TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1137
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Vrandečić, Karolina
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/875
AB  - Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia
EP  - 176
IS  - 1
SP  - 159
VL  - 55
DO  - 10.2298/gensr2301159i
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Blagojević, Milan and Bagi, Ferenc and Vrandečić, Karolina and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia",
pages = "176-159",
number = "1",
volume = "55",
doi = "10.2298/gensr2301159i"
}

Iličić, R., Jelušić, A., Blagojević, M., Bagi, F., Vrandečić, K., Stanković, S.,& Popović Milovanović, T.. (2023). Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika
Serbian Genetics Society., 55(1), 159-176.
https://doi.org/10.2298/gensr2301159i

Iličić R, Jelušić A, Blagojević M, Bagi F, Vrandečić K, Stanković S, Popović Milovanović T. Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika. 2023;55(1):159-176.
doi:10.2298/gensr2301159i .

Iličić, Renata, Jelušić, Aleksandra, Blagojević, Milan, Bagi, Ferenc, Vrandečić, Karolina, Stanković, Slaviša, Popović Milovanović, Tatjana, "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia" in Genetika, 55, no. 1 (2023):159-176,
https://doi.org/10.2298/gensr2301159i . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Medić, O.
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/849
AB  - Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists' potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.
PB  - Elsevier
T2  - Biological Control
T1  - Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4
SP  - 105238
VL  - 182
DO  - 10.1016/j.biocontrol.2023.105238
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Medić, O. and Berić, Tanja and Stanković, Slaviša",
year = "2023",
abstract = "Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists' potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4",
pages = "105238",
volume = "182",
doi = "10.1016/j.biocontrol.2023.105238"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Medić, O., Berić, T.,& Stanković, S.. (2023). Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control
Elsevier., 182, 105238.
https://doi.org/10.1016/j.biocontrol.2023.105238

Marković S, Popović Milovanović T, Jelušić A, Medić O, Berić T, Stanković S. Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control. 2023;182:105238.
doi:10.1016/j.biocontrol.2023.105238 .

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Medić, O., Berić, Tanja, Stanković, Slaviša, "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4" in Biological Control, 182 (2023):105238,
https://doi.org/10.1016/j.biocontrol.2023.105238 . .

TY  - JOUR
AU  - Jankiev, Tamara
AU  - Berić, Tanja
AU  - Stević, Tatjana
AU  - Stanković, Slaviša
AU  - Bačić, Jasmina
AU  - Majstorović, Helena
AU  - Fira, Đorđe
AU  - Dimkić, Ivica
PY  - 2022
UR  - https://enauka.gov.rs/handle/123456789/779167
UR  - https://intam.institut-tamis.rs/handle/123456789/218
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1190
AB  - The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two phenological stages during fruit development and maturation. The antagonistic potential of autochthonous bacterial and yeast isolates against phytopathogenic fungi was also evaluated. A metabarcoding approach revealed Pantoea, Sphingomonas, Hymenobacter, Massilia, and Pseudomonas as dominant bacterial constituents of the pear phyllosphere, whilst most abundant among the fungal representatives identified were Metschnikowia, Filobasidium, Aureobasidium pullulans, Botrytis cinerea, and Taphrina. The traditional culturable approach revealed that the Pseudomonas genus with P. graminis, P. putida, and P. congelans was most prevalent. The most frequently cultivated fungal representatives belonged to the genus Fusarium with six identified species. A broad range of the antagonistic activity was detected for the Hannaella luteola and Metschnikowia pulcherrima yeasts, significantly affecting the growth of many fungal isolates in the range of 53-70%. Fusarium sporotrichioides was the most susceptible fungal isolate. The autochthonous antagonistic yeasts H. luteola and M. pulcherrima might be powerful biological control agents of postharvest diseases caused by Fusarium spp. and common pathogens like Monilinia laxa, Botrytis cinerea, Alternaria tenuissima, and Cladosporium cladosporioides.
PB  - MDPI
T2  - Microorganisms
T1  - The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates
IS  - 7
SP  - 1282
VL  - 10
DO  - 10.3390/microorganisms10071282
ER  - 

@article{
author = "Jankiev, Tamara and Berić, Tanja and Stević, Tatjana and Stanković, Slaviša and Bačić, Jasmina and Majstorović, Helena and Fira, Đorđe and Dimkić, Ivica",
year = "2022",
abstract = "The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two phenological stages during fruit development and maturation. The antagonistic potential of autochthonous bacterial and yeast isolates against phytopathogenic fungi was also evaluated. A metabarcoding approach revealed Pantoea, Sphingomonas, Hymenobacter, Massilia, and Pseudomonas as dominant bacterial constituents of the pear phyllosphere, whilst most abundant among the fungal representatives identified were Metschnikowia, Filobasidium, Aureobasidium pullulans, Botrytis cinerea, and Taphrina. The traditional culturable approach revealed that the Pseudomonas genus with P. graminis, P. putida, and P. congelans was most prevalent. The most frequently cultivated fungal representatives belonged to the genus Fusarium with six identified species. A broad range of the antagonistic activity was detected for the Hannaella luteola and Metschnikowia pulcherrima yeasts, significantly affecting the growth of many fungal isolates in the range of 53-70%. Fusarium sporotrichioides was the most susceptible fungal isolate. The autochthonous antagonistic yeasts H. luteola and M. pulcherrima might be powerful biological control agents of postharvest diseases caused by Fusarium spp. and common pathogens like Monilinia laxa, Botrytis cinerea, Alternaria tenuissima, and Cladosporium cladosporioides.",
publisher = "MDPI",
journal = "Microorganisms",
title = "The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates",
number = "7",
pages = "1282",
volume = "10",
doi = "10.3390/microorganisms10071282"
}

Jankiev, T., Berić, T., Stević, T., Stanković, S., Bačić, J., Majstorović, H., Fira, Đ.,& Dimkić, I.. (2022). The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates. in Microorganisms
MDPI., 10(7), 1282.
https://doi.org/10.3390/microorganisms10071282

Jankiev T, Berić T, Stević T, Stanković S, Bačić J, Majstorović H, Fira Đ, Dimkić I. The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates. in Microorganisms. 2022;10(7):1282.
doi:10.3390/microorganisms10071282 .

Jankiev, Tamara, Berić, Tanja, Stević, Tatjana, Stanković, Slaviša, Bačić, Jasmina, Majstorović, Helena, Fira, Đorđe, Dimkić, Ivica, "The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates" in Microorganisms, 10, no. 7 (2022):1282,
https://doi.org/10.3390/microorganisms10071282 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajić, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/674
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije
T2  - Genetika
T1  - Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Iličić, Renata and Popović Milovanović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajić, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije",
journal = "Genetika",
title = "Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Iličić, R., Popović Milovanović, T., Marković, S., Jelušić, A., Bagi, F., Vlajić, S.,& Stanković, S.. (2021). Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika
Društvo genetičara Srbije., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Iličić R, Popović Milovanović T, Marković S, Jelušić A, Bagi F, Vlajić S, Stanković S. Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Iličić, Renata, Popović Milovanović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajić, Slobodan, Stanković, Slaviša, "Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanovic Jeromela, Ana
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/942
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1458
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanovic Jeromela, Ana and Nikolić, Ivan and Stanković, Slaviša",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1458-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović Milovanović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanovic Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley., 68(8), 1448-1458.
https://doi.org/10.1111/ppa.13064

Popović Milovanović T, Mitrović P, Jelušić A, Dimkić I, Marjanovic Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1458.
doi:10.1111/ppa.13064 .

Popović Milovanović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanovic Jeromela, Ana, Nikolić, Ivan, Stanković, Slaviša, "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1458,
https://doi.org/10.1111/ppa.13064 . .

TY  - CHAP
AU  - Ivanović, Žarko
AU  - Gavrilovic, Veljko
AU  - Popović Milovanović, Tatjana
AU  - Zivkovic, Svetlana
AU  - Blagojević, Jovana
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1074
PB  - Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India
T2  - Technological Advancement for Vibrant Agriculture
T1  - Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates
EP  - 159
SP  - 153
VL  - 7
ER  - 

@inbook{
author = "Ivanović, Žarko and Gavrilovic, Veljko and Popović Milovanović, Tatjana and Zivkovic, Svetlana and Blagojević, Jovana and Stanković, Slaviša and Fira, Đorđe",
year = "2014",
publisher = "Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India",
journal = "Technological Advancement for Vibrant Agriculture",
booktitle = "Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates",
pages = "159-153",
volume = "7"
}

Ivanović, Ž., Gavrilovic, V., Popović Milovanović, T., Zivkovic, S., Blagojević, J., Stanković, S.,& Fira, Đ.. (2014). Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates. in Technological Advancement for Vibrant Agriculture
Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India., 7, 153-159.

Ivanović Ž, Gavrilovic V, Popović Milovanović T, Zivkovic S, Blagojević J, Stanković S, Fira Đ. Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates. in Technological Advancement for Vibrant Agriculture. 2014;7:153-159..

Ivanović, Žarko, Gavrilovic, Veljko, Popović Milovanović, Tatjana, Zivkovic, Svetlana, Blagojević, Jovana, Stanković, Slaviša, Fira, Đorđe, "Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates" in Technological Advancement for Vibrant Agriculture, 7 (2014):153-159.