TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/620
AB  - Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum
EP  - 44
SP  - 29
VL  - 140
DO  - 10.2298/ZMSPN2140029S
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Danijela and Živković, Svetlana",
year = "2021",
abstract = "Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum",
pages = "44-29",
volume = "140",
doi = "10.2298/ZMSPN2140029S"
}

Stošić, S., Ristić, D.,& Živković, S.. (2021). Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 29-44.
https://doi.org/10.2298/ZMSPN2140029S

Stošić S, Ristić D, Živković S. Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke. 2021;140:29-44.
doi:10.2298/ZMSPN2140029S .

Stošić, Stefan, Ristić, Danijela, Živković, Svetlana, "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum" in Zbornik Matice srpske za prirodne nauke, 140 (2021):29-44,
https://doi.org/10.2298/ZMSPN2140029S . .

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 

@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}

Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z

Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .

Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/495
AB  - The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks.
AB  - U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Effects of copper citrate as a defoliant in nursery fruit stock production
T1  - Bakar citrat - defolijant u proizvodnji sadnica voćaka
EP  - 236
IS  - 3-4
SP  - 231
VL  - 32
DO  - 10.2298/PIF1704231G
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksić, Goran and Starović, Mira and Živković, Svetlana and Stošić, Stefan and Kuzmanović, Slobodan",
year = "2017",
abstract = "The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks., U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Effects of copper citrate as a defoliant in nursery fruit stock production, Bakar citrat - defolijant u proizvodnji sadnica voćaka",
pages = "236-231",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704231G"
}

Gavrilović, V., Milićević, Z., Aleksić, G., Starović, M., Živković, S., Stošić, S.,& Kuzmanović, S.. (2017). Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 231-236.
https://doi.org/10.2298/PIF1704231G

Gavrilović V, Milićević Z, Aleksić G, Starović M, Živković S, Stošić S, Kuzmanović S. Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina. 2017;32(3-4):231-236.
doi:10.2298/PIF1704231G .

Gavrilović, Veljko, Milićević, Zoran, Aleksić, Goran, Starović, Mira, Živković, Svetlana, Stošić, Stefan, Kuzmanović, Slobodan, "Effects of copper citrate as a defoliant in nursery fruit stock production" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):231-236,
https://doi.org/10.2298/PIF1704231G . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Stojanović, Saša
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/329
AB  - In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum.
AB  - Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Effect of calcium salts on postharvest fungal pathogens in vitro
T1  - In vitro efekat kalcijumovih soli na gljivične skladišne patogene
EP  - 46
IS  - 1
SP  - 40
VL  - 65
DO  - 10.5937/zasbilj1401040S
ER  - 

@article{
author = "Stošić, Stefan and Stojanović, Saša and Milosavljević, Anja and Pfaf-Dolovac, Erika and Živković, Svetlana",
year = "2014",
abstract = "In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum., Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Effect of calcium salts on postharvest fungal pathogens in vitro, In vitro efekat kalcijumovih soli na gljivične skladišne patogene",
pages = "46-40",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401040S"
}

Stošić, S., Stojanović, S., Milosavljević, A., Pfaf-Dolovac, E.,& Živković, S.. (2014). Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 40-46.
https://doi.org/10.5937/zasbilj1401040S

Stošić S, Stojanović S, Milosavljević A, Pfaf-Dolovac E, Živković S. Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja. 2014;65(1):40-46.
doi:10.5937/zasbilj1401040S .

Stošić, Stefan, Stojanović, Saša, Milosavljević, Anja, Pfaf-Dolovac, Erika, Živković, Svetlana, "Effect of calcium salts on postharvest fungal pathogens in vitro" in Zaštita bilja, 65, no. 1 (2014):40-46,
https://doi.org/10.5937/zasbilj1401040S . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stanisavljević, Rade
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Aleksić, Goran
AU  - Stajić, Milica
AU  - Dolovac, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/301
AB  - Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria.
AB  - U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method
T1  - Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova
EP  - 123
IS  - 3
SP  - 117
VL  - 65
DO  - 10.5937/zasbilj1403117G
ER  - 

@article{
author = "Gavrilović, Veljko and Stanisavljević, Rade and Stošić, Stefan and Stevanović, Miloš and Aleksić, Goran and Stajić, Milica and Dolovac, Nenad",
year = "2014",
abstract = "Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria., U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method, Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova",
pages = "123-117",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403117G"
}

Gavrilović, V., Stanisavljević, R., Stošić, S., Stevanović, M., Aleksić, G., Stajić, M.,& Dolovac, N.. (2014). Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 117-123.
https://doi.org/10.5937/zasbilj1403117G

Gavrilović V, Stanisavljević R, Stošić S, Stevanović M, Aleksić G, Stajić M, Dolovac N. Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja. 2014;65(3):117-123.
doi:10.5937/zasbilj1403117G .

Gavrilović, Veljko, Stanisavljević, Rade, Stošić, Stefan, Stevanović, Miloš, Aleksić, Goran, Stajić, Milica, Dolovac, Nenad, "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method" in Zaštita bilja, 65, no. 3 (2014):117-123,
https://doi.org/10.5937/zasbilj1403117G . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/309
AB  - Severe symptoms of necrosis sweet cherry fruits was recorded during 2014. in region of Šabac. About 30 % of fruits (cv. Summit) were affected and necrosis cover 1/3 of fruit surface in average. Gram negative, fluorescent, oxidative bacterial strains were isolated from diseased tissues. Symptoms similar to those, observed in natural infection were recorded on artificial inoculated cherry fruits (cv. Summit and cv. Summbarst). Isolated strains were HR positive, oxidase, pectinase, arginin dehidrolase negative and levan positive (LOPAT +---+). Same characteristics also own check strains Pseudomonas syringae (CFBP 11). According obtained results it was concluded that necrosis of sweet cherry fruits is caused by Pseudomonas syringae. Further characteristic in order proper detection of pathogens, including molecular methods are underway.
AB  - U radu su prikazani rezultati proučavanja sojeva bakterija izolovanih iz nekrotičnih plodova trešnje sorte Samit (područje Šapca). Nekroza zahvata u proseku oko 1/3 ploda koji potpuno gubi tržišnu vrednost. Bolešću biva zahvaćeno oko 30% plodova. Primenom standardnih bakterioloških metoda (izolacijom na hranljivim podlogama), proverom patogenosti i proučavanjem bakterioloških karakteristika, zaključeno je da pomenute simptome prouzrokuje fitopatogena bakterija Pseudomonas syringae, široko rasprostranjen patogen naročito koštičavih voćaka.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis
T1  - Pseudomonas syringae - prouzrokovač nekroze plodova trešnje
EP  - 180
IS  - 4
SP  - 176
VL  - 65
DO  - 10.5937/zasbilj1404176G
ER  - 

@article{
author = "Gavrilović, Veljko and Stošić, Stefan and Stevanović, Miloš",
year = "2014",
abstract = "Severe symptoms of necrosis sweet cherry fruits was recorded during 2014. in region of Šabac. About 30 % of fruits (cv. Summit) were affected and necrosis cover 1/3 of fruit surface in average. Gram negative, fluorescent, oxidative bacterial strains were isolated from diseased tissues. Symptoms similar to those, observed in natural infection were recorded on artificial inoculated cherry fruits (cv. Summit and cv. Summbarst). Isolated strains were HR positive, oxidase, pectinase, arginin dehidrolase negative and levan positive (LOPAT +---+). Same characteristics also own check strains Pseudomonas syringae (CFBP 11). According obtained results it was concluded that necrosis of sweet cherry fruits is caused by Pseudomonas syringae. Further characteristic in order proper detection of pathogens, including molecular methods are underway., U radu su prikazani rezultati proučavanja sojeva bakterija izolovanih iz nekrotičnih plodova trešnje sorte Samit (područje Šapca). Nekroza zahvata u proseku oko 1/3 ploda koji potpuno gubi tržišnu vrednost. Bolešću biva zahvaćeno oko 30% plodova. Primenom standardnih bakterioloških metoda (izolacijom na hranljivim podlogama), proverom patogenosti i proučavanjem bakterioloških karakteristika, zaključeno je da pomenute simptome prouzrokuje fitopatogena bakterija Pseudomonas syringae, široko rasprostranjen patogen naročito koštičavih voćaka.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis, Pseudomonas syringae - prouzrokovač nekroze plodova trešnje",
pages = "180-176",
number = "4",
volume = "65",
doi = "10.5937/zasbilj1404176G"
}

Gavrilović, V., Stošić, S.,& Stevanović, M.. (2014). Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(4), 176-180.
https://doi.org/10.5937/zasbilj1404176G

Gavrilović V, Stošić S, Stevanović M. Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis. in Zaštita bilja. 2014;65(4):176-180.
doi:10.5937/zasbilj1404176G .

Gavrilović, Veljko, Stošić, Stefan, Stevanović, Miloš, "Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis" in Zaštita bilja, 65, no. 4 (2014):176-180,
https://doi.org/10.5937/zasbilj1404176G . .

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Oro, Violeta
AU  - Pfaf-Dolovac, Erika
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/246
AB  - Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum.
AB  - Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Identification of Colletotrichum acutatum from nectarine fruit
T1  - Identifikacija Colletotrichum acutatum sa ploda nektarine
EP  - 138
IS  - 3
SP  - 130
VL  - 63
ER  - 

@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Oro, Violeta and Pfaf-Dolovac, Erika and Stošić, Stefan and Kuzmanović, Slobodan and Ivanović, Žarko",
year = "2012",
abstract = "Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum., Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Identification of Colletotrichum acutatum from nectarine fruit, Identifikacija Colletotrichum acutatum sa ploda nektarine",
pages = "138-130",
number = "3",
volume = "63"
}

Živković, S., Gavrilović, V., Oro, V., Pfaf-Dolovac, E., Stošić, S., Kuzmanović, S.,& Ivanović, Ž.. (2012). Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 130-138.

Živković S, Gavrilović V, Oro V, Pfaf-Dolovac E, Stošić S, Kuzmanović S, Ivanović Ž. Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja. 2012;63(3):130-138..

Živković, Svetlana, Gavrilović, Veljko, Oro, Violeta, Pfaf-Dolovac, Erika, Stošić, Stefan, Kuzmanović, Slobodan, Ivanović, Žarko, "Identification of Colletotrichum acutatum from nectarine fruit" in Zaštita bilja, 63, no. 3 (2012):130-138.