TY  - JOUR
AU  - Mitrović, Petar
AU  - Đalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/605
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Đalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Đalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
MDPI, Basel., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Đalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Đalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .

TY  - JOUR
AU  - Kiprovski, Biljana
AU  - Đalović, Ivica
AU  - Adamović, Dušan
AU  - Mitrović, Petar
AU  - Marjanovic-Jeromela, Ana
AU  - Malencić, Đorđe
AU  - Popović, Tatjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/515
AB  - The aim of the present paper was to study the response of Oenothera biennis L. to 'Candidatus Phytoplasma solani' (Stolbur) infection by analyzing total sugars, polyphenols, photosynthetic pigments content and the antioxidant capacity in leaves and roots of healthy and infected plants. The infection caused a significant increase in peroxidation of lipids, phenylalanine ammonia-lyase activity, total sugar, polyphenols and anthocyanins content (2.8, 2.6, 1.8, 1.4, 6.8 fold, respectively), as well as a decrease in photosynthetic pigments (2-6 fold) and total flavonoids (1.5 fold) in the leaves of Oe. biennis. Changes in these parameters were insignificant in the roots except for the total polyphenols content that was 2.7 times higher in the infected ones. Reduced gluthatione content in both tested organs was not affected by the infection (3.7 and 1.7 mu mol/g fresh weight of leaves and roots, respectively). The elevated content of total sugars, flavonoids and polyphenols, as well as the reduction of photosynthetic pigments and anthocyanins in infected plants are indicative of changes in the metabolism of Oe. biennis affected by the Stolbur phytoplasma. In addition to reduction of chlorophyll and carotenoids, the phytoplasma accelerated leaf senescence. Plants responded to the infection via enhanced superoxide anion scavenging, even though this reaction did not prevent, apparently, membrane damage in analysed leaves. This investigation presents new data on the effect of a phytoplasma infection on its host.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'
EP  - 214
IS  - 2
SP  - 209
VL  - 100
DO  - 10.1007/S42161-018-0068-0
ER  - 

@article{
author = "Kiprovski, Biljana and Đalović, Ivica and Adamović, Dušan and Mitrović, Petar and Marjanovic-Jeromela, Ana and Malencić, Đorđe and Popović, Tatjana",
year = "2018",
abstract = "The aim of the present paper was to study the response of Oenothera biennis L. to 'Candidatus Phytoplasma solani' (Stolbur) infection by analyzing total sugars, polyphenols, photosynthetic pigments content and the antioxidant capacity in leaves and roots of healthy and infected plants. The infection caused a significant increase in peroxidation of lipids, phenylalanine ammonia-lyase activity, total sugar, polyphenols and anthocyanins content (2.8, 2.6, 1.8, 1.4, 6.8 fold, respectively), as well as a decrease in photosynthetic pigments (2-6 fold) and total flavonoids (1.5 fold) in the leaves of Oe. biennis. Changes in these parameters were insignificant in the roots except for the total polyphenols content that was 2.7 times higher in the infected ones. Reduced gluthatione content in both tested organs was not affected by the infection (3.7 and 1.7 mu mol/g fresh weight of leaves and roots, respectively). The elevated content of total sugars, flavonoids and polyphenols, as well as the reduction of photosynthetic pigments and anthocyanins in infected plants are indicative of changes in the metabolism of Oe. biennis affected by the Stolbur phytoplasma. In addition to reduction of chlorophyll and carotenoids, the phytoplasma accelerated leaf senescence. Plants responded to the infection via enhanced superoxide anion scavenging, even though this reaction did not prevent, apparently, membrane damage in analysed leaves. This investigation presents new data on the effect of a phytoplasma infection on its host.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'",
pages = "214-209",
number = "2",
volume = "100",
doi = "10.1007/S42161-018-0068-0"
}

Kiprovski, B., Đalović, I., Adamović, D., Mitrović, P., Marjanovic-Jeromela, A., Malencić, Đ.,& Popović, T.. (2018). Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'. in Journal of Plant Pathology
Springer, New York., 100(2), 209-214.
https://doi.org/10.1007/S42161-018-0068-0

Kiprovski B, Đalović I, Adamović D, Mitrović P, Marjanovic-Jeromela A, Malencić Đ, Popović T. Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'. in Journal of Plant Pathology. 2018;100(2):209-214.
doi:10.1007/S42161-018-0068-0 .

Kiprovski, Biljana, Đalović, Ivica, Adamović, Dušan, Mitrović, Petar, Marjanovic-Jeromela, Ana, Malencić, Đorđe, Popović, Tatjana, "Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'" in Journal of Plant Pathology, 100, no. 2 (2018):209-214,
https://doi.org/10.1007/S42161-018-0068-0 . .