TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Ivanović, Mirko
AU  - Krstić, Branka
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/870
AB  - Although natural infections of potato in Serbia have not been detected, two TSWV isolates from tobacco, with different biological features were compared based on NSm gene sequence analysis and they were used for mechanical and graft inoculations to evaluate the susceptibility of five popular potato cultivars: 'Riviera', 'Arnova', 'Curoda', 'Kondor' and 'Aladin'. Both TSWV isolates infected all tested potato cultivars. Statistical analyses showed that the rate of TSWV transmission from infected foliage to tubers and from infected tubers to progeny plants was affected by cultivars, while virus transmission efficiency was independent of investigated isolates. Different potato tissues used for sample preparation did not influence virus detection by ELISA, but composite sampling from six different tuber parts is recommended since it was the most reliable.
PB  - Springer
T2  - American Journal of Potato Research
T1  - Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission
EP  - 194
IS  - 2
SP  - 186
VL  - 91
DO  - 10.1007/s12230-013-9337-9
ER  - 

@article{
author = "Bulajić, Aleksandra and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Ivanović, Mirko and Krstić, Branka",
year = "2014",
abstract = "Although natural infections of potato in Serbia have not been detected, two TSWV isolates from tobacco, with different biological features were compared based on NSm gene sequence analysis and they were used for mechanical and graft inoculations to evaluate the susceptibility of five popular potato cultivars: 'Riviera', 'Arnova', 'Curoda', 'Kondor' and 'Aladin'. Both TSWV isolates infected all tested potato cultivars. Statistical analyses showed that the rate of TSWV transmission from infected foliage to tubers and from infected tubers to progeny plants was affected by cultivars, while virus transmission efficiency was independent of investigated isolates. Different potato tissues used for sample preparation did not influence virus detection by ELISA, but composite sampling from six different tuber parts is recommended since it was the most reliable.",
publisher = "Springer",
journal = "American Journal of Potato Research",
title = "Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission",
pages = "194-186",
number = "2",
volume = "91",
doi = "10.1007/s12230-013-9337-9"
}

Bulajić, A., Stanković, I., Vučurović, A., Ristić, D., Milojević, K., Ivanović, M.,& Krstić, B.. (2014). Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission. in American Journal of Potato Research
Springer., 91(2), 186-194.
https://doi.org/10.1007/s12230-013-9337-9

Bulajić A, Stanković I, Vučurović A, Ristić D, Milojević K, Ivanović M, Krstić B. Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission. in American Journal of Potato Research. 2014;91(2):186-194.
doi:10.1007/s12230-013-9337-9 .

Bulajić, Aleksandra, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Ivanović, Mirko, Krstić, Branka, "Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission" in American Journal of Potato Research, 91, no. 2 (2014):186-194,
https://doi.org/10.1007/s12230-013-9337-9 . .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Nikolić, Dušan
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1100
AB  - Proučavanjem pojave i rasprostranjenosti virusa mozaika krastavca u usevu lubenice u Srbiji tokom 2012. godine, prisustvo virusa utvrđeno je u pet od šest pregledanih lokaliteta. Prisustvo virusa ustanovljeno je u 24,05% serološki testiranih uzoraka (DAS-ELISA), a najzastupljeniji je bio na lokalitetu Silbaš. Tokom ovih istraživanja virus mozaika krastavca češće je bio prisutan u mešanim infekcijama sa ZYMV i/ili WMV (12,66%), dok je prisustvo pojedinačne infekcije utvrđeno u nešto nižem procentu, 11,39%. Mehaničkim inokulacijama N. glutinosa, dobijeno je pet izolata, od kojih su dva odabrana za dalju biološku karakterizaciju. Na osnovu tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem para prajmera CMVCPfwd/CPrev, koji omogućavaju umnožavanje gena za protein omotača i deo 5' i 3' UTR regiona. Analizom sekvenci kompletnog gena za protein omotača i rekonstrukcijom filogenetskog stabla, utvrđena je pripadnost izolata CMV iz lubenice iz Srbije podgrupi IA, koja obuhvata većinu izolata odabranih za filogenetsku analizu.
PB  - Institut za zaštitu bilja i životnu sredinu
T2  - Zaštita bilja
T1  - Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji
EP  - 25
IS  - 1
SP  - 14
VL  - 64
ER  - 

@article{
author = "Milojević, Katarina and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Nikolić, Dušan and Bulajić, Aleksandra and Krstić, Branka",
year = "2013",
abstract = "Proučavanjem pojave i rasprostranjenosti virusa mozaika krastavca u usevu lubenice u Srbiji tokom 2012. godine, prisustvo virusa utvrđeno je u pet od šest pregledanih lokaliteta. Prisustvo virusa ustanovljeno je u 24,05% serološki testiranih uzoraka (DAS-ELISA), a najzastupljeniji je bio na lokalitetu Silbaš. Tokom ovih istraživanja virus mozaika krastavca češće je bio prisutan u mešanim infekcijama sa ZYMV i/ili WMV (12,66%), dok je prisustvo pojedinačne infekcije utvrđeno u nešto nižem procentu, 11,39%. Mehaničkim inokulacijama N. glutinosa, dobijeno je pet izolata, od kojih su dva odabrana za dalju biološku karakterizaciju. Na osnovu tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem para prajmera CMVCPfwd/CPrev, koji omogućavaju umnožavanje gena za protein omotača i deo 5' i 3' UTR regiona. Analizom sekvenci kompletnog gena za protein omotača i rekonstrukcijom filogenetskog stabla, utvrđena je pripadnost izolata CMV iz lubenice iz Srbije podgrupi IA, koja obuhvata većinu izolata odabranih za filogenetsku analizu.",
publisher = "Institut za zaštitu bilja i životnu sredinu",
journal = "Zaštita bilja",
title = "Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji",
pages = "25-14",
number = "1",
volume = "64"
}

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Nikolić, D., Bulajić, A.,& Krstić, B.. (2013). Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu., 64(1), 14-25.

Milojević K, Stanković I, Vučurović A, Ristić D, Nikolić D, Bulajić A, Krstić B. Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji. in Zaštita bilja. 2013;64(1):14-25..

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Nikolić, Dušan, Bulajić, Aleksandra, Krstić, Branka, "Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji" in Zaštita bilja, 64, no. 1 (2013):14-25.

TY  - JOUR
AU  - Popović, Milica
AU  - Bulajić, Aleksandra
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Jankov, Ratko
AU  - Gavrilović-Jankulović, Marija
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/933
AB  - Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit
EP  - 3078
IS  - 15
SP  - 3072
VL  - 92
DO  - 10.1002/jsfa.5728
ER  - 

@article{
author = "Popović, Milica and Bulajić, Aleksandra and Ristić, Danijela and Krstić, Branka and Jankov, Ratko and Gavrilović-Jankulović, Marija",
year = "2012",
abstract = "Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit",
pages = "3078-3072",
number = "15",
volume = "92",
doi = "10.1002/jsfa.5728"
}

Popović, M., Bulajić, A., Ristić, D., Krstić, B., Jankov, R.,& Gavrilović-Jankulović, M.. (2012). In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture
Wiley., 92(15), 3072-3078.
https://doi.org/10.1002/jsfa.5728

Popović M, Bulajić A, Ristić D, Krstić B, Jankov R, Gavrilović-Jankulović M. In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture. 2012;92(15):3072-3078.
doi:10.1002/jsfa.5728 .

Popović, Milica, Bulajić, Aleksandra, Ristić, Danijela, Krstić, Branka, Jankov, Ratko, Gavrilović-Jankulović, Marija, "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit" in Journal of the Science of Food and Agriculture, 92, no. 15 (2012):3072-3078,
https://doi.org/10.1002/jsfa.5728 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/901
AB  - Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5' and 3' UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Characterization of cucumber mosaic virus originating from cucurbits in Serbia
EP  - 336
IS  - 4
SP  - 325
VL  - 26
DO  - 10.2298/PIF1104325V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5' and 3' UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Characterization of cucumber mosaic virus originating from cucurbits in Serbia",
pages = "336-325",
number = "4",
volume = "26",
doi = "10.2298/PIF1104325V"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2011). Characterization of cucumber mosaic virus originating from cucurbits in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 26(4), 325-336.
https://doi.org/10.2298/PIF1104325V

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Krstić B. Characterization of cucumber mosaic virus originating from cucurbits in Serbia. in Pesticidi i fitomedicina. 2011;26(4):325-336.
doi:10.2298/PIF1104325V .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Characterization of cucumber mosaic virus originating from cucurbits in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):325-336,
https://doi.org/10.2298/PIF1104325V . .