TY  - JOUR
AU  - Toševski, Ivo
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Gassmann, Andre
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/295
AB  - Several populations of the stem-mining weevil Mecinus janthinus Germar species complex (Mecinini, Curculionidae), identified based on morphological characteristics, have been introduced in North America for the biological control of invasive toadflaxes of European origin: Linaria vulgaris Miller and L. dalmatica (L.) Miller (Plantaginaceae). According to the mitochondrial cytochrome oxidase subunit II (COII) gene haplotype divergence of Mecinus janthinus species complex, a total of 20 M. janthinus s.s., 3 M. janthinus s.l. of the 'speciosa' genotype and 29 M. janthiniformis haplotypes have been recorded across their native range in central and southeastern Europe. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) diagnostic assay of COII gene using Hpy188III and MnlI enzyme-mix, was developed for fast and cost-effective discrimination of these morphologically very similar cryptic weevil species. It is shown that digestion generates unique 4-fragment restriction profile in M. janthinus s.s., 2-fragment profile in M. janthiniformis and 3-fragment profile in M. janthinus s.l. 'speciosa' group of haplotypes, allowing precise identification of each species or genotype. The proposed method represents a practical tool for fast and accurate identification of the target biocontrol agents and should prevent using inappropriate weevil species in redistribution programs for biological control of invasive toadflax species.
PB  - Springer, Dordrecht
T2  - BioControl
T1  - PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes
EP  - 573
IS  - 4
SP  - 563
VL  - 58
DO  - 10.1007/s10526-013-9506-2
ER  - 

@article{
author = "Toševski, Ivo and Jović, Jelena and Krstić, Oliver and Gassmann, Andre",
year = "2013",
abstract = "Several populations of the stem-mining weevil Mecinus janthinus Germar species complex (Mecinini, Curculionidae), identified based on morphological characteristics, have been introduced in North America for the biological control of invasive toadflaxes of European origin: Linaria vulgaris Miller and L. dalmatica (L.) Miller (Plantaginaceae). According to the mitochondrial cytochrome oxidase subunit II (COII) gene haplotype divergence of Mecinus janthinus species complex, a total of 20 M. janthinus s.s., 3 M. janthinus s.l. of the 'speciosa' genotype and 29 M. janthiniformis haplotypes have been recorded across their native range in central and southeastern Europe. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) diagnostic assay of COII gene using Hpy188III and MnlI enzyme-mix, was developed for fast and cost-effective discrimination of these morphologically very similar cryptic weevil species. It is shown that digestion generates unique 4-fragment restriction profile in M. janthinus s.s., 2-fragment profile in M. janthiniformis and 3-fragment profile in M. janthinus s.l. 'speciosa' group of haplotypes, allowing precise identification of each species or genotype. The proposed method represents a practical tool for fast and accurate identification of the target biocontrol agents and should prevent using inappropriate weevil species in redistribution programs for biological control of invasive toadflax species.",
publisher = "Springer, Dordrecht",
journal = "BioControl",
title = "PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes",
pages = "573-563",
number = "4",
volume = "58",
doi = "10.1007/s10526-013-9506-2"
}

Toševski, I., Jović, J., Krstić, O.,& Gassmann, A.. (2013). PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes. in BioControl
Springer, Dordrecht., 58(4), 563-573.
https://doi.org/10.1007/s10526-013-9506-2

Toševski I, Jović J, Krstić O, Gassmann A. PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes. in BioControl. 2013;58(4):563-573.
doi:10.1007/s10526-013-9506-2 .

Toševski, Ivo, Jović, Jelena, Krstić, Oliver, Gassmann, Andre, "PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes" in BioControl, 58, no. 4 (2013):563-573,
https://doi.org/10.1007/s10526-013-9506-2 . .

TY  - JOUR
AU  - Toševski, Ivo
AU  - Caldara, Roberto
AU  - Jović, Jelena
AU  - Hernandez-Vera, Gerardo
AU  - Baviera, Cosimo
AU  - Gassmann, Andre
AU  - Emerson, Brent C.
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/152
AB  - A combined morphological, molecular and biological study shows that the weevil species presently named Mecinus janthinus is actually composed of two different cryptic species: M. janthinus Germar, 1821 and M. janthiniformis Tosevski & Caldara sp.n. These species are morphologically distinguishable from each other by a few very subtle morphological characters. On the contrary, they are more readily distinguishable by both molecular and biological characters. A molecular assessment based on the mitochondrial DNA cytochrome oxidase subunit II gene revealed fixed differences between the two species with p-distances between samples of both species ranging from 1.3 to 2.4%. In addition to this, the larvae of the two species are found to develop on different species within the genus Linaria (Plantaginaceae): M. janthinus is associated with yellow toadflax (L. vulgaris) and M. janthiniformis with broomleaf toadflax (L. genistifolia) and Dalmatian toadflax (L. dalmatica). Molecular and host use records further suggest the occurrence of a third species associated with L. vulgaris within M. janthinus, sampled from north Switzerland, central Hungary and east Serbia. The significance of these new findings is of particular importance because species of the M. janthinus group are used, or are potential candidates, for the biological control of invasive toadflaxes in North America.
PB  - Wiley-Blackwell, Hoboken
T2  - Systematic Entomology
T1  - Morphological, molecular and biological evidence reveal two cryptic species in Mecinus janthinus Germar (Coleoptera, Curculionidae), a successful biological control agent of Dalmatian toadflax, Linaria dalmatica (Lamiales, Plantaginaceae)
EP  - 753
IS  - 4
SP  - 741
VL  - 36
DO  - 10.1111/j.1365-3113.2011.00593.x
ER  - 

@article{
author = "Toševski, Ivo and Caldara, Roberto and Jović, Jelena and Hernandez-Vera, Gerardo and Baviera, Cosimo and Gassmann, Andre and Emerson, Brent C.",
year = "2011",
abstract = "A combined morphological, molecular and biological study shows that the weevil species presently named Mecinus janthinus is actually composed of two different cryptic species: M. janthinus Germar, 1821 and M. janthiniformis Tosevski & Caldara sp.n. These species are morphologically distinguishable from each other by a few very subtle morphological characters. On the contrary, they are more readily distinguishable by both molecular and biological characters. A molecular assessment based on the mitochondrial DNA cytochrome oxidase subunit II gene revealed fixed differences between the two species with p-distances between samples of both species ranging from 1.3 to 2.4%. In addition to this, the larvae of the two species are found to develop on different species within the genus Linaria (Plantaginaceae): M. janthinus is associated with yellow toadflax (L. vulgaris) and M. janthiniformis with broomleaf toadflax (L. genistifolia) and Dalmatian toadflax (L. dalmatica). Molecular and host use records further suggest the occurrence of a third species associated with L. vulgaris within M. janthinus, sampled from north Switzerland, central Hungary and east Serbia. The significance of these new findings is of particular importance because species of the M. janthinus group are used, or are potential candidates, for the biological control of invasive toadflaxes in North America.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Systematic Entomology",
title = "Morphological, molecular and biological evidence reveal two cryptic species in Mecinus janthinus Germar (Coleoptera, Curculionidae), a successful biological control agent of Dalmatian toadflax, Linaria dalmatica (Lamiales, Plantaginaceae)",
pages = "753-741",
number = "4",
volume = "36",
doi = "10.1111/j.1365-3113.2011.00593.x"
}

Toševski, I., Caldara, R., Jović, J., Hernandez-Vera, G., Baviera, C., Gassmann, A.,& Emerson, B. C.. (2011). Morphological, molecular and biological evidence reveal two cryptic species in Mecinus janthinus Germar (Coleoptera, Curculionidae), a successful biological control agent of Dalmatian toadflax, Linaria dalmatica (Lamiales, Plantaginaceae). in Systematic Entomology
Wiley-Blackwell, Hoboken., 36(4), 741-753.
https://doi.org/10.1111/j.1365-3113.2011.00593.x

Toševski I, Caldara R, Jović J, Hernandez-Vera G, Baviera C, Gassmann A, Emerson BC. Morphological, molecular and biological evidence reveal two cryptic species in Mecinus janthinus Germar (Coleoptera, Curculionidae), a successful biological control agent of Dalmatian toadflax, Linaria dalmatica (Lamiales, Plantaginaceae). in Systematic Entomology. 2011;36(4):741-753.
doi:10.1111/j.1365-3113.2011.00593.x .

Toševski, Ivo, Caldara, Roberto, Jović, Jelena, Hernandez-Vera, Gerardo, Baviera, Cosimo, Gassmann, Andre, Emerson, Brent C., "Morphological, molecular and biological evidence reveal two cryptic species in Mecinus janthinus Germar (Coleoptera, Curculionidae), a successful biological control agent of Dalmatian toadflax, Linaria dalmatica (Lamiales, Plantaginaceae)" in Systematic Entomology, 36, no. 4 (2011):741-753,
https://doi.org/10.1111/j.1365-3113.2011.00593.x . .