TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Đekić, Ivana
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/127
AB  - In a survey to determine the presence of Phytophthora ramorum in Serbia, ornamentals from garden centers, nurseries, and private and public gardens, as well as imported plant material, were inspected. In total, 577 plant, soil, and potting media samples were tested using various detection methods: lateral flow diagnostic test, enzyme-linked immunosorbent assay, conventional polymerase chain reaction, and isolation, followed by identification based on growth characteristics in culture and morphological features. P. ramorum was not detected in any of the 162 soil or potting media tested by the baiting method. P. ramorum was detected in 12 Rhododendron samples from one private garden in Zemun (City of Belgrade District) exhibiting symptoms of leaf necrosis and blight and petiole necrosis, and in three samples of Pieris spp. from one garden center exhibiting symptoms of leaf necrosis. Eight Phytophthora isolates were obtained from the positive Rhododendron plants and three isolates from Pieris plants, and all were identified as P. ramorum on the basis of their uniform morphological and growth characteristics. P. ramorum conformation was also made by sequencing of the internal transcribed spacer regions for a single isolate taken from one infected rhododendron and one pieris plant. Serbian isolates were determined as A1 mating type, due to formation of a few typical sexual structures when crossed with the A2 mating type of P. cinnamomi and P. cryptogea. Pathogenicity test on non-wounded detached leaves of 19 popular ornamentals, as well as the most frequently imported ones, revealed that 10 host species were susceptible, including Robinia pseudoacacia, which is widely distributed in Serbia. During this study, Cotoneaster horizontalis and C. dammeri were determined to be new experimental hosts of P ramorum. This article provides evidence of P. ramorum introduction into Serbia. Although P. ramorum has not been detected in Serbian production nurseries, its presence outdoors might cause severe damages on susceptible common urban plants in public green and natural ecosystems.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Phytophthora ramorum Occurrence in Ornamentals in Serbia
EP  - 708
IS  - 6
SP  - 703
VL  - 94
DO  - 10.1094/PDIS-94-6-0703
ER  - 

@article{
author = "Bulajić, Aleksandra and Đekić, Ivana and Jović, Jelena and Krnjajić, Slobodan and Vučurović, Ana and Krstić, Branka",
year = "2010",
abstract = "In a survey to determine the presence of Phytophthora ramorum in Serbia, ornamentals from garden centers, nurseries, and private and public gardens, as well as imported plant material, were inspected. In total, 577 plant, soil, and potting media samples were tested using various detection methods: lateral flow diagnostic test, enzyme-linked immunosorbent assay, conventional polymerase chain reaction, and isolation, followed by identification based on growth characteristics in culture and morphological features. P. ramorum was not detected in any of the 162 soil or potting media tested by the baiting method. P. ramorum was detected in 12 Rhododendron samples from one private garden in Zemun (City of Belgrade District) exhibiting symptoms of leaf necrosis and blight and petiole necrosis, and in three samples of Pieris spp. from one garden center exhibiting symptoms of leaf necrosis. Eight Phytophthora isolates were obtained from the positive Rhododendron plants and three isolates from Pieris plants, and all were identified as P. ramorum on the basis of their uniform morphological and growth characteristics. P. ramorum conformation was also made by sequencing of the internal transcribed spacer regions for a single isolate taken from one infected rhododendron and one pieris plant. Serbian isolates were determined as A1 mating type, due to formation of a few typical sexual structures when crossed with the A2 mating type of P. cinnamomi and P. cryptogea. Pathogenicity test on non-wounded detached leaves of 19 popular ornamentals, as well as the most frequently imported ones, revealed that 10 host species were susceptible, including Robinia pseudoacacia, which is widely distributed in Serbia. During this study, Cotoneaster horizontalis and C. dammeri were determined to be new experimental hosts of P ramorum. This article provides evidence of P. ramorum introduction into Serbia. Although P. ramorum has not been detected in Serbian production nurseries, its presence outdoors might cause severe damages on susceptible common urban plants in public green and natural ecosystems.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Phytophthora ramorum Occurrence in Ornamentals in Serbia",
pages = "708-703",
number = "6",
volume = "94",
doi = "10.1094/PDIS-94-6-0703"
}

Bulajić, A., Đekić, I., Jović, J., Krnjajić, S., Vučurović, A.,& Krstić, B.. (2010). Phytophthora ramorum Occurrence in Ornamentals in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 94(6), 703-708.
https://doi.org/10.1094/PDIS-94-6-0703

Bulajić A, Đekić I, Jović J, Krnjajić S, Vučurović A, Krstić B. Phytophthora ramorum Occurrence in Ornamentals in Serbia. in Plant Disease. 2010;94(6):703-708.
doi:10.1094/PDIS-94-6-0703 .

Bulajić, Aleksandra, Đekić, Ivana, Jović, Jelena, Krnjajić, Slobodan, Vučurović, Ana, Krstić, Branka, "Phytophthora ramorum Occurrence in Ornamentals in Serbia" in Plant Disease, 94, no. 6 (2010):703-708,
https://doi.org/10.1094/PDIS-94-6-0703 . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Đekić, Ivana
AU  - Krstić, Branka
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/116
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Pathology
T1  - First report of Phytophthora ramorum on Rhododendron sp in Serbia
EP  - 804
IS  - 4
SP  - 804
VL  - 58
DO  - 10.1111/j.1365-3059.2009.02033.x
ER  - 

@article{
author = "Bulajić, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Đekić, Ivana and Krstić, Branka",
year = "2009",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Pathology",
title = "First report of Phytophthora ramorum on Rhododendron sp in Serbia",
pages = "804-804",
number = "4",
volume = "58",
doi = "10.1111/j.1365-3059.2009.02033.x"
}

Bulajić, A., Jović, J., Krnjajić, S., Đekić, I.,& Krstić, B.. (2009). First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology
Wiley-Blackwell, Hoboken., 58(4), 804-804.
https://doi.org/10.1111/j.1365-3059.2009.02033.x

Bulajić A, Jović J, Krnjajić S, Đekić I, Krstić B. First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology. 2009;58(4):804-804.
doi:10.1111/j.1365-3059.2009.02033.x .

Bulajić, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Đekić, Ivana, Krstić, Branka, "First report of Phytophthora ramorum on Rhododendron sp in Serbia" in Plant Pathology, 58, no. 4 (2009):804-804,
https://doi.org/10.1111/j.1365-3059.2009.02033.x . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Đekić, Ivana
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/101
AB  - In a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Incidence and Distribution of Iris yellow spot virus on Onion in Serbia
EP  - 982
IS  - 10
SP  - 976
VL  - 93
DO  - 10.1094/PDIS-93-10-0976
ER  - 

@article{
author = "Bulajić, Aleksandra and Đekić, Ivana and Jović, Jelena and Krnjajić, Slobodan and Vučurović, Ana and Krstić, Branka",
year = "2009",
abstract = "In a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia",
pages = "982-976",
number = "10",
volume = "93",
doi = "10.1094/PDIS-93-10-0976"
}

Bulajić, A., Đekić, I., Jović, J., Krnjajić, S., Vučurović, A.,& Krstić, B.. (2009). Incidence and Distribution of Iris yellow spot virus on Onion in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 93(10), 976-982.
https://doi.org/10.1094/PDIS-93-10-0976

Bulajić A, Đekić I, Jović J, Krnjajić S, Vučurović A, Krstić B. Incidence and Distribution of Iris yellow spot virus on Onion in Serbia. in Plant Disease. 2009;93(10):976-982.
doi:10.1094/PDIS-93-10-0976 .

Bulajić, Aleksandra, Đekić, Ivana, Jović, Jelena, Krnjajić, Slobodan, Vučurović, Ana, Krstić, Branka, "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia" in Plant Disease, 93, no. 10 (2009):976-982,
https://doi.org/10.1094/PDIS-93-10-0976 . .

Bulajić, A., Jovic, J., Krnjajić, S., Petrov, M., Đekić, I.,& Krstić, B.. (2008). First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease
American Phytopathological Society., 92(8), 1247.
https://doi.org/10.1094/PDIS-92-8-1247A

Bulajić A, Jovic J, Krnjajić S, Petrov M, Đekić I, Krstić B. First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease. 2008;92(8):1247.
doi:10.1094/PDIS-92-8-1247A .

Bulajić, Aleksandra, Jovic, Jelena, Krnjajić, Slobodan, Petrov, M., Đekić, Ivana, Krstić, Branka, "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia" in Plant Disease, 92, no. 8 (2008):1247,
https://doi.org/10.1094/PDIS-92-8-1247A . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Petrov, M.
AU  - Đekić, Ivana
AU  - Krstić, Branka
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/76
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia
EP  - 1247
IS  - 8
SP  - 1247
VL  - 92
DO  - 10.1094/PDIS-92-8-1247A
ER  - 

@article{
author = "Bulajić, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Petrov, M. and Đekić, Ivana and Krstić, Branka",
year = "2008",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia",
pages = "1247-1247",
number = "8",
volume = "92",
doi = "10.1094/PDIS-92-8-1247A"
}

Bulajić, A., Jović, J., Krnjajić, S., Petrov, M., Đekić, I.,& Krstić, B.. (2008). First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 92(8), 1247-1247.
https://doi.org/10.1094/PDIS-92-8-1247A

Bulajić A, Jović J, Krnjajić S, Petrov M, Đekić I, Krstić B. First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease. 2008;92(8):1247-1247.
doi:10.1094/PDIS-92-8-1247A .

Bulajić, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Petrov, M., Đekić, Ivana, Krstić, Branka, "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia" in Plant Disease, 92, no. 8 (2008):1247-1247,
https://doi.org/10.1094/PDIS-92-8-1247A . .

TY  - JOUR
AU  - Đekić, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Krstić, Branka
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Berenji, Janoš
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/93
AB  - Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia.
AB  - Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.
PB  - Novi Sad : Institut za ratarstvo i povrtarstvo
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Frequency and molecular detection of cucumber mosaic virus in tobacco crops
T1  - Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana
EP  - 82
IS  - 81
SP  - 70
VL  - 40
ER  - 

@article{
author = "Đekić, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Krstić, Branka and Jović, Jelena and Krnjajić, Slobodan and Berenji, Janoš",
year = "2008",
abstract = "Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia., Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.",
publisher = "Novi Sad : Institut za ratarstvo i povrtarstvo",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Frequency and molecular detection of cucumber mosaic virus in tobacco crops, Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana",
pages = "82-70",
number = "81",
volume = "40"
}

Đekić, I., Bulajić, A., Vučurović, A., Krstić, B., Jović, J., Krnjajić, S.,& Berenji, J.. (2008). Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje
Novi Sad : Institut za ratarstvo i povrtarstvo., 40(81), 70-82.

Đekić I, Bulajić A, Vučurović A, Krstić B, Jović J, Krnjajić S, Berenji J. Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje. 2008;40(81):70-82..

Đekić, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Krstić, Branka, Jović, Jelena, Krnjajić, Slobodan, Berenji, Janoš, "Frequency and molecular detection of cucumber mosaic virus in tobacco crops" in Bilten za hmelj, sirak i lekovito bilje, 40, no. 81 (2008):70-82.