TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Starović, Mira
AU  - Popović, Tatjana
AU  - Bosnić, Predrag
AU  - Stanojković-Sebić, Aleksandra
AU  - Pivić, Radmila
AU  - Jošić, Dragana
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/253
AB  - Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µ gmL- 1 after 24h of cultivation and 4.08 to 26.4 µ gmL-1 after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability.
AB  - Izolati Pseudomonas iz rizosfere kukuruza (Q4 i Q20), lucerke (Q1 i Q16) i divlje bele deteline (B25) selektovani su na osnovu produkcije indolsirćetne kiseline (IAA) radi ispitivanja efekta na biološku sposobnosti semenskih krtola krompira. Produkcija IAA iznosila je 4.09 do 15.9 µ gmL-1 posle 24h i 4.08 do 26.4 µ gmL-1 posle 48h kultivacije. Upoređivanje izolata izvršeno je na osnovu RAPD analize. Na osnovu RAPD profila selektovanih Pseudomonas spp. izolata, dobijenih amplifikacijom BC318, AF14, SPH1 i AP10 prajmerima, ustanovljen je visok stepen različitosti između 4 izolata (44 do 68%), što je potvrdilo efikasnost RAPD metode pri upoređivanju izolata. Efekat na biološku sposobnosti semenskih krtola krompira (industrijski važnu sortu Pirol) ispitivan je tokom sedam nedelja naklijavanja na temperaturi 18-20°C. Krtole tretirane selektovanim izolatima bakterija formirale su nešto manji broj klica, ali je dužina klica statistički značajno veća i do 129,9% u odnosu na kontrolu. Ostvaren je kapacitet klijanja veći do 64,6% u odnosu na kontrolu. Dobijeni rezultati ukazuju na pozitivan efekat selektovanih Pseudomonas izolata koji produkuju IAA na dužinu klica i kapacitet klijanja kao parametara koji određuju biološku sposobnosti semenskih krtola krompira.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers
T1  - Selekcija i RAPD analiza izolata Pseudomonas spp. koji poboljšavaju biološku sposobnost krtola krompira
EP  - 249
IS  - 1
SP  - 237
VL  - 45
DO  - 10.2298/GENSR1301237P
ER  - 

@article{
author = "Poštić, Dobrivoj and Starović, Mira and Popović, Tatjana and Bosnić, Predrag and Stanojković-Sebić, Aleksandra and Pivić, Radmila and Jošić, Dragana",
year = "2013",
abstract = "Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µ gmL- 1 after 24h of cultivation and 4.08 to 26.4 µ gmL-1 after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability., Izolati Pseudomonas iz rizosfere kukuruza (Q4 i Q20), lucerke (Q1 i Q16) i divlje bele deteline (B25) selektovani su na osnovu produkcije indolsirćetne kiseline (IAA) radi ispitivanja efekta na biološku sposobnosti semenskih krtola krompira. Produkcija IAA iznosila je 4.09 do 15.9 µ gmL-1 posle 24h i 4.08 do 26.4 µ gmL-1 posle 48h kultivacije. Upoređivanje izolata izvršeno je na osnovu RAPD analize. Na osnovu RAPD profila selektovanih Pseudomonas spp. izolata, dobijenih amplifikacijom BC318, AF14, SPH1 i AP10 prajmerima, ustanovljen je visok stepen različitosti između 4 izolata (44 do 68%), što je potvrdilo efikasnost RAPD metode pri upoređivanju izolata. Efekat na biološku sposobnosti semenskih krtola krompira (industrijski važnu sortu Pirol) ispitivan je tokom sedam nedelja naklijavanja na temperaturi 18-20°C. Krtole tretirane selektovanim izolatima bakterija formirale su nešto manji broj klica, ali je dužina klica statistički značajno veća i do 129,9% u odnosu na kontrolu. Ostvaren je kapacitet klijanja veći do 64,6% u odnosu na kontrolu. Dobijeni rezultati ukazuju na pozitivan efekat selektovanih Pseudomonas izolata koji produkuju IAA na dužinu klica i kapacitet klijanja kao parametara koji određuju biološku sposobnosti semenskih krtola krompira.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers, Selekcija i RAPD analiza izolata Pseudomonas spp. koji poboljšavaju biološku sposobnost krtola krompira",
pages = "249-237",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301237P"
}

Poštić, D., Starović, M., Popović, T., Bosnić, P., Stanojković-Sebić, A., Pivić, R.,& Jošić, D.. (2013). Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers. in Genetika-Belgrade
Serbian Genetics Society., 45(1), 237-249.
https://doi.org/10.2298/GENSR1301237P

Poštić D, Starović M, Popović T, Bosnić P, Stanojković-Sebić A, Pivić R, Jošić D. Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers. in Genetika-Belgrade. 2013;45(1):237-249.
doi:10.2298/GENSR1301237P .

Poštić, Dobrivoj, Starović, Mira, Popović, Tatjana, Bosnić, Predrag, Stanojković-Sebić, Aleksandra, Pivić, Radmila, Jošić, Dragana, "Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers" in Genetika-Belgrade, 45, no. 1 (2013):237-249,
https://doi.org/10.2298/GENSR1301237P . .