TY  - JOUR
AU  - Pavlović, Danijela
AU  - Vrbničanin, Sava
AU  - Reinhardt, Charlie
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/274
AB  - Glyphosate may cause injury to non-target plants. The first detectable symptom after glyphosate treatment is the growth inhibition, followed by noticeable yellowing (chlorosis) of the treated tissue. Five to ten days after the treatment, the chlorosis turns into necrosis and the plants begin to die. Greenhouse research was conducted in 2007 to investigate the response of glyphosate resistant (GR) soybeans PAN 520 line and non-glyphosate resistant EGRET line of soybeans CO glyphosate trimesium sulphosate and to evaluate soybeans injury to help in weed resistance detection. The methods used to detect changes were dose response test, HPLC measurement based on glyphosate induced accumulation of shikimate, and morpho-anatomical changes (light and electron microscopy). Damaged chloroplasts are a clear indication of a glyphosate injury. If the injury rating is related to increased shikimate levels, there is greater certainty that differences among biotypes are due to glyphosate tolerance.
PB  - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - Crop Response to Glyphosate Trimesium Sulphosate
EP  - 589
IS  - 2
SP  - 582
VL  - 41
ER  - 

@article{
author = "Pavlović, Danijela and Vrbničanin, Sava and Reinhardt, Charlie",
year = "2013",
abstract = "Glyphosate may cause injury to non-target plants. The first detectable symptom after glyphosate treatment is the growth inhibition, followed by noticeable yellowing (chlorosis) of the treated tissue. Five to ten days after the treatment, the chlorosis turns into necrosis and the plants begin to die. Greenhouse research was conducted in 2007 to investigate the response of glyphosate resistant (GR) soybeans PAN 520 line and non-glyphosate resistant EGRET line of soybeans CO glyphosate trimesium sulphosate and to evaluate soybeans injury to help in weed resistance detection. The methods used to detect changes were dose response test, HPLC measurement based on glyphosate induced accumulation of shikimate, and morpho-anatomical changes (light and electron microscopy). Damaged chloroplasts are a clear indication of a glyphosate injury. If the injury rating is related to increased shikimate levels, there is greater certainty that differences among biotypes are due to glyphosate tolerance.",
publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "Crop Response to Glyphosate Trimesium Sulphosate",
pages = "589-582",
number = "2",
volume = "41"
}

Pavlović, D., Vrbničanin, S.,& Reinhardt, C.. (2013). Crop Response to Glyphosate Trimesium Sulphosate. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 41(2), 582-589.

Pavlović D, Vrbničanin S, Reinhardt C. Crop Response to Glyphosate Trimesium Sulphosate. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2013;41(2):582-589..

Pavlović, Danijela, Vrbničanin, Sava, Reinhardt, Charlie, "Crop Response to Glyphosate Trimesium Sulphosate" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41, no. 2 (2013):582-589.

TY  - JOUR
AU  - Pavlović, Danijela
AU  - Reinhardt, Charlie
AU  - Božić, Dragana
AU  - Vrbničanin, Sava
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/282
AB  - Changes in leaf anatomy were examined in two S. African populations of Conyza canadensis of which one was presumed to be resistant (CCPR) and the other susceptible (CCS) to glyphosate. Glyphosate was applied to plants, which were grown from seed collected from these populations, at rates of 1, 2 and 4 kg a.e. ha(-1) of TOUCHDOWN [active substance: glyphosate trimesium salt, 500 g L-1] that are equivalent to 2, 4 and 8 L ha (-1) of the herbicide Touchdown. Leaf samples for the light microscope (LM) analysis were collected 3, 7 and 24 h after treatment (HAT). Changes in chlorophyll and shikimate content of leaf material were also examined. Changes in the palisade and pith tissue of leaves were not detected in the investigated populations at 3 and 7 HAT. However, at 24 HAT the different herbicide doses caused changes in leaf anatomy. These changes (injuries) were detected in the CCS at all tested doses, but in the CCPR population of C. canadensis the injuries were observed at only the two highest rates, 2 and 4 kg a.e. ha(-1). Chlorophyll and shikimate contents indicated significant differences between the treated and untreated plants of susceptible population only. Difference in glyphosate resistance between the CCS and CCPR populations was confirmed with an index of resistance of 1.58. This value of the index of resistance indicates that CCS population is 1.58 times more susceptible to glyphosate compared to CCPR population. (C) 2013 Friends Science Publishers
PB  - Friends Science Publ, Faisalabad
T2  - International Journal of Agriculture and Biology
T1  - Determination of Conyza canadensis Levels of Sensitivity to Glyphosate Trimesium Sulphosate
EP  - 1097
IS  - 6
SP  - 1091
VL  - 15
ER  - 

@article{
author = "Pavlović, Danijela and Reinhardt, Charlie and Božić, Dragana and Vrbničanin, Sava",
year = "2013",
abstract = "Changes in leaf anatomy were examined in two S. African populations of Conyza canadensis of which one was presumed to be resistant (CCPR) and the other susceptible (CCS) to glyphosate. Glyphosate was applied to plants, which were grown from seed collected from these populations, at rates of 1, 2 and 4 kg a.e. ha(-1) of TOUCHDOWN [active substance: glyphosate trimesium salt, 500 g L-1] that are equivalent to 2, 4 and 8 L ha (-1) of the herbicide Touchdown. Leaf samples for the light microscope (LM) analysis were collected 3, 7 and 24 h after treatment (HAT). Changes in chlorophyll and shikimate content of leaf material were also examined. Changes in the palisade and pith tissue of leaves were not detected in the investigated populations at 3 and 7 HAT. However, at 24 HAT the different herbicide doses caused changes in leaf anatomy. These changes (injuries) were detected in the CCS at all tested doses, but in the CCPR population of C. canadensis the injuries were observed at only the two highest rates, 2 and 4 kg a.e. ha(-1). Chlorophyll and shikimate contents indicated significant differences between the treated and untreated plants of susceptible population only. Difference in glyphosate resistance between the CCS and CCPR populations was confirmed with an index of resistance of 1.58. This value of the index of resistance indicates that CCS population is 1.58 times more susceptible to glyphosate compared to CCPR population. (C) 2013 Friends Science Publishers",
publisher = "Friends Science Publ, Faisalabad",
journal = "International Journal of Agriculture and Biology",
title = "Determination of Conyza canadensis Levels of Sensitivity to Glyphosate Trimesium Sulphosate",
pages = "1097-1091",
number = "6",
volume = "15"
}

Pavlović, D., Reinhardt, C., Božić, D.,& Vrbničanin, S.. (2013). Determination of Conyza canadensis Levels of Sensitivity to Glyphosate Trimesium Sulphosate. in International Journal of Agriculture and Biology
Friends Science Publ, Faisalabad., 15(6), 1091-1097.

Pavlović D, Reinhardt C, Božić D, Vrbničanin S. Determination of Conyza canadensis Levels of Sensitivity to Glyphosate Trimesium Sulphosate. in International Journal of Agriculture and Biology. 2013;15(6):1091-1097..

Pavlović, Danijela, Reinhardt, Charlie, Božić, Dragana, Vrbničanin, Sava, "Determination of Conyza canadensis Levels of Sensitivity to Glyphosate Trimesium Sulphosate" in International Journal of Agriculture and Biology, 15, no. 6 (2013):1091-1097.

TY  - JOUR
AU  - Pavlović, Danijela
AU  - Reinhardt, Charlie
AU  - Elezović, Igor
AU  - Vrbničanin, Sava
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/193
AB  - Glyphosate resistance was found in Lolium rigidum Gaudin (Rigid ryegrass, LOLRI) in South Africa. Suspected glyphosate-resistant L. rigidum populations were collected and grown under greenhouse conditions. The plants were sprayed with a range of doses of glyphosate 35 days after planting and shoot dry biomass was determined 17 days after herbicide treatment. Based on the dose-response experiment conducted in the greenhouse, one population of L. rigidum suspected to be resistant to glyphosate was approximately 5.3 fold more resistant than susceptible population. The other population was 2.8 fold more resistant than susceptible population. Difference between the two suspected resistant populations was 1.9 fold. All plants were treated with glyphosate (1000 g a.i. ha-1) and shikimic acid was extracted 2, 4 and 6 days after treatment. The plants of susceptible populations accumulated more shikimic acid than other two populations.
AB  - Rezistentnost na glifosat je potvrđena kod Lolium rigidum u Južnoj Africi. Semena rezistentne, očekivano glifosat-rezistentne i osetljive populacije L. rigidum su sakupljena u usevu pšenice sa različitih lokaliteta na području Južnoafričke Republike. Testiranje rezistentnosti na glifosat je izvedena u kontrolisanim uslovima staklare Univerziteta u Pretoriji (Južnoafrička Republika). Tridesetpet dana nakon setve (u plastičnim sudovima) biljke su tretirane suspenzijom od 125, 250, 500, 1000, 2000, 4000, 8000 i 12000 ml ha-1 glifosata, a suva masa je izmerena 17 dana nakon primene herbicida. Za fitovanje krive i utvrđivanje efektivne doze (dose-response-test) korišćena je jednačina ne-linearne regresione analize (R softver, drc paket). Na osnovu dobijenih rezultata utvrđena je 5,3 puta veća otpornost na glifosat kod rezistentne populacije L. rigidum (RLR) u poređenju sa osetljivom populacijom (OLR). Kod pretpostavljeno rezistentne populacije (PRP) konstatovana je 2,8 puta veća otpornost u odnosu na osetljivu populaciju. Razlika između pretpostavljeno rezistentne i rezistentne populacije je bila 1,9 puta. Za utvđivanje efekta glifosata na sadržaj šikiminske kiseline biljke su tretirane suspenzijom od 1000 g a.m. ha-1, a sadržaj šikiminske kiseline (HPLC metodom) je meren 2, 4 i 6 dana posle primene herbicida. Sadržaj šikiminske kiseline je bio veći kod osetljive populacije u poređenju sa druge dve testirane populacije L. rigidum. Dakle, potvrđena je rezistentnost RLR populacije, odnosno osetljivost OLR populacije, što obavezuje farmere da sprovode elemente antirezistentne strategije da bi usporili i sprečili ekspanziju rezistentnih populacija L. rigidum na području Južnoafričke Republike. Takođe, ta iskustva treba preneti i na druga područja u svetu da bi se sprečilo/usporilo širenje rezistentnosti korova na glifosat.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Identification of glyphosate resistance in Lolium rigidum Gaudin
T1  - Testiranje rezistentnosti Lolium rigidum Gaudin na glifosat
EP  - 399
IS  - 4
SP  - 393
VL  - 26
DO  - 10.2298/PIF1104393P
ER  - 

@article{
author = "Pavlović, Danijela and Reinhardt, Charlie and Elezović, Igor and Vrbničanin, Sava",
year = "2011",
abstract = "Glyphosate resistance was found in Lolium rigidum Gaudin (Rigid ryegrass, LOLRI) in South Africa. Suspected glyphosate-resistant L. rigidum populations were collected and grown under greenhouse conditions. The plants were sprayed with a range of doses of glyphosate 35 days after planting and shoot dry biomass was determined 17 days after herbicide treatment. Based on the dose-response experiment conducted in the greenhouse, one population of L. rigidum suspected to be resistant to glyphosate was approximately 5.3 fold more resistant than susceptible population. The other population was 2.8 fold more resistant than susceptible population. Difference between the two suspected resistant populations was 1.9 fold. All plants were treated with glyphosate (1000 g a.i. ha-1) and shikimic acid was extracted 2, 4 and 6 days after treatment. The plants of susceptible populations accumulated more shikimic acid than other two populations., Rezistentnost na glifosat je potvrđena kod Lolium rigidum u Južnoj Africi. Semena rezistentne, očekivano glifosat-rezistentne i osetljive populacije L. rigidum su sakupljena u usevu pšenice sa različitih lokaliteta na području Južnoafričke Republike. Testiranje rezistentnosti na glifosat je izvedena u kontrolisanim uslovima staklare Univerziteta u Pretoriji (Južnoafrička Republika). Tridesetpet dana nakon setve (u plastičnim sudovima) biljke su tretirane suspenzijom od 125, 250, 500, 1000, 2000, 4000, 8000 i 12000 ml ha-1 glifosata, a suva masa je izmerena 17 dana nakon primene herbicida. Za fitovanje krive i utvrđivanje efektivne doze (dose-response-test) korišćena je jednačina ne-linearne regresione analize (R softver, drc paket). Na osnovu dobijenih rezultata utvrđena je 5,3 puta veća otpornost na glifosat kod rezistentne populacije L. rigidum (RLR) u poređenju sa osetljivom populacijom (OLR). Kod pretpostavljeno rezistentne populacije (PRP) konstatovana je 2,8 puta veća otpornost u odnosu na osetljivu populaciju. Razlika između pretpostavljeno rezistentne i rezistentne populacije je bila 1,9 puta. Za utvđivanje efekta glifosata na sadržaj šikiminske kiseline biljke su tretirane suspenzijom od 1000 g a.m. ha-1, a sadržaj šikiminske kiseline (HPLC metodom) je meren 2, 4 i 6 dana posle primene herbicida. Sadržaj šikiminske kiseline je bio veći kod osetljive populacije u poređenju sa druge dve testirane populacije L. rigidum. Dakle, potvrđena je rezistentnost RLR populacije, odnosno osetljivost OLR populacije, što obavezuje farmere da sprovode elemente antirezistentne strategije da bi usporili i sprečili ekspanziju rezistentnih populacija L. rigidum na području Južnoafričke Republike. Takođe, ta iskustva treba preneti i na druga područja u svetu da bi se sprečilo/usporilo širenje rezistentnosti korova na glifosat.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Identification of glyphosate resistance in Lolium rigidum Gaudin, Testiranje rezistentnosti Lolium rigidum Gaudin na glifosat",
pages = "399-393",
number = "4",
volume = "26",
doi = "10.2298/PIF1104393P"
}

Pavlović, D., Reinhardt, C., Elezović, I.,& Vrbničanin, S.. (2011). Identification of glyphosate resistance in Lolium rigidum Gaudin. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 26(4), 393-399.
https://doi.org/10.2298/PIF1104393P

Pavlović D, Reinhardt C, Elezović I, Vrbničanin S. Identification of glyphosate resistance in Lolium rigidum Gaudin. in Pesticidi i fitomedicina. 2011;26(4):393-399.
doi:10.2298/PIF1104393P .

Pavlović, Danijela, Reinhardt, Charlie, Elezović, Igor, Vrbničanin, Sava, "Identification of glyphosate resistance in Lolium rigidum Gaudin" in Pesticidi i fitomedicina, 26, no. 4 (2011):393-399,
https://doi.org/10.2298/PIF1104393P . .

TY  - JOUR
AU  - Pavlović, Danijela
AU  - Vrbničanin, Sava
AU  - Reinhardt, Charlie
AU  - Fischer, Albert
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/78
AB  - Changes in leaf anatomy were examined in suspectedly resistant populations of C. canadensis and C. bonariensis after application of 2, 4 and 8 L ha-1 of the product Touch down (500 g a.i. glyphosate L-1). Samples for the analysis (LM, TEM) were collected 3, 7 and 24 h after herbicide application. Changes in chlorophyll and shikimic acid contents were also examined in the populations tested. Changes in the anatomy of leaves were not detected in the investigated populations 3 and 7 h after herbicide application. However, 24 h after application of different herbicide rates, differences were observed in leaf anatomy. Injuries were detected in C. bonariensis population after application of 2 L ha-1, and in C. canadensis population after treatment with the highest glyphosate rate (8 L ha-1). Changes were detected on cell walls, chloroplast lamellae and chloroplasts themselves. Based on chlorophyll and shikimic acid contents, significant statistical differences were detected between the treated and control plants.
AB  - Praćene su promene u anatomskoj građi listova pretpostavljeno rezistentih populacija C. canadensis i C. bonariensis nakon primene 2, 4 i 8 L ha-1 Touch down (500 g a.m. glifosat L-1). Za analizu anatomske građe listova (LM, TEM) materijal je uzorkovan nakon 3, 7 i 24 h posle primene herbicida. Takođe, praćene su promene u sadržaju hlorofila i šikiminske kiseline kod testiranih populacija. Promene u anatomskoj građi listova nisu konstatovane kod ispitivanih populacija nakon 3 i 7 časova posle primene herbicida. Međutim, 24 časa nakon primene različitih količina uočene su razlike u anatomskoj građi listova. Oštećenja su bila uočljiva kod pop. C. bonariensis nakon primene 2 L ha-1, a kod pop. C. canadensis tek nakon primene najveće količine glifosata (8 L ha-1). Promene su uočene na ćelijskom zidu, lamelama hloroplasta, kao i na samim hloroplastama. Na osnovu sadržaja hlorofila i šikiminske kiseline takođe su utvrđene statistički značajne razlike između tretiranih i kontrolnih biljaka.
PB  - Herbološko društvo Srbije, Beograd
T2  - Acta herbologica
T1  - Susceptibility of the species Conyza canadensis (L) cronquist and Conyza bonariensis (L) cronquist to glyphosate
T1  - Osetljivost vrsta Conyza canadensis (L) cronquist i Conyza bonariensis (L) cronquist na glifosat
EP  - 185
IS  - 2
SP  - 179
VL  - 17
ER  - 

@article{
author = "Pavlović, Danijela and Vrbničanin, Sava and Reinhardt, Charlie and Fischer, Albert",
year = "2008",
abstract = "Changes in leaf anatomy were examined in suspectedly resistant populations of C. canadensis and C. bonariensis after application of 2, 4 and 8 L ha-1 of the product Touch down (500 g a.i. glyphosate L-1). Samples for the analysis (LM, TEM) were collected 3, 7 and 24 h after herbicide application. Changes in chlorophyll and shikimic acid contents were also examined in the populations tested. Changes in the anatomy of leaves were not detected in the investigated populations 3 and 7 h after herbicide application. However, 24 h after application of different herbicide rates, differences were observed in leaf anatomy. Injuries were detected in C. bonariensis population after application of 2 L ha-1, and in C. canadensis population after treatment with the highest glyphosate rate (8 L ha-1). Changes were detected on cell walls, chloroplast lamellae and chloroplasts themselves. Based on chlorophyll and shikimic acid contents, significant statistical differences were detected between the treated and control plants., Praćene su promene u anatomskoj građi listova pretpostavljeno rezistentih populacija C. canadensis i C. bonariensis nakon primene 2, 4 i 8 L ha-1 Touch down (500 g a.m. glifosat L-1). Za analizu anatomske građe listova (LM, TEM) materijal je uzorkovan nakon 3, 7 i 24 h posle primene herbicida. Takođe, praćene su promene u sadržaju hlorofila i šikiminske kiseline kod testiranih populacija. Promene u anatomskoj građi listova nisu konstatovane kod ispitivanih populacija nakon 3 i 7 časova posle primene herbicida. Međutim, 24 časa nakon primene različitih količina uočene su razlike u anatomskoj građi listova. Oštećenja su bila uočljiva kod pop. C. bonariensis nakon primene 2 L ha-1, a kod pop. C. canadensis tek nakon primene najveće količine glifosata (8 L ha-1). Promene su uočene na ćelijskom zidu, lamelama hloroplasta, kao i na samim hloroplastama. Na osnovu sadržaja hlorofila i šikiminske kiseline takođe su utvrđene statistički značajne razlike između tretiranih i kontrolnih biljaka.",
publisher = "Herbološko društvo Srbije, Beograd",
journal = "Acta herbologica",
title = "Susceptibility of the species Conyza canadensis (L) cronquist and Conyza bonariensis (L) cronquist to glyphosate, Osetljivost vrsta Conyza canadensis (L) cronquist i Conyza bonariensis (L) cronquist na glifosat",
pages = "185-179",
number = "2",
volume = "17"
}

Pavlović, D., Vrbničanin, S., Reinhardt, C.,& Fischer, A.. (2008). Susceptibility of the species Conyza canadensis (L) cronquist and Conyza bonariensis (L) cronquist to glyphosate. in Acta herbologica
Herbološko društvo Srbije, Beograd., 17(2), 179-185.

Pavlović D, Vrbničanin S, Reinhardt C, Fischer A. Susceptibility of the species Conyza canadensis (L) cronquist and Conyza bonariensis (L) cronquist to glyphosate. in Acta herbologica. 2008;17(2):179-185..

Pavlović, Danijela, Vrbničanin, Sava, Reinhardt, Charlie, Fischer, Albert, "Susceptibility of the species Conyza canadensis (L) cronquist and Conyza bonariensis (L) cronquist to glyphosate" in Acta herbologica, 17, no. 2 (2008):179-185.