TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Iličić, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/740
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.
PB  - British Society for Plant Pathology
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
DO  - 0032-0862
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Iličić, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.",
publisher = "British Society for Plant Pathology",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421, 0032-0862"
}

Marković, S., Stanković, S., Iličić, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović Milovanović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
British Society for Plant Pathology., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Iličić R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović Milovanović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Iličić, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović Milovanović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .