TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Aćimović, Radivoje
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/809
AB  - The vegetable soybean “edamame” (Glicine max L.) is a nutritious legume having pods and seeds that can be harvested and consumed while they are still fresh and premature. Edamame is rich in micronutrients and vitamins and is therefore used as food and for medicinal purposes. In Serbia, edamame production started for the first time in 2019 on a 10 ha field in Bečej locality (Bačka, Vojvodina). Later, in 2021, its production was expanded to 30 ha, but in summer was followed with symptoms of bacterial leaf spot. The symptoms appeared on leaves in the form of water-soaked spots surrounded by a chlorotic halo that enlarged and coalesced into necrotic lesions. Disease incidence was from 15-20%. Isolation of the causal pathogen was performed by sowing of suspension of the macerated margins of spots/lesions from ten collected symptomatic leaves on nutrient agar supplemented with 5% sucrose. Ten representative, purified isolates were whitish, circular, smooth, shiny, levan-positive, strictly aerobic, gram-negative; positive for green-fluorescent pigment and tobacco hypersensitive response, and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Pathogenicity of the isolates was confirmed on soybean cotyledons by under pressure atomizing a bacterial suspension (107-8 CFU mL 1 ). Sequencing of genes gapA, gyrB, and rpoD showed 100% homology of the obtained isolates with Pseudomonas savastanoi pv. glycinea pathotype strain LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, MOC601, R4a (gapA and gyrB), and M301765 (gapA and rpoD) originated from soybean, all from the Plant Associated and Environmental Microbes Database (PAMDB).
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124
T1  - Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Jelušić, Aleksandra and Aćimović, Radivoje and Marković, Sanja and Iličić, Renata",
year = "2022",
abstract = "The vegetable soybean “edamame” (Glicine max L.) is a nutritious legume having pods and seeds that can be harvested and consumed while they are still fresh and premature. Edamame is rich in micronutrients and vitamins and is therefore used as food and for medicinal purposes. In Serbia, edamame production started for the first time in 2019 on a 10 ha field in Bečej locality (Bačka, Vojvodina). Later, in 2021, its production was expanded to 30 ha, but in summer was followed with symptoms of bacterial leaf spot. The symptoms appeared on leaves in the form of water-soaked spots surrounded by a chlorotic halo that enlarged and coalesced into necrotic lesions. Disease incidence was from 15-20%. Isolation of the causal pathogen was performed by sowing of suspension of the macerated margins of spots/lesions from ten collected symptomatic leaves on nutrient agar supplemented with 5% sucrose. Ten representative, purified isolates were whitish, circular, smooth, shiny, levan-positive, strictly aerobic, gram-negative; positive for green-fluorescent pigment and tobacco hypersensitive response, and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Pathogenicity of the isolates was confirmed on soybean cotyledons by under pressure atomizing a bacterial suspension (107-8 CFU mL 1 ). Sequencing of genes gapA, gyrB, and rpoD showed 100% homology of the obtained isolates with Pseudomonas savastanoi pv. glycinea pathotype strain LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, MOC601, R4a (gapA and gyrB), and M301765 (gapA and rpoD) originated from soybean, all from the Plant Associated and Environmental Microbes Database (PAMDB).",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124",
title = "Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia"
}

Popović Milovanović, T., Jelušić, A., Aćimović, R., Marković, S.,& Iličić, R.. (2022). Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia. in 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini..

Popović Milovanović T, Jelušić A, Aćimović R, Marković S, Iličić R. Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia. in 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124. 2022;..

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Aćimović, Radivoje, Marković, Sanja, Iličić, Renata, "Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia" in 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124 (2022).

TY  - CONF
AU  - Iličić, Renata
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Vlajić, Slobodan
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1342
AB  - Bacterial canker caused by Pseudomonas syringae pathovars is one of the most serious cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly based from imported planting material are being raised every year. In general, bacterial canker is mostly distributed in young sweet cherry plantations worldwide and in Serbia also. During spring, in May 2018 and 2019, occurrence of bacterial canker symptoms was observed on a newly planted sweet cherry plantations on two localities, Zitorada and Karavukovo. Therefore the aim of this work was to determine causal agent of the disease using conventional and molecular tools. 
Typical P. syringae colonies were isolated on Nutrient Sucrose Agar from all collected sweet cher­ry samples with expressed bacterial canker or leaf spot symptoms. All isolates were Gram negative, catalase positive, produced fluorescent pigment on King B medium under UV light and showed oxidative metabolism of glucose. Results of the LOPAT test (+---+) determined isolates to belong to fluorescent Pseudomonas Group la, while in G•A•TTa· showed characteristics described for pv. syringae. Pathogenicity was confirmed on immature fruits of sweet and sour cherry where all isolates caused typical symptoms in form of black, sunken lesions, characteristics for pv. syringae. Selected bacterial isolates were further identified by DNA sequencing using gyrB housekeeping gene. All tested isolates were identified as P. syringae pv. syringae using NCBI BLAST. Isolate REOS, isolated from sweet cherry stem (Karavukovo) showed 9g.51% homology with P. syringae pv. syringae strain CFBP421 S (sweet cherry, France). Isolates RE3 and RE4 (Zitorada) originated from branch and leaf, respectively, showed 99.19% homology with P. syringae pv. syringae strain Pss9097 (sweet cherry, UK) and CFBP2118 (sweet cherry, France). Phylogenetic analysis grouped isolates from 2itorada (RE3 and RE4) in one tree cluster, separate from the isolate REOS, showing presence of two genetically diverse groups of P. syringae pv. syringae isolates, obtained from two geographically distinct localities in Serbia. 
Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check needs to be in­cluded as obligatory measure when nursery materials is used from import.
PB  - Društvo za zaštitu bilja
C3  - VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia
T1  - NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS
SP  - 185
ER  - 

@conference{
author = "Iličić, Renata and Popović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Vlajić, Slobodan",
year = "2019",
abstract = "Bacterial canker caused by Pseudomonas syringae pathovars is one of the most serious cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly based from imported planting material are being raised every year. In general, bacterial canker is mostly distributed in young sweet cherry plantations worldwide and in Serbia also. During spring, in May 2018 and 2019, occurrence of bacterial canker symptoms was observed on a newly planted sweet cherry plantations on two localities, Zitorada and Karavukovo. Therefore the aim of this work was to determine causal agent of the disease using conventional and molecular tools. 
Typical P. syringae colonies were isolated on Nutrient Sucrose Agar from all collected sweet cher­ry samples with expressed bacterial canker or leaf spot symptoms. All isolates were Gram negative, catalase positive, produced fluorescent pigment on King B medium under UV light and showed oxidative metabolism of glucose. Results of the LOPAT test (+---+) determined isolates to belong to fluorescent Pseudomonas Group la, while in G•A•TTa· showed characteristics described for pv. syringae. Pathogenicity was confirmed on immature fruits of sweet and sour cherry where all isolates caused typical symptoms in form of black, sunken lesions, characteristics for pv. syringae. Selected bacterial isolates were further identified by DNA sequencing using gyrB housekeeping gene. All tested isolates were identified as P. syringae pv. syringae using NCBI BLAST. Isolate REOS, isolated from sweet cherry stem (Karavukovo) showed 9g.51% homology with P. syringae pv. syringae strain CFBP421 S (sweet cherry, France). Isolates RE3 and RE4 (Zitorada) originated from branch and leaf, respectively, showed 99.19% homology with P. syringae pv. syringae strain Pss9097 (sweet cherry, UK) and CFBP2118 (sweet cherry, France). Phylogenetic analysis grouped isolates from 2itorada (RE3 and RE4) in one tree cluster, separate from the isolate REOS, showing presence of two genetically diverse groups of P. syringae pv. syringae isolates, obtained from two geographically distinct localities in Serbia. 
Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check needs to be in­cluded as obligatory measure when nursery materials is used from import.",
publisher = "Društvo za zaštitu bilja",
journal = "VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia",
title = "NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS",
pages = "185"
}

Iličić, R., Popović, T., Jelušić, A., Marković, S.,& Vlajić, S.. (2019). NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia
Društvo za zaštitu bilja., 185.

Iličić R, Popović T, Jelušić A, Marković S, Vlajić S. NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia. 2019;:185..

Iličić, Renata, Popović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Vlajić, Slobodan, "NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS" in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia (2019):185.