TY  - JOUR
AU  - Jošić, Dragana
AU  - Pivić, Radmila
AU  - Miladinović, Miroslav
AU  - Starović, Mira
AU  - Pavlović, Snežana
AU  - Đurić, Simonida
AU  - Jarak, Mirjana
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/206
AB  - Antibiotic production by plant-associated microorganisms represents an environmentally compatible method of disease control in agriculture. However, a vide application of bacterial strains needs careful selection and genetic characterization. In this investigation, selected Pseudomonas strains were characterized by rep-PCR methods using ERIC and (GTG)5 primers, and partial 16S rDNA sequence analysis. None of strains produced homoserine lactones (C4, C6, C8) as quorum sensing signal molecules. Very poor production of phenazines and no significant fungal inhibition was observed for PS4 and PS6 strains. High amount of phenazines were produced by Pseudomonas sp. strain PS2, which inhibited mycelial growth of 10 phytopatogenic fungi in percent of 25 (Verticillium sp.) to 65 (Fusarium equiseti). Genetic characterization of the Pseudomonas sp. PS2 and evaluation of phenazines production, as the main trait for growth inhibition of phytopathogenic fungi, will allow its application as a biosafe PGPR for field experiments of plant disease control.
AB  - Ekološki prihvatljiv metod kontrole bolesti bilja u poljoprivredi obuhvata primenu rizosfernih mikroorganizama koji produkuju antibiotike. Široka primena bakterija zahteva njihovu pažljivu selekciju i genetičku karakterizaciju. Ova istraživanja obuhvatila su karakterizaciju selektovanih Pseudomonas sojeva na osnovu rep-PCR metode korišćenjem ERIC i (GTG)5 prajmera, kao i parcijalne sekvence 16S rDNA. Nije detektovana produkcija acil-homoserin laktona (C4, C6, C8) kao QS molekula. Sojevi PS4 i PS6 su vrlo oskudno produkovali fenazine i nisu značajno inhibirali rast fitopatogenih gljiva. Soj Pseudomonas sp. PS2 je obilno produkovao 2-OH-PCA i PCA i inhibirao rast micelija 10 fitopatogenih gljiva od 25% (Verticillium sp.) do 65% (Fusarium equiseti). Genetička karakterizacija soja Pseudomonas sp. PS2 i detekcija produkcije fenazina kao glavnog inhibitora rasta fitopatogenih gljiva omogućiće primenu ovog soja kao bezbednog PGP agensa za biološku kontrolu u budućim ogledima u polju.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina
T1  - Antifungalna aktivnost i genetički diverzitet selektovanih Pseudomonas spp. iz rizosfere kukuruza u Vojvodini
EP  - 388
IS  - 2
SP  - 377
VL  - 44
DO  - 10.2298/GENSR1202377J
ER  - 

@article{
author = "Jošić, Dragana and Pivić, Radmila and Miladinović, Miroslav and Starović, Mira and Pavlović, Snežana and Đurić, Simonida and Jarak, Mirjana",
year = "2012",
abstract = "Antibiotic production by plant-associated microorganisms represents an environmentally compatible method of disease control in agriculture. However, a vide application of bacterial strains needs careful selection and genetic characterization. In this investigation, selected Pseudomonas strains were characterized by rep-PCR methods using ERIC and (GTG)5 primers, and partial 16S rDNA sequence analysis. None of strains produced homoserine lactones (C4, C6, C8) as quorum sensing signal molecules. Very poor production of phenazines and no significant fungal inhibition was observed for PS4 and PS6 strains. High amount of phenazines were produced by Pseudomonas sp. strain PS2, which inhibited mycelial growth of 10 phytopatogenic fungi in percent of 25 (Verticillium sp.) to 65 (Fusarium equiseti). Genetic characterization of the Pseudomonas sp. PS2 and evaluation of phenazines production, as the main trait for growth inhibition of phytopathogenic fungi, will allow its application as a biosafe PGPR for field experiments of plant disease control., Ekološki prihvatljiv metod kontrole bolesti bilja u poljoprivredi obuhvata primenu rizosfernih mikroorganizama koji produkuju antibiotike. Široka primena bakterija zahteva njihovu pažljivu selekciju i genetičku karakterizaciju. Ova istraživanja obuhvatila su karakterizaciju selektovanih Pseudomonas sojeva na osnovu rep-PCR metode korišćenjem ERIC i (GTG)5 prajmera, kao i parcijalne sekvence 16S rDNA. Nije detektovana produkcija acil-homoserin laktona (C4, C6, C8) kao QS molekula. Sojevi PS4 i PS6 su vrlo oskudno produkovali fenazine i nisu značajno inhibirali rast fitopatogenih gljiva. Soj Pseudomonas sp. PS2 je obilno produkovao 2-OH-PCA i PCA i inhibirao rast micelija 10 fitopatogenih gljiva od 25% (Verticillium sp.) do 65% (Fusarium equiseti). Genetička karakterizacija soja Pseudomonas sp. PS2 i detekcija produkcije fenazina kao glavnog inhibitora rasta fitopatogenih gljiva omogućiće primenu ovog soja kao bezbednog PGP agensa za biološku kontrolu u budućim ogledima u polju.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina, Antifungalna aktivnost i genetički diverzitet selektovanih Pseudomonas spp. iz rizosfere kukuruza u Vojvodini",
pages = "388-377",
number = "2",
volume = "44",
doi = "10.2298/GENSR1202377J"
}

Jošić, D., Pivić, R., Miladinović, M., Starović, M., Pavlović, S., Đurić, S.,& Jarak, M.. (2012). Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina. in Genetika-Belgrade
Serbian Genetics Society., 44(2), 377-388.
https://doi.org/10.2298/GENSR1202377J

Jošić D, Pivić R, Miladinović M, Starović M, Pavlović S, Đurić S, Jarak M. Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina. in Genetika-Belgrade. 2012;44(2):377-388.
doi:10.2298/GENSR1202377J .

Jošić, Dragana, Pivić, Radmila, Miladinović, Miroslav, Starović, Mira, Pavlović, Snežana, Đurić, Simonida, Jarak, Mirjana, "Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina" in Genetika-Belgrade, 44, no. 2 (2012):377-388,
https://doi.org/10.2298/GENSR1202377J . .

TY  - JOUR
AU  - Đurić, Simonida
AU  - Pavić, Aleksandar B
AU  - Jarak, Mirjana
AU  - Pavlović, Snežana
AU  - Starović, Mira
AU  - Pivić, Radmila
AU  - Jošić, Dragana
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/151
AB  - Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from medicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR
EP  - 6590
IS  - 5
SP  - 6580
VL  - 16
ER  - 

@article{
author = "Đurić, Simonida and Pavić, Aleksandar B and Jarak, Mirjana and Pavlović, Snežana and Starović, Mira and Pivić, Radmila and Jošić, Dragana",
year = "2011",
abstract = "Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from medicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR",
pages = "6590-6580",
number = "5",
volume = "16"
}

Đurić, S., Pavić, A. B., Jarak, M., Pavlović, S., Starović, M., Pivić, R.,& Jošić, D.. (2011). Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 16(5), 6580-6590.

Đurić S, Pavić AB, Jarak M, Pavlović S, Starović M, Pivić R, Jošić D. Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. in Romanian Biotechnological Letters. 2011;16(5):6580-6590..

Đurić, Simonida, Pavić, Aleksandar B, Jarak, Mirjana, Pavlović, Snežana, Starović, Mira, Pivić, Radmila, Jošić, Dragana, "Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR" in Romanian Biotechnological Letters, 16, no. 5 (2011):6580-6590.