TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Barać, Goran
AU  - Nikolić, Dušan
AU  - Stošić, Nemanja
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1245
AB  - In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The
causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening
light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1
medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA)
using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified
Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods
confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed
on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation,
respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor
development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical
features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall
has previously been documented solely in Japan, there is presently no data on its wider occurrence.
Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in
Serbia will contribute to a better understanding of it at the global level.
PB  - MDPI Basel
T2  - Agronomy
T1  - In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia
IS  - 4
SP  - 851
VL  - 14
DO  - 10.3390/agronomy14040851
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Barać, Goran and Nikolić, Dušan and Stošić, Nemanja and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The
causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening
light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1
medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA)
using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified
Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods
confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed
on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation,
respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor
development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical
features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall
has previously been documented solely in Japan, there is presently no data on its wider occurrence.
Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in
Serbia will contribute to a better understanding of it at the global level.",
publisher = "MDPI Basel",
journal = "Agronomy",
title = "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia",
number = "4",
pages = "851",
volume = "14",
doi = "10.3390/agronomy14040851"
}

Iličić, R., Jelušić, A., Barać, G., Nikolić, D., Stošić, N., Scortichini, M.,& Popović Milovanović, T.. (2024). In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy
MDPI Basel., 14(4), 851.
https://doi.org/10.3390/agronomy14040851

Iličić R, Jelušić A, Barać G, Nikolić D, Stošić N, Scortichini M, Popović Milovanović T. In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy. 2024;14(4):851.
doi:10.3390/agronomy14040851 .

Iličić, Renata, Jelušić, Aleksandra, Barać, Goran, Nikolić, Dušan, Stošić, Nemanja, Scortichini, Marco, Popović Milovanović, Tatjana, "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia" in Agronomy, 14, no. 4 (2024):851,
https://doi.org/10.3390/agronomy14040851 . .

TY  - JOUR
AU  - Tomić, Andrija
AU  - Trkulja, Vojislav
AU  - Matić, Slavica
AU  - Trkulja, Nenad
AU  - Iličić, Renata
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://enauka.gov.rs/handle/123456789/897621
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1233
AB  - Pyrenophora teres is a pathogen causing a net blotch disease in cultivated barley, which is present worldwide
and can thus significantly reduce barley yields. This fungus also infects wild barley and other plants of the Hordeum
genus, as well as barley grass, wheat, oats and plants from various genera, including Agropyron, Bromus, Elymus, Hordelymus and Stipa. Based on the symptoms it causes on the infected barley plants, the pathogen can be divided into two
forms: P. teres f. teres, which causes net-like symptoms, and P. teres f. maculata, which causes blotchy symptoms. Infected seeds, stubble and plant debris, and volunteer and weed plants represent primary sources of pathogen inoculum.
During the growing season, the pathogen enters a sexual stage, developing pseudothecia with asci and ascospores. This
is followed by an asexual stage, during which conidiophores with conidia are formed. The conidial (anamorphic) stage is
much more common, whereby conidia is a source of inoculum for secondary infection during the barley growing season.
The first symptoms appear at the end of winter and the beginning of spring, often during the tilling phase. The most
characteristic symptoms form on barley leaves. Frequently, symptoms of the net form can be mistaken for other diseases
occurring on barley, making molecular analysis essential for accurate detection of P. teres, its forms, mating types and
hybrids. Current net blotch control measures are based on the combined application of cultural, chemical and biological
control methods and the selection of resistant varieties.
PB  - Czech Academy of Agricultural Sciences
T2  - Plant Protection Science
T1  - Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production
EP  - 30
IS  - 1
SP  - 1
VL  - 60
DO  - 10.17221/122/2023-PPS
ER  - 

@article{
author = "Tomić, Andrija and Trkulja, Vojislav and Matić, Slavica and Trkulja, Nenad and Iličić, Renata and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Pyrenophora teres is a pathogen causing a net blotch disease in cultivated barley, which is present worldwide
and can thus significantly reduce barley yields. This fungus also infects wild barley and other plants of the Hordeum
genus, as well as barley grass, wheat, oats and plants from various genera, including Agropyron, Bromus, Elymus, Hordelymus and Stipa. Based on the symptoms it causes on the infected barley plants, the pathogen can be divided into two
forms: P. teres f. teres, which causes net-like symptoms, and P. teres f. maculata, which causes blotchy symptoms. Infected seeds, stubble and plant debris, and volunteer and weed plants represent primary sources of pathogen inoculum.
During the growing season, the pathogen enters a sexual stage, developing pseudothecia with asci and ascospores. This
is followed by an asexual stage, during which conidiophores with conidia are formed. The conidial (anamorphic) stage is
much more common, whereby conidia is a source of inoculum for secondary infection during the barley growing season.
The first symptoms appear at the end of winter and the beginning of spring, often during the tilling phase. The most
characteristic symptoms form on barley leaves. Frequently, symptoms of the net form can be mistaken for other diseases
occurring on barley, making molecular analysis essential for accurate detection of P. teres, its forms, mating types and
hybrids. Current net blotch control measures are based on the combined application of cultural, chemical and biological
control methods and the selection of resistant varieties.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Plant Protection Science",
title = "Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production",
pages = "30-1",
number = "1",
volume = "60",
doi = "10.17221/122/2023-PPS"
}

Tomić, A., Trkulja, V., Matić, S., Trkulja, N., Iličić, R., Scortichini, M.,& Popović Milovanović, T.. (2024). Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production. in Plant Protection Science
Czech Academy of Agricultural Sciences., 60(1), 1-30.
https://doi.org/10.17221/122/2023-PPS

Tomić A, Trkulja V, Matić S, Trkulja N, Iličić R, Scortichini M, Popović Milovanović T. Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production. in Plant Protection Science. 2024;60(1):1-30.
doi:10.17221/122/2023-PPS .

Tomić, Andrija, Trkulja, Vojislav, Matić, Slavica, Trkulja, Nenad, Iličić, Renata, Scortichini, Marco, Popović Milovanović, Tatjana, "Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production" in Plant Protection Science, 60, no. 1 (2024):1-30,
https://doi.org/10.17221/122/2023-PPS . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1138
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
IS  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8, 2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8).
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8).
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023),
https://doi.org/10.3390/microorganisms11082122 . .