TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Lela
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/725
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
IS  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Lela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
number = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, L.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12(803789).
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić L, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12(803789).
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Lela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12, no. 803789 (2022),
https://doi.org/10.3389/fmicb.2021.803789 . .

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
American Phytopathological Society., 103(10), 2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Anđelka, Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/905
AB  - Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Differentiation of Pseudomonas syringae pathovars originating from stone fruits
EP  - 229
IS  - 3
SP  - 219
VL  - 27
DO  - 10.2298/PIF1203219G
ER  - 

@article{
author = "Gašić, Katarina and Prokić, Anđelka and Ivanović, Milan and Kuzmanović, Nemanja and Obradović, Aleksa",
year = "2012",
abstract = "Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Differentiation of Pseudomonas syringae pathovars originating from stone fruits",
pages = "229-219",
number = "3",
volume = "27",
doi = "10.2298/PIF1203219G"
}

Gašić, K., Prokić, A., Ivanović, M., Kuzmanović, N.,& Obradović, A.. (2012). Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 27(3), 219-229.
https://doi.org/10.2298/PIF1203219G

Gašić K, Prokić A, Ivanović M, Kuzmanović N, Obradović A. Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina. 2012;27(3):219-229.
doi:10.2298/PIF1203219G .

Gašić, Katarina, Prokić, Anđelka, Ivanović, Milan, Kuzmanović, Nemanja, Obradović, Aleksa, "Differentiation of Pseudomonas syringae pathovars originating from stone fruits" in Pesticidi i fitomedicina, 27, no. 3 (2012):219-229,
https://doi.org/10.2298/PIF1203219G . .

TY  - JOUR
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Šević, Milan
AU  - Pulawska, J.
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/866
AB  - Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.
PB  - Springer
T2  - Journal of Plant Pathology
T1  - Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina
IS  - 1
VL  - 94
DO  - 10.4454/jpp.v94i1sup.020
ER  - 

@article{
author = "Prokić, Anđelka and Gašić, Katarina and Ivanović, Milan and Kuzmanović, Nemanja and Šević, Milan and Pulawska, J. and Obradović, Aleksa",
year = "2012",
abstract = "Timley and reliable detection of the hazelnut pathogen Xanthomonas arboricola pv. corylina (Xac) is essential for the production of good quality disease-free planting material. In order to improve knowledge on diagnostic tools for this quarantine bacterium, the effectiveness of laboratory methods recomended by EPPO and additional modified procedures developed within COST873 was compared. Methods included the classical, biochemical, serological, pathogenicity and molecular approach. To confirm the presence of the pathogen in plant tissue, a combination of different techniques is necessary. For successful isolation of Xac cells from different hazelnut organs, general nutrient agar or semi-selective glucose amended media are recommended. Traditional biochemical techniques are still necessary for the identification of isolates at the species level. ELISA and IF are useful as screening assays for the early and rapid pathogen detection. In addition, PCR-based methods proved to be useful for the rapid detection of Xac cells, although highly pathovar-specific genetic tool is not available. Identification at the pathovar level requires pathogenicity tests on hazelnut plants that enable confirmation of strains' pathogenic nature and reproduction of symptoms. However, a choice of various pathogenicity testing procedures should be made based on testing material available and type and dynamics of symptom development.",
publisher = "Springer",
journal = "Journal of Plant Pathology",
title = "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina",
number = "1",
volume = "94",
doi = "10.4454/jpp.v94i1sup.020"
}

Prokić, A., Gašić, K., Ivanović, M., Kuzmanović, N., Šević, M., Pulawska, J.,& Obradović, A.. (2012). Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina. in Journal of Plant Pathology
Springer., 94(1).
https://doi.org/10.4454/jpp.v94i1sup.020

Prokić A, Gašić K, Ivanović M, Kuzmanović N, Šević M, Pulawska J, Obradović A. Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina. in Journal of Plant Pathology. 2012;94(1).
doi:10.4454/jpp.v94i1sup.020 .

Prokić, Anđelka, Gašić, Katarina, Ivanović, Milan, Kuzmanović, Nemanja, Šević, Milan, Pulawska, J., Obradović, Aleksa, "Detection and identification methods and new tests as developed and used in the framework of COST873 for bacteria pathogenic to stone fruits and nuts: Xanthomonas arboricola pv. corylina" in Journal of Plant Pathology, 94, no. 1 (2012),
https://doi.org/10.4454/jpp.v94i1sup.020 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Kuzmanović, Nemanja
AU  - Ćalić, Anđelka
AU  - Gašić, Katarina
AU  - Ivanović, Milan
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1077
AB  - U voćarstvu naše zemlje koštičave voćke zauzimaju značajno mesto i njihovo gajenje predstavlja profitabilnu delatnost. Međutim, rentabilnost voćarske proizvodnje mogu ugroziti različite biljne bolesti. Po značaju, raširenosti, ali i nemogućnosti uspešne kontrole, izdvajaju se oboljenja bakteriozne i fitoplazmozne prirode. Bakteriozno izumiranje, koje prouzrokuje Pseudomo nas syringae sa svoja dva patogena varijeteta syringae i morsprunorum, zadaje ozbiljne probleme voćarima u našoj zemlji. Značajan prob lem, takođe, predstavlja i Agrobacterium tumefaciens, prouzrokovač bakterioznog raka korena, koji nanosi štete u rasadničarskoj proizvodnji. U radu su pred stavljena i oboljenja koja nisu prisutna u našoj zemlji, a u svetu su opisana kao izuzetno destruktivna. To su bakteriozno izumiranje breskve, koje prouzrokuje P. s. pv. persicae i bakteriozna pegavost lišća koštičavih voćaka, čiji je prouzrokovač Xanthomonas arboricola pv. pruni. U Srbiji je prisutno i fitoplazmozno oboljenje Evropsko žutilo koštičavih voćaka na kajsiji i šljivi, koje za sada ne predstavlja ozbiljan problem. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja koštičavih voćaka, kako bi se donela pravilna i pravovremena dijagnoza od koje zavisi uspešnost mera zaštite.
PB  - Poljoprivredni fakultet, Novi Sad
T2  - Biljni lekar
T1  - Bakterioze i fitoplazmoze koštičavih voćaka
EP  - 338
IS  - 4-5
SP  - 323
VL  - 38
ER  - 

@article{
author = "Obradović, Aleksa and Kuzmanović, Nemanja and Ćalić, Anđelka and Gašić, Katarina and Ivanović, Milan",
year = "2010",
abstract = "U voćarstvu naše zemlje koštičave voćke zauzimaju značajno mesto i njihovo gajenje predstavlja profitabilnu delatnost. Međutim, rentabilnost voćarske proizvodnje mogu ugroziti različite biljne bolesti. Po značaju, raširenosti, ali i nemogućnosti uspešne kontrole, izdvajaju se oboljenja bakteriozne i fitoplazmozne prirode. Bakteriozno izumiranje, koje prouzrokuje Pseudomo nas syringae sa svoja dva patogena varijeteta syringae i morsprunorum, zadaje ozbiljne probleme voćarima u našoj zemlji. Značajan prob lem, takođe, predstavlja i Agrobacterium tumefaciens, prouzrokovač bakterioznog raka korena, koji nanosi štete u rasadničarskoj proizvodnji. U radu su pred stavljena i oboljenja koja nisu prisutna u našoj zemlji, a u svetu su opisana kao izuzetno destruktivna. To su bakteriozno izumiranje breskve, koje prouzrokuje P. s. pv. persicae i bakteriozna pegavost lišća koštičavih voćaka, čiji je prouzrokovač Xanthomonas arboricola pv. pruni. U Srbiji je prisutno i fitoplazmozno oboljenje Evropsko žutilo koštičavih voćaka na kajsiji i šljivi, koje za sada ne predstavlja ozbiljan problem. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja koštičavih voćaka, kako bi se donela pravilna i pravovremena dijagnoza od koje zavisi uspešnost mera zaštite.",
publisher = "Poljoprivredni fakultet, Novi Sad",
journal = "Biljni lekar",
title = "Bakterioze i fitoplazmoze koštičavih voćaka",
pages = "338-323",
number = "4-5",
volume = "38"
}

Obradović, A., Kuzmanović, N., Ćalić, A., Gašić, K.,& Ivanović, M.. (2010). Bakterioze i fitoplazmoze koštičavih voćaka. in Biljni lekar
Poljoprivredni fakultet, Novi Sad., 38(4-5), 323-338.

Obradović A, Kuzmanović N, Ćalić A, Gašić K, Ivanović M. Bakterioze i fitoplazmoze koštičavih voćaka. in Biljni lekar. 2010;38(4-5):323-338..

Obradović, Aleksa, Kuzmanović, Nemanja, Ćalić, Anđelka, Gašić, Katarina, Ivanović, Milan, "Bakterioze i fitoplazmoze koštičavih voćaka" in Biljni lekar, 38, no. 4-5 (2010):323-338.

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Ivanović, Milan
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1053
AB  - Poslednjih nekoliko godina praćena je pojava nekrotične pegavosti lišća na pojedinačnim stablima smokve u okolini Smederevske Palanke. Tip promena na obolelom lišću ukazivao je na moguću bakterioznu prirodu oboljenja. Kako su se simptomi iz godine u godinu redovno ispoljavali, prikupljani su uzorci lišća i fitopatološki obrađivani, radi utvrđivanja etiologije uočenih promena. Izolacijom iz obolelog tkiva uvek su dobijani sojevi bakterija. Proverom patogenosti izolovanih sojeva, reprodukovani su simptomi prirodne infekcije i potvrđena je bakteriozna priroda oboljenja. Rezultati proučavanja bakterioloških karakteristika ukazuju da se radi o bakteriji Pseudomonas syringae.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine
T2  - Biljni lekar
T1  - Bakteriozna nekrotična pegavost smokve u Srbiji
EP  - 342
IS  - 5
SP  - 338
VL  - 36
ER  - 

@article{
author = "Obradović, Aleksa and Gašić, Katarina and Ivanović, Milan",
year = "2008",
abstract = "Poslednjih nekoliko godina praćena je pojava nekrotične pegavosti lišća na pojedinačnim stablima smokve u okolini Smederevske Palanke. Tip promena na obolelom lišću ukazivao je na moguću bakterioznu prirodu oboljenja. Kako su se simptomi iz godine u godinu redovno ispoljavali, prikupljani su uzorci lišća i fitopatološki obrađivani, radi utvrđivanja etiologije uočenih promena. Izolacijom iz obolelog tkiva uvek su dobijani sojevi bakterija. Proverom patogenosti izolovanih sojeva, reprodukovani su simptomi prirodne infekcije i potvrđena je bakteriozna priroda oboljenja. Rezultati proučavanja bakterioloških karakteristika ukazuju da se radi o bakteriji Pseudomonas syringae.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine",
journal = "Biljni lekar",
title = "Bakteriozna nekrotična pegavost smokve u Srbiji",
pages = "342-338",
number = "5",
volume = "36"
}

Obradović, A., Gašić, K.,& Ivanović, M.. (2008). Bakteriozna nekrotična pegavost smokve u Srbiji. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine., 36(5), 338-342.

Obradović A, Gašić K, Ivanović M. Bakteriozna nekrotična pegavost smokve u Srbiji. in Biljni lekar. 2008;36(5):338-342..

Obradović, Aleksa, Gašić, Katarina, Ivanović, Milan, "Bakteriozna nekrotična pegavost smokve u Srbiji" in Biljni lekar, 36, no. 5 (2008):338-342.