fitopatogenih
bakterija (FPB) u Srbiji. Od tada do danas istraživači su prošli mukotrpan put tokom kojeg su
razvijali i širili znanja na polju ovog dela fitopatologije. Uvođenjem fitobakteriologije u
nastavu na Poljoprivrednim fakultetima u Novom Sadu i Beogradu, zahvaljujući entuzijazmu i
naučnoj dalekovidosti profesora Momčila Arsenijevića i Milana Panića, ova oblast istraživanja
dobila je na intenzitetu i značaju. Poslednjih decenija razvoj fitobakteriologije tekao je
dinamično i ubrzano, zahvaljujući sve bržem napretku nauke i tehnologije, razvoju sredstava
komunikacije, posvećenosti istraživača, naučnoj saradnji, studijskim boravcima u
međunarodnim obrazovnim i naučnim institucijama, novim metodama izučavanja i
olakšanom pristupu informacijama. U Srbiji, najviše pažnje je posvećeno proučavanju FPB, a
kasnije i fitoplazmi, poreklom iz ekonomski značajnih biljnih vrsta voćaka, vinove loze,
povrtarskih, ratarskih i ukrasnih biljaka. Zahvaljujući interesovanju i entuzijazmu nevelikog
broja istraživača, usvajane su najsavremenije metode istraživanja ovih patogena.
Fitobakteriološka istraživanja su među prvima uvela u primenu molekularne metode u
domaćoj fitopatologiji. Primena PCR metode omogućila je razvoj nove epohe istraživanja
prokariota u Srbiji i upotrebu tehnika kao što su sekvenciranje genoma, analiza specifičnih ili
konstitutivnih gena, kvantitativni ili Real-time PCR, a to su metode koje se danas rutinski
koriste u istrživanjima prokariota u Srbiji. U novijem periodu razvijena su istraživanja u
oblasti detekcije, identifikacije i karakterizacije fitoplazmi u Srbiji, prateći ili predvodeći
svetska saznanja u ovoj oblasti. Primena savremenih metoda istraživanja omogućila je
izučavanje genetske strukture populacija fitopatogenih prokariota, detekciju novih vrsta u
našoj zemlji, stvaranje markera za precizniju identifikaciju ili proširivanje znanja iz oblasti
epidemiologije fitopatogenih prokariota. Osim toga, stečena saznanja se koriste u razvoju
inovativnih mogućnosti zaštite od bakterioza biološkim i konvencionalnim metodama. Decenijama sticano i unapređivano znanje neprekidno se prenosi generacijama koje dolaze,
kroz učešće fitobakteriologa u nastavnom procesu i kroz mentorski rad u visokoškolskim i
naučnim ustanovama, stvarajući tako osnov za dalji razvoj i napredak fitobakteriologije u
Srbiji.
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19
T1  - Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji
ER  - 
@conference{
author = "Obradović, Aleksa and Ivanović, Milan and Gašić, Katarina and Grahovac, Mila and Duduk, Bojan and Milijašević-Marčić, Svetlana and Ignjatov, Maja and Šević, Milan",
year = "2021",
abstract = "Istraživanja akademika Živojina Tešića iz 1934. godine, a zatim i prof. Dragoljuba Šutića
u prvoj deceniji posle drugog svetskog rata, na Poljoprivredno-šumarskom, kasnije
Poljoprivrednom fakultetu u Zemunu, mogu se smatrati početkom proučavanja fitopatogenih
bakterija (FPB) u Srbiji. Od tada do danas istraživači su prošli mukotrpan put tokom kojeg su
razvijali i širili znanja na polju ovog dela fitopatologije. Uvođenjem fitobakteriologije u
nastavu na Poljoprivrednim fakultetima u Novom Sadu i Beogradu, zahvaljujući entuzijazmu i
naučnoj dalekovidosti profesora Momčila Arsenijevića i Milana Panića, ova oblast istraživanja
dobila je na intenzitetu i značaju. Poslednjih decenija razvoj fitobakteriologije tekao je
dinamično i ubrzano, zahvaljujući sve bržem napretku nauke i tehnologije, razvoju sredstava
komunikacije, posvećenosti istraživača, naučnoj saradnji, studijskim boravcima u
međunarodnim obrazovnim i naučnim institucijama, novim metodama izučavanja i
olakšanom pristupu informacijama. U Srbiji, najviše pažnje je posvećeno proučavanju FPB, a
kasnije i fitoplazmi, poreklom iz ekonomski značajnih biljnih vrsta voćaka, vinove loze,
povrtarskih, ratarskih i ukrasnih biljaka. Zahvaljujući interesovanju i entuzijazmu nevelikog
broja istraživača, usvajane su najsavremenije metode istraživanja ovih patogena.
Fitobakteriološka istraživanja su među prvima uvela u primenu molekularne metode u
domaćoj fitopatologiji. Primena PCR metode omogućila je razvoj nove epohe istraživanja
prokariota u Srbiji i upotrebu tehnika kao što su sekvenciranje genoma, analiza specifičnih ili
konstitutivnih gena, kvantitativni ili Real-time PCR, a to su metode koje se danas rutinski
koriste u istrživanjima prokariota u Srbiji. U novijem periodu razvijena su istraživanja u
oblasti detekcije, identifikacije i karakterizacije fitoplazmi u Srbiji, prateći ili predvodeći
svetska saznanja u ovoj oblasti. Primena savremenih metoda istraživanja omogućila je
izučavanje genetske strukture populacija fitopatogenih prokariota, detekciju novih vrsta u
našoj zemlji, stvaranje markera za precizniju identifikaciju ili proširivanje znanja iz oblasti
epidemiologije fitopatogenih prokariota. Osim toga, stečena saznanja se koriste u razvoju
inovativnih mogućnosti zaštite od bakterioza biološkim i konvencionalnim metodama. Decenijama sticano i unapređivano znanje neprekidno se prenosi generacijama koje dolaze,
kroz učešće fitobakteriologa u nastavnom procesu i kroz mentorski rad u visokoškolskim i
naučnim ustanovama, stvarajući tako osnov za dalji razvoj i napredak fitobakteriologije u
Srbiji.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19",
title = "Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji"
}
Obradović, A., Ivanović, M., Gašić, K., Grahovac, M., Duduk, B., Milijašević-Marčić, S., Ignjatov, M.,& Šević, M.. (2021). Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19
Društvo za zaštitu bilja Srbije..
Obradović A, Ivanović M, Gašić K, Grahovac M, Duduk B, Milijašević-Marčić S, Ignjatov M, Šević M. Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19. 2021;..
Obradović, Aleksa, Ivanović, Milan, Gašić, Katarina, Grahovac, Mila, Duduk, Bojan, Milijašević-Marčić, Svetlana, Ignjatov, Maja, Šević, Milan, "Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19 (2021).

First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia

Blagojević, Jovana; Janjatović, S.; Ignjatov, Maja; Trkulja, Nenad; Gašić, Katarina; Ivanović, Žarko

(American Phytopathological Society, 2020)


                                            

                                            
Blagojević, J., Janjatović, S., Ignjatov, M., Trkulja, N., Gašić, K.,& Ivanović, Ž.. (2020). First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia. in Plant Disease
American Phytopathological Society., 104(3), 986.
https://doi.org/10.1094/PDIS-06-19-1335-PDN
Blagojević J, Janjatović S, Ignjatov M, Trkulja N, Gašić K, Ivanović Ž. First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia. in Plant Disease. 2020;104(3):986.
doi:10.1094/PDIS-06-19-1335-PDN .
Blagojević, Jovana, Janjatović, S., Ignjatov, Maja, Trkulja, Nenad, Gašić, Katarina, Ivanović, Žarko, "First Report of a Leaf Spot Disease Caused by Alternaria protenta on the Datura stramonium in Serbia" in Plant Disease, 104, no. 3 (2020):986,
https://doi.org/10.1094/PDIS-06-19-1335-PDN . .
3
2

First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia

Ivanović, Žarko; Milošević, Dragana; Ignjatov, Maja; Marjanovic Jeromela, Ana; Karaman, Maja; Grahovac, Mila

(American Phytopathological Society, 2020)


                                            

                                            
Ivanović, Ž., Milošević, D., Ignjatov, M., Marjanovic Jeromela, A., Karaman, M.,& Grahovac, M.. (2020). First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease
American Phytopathological Society., 104(9), 2516-2516.
https://doi.org/10.1094/PDIS-03-20-0602-PDN
Ivanović Ž, Milošević D, Ignjatov M, Marjanovic Jeromela A, Karaman M, Grahovac M. First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease. 2020;104(9):2516-2516.
doi:10.1094/PDIS-03-20-0602-PDN .
Ivanović, Žarko, Milošević, Dragana, Ignjatov, Maja, Marjanovic Jeromela, Ana, Karaman, Maja, Grahovac, Mila, "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia" in Plant Disease, 104, no. 9 (2020):2516-2516,
https://doi.org/10.1094/PDIS-03-20-0602-PDN . .
2
2

First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia

Ignjatov, Maja; Milošević, Dragana; Nikolić, Zorica; Tamindžić, Gordana; Stojanović, Milan; Popović, Vera; Ivanović, Žarko

(2020)


                                            

                                            
Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Stojanović, M., Popović, V.,& Ivanović, Ž.. (2020). First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease, 104(6), 1864-1864.
https://doi.org/10.1094/PDIS-10-19-2218-PDN
Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Stojanović M, Popović V, Ivanović Ž. First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease. 2020;104(6):1864-1864.
doi:10.1094/PDIS-10-19-2218-PDN .
Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Stojanović, Milan, Popović, Vera, Ivanović, Žarko, "First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia" in Plant Disease, 104, no. 6 (2020):1864-1864,
https://doi.org/10.1094/PDIS-10-19-2218-PDN . .
1

Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L

Ignjatov, Maja; Vlajić, Slobodan; Milošević, Dragana; Nikolić, Zorica; Tamindžić, Gordana; Gvozdanović-Varga, Jelica; Ivanović, Žarko

(Matica srpska, Novi Sad, 2019)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/608
AB  - Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.
AB  - Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L
T1  - Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L
IS  - 137
SP  - 49
DO  - 10.2298/ZMSPN1937049I
ER  - 
@article{
author = "Ignjatov, Maja and Vlajić, Slobodan and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2019",
abstract = "Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves., Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L, Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L",
number = "137",
pages = "49",
doi = "10.2298/ZMSPN1937049I"
}
Ignjatov, M., Vlajić, S., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2019). Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(137), 49.
https://doi.org/10.2298/ZMSPN1937049I
Ignjatov M, Vlajić S, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke. 2019;(137):49.
doi:10.2298/ZMSPN1937049I .
Ignjatov, Maja, Vlajić, Slobodan, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L" in Zbornik Matice srpske za prirodne nauke, no. 137 (2019):49,
https://doi.org/10.2298/ZMSPN1937049I . .
1

Integration of biological and conventional treatments in control of pepper bacterial spot

Šević, Milan; Gašić, Katarina; Ignjatov, Maja; Mijatović, Mirjana; Prokić, Anđelka; Obradović, Aleksa

(Elsevier, 2019)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/576
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}
Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006
Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .
Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .
18
5
14

Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia

Ignjatov, Maja; Milosević, Dragana; Ivanović, Žarko; Karaman, Maja; Vlajić, Slobodan; Nikolić, Zorica; Gvozdanović-Varga, Jelica

(Institute of field and vegetable crops, Novi Sad, 2018)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, Dragana
AU  - Ivanović, Žarko
AU  - Karaman, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/541
AB  - During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank.
AB  - Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia
T1  - Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji
EP  - 129
IS  - 3
SP  - 125
VL  - 55
DO  - 10.5937/RatPov1803125I
ER  - 
@article{
author = "Ignjatov, Maja and Milosević, Dragana and Ivanović, Žarko and Karaman, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica",
year = "2018",
abstract = "During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank., Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia, Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji",
pages = "129-125",
number = "3",
volume = "55",
doi = "10.5937/RatPov1803125I"
}
Ignjatov, M., Milosević, D., Ivanović, Ž., Karaman, M., Vlajić, S., Nikolić, Z.,& Gvozdanović-Varga, J.. (2018). Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 55(3), 125-129.
https://doi.org/10.5937/RatPov1803125I
Ignjatov M, Milosević D, Ivanović Ž, Karaman M, Vlajić S, Nikolić Z, Gvozdanović-Varga J. Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo. 2018;55(3):125-129.
doi:10.5937/RatPov1803125I .
Ignjatov, Maja, Milosević, Dragana, Ivanović, Žarko, Karaman, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia" in Ratarstvo i povrtarstvo, 55, no. 3 (2018):125-129,
https://doi.org/10.5937/RatPov1803125I . .
6

First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia

Ignjatov, Maja; Bjelić, Dragana; Nikolić, Zorica; Milošević, Dragana; Gvozdanović-Varga, Jelica; Marinković, Jelena; Ivanović, Žarko

(American Phytopathological Society, 2017)


                                            

                                            
Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Gvozdanović-Varga, J., Marinković, J.,& Ivanović, Ž.. (2017). First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease
American Phytopathological Society., 101(6), 1047-1048.
https://doi.org/10.1094/PDIS-11-16-1625-PDN
Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Gvozdanović-Varga J, Marinković J, Ivanović Ž. First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease. 2017;101(6):1047-1048.
doi:10.1094/PDIS-11-16-1625-PDN .
Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Gvozdanović-Varga, Jelica, Marinković, Jelena, Ivanović, Žarko, "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia" in Plant Disease, 101, no. 6 (2017):1047-1048,
https://doi.org/10.1094/PDIS-11-16-1625-PDN . .
4
3
5

First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia

Blagojević, Jovana; Ivanović, Žarko; Popović Milovanović, Tatjana; Ignjatov, Maja

(American Phytopathological Society, 2017)


                                            

                                            
Blagojević, J., Ivanović, Ž., Popović Milovanović, T.,& Ignjatov, M.. (2017). First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease
American Phytopathological Society., 101(2), 384-385.
https://doi.org/10.1094/PDIS-08-16-1106-PDN
Blagojević J, Ivanović Ž, Popović Milovanović T, Ignjatov M. First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease. 2017;101(2):384-385.
doi:10.1094/PDIS-08-16-1106-PDN .
Blagojević, Jovana, Ivanović, Žarko, Popović Milovanović, Tatjana, Ignjatov, Maja, "First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia" in Plant Disease, 101, no. 2 (2017):384-385,
https://doi.org/10.1094/PDIS-08-16-1106-PDN . .
1
2
1

Antifungal activity of indigenous Bacillus spp. isolated from soil

Bjelić, Dragana; Ignjatov, Maja; Marinković, Jelena; Spremo, Nemanja; Karaman, Maja; Nikolić, Zorica; Ivanović, Žarko

(Matica Srpska, 2017)

TY  - JOUR
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Marinković, Jelena
AU  - Spremo, Nemanja
AU  - Karaman, Maja
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1044
AB  - Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of indigenous Bacillus spp. isolated from soil
EP  - 269
SP  - 261
VL  - 133
DO  - 10.2298/ZMSPN1733261B
ER  - 
@article{
author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Spremo, Nemanja and Karaman, Maja and Nikolić, Zorica and Ivanović, Žarko",
year = "2017",
abstract = "Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of indigenous Bacillus spp. isolated from soil",
pages = "269-261",
volume = "133",
doi = "10.2298/ZMSPN1733261B"
}
Bjelić, D., Ignjatov, M., Marinković, J., Spremo, N., Karaman, M., Nikolić, Z.,& Ivanović, Ž.. (2017). Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 261-269.
https://doi.org/10.2298/ZMSPN1733261B
Bjelić D, Ignjatov M, Marinković J, Spremo N, Karaman M, Nikolić Z, Ivanović Ž. Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke. 2017;133:261-269.
doi:10.2298/ZMSPN1733261B .
Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Spremo, Nemanja, Karaman, Maja, Nikolić, Zorica, Ivanović, Žarko, "Antifungal activity of indigenous Bacillus spp. isolated from soil" in Zbornik Matice srpske za prirodne nauke, 133 (2017):261-269,
https://doi.org/10.2298/ZMSPN1733261B . .
2

Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia

Ignjatov, Maja; Bjelić, Dragana; Nikolić, Zorica; Milošević, Dragana; Marinković, Jelena; Ivanović, Žarko; Gvozdanović-Varga, Jelica

(Matica Srpska, 2017)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 
@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}
Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I
Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .
Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .
1

Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)

Ignjatov, Maja; Popović Milovanović, Tatjana; Milošević, Dragana; Nikolić, Zorica; Petrović, Gordana; Gvozdanović-Varga, Jelica; Ivanović, Žarko

(Matica Srpska, 2017)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović Milovanović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1026
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
EP  - 17
SP  - 9
VL  - 132
DO  - 10.2298/ZMSPN1732009I
DO  - 0352-4906
ER  - 
@article{
author = "Ignjatov, Maja and Popović Milovanović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)",
pages = "17-9",
volume = "132",
doi = "10.2298/ZMSPN1732009I, 0352-4906"
}
Ignjatov, M., Popović Milovanović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 132, 9-17.
https://doi.org/10.2298/ZMSPN1732009I
Ignjatov M, Popović Milovanović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;132:9-17.
doi:10.2298/ZMSPN1732009I .
Ignjatov, Maja, Popović Milovanović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .
1

Control of pepper and tomato bacterial spot

Šević, Milan; Gašić, Katarina; Ignjatov, Maja; Obradović, Aleksa

(Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2017)

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/505
AB  - Bacterial spot of pepper and tomato caused by Xanthomonas species, is one of the widespread and economically most important diseases of pepper and tomato. Due to the lack of resistant pepper and tomato genotypes, the emergence of new races of bacteria, and strains resistant to copper compounds, questionable seed quality and limited control practices, thesebacteria are limiting pepper production in Serbia. On tomato plants, under favourable weather conditions for disease development, these bacteria can cause major damage reflected in overall yield as well as in fruit quality. The protection of pepper and tomato, based on chemical methods such as use of copper-based compounds in combination with ethylene-bis-dithiocarbamates, do not provide a satisfactory disease control. Use of antibiotics in plant protection is not permitted in the EU as well as in Serbia. However, results of many authors show that satisfactory control of bacterial spot can be achieved by integrating positive effect of chemical treatments (copper compounds, systemic resistance activators) and various biological methods (antagonists, bacteriophages). However, nature of biological agents, as well as the specific mechanism of action of the systemic resistance activators, require careful optimization of the time and number of treatments in order to achieve maximum efficiency.
AB  - Bakteriozna pegavost lista i krastavost plodova prouzrokovana Xanthomonas vrstama, je jedna od najrasprostranjenijih i ekonomski najznačajnijih bolesti paprike i paradajza u svetu. Usled nedostatka otpornih genotipova paprike i paradajza, pojave novih rasa bakterije, sojeva rezistentnih prema jedinjenjima bakra, sumnjivog kvaliteta semena i ograničenih mera kontrole, ova bakterioza predstavlja ograničavajući faktor proizvodnje paprike u Srbiji. Kada vremenski uslovi pogoduju razvoju bolesti na biljkama paradajza, ova bakterioza može prouzrokovati velike gubitke usled smanjenja ukupnog prinosa i kvaliteta plodova koji nisu pogodni za industrijsku preradu. U zaštiti paprike i paradajza kod nas dominiraju hemijske mere borbe, odnosno korišćenje preparata na bazi jedinjenja bakra u kombinaciji sa etilenbis-ditiokarbamatima koji ne obezbeđuju zadovoljavajući efekat zaštite. Antibiotici se ne mogu primenjivati usled zakonskih ograničenja u Evropskoj uniji. Integracijom pozitivnog efekta hemijskih metoda (bakarni preparati, aktivatori sistemične otpornosti) i različitih bioloških metoda (antagonostički sojevi bakterija, bakteriofagi) može se postići zadovoljavajući efekat zaštite. Priroda bioloških agenasa, kao i specifičan mehanizam dejstva aktivatora sistemične otpornosti, zahtevaju pažljivu optimizaciju vremena i broja tretmana kako bi se postigla maksimalna efikasnost.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Control of pepper and tomato bacterial spot
T1  - Zaštita paprike i paradajza od prouzrokovača bakteriozne pegavosti lista i krastavosti plodova
EP  - 609
IS  - 6
SP  - 596
VL  - 45
ER  - 
@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato caused by Xanthomonas species, is one of the widespread and economically most important diseases of pepper and tomato. Due to the lack of resistant pepper and tomato genotypes, the emergence of new races of bacteria, and strains resistant to copper compounds, questionable seed quality and limited control practices, thesebacteria are limiting pepper production in Serbia. On tomato plants, under favourable weather conditions for disease development, these bacteria can cause major damage reflected in overall yield as well as in fruit quality. The protection of pepper and tomato, based on chemical methods such as use of copper-based compounds in combination with ethylene-bis-dithiocarbamates, do not provide a satisfactory disease control. Use of antibiotics in plant protection is not permitted in the EU as well as in Serbia. However, results of many authors show that satisfactory control of bacterial spot can be achieved by integrating positive effect of chemical treatments (copper compounds, systemic resistance activators) and various biological methods (antagonists, bacteriophages). However, nature of biological agents, as well as the specific mechanism of action of the systemic resistance activators, require careful optimization of the time and number of treatments in order to achieve maximum efficiency., Bakteriozna pegavost lista i krastavost plodova prouzrokovana Xanthomonas vrstama, je jedna od najrasprostranjenijih i ekonomski najznačajnijih bolesti paprike i paradajza u svetu. Usled nedostatka otpornih genotipova paprike i paradajza, pojave novih rasa bakterije, sojeva rezistentnih prema jedinjenjima bakra, sumnjivog kvaliteta semena i ograničenih mera kontrole, ova bakterioza predstavlja ograničavajući faktor proizvodnje paprike u Srbiji. Kada vremenski uslovi pogoduju razvoju bolesti na biljkama paradajza, ova bakterioza može prouzrokovati velike gubitke usled smanjenja ukupnog prinosa i kvaliteta plodova koji nisu pogodni za industrijsku preradu. U zaštiti paprike i paradajza kod nas dominiraju hemijske mere borbe, odnosno korišćenje preparata na bazi jedinjenja bakra u kombinaciji sa etilenbis-ditiokarbamatima koji ne obezbeđuju zadovoljavajući efekat zaštite. Antibiotici se ne mogu primenjivati usled zakonskih ograničenja u Evropskoj uniji. Integracijom pozitivnog efekta hemijskih metoda (bakarni preparati, aktivatori sistemične otpornosti) i različitih bioloških metoda (antagonostički sojevi bakterija, bakteriofagi) može se postići zadovoljavajući efekat zaštite. Priroda bioloških agenasa, kao i specifičan mehanizam dejstva aktivatora sistemične otpornosti, zahtevaju pažljivu optimizaciju vremena i broja tretmana kako bi se postigla maksimalna efikasnost.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Control of pepper and tomato bacterial spot, Zaštita paprike i paradajza od prouzrokovača bakteriozne pegavosti lista i krastavosti plodova",
pages = "609-596",
number = "6",
volume = "45"
}
Šević, M., Gašić, K., Ignjatov, M.,& Obradović, A.. (2017). Control of pepper and tomato bacterial spot. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 596-609.
Šević M, Gašić K, Ignjatov M, Obradović A. Control of pepper and tomato bacterial spot. in Biljni lekar. 2017;45(6):596-609..
Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Obradović, Aleksa, "Control of pepper and tomato bacterial spot" in Biljni lekar, 45, no. 6 (2017):596-609.

Distribution and significance of causal agents of bacterial spot of pepper and tomato

Ignjatov, Maja; Gašić, Katarina; Šević, Milan; Obradović, Aleksa

(Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2017)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/504
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
ER  - 
@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45"
}
Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.
Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596..
Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596.

First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia

Ignjatov, Maja; Milosević, D.; Nikolić, Zorica; Gvozdanović-Varga, Jelica; Tatić, M.; Popović, Tatjana; Ivanović, Žarko

(Amer Phytopathological Soc, St Paul, 2017)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, D.
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tatić, M.
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/506
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia
EP  - 382
IS  - 2
SP  - 382
VL  - 101
DO  - 10.1094/PDIS-09-16-1333-PDN
ER  - 
@article{
author = "Ignjatov, Maja and Milosević, D. and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tatić, M. and Popović, Tatjana and Ivanović, Žarko",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia",
pages = "382-382",
number = "2",
volume = "101",
doi = "10.1094/PDIS-09-16-1333-PDN"
}
Ignjatov, M., Milosević, D., Nikolić, Z., Gvozdanović-Varga, J., Tatić, M., Popović, T.,& Ivanović, Ž.. (2017). First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(2), 382-382.
https://doi.org/10.1094/PDIS-09-16-1333-PDN
Ignjatov M, Milosević D, Nikolić Z, Gvozdanović-Varga J, Tatić M, Popović T, Ivanović Ž. First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia. in Plant Disease. 2017;101(2):382-382.
doi:10.1094/PDIS-09-16-1333-PDN .
Ignjatov, Maja, Milosević, D., Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tatić, M., Popović, Tatjana, Ivanović, Žarko, "First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia" in Plant Disease, 101, no. 2 (2017):382-382,
https://doi.org/10.1094/PDIS-09-16-1333-PDN . .
4
3
3

First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia

Blagojević, Jovana; Ivanović, Žarko; Popović, Tatjana; Ignjatov, Maja; Vukojević, Jelena

(Amer Phytopathological Soc, St Paul, 2017)

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Vukojević, Jelena
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/493
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia
EP  - 385
IS  - 2
SP  - 384
VL  - 101
DO  - 10.1094/PDIS-08-16-1106-PDN
ER  - 
@article{
author = "Blagojević, Jovana and Ivanović, Žarko and Popović, Tatjana and Ignjatov, Maja and Vukojević, Jelena",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia",
pages = "385-384",
number = "2",
volume = "101",
doi = "10.1094/PDIS-08-16-1106-PDN"
}
Blagojević, J., Ivanović, Ž., Popović, T., Ignjatov, M.,& Vukojević, J.. (2017). First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(2), 384-385.
https://doi.org/10.1094/PDIS-08-16-1106-PDN
Blagojević J, Ivanović Ž, Popović T, Ignjatov M, Vukojević J. First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease. 2017;101(2):384-385.
doi:10.1094/PDIS-08-16-1106-PDN .
Blagojević, Jovana, Ivanović, Žarko, Popović, Tatjana, Ignjatov, Maja, Vukojević, Jelena, "First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia" in Plant Disease, 101, no. 2 (2017):384-385,
https://doi.org/10.1094/PDIS-08-16-1106-PDN . .
1
2
1

First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia

Ignjatov, Maja; Bjelić, D.; Nikolić, Zorica; Milosević, D.; Gvozdanović-Varga, Jelica; Marinković, Jelena; Ivanović, Žarko

(Amer Phytopathological Soc, St Paul, 2017)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, D.
AU  - Nikolić, Zorica
AU  - Milosević, D.
AU  - Gvozdanović-Varga, Jelica
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/491
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia
EP  - 1048
IS  - 6
SP  - 1047
VL  - 101
DO  - 10.1094/PDIS-11-16-1625-PDN
ER  - 
@article{
author = "Ignjatov, Maja and Bjelić, D. and Nikolić, Zorica and Milosević, D. and Gvozdanović-Varga, Jelica and Marinković, Jelena and Ivanović, Žarko",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia",
pages = "1048-1047",
number = "6",
volume = "101",
doi = "10.1094/PDIS-11-16-1625-PDN"
}
Ignjatov, M., Bjelić, D., Nikolić, Z., Milosević, D., Gvozdanović-Varga, J., Marinković, J.,& Ivanović, Ž.. (2017). First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(6), 1047-1048.
https://doi.org/10.1094/PDIS-11-16-1625-PDN
Ignjatov M, Bjelić D, Nikolić Z, Milosević D, Gvozdanović-Varga J, Marinković J, Ivanović Ž. First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia. in Plant Disease. 2017;101(6):1047-1048.
doi:10.1094/PDIS-11-16-1625-PDN .
Ignjatov, Maja, Bjelić, D., Nikolić, Zorica, Milosević, D., Gvozdanović-Varga, Jelica, Marinković, Jelena, Ivanović, Žarko, "First Report of Fusarium acuminatum Causing Garlic Bulb Rot in Serbia" in Plant Disease, 101, no. 6 (2017):1047-1048,
https://doi.org/10.1094/PDIS-11-16-1625-PDN . .
4
3
5

First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia

Ivanović, Žarko; Blagojević, Jovana; Popović, Tatjana; Ignjatov, Maja

(Amer Phytopathological Soc, St Paul, 2017)

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/480
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia
EP  - 1678
IS  - 9
SP  - 1677
VL  - 101
DO  - 10.1094/PDIS-09-16-1327-PDN
ER  - 
@article{
author = "Ivanović, Žarko and Blagojević, Jovana and Popović, Tatjana and Ignjatov, Maja",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia",
pages = "1678-1677",
number = "9",
volume = "101",
doi = "10.1094/PDIS-09-16-1327-PDN"
}
Ivanović, Ž., Blagojević, J., Popović, T.,& Ignjatov, M.. (2017). First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(9), 1677-1678.
https://doi.org/10.1094/PDIS-09-16-1327-PDN
Ivanović Ž, Blagojević J, Popović T, Ignjatov M. First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia. in Plant Disease. 2017;101(9):1677-1678.
doi:10.1094/PDIS-09-16-1327-PDN .
Ivanović, Žarko, Blagojević, Jovana, Popović, Tatjana, Ignjatov, Maja, "First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia" in Plant Disease, 101, no. 9 (2017):1677-1678,
https://doi.org/10.1094/PDIS-09-16-1327-PDN . .
1
2
1

Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia

Ignjatov, Maja; Popović, Tatjana; Milošević, Dragana; Vasić, Mirjana; Nikolić, Zorica; Tamindžić, Gordana; Ivanović, Žarko

(Institute of field and vegetable crops, Novi Sad, 2016)

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/460
AB  - During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
AB  - Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
T1  - Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji
EP  - 45
IS  - 2
SP  - 42
VL  - 53
DO  - 10.5937/ratpov53-9984
ER  - 
@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Vasić, Mirjana and Nikolić, Zorica and Tamindžić, Gordana and Ivanović, Žarko",
year = "2016",
abstract = "During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot., Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia, Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji",
pages = "45-42",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9984"
}
Ignjatov, M., Popović, T., Milošević, D., Vasić, M., Nikolić, Z., Tamindžić, G.,& Ivanović, Ž.. (2016). Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 53(2), 42-45.
https://doi.org/10.5937/ratpov53-9984
Ignjatov M, Popović T, Milošević D, Vasić M, Nikolić Z, Tamindžić G, Ivanović Ž. Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo. 2016;53(2):42-45.
doi:10.5937/ratpov53-9984 .
Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Vasić, Mirjana, Nikolić, Zorica, Tamindžić, Gordana, Ivanović, Žarko, "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia" in Ratarstvo i povrtarstvo, 53, no. 2 (2016):42-45,
https://doi.org/10.5937/ratpov53-9984 . .
2

Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment

Popović, Tatjana; Ivanović, Žarko; Janjatović, Sanja; Ignjatov, Maja; Milovanović, Predrag

(Institute of field and vegetable crops, Novi Sad, 2016)

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Janjatović, Sanja
AU  - Ignjatov, Maja
AU  - Milovanović, Predrag
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/456
AB  - Brown rot or bacterial wilt caused by bacterium Ralstonia solanacearum is the main limiting factor in potato production. Quarantine measures are necessary to avoid spread of disease to disease-free areas. R. solanacearum has been shown to contaminate watercourses from which crop irrigation is then prohibited causing further potential losses in yield and quality. The bacteria also spread via surfaces that diseased seed potatoes come into contact with. This study showed bactericidal activity of chlorine dioxide (CIO2) on R. solanacearum for disinfection of water, surface and equipment. The results showed that CIO2 solution at concentration of 2 ppm at 30 minutes of exposure time had bactericidal effect for disinfection of water. For surface and equipment disinfection, concentration of 50 ppm showed total efficacy at 30 min and 5 sec exposure time, respectively. Results suggest that use of CIO2 as a disinfectant has a potential for control of brown rot pathogen in water, storage and equipment.
AB  - Mrka trulež ili bakteriozna uvelost krompira prozrokovana bakterijom Ralstonia solanacearum ograničavajući je faktor uspešne proizvodnje krompira. Sprovođenje karantinskih mera zaštite je neophodno kako bi se izbeglo širenje bakterije u regione u kojima bolest nije prisutna. S obzirom da R. solanacearum može kontaminirati vodene tokove i izvore koji služe za navodnjavanje useva, zabrana korišćenja dovodi do dodatnih potencijalnih gubitaka u prinosu i kvalitetu krompira. Bakterija takođe kontaminira površine sa kojima zaraženi semenski krompir dolazi u dodir. U ovom radu prikazana je baktericidna aktivnost hlor-dioksida (CIO2) na bakteriju R. solanacearum u cilju dezinfekcije vode, površine i opreme. Dobijeni rezultati pokazuju da CIO2 ispoljava baktericidni efekat za dezinfekciju vode u koncentraciji od 2 ppm nakon 30 minuta ekspozicije, za dezinfekciju površine u koncentraciji 50 ppm nakon 30 minuta ekspozicije i za dezinfekciju opreme u koncentraciji 50 ppm nakon 5 sekundi ekspozicije. Rezultati dobijeni u ovom radu ukazuju da CIO2 poseduje potencijal kao dezinficijens za kontrolu prouzrokovača mrke truleži krompira u vodi, skladištu i opremi.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment
T1  - Hlor-dioksid kao dezinficijens za kontrolu Ralstonia solanacearum u vodi, skladištu i opremi
EP  - 84
IS  - 2
SP  - 81
VL  - 53
DO  - 10.5937/ratpov53-9982
ER  - 
@article{
author = "Popović, Tatjana and Ivanović, Žarko and Janjatović, Sanja and Ignjatov, Maja and Milovanović, Predrag",
year = "2016",
abstract = "Brown rot or bacterial wilt caused by bacterium Ralstonia solanacearum is the main limiting factor in potato production. Quarantine measures are necessary to avoid spread of disease to disease-free areas. R. solanacearum has been shown to contaminate watercourses from which crop irrigation is then prohibited causing further potential losses in yield and quality. The bacteria also spread via surfaces that diseased seed potatoes come into contact with. This study showed bactericidal activity of chlorine dioxide (CIO2) on R. solanacearum for disinfection of water, surface and equipment. The results showed that CIO2 solution at concentration of 2 ppm at 30 minutes of exposure time had bactericidal effect for disinfection of water. For surface and equipment disinfection, concentration of 50 ppm showed total efficacy at 30 min and 5 sec exposure time, respectively. Results suggest that use of CIO2 as a disinfectant has a potential for control of brown rot pathogen in water, storage and equipment., Mrka trulež ili bakteriozna uvelost krompira prozrokovana bakterijom Ralstonia solanacearum ograničavajući je faktor uspešne proizvodnje krompira. Sprovođenje karantinskih mera zaštite je neophodno kako bi se izbeglo širenje bakterije u regione u kojima bolest nije prisutna. S obzirom da R. solanacearum može kontaminirati vodene tokove i izvore koji služe za navodnjavanje useva, zabrana korišćenja dovodi do dodatnih potencijalnih gubitaka u prinosu i kvalitetu krompira. Bakterija takođe kontaminira površine sa kojima zaraženi semenski krompir dolazi u dodir. U ovom radu prikazana je baktericidna aktivnost hlor-dioksida (CIO2) na bakteriju R. solanacearum u cilju dezinfekcije vode, površine i opreme. Dobijeni rezultati pokazuju da CIO2 ispoljava baktericidni efekat za dezinfekciju vode u koncentraciji od 2 ppm nakon 30 minuta ekspozicije, za dezinfekciju površine u koncentraciji 50 ppm nakon 30 minuta ekspozicije i za dezinfekciju opreme u koncentraciji 50 ppm nakon 5 sekundi ekspozicije. Rezultati dobijeni u ovom radu ukazuju da CIO2 poseduje potencijal kao dezinficijens za kontrolu prouzrokovača mrke truleži krompira u vodi, skladištu i opremi.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment, Hlor-dioksid kao dezinficijens za kontrolu Ralstonia solanacearum u vodi, skladištu i opremi",
pages = "84-81",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9982"
}
Popović, T., Ivanović, Ž., Janjatović, S., Ignjatov, M.,& Milovanović, P.. (2016). Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 53(2), 81-84.
https://doi.org/10.5937/ratpov53-9982
Popović T, Ivanović Ž, Janjatović S, Ignjatov M, Milovanović P. Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment. in Ratarstvo i povrtarstvo. 2016;53(2):81-84.
doi:10.5937/ratpov53-9982 .
Popović, Tatjana, Ivanović, Žarko, Janjatović, Sanja, Ignjatov, Maja, Milovanović, Predrag, "Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment" in Ratarstvo i povrtarstvo, 53, no. 2 (2016):81-84,
https://doi.org/10.5937/ratpov53-9982 . .