@conference{
author = "Iličić, Renata and Barać, Goran and Bagi, Ferenc F. and Vlajić, Slobodan and Popović Milovanović, Tatjana",
year = "2022",
abstract = "Bacterial leaf spot, caused by the plant pathogenic bacterium Pseudomonas cerasi is an
economic threat to Prunus production worldwide. This bacterium was described in 2016
for the first time as a pathogen of wild cherry in Serbia. With the aim to determine the
host specificity within Prunus plant species young detached leaves were inoculated using
a method of needleless syringe. Ten Prunus species were tested: P. avium, P. armeniaca,
P. cerasus, P. cerasifera, P. domestica, P. dulcis, P. fruticosa, P. mahaleb, P. spinosa and
P. persica. Strain of P. cerasi (RE10 originated from wild cherry leaves, Rimski Sancevi)
grown on Nutrient Agar {26°C for 48 h), and suspended in sterile distilled water (SDW) to
the final concentration of 107
CFU mL·
1
was used as bacterial inoculum. Control leaves
were inoculated with SDW. The inoculated leaves were maintained in plastic boxes at
ambient temperature. Symptom development in the form of bacterial spot-necrosis was
rated five times, every two days until 10 days after inoculation (DAI). Results were
expressed as the measured diameters of the spot-necrosis (rnrn), and average value was
analyzed. Host range assays showed that developed symptoms caused by P. cerasi strain
RE10 varied among ten tested Prunus species. Two DAI the symptom appeared only on
leaves of P. domestica (3.5). Further, 4 DAI, symptoms were recorded on P. avium {4.9L
P. cerasus {4.9L P. fruticosa {4.66L P. cerasifera {3.88L P. spinosa (3.88) and P. persica
{4.88L and on P. domestica necrosis enlarged (4.55). Leaves of P. mahaleb, P. armeniaca
and P. dulcis were symptomless. Six DAI spots enlarged and reached 3.66; 4.44; 4.55;
4.77; 4.77; 5.10; 5.22 and 5.54 mm for P. dulcis, P. cerasifera, P. spinosa, P. fruticosa, P.
domestica, P. avium, P. cerasus, and P. persica, respectively. P. armeniaca and P.
mahaleb were symptomless. Eight DAI the presence of necrotic spots was noted on P.
mahaleb (2.77) leaves. At the final rating, 10 DAI, no differences were found in formed
spot-necrosis on P. mahaleb {2.77L P. dulcis {4.33L P. cerasifera {4.66L P. fruticosa
{5.10L P. avium {5.33L P. spinosa (5.55) and P. cerasus (5.66). Only diameter of spotnecrosis enlarges for P. domestica (5.65) and P. persica (9.11). Leaves of P. armeniaca
were symptomless until the end of the experiment. During the whole experiment, no
symptoms were found in negative controls. Results obtained in this research provide the
significant data of potential risk for further spread of P. cerasi among Prunus species.
However, P. armeniaca could be recommended to serve as a source for future breeding programs considering that this Prunus did not result with susceptibility to bacterium P.
cerasi.",
publisher = "University of Novi Sad - Faculty of Agriculture",
journal = "16 th Serbian Congress of Fruit and Grapevine Producers, with International Participation, February 28 - March 3 2022, Vrdnik, Serbia",
title = "Evidence of Prunus host specificity of Pseudomonas cerasi based on detached leaves pathogenicity assay",
pages = "244"
}
