TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/550
AB  - The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions
EP  - 64
IS  - 1
SP  - 47
VL  - 172
DO  - 10.1111/aab.12400
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Radonjić, Sanja and Hrnčić, Snježana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions",
pages = "64-47",
number = "1",
volume = "172",
doi = "10.1111/aab.12400"
}

Krstić, O., Cvrković, T., Mitrović, M., Radonjić, S., Hrnčić, S., Toševski, I.,& Jović, J.. (2018). Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology
Wiley, Hoboken., 172(1), 47-64.
https://doi.org/10.1111/aab.12400

Krstić O, Cvrković T, Mitrović M, Radonjić S, Hrnčić S, Toševski I, Jović J. Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology. 2018;172(1):47-64.
doi:10.1111/aab.12400 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Radonjić, Sanja, Hrnčić, Snježana, Toševski, Ivo, Jović, Jelena, "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions" in Annals of Applied Biology, 172, no. 1 (2018):47-64,
https://doi.org/10.1111/aab.12400 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/439
AB  - Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)
EP  - 298
IS  - 2
SP  - 285
VL  - 65
DO  - 10.1111/ppa.12409
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)",
pages = "298-285",
number = "2",
volume = "65",
doi = "10.1111/ppa.12409"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology
Wiley, Hoboken., 65(2), 285-298.
https://doi.org/10.1111/ppa.12409

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology. 2016;65(2):285-298.
doi:10.1111/ppa.12409 .

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)" in Plant Pathology, 65, no. 2 (2016):285-298,
https://doi.org/10.1111/ppa.12409 . .