Dolovac, Nenad

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  • Dolovac, Nenad (50)
Projects

Author's Bibliography

Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia

Ristić, Danijela; Vučurović, Ivan; Kuzmanović, Slobodan; Milosević, Drago; Gašić, Katarina; Dolovac, Nenad; Starović, Mira

(Serbian Genetics Society, 2016)

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Milosević, Drago
AU  - Gašić, Katarina
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/449
AB  - The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia
EP  - 496
IS  - 2
SP  - 487
VL  - 48
DO  - 10.2298/GENSR1602487R
ER  - 
@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Milosević, Drago and Gašić, Katarina and Dolovac, Nenad and Starović, Mira",
year = "2016",
abstract = "The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia",
pages = "496-487",
number = "2",
volume = "48",
doi = "10.2298/GENSR1602487R"
}
Ristić, D., Vučurović, I., Kuzmanović, S., Milosević, D., Gašić, K., Dolovac, N.,& Starović, M.. (2016). Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 48(2), 487-496.
https://doi.org/10.2298/GENSR1602487R
Ristić D, Vučurović I, Kuzmanović S, Milosević D, Gašić K, Dolovac N, Starović M. Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade. 2016;48(2):487-496.
doi:10.2298/GENSR1602487R .
Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Milosević, Drago, Gašić, Katarina, Dolovac, Nenad, Starović, Mira, "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia" in Genetika-Belgrade, 48, no. 2 (2016):487-496,
https://doi.org/10.2298/GENSR1602487R . .
2
1
2

Efikasnost različitih grupa fngicida u suzbijanju Venturia inaequalis u zasadu jabuke

Aleksic, Goran; Stevanović, Miloš; Kuzmanović, Slobodan; Starović, Mira; Dolovac, Nenad; Janković, Zoran

(Institut PKB Agroekonomik, 2015)

TY  - JOUR
AU  - Aleksic, Goran
AU  - Stevanović, Miloš
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Dolovac, Nenad
AU  - Janković, Zoran
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1029
PB  - Institut PKB Agroekonomik
T2  - Zbornik Instituta PKB agroekonomik
T1  - Efikasnost različitih grupa fngicida u suzbijanju Venturia inaequalis u zasadu jabuke
EP  - 53
IS  - 41
VL  - 5
VL  - 21
ER  - 
@article{
author = "Aleksic, Goran and Stevanović, Miloš and Kuzmanović, Slobodan and Starović, Mira and Dolovac, Nenad and Janković, Zoran",
year = "2015",
publisher = "Institut PKB Agroekonomik",
journal = "Zbornik Instituta PKB agroekonomik",
title = "Efikasnost različitih grupa fngicida u suzbijanju Venturia inaequalis u zasadu jabuke",
pages = "53",
number = "41",
volume = "5, 21"
}
Aleksic, G., Stevanović, M., Kuzmanović, S., Starović, M., Dolovac, N.,& Janković, Z.. (2015). Efikasnost različitih grupa fngicida u suzbijanju Venturia inaequalis u zasadu jabuke. in Zbornik Instituta PKB agroekonomik
Institut PKB Agroekonomik., 5(41).
Aleksic G, Stevanović M, Kuzmanović S, Starović M, Dolovac N, Janković Z. Efikasnost različitih grupa fngicida u suzbijanju Venturia inaequalis u zasadu jabuke. in Zbornik Instituta PKB agroekonomik. 2015;5(41):null-53..
Aleksic, Goran, Stevanović, Miloš, Kuzmanović, Slobodan, Starović, Mira, Dolovac, Nenad, Janković, Zoran, "Efikasnost različitih grupa fngicida u suzbijanju Venturia inaequalis u zasadu jabuke" in Zbornik Instituta PKB agroekonomik, 5, no. 41 (2015).

Efikasnost različitih grupa fungicida u suzbijanju Monilinia laxa (Aderhold & Ruhl.) Honez u zasadu višnje

Stevanović, Miloš; Aleksic, Goran; Dolovac, Nenad; Starović, Mira; Marković, S.; Janković, Z.

(Institut PKB Agroekonomik, 2015)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Aleksic, Goran
AU  - Dolovac, Nenad
AU  - Starović, Mira
AU  - Marković, S.
AU  - Janković, Z.
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1012
PB  - Institut PKB Agroekonomik
T2  - Zbornik Instituta PKB agroekonomik
T1  - Efikasnost različitih grupa fungicida u suzbijanju Monilinia laxa (Aderhold & Ruhl.) Honez u zasadu višnje
EP  - 32
IS  - 5
SP  - 25
VL  - 21
ER  - 
@article{
author = "Stevanović, Miloš and Aleksic, Goran and Dolovac, Nenad and Starović, Mira and Marković, S. and Janković, Z.",
year = "2015",
publisher = "Institut PKB Agroekonomik",
journal = "Zbornik Instituta PKB agroekonomik",
title = "Efikasnost različitih grupa fungicida u suzbijanju Monilinia laxa (Aderhold & Ruhl.) Honez u zasadu višnje",
pages = "32-25",
number = "5",
volume = "21"
}
Stevanović, M., Aleksic, G., Dolovac, N., Starović, M., Marković, S.,& Janković, Z.. (2015). Efikasnost različitih grupa fungicida u suzbijanju Monilinia laxa (Aderhold & Ruhl.) Honez u zasadu višnje. in Zbornik Instituta PKB agroekonomik
Institut PKB Agroekonomik., 21(5), 25-32.
Stevanović M, Aleksic G, Dolovac N, Starović M, Marković S, Janković Z. Efikasnost različitih grupa fungicida u suzbijanju Monilinia laxa (Aderhold & Ruhl.) Honez u zasadu višnje. in Zbornik Instituta PKB agroekonomik. 2015;21(5):25-32..
Stevanović, Miloš, Aleksic, Goran, Dolovac, Nenad, Starović, Mira, Marković, S., Janković, Z., "Efikasnost različitih grupa fungicida u suzbijanju Monilinia laxa (Aderhold & Ruhl.) Honez u zasadu višnje" in Zbornik Instituta PKB agroekonomik, 21, no. 5 (2015):25-32.

First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia

Blagojević, Jovana; Ivanović, Žarko; Oro, Violeta; Dolovac, Nenad; Popović Milovanović, Tatjana; Ignjatov, Maja; Vukojevic, Jelena

(American Phytopathological Society, 2015)

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Ivanović, Žarko
AU  - Oro, Violeta
AU  - Dolovac, Nenad
AU  - Popović Milovanović, Tatjana
AU  - Ignjatov, Maja
AU  - Vukojevic, Jelena
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/964
AB  - In July 2014, a foliar spot disease of horseradish (Armoracia rusticana) was observed in the Bačka region of Serbia (45°14′17″ N; 19°42′22″ E). The disease was observed in several small vegetable farms and disease incidence approached 20 to 40%. The symptoms first appeared on the abaxial side of older leaves as light brown concentric spots with dark margins and sometimes haloes. The spots varied in size between 0.5 and 0.9 cm. The necrotic spots gradually enlarged, tore, and dropped out. Under high humidity and temperature in 2014 in that region, development of numerous necrosis spots resulted in a rapid reduction of leaves. Small pieces taken from infected leaf tissue of diseased horseradish were treated with 1% sodium hypochlorite for 1 min, rinsed with sterile distillated water (SDW), and placed on V8 agar. Isolates were grown in pure culture at 24°C for 5 days in 12 h light/dark photoperiod. Morphological characteristics of the colony and sporulation were determined for six representative isolates. Fungal colonies were smooth, color varied from white, off white to light brown with concentric zones and intensive sporulation. Conidia were light brown, obclavate, produced singly or in short chains. Mature conidia were 16 to 38 µm wide and 73 to 158 µm long with a beak cell 79 to 120 µm long with 8 to 11 transverse septa and 0 to 4 longitudinal septa. Based on morphological characteristics, all isolates were identified as Alternaria brassicae (Berk.) Sacc (Simmons 2007). To confirm the pathogen’s identity, DNA was extracted from the fungal isolates and subjected to PCR; the internal transcribed spacer region of rDNA was amplified using primers ITS1 and ITS4 (White et al. 1990). The resulting amplicons were sequenced by Macrogen Inc. (Seoul, South Korea) and deposited in NCBI GenBank (Accession Nos. KP115599 to KP115604). BLAST analysis of revealed 100% homology with a sequence of A. brassicae strain ATCC 58169 deposited in GenBank (JX499028). Pathogenicity of six representative isolates was tested on leaves of 2-month-old horseradish seedlings by spraying with aqueous conidial suspensions (105 conidia/ml) prepared from cultures grown on V8 agar at 24°C for 5 days. Negative control plants were sprayed with SDW. Plants were covered with plastic bags and placed for 48 h in a greenhouse at 20 to 22°C. For all isolates within 10 days, inoculated plants developed brown lesions on leaves. Plants treated with SDW were symptomless. To fulfill Koch’s postulates, reisolations from all tested strains were done by streaking margins of necrotic leaf spot tissue onto V8 agar. Reisolated strains showed the same colony morphology as described above. Based on the pathogenicity test accompanied by completion of Koch’s postulates, sequence analysis, and morphological and cultural characteristics, the strains were identified as A. brassicae. According to available literature data, this is the first report of this pathogen as the causal agent of brown leaf spot of horseradish in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia
IS  - 5
SP  - 730
VL  - 99
DO  - 10.1094/PDIS-11-14-1181-PDN
ER  - 
@article{
author = "Blagojević, Jovana and Ivanović, Žarko and Oro, Violeta and Dolovac, Nenad and Popović Milovanović, Tatjana and Ignjatov, Maja and Vukojevic, Jelena",
year = "2015",
abstract = "In July 2014, a foliar spot disease of horseradish (Armoracia rusticana) was observed in the Bačka region of Serbia (45°14′17″ N; 19°42′22″ E). The disease was observed in several small vegetable farms and disease incidence approached 20 to 40%. The symptoms first appeared on the abaxial side of older leaves as light brown concentric spots with dark margins and sometimes haloes. The spots varied in size between 0.5 and 0.9 cm. The necrotic spots gradually enlarged, tore, and dropped out. Under high humidity and temperature in 2014 in that region, development of numerous necrosis spots resulted in a rapid reduction of leaves. Small pieces taken from infected leaf tissue of diseased horseradish were treated with 1% sodium hypochlorite for 1 min, rinsed with sterile distillated water (SDW), and placed on V8 agar. Isolates were grown in pure culture at 24°C for 5 days in 12 h light/dark photoperiod. Morphological characteristics of the colony and sporulation were determined for six representative isolates. Fungal colonies were smooth, color varied from white, off white to light brown with concentric zones and intensive sporulation. Conidia were light brown, obclavate, produced singly or in short chains. Mature conidia were 16 to 38 µm wide and 73 to 158 µm long with a beak cell 79 to 120 µm long with 8 to 11 transverse septa and 0 to 4 longitudinal septa. Based on morphological characteristics, all isolates were identified as Alternaria brassicae (Berk.) Sacc (Simmons 2007). To confirm the pathogen’s identity, DNA was extracted from the fungal isolates and subjected to PCR; the internal transcribed spacer region of rDNA was amplified using primers ITS1 and ITS4 (White et al. 1990). The resulting amplicons were sequenced by Macrogen Inc. (Seoul, South Korea) and deposited in NCBI GenBank (Accession Nos. KP115599 to KP115604). BLAST analysis of revealed 100% homology with a sequence of A. brassicae strain ATCC 58169 deposited in GenBank (JX499028). Pathogenicity of six representative isolates was tested on leaves of 2-month-old horseradish seedlings by spraying with aqueous conidial suspensions (105 conidia/ml) prepared from cultures grown on V8 agar at 24°C for 5 days. Negative control plants were sprayed with SDW. Plants were covered with plastic bags and placed for 48 h in a greenhouse at 20 to 22°C. For all isolates within 10 days, inoculated plants developed brown lesions on leaves. Plants treated with SDW were symptomless. To fulfill Koch’s postulates, reisolations from all tested strains were done by streaking margins of necrotic leaf spot tissue onto V8 agar. Reisolated strains showed the same colony morphology as described above. Based on the pathogenicity test accompanied by completion of Koch’s postulates, sequence analysis, and morphological and cultural characteristics, the strains were identified as A. brassicae. According to available literature data, this is the first report of this pathogen as the causal agent of brown leaf spot of horseradish in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia",
number = "5",
pages = "730",
volume = "99",
doi = "10.1094/PDIS-11-14-1181-PDN"
}
Blagojević, J., Ivanović, Ž., Oro, V., Dolovac, N., Popović Milovanović, T., Ignjatov, M.,& Vukojevic, J.. (2015). First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia. in Plant Disease
American Phytopathological Society., 99(5), 730.
https://doi.org/10.1094/PDIS-11-14-1181-PDN
Blagojević J, Ivanović Ž, Oro V, Dolovac N, Popović Milovanović T, Ignjatov M, Vukojevic J. First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia. in Plant Disease. 2015;99(5):730.
doi:10.1094/PDIS-11-14-1181-PDN .
Blagojević, Jovana, Ivanović, Žarko, Oro, Violeta, Dolovac, Nenad, Popović Milovanović, Tatjana, Ignjatov, Maja, Vukojevic, Jelena, "First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia" in Plant Disease, 99, no. 5 (2015):730,
https://doi.org/10.1094/PDIS-11-14-1181-PDN . .
5
3

First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia

Blagojević, Jovana; Ivanović, Žarko; Oro, Violeta; Dolovac, Nenad; Popović, Tatjana; Ignjatov, Maja; Vukojević, Jelena

(Amer Phytopathological Soc, St Paul, 2015)

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Ivanović, Žarko
AU  - Oro, Violeta
AU  - Dolovac, Nenad
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Vukojević, Jelena
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/417
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia
EP  - 730
IS  - 5
SP  - 730
VL  - 99
DO  - 10.1094/PDIS-11-14-1181-PDN
ER  - 
@article{
author = "Blagojević, Jovana and Ivanović, Žarko and Oro, Violeta and Dolovac, Nenad and Popović, Tatjana and Ignjatov, Maja and Vukojević, Jelena",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia",
pages = "730-730",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1181-PDN"
}
Blagojević, J., Ivanović, Ž., Oro, V., Dolovac, N., Popović, T., Ignjatov, M.,& Vukojević, J.. (2015). First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 730-730.
https://doi.org/10.1094/PDIS-11-14-1181-PDN
Blagojević J, Ivanović Ž, Oro V, Dolovac N, Popović T, Ignjatov M, Vukojević J. First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia. in Plant Disease. 2015;99(5):730-730.
doi:10.1094/PDIS-11-14-1181-PDN .
Blagojević, Jovana, Ivanović, Žarko, Oro, Violeta, Dolovac, Nenad, Popović, Tatjana, Ignjatov, Maja, Vukojević, Jelena, "First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia" in Plant Disease, 99, no. 5 (2015):730-730,
https://doi.org/10.1094/PDIS-11-14-1181-PDN . .
5
2
3

Copper-Citrate as a Possibility for Control of Some Phytopathogenic Bacteria

Popović Milovanović, Tatjana; Milićević, Zoran; Milovanović, Predrag; Dolovac, Nenad; Ivanović, Žarko

(Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India, 2014)

TY  - CHAP
AU  - Popović Milovanović, Tatjana
AU  - Milićević, Zoran
AU  - Milovanović, Predrag
AU  - Dolovac, Nenad
AU  - Ivanović, Žarko
PY  - 2014
UR  - https://www.atiner.gr/docs/2013AGR-INTRO.pdf
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1149
PB  - Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India
T2  - Technological Advancement for Vibrant Agriculture
T1  - Copper-Citrate as a Possibility for Control of Some Phytopathogenic Bacteria
EP  - 206
SP  - 201
ER  - 
@inbook{
author = "Popović Milovanović, Tatjana and Milićević, Zoran and Milovanović, Predrag and Dolovac, Nenad and Ivanović, Žarko",
year = "2014",
publisher = "Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India",
journal = "Technological Advancement for Vibrant Agriculture",
booktitle = "Copper-Citrate as a Possibility for Control of Some Phytopathogenic Bacteria",
pages = "206-201"
}
Popović Milovanović, T., Milićević, Z., Milovanović, P., Dolovac, N.,& Ivanović, Ž.. (2014). Copper-Citrate as a Possibility for Control of Some Phytopathogenic Bacteria. in Technological Advancement for Vibrant Agriculture
Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India., 201-206.
Popović Milovanović T, Milićević Z, Milovanović P, Dolovac N, Ivanović Ž. Copper-Citrate as a Possibility for Control of Some Phytopathogenic Bacteria. in Technological Advancement for Vibrant Agriculture. 2014;:201-206..
Popović Milovanović, Tatjana, Milićević, Zoran, Milovanović, Predrag, Dolovac, Nenad, Ivanović, Žarko, "Copper-Citrate as a Possibility for Control of Some Phytopathogenic Bacteria" in Technological Advancement for Vibrant Agriculture (2014):201-206.

Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia

Ristić, Danijela; Pavlović, Snežana; Trkulja, Nenad; Pfaf-Dolovac, Erika; Dolovac, Nenad; Starović, Mira

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/302
AB  - During 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide.
AB  - Tokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia
T1  - Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji
EP  - 116
IS  - 3
SP  - 111
VL  - 65
DO  - 10.5937/zasbilj1403111R
ER  - 
@article{
author = "Ristić, Danijela and Pavlović, Snežana and Trkulja, Nenad and Pfaf-Dolovac, Erika and Dolovac, Nenad and Starović, Mira",
year = "2014",
abstract = "During 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide., Tokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia, Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji",
pages = "116-111",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403111R"
}
Ristić, D., Pavlović, S., Trkulja, N., Pfaf-Dolovac, E., Dolovac, N.,& Starović, M.. (2014). Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 111-116.
https://doi.org/10.5937/zasbilj1403111R
Ristić D, Pavlović S, Trkulja N, Pfaf-Dolovac E, Dolovac N, Starović M. Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia. in Zaštita bilja. 2014;65(3):111-116.
doi:10.5937/zasbilj1403111R .
Ristić, Danijela, Pavlović, Snežana, Trkulja, Nenad, Pfaf-Dolovac, Erika, Dolovac, Nenad, Starović, Mira, "Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia" in Zaštita bilja, 65, no. 3 (2014):111-116,
https://doi.org/10.5937/zasbilj1403111R . .
1

Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia

Gašić, Katarina; Gavrilović, Veljko; Dolovac, Nenad; Trkulja, Nenad; Živković, Svetlana; Ristić, Danijela; Obradović, Aleksa

(Institute of Pesticides and Environmental Protection, 2014)

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/362
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 
@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}
Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G
Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .
Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .
11

Application of different Polyethylen glycole concentrations and evaluation of different methods for germination of alfalfa

Štrbanović, Ratibor; Stanisavljević, Rade; Đukanović, Lana; Poštić, Dobrivoj; Marković, Jordan; Đokić, Dragoslav; Dolovac, Nenad

(Nacionalno društvo za procesnu tehniku i eneregetiku u poljoprivredi, Novi Sad, 2014)

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Stanisavljević, Rade
AU  - Đukanović, Lana
AU  - Poštić, Dobrivoj
AU  - Marković, Jordan
AU  - Đokić, Dragoslav
AU  - Dolovac, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/353
AB  - This paper presents the germination three cultivars of alfalfa using different concentrations of PEG-6000 (Polyethylene glycol) and control. The aim of this study was to determine the tolerance of alfalfa under drought stress effects and the choice of optimal testing methods. Seed germination and establish the presence of hard seed alfalfa was done by the standard method without pretreatment and with pretreatment. Readout germination of alfalfa seeds was done after ten days, according to the Rules for testing seed quality of agricultural products. The concentration of PEG of 0.4 MPa has proven to be optimal for assessing the seed germination of alfalfa. The alfalfa cultivars have behaved differently towards different concentrations on PEG, indicating the possibility of correct selection of alfalfa cultivars for autumn sowing period (September) after seed harvest in August.
AB  - Lucerka je najvažnija višegodišnja i višeotkosna krmna biljka, koja daje visoke prinose i odličan kvalitet krme u različitim ekološkim uslovima. Gajena u kombinovanoj proizvodnji (seme/krma) tokom višegodišnjeg korišćenja veoma je varijabilna prema visini prinosa semena (CV = 29,4% do 47,5%) dok je prema visini prinosa krme sa nižom varijabilnšću (CV=17,1% do 25,7%), i najmanjom varijabilnošću za kvalitet semena (klijavost CV=5,4% do 6,4%). U radu je prikazana klijavost tri sorte lucerke primenom različitih koncentracija PEG-6000 (Polyethylene glycol) i kontrole. Cilj ovog rada je utvrđivanje tolerantnosti lucerke prema efektu stresa na sušu i izbor najoptimalnije metode ispitivanja. Ispitivanje klijavosti i utvrđivanje prisustva tvrdih semena lucerke rađeno je standardnom metodom bez predtretmana i sa predtretmanom. Očitavanje klijavosti semena lucerke urađeno je nakon deset dana, prema pravilniku za ispitivanje kvaliteta semena poljoprivrednog bilja. Koncentracija PEG-a od 0,4 Mpa pokazala se optimalnom za ocenu klijavosti semena lucerke. Sorte lucerke su se različito ponašale prema različitim koncentracijama na PEG, što ukazuje na mogućnost pravilnog izbora sorti lucerke za jesenji setveni rok (septembar) nakon žetve semena u avgustu mesecu. Sorte lucerketolerantnije na PEG u jesenjem setvenom roku, kada je i najčešće uspešnije za zasnivanje lucerišta. Ove sorte bi bile pogodne i za prema stresu na sušu.
PB  - Nacionalno društvo za procesnu tehniku i eneregetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Application of different Polyethylen glycole concentrations and evaluation of different methods for germination of alfalfa
T1  - Primena različitih koncentracija Polyethylene glycola i ocena različitih metoda na klijavost semena lucerke
EP  - 231
IS  - 5
SP  - 229
VL  - 18
ER  - 
@article{
author = "Štrbanović, Ratibor and Stanisavljević, Rade and Đukanović, Lana and Poštić, Dobrivoj and Marković, Jordan and Đokić, Dragoslav and Dolovac, Nenad",
year = "2014",
abstract = "This paper presents the germination three cultivars of alfalfa using different concentrations of PEG-6000 (Polyethylene glycol) and control. The aim of this study was to determine the tolerance of alfalfa under drought stress effects and the choice of optimal testing methods. Seed germination and establish the presence of hard seed alfalfa was done by the standard method without pretreatment and with pretreatment. Readout germination of alfalfa seeds was done after ten days, according to the Rules for testing seed quality of agricultural products. The concentration of PEG of 0.4 MPa has proven to be optimal for assessing the seed germination of alfalfa. The alfalfa cultivars have behaved differently towards different concentrations on PEG, indicating the possibility of correct selection of alfalfa cultivars for autumn sowing period (September) after seed harvest in August., Lucerka je najvažnija višegodišnja i višeotkosna krmna biljka, koja daje visoke prinose i odličan kvalitet krme u različitim ekološkim uslovima. Gajena u kombinovanoj proizvodnji (seme/krma) tokom višegodišnjeg korišćenja veoma je varijabilna prema visini prinosa semena (CV = 29,4% do 47,5%) dok je prema visini prinosa krme sa nižom varijabilnšću (CV=17,1% do 25,7%), i najmanjom varijabilnošću za kvalitet semena (klijavost CV=5,4% do 6,4%). U radu je prikazana klijavost tri sorte lucerke primenom različitih koncentracija PEG-6000 (Polyethylene glycol) i kontrole. Cilj ovog rada je utvrđivanje tolerantnosti lucerke prema efektu stresa na sušu i izbor najoptimalnije metode ispitivanja. Ispitivanje klijavosti i utvrđivanje prisustva tvrdih semena lucerke rađeno je standardnom metodom bez predtretmana i sa predtretmanom. Očitavanje klijavosti semena lucerke urađeno je nakon deset dana, prema pravilniku za ispitivanje kvaliteta semena poljoprivrednog bilja. Koncentracija PEG-a od 0,4 Mpa pokazala se optimalnom za ocenu klijavosti semena lucerke. Sorte lucerke su se različito ponašale prema različitim koncentracijama na PEG, što ukazuje na mogućnost pravilnog izbora sorti lucerke za jesenji setveni rok (septembar) nakon žetve semena u avgustu mesecu. Sorte lucerketolerantnije na PEG u jesenjem setvenom roku, kada je i najčešće uspešnije za zasnivanje lucerišta. Ove sorte bi bile pogodne i za prema stresu na sušu.",
publisher = "Nacionalno društvo za procesnu tehniku i eneregetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Application of different Polyethylen glycole concentrations and evaluation of different methods for germination of alfalfa, Primena različitih koncentracija Polyethylene glycola i ocena različitih metoda na klijavost semena lucerke",
pages = "231-229",
number = "5",
volume = "18"
}
Štrbanović, R., Stanisavljević, R., Đukanović, L., Poštić, D., Marković, J., Đokić, D.,& Dolovac, N.. (2014). Application of different Polyethylen glycole concentrations and evaluation of different methods for germination of alfalfa. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i eneregetiku u poljoprivredi, Novi Sad., 18(5), 229-231.
Štrbanović R, Stanisavljević R, Đukanović L, Poštić D, Marković J, Đokić D, Dolovac N. Application of different Polyethylen glycole concentrations and evaluation of different methods for germination of alfalfa. in Journal on Processing and Energy in Agriculture. 2014;18(5):229-231..
Štrbanović, Ratibor, Stanisavljević, Rade, Đukanović, Lana, Poštić, Dobrivoj, Marković, Jordan, Đokić, Dragoslav, Dolovac, Nenad, "Application of different Polyethylen glycole concentrations and evaluation of different methods for germination of alfalfa" in Journal on Processing and Energy in Agriculture, 18, no. 5 (2014):229-231.

Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion

Stevanović, Miloš; Dolovac, Nenad; Trkulja, Nenad; Milosavljević, Anja; Kuzmanović, Slobodan; Aleksić, Goran

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Kuzmanović, Slobodan
AU  - Aleksić, Goran
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/328
AB  - Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%).
AB  - Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion
T1  - Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije
EP  - 32
IS  - 1
SP  - 27
VL  - 65
DO  - 10.5937/zasbilj1401027S
ER  - 
@article{
author = "Stevanović, Miloš and Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Kuzmanović, Slobodan and Aleksić, Goran",
year = "2014",
abstract = "Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%)., Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion, Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije",
pages = "32-27",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401027S"
}
Stevanović, M., Dolovac, N., Trkulja, N., Milosavljević, A., Kuzmanović, S.,& Aleksić, G.. (2014). Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 27-32.
https://doi.org/10.5937/zasbilj1401027S
Stevanović M, Dolovac N, Trkulja N, Milosavljević A, Kuzmanović S, Aleksić G. Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja. 2014;65(1):27-32.
doi:10.5937/zasbilj1401027S .
Stevanović, Miloš, Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Kuzmanović, Slobodan, Aleksić, Goran, "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion" in Zaštita bilja, 65, no. 1 (2014):27-32,
https://doi.org/10.5937/zasbilj1401027S . .
1

The distribution of weeds in natural alfalfa seeds

Štrbanović, Ratibor; Poštić, Dobrivoj; Stanisavljević, Rade; Đukanović, Lana; Ivanović, Žarko; Vasić, Tanja; Dolovac, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Stanisavljević, Rade
AU  - Đukanović, Lana
AU  - Ivanović, Žarko
AU  - Vasić, Tanja
AU  - Dolovac, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/312
AB  - In this study the presence of seeds of various weed species in natural alfalfa seed of different cultivars from different localities. In all studied cultivars of alfalfa and in all localities was found 25 different weed species, of which 14 species were perennial weeds, while the other 11 were annual weed species. Since dangerous perennial weeds which proliferate by vegetative and generative identified by (Sorghum halepense and Cirsium arvense). The highest number of weed seeds had annual species Picris echioides L. cultivar K-22 at the site Ratari 446 seeds. Also a large number of seeds was determined and the perennial species Cichorium intybus L. in all the cultivars of alfalfa. The lowest number of seeds were found in the species Rumex sp. four seeds and only the cultivar NS-Mediana at the site Bačko Gradište I, as with species Conium maculatum seven seeds only in the cultivar K-28 at the site Banatsko Karađorđevo. Most weeds species present (nine) was found in alfalfa cultivars NS-Mediana at the site Bačko Gradište I, and while the lowest number of weeds species (two) was found in the cultivar Banjalučanka at the site Kozarska Dubica.
AB  - U ovom radu ispitivana je zastupljenost semena različitih korovskih vrsta u naturalnom semenu različitih sorata lucerke sa različitih lokaliteta. Kod svih ispitivanih sorti lucerke i na svim lokalitetima identifikovano je 25 različitih vrsta korova, od toga 14 vrsta su bili višegodišnji korovi, dok su ostalih 11 bile jednogodišnje korovske vrste. Od opasnih višegodišnjih korova koji se razmnožavaju generativnim i vegetativnim putem identifikovani su (Sorghum halepense i Cirsium arvense). Najveći broj semena korova imala je jednogodišnja vrsta Picris echioides L. kod sorte K-22 na lokalitetu Ratari (446). Takođe veliki broj semena utvrđen je i kod višegodišnje vrste Cichorium intybus L. i to kod svih ispitivanih sorata lucerke. Najmanji broj semena utvrđen je kod vrste Rumex sp. (četiri semena) i to jedino kod sorte NS-Mediana na lokalitetu Bačko Gradište I, kao i kod vrste Conium maculatum (sedam semena) samo kod sorte K-28 na lokalitetu Banatsko Karađorđevo. Najviše prisutnih korovskih vrsta (devet) utvrđeno je kod sorte lucerke NS-Mediana na lokalitetu Bačko Gradište I, dok je najmanji broj korovskih vrsta (dve) konstatovan kod sorte Banjalučanka na lokalitetu Kozarska Dubica.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - The distribution of weeds in natural alfalfa seeds
T1  - Zastupljenost korova u naturalnom semenu lucerke
EP  - 90
IS  - 2
SP  - 85
VL  - 65
DO  - 10.5937/zasbilj1402085S
ER  - 
@article{
author = "Štrbanović, Ratibor and Poštić, Dobrivoj and Stanisavljević, Rade and Đukanović, Lana and Ivanović, Žarko and Vasić, Tanja and Dolovac, Nenad",
year = "2014",
abstract = "In this study the presence of seeds of various weed species in natural alfalfa seed of different cultivars from different localities. In all studied cultivars of alfalfa and in all localities was found 25 different weed species, of which 14 species were perennial weeds, while the other 11 were annual weed species. Since dangerous perennial weeds which proliferate by vegetative and generative identified by (Sorghum halepense and Cirsium arvense). The highest number of weed seeds had annual species Picris echioides L. cultivar K-22 at the site Ratari 446 seeds. Also a large number of seeds was determined and the perennial species Cichorium intybus L. in all the cultivars of alfalfa. The lowest number of seeds were found in the species Rumex sp. four seeds and only the cultivar NS-Mediana at the site Bačko Gradište I, as with species Conium maculatum seven seeds only in the cultivar K-28 at the site Banatsko Karađorđevo. Most weeds species present (nine) was found in alfalfa cultivars NS-Mediana at the site Bačko Gradište I, and while the lowest number of weeds species (two) was found in the cultivar Banjalučanka at the site Kozarska Dubica., U ovom radu ispitivana je zastupljenost semena različitih korovskih vrsta u naturalnom semenu različitih sorata lucerke sa različitih lokaliteta. Kod svih ispitivanih sorti lucerke i na svim lokalitetima identifikovano je 25 različitih vrsta korova, od toga 14 vrsta su bili višegodišnji korovi, dok su ostalih 11 bile jednogodišnje korovske vrste. Od opasnih višegodišnjih korova koji se razmnožavaju generativnim i vegetativnim putem identifikovani su (Sorghum halepense i Cirsium arvense). Najveći broj semena korova imala je jednogodišnja vrsta Picris echioides L. kod sorte K-22 na lokalitetu Ratari (446). Takođe veliki broj semena utvrđen je i kod višegodišnje vrste Cichorium intybus L. i to kod svih ispitivanih sorata lucerke. Najmanji broj semena utvrđen je kod vrste Rumex sp. (četiri semena) i to jedino kod sorte NS-Mediana na lokalitetu Bačko Gradište I, kao i kod vrste Conium maculatum (sedam semena) samo kod sorte K-28 na lokalitetu Banatsko Karađorđevo. Najviše prisutnih korovskih vrsta (devet) utvrđeno je kod sorte lucerke NS-Mediana na lokalitetu Bačko Gradište I, dok je najmanji broj korovskih vrsta (dve) konstatovan kod sorte Banjalučanka na lokalitetu Kozarska Dubica.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "The distribution of weeds in natural alfalfa seeds, Zastupljenost korova u naturalnom semenu lucerke",
pages = "90-85",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402085S"
}
Štrbanović, R., Poštić, D., Stanisavljević, R., Đukanović, L., Ivanović, Ž., Vasić, T.,& Dolovac, N.. (2014). The distribution of weeds in natural alfalfa seeds. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(2), 85-90.
https://doi.org/10.5937/zasbilj1402085S
Štrbanović R, Poštić D, Stanisavljević R, Đukanović L, Ivanović Ž, Vasić T, Dolovac N. The distribution of weeds in natural alfalfa seeds. in Zaštita bilja. 2014;65(2):85-90.
doi:10.5937/zasbilj1402085S .
Štrbanović, Ratibor, Poštić, Dobrivoj, Stanisavljević, Rade, Đukanović, Lana, Ivanović, Žarko, Vasić, Tanja, Dolovac, Nenad, "The distribution of weeds in natural alfalfa seeds" in Zaštita bilja, 65, no. 2 (2014):85-90,
https://doi.org/10.5937/zasbilj1402085S . .
2

Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method

Gavrilović, Veljko; Stanisavljević, Rade; Stošić, Stefan; Stevanović, Miloš; Aleksić, Goran; Stajić, Milica; Dolovac, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stanisavljević, Rade
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Aleksić, Goran
AU  - Stajić, Milica
AU  - Dolovac, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/301
AB  - Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria.
AB  - U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method
T1  - Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova
EP  - 123
IS  - 3
SP  - 117
VL  - 65
DO  - 10.5937/zasbilj1403117G
ER  - 
@article{
author = "Gavrilović, Veljko and Stanisavljević, Rade and Stošić, Stefan and Stevanović, Miloš and Aleksić, Goran and Stajić, Milica and Dolovac, Nenad",
year = "2014",
abstract = "Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria., U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method, Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova",
pages = "123-117",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403117G"
}
Gavrilović, V., Stanisavljević, R., Stošić, S., Stevanović, M., Aleksić, G., Stajić, M.,& Dolovac, N.. (2014). Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 117-123.
https://doi.org/10.5937/zasbilj1403117G
Gavrilović V, Stanisavljević R, Stošić S, Stevanović M, Aleksić G, Stajić M, Dolovac N. Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja. 2014;65(3):117-123.
doi:10.5937/zasbilj1403117G .
Gavrilović, Veljko, Stanisavljević, Rade, Stošić, Stefan, Stevanović, Miloš, Aleksić, Goran, Stajić, Milica, Dolovac, Nenad, "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method" in Zaštita bilja, 65, no. 3 (2014):117-123,
https://doi.org/10.5937/zasbilj1403117G . .

The effectiveness of potassium phosphite and captan mixture in controlling Venturia inaequalis in apple orchards

Aleksić, Goran; Popović, Tatjana; Starović, Mira; Kuzmanović, Slobodan; Dolovac, Nenad; Jošić, Dragana; Gavrilović, Veljko

(Institute of Pesticides and Environmental Protection, 2014)

TY  - JOUR
AU  - Aleksić, Goran
AU  - Popović, Tatjana
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
AU  - Dolovac, Nenad
AU  - Jošić, Dragana
AU  - Gavrilović, Veljko
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/364
AB  - Venturia inaequalis is a common apple disease in Serbia and its intensity depends on weather conditions. The most successful method of combating apple scab is to use a lower dose of an active ingredient and increase its effectiveness by adding substances that do not cause environmental damage. The effectiveness of a fungicide mixture of captan and potassium phosphite against V. inaequalis was investigated in apple orchards in 2008 and 2009. A relevant EPPO standard method was used in all trials. Captan 50 WP was used as a standard fungicide. In order to determine the exact conditions marking the onset of apple infection, key meteorological conditions were monitored in the orchards using an automated iMetos weather station and a Lufft electronic instrument. The results showed high efficacy of the tested fungicides which remained similar in both years of investigation. The intensity of leaf infection after treatment with the mixture of captan and potassium phosphite was 0.7-2.4%, while fruit infection was 0.5-3.0%. The efficacy of this mixture ranged from 96.3-97.9% on leaves and 87.4-98.7% on fruits. The standard fungicide Captan 50 WP showed an efficacy ranging from 95.3-96.7% on leaves and from 87.8-99.3% on fruits. No statistically significant differences were found between the fungicide tested and the standard fungicide.
AB  - Venturia inaequalis je široko rasprostranjena bolest jabuke u Srbiji, a njen intenzitet zavisi od vremenskih uslova. Najuspešniji metod borbe protiv čađave krastavosti jabuke je korišćenje manjih doza aktivnih materija uz istovremeno povećavanje efikasnosti dodavanjem supstanci koje ne prouzrokuju štetu u životnoj sredini. Tokom 2008 i 2009 godine, proučavana je efikasnost fungicidne mešavine kaptana i kalijum fosfita protiv V. inaequalis u jabučnjacima. U svim ogledima je korišćen standardni EPPO metod. Captan 50 WP je korišćen kao standardni fungicid. Kako bi se tačno odredili uslovi za početak zaraze na jabuci, praćeni su osnovni meteorološki parametri u voćnjacima pomoću iMetos stanice i elektronskog uređaja Lufft. Rezultati su pokazali visoku efikasnost testiranih fungicida koja je bila slična u obe godine istraživanja. Intenzitet zaraze lista nakon tretmana mešavinom kaptana i kalijum fosfita bio je 0.7-2.4%, dok je zaraženost plodova bila 0.5-3.0%. Efikasnost mešavine bila je 96.3-97.9% na listovima, a 87.4-98.7% na plodovima. Standardni fungicid Captan 50 WP pokazao je efikasnost u opsegu 95.3-96.7% na listovima i 87.8-99.3% na plodovima. Nisu konstatovane statistički značajne razlike između ispitivanog i standardnog fungicida.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - The effectiveness of potassium phosphite and captan mixture in controlling Venturia inaequalis in apple orchards
T1  - Efikasnost mešavine kalijum fosfita i kaptana u suzbijanju Venturia inaequalis u jabučnjacima
EP  - 143
IS  - 2
SP  - 137
VL  - 29
DO  - 10.2298/PIF1402137A
ER  - 
@article{
author = "Aleksić, Goran and Popović, Tatjana and Starović, Mira and Kuzmanović, Slobodan and Dolovac, Nenad and Jošić, Dragana and Gavrilović, Veljko",
year = "2014",
abstract = "Venturia inaequalis is a common apple disease in Serbia and its intensity depends on weather conditions. The most successful method of combating apple scab is to use a lower dose of an active ingredient and increase its effectiveness by adding substances that do not cause environmental damage. The effectiveness of a fungicide mixture of captan and potassium phosphite against V. inaequalis was investigated in apple orchards in 2008 and 2009. A relevant EPPO standard method was used in all trials. Captan 50 WP was used as a standard fungicide. In order to determine the exact conditions marking the onset of apple infection, key meteorological conditions were monitored in the orchards using an automated iMetos weather station and a Lufft electronic instrument. The results showed high efficacy of the tested fungicides which remained similar in both years of investigation. The intensity of leaf infection after treatment with the mixture of captan and potassium phosphite was 0.7-2.4%, while fruit infection was 0.5-3.0%. The efficacy of this mixture ranged from 96.3-97.9% on leaves and 87.4-98.7% on fruits. The standard fungicide Captan 50 WP showed an efficacy ranging from 95.3-96.7% on leaves and from 87.8-99.3% on fruits. No statistically significant differences were found between the fungicide tested and the standard fungicide., Venturia inaequalis je široko rasprostranjena bolest jabuke u Srbiji, a njen intenzitet zavisi od vremenskih uslova. Najuspešniji metod borbe protiv čađave krastavosti jabuke je korišćenje manjih doza aktivnih materija uz istovremeno povećavanje efikasnosti dodavanjem supstanci koje ne prouzrokuju štetu u životnoj sredini. Tokom 2008 i 2009 godine, proučavana je efikasnost fungicidne mešavine kaptana i kalijum fosfita protiv V. inaequalis u jabučnjacima. U svim ogledima je korišćen standardni EPPO metod. Captan 50 WP je korišćen kao standardni fungicid. Kako bi se tačno odredili uslovi za početak zaraze na jabuci, praćeni su osnovni meteorološki parametri u voćnjacima pomoću iMetos stanice i elektronskog uređaja Lufft. Rezultati su pokazali visoku efikasnost testiranih fungicida koja je bila slična u obe godine istraživanja. Intenzitet zaraze lista nakon tretmana mešavinom kaptana i kalijum fosfita bio je 0.7-2.4%, dok je zaraženost plodova bila 0.5-3.0%. Efikasnost mešavine bila je 96.3-97.9% na listovima, a 87.4-98.7% na plodovima. Standardni fungicid Captan 50 WP pokazao je efikasnost u opsegu 95.3-96.7% na listovima i 87.8-99.3% na plodovima. Nisu konstatovane statistički značajne razlike između ispitivanog i standardnog fungicida.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "The effectiveness of potassium phosphite and captan mixture in controlling Venturia inaequalis in apple orchards, Efikasnost mešavine kalijum fosfita i kaptana u suzbijanju Venturia inaequalis u jabučnjacima",
pages = "143-137",
number = "2",
volume = "29",
doi = "10.2298/PIF1402137A"
}
Aleksić, G., Popović, T., Starović, M., Kuzmanović, S., Dolovac, N., Jošić, D.,& Gavrilović, V.. (2014). The effectiveness of potassium phosphite and captan mixture in controlling Venturia inaequalis in apple orchards. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 29(2), 137-143.
https://doi.org/10.2298/PIF1402137A
Aleksić G, Popović T, Starović M, Kuzmanović S, Dolovac N, Jošić D, Gavrilović V. The effectiveness of potassium phosphite and captan mixture in controlling Venturia inaequalis in apple orchards. in Pesticidi i fitomedicina. 2014;29(2):137-143.
doi:10.2298/PIF1402137A .
Aleksić, Goran, Popović, Tatjana, Starović, Mira, Kuzmanović, Slobodan, Dolovac, Nenad, Jošić, Dragana, Gavrilović, Veljko, "The effectiveness of potassium phosphite and captan mixture in controlling Venturia inaequalis in apple orchards" in Pesticidi i fitomedicina, 29, no. 2 (2014):137-143,
https://doi.org/10.2298/PIF1402137A . .

First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia

Živković, Svetlana; Gavrilović, Veljko; Popović, Tatjana; Dolovac, Nenad; Trkulja, Nenad

(Amer Phytopathological Soc, St Paul, 2014)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Popović, Tatjana
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/337
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia
EP  - 1272
IS  - 9
SP  - 1272
VL  - 98
DO  - 10.1094/PDIS-01-14-0052-PDN
ER  - 
@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Popović, Tatjana and Dolovac, Nenad and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia",
pages = "1272-1272",
number = "9",
volume = "98",
doi = "10.1094/PDIS-01-14-0052-PDN"
}
Živković, S., Gavrilović, V., Popović, T., Dolovac, N.,& Trkulja, N.. (2014). First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(9), 1272-1272.
https://doi.org/10.1094/PDIS-01-14-0052-PDN
Živković S, Gavrilović V, Popović T, Dolovac N, Trkulja N. First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease. 2014;98(9):1272-1272.
doi:10.1094/PDIS-01-14-0052-PDN .
Živković, Svetlana, Gavrilović, Veljko, Popović, Tatjana, Dolovac, Nenad, Trkulja, Nenad, "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia" in Plant Disease, 98, no. 9 (2014):1272-1272,
https://doi.org/10.1094/PDIS-01-14-0052-PDN . .
3
1
2

Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management

Dolovac, Nenad; Trkulja, Nenad; Milosavljević, Anja; Pfaf-Dolovac, Erika

(Amer Phytopathological Soc, St Paul, 2014)

TY  - CONF
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/335
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management
EP  - 34
IS  - 11
SP  - 34
VL  - 104
ER  - 
@conference{
author = "Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management",
pages = "34-34",
number = "11",
volume = "104"
}
Dolovac, N., Trkulja, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 34-34.
Dolovac N, Trkulja N, Milosavljević A, Pfaf-Dolovac E. Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology. 2014;104(11):34-34..
Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management" in Phytopathology, 104, no. 11 (2014):34-34.

Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola

Trkulja, Nenad; Dolovac, Nenad; Milosavljević, Anja; Pfaf-Dolovac, Erika

(Amer Phytopathological Soc, St Paul, 2014)

TY  - CONF
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/334
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola
EP  - 120
IS  - 11
SP  - 120
VL  - 104
ER  - 
@conference{
author = "Trkulja, Nenad and Dolovac, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola",
pages = "120-120",
number = "11",
volume = "104"
}
Trkulja, N., Dolovac, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 120-120.
Trkulja N, Dolovac N, Milosavljević A, Pfaf-Dolovac E. Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology. 2014;104(11):120-120..
Trkulja, Nenad, Dolovac, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola" in Phytopathology, 104, no. 11 (2014):120-120.

First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia

Jošić, Dragana; Starović, Mira; Stojanović, Saša; Popović Milovanović, Tatjana; Dolovac, Nenad; Zdravković, Jasmina; Pavlović, Snežana

(American Phytopathological Society, 2013)


                                            

                                            
Jošić, D., Starović, M., Stojanović, S., Popović Milovanović, T., Dolovac, N., Zdravković, J.,& Pavlović, S.. (2013). First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia. in Plant Disease
American Phytopathological Society., 97(3), 420.
https://doi.org/10.1094/PDIS-09-12-0885-PDN
Jošić D, Starović M, Stojanović S, Popović Milovanović T, Dolovac N, Zdravković J, Pavlović S. First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia. in Plant Disease. 2013;97(3):420.
doi:10.1094/PDIS-09-12-0885-PDN .
Jošić, Dragana, Starović, Mira, Stojanović, Saša, Popović Milovanović, Tatjana, Dolovac, Nenad, Zdravković, Jasmina, Pavlović, Snežana, "First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia" in Plant Disease, 97, no. 3 (2013):420,
https://doi.org/10.1094/PDIS-09-12-0885-PDN . .
2
1
2

Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia

Labus Blagojevic, Svetlana; Gavrilovic, Veljko; Dolovac, Nenad; Pfaf Dolovac, Erika; Trkulja, Nenad

(Serbian Water Pollution Control Society, 2013)

TY  - JOUR
AU  - Labus Blagojevic, Svetlana
AU  - Gavrilovic, Veljko
AU  - Dolovac, Nenad
AU  - Pfaf Dolovac, Erika
AU  - Trkulja, Nenad
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1140
PB  - Serbian Water Pollution Control Society
T2  - Water Research and Management
T1  - Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia
EP  - 50
IS  - 3
SP  - 37
VL  - 3
ER  - 
@article{
author = "Labus Blagojevic, Svetlana and Gavrilovic, Veljko and Dolovac, Nenad and Pfaf Dolovac, Erika and Trkulja, Nenad",
year = "2013",
publisher = "Serbian Water Pollution Control Society",
journal = "Water Research and Management",
title = "Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia",
pages = "50-37",
number = "3",
volume = "3"
}
Labus Blagojevic, S., Gavrilovic, V., Dolovac, N., Pfaf Dolovac, E.,& Trkulja, N.. (2013). Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia. in Water Research and Management
Serbian Water Pollution Control Society., 3(3), 37-50.
Labus Blagojevic S, Gavrilovic V, Dolovac N, Pfaf Dolovac E, Trkulja N. Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia. in Water Research and Management. 2013;3(3):37-50..
Labus Blagojevic, Svetlana, Gavrilovic, Veljko, Dolovac, Nenad, Pfaf Dolovac, Erika, Trkulja, Nenad, "Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia" in Water Research and Management, 3, no. 3 (2013):37-50.

Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke

Štrbanović, Ratibor; Gavrilović, Veljko; Stanisavljević, Rade; Poštić, Dobrivoj; Marković, Jordan; Trkulja, Nenad; Dolovac, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2013)

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Gavrilović, Veljko
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Marković, Jordan
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/256
AB  - In this study we investigated the presence of plant pathogenic genera of fungi on seeds of three different cultivars of alfalfa (K-22, NS-Banat and OS-88) with three different lots (locality) of each cultivars. When tested cultivars were identified following genera of fungi: Alternaria spp ., Fusarium spp., Penicillium spp., Mucor spp. and sterile mycelium. The presence of the identified genera of fungi ranged from 0% to 2%. Genus Alternaria spp. lowest average value was in alfalfa cultivars K-22 (0.42%), while the highest average value of a cultivar OS-88 (0.58 %), and also at the same cultivar was noted by the presence of one (1%) at the locality of Osijek I. In all the cultivars of alfalfa, a high coefficient of variation, indicating a high variability within the three cultivars for this trait. The highest average attendance of the genus Fusarium spp. was observed in cultivar OS-88 (0.75%), and in the same cultivar is the largest presence (1.25%) were recorded at the locality of Osijek II. For the studied alfalfa cultivars were examined correlations between parameters of seed quality and the presence of pathogens on seeds of different cultivars of alfalfa and lots. Strong positive correlations were observed between germination energy and total germi­nation (r=0.891***), and between mass of 1000 seeds and the presence of Fusarium spp. (r=0.797**). The results of these tests indicate satisfactory state of health of all cultivars and alfalfa seed lots and in particular in relation to the economically important phytopathogenic fungi of the genus Fusarium spp.
AB  - U ovom radu je ispitivano prisustvo fitopatogenih rodova gljiva na semenu tri različite sorte lucerke (K-22, NS-Banat i OS-88) sa po tri različite partije (lokaliteta) od svake sorte. Kod ispitivanih sorti identifikovani su sledeći rodovi gljiva: Alternaria spp., Fusarium spp., Penicillium spp., Mucor spp. i sterilna micelija. Prisustvo identifikovanih rodova gljiva kretalo se u rasponu od 0% do 2%. Rod Alternaria spp. najmanju prosečnu vrednost imao je kod sorte lucerke K-22 (0.42%), dok je najveću prosečnu vrednost imao kod sorte OS-88 (0.58%), a takođe kod iste sorte konstatovano je najveće prisustvo od (1%) na lokalitetu Osijek I. Kod svih ispitivanih sorata lucerke utvrđen je visok koeficijent varijacije, što ukazuje na visoku varijabilnost unutar samih sorata za ovo svojstvo. Najveće prosečno prisustvo gljiva roda Fusarium spp. zabeleženo je kod sorte OS-88 (0.75%), a kod iste sorte je najveće prisustvo (1.25%) zabeleženo na lokalitetu Osijek II. Na proučavanim sortama lucerke ispitani su korelacioni odnosi između parametara kvaliteta semena i prisustva patogena na semenu različitih sorata i partija lucerke. Jake pozitivne korelacije zabeležene su između energije klijanja i ukupne klijavosti (r=0,891***), kao i između mase 1000 semena i prisustva gljiva iz roda Fusarium spp. (r=0,797**). Rezultati ovih ispitivanja ukazuju na zadovoljavajuće zdravstveno stanje svih sorata i partija semena lucerke a posebno u odnosu na ekonomski značajne fitopatogene gljive iz roda Fusarium spp.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke
T1  - Health testing different genotypes alfalfa seeds
EP  - 217
IS  - 4
SP  - 212
VL  - 64
ER  - 
@article{
author = "Štrbanović, Ratibor and Gavrilović, Veljko and Stanisavljević, Rade and Poštić, Dobrivoj and Marković, Jordan and Trkulja, Nenad and Dolovac, Nenad",
year = "2013",
abstract = "In this study we investigated the presence of plant pathogenic genera of fungi on seeds of three different cultivars of alfalfa (K-22, NS-Banat and OS-88) with three different lots (locality) of each cultivars. When tested cultivars were identified following genera of fungi: Alternaria spp ., Fusarium spp., Penicillium spp., Mucor spp. and sterile mycelium. The presence of the identified genera of fungi ranged from 0% to 2%. Genus Alternaria spp. lowest average value was in alfalfa cultivars K-22 (0.42%), while the highest average value of a cultivar OS-88 (0.58 %), and also at the same cultivar was noted by the presence of one (1%) at the locality of Osijek I. In all the cultivars of alfalfa, a high coefficient of variation, indicating a high variability within the three cultivars for this trait. The highest average attendance of the genus Fusarium spp. was observed in cultivar OS-88 (0.75%), and in the same cultivar is the largest presence (1.25%) were recorded at the locality of Osijek II. For the studied alfalfa cultivars were examined correlations between parameters of seed quality and the presence of pathogens on seeds of different cultivars of alfalfa and lots. Strong positive correlations were observed between germination energy and total germi­nation (r=0.891***), and between mass of 1000 seeds and the presence of Fusarium spp. (r=0.797**). The results of these tests indicate satisfactory state of health of all cultivars and alfalfa seed lots and in particular in relation to the economically important phytopathogenic fungi of the genus Fusarium spp., U ovom radu je ispitivano prisustvo fitopatogenih rodova gljiva na semenu tri različite sorte lucerke (K-22, NS-Banat i OS-88) sa po tri različite partije (lokaliteta) od svake sorte. Kod ispitivanih sorti identifikovani su sledeći rodovi gljiva: Alternaria spp., Fusarium spp., Penicillium spp., Mucor spp. i sterilna micelija. Prisustvo identifikovanih rodova gljiva kretalo se u rasponu od 0% do 2%. Rod Alternaria spp. najmanju prosečnu vrednost imao je kod sorte lucerke K-22 (0.42%), dok je najveću prosečnu vrednost imao kod sorte OS-88 (0.58%), a takođe kod iste sorte konstatovano je najveće prisustvo od (1%) na lokalitetu Osijek I. Kod svih ispitivanih sorata lucerke utvrđen je visok koeficijent varijacije, što ukazuje na visoku varijabilnost unutar samih sorata za ovo svojstvo. Najveće prosečno prisustvo gljiva roda Fusarium spp. zabeleženo je kod sorte OS-88 (0.75%), a kod iste sorte je najveće prisustvo (1.25%) zabeleženo na lokalitetu Osijek II. Na proučavanim sortama lucerke ispitani su korelacioni odnosi između parametara kvaliteta semena i prisustva patogena na semenu različitih sorata i partija lucerke. Jake pozitivne korelacije zabeležene su između energije klijanja i ukupne klijavosti (r=0,891***), kao i između mase 1000 semena i prisustva gljiva iz roda Fusarium spp. (r=0,797**). Rezultati ovih ispitivanja ukazuju na zadovoljavajuće zdravstveno stanje svih sorata i partija semena lucerke a posebno u odnosu na ekonomski značajne fitopatogene gljive iz roda Fusarium spp.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke, Health testing different genotypes alfalfa seeds",
pages = "217-212",
number = "4",
volume = "64"
}
Štrbanović, R., Gavrilović, V., Stanisavljević, R., Poštić, D., Marković, J., Trkulja, N.,& Dolovac, N.. (2013). Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(4), 212-217.
Štrbanović R, Gavrilović V, Stanisavljević R, Poštić D, Marković J, Trkulja N, Dolovac N. Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke. in Zaštita bilja. 2013;64(4):212-217..
Štrbanović, Ratibor, Gavrilović, Veljko, Stanisavljević, Rade, Poštić, Dobrivoj, Marković, Jordan, Trkulja, Nenad, Dolovac, Nenad, "Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke" in Zaštita bilja, 64, no. 4 (2013):212-217.

Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli

Popović, Tatjana; Jošić, Dragana; Starović, Mira; Milovanović, Predrag; Dolovac, Nenad; Poštić, Dobrivoj; Stanković, Slaviša

(University of Belgrade, University of Novi Sad, 2013)

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Milovanović, Predrag
AU  - Dolovac, Nenad
AU  - Poštić, Dobrivoj
AU  - Stanković, Slaviša
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/279
AB  - Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35°C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli
EP  - 593
IS  - 2
SP  - 585
VL  - 65
DO  - 10.2298/ABS1302585P
ER  - 
@article{
author = "Popović, Tatjana and Jošić, Dragana and Starović, Mira and Milovanović, Predrag and Dolovac, Nenad and Poštić, Dobrivoj and Stanković, Slaviša",
year = "2013",
abstract = "Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35°C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli",
pages = "593-585",
number = "2",
volume = "65",
doi = "10.2298/ABS1302585P"
}
Popović, T., Jošić, D., Starović, M., Milovanović, P., Dolovac, N., Poštić, D.,& Stanković, S.. (2013). Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 65(2), 585-593.
https://doi.org/10.2298/ABS1302585P
Popović T, Jošić D, Starović M, Milovanović P, Dolovac N, Poštić D, Stanković S. Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli. in Archives of Biological Sciences. 2013;65(2):585-593.
doi:10.2298/ABS1302585P .
Popović, Tatjana, Jošić, Dragana, Starović, Mira, Milovanović, Predrag, Dolovac, Nenad, Poštić, Dobrivoj, Stanković, Slaviša, "Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli" in Archives of Biological Sciences, 65, no. 2 (2013):585-593,
https://doi.org/10.2298/ABS1302585P . .
7
5
8

Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene

Trkulja, Nenad; Ivanović, Žarko; Pfaf-Dolovac, Erika; Dolovac, Nenad; Mitrović, Milana; Toševski, Ivo; Jović, Jelena

(Springer, Dordrecht, 2013)

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Ivanović, Žarko
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/272
AB  - A survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene
EP  - 902
IS  - 4
SP  - 889
VL  - 135
DO  - 10.1007/s10658-012-0135-x
ER  - 
@article{
author = "Trkulja, Nenad and Ivanović, Žarko and Pfaf-Dolovac, Erika and Dolovac, Nenad and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2013",
abstract = "A survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene",
pages = "902-889",
number = "4",
volume = "135",
doi = "10.1007/s10658-012-0135-x"
}
Trkulja, N., Ivanović, Ž., Pfaf-Dolovac, E., Dolovac, N., Mitrović, M., Toševski, I.,& Jović, J.. (2013). Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene. in European Journal of Plant Pathology
Springer, Dordrecht., 135(4), 889-902.
https://doi.org/10.1007/s10658-012-0135-x
Trkulja N, Ivanović Ž, Pfaf-Dolovac E, Dolovac N, Mitrović M, Toševski I, Jović J. Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene. in European Journal of Plant Pathology. 2013;135(4):889-902.
doi:10.1007/s10658-012-0135-x .
Trkulja, Nenad, Ivanović, Žarko, Pfaf-Dolovac, Erika, Dolovac, Nenad, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene" in European Journal of Plant Pathology, 135, no. 4 (2013):889-902,
https://doi.org/10.1007/s10658-012-0135-x . .
23
14
24

First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia

Jošić, Dragana; Starović, Mira; Stojanović, S.; Popović, Tatjana; Dolovac, Nenad; Zdravković, Jasmina; Pavlović, Snežana

(Amer Phytopathological Soc, St Paul, 2013)

TY  - JOUR
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Stojanović, S.
AU  - Popović, Tatjana
AU  - Dolovac, Nenad
AU  - Zdravković, Jasmina
AU  - Pavlović, Snežana
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/288
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia
EP  - 420
IS  - 3
SP  - 420
VL  - 97
DO  - 10.1094/PDIS-09-12-0885-PDN
ER  - 
@article{
author = "Jošić, Dragana and Starović, Mira and Stojanović, S. and Popović, Tatjana and Dolovac, Nenad and Zdravković, Jasmina and Pavlović, Snežana",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia",
pages = "420-420",
number = "3",
volume = "97",
doi = "10.1094/PDIS-09-12-0885-PDN"
}
Jošić, D., Starović, M., Stojanović, S., Popović, T., Dolovac, N., Zdravković, J.,& Pavlović, S.. (2013). First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(3), 420-420.
https://doi.org/10.1094/PDIS-09-12-0885-PDN
Jošić D, Starović M, Stojanović S, Popović T, Dolovac N, Zdravković J, Pavlović S. First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia. in Plant Disease. 2013;97(3):420-420.
doi:10.1094/PDIS-09-12-0885-PDN .
Jošić, Dragana, Starović, Mira, Stojanović, S., Popović, Tatjana, Dolovac, Nenad, Zdravković, Jasmina, Pavlović, Snežana, "First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia" in Plant Disease, 97, no. 3 (2013):420-420,
https://doi.org/10.1094/PDIS-09-12-0885-PDN . .
2
1
2

First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia

Stevanović, Miloš; Stanković, Ivana; Vučurović, Ana; Dolovac, Nenad; Pfaf-Dolovac, Erika; Krstić, Branka; Bulajić, Aleksandra

(Amer Phytopathological Soc, St Paul, 2012)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Dolovac, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/216
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia
EP  - 912
IS  - 6
SP  - 912
VL  - 96
DO  - 10.1094/PDIS-02-12-0179-PDN
ER  - 
@article{
author = "Stevanović, Miloš and Stanković, Ivana and Vučurović, Ana and Dolovac, Nenad and Pfaf-Dolovac, Erika and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia",
pages = "912-912",
number = "6",
volume = "96",
doi = "10.1094/PDIS-02-12-0179-PDN"
}
Stevanović, M., Stanković, I., Vučurović, A., Dolovac, N., Pfaf-Dolovac, E., Krstić, B.,& Bulajić, A.. (2012). First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(6), 912-912.
https://doi.org/10.1094/PDIS-02-12-0179-PDN
Stevanović M, Stanković I, Vučurović A, Dolovac N, Pfaf-Dolovac E, Krstić B, Bulajić A. First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease. 2012;96(6):912-912.
doi:10.1094/PDIS-02-12-0179-PDN .
Stevanović, Miloš, Stanković, Ivana, Vučurović, Ana, Dolovac, Nenad, Pfaf-Dolovac, Erika, Krstić, Branka, Bulajić, Aleksandra, "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia" in Plant Disease, 96, no. 6 (2012):912-912,
https://doi.org/10.1094/PDIS-02-12-0179-PDN . .
4

Etiology of diseases of some medicinal plants in plantation in Serbia

Pavlović, Snežana; Stojanović, Saša; Kuzmanović, Slobodan; Starović, Mira; Živković, Svetlana; Dolovac, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Stojanović, Saša
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/238
AB  - Marshmallow, coneflower, St. John's wort and sage, which grown in plantation, were the hosts of numerous fungi species that cause a variety of symptoms. A total of 34 species from 22 genera were determined. Some species pathogen on only one host (Puccinia malvacearum on marshmallow, Diaporthe eres and Seimatosporium hypericum on St. John's wort), while others were present at all examined hosts (species of the genus Fusarium are present in all examined hosts). The most abundant species in the seed was Alternaria alternate. The percentage of contaminated seeds ranged from 5% to 69%. Fusaium species were isolated from 2.5% to 29% of seeds, depending on the host and year. From the root of the studied medicinal plants seven species of Fusarium (F.graminearum, F. oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans and F.verticilioides) and one of the genus Sclerotinia (S.sclerotiorum) were isolated. Thirteen fungi species belonging to 10 genera were isolated from stem, leaves and flower of medicinal plants.
AB  - Plantažno gajeni beli slez, ehinacee, kantarion i žalfija su domaćini brojnih vrsta gljiva koje prouzrokuju različite simptome. Ukupno je determinisano 34 vrsta iz 22 roda. Neke vrste parazitiraju samo jednog domaćina (Puccinia malvacearum na belom slezu, Diaporthe eres i Seimatosporium hypericum na kantarionu), dok druge ispoljavaju izrazitu polifagnost (vrste iz roda Fusarium prisutne su na svim ispitivanim domaćinima). Najzastupljenija vrsta na semenu bila je Alternaria alternata, koja je uvek prisutna na semenu u visokom procentu, a pojednih godina i preko 60 %. Procenat kontaminiranog semana kretao se od 5% do 69%. godini. U proseku procenat zaraženog semena sa vrstama roda Fusaium bio 17,2% i kretao se od 2,5% do 29% u zavisnosti od domaćina i godine. Na korenu ispitivanih lekovitih biljaka izolovano je sedam vrsta gljiva iz roda Fusarium (F.graminearum, F.oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans i F verticilioides) i jedna iz roda Sclerotinia (S.sclerotiorum). Sa nadzemnih organa izolovano je 13 vrsta gljiva iz 10 rodova.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Etiology of diseases of some medicinal plants in plantation in Serbia
T1  - Etiologija oboljenja važnijih plantažno gajenih lekovitih biljaka u Srbiji
EP  - 241
IS  - 4
SP  - 224
VL  - 63
ER  - 
@article{
author = "Pavlović, Snežana and Stojanović, Saša and Kuzmanović, Slobodan and Starović, Mira and Živković, Svetlana and Dolovac, Nenad",
year = "2012",
abstract = "Marshmallow, coneflower, St. John's wort and sage, which grown in plantation, were the hosts of numerous fungi species that cause a variety of symptoms. A total of 34 species from 22 genera were determined. Some species pathogen on only one host (Puccinia malvacearum on marshmallow, Diaporthe eres and Seimatosporium hypericum on St. John's wort), while others were present at all examined hosts (species of the genus Fusarium are present in all examined hosts). The most abundant species in the seed was Alternaria alternate. The percentage of contaminated seeds ranged from 5% to 69%. Fusaium species were isolated from 2.5% to 29% of seeds, depending on the host and year. From the root of the studied medicinal plants seven species of Fusarium (F.graminearum, F. oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans and F.verticilioides) and one of the genus Sclerotinia (S.sclerotiorum) were isolated. Thirteen fungi species belonging to 10 genera were isolated from stem, leaves and flower of medicinal plants., Plantažno gajeni beli slez, ehinacee, kantarion i žalfija su domaćini brojnih vrsta gljiva koje prouzrokuju različite simptome. Ukupno je determinisano 34 vrsta iz 22 roda. Neke vrste parazitiraju samo jednog domaćina (Puccinia malvacearum na belom slezu, Diaporthe eres i Seimatosporium hypericum na kantarionu), dok druge ispoljavaju izrazitu polifagnost (vrste iz roda Fusarium prisutne su na svim ispitivanim domaćinima). Najzastupljenija vrsta na semenu bila je Alternaria alternata, koja je uvek prisutna na semenu u visokom procentu, a pojednih godina i preko 60 %. Procenat kontaminiranog semana kretao se od 5% do 69%. godini. U proseku procenat zaraženog semena sa vrstama roda Fusaium bio 17,2% i kretao se od 2,5% do 29% u zavisnosti od domaćina i godine. Na korenu ispitivanih lekovitih biljaka izolovano je sedam vrsta gljiva iz roda Fusarium (F.graminearum, F.oxysporum, F.proliferatum, F.semitectum, F.solani, F.subglutinans i F verticilioides) i jedna iz roda Sclerotinia (S.sclerotiorum). Sa nadzemnih organa izolovano je 13 vrsta gljiva iz 10 rodova.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Etiology of diseases of some medicinal plants in plantation in Serbia, Etiologija oboljenja važnijih plantažno gajenih lekovitih biljaka u Srbiji",
pages = "241-224",
number = "4",
volume = "63"
}
Pavlović, S., Stojanović, S., Kuzmanović, S., Starović, M., Živković, S.,& Dolovac, N.. (2012). Etiology of diseases of some medicinal plants in plantation in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(4), 224-241.
Pavlović S, Stojanović S, Kuzmanović S, Starović M, Živković S, Dolovac N. Etiology of diseases of some medicinal plants in plantation in Serbia. in Zaštita bilja. 2012;63(4):224-241..
Pavlović, Snežana, Stojanović, Saša, Kuzmanović, Slobodan, Starović, Mira, Živković, Svetlana, Dolovac, Nenad, "Etiology of diseases of some medicinal plants in plantation in Serbia" in Zaštita bilja, 63, no. 4 (2012):224-241.

Morphology of two new Globodera rostochiensis populations from Serbia

Oro, Violeta; Živković, Svetlana; Dolovac, Nenad; Kuzmanović, Slobodan; Ivanović, Žarko

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Oro, Violeta
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
AU  - Kuzmanović, Slobodan
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/244
AB  - In concordance with phytosanitary regulations and the Program of continuous monitoring of quarantine and economically harmful nematodes that are intensively conducted every year, the presence of these organisms is determined in the territory of the Republic of Serbia and in the samples of plant material. Globodera pallida and G.rostochiensis are the most important parasites of potato in our country and their presence is monitored since 2005 and 2000 respectively. Morphology, i.e. examination of morphological characters of different populations of potato cyst nematodes determines their mutual similarities (differences) within species, between species and the similarity with the type populations. This paper presents the morphological descriptions of cysts and second stage larvae of two populations of G.rostochiensis found in localities Rujevac and Tabanovici in 2012.
AB  - U skladu sa Programom stalnog praćenja karantinskih i ekonomski štetnih nematoda koji se intenzivno sprovodi svake godine kao i fitosanitarnom regulativom, utvrđuje se prisustvo ovih organizama na teritoriji Republike Srbije i u uzorcima biljnog materijala. Globodera pallida i G. rostochiensis su najznačajniji paraziti krompira kod nas a njihovo prisustvo se prati od 2005. odnosno 2000. god. Morfologija odnosno ispitivanje morfoloških karakteristika različitih populacija cistolikih nematoda krompira daje podatke o njihovoj međusobnoj sličnosti (razlikama) unutar vrsta, između vrsta i sličnost sa tipskim populacijama. U radu su dati morfološki opisi cisti i larvi drugog stepena dve populacije G. rostochiensis pronadjene na lokalitetima Rujevac i Tabanovići u 2012. godini.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphology of two new Globodera rostochiensis populations from Serbia
T1  - Morfologija dve nove populacije Globodera rostochiensis iz Srbije
EP  - 129
IS  - 3
SP  - 123
VL  - 63
ER  - 
@article{
author = "Oro, Violeta and Živković, Svetlana and Dolovac, Nenad and Kuzmanović, Slobodan and Ivanović, Žarko",
year = "2012",
abstract = "In concordance with phytosanitary regulations and the Program of continuous monitoring of quarantine and economically harmful nematodes that are intensively conducted every year, the presence of these organisms is determined in the territory of the Republic of Serbia and in the samples of plant material. Globodera pallida and G.rostochiensis are the most important parasites of potato in our country and their presence is monitored since 2005 and 2000 respectively. Morphology, i.e. examination of morphological characters of different populations of potato cyst nematodes determines their mutual similarities (differences) within species, between species and the similarity with the type populations. This paper presents the morphological descriptions of cysts and second stage larvae of two populations of G.rostochiensis found in localities Rujevac and Tabanovici in 2012., U skladu sa Programom stalnog praćenja karantinskih i ekonomski štetnih nematoda koji se intenzivno sprovodi svake godine kao i fitosanitarnom regulativom, utvrđuje se prisustvo ovih organizama na teritoriji Republike Srbije i u uzorcima biljnog materijala. Globodera pallida i G. rostochiensis su najznačajniji paraziti krompira kod nas a njihovo prisustvo se prati od 2005. odnosno 2000. god. Morfologija odnosno ispitivanje morfoloških karakteristika različitih populacija cistolikih nematoda krompira daje podatke o njihovoj međusobnoj sličnosti (razlikama) unutar vrsta, između vrsta i sličnost sa tipskim populacijama. U radu su dati morfološki opisi cisti i larvi drugog stepena dve populacije G. rostochiensis pronadjene na lokalitetima Rujevac i Tabanovići u 2012. godini.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphology of two new Globodera rostochiensis populations from Serbia, Morfologija dve nove populacije Globodera rostochiensis iz Srbije",
pages = "129-123",
number = "3",
volume = "63"
}
Oro, V., Živković, S., Dolovac, N., Kuzmanović, S.,& Ivanović, Ž.. (2012). Morphology of two new Globodera rostochiensis populations from Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 123-129.
Oro V, Živković S, Dolovac N, Kuzmanović S, Ivanović Ž. Morphology of two new Globodera rostochiensis populations from Serbia. in Zaštita bilja. 2012;63(3):123-129..
Oro, Violeta, Živković, Svetlana, Dolovac, Nenad, Kuzmanović, Slobodan, Ivanović, Žarko, "Morphology of two new Globodera rostochiensis populations from Serbia" in Zaštita bilja, 63, no. 3 (2012):123-129.