Ivanović, Ž., Milošević, D., Ignjatov, M., Marjanovic Jeromela, A., Karaman, M.,& Grahovac, M.. (2020). First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease
American Phytopathological Society., 104(9), 2516-2516.
https://doi.org/10.1094/PDIS-03-20-0602-PDN

Ivanović Ž, Milošević D, Ignjatov M, Marjanovic Jeromela A, Karaman M, Grahovac M. First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease. 2020;104(9):2516-2516.
doi:10.1094/PDIS-03-20-0602-PDN .

Ivanović, Žarko, Milošević, Dragana, Ignjatov, Maja, Marjanovic Jeromela, Ana, Karaman, Maja, Grahovac, Mila, "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia" in Plant Disease, 104, no. 9 (2020):2516-2516,
https://doi.org/10.1094/PDIS-03-20-0602-PDN . .

Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Stojanović, M., Popović, V.,& Ivanović, Ž.. (2020). First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease, 104(6), 1864-1864.
https://doi.org/10.1094/PDIS-10-19-2218-PDN

Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Stojanović M, Popović V, Ivanović Ž. First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease. 2020;104(6):1864-1864.
doi:10.1094/PDIS-10-19-2218-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Stojanović, Milan, Popović, Vera, Ivanović, Žarko, "First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia" in Plant Disease, 104, no. 6 (2020):1864-1864,
https://doi.org/10.1094/PDIS-10-19-2218-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/608
AB  - Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.
AB  - Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L
T1  - Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L
IS  - 137
SP  - 49
DO  - 10.2298/ZMSPN1937049I
ER  - 

@article{
author = "Ignjatov, Maja and Vlajić, Slobodan and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2019",
abstract = "Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves., Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L, Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L",
number = "137",
pages = "49",
doi = "10.2298/ZMSPN1937049I"
}

Ignjatov, M., Vlajić, S., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2019). Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(137), 49.
https://doi.org/10.2298/ZMSPN1937049I

Ignjatov M, Vlajić S, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke. 2019;(137):49.
doi:10.2298/ZMSPN1937049I .

Ignjatov, Maja, Vlajić, Slobodan, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L" in Zbornik Matice srpske za prirodne nauke, no. 137 (2019):49,
https://doi.org/10.2298/ZMSPN1937049I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, Dragana
AU  - Ivanović, Žarko
AU  - Karaman, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/541
AB  - During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank.
AB  - Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia
T1  - Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji
EP  - 129
IS  - 3
SP  - 125
VL  - 55
DO  - 10.5937/RatPov1803125I
ER  - 

@article{
author = "Ignjatov, Maja and Milosević, Dragana and Ivanović, Žarko and Karaman, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica",
year = "2018",
abstract = "During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank., Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia, Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji",
pages = "129-125",
number = "3",
volume = "55",
doi = "10.5937/RatPov1803125I"
}

Ignjatov, M., Milosević, D., Ivanović, Ž., Karaman, M., Vlajić, S., Nikolić, Z.,& Gvozdanović-Varga, J.. (2018). Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 55(3), 125-129.
https://doi.org/10.5937/RatPov1803125I

Ignjatov M, Milosević D, Ivanović Ž, Karaman M, Vlajić S, Nikolić Z, Gvozdanović-Varga J. Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo. 2018;55(3):125-129.
doi:10.5937/RatPov1803125I .

Ignjatov, Maja, Milosević, Dragana, Ivanović, Žarko, Karaman, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia" in Ratarstvo i povrtarstvo, 55, no. 3 (2018):125-129,
https://doi.org/10.5937/RatPov1803125I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović Milovanović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1026
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
EP  - 17
SP  - 9
VL  - 132
DO  - 10.2298/ZMSPN1732009I
DO  - 0352-4906
ER  - 

@article{
author = "Ignjatov, Maja and Popović Milovanović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)",
pages = "17-9",
volume = "132",
doi = "10.2298/ZMSPN1732009I, 0352-4906"
}

Ignjatov, M., Popović Milovanović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 132, 9-17.
https://doi.org/10.2298/ZMSPN1732009I

Ignjatov M, Popović Milovanović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;132:9-17.
doi:10.2298/ZMSPN1732009I .

Ignjatov, Maja, Popović Milovanović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/504
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45"
}

Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.

Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596..

Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596.

Vučurović, I., Vučurović, A., Nikolić, D., Bulajić, A., Milošević, D., Krstić, B.,& Stanković, I.. (2016). First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease
American Phytopathological Society., 100(1), 230-231.
https://doi.org/10.1094/PDIS-04-15-0432-PDN

Vučurović I, Vučurović A, Nikolić D, Bulajić A, Milošević D, Krstić B, Stanković I. First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease. 2016;100(1):230-231.
doi:10.1094/PDIS-04-15-0432-PDN .

Vučurović, Ivan, Vučurović, Ana, Nikolić, Dušan, Bulajić, Aleksandra, Milošević, D., Krstić, Branka, Stanković, Ivana, "First Report of Leek yellow stripe virus in Leek in Serbia" in Plant Disease, 100, no. 1 (2016):230-231,
https://doi.org/10.1094/PDIS-04-15-0432-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/460
AB  - During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
AB  - Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
T1  - Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji
EP  - 45
IS  - 2
SP  - 42
VL  - 53
DO  - 10.5937/ratpov53-9984
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Vasić, Mirjana and Nikolić, Zorica and Tamindžić, Gordana and Ivanović, Žarko",
year = "2016",
abstract = "During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot., Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia, Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji",
pages = "45-42",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9984"
}

Ignjatov, M., Popović, T., Milošević, D., Vasić, M., Nikolić, Z., Tamindžić, G.,& Ivanović, Ž.. (2016). Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 53(2), 42-45.
https://doi.org/10.5937/ratpov53-9984

Ignjatov M, Popović T, Milošević D, Vasić M, Nikolić Z, Tamindžić G, Ivanović Ž. Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo. 2016;53(2):42-45.
doi:10.5937/ratpov53-9984 .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Vasić, Mirjana, Nikolić, Zorica, Tamindžić, Gordana, Ivanović, Žarko, "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia" in Ratarstvo i povrtarstvo, 53, no. 2 (2016):42-45,
https://doi.org/10.5937/ratpov53-9984 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1135
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 

@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}

Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769

Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .

Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavost" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/241
AB  - Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri.
AB  - Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Isolation of bacteriophages and their use in Xanthomonas spp. differantiation
T1  - Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.
EP  - 75
IS  - 2
SP  - 62
VL  - 63
ER  - 

@article{
author = "Gašić, Katarina and Ivanović, Milan and Prokić, Anđelka and Kuzmanović, Nemanja and Ignjatov, Maja and Obradović, Aleksa",
year = "2012",
abstract = "Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri., Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation, Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.",
pages = "75-62",
number = "2",
volume = "63"
}

Gašić, K., Ivanović, M., Prokić, A., Kuzmanović, N., Ignjatov, M.,& Obradović, A.. (2012). Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(2), 62-75.

Gašić K, Ivanović M, Prokić A, Kuzmanović N, Ignjatov M, Obradović A. Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja. 2012;63(2):62-75..

Gašić, Katarina, Ivanović, Milan, Prokić, Anđelka, Kuzmanović, Nemanja, Ignjatov, Maja, Obradović, Aleksa, "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation" in Zaštita bilja, 63, no. 2 (2012):62-75.

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/230
AB  - Bean production is threaten by phytopathogenic bacteria causing agents of blights, Xanthomonas axonopodis pv. phaseoli (Xap) and Pseudomonas savastanoi pv. phaseolicola (Psp). Since there is no satisfactory chemical control for the disease, the recommended measures are preventive and include use of healthy seed, crop rotation, deep plowing and use of resistant cultivars. In this work we involved a detection method for isolation of Xap and Psp from bean seed to semi-selective medium Milk Agar Tween (MT). On this medium, Xap formed yellow, mucoid and convex colonies with two hydrolysis zones (less milk and more enlightened), and Psp formed whitish-cream, flat and round colonies. The identification of Xap and Psp was confirmed using the ELISA and PCR. Due to its selectivity, easy preparation and possibility of simultaneous detection of bacteria Xap and Psp, MT medium can be recommended for routine test of seed health for local seed or seed from import.
AB  - Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium
T1  - Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge
EP  - 38
IS  - 1
SP  - 34
VL  - 49
DO  - 10.5937/ratpov49-1126
ER  - 

@article{
author = "Popović, Tatjana and Ignjatov, Maja and Jošić, Dragana and Starović, Mira and Živković, Svetlana and Aleksić, Goran and Trkulja, Nenad",
year = "2012",
abstract = "Bean production is threaten by phytopathogenic bacteria causing agents of blights, Xanthomonas axonopodis pv. phaseoli (Xap) and Pseudomonas savastanoi pv. phaseolicola (Psp). Since there is no satisfactory chemical control for the disease, the recommended measures are preventive and include use of healthy seed, crop rotation, deep plowing and use of resistant cultivars. In this work we involved a detection method for isolation of Xap and Psp from bean seed to semi-selective medium Milk Agar Tween (MT). On this medium, Xap formed yellow, mucoid and convex colonies with two hydrolysis zones (less milk and more enlightened), and Psp formed whitish-cream, flat and round colonies. The identification of Xap and Psp was confirmed using the ELISA and PCR. Due to its selectivity, easy preparation and possibility of simultaneous detection of bacteria Xap and Psp, MT medium can be recommended for routine test of seed health for local seed or seed from import., Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium, Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge",
pages = "38-34",
number = "1",
volume = "49",
doi = "10.5937/ratpov49-1126"
}

Popović, T., Ignjatov, M., Jošić, D., Starović, M., Živković, S., Aleksić, G.,& Trkulja, N.. (2012). Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 49(1), 34-38.
https://doi.org/10.5937/ratpov49-1126

Popović T, Ignjatov M, Jošić D, Starović M, Živković S, Aleksić G, Trkulja N. Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium. in Ratarstvo i povrtarstvo. 2012;49(1):34-38.
doi:10.5937/ratpov49-1126 .

Popović, Tatjana, Ignjatov, Maja, Jošić, Dragana, Starović, Mira, Živković, Svetlana, Aleksić, Goran, Trkulja, Nenad, "Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium" in Ratarstvo i povrtarstvo, 49, no. 1 (2012):34-38,
https://doi.org/10.5937/ratpov49-1126 . .