Savić, Dušan

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Fungicide efficacy in peach rusty spot control in Serbia

Dolovac, Nenad; Miletić, Novica; Aleksić, Goran; Savić, Dušan; Živković, Svetlana; Trkulja, Nenad; Bulajić, Aleksandra

(Institute of Pesticides and Environmental Protection, 2010)

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Miletić, Novica
AU  - Aleksić, Goran
AU  - Savić, Dušan
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Bulajić, Aleksandra
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/120
AB  - Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O'Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur) for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the untreated control plots, high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial), flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively) while sulfur was the least efficient (82.33% and 80.30%, respectively). Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic information for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures.
AB  - Rđasta mrežavost plodova breskve odavno je poznato i ekonomski značajno oboljenje u mnogim delovima sveta. U Srbiji tokom proteklih godina ovo oboljenje nanosi značajne štete na poznim sortama breskve (Summerset, Suncrest, Fayette i O'Henry). Mada etiologija bolesti nije poznata, brojna istraživanja u mnogim delovima sveta pokušavaju da uspostave strategiju za kontrolu i preporuče odgovarajuće hemijske i druge metode zaštite breskve. U agroekološkim uslovima Srbije strategija primenjena u svetu, da suzbijanje prouzrokovača rđaste mrežavosti treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate. U pojedinim godinama zaraza plodova osetljivih sorti breskve dostiže 100%. U periodu od 2003. do 2004. godine, ispitivana je efikasnost tri fungicida (kresoksim-metil, fluzilazol i elementarni sumpor) u cilju iznalaženja mogućnosti uspešnije zaštite breskve od prouzrokovača rđaste mrežavosti plodova. Ogled je izveden na breskvi, sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Intenzitet zaraze rđaste mrežavosti plodova na osnovu procenta zahvaćene površine plodova, u kontrolnim parcelama kretao se od 33,5% u prvoj do 35,4% u drugoj godini ispitivanja. Tokom obe godine ispitivanja kresoksim-metil ispoljio je najveću efikasnost (90,25% u prvoj, odnosno 91,12% u drugoj godini ispitivanja), nižu fluzilazol (87,28%, odnosno 87,61%) i najnižu elementarni sumpor (82,33%, odnosno 80,30%). Određivanje fungicida sa najefikasnijim delovanjem prema prouzrokovaču rđaste mrežavosti plodova breskve u Srbiji predstavlja preduslov za dalja ispitivanja koja će uključiti pre svega optimizaciju rokova fungicidnih tretmana kao i određivanje drugih agrotehničkih mera kontrole.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Fungicide efficacy in peach rusty spot control in Serbia
T1  - Efikasnost fungicida za suzbijanje prouzrokovača rđaste mrežavosti plodova breskve u Srbiji
EP  - 249
IS  - 3
SP  - 241
VL  - 25
DO  - 10.2298/PIF1003241D
ER  - 
@article{
author = "Dolovac, Nenad and Miletić, Novica and Aleksić, Goran and Savić, Dušan and Živković, Svetlana and Trkulja, Nenad and Bulajić, Aleksandra",
year = "2010",
abstract = "Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O'Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur) for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the untreated control plots, high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial), flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively) while sulfur was the least efficient (82.33% and 80.30%, respectively). Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic information for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures., Rđasta mrežavost plodova breskve odavno je poznato i ekonomski značajno oboljenje u mnogim delovima sveta. U Srbiji tokom proteklih godina ovo oboljenje nanosi značajne štete na poznim sortama breskve (Summerset, Suncrest, Fayette i O'Henry). Mada etiologija bolesti nije poznata, brojna istraživanja u mnogim delovima sveta pokušavaju da uspostave strategiju za kontrolu i preporuče odgovarajuće hemijske i druge metode zaštite breskve. U agroekološkim uslovima Srbije strategija primenjena u svetu, da suzbijanje prouzrokovača rđaste mrežavosti treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate. U pojedinim godinama zaraza plodova osetljivih sorti breskve dostiže 100%. U periodu od 2003. do 2004. godine, ispitivana je efikasnost tri fungicida (kresoksim-metil, fluzilazol i elementarni sumpor) u cilju iznalaženja mogućnosti uspešnije zaštite breskve od prouzrokovača rđaste mrežavosti plodova. Ogled je izveden na breskvi, sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Intenzitet zaraze rđaste mrežavosti plodova na osnovu procenta zahvaćene površine plodova, u kontrolnim parcelama kretao se od 33,5% u prvoj do 35,4% u drugoj godini ispitivanja. Tokom obe godine ispitivanja kresoksim-metil ispoljio je najveću efikasnost (90,25% u prvoj, odnosno 91,12% u drugoj godini ispitivanja), nižu fluzilazol (87,28%, odnosno 87,61%) i najnižu elementarni sumpor (82,33%, odnosno 80,30%). Određivanje fungicida sa najefikasnijim delovanjem prema prouzrokovaču rđaste mrežavosti plodova breskve u Srbiji predstavlja preduslov za dalja ispitivanja koja će uključiti pre svega optimizaciju rokova fungicidnih tretmana kao i određivanje drugih agrotehničkih mera kontrole.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Fungicide efficacy in peach rusty spot control in Serbia, Efikasnost fungicida za suzbijanje prouzrokovača rđaste mrežavosti plodova breskve u Srbiji",
pages = "249-241",
number = "3",
volume = "25",
doi = "10.2298/PIF1003241D"
}
Dolovac, N., Miletić, N., Aleksić, G., Savić, D., Živković, S., Trkulja, N.,& Bulajić, A.. (2010). Fungicide efficacy in peach rusty spot control in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 25(3), 241-249.
https://doi.org/10.2298/PIF1003241D
Dolovac N, Miletić N, Aleksić G, Savić D, Živković S, Trkulja N, Bulajić A. Fungicide efficacy in peach rusty spot control in Serbia. in Pesticidi i fitomedicina. 2010;25(3):241-249.
doi:10.2298/PIF1003241D .
Dolovac, Nenad, Miletić, Novica, Aleksić, Goran, Savić, Dušan, Živković, Svetlana, Trkulja, Nenad, Bulajić, Aleksandra, "Fungicide efficacy in peach rusty spot control in Serbia" in Pesticidi i fitomedicina, 25, no. 3 (2010):241-249,
https://doi.org/10.2298/PIF1003241D . .
3

Efficacy of fungicides for control Monilinia laxa (Ader. i Ruhl.) in cheery orchard during the two-year study: 2008-2009

Trkulja, Nenad; Aleksić, Goran; Starović, Mira; Dolovac, Nenad; Ivanović, Žarko; Savić, Dušan; Gavrilović, Veljko

(Institute for Plant Protection and Environment, Belgrade, 2010)

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Dolovac, Nenad
AU  - Ivanović, Žarko
AU  - Savić, Dušan
AU  - Gavrilović, Veljko
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/129
AB  - Monilinia laxa (Ader. i Ruhl.) the causer agent of brown rot of stone fruit is widespread in all growing regions in Serbia. The disease severity depends of weather condition. Extended period of flowering and heavy precipitation are suitable for disease progress. The testing of fungicides for control M. laxa were carried out in two localities investigation during 2008 and 2009. The trials were set according to methods by OEEP/EPPO. The fungicide groups dicarboximides, benzimidazols and DMIs showed high efficiency in disease control.
AB  - Monilinia laxa (Ader. i Ruhl.), prouzrokovač sušenja cvetova i grančica koštičavog voća rasprostranjena je svuda gde se gaje ove voćne vrste. Ovo oboljenje se na našim prostorima redovno javlja na koštičavom voću, a intenzitet pojave zavisi od vremenskih uslova. Produžen period cvetanja i obilne količine padavina pogoduju razvoju bolesti, a štete u takvim godinama mogu biti značajne. Tokom 2008. i 2009. godine na dva lokaliteta ispitivana je efikasnost fungicida koji se koriste za suzbijanje M. laxa u našoj zemlji. Ogledi su postavljeni po OEEP/EPPO metodama. Dobijeni rezultati ukazuju na veoma visok stepen efikasnosti fungicida iz grupa dikarboksimida, benzimidazola i DMI (inhibitori demetilacije sterola) fungicida.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Efficacy of fungicides for control Monilinia laxa (Ader. i Ruhl.) in cheery orchard during the two-year study: 2008-2009
T1  - Efikasnost preparata za suzbijanje Monilinia laxa u zasadu višnje tokom dvogodišnjih ispitivanja - 2008-2009
EP  - 48
IS  - 1
SP  - 37
VL  - 61
ER  - 
@article{
author = "Trkulja, Nenad and Aleksić, Goran and Starović, Mira and Dolovac, Nenad and Ivanović, Žarko and Savić, Dušan and Gavrilović, Veljko",
year = "2010",
abstract = "Monilinia laxa (Ader. i Ruhl.) the causer agent of brown rot of stone fruit is widespread in all growing regions in Serbia. The disease severity depends of weather condition. Extended period of flowering and heavy precipitation are suitable for disease progress. The testing of fungicides for control M. laxa were carried out in two localities investigation during 2008 and 2009. The trials were set according to methods by OEEP/EPPO. The fungicide groups dicarboximides, benzimidazols and DMIs showed high efficiency in disease control., Monilinia laxa (Ader. i Ruhl.), prouzrokovač sušenja cvetova i grančica koštičavog voća rasprostranjena je svuda gde se gaje ove voćne vrste. Ovo oboljenje se na našim prostorima redovno javlja na koštičavom voću, a intenzitet pojave zavisi od vremenskih uslova. Produžen period cvetanja i obilne količine padavina pogoduju razvoju bolesti, a štete u takvim godinama mogu biti značajne. Tokom 2008. i 2009. godine na dva lokaliteta ispitivana je efikasnost fungicida koji se koriste za suzbijanje M. laxa u našoj zemlji. Ogledi su postavljeni po OEEP/EPPO metodama. Dobijeni rezultati ukazuju na veoma visok stepen efikasnosti fungicida iz grupa dikarboksimida, benzimidazola i DMI (inhibitori demetilacije sterola) fungicida.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Efficacy of fungicides for control Monilinia laxa (Ader. i Ruhl.) in cheery orchard during the two-year study: 2008-2009, Efikasnost preparata za suzbijanje Monilinia laxa u zasadu višnje tokom dvogodišnjih ispitivanja - 2008-2009",
pages = "48-37",
number = "1",
volume = "61"
}
Trkulja, N., Aleksić, G., Starović, M., Dolovac, N., Ivanović, Ž., Savić, D.,& Gavrilović, V.. (2010). Efficacy of fungicides for control Monilinia laxa (Ader. i Ruhl.) in cheery orchard during the two-year study: 2008-2009. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 61(1), 37-48.
Trkulja N, Aleksić G, Starović M, Dolovac N, Ivanović Ž, Savić D, Gavrilović V. Efficacy of fungicides for control Monilinia laxa (Ader. i Ruhl.) in cheery orchard during the two-year study: 2008-2009. in Zaštita bilja. 2010;61(1):37-48..
Trkulja, Nenad, Aleksić, Goran, Starović, Mira, Dolovac, Nenad, Ivanović, Žarko, Savić, Dušan, Gavrilović, Veljko, "Efficacy of fungicides for control Monilinia laxa (Ader. i Ruhl.) in cheery orchard during the two-year study: 2008-2009" in Zaštita bilja, 61, no. 1 (2010):37-48.

Fungi diseases of apricot

Aleksić, Goran; Popović, Tatjana; Milovanović, Predrag; Starović, Mira; Trkulja, Nenad; Savić, Dušan

(Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad, 2010)

TY  - JOUR
AU  - Aleksić, Goran
AU  - Popović, Tatjana
AU  - Milovanović, Predrag
AU  - Starović, Mira
AU  - Trkulja, Nenad
AU  - Savić, Dušan
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/147
AB  - This paper describes the most common diseases of apricot. Greatest economic importance of this fruit is a apoplexy, which is al ways current illness. Since the complex nature of this disease, the paper describes the phytopathogenic fungus that contribute to this phenomenon. These are: Monilinia laxa, M. fructigena, Cytospora cincta, Verticillium albo-atrum, V. dahliae and Eutypa lata. The paper mentioned and other phytopathogenic fungi that cause disease in apricot (Stigmina carpophila, Taphrina cerasi and Podosphaera tridactyla).
AB  - U radu su opisana najčešća oboljenja kajsije. Najveći ekonomski značaj za ovu voćnu vrstu predstavlja apopleksija, koja je posledica zajedničkog štetnog delovanja većeg broja fitopatogenih mikroorganizama. S obzirom da je ova bolest kompleksne prirode, u radu su opisane fitopatogene gljive koje doprinose ovoj pojavi. To su: Monilinia laxa, M. fructigena, Cytospora cincta, Verti cilli um albo-atrum, V. dahliae i Eutypa lata. U radu su pomenute i druge fitopatogene gljive koje prouzrokuju oboljenja na kajsiji (Stigmina carpophila, Taphrina cerasi i Podosphaera tridactyla).
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Fungi diseases of apricot
T1  - Mikoze kajsije
EP  - 316
IS  - 4-5
SP  - 310
VL  - 38
ER  - 
@article{
author = "Aleksić, Goran and Popović, Tatjana and Milovanović, Predrag and Starović, Mira and Trkulja, Nenad and Savić, Dušan",
year = "2010",
abstract = "This paper describes the most common diseases of apricot. Greatest economic importance of this fruit is a apoplexy, which is al ways current illness. Since the complex nature of this disease, the paper describes the phytopathogenic fungus that contribute to this phenomenon. These are: Monilinia laxa, M. fructigena, Cytospora cincta, Verticillium albo-atrum, V. dahliae and Eutypa lata. The paper mentioned and other phytopathogenic fungi that cause disease in apricot (Stigmina carpophila, Taphrina cerasi and Podosphaera tridactyla)., U radu su opisana najčešća oboljenja kajsije. Najveći ekonomski značaj za ovu voćnu vrstu predstavlja apopleksija, koja je posledica zajedničkog štetnog delovanja većeg broja fitopatogenih mikroorganizama. S obzirom da je ova bolest kompleksne prirode, u radu su opisane fitopatogene gljive koje doprinose ovoj pojavi. To su: Monilinia laxa, M. fructigena, Cytospora cincta, Verti cilli um albo-atrum, V. dahliae i Eutypa lata. U radu su pomenute i druge fitopatogene gljive koje prouzrokuju oboljenja na kajsiji (Stigmina carpophila, Taphrina cerasi i Podosphaera tridactyla).",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Fungi diseases of apricot, Mikoze kajsije",
pages = "316-310",
number = "4-5",
volume = "38"
}
Aleksić, G., Popović, T., Milovanović, P., Starović, M., Trkulja, N.,& Savić, D.. (2010). Fungi diseases of apricot. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 38(4-5), 310-316.
Aleksić G, Popović T, Milovanović P, Starović M, Trkulja N, Savić D. Fungi diseases of apricot. in Biljni lekar. 2010;38(4-5):310-316..
Aleksić, Goran, Popović, Tatjana, Milovanović, Predrag, Starović, Mira, Trkulja, Nenad, Savić, Dušan, "Fungi diseases of apricot" in Biljni lekar, 38, no. 4-5 (2010):310-316.

Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia

Gavrilović, Veljko; Ivanović, Žarko; Živković, Svetlana; Savić, Dušan

(Institute for Plant Protection and Environment, Belgrade, 2009)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Živković, Svetlana
AU  - Savić, Dušan
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/109
AB  - The test results of Pseudomonas syringae strains, isolated by a pear trees are as given. The symptoms of disesease, caused by this bacterium, appeared in two types: a blossom blast, a trunk necrosis and a branch followed by canker formation. All strains are Gram negative, fluorescent on a King medium B and oxydative (O/F test). The tested strains are HR positive, producing a levan, but don't oxydase, arginindehydrolase and pectinase (LOPAT +---+). Strains originated from a pear trees caused necrosis on an artificial inoculated pear, cherry and lemon fruits, as well as a syringae leaves and bean pods. The results of differential tests for P. syringae pv. syringae and P. syringae pv. morsprunorum (GATT) revealed that the tested strains hydrolise gelatin and esculin, but the negative results are recorded in tyrosinase production and tartrate utilization tests. The PCR analysis, by using a BOX primer, shows the high level of symilarity among the Serbian P. syringae strains, isolated from a pear fruit trees, but also within a slice differencies, compared with a check strain CFBP 1582. These results confirm a previous data about genetic diversity among P. syringae strains originated from a different areas all around the world. Based on the obtained results, it's concluded that the tested strains belong to P. syringae pv. syringae. Further characterization of P. syringae strains, isolated from pear in Serbia use the ERIC and REP PCR and it's still underway.
AB  - U radu su prikazani rezultati proučavanja fitopatogene bakterije Pseudomonas syringae, kao patogena kruške u Srbiji. Bolest se ispoljava u dva vida simptoma: palež cvasti i nekroza grana i debla mladih stabala kruške praćena obrazovanjem rak rana. Izolovani sojevi bakterije su Gramnegativni, fluorescentni, glukozu metabolišu isključivo u aerobnim uslovima (oksidativno). Stvaraju levan i prouzrokuju HR duvana ali ne stvaraju oksidazu, arginin dehidrolazu i pektinazu (LOPAT + - - - +). Prouzrokuju nekrozu inokulisanih plodova kruške, trešnje, limuna, listova jorgovana i mahuna boranije. Proučavani izolati hidrolizuju želatin i eskulin ali negativno reaguju pri testovima stvaranja tirozinaze i metabolizma tartarata (GATT). Na osnovu patogenih i biohemijskih odlika zaključeno je da proučavani izolati ispoljavaju izrazitu sličnost sa Psudomonas syringae pv. syringae, široko rasprostranjenim i ekonomski štetnim patogenom kruške. Primenom PCR analize korišćenjem BOX prajmera potvrđena je izrazita homogenost sojeva poreklom sa kruške, izolovanih u Srbiji, ali izvesne razlike u odnosu na kontrolne sojeve, što potvrđuje ranije dokazanu raznolikost sojeva ove bakterije u zavisnosti od lokaliteta i područja iz kog su izolovani. Na osnovu rezultata istraživanja dat je i predlog za uspostavljanje standardne procedure na bazi patogenih, biohemijskih i molekularnih metoda (primenom PCR) u cilju što brže i pouzdanije detekcije ove bakterije u biljnom materijalu, što je od velikog značaja pri proizvodnji sadnog materijala, proučavanja epidemiologije patogena i razrade mera njegovog suzbijanja.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia
T1  - Karakteristike izolata Pseudomonas syringae izolovanih sa kruške u Srbiji
EP  - 176
IS  - 3
SP  - 163
VL  - 60
ER  - 
@article{
author = "Gavrilović, Veljko and Ivanović, Žarko and Živković, Svetlana and Savić, Dušan",
year = "2009",
abstract = "The test results of Pseudomonas syringae strains, isolated by a pear trees are as given. The symptoms of disesease, caused by this bacterium, appeared in two types: a blossom blast, a trunk necrosis and a branch followed by canker formation. All strains are Gram negative, fluorescent on a King medium B and oxydative (O/F test). The tested strains are HR positive, producing a levan, but don't oxydase, arginindehydrolase and pectinase (LOPAT +---+). Strains originated from a pear trees caused necrosis on an artificial inoculated pear, cherry and lemon fruits, as well as a syringae leaves and bean pods. The results of differential tests for P. syringae pv. syringae and P. syringae pv. morsprunorum (GATT) revealed that the tested strains hydrolise gelatin and esculin, but the negative results are recorded in tyrosinase production and tartrate utilization tests. The PCR analysis, by using a BOX primer, shows the high level of symilarity among the Serbian P. syringae strains, isolated from a pear fruit trees, but also within a slice differencies, compared with a check strain CFBP 1582. These results confirm a previous data about genetic diversity among P. syringae strains originated from a different areas all around the world. Based on the obtained results, it's concluded that the tested strains belong to P. syringae pv. syringae. Further characterization of P. syringae strains, isolated from pear in Serbia use the ERIC and REP PCR and it's still underway., U radu su prikazani rezultati proučavanja fitopatogene bakterije Pseudomonas syringae, kao patogena kruške u Srbiji. Bolest se ispoljava u dva vida simptoma: palež cvasti i nekroza grana i debla mladih stabala kruške praćena obrazovanjem rak rana. Izolovani sojevi bakterije su Gramnegativni, fluorescentni, glukozu metabolišu isključivo u aerobnim uslovima (oksidativno). Stvaraju levan i prouzrokuju HR duvana ali ne stvaraju oksidazu, arginin dehidrolazu i pektinazu (LOPAT + - - - +). Prouzrokuju nekrozu inokulisanih plodova kruške, trešnje, limuna, listova jorgovana i mahuna boranije. Proučavani izolati hidrolizuju želatin i eskulin ali negativno reaguju pri testovima stvaranja tirozinaze i metabolizma tartarata (GATT). Na osnovu patogenih i biohemijskih odlika zaključeno je da proučavani izolati ispoljavaju izrazitu sličnost sa Psudomonas syringae pv. syringae, široko rasprostranjenim i ekonomski štetnim patogenom kruške. Primenom PCR analize korišćenjem BOX prajmera potvrđena je izrazita homogenost sojeva poreklom sa kruške, izolovanih u Srbiji, ali izvesne razlike u odnosu na kontrolne sojeve, što potvrđuje ranije dokazanu raznolikost sojeva ove bakterije u zavisnosti od lokaliteta i područja iz kog su izolovani. Na osnovu rezultata istraživanja dat je i predlog za uspostavljanje standardne procedure na bazi patogenih, biohemijskih i molekularnih metoda (primenom PCR) u cilju što brže i pouzdanije detekcije ove bakterije u biljnom materijalu, što je od velikog značaja pri proizvodnji sadnog materijala, proučavanja epidemiologije patogena i razrade mera njegovog suzbijanja.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia, Karakteristike izolata Pseudomonas syringae izolovanih sa kruške u Srbiji",
pages = "176-163",
number = "3",
volume = "60"
}
Gavrilović, V., Ivanović, Ž., Živković, S.,& Savić, D.. (2009). Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 60(3), 163-176.
Gavrilović V, Ivanović Ž, Živković S, Savić D. Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia. in Zaštita bilja. 2009;60(3):163-176..
Gavrilović, Veljko, Ivanović, Žarko, Živković, Svetlana, Savić, Dušan, "Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia" in Zaštita bilja, 60, no. 3 (2009):163-176.