TY  - CHAP
AU  - Ignjatov, Maja
AU  - Balaž, Jelica
AU  - Milošević, Mirjana
AU  - Vidić, Miloš
AU  - Popović Milovanović, Tatjana
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1056
AB  - Bacterial blight is a disease of soybean appearing regularly with high intensity in Serbia. Isolation of pathogen was done using beef extract (MPA) and the medium enriched with sucrose (NSA) from infected soybean leaves of several varieties grown in Vojvodina province, during 2005.
Only the representative isolates were chosen for analyses. Pathogenicity of the isolates including the reference strain (National Collection of Plant Pathogenic Bacteria, United Kingdom-NCPPB 3318) was determined by inoculating soybean plants at cotyledon stage (cv. Balkan), by dipping and spraying with bacterial suspensions (conc. 108 CFU/ml). Pathogenicity of isolates was also confirmed on tobacco plants (HR positive reaction).
Cultural, biochemical and physiological characteristics were tested according to methods cited by Lelliot and Stead (1987), Klement et al. (1990), Schaad (1980). The colonies are chosen by fluorescence on KB agar, and by the LOPAT tests (levan production, oxidase reaction, potato rot, arginine dihydrolase production and tobacco hypersensitivity: +, −, −, −, +). The studied isolates of bacterium did not hydrolyze starch, gelatine and aesculin. They did not reduce nitrate but metabolized glucose oxidatively and were catalase positive. All the isolates produced acid from glucose, galactose, xylose, arabinose, manose, sucrose, rafinose and inositol.
Bacterium identification was confirmed by a serological method (agglutination tests by Express Kit – NEOGEN Europe Ltd., Scotland, UK), with appropriate antibodies of Pseudomonas syringae pv. glycinea. The studied isolates formed whitish sediment in the case of positive reaction.
Differential soybean sortiment: Acme, Chippewa, Flambeau, Harosoy, Lindarin, Merit and Norchief was used to determine which physiological race the isolates belonged to. Young plants were inoculated in two ways: by rubbing leaves with sterile cotton swab dipped in an aqueous bacterial suspension (Alvarez et al., 1995), and by spraying leaves under pressure (Cross et al., 1966; Balaž et al., 1990; Prom and Venette, 1997).
Based on the results of these studies we concluded that strains which cause blight of soybeans obtained from Vojvodina, belong to Pseudomonas syringae pv. glycinea, race 4.
PB  - Springer, Dordrecht
T2  - In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht
T1  - Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.)
DO  - 10.1007/978-1-4020-6901-7_42
ER  - 

@inbook{
author = "Ignjatov, Maja and Balaž, Jelica and Milošević, Mirjana and Vidić, Miloš and Popović Milovanović, Tatjana",
year = "2008",
abstract = "Bacterial blight is a disease of soybean appearing regularly with high intensity in Serbia. Isolation of pathogen was done using beef extract (MPA) and the medium enriched with sucrose (NSA) from infected soybean leaves of several varieties grown in Vojvodina province, during 2005.
Only the representative isolates were chosen for analyses. Pathogenicity of the isolates including the reference strain (National Collection of Plant Pathogenic Bacteria, United Kingdom-NCPPB 3318) was determined by inoculating soybean plants at cotyledon stage (cv. Balkan), by dipping and spraying with bacterial suspensions (conc. 108 CFU/ml). Pathogenicity of isolates was also confirmed on tobacco plants (HR positive reaction).
Cultural, biochemical and physiological characteristics were tested according to methods cited by Lelliot and Stead (1987), Klement et al. (1990), Schaad (1980). The colonies are chosen by fluorescence on KB agar, and by the LOPAT tests (levan production, oxidase reaction, potato rot, arginine dihydrolase production and tobacco hypersensitivity: +, −, −, −, +). The studied isolates of bacterium did not hydrolyze starch, gelatine and aesculin. They did not reduce nitrate but metabolized glucose oxidatively and were catalase positive. All the isolates produced acid from glucose, galactose, xylose, arabinose, manose, sucrose, rafinose and inositol.
Bacterium identification was confirmed by a serological method (agglutination tests by Express Kit – NEOGEN Europe Ltd., Scotland, UK), with appropriate antibodies of Pseudomonas syringae pv. glycinea. The studied isolates formed whitish sediment in the case of positive reaction.
Differential soybean sortiment: Acme, Chippewa, Flambeau, Harosoy, Lindarin, Merit and Norchief was used to determine which physiological race the isolates belonged to. Young plants were inoculated in two ways: by rubbing leaves with sterile cotton swab dipped in an aqueous bacterial suspension (Alvarez et al., 1995), and by spraying leaves under pressure (Cross et al., 1966; Balaž et al., 1990; Prom and Venette, 1997).
Based on the results of these studies we concluded that strains which cause blight of soybeans obtained from Vojvodina, belong to Pseudomonas syringae pv. glycinea, race 4.",
publisher = "Springer, Dordrecht",
journal = "In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht",
booktitle = "Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.)",
doi = "10.1007/978-1-4020-6901-7_42"
}

Ignjatov, M., Balaž, J., Milošević, M., Vidić, M.,& Popović Milovanović, T.. (2008). Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.). in In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht
Springer, Dordrecht..
https://doi.org/10.1007/978-1-4020-6901-7_42

Ignjatov M, Balaž J, Milošević M, Vidić M, Popović Milovanović T. Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.). in In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht. 2008;.
doi:10.1007/978-1-4020-6901-7_42 .

Ignjatov, Maja, Balaž, Jelica, Milošević, Mirjana, Vidić, Miloš, Popović Milovanović, Tatjana, "Studies on Plant Pathogenic Bacterium Causal Agent of Soybean Bacterial Spots (Pseudomonas syringaepv. glycinea (Coerper) Young et al.)" in In: Fatmi, M., et al. Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer, Dordrecht (2008),
https://doi.org/10.1007/978-1-4020-6901-7_42 . .