Stevanović, Miloš

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  • Stevanović, Miloš (15)
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Author's Bibliography

Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia

Stevanović, Miloš; Ristić, Danijela; Živković, Svetlana; Aleksić, Goran; Stanković, Ivana; Krstić, Branka; Bulajić, Aleksandra

(Amer Phytopathological Soc, St Paul, 2019)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Ristić, Danijela
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/570
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 
@article{
author = "Stevanović, Miloš and Ristić, Danijela and Živković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}
Stevanović, M., Ristić, D., Živković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE
Stevanović M, Ristić D, Živković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .
Stevanović, Miloš, Ristić, Danijela, Živković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .
7
2
7

Efficacy of copper citrate in grapevine disease control

Aleksić, Goran; Milićević, Zoran; Kuzmanović, Slobodan; Starović, Mira; Stevanović, Miloš; Delibašić, Goran; Živković, Svetlana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2019)

TY  - JOUR
AU  - Aleksić, Goran
AU  - Milićević, Zoran
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Delibašić, Goran
AU  - Živković, Svetlana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5229
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/599
AB  - The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection.
AB  - Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Efficacy of copper citrate in grapevine disease control
T1  - Efikasnost bakar-citrata u zaštiti vinove loze od bolesti
EP  - 109
IS  - 2
SP  - 103
VL  - 34
ER  - 
@article{
author = "Aleksić, Goran and Milićević, Zoran and Kuzmanović, Slobodan and Starović, Mira and Stevanović, Miloš and Delibašić, Goran and Živković, Svetlana",
year = "2019",
abstract = "The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection., Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Efficacy of copper citrate in grapevine disease control, Efikasnost bakar-citrata u zaštiti vinove loze od bolesti",
pages = "109-103",
number = "2",
volume = "34"
}
Aleksić, G., Milićević, Z., Kuzmanović, S., Starović, M., Stevanović, M., Delibašić, G.,& Živković, S.. (2019). Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(2), 103-109.
Aleksić G, Milićević Z, Kuzmanović S, Starović M, Stevanović M, Delibašić G, Živković S. Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina. 2019;34(2):103-109..
Aleksić, Goran, Milićević, Zoran, Kuzmanović, Slobodan, Starović, Mira, Stevanović, Miloš, Delibašić, Goran, Živković, Svetlana, "Efficacy of copper citrate in grapevine disease control" in Pesticidi i fitomedicina, 34, no. 2 (2019):103-109.

Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides

Živković, Svetlana; Stevanović, Miloš; Đurović, Sanja; Ristić, Danijela; Stošić, Stefan

(Institute of Pesticides and Environmental Protection, 2018)

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 
@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}
Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z
Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .
Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .
6

Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi

Zivkovic, Svetlana; Stošić, Stefan; Stevanović, Miloš; Gašić, Katarina; Aleksic, Goran; Vučurović, Ivan; Ristić, Danijela

(Herbološko društvo Srbije, 2017)

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Gašić, Katarina
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Ristić, Danijela
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1013
AB  - Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.
PB  - Herbološko društvo Srbije
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi
EP  - 343
SP  - 331
VL  - 133
DO  - 10.2298/ZMSPN1733331Z
ER  - 
@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Stevanović, Miloš and Gašić, Katarina and Aleksic, Goran and Vučurović, Ivan and Ristić, Danijela",
year = "2017",
abstract = "Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.",
publisher = "Herbološko društvo Srbije",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi",
pages = "343-331",
volume = "133",
doi = "10.2298/ZMSPN1733331Z"
}
Zivkovic, S., Stošić, S., Stevanović, M., Gašić, K., Aleksic, G., Vučurović, I.,& Ristić, D.. (2017). Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke
Herbološko društvo Srbije., 133, 331-343.
https://doi.org/10.2298/ZMSPN1733331Z
Zivkovic S, Stošić S, Stevanović M, Gašić K, Aleksic G, Vučurović I, Ristić D. Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke. 2017;133:331-343.
doi:10.2298/ZMSPN1733331Z .
Zivkovic, Svetlana, Stošić, Stefan, Stevanović, Miloš, Gašić, Katarina, Aleksic, Goran, Vučurović, Ivan, Ristić, Danijela, "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi" in Zbornik Matice srpske za prirodne nauke, 133 (2017):331-343,
https://doi.org/10.2298/ZMSPN1733331Z . .
1

Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia

Pavlović, Snežana; Ristić, Danijela; Vučurović, Ivan; Stevanović, Miloš; Stojanović, Sasa; Kuzmanović, Slobodan; Starović, Mira

(Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca, 2016)

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
AU  - Stojanović, Sasa
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/450
AB  - Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.
PB  - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia
EP  - 417
IS  - 2
SP  - 411
VL  - 44
DO  - 10.15835/nbha44210488
ER  - 
@article{
author = "Pavlović, Snežana and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš and Stojanović, Sasa and Kuzmanović, Slobodan and Starović, Mira",
year = "2016",
abstract = "Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.",
publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia",
pages = "417-411",
number = "2",
volume = "44",
doi = "10.15835/nbha44210488"
}
Pavlović, S., Ristić, D., Vučurović, I., Stevanović, M., Stojanović, S., Kuzmanović, S.,& Starović, M.. (2016). Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 44(2), 411-417.
https://doi.org/10.15835/nbha44210488
Pavlović S, Ristić D, Vučurović I, Stevanović M, Stojanović S, Kuzmanović S, Starović M. Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2016;44(2):411-417.
doi:10.15835/nbha44210488 .
Pavlović, Snežana, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, Stojanović, Sasa, Kuzmanović, Slobodan, Starović, Mira, "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44, no. 2 (2016):411-417,
https://doi.org/10.15835/nbha44210488 . .
13
2
7

Antifungal activities of different essential oils against anise seeds mycopopulations

Starović, Mira; Ristić, Danijela; Pavlović, Snežana; Ristić, Mihailo; Stevanović, Miloš; AlJuhaimi, Fahad; Naydun, Svetlana; Ozcan, Mehmet Musa

(M H Schaper Gmbh Co Kg, Alfeld, 2016)

TY  - JOUR
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - Ristić, Mihailo
AU  - Stevanović, Miloš
AU  - AlJuhaimi, Fahad
AU  - Naydun, Svetlana
AU  - Ozcan, Mehmet Musa
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/441
AB  - The aim of this study was to investigate the possibility of biological control of fungal species isolated from anise seeds using essential oils from medicinal plants: mint (Mentha spicata L.), sage (Salvia fruticosa L.), rosemary (Rosmarinus officinalis L.), anise (Pimpinella anisum L.), bitter fennel (Foeniculum vulgare spp. piperituum L.) and myrtle (Myrtus communis L.). Ten fungal species isolated from anise seeds: Bipolaris/Drechslera sorociniana, Fusarium subglutinans, F. vertricilioides, F. oxysporum, F. tricinctum, F sporotrichioides, F. equiseti, F. incarnatum, F proliferatum and Macrophomina phaseolina, were used in this experiment The minimum inhibitory concentrations (MIC) were determined by micro-dilution method using selected essential oils (EOs). A qualitative and quantitative chemical analyses of EOs were carried out. All EOs exhibited a significant antifungal activity against all tested fungal isolates. The myrtle EO proved to be the most potent one (MIC 0.0003-3.25 mg/mL, then mint 0.0003-7.75 mg/mL and sage 0.0003-10 mg/mL). All tested fungi were observed to have a susceptibility to all selected essential oils. These results suggest the possibility for application of the EOs in biological control of anise production.
PB  - M H Schaper Gmbh Co Kg, Alfeld
T2  - Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene
T1  - Antifungal activities of different essential oils against anise seeds mycopopulations
EP  - 78
IS  - 3
SP  - 72
VL  - 67
DO  - 10.2376/0003-925X-67-72
ER  - 
@article{
author = "Starović, Mira and Ristić, Danijela and Pavlović, Snežana and Ristić, Mihailo and Stevanović, Miloš and AlJuhaimi, Fahad and Naydun, Svetlana and Ozcan, Mehmet Musa",
year = "2016",
abstract = "The aim of this study was to investigate the possibility of biological control of fungal species isolated from anise seeds using essential oils from medicinal plants: mint (Mentha spicata L.), sage (Salvia fruticosa L.), rosemary (Rosmarinus officinalis L.), anise (Pimpinella anisum L.), bitter fennel (Foeniculum vulgare spp. piperituum L.) and myrtle (Myrtus communis L.). Ten fungal species isolated from anise seeds: Bipolaris/Drechslera sorociniana, Fusarium subglutinans, F. vertricilioides, F. oxysporum, F. tricinctum, F sporotrichioides, F. equiseti, F. incarnatum, F proliferatum and Macrophomina phaseolina, were used in this experiment The minimum inhibitory concentrations (MIC) were determined by micro-dilution method using selected essential oils (EOs). A qualitative and quantitative chemical analyses of EOs were carried out. All EOs exhibited a significant antifungal activity against all tested fungal isolates. The myrtle EO proved to be the most potent one (MIC 0.0003-3.25 mg/mL, then mint 0.0003-7.75 mg/mL and sage 0.0003-10 mg/mL). All tested fungi were observed to have a susceptibility to all selected essential oils. These results suggest the possibility for application of the EOs in biological control of anise production.",
publisher = "M H Schaper Gmbh Co Kg, Alfeld",
journal = "Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene",
title = "Antifungal activities of different essential oils against anise seeds mycopopulations",
pages = "78-72",
number = "3",
volume = "67",
doi = "10.2376/0003-925X-67-72"
}
Starović, M., Ristić, D., Pavlović, S., Ristić, M., Stevanović, M., AlJuhaimi, F., Naydun, S.,& Ozcan, M. M.. (2016). Antifungal activities of different essential oils against anise seeds mycopopulations. in Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene
M H Schaper Gmbh Co Kg, Alfeld., 67(3), 72-78.
https://doi.org/10.2376/0003-925X-67-72
Starović M, Ristić D, Pavlović S, Ristić M, Stevanović M, AlJuhaimi F, Naydun S, Ozcan MM. Antifungal activities of different essential oils against anise seeds mycopopulations. in Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene. 2016;67(3):72-78.
doi:10.2376/0003-925X-67-72 .
Starović, Mira, Ristić, Danijela, Pavlović, Snežana, Ristić, Mihailo, Stevanović, Miloš, AlJuhaimi, Fahad, Naydun, Svetlana, Ozcan, Mehmet Musa, "Antifungal activities of different essential oils against anise seeds mycopopulations" in Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene, 67, no. 3 (2016):72-78,
https://doi.org/10.2376/0003-925X-67-72 . .
9
11

Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis

Gavrilović, Veljko; Stošić, Stefan; Stevanović, Miloš

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/309
AB  - Severe symptoms of necrosis sweet cherry fruits was recorded during 2014. in region of Šabac. About 30 % of fruits (cv. Summit) were affected and necrosis cover 1/3 of fruit surface in average. Gram negative, fluorescent, oxidative bacterial strains were isolated from diseased tissues. Symptoms similar to those, observed in natural infection were recorded on artificial inoculated cherry fruits (cv. Summit and cv. Summbarst). Isolated strains were HR positive, oxidase, pectinase, arginin dehidrolase negative and levan positive (LOPAT +---+). Same characteristics also own check strains Pseudomonas syringae (CFBP 11). According obtained results it was concluded that necrosis of sweet cherry fruits is caused by Pseudomonas syringae. Further characteristic in order proper detection of pathogens, including molecular methods are underway.
AB  - U radu su prikazani rezultati proučavanja sojeva bakterija izolovanih iz nekrotičnih plodova trešnje sorte Samit (područje Šapca). Nekroza zahvata u proseku oko 1/3 ploda koji potpuno gubi tržišnu vrednost. Bolešću biva zahvaćeno oko 30% plodova. Primenom standardnih bakterioloških metoda (izolacijom na hranljivim podlogama), proverom patogenosti i proučavanjem bakterioloških karakteristika, zaključeno je da pomenute simptome prouzrokuje fitopatogena bakterija Pseudomonas syringae, široko rasprostranjen patogen naročito koštičavih voćaka.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis
T1  - Pseudomonas syringae - prouzrokovač nekroze plodova trešnje
EP  - 180
IS  - 4
SP  - 176
VL  - 65
DO  - 10.5937/zasbilj1404176G
ER  - 
@article{
author = "Gavrilović, Veljko and Stošić, Stefan and Stevanović, Miloš",
year = "2014",
abstract = "Severe symptoms of necrosis sweet cherry fruits was recorded during 2014. in region of Šabac. About 30 % of fruits (cv. Summit) were affected and necrosis cover 1/3 of fruit surface in average. Gram negative, fluorescent, oxidative bacterial strains were isolated from diseased tissues. Symptoms similar to those, observed in natural infection were recorded on artificial inoculated cherry fruits (cv. Summit and cv. Summbarst). Isolated strains were HR positive, oxidase, pectinase, arginin dehidrolase negative and levan positive (LOPAT +---+). Same characteristics also own check strains Pseudomonas syringae (CFBP 11). According obtained results it was concluded that necrosis of sweet cherry fruits is caused by Pseudomonas syringae. Further characteristic in order proper detection of pathogens, including molecular methods are underway., U radu su prikazani rezultati proučavanja sojeva bakterija izolovanih iz nekrotičnih plodova trešnje sorte Samit (područje Šapca). Nekroza zahvata u proseku oko 1/3 ploda koji potpuno gubi tržišnu vrednost. Bolešću biva zahvaćeno oko 30% plodova. Primenom standardnih bakterioloških metoda (izolacijom na hranljivim podlogama), proverom patogenosti i proučavanjem bakterioloških karakteristika, zaključeno je da pomenute simptome prouzrokuje fitopatogena bakterija Pseudomonas syringae, široko rasprostranjen patogen naročito koštičavih voćaka.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis, Pseudomonas syringae - prouzrokovač nekroze plodova trešnje",
pages = "180-176",
number = "4",
volume = "65",
doi = "10.5937/zasbilj1404176G"
}
Gavrilović, V., Stošić, S.,& Stevanović, M.. (2014). Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(4), 176-180.
https://doi.org/10.5937/zasbilj1404176G
Gavrilović V, Stošić S, Stevanović M. Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis. in Zaštita bilja. 2014;65(4):176-180.
doi:10.5937/zasbilj1404176G .
Gavrilović, Veljko, Stošić, Stefan, Stevanović, Miloš, "Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis" in Zaštita bilja, 65, no. 4 (2014):176-180,
https://doi.org/10.5937/zasbilj1404176G . .

Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method

Gavrilović, Veljko; Stanisavljević, Rade; Stošić, Stefan; Stevanović, Miloš; Aleksić, Goran; Stajić, Milica; Dolovac, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stanisavljević, Rade
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Aleksić, Goran
AU  - Stajić, Milica
AU  - Dolovac, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/301
AB  - Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria.
AB  - U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method
T1  - Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova
EP  - 123
IS  - 3
SP  - 117
VL  - 65
DO  - 10.5937/zasbilj1403117G
ER  - 
@article{
author = "Gavrilović, Veljko and Stanisavljević, Rade and Stošić, Stefan and Stevanović, Miloš and Aleksić, Goran and Stajić, Milica and Dolovac, Nenad",
year = "2014",
abstract = "Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria., U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method, Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova",
pages = "123-117",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403117G"
}
Gavrilović, V., Stanisavljević, R., Stošić, S., Stevanović, M., Aleksić, G., Stajić, M.,& Dolovac, N.. (2014). Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 117-123.
https://doi.org/10.5937/zasbilj1403117G
Gavrilović V, Stanisavljević R, Stošić S, Stevanović M, Aleksić G, Stajić M, Dolovac N. Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja. 2014;65(3):117-123.
doi:10.5937/zasbilj1403117G .
Gavrilović, Veljko, Stanisavljević, Rade, Stošić, Stefan, Stevanović, Miloš, Aleksić, Goran, Stajić, Milica, Dolovac, Nenad, "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method" in Zaštita bilja, 65, no. 3 (2014):117-123,
https://doi.org/10.5937/zasbilj1403117G . .

Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion

Stevanović, Miloš; Dolovac, Nenad; Trkulja, Nenad; Milosavljević, Anja; Kuzmanović, Slobodan; Aleksić, Goran

(Institute for Plant Protection and Environment, Belgrade, 2014)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Kuzmanović, Slobodan
AU  - Aleksić, Goran
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/328
AB  - Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%).
AB  - Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion
T1  - Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije
EP  - 32
IS  - 1
SP  - 27
VL  - 65
DO  - 10.5937/zasbilj1401027S
ER  - 
@article{
author = "Stevanović, Miloš and Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Kuzmanović, Slobodan and Aleksić, Goran",
year = "2014",
abstract = "Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%)., Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion, Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije",
pages = "32-27",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401027S"
}
Stevanović, M., Dolovac, N., Trkulja, N., Milosavljević, A., Kuzmanović, S.,& Aleksić, G.. (2014). Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 27-32.
https://doi.org/10.5937/zasbilj1401027S
Stevanović M, Dolovac N, Trkulja N, Milosavljević A, Kuzmanović S, Aleksić G. Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja. 2014;65(1):27-32.
doi:10.5937/zasbilj1401027S .
Stevanović, Miloš, Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Kuzmanović, Slobodan, Aleksić, Goran, "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion" in Zaštita bilja, 65, no. 1 (2014):27-32,
https://doi.org/10.5937/zasbilj1401027S . .
1

First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia

Stevanović, Miloš; Stanković, Ivana; Vučurović, Ana; Dolovac, Nenad; Pfaf-Dolovac, Erika; Krstić, Branka; Bulajić, Aleksandra

(Amer Phytopathological Soc, St Paul, 2012)

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Dolovac, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/216
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia
EP  - 912
IS  - 6
SP  - 912
VL  - 96
DO  - 10.1094/PDIS-02-12-0179-PDN
ER  - 
@article{
author = "Stevanović, Miloš and Stanković, Ivana and Vučurović, Ana and Dolovac, Nenad and Pfaf-Dolovac, Erika and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia",
pages = "912-912",
number = "6",
volume = "96",
doi = "10.1094/PDIS-02-12-0179-PDN"
}
Stevanović, M., Stanković, I., Vučurović, A., Dolovac, N., Pfaf-Dolovac, E., Krstić, B.,& Bulajić, A.. (2012). First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(6), 912-912.
https://doi.org/10.1094/PDIS-02-12-0179-PDN
Stevanović M, Stanković I, Vučurović A, Dolovac N, Pfaf-Dolovac E, Krstić B, Bulajić A. First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease. 2012;96(6):912-912.
doi:10.1094/PDIS-02-12-0179-PDN .
Stevanović, Miloš, Stanković, Ivana, Vučurović, Ana, Dolovac, Nenad, Pfaf-Dolovac, Erika, Krstić, Branka, Bulajić, Aleksandra, "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia" in Plant Disease, 96, no. 6 (2012):912-912,
https://doi.org/10.1094/PDIS-02-12-0179-PDN . .
4

Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits

Milosavljević, Anja; Stevanović, Miloš; Popović, Tatjana; Đukanović, Lana; Živković, Svetlana; Mitrović, Milana; Trkulja, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2012)

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Stevanović, Miloš
AU  - Popović, Tatjana
AU  - Đukanović, Lana
AU  - Živković, Svetlana
AU  - Mitrović, Milana
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/240
AB  - Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium.
AB  - Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits
T1  - Morfološke i odgajivačke karakteristike izolata Monilinia laxa sa koštičavih voćaka
EP  - 158
IS  - 3
SP  - 148
VL  - 63
ER  - 
@article{
author = "Milosavljević, Anja and Stevanović, Miloš and Popović, Tatjana and Đukanović, Lana and Živković, Svetlana and Mitrović, Milana and Trkulja, Nenad",
year = "2012",
abstract = "Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium., Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits, Morfološke i odgajivačke karakteristike izolata Monilinia laxa sa koštičavih voćaka",
pages = "158-148",
number = "3",
volume = "63"
}
Milosavljević, A., Stevanović, M., Popović, T., Đukanović, L., Živković, S., Mitrović, M.,& Trkulja, N.. (2012). Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 148-158.
Milosavljević A, Stevanović M, Popović T, Đukanović L, Živković S, Mitrović M, Trkulja N. Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits. in Zaštita bilja. 2012;63(3):148-158..
Milosavljević, Anja, Stevanović, Miloš, Popović, Tatjana, Đukanović, Lana, Živković, Svetlana, Mitrović, Milana, Trkulja, Nenad, "Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits" in Zaštita bilja, 63, no. 3 (2012):148-158.

Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides

Trkulja, Nenad; Dolovac, Nenad; Pfaf-Dolovac, Erika; Stevanović, Miloš; Ivanović, Žarko; Štrbanović, Ratibor; Živković, Svetlana

(Institute for Plant Protection and Environment, Belgrade, 2011)

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Stevanović, Miloš
AU  - Ivanović, Žarko
AU  - Štrbanović, Ratibor
AU  - Živković, Svetlana
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/148
AB  - Cercospora leaf spot is the most important disease that occurs on sugar beet in the world. Damages caused by this disease can reduce yields up to 50 %. Resistance to the group of benzimidazole fungicide was determined in 1976. Last twenty years their use has been reduced but they're still used, mainly formulated in mixture with fungicides - sterol demethylation inhibitors (DMI). Sterol demethylation inhibitors (DMI) have been used extensively in the last two decades to control C. beticola in the world, and resistance to them was observed in 2000. The results showed that populations of C. beticola resistant to benzimidazole are present in sugar beet fields and are the dominant population, with the prevalence of resistance 83.87 - 94.28 %. The frequency of resistance to DMI fungicides is smaller, but highly significant and amounts to 12.90 - 14.29 %.
AB  - Pegavost lišća šećerne repe je najznačajnija bolest koja se javlja na šećernoj repi u svetu. Štete koje nastaju usled pojave bolesti mogu smanjiti prinos i do 50%. Prema fungicidima iz grupe benzimidazola rezistentnost je utvrđena 1976. godine. Poslednjih dvadeset godina njihova upotreba je redukovana, ali se i dalje koriste uglavnom formulisani u mešavini sa fungicidima inhibitorima sinteze sterola (DMI). Fungicidi inhibitori demetilacije sterola (DMI), se zadnje dve decenije intenzivno koriste za suzbijanje C. beticola u svetu, a pojava rezistentnosti prema njima konstatovana je 2000. godine. Rezultati istraživanja su pokazali da su populacije C. beticola rezistentne prema benzimidazolima prisutne na poljima šećerne repe i čine dominantnu populaciju uz učestalost rezistentnosti 83.87 - 94.28 %. Učestalost rezistentnosti prema DMI fungicidima je manja, ali veoma značajna i iznosi 12.90 - 14.29 %.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides
T1  - Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima
EP  - 117
IS  - 2
SP  - 109
VL  - 62
ER  - 
@article{
author = "Trkulja, Nenad and Dolovac, Nenad and Pfaf-Dolovac, Erika and Stevanović, Miloš and Ivanović, Žarko and Štrbanović, Ratibor and Živković, Svetlana",
year = "2011",
abstract = "Cercospora leaf spot is the most important disease that occurs on sugar beet in the world. Damages caused by this disease can reduce yields up to 50 %. Resistance to the group of benzimidazole fungicide was determined in 1976. Last twenty years their use has been reduced but they're still used, mainly formulated in mixture with fungicides - sterol demethylation inhibitors (DMI). Sterol demethylation inhibitors (DMI) have been used extensively in the last two decades to control C. beticola in the world, and resistance to them was observed in 2000. The results showed that populations of C. beticola resistant to benzimidazole are present in sugar beet fields and are the dominant population, with the prevalence of resistance 83.87 - 94.28 %. The frequency of resistance to DMI fungicides is smaller, but highly significant and amounts to 12.90 - 14.29 %., Pegavost lišća šećerne repe je najznačajnija bolest koja se javlja na šećernoj repi u svetu. Štete koje nastaju usled pojave bolesti mogu smanjiti prinos i do 50%. Prema fungicidima iz grupe benzimidazola rezistentnost je utvrđena 1976. godine. Poslednjih dvadeset godina njihova upotreba je redukovana, ali se i dalje koriste uglavnom formulisani u mešavini sa fungicidima inhibitorima sinteze sterola (DMI). Fungicidi inhibitori demetilacije sterola (DMI), se zadnje dve decenije intenzivno koriste za suzbijanje C. beticola u svetu, a pojava rezistentnosti prema njima konstatovana je 2000. godine. Rezultati istraživanja su pokazali da su populacije C. beticola rezistentne prema benzimidazolima prisutne na poljima šećerne repe i čine dominantnu populaciju uz učestalost rezistentnosti 83.87 - 94.28 %. Učestalost rezistentnosti prema DMI fungicidima je manja, ali veoma značajna i iznosi 12.90 - 14.29 %.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides, Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima",
pages = "117-109",
number = "2",
volume = "62"
}
Trkulja, N., Dolovac, N., Pfaf-Dolovac, E., Stevanović, M., Ivanović, Ž., Štrbanović, R.,& Živković, S.. (2011). Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 62(2), 109-117.
Trkulja N, Dolovac N, Pfaf-Dolovac E, Stevanović M, Ivanović Ž, Štrbanović R, Živković S. Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides. in Zaštita bilja. 2011;62(2):109-117..
Trkulja, Nenad, Dolovac, Nenad, Pfaf-Dolovac, Erika, Stevanović, Miloš, Ivanović, Žarko, Štrbanović, Ratibor, Živković, Svetlana, "Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides" in Zaštita bilja, 62, no. 2 (2011):109-117.

The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia

Dolovac, Nenad; Trkulja, Nenad; Aleksić, Goran; Stevanović, Miloš; Pfaf-Dolovac, Erika; Popović, Tatjana; Ivanović, Žarko

(Institute for Plant Protection and Environment, Belgrade, 2011)

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Aleksić, Goran
AU  - Stevanović, Miloš
AU  - Pfaf-Dolovac, Erika
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/185
AB  - The causal agent of peach leaf curl Taphrina deformans, in recent years in Serbia occurs regularly and causes significant economic losses. As the leaf curl important in many areas of growing peaches in the world, great attention is focused on finding a measure of control. According to recommendations from the literature sources of chemical protection against this pathogen should be carried out in several treatments, ie. in the fall after the leaves fall and the spring just before and during the swelling buds. As is well known that the pathogen T. deformans infection achieved solely through the undifferentiated tissue swelling in the bud stage of development, there were determined the effectiveness of fungicides recommended in all phenological stages of development. The study was conducted during fall 2006. and spring of 2007. year, the Summerset peach orchards cultivars in conditions of natural infection at the site of Bela Crkva. We tested seven different varieties, which are comprised of individual and combined treatments, so that the period covered by the three defined phenological stages of peach, starting from autumn treatment stage of development in 50% of leaves yellow and dorman and growth stages of vegetation where the applied fungicide copper hidroksid, and phenophase bud swell to when fungicide was applied ziram. In conditions of high levels of natural infection in the control plots, the highest efficiency demonstrated the variant that contained the treatment of swelling in the bud stage of development (96.22 - 97.84%), while other variants did not show satisfactory efficiency (7.78 - 16.85% ). These results significantly contribute to an improved method of groweing peach with substantial savings on chemicals without losses caused by disease, peach leaf curl.
AB  - Prouzrokovač kovrdžavosti lista breskve Taphrina deformans, poslednjih godina u Srbiji redovno se javlja i nanosi značajne ekonomske gubitke. Kako je kovrdžavost lista značajna u mnogim područjima gajenja breskve u svetu velika pažnja usmerena je na iznalaženje mera kontrole. Prema preporukama iz literaturnih izvora hemijsku zaštitu protiv ovog patogena treba sprovoditi u nekoliko tretmana, tj. u jesen nakon opadanja lišća i u proleće neposredno pre i u toku bubrenja pupoljaka. Kako je poznato da patogen T. deformans ostvaruje infekciju isključivo preko nediferenciranog tkiva u fenofazi bubrenja pupoljka, vršena su ispitivanja efikasnosti primene fungicida u svim preporučenim fenofazama razvoja. Ispitivanje je obavljeno u periodu jesen 2006. i proleće 2007. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je sedam različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od tri definisane fenofaze razvoja breskve, počevši od jesenjeg tretmana u fenofazi 50% lišća žuto i opalo i fenofaze mirovanja vegetacije gde je primenjen fungicid bakar hidroksid do fenofaze bubrenje pupoljka kada je primenjen fungicid ciram. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost ispoljile su varijante koje su sadržale tretman u fenofazi bubrenja pupoljka (96,22 - 97,84%), dok ostale varijante nisu pokazale zadovoljavajuću efikasnost (7,78 - 16,85%). Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve uz značajnu uštedu hemijskih sredstava bez gubitaka izazvanih oboljenjem kovrdžavosti lista breskve.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia
T1  - Efikasnost rokova primene fungicida za suzbijanje Taphrina deformans, prouzrokovača kovrdžavosti lista breskve u Srbiji
EP  - 226
IS  - 4
SP  - 219
VL  - 62
ER  - 
@article{
author = "Dolovac, Nenad and Trkulja, Nenad and Aleksić, Goran and Stevanović, Miloš and Pfaf-Dolovac, Erika and Popović, Tatjana and Ivanović, Žarko",
year = "2011",
abstract = "The causal agent of peach leaf curl Taphrina deformans, in recent years in Serbia occurs regularly and causes significant economic losses. As the leaf curl important in many areas of growing peaches in the world, great attention is focused on finding a measure of control. According to recommendations from the literature sources of chemical protection against this pathogen should be carried out in several treatments, ie. in the fall after the leaves fall and the spring just before and during the swelling buds. As is well known that the pathogen T. deformans infection achieved solely through the undifferentiated tissue swelling in the bud stage of development, there were determined the effectiveness of fungicides recommended in all phenological stages of development. The study was conducted during fall 2006. and spring of 2007. year, the Summerset peach orchards cultivars in conditions of natural infection at the site of Bela Crkva. We tested seven different varieties, which are comprised of individual and combined treatments, so that the period covered by the three defined phenological stages of peach, starting from autumn treatment stage of development in 50% of leaves yellow and dorman and growth stages of vegetation where the applied fungicide copper hidroksid, and phenophase bud swell to when fungicide was applied ziram. In conditions of high levels of natural infection in the control plots, the highest efficiency demonstrated the variant that contained the treatment of swelling in the bud stage of development (96.22 - 97.84%), while other variants did not show satisfactory efficiency (7.78 - 16.85% ). These results significantly contribute to an improved method of groweing peach with substantial savings on chemicals without losses caused by disease, peach leaf curl., Prouzrokovač kovrdžavosti lista breskve Taphrina deformans, poslednjih godina u Srbiji redovno se javlja i nanosi značajne ekonomske gubitke. Kako je kovrdžavost lista značajna u mnogim područjima gajenja breskve u svetu velika pažnja usmerena je na iznalaženje mera kontrole. Prema preporukama iz literaturnih izvora hemijsku zaštitu protiv ovog patogena treba sprovoditi u nekoliko tretmana, tj. u jesen nakon opadanja lišća i u proleće neposredno pre i u toku bubrenja pupoljaka. Kako je poznato da patogen T. deformans ostvaruje infekciju isključivo preko nediferenciranog tkiva u fenofazi bubrenja pupoljka, vršena su ispitivanja efikasnosti primene fungicida u svim preporučenim fenofazama razvoja. Ispitivanje je obavljeno u periodu jesen 2006. i proleće 2007. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je sedam različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od tri definisane fenofaze razvoja breskve, počevši od jesenjeg tretmana u fenofazi 50% lišća žuto i opalo i fenofaze mirovanja vegetacije gde je primenjen fungicid bakar hidroksid do fenofaze bubrenje pupoljka kada je primenjen fungicid ciram. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost ispoljile su varijante koje su sadržale tretman u fenofazi bubrenja pupoljka (96,22 - 97,84%), dok ostale varijante nisu pokazale zadovoljavajuću efikasnost (7,78 - 16,85%). Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve uz značajnu uštedu hemijskih sredstava bez gubitaka izazvanih oboljenjem kovrdžavosti lista breskve.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia, Efikasnost rokova primene fungicida za suzbijanje Taphrina deformans, prouzrokovača kovrdžavosti lista breskve u Srbiji",
pages = "226-219",
number = "4",
volume = "62"
}
Dolovac, N., Trkulja, N., Aleksić, G., Stevanović, M., Pfaf-Dolovac, E., Popović, T.,& Ivanović, Ž.. (2011). The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 62(4), 219-226.
Dolovac N, Trkulja N, Aleksić G, Stevanović M, Pfaf-Dolovac E, Popović T, Ivanović Ž. The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia. in Zaštita bilja. 2011;62(4):219-226..
Dolovac, Nenad, Trkulja, Nenad, Aleksić, Goran, Stevanović, Miloš, Pfaf-Dolovac, Erika, Popović, Tatjana, Ivanović, Žarko, "The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia" in Zaštita bilja, 62, no. 4 (2011):219-226.

Identification and characterization of peach pathogen Pseudomonas syringae

Gavrilović, Veljko; Dolovac, Nenad; Trkulja, Nenad; Stevanović, Miloš; Živković, Svetlana; Poštić, Dobrivoj; Ivanović, Žarko

(Institute for Plant Protection and Environment, Belgrade, 2011)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Stevanović, Miloš
AU  - Živković, Svetlana
AU  - Poštić, Dobrivoj
AU  - Ivanović, Žarko
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/155
AB  - Pseudomonas syringae is economically important plant pathogen, found on a number of hosts including fruit trees, field crops, vegetables and decorative plants. This phytopathogenic bacteria is becoming a quite widespread pathogen on the fruit trees in Serbia, causing significant economic loses. Up to now it was experimentally confirmed as a pathogen on the pear, apple, apricot, cherry, sour cherry, plum trees as well as raspberries. In this study Pseudomonas syringae was identificated as pathogen on peach tree in Serbia. Detection of syrB gene was used for identification phytotoxins tipical for P. syringae pv. syringae. Our experiment showed that detection of syrB genes can be used as a diagnostic tool in determining pathovars of Pseudomonas syringae parasites on peach trees.
AB  - Pseudomonas syringae ekonomski značajan biljni patogen širokog kruga domaćina koji uključuje voćke, ratarske, povrtarske i ukrasne biljke. Ova fitopatogena bakterija naročitu pažnju privlači kao patogen voćaka prouzrokujući sve veće štete. Do sada je eksperimentalno potvrđen kao parazit kruške, jabuke, kajsije, trešnje, višnje, šljive i maline. Pseudomonas syringae je identifikovan kao patogen breskve u Srbiji. Detekcija syrB gen je korišćena za identifikaciju fitotoksina karakterističnog za P. syringae pv. syringae. Naš je eksperiment pokazao da se detekcijom SyrB gena kao dijagnostičkog alata može identifikovati patogeni varijetet patogen breskve.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Identification and characterization of peach pathogen Pseudomonas syringae
T1  - Identifikacija i karakterizacija bakterije Pseudomonas syringae patogena breskve
EP  - 38
IS  - 1
SP  - 25
VL  - 62
ER  - 
@article{
author = "Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Stevanović, Miloš and Živković, Svetlana and Poštić, Dobrivoj and Ivanović, Žarko",
year = "2011",
abstract = "Pseudomonas syringae is economically important plant pathogen, found on a number of hosts including fruit trees, field crops, vegetables and decorative plants. This phytopathogenic bacteria is becoming a quite widespread pathogen on the fruit trees in Serbia, causing significant economic loses. Up to now it was experimentally confirmed as a pathogen on the pear, apple, apricot, cherry, sour cherry, plum trees as well as raspberries. In this study Pseudomonas syringae was identificated as pathogen on peach tree in Serbia. Detection of syrB gene was used for identification phytotoxins tipical for P. syringae pv. syringae. Our experiment showed that detection of syrB genes can be used as a diagnostic tool in determining pathovars of Pseudomonas syringae parasites on peach trees., Pseudomonas syringae ekonomski značajan biljni patogen širokog kruga domaćina koji uključuje voćke, ratarske, povrtarske i ukrasne biljke. Ova fitopatogena bakterija naročitu pažnju privlači kao patogen voćaka prouzrokujući sve veće štete. Do sada je eksperimentalno potvrđen kao parazit kruške, jabuke, kajsije, trešnje, višnje, šljive i maline. Pseudomonas syringae je identifikovan kao patogen breskve u Srbiji. Detekcija syrB gen je korišćena za identifikaciju fitotoksina karakterističnog za P. syringae pv. syringae. Naš je eksperiment pokazao da se detekcijom SyrB gena kao dijagnostičkog alata može identifikovati patogeni varijetet patogen breskve.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Identification and characterization of peach pathogen Pseudomonas syringae, Identifikacija i karakterizacija bakterije Pseudomonas syringae patogena breskve",
pages = "38-25",
number = "1",
volume = "62"
}
Gavrilović, V., Dolovac, N., Trkulja, N., Stevanović, M., Živković, S., Poštić, D.,& Ivanović, Ž.. (2011). Identification and characterization of peach pathogen Pseudomonas syringae. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 62(1), 25-38.
Gavrilović V, Dolovac N, Trkulja N, Stevanović M, Živković S, Poštić D, Ivanović Ž. Identification and characterization of peach pathogen Pseudomonas syringae. in Zaštita bilja. 2011;62(1):25-38..
Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Stevanović, Miloš, Živković, Svetlana, Poštić, Dobrivoj, Ivanović, Žarko, "Identification and characterization of peach pathogen Pseudomonas syringae" in Zaštita bilja, 62, no. 1 (2011):25-38.

Etiological study of sour cherry fruits bacterial necrosis in south Banat

Gavrilović, Veljko; Ivanović, Žarko; Živković, Svetlana; Poštić, Dobrivoj; Stevanović, Miloš; Trkulja, Nenad

(Institute for Plant Protection and Environment, Belgrade, 2011)

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Živković, Svetlana
AU  - Poštić, Dobrivoj
AU  - Stevanović, Miloš
AU  - Trkulja, Nenad
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/150
AB  - Characteristics of pathogenic Pseudomonas bacterial strains isolated from necrotic sour cherry fruits (Kelleris 14) in region of south Banat. First symptoms of disease appeared as dark green wet spot. Later on it turns to brown or black sunken necrosis. During rainy spring percent of affected fruits reached up 60-80. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Investigated strains cause severe necrosis of artificially sour cherry fruits (Kelleris 14) as well as necroses on artificially inoculated cherry, pear and lemon fruits, lilac leaves and bean pods. Positive results were recorded in gelatin and aesculin hydrolysis tests while, tyrosinase and tartrate tests were negative (typical characteristics of P.s. pv.syringae). According the obtained results it was concluded that severe necrosis of sour cherry fruits (Kelleris 14) in South Banat is caused by Pseudomonas syringae pv syringae.
AB  - U radu su saopštene karakteristike izolata bakterije Pseudomonas syringae izolovane iz nekrozom zahvaćenih plodova višnje (Kelleris 14) na području Južnog Banata. Simptomi sa karakterističnim crnomrkim ulegnutim nekrozama prikupljani su u periodu 2008-2010 godine. Iz obolelog tkiva izolavani su gramnegativni, fluorescentni sojevi bakterija, koji glukozu metabolišu isključivo u aerobnim uslovima (oksidativno); prouzrokuju HR duvana i stvaraju levan, ali ne proizvode oksidazu, pektinazu i arginindehidrolazu (LOPAT I). Proučeni izolati prouzrokuju nekrozu plodova višnje, trešnje, kruške i limuna, listova jorgovana i mahuna boranije. U pogledu diferencijalnih testova za patogene varijetete P. syringae, rezultati ukazuju da dobijeni sojevi hidrolizuju želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Na osnovu dobijenih rezultata i odlika izolovanih sojeva zaključeno je, da nekrozu plodova višnje veoma visokog intenziteta, zapaženu poslednjih godina u Južnom Banatu, prouzrokuje bakterija Pseudomonas syringae pv. syringae.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Etiological study of sour cherry fruits bacterial necrosis in south Banat
T1  - Etiološka proučavanja bakteriozne pegavosti plodova višnje na području južnog Banata
EP  - 128
IS  - 2
SP  - 119
VL  - 62
ER  - 
@article{
author = "Gavrilović, Veljko and Ivanović, Žarko and Živković, Svetlana and Poštić, Dobrivoj and Stevanović, Miloš and Trkulja, Nenad",
year = "2011",
abstract = "Characteristics of pathogenic Pseudomonas bacterial strains isolated from necrotic sour cherry fruits (Kelleris 14) in region of south Banat. First symptoms of disease appeared as dark green wet spot. Later on it turns to brown or black sunken necrosis. During rainy spring percent of affected fruits reached up 60-80. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Investigated strains cause severe necrosis of artificially sour cherry fruits (Kelleris 14) as well as necroses on artificially inoculated cherry, pear and lemon fruits, lilac leaves and bean pods. Positive results were recorded in gelatin and aesculin hydrolysis tests while, tyrosinase and tartrate tests were negative (typical characteristics of P.s. pv.syringae). According the obtained results it was concluded that severe necrosis of sour cherry fruits (Kelleris 14) in South Banat is caused by Pseudomonas syringae pv syringae., U radu su saopštene karakteristike izolata bakterije Pseudomonas syringae izolovane iz nekrozom zahvaćenih plodova višnje (Kelleris 14) na području Južnog Banata. Simptomi sa karakterističnim crnomrkim ulegnutim nekrozama prikupljani su u periodu 2008-2010 godine. Iz obolelog tkiva izolavani su gramnegativni, fluorescentni sojevi bakterija, koji glukozu metabolišu isključivo u aerobnim uslovima (oksidativno); prouzrokuju HR duvana i stvaraju levan, ali ne proizvode oksidazu, pektinazu i arginindehidrolazu (LOPAT I). Proučeni izolati prouzrokuju nekrozu plodova višnje, trešnje, kruške i limuna, listova jorgovana i mahuna boranije. U pogledu diferencijalnih testova za patogene varijetete P. syringae, rezultati ukazuju da dobijeni sojevi hidrolizuju želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Na osnovu dobijenih rezultata i odlika izolovanih sojeva zaključeno je, da nekrozu plodova višnje veoma visokog intenziteta, zapaženu poslednjih godina u Južnom Banatu, prouzrokuje bakterija Pseudomonas syringae pv. syringae.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Etiological study of sour cherry fruits bacterial necrosis in south Banat, Etiološka proučavanja bakteriozne pegavosti plodova višnje na području južnog Banata",
pages = "128-119",
number = "2",
volume = "62"
}
Gavrilović, V., Ivanović, Ž., Živković, S., Poštić, D., Stevanović, M.,& Trkulja, N.. (2011). Etiological study of sour cherry fruits bacterial necrosis in south Banat. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 62(2), 119-128.
Gavrilović V, Ivanović Ž, Živković S, Poštić D, Stevanović M, Trkulja N. Etiological study of sour cherry fruits bacterial necrosis in south Banat. in Zaštita bilja. 2011;62(2):119-128..
Gavrilović, Veljko, Ivanović, Žarko, Živković, Svetlana, Poštić, Dobrivoj, Stevanović, Miloš, Trkulja, Nenad, "Etiological study of sour cherry fruits bacterial necrosis in south Banat" in Zaštita bilja, 62, no. 2 (2011):119-128.