TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Šević, Milan
AU  - Obradović, Aleksa
AU  - Milošević, Mirjana
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1088
AB  - During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticides and Phytomedicine/Pesticidi i fitomedicina
T1  - Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia
EP  - 149
IS  - 2
SP  - 139
VL  - 25
DO  - 10.2298/PIF1002139I
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Ivanović, Milan and Šević, Milan and Obradović, Aleksa and Milošević, Mirjana",
year = "2010",
abstract = "During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticides and Phytomedicine/Pesticidi i fitomedicina",
title = "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia",
pages = "149-139",
number = "2",
volume = "25",
doi = "10.2298/PIF1002139I"
}

Ignjatov, M., Gašić, K., Ivanović, M., Šević, M., Obradović, A.,& Milošević, M.. (2010). Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticides and Phytomedicine/Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 25(2), 139-149.
https://doi.org/10.2298/PIF1002139I

Ignjatov M, Gašić K, Ivanović M, Šević M, Obradović A, Milošević M. Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticides and Phytomedicine/Pesticidi i fitomedicina. 2010;25(2):139-149.
doi:10.2298/PIF1002139I .

Ignjatov, Maja, Gašić, Katarina, Ivanović, Milan, Šević, Milan, Obradović, Aleksa, Milošević, Mirjana, "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia" in Pesticides and Phytomedicine/Pesticidi i fitomedicina, 25, no. 2 (2010):139-149,
https://doi.org/10.2298/PIF1002139I . .

TY  - CHAP
AU  - Ignjatov, Maja
AU  - Balaž, Jelica
AU  - Milošević, Mirjana
AU  - Vidić, Miloš
AU  - Popović Milovanović, Tatjana
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1056
AB  - Bacterial blight is a disease of soybean appearing regularly with high intensity in Serbia. Isolation of pathogen was done using beef extract (MPA) and the medium enriched with sucrose (NSA) from infected soybean leaves of several varieties grown in Vojvodina province, during 2005.
Only the representative isolates were chosen for analyses. Pathogenicity of the isolates including the reference strain (National Collection of Plant Pathogenic Bacteria, United Kingdom-NCPPB 3318) was determined by inoculating soybean plants at cotyledon stage (cv. Balkan), by dipping and spraying with bacterial suspensions (conc. 108 CFU/ml). Pathogenicity of isolates was also confirmed on tobacco plants (HR positive reaction).
Cultural, biochemical and physiological characteristics were tested according to methods cited by Lelliot and Stead (1987), Klement et al. (1990), Schaad (1980). The colonies are chosen by fluorescence on KB agar, and by the LOPAT tests (levan production, oxidase reaction, potato rot, arginine dihydrolase production and tobacco hypersensitivity: +, −, −, −, +). The studied isolates of bacterium did not hydrolyze starch, gelatine and aesculin. They did not reduce nitrate but metabolized glucose oxidatively and were catalase positive. All the isolates produced acid from glucose, galactose, xylose, arabinose, manose, sucrose, rafinose and inositol.
Bacterium identification was confirmed by a serological method (agglutination tests by Express Kit – NEOGEN Europe Ltd., Scotland, UK), with appropriate antibodies of Pseudomonas syringae pv. glycinea. The studied isolates formed whitish sediment in the case of positive reaction.
Differential soybean sortiment: Acme, Chippewa, Flambeau, Harosoy, Lindarin, Merit and Norchief was used to determine which physiological race the isolates belonged to. Young plants were inoculated in two ways: by rubbing leaves with sterile cotton swab dipped in an aqueous bacterial suspension (Alvarez et al., 1995), and by spraying leaves under pressure (Cross et al., 1966; Balaž et al., 1990; Prom and Venette, 1997).
Based on the results of these studies we concluded that strains which cause blight of soybeans obtained from Vojvodina, belong to Pseudomonas syringae pv. glycinea, race 4.
PB  - Springer, Dordrecht
T2  - In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht
T1  - Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.)
DO  - 10.1007/978-1-4020-6901-7_42
ER  - 

@inbook{
author = "Ignjatov, Maja and Balaž, Jelica and Milošević, Mirjana and Vidić, Miloš and Popović Milovanović, Tatjana",
year = "2008",
abstract = "Bacterial blight is a disease of soybean appearing regularly with high intensity in Serbia. Isolation of pathogen was done using beef extract (MPA) and the medium enriched with sucrose (NSA) from infected soybean leaves of several varieties grown in Vojvodina province, during 2005.
Only the representative isolates were chosen for analyses. Pathogenicity of the isolates including the reference strain (National Collection of Plant Pathogenic Bacteria, United Kingdom-NCPPB 3318) was determined by inoculating soybean plants at cotyledon stage (cv. Balkan), by dipping and spraying with bacterial suspensions (conc. 108 CFU/ml). Pathogenicity of isolates was also confirmed on tobacco plants (HR positive reaction).
Cultural, biochemical and physiological characteristics were tested according to methods cited by Lelliot and Stead (1987), Klement et al. (1990), Schaad (1980). The colonies are chosen by fluorescence on KB agar, and by the LOPAT tests (levan production, oxidase reaction, potato rot, arginine dihydrolase production and tobacco hypersensitivity: +, −, −, −, +). The studied isolates of bacterium did not hydrolyze starch, gelatine and aesculin. They did not reduce nitrate but metabolized glucose oxidatively and were catalase positive. All the isolates produced acid from glucose, galactose, xylose, arabinose, manose, sucrose, rafinose and inositol.
Bacterium identification was confirmed by a serological method (agglutination tests by Express Kit – NEOGEN Europe Ltd., Scotland, UK), with appropriate antibodies of Pseudomonas syringae pv. glycinea. The studied isolates formed whitish sediment in the case of positive reaction.
Differential soybean sortiment: Acme, Chippewa, Flambeau, Harosoy, Lindarin, Merit and Norchief was used to determine which physiological race the isolates belonged to. Young plants were inoculated in two ways: by rubbing leaves with sterile cotton swab dipped in an aqueous bacterial suspension (Alvarez et al., 1995), and by spraying leaves under pressure (Cross et al., 1966; Balaž et al., 1990; Prom and Venette, 1997).
Based on the results of these studies we concluded that strains which cause blight of soybeans obtained from Vojvodina, belong to Pseudomonas syringae pv. glycinea, race 4.",
publisher = "Springer, Dordrecht",
journal = "In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht",
booktitle = "Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.)",
doi = "10.1007/978-1-4020-6901-7_42"
}

Ignjatov, M., Balaž, J., Milošević, M., Vidić, M.,& Popović Milovanović, T.. (2008). Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.). in In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht
Springer, Dordrecht..
https://doi.org/10.1007/978-1-4020-6901-7_42

Ignjatov M, Balaž J, Milošević M, Vidić M, Popović Milovanović T. Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.). in In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht. 2008;.
doi:10.1007/978-1-4020-6901-7_42 .

Ignjatov, Maja, Balaž, Jelica, Milošević, Mirjana, Vidić, Miloš, Popović Milovanović, Tatjana, "Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.)" in In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht (2008),
https://doi.org/10.1007/978-1-4020-6901-7_42 . .