TY  - JOUR
AU  - Savković, Željko
AU  - Stupar, Milos
AU  - Unković, Nikola
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Popović, Slađana
AU  - Vukojevic, Jelena
AU  - Ljaljević-Grbić, Milica
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/748
AB  - An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.
PB  - Elsevier
T2  - International Biodeterioration & Biodegradation
T1  - Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility
EP  - 163
SP  - 105
VL  - 157
DO  - 10.1016/j.ibiod.2020.105163
ER  - 

@article{
author = "Savković, Željko and Stupar, Milos and Unković, Nikola and Ivanović, Žarko and Blagojević, Jovana and Popović, Slađana and Vukojevic, Jelena and Ljaljević-Grbić, Milica",
year = "2021",
abstract = "An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.",
publisher = "Elsevier",
journal = "International Biodeterioration & Biodegradation",
title = "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility",
pages = "163-105",
volume = "157",
doi = "10.1016/j.ibiod.2020.105163"
}

Savković, Ž., Stupar, M., Unković, N., Ivanović, Ž., Blagojević, J., Popović, S., Vukojevic, J.,& Ljaljević-Grbić, M.. (2021). Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation
Elsevier., 157, 105-163.
https://doi.org/10.1016/j.ibiod.2020.105163

Savković Ž, Stupar M, Unković N, Ivanović Ž, Blagojević J, Popović S, Vukojevic J, Ljaljević-Grbić M. Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation. 2021;157:105-163.
doi:10.1016/j.ibiod.2020.105163 .

Savković, Željko, Stupar, Milos, Unković, Nikola, Ivanović, Žarko, Blagojević, Jovana, Popović, Slađana, Vukojevic, Jelena, Ljaljević-Grbić, Milica, "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility" in International Biodeterioration & Biodegradation, 157 (2021):105-163,
https://doi.org/10.1016/j.ibiod.2020.105163 . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Gašić, Katarina
AU  - Starović, Mira
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/717
AB  - Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia
EP  - 667
IS  - 3
SP  - 656
VL  - 104
DO  - 10.1094/PDIS-08-19-1806-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Gašić, Katarina and Starović, Mira and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2020",
abstract = "Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia",
pages = "667-656",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1806-RE"
}

Stošić, S., Ristić, L., Gašić, K., Starović, M., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2020). Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease
The American Phytopathological Society., 104(3), 656-667.
https://doi.org/10.1094/PDIS-08-19-1806-RE

Stošić S, Ristić L, Gašić K, Starović M, Ljaljevic Grbic M, Vukojevic J, Živković S. Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease. 2020;104(3):656-667.
doi:10.1094/PDIS-08-19-1806-RE .

Stošić, Stefan, Ristić, Lela, Gašić, Katarina, Starović, Mira, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia" in Plant Disease, 104, no. 3 (2020):656-667,
https://doi.org/10.1094/PDIS-08-19-1806-RE . .

TY  - JOUR
AU  - Savković, Zeljko
AU  - Stupar, Milos
AU  - Unković, Nikola
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Vukojević, Jelena
AU  - Ljaljević-Grbić, Milica
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/568
AB  - The study addresses in vitro degradation potential of airborne Aspergillus and Penicillium/Talarmyces species originating from cultural heritage conservation premises. A series of rapid, cost effective biodegradation assays were performed to assess production of extracellular pigments, acids, and enzymes. Most of the isolates have demonstrated positive growth in at least one of the preformed tests. Strongest overall degradation potential was demonstrated for Penicillium brevicompactum, P. glabrum, and Talaromyces sayulitensis while Aspergillus domesticus, A. penicillioides, A. pseudoglaucus, and A. ruber did not exhibit positive reaction in any of the employed assays. Majority of isolates exhibited proteolytic and cellulolytic activity while carbonate dissolution was observed for only five tested fungi. Highest alteration of pH value in liquid media was documented for T. sayulitensis while A. niger and P. expansum exhibited strongest acid production on CREA. Certain isolates, mostly Penicillium species, displayed production of extracellular pigments. The results imply that many of the tested fungi have significant biodegradation capacity, indicating their potential to inflict structural and esthetic alterations on cultural heritage objects.
PB  - Springer Heidelberg, Heidelberg
T2  - Science of Nature
T1  - In vitro biodegradation potential of airborne Aspergilli and Penicillia
IS  - 3-4
VL  - 106
DO  - 10.1007/s00114-019-1603-3
ER  - 

@article{
author = "Savković, Zeljko and Stupar, Milos and Unković, Nikola and Ivanović, Žarko and Blagojević, Jovana and Vukojević, Jelena and Ljaljević-Grbić, Milica",
year = "2019",
abstract = "The study addresses in vitro degradation potential of airborne Aspergillus and Penicillium/Talarmyces species originating from cultural heritage conservation premises. A series of rapid, cost effective biodegradation assays were performed to assess production of extracellular pigments, acids, and enzymes. Most of the isolates have demonstrated positive growth in at least one of the preformed tests. Strongest overall degradation potential was demonstrated for Penicillium brevicompactum, P. glabrum, and Talaromyces sayulitensis while Aspergillus domesticus, A. penicillioides, A. pseudoglaucus, and A. ruber did not exhibit positive reaction in any of the employed assays. Majority of isolates exhibited proteolytic and cellulolytic activity while carbonate dissolution was observed for only five tested fungi. Highest alteration of pH value in liquid media was documented for T. sayulitensis while A. niger and P. expansum exhibited strongest acid production on CREA. Certain isolates, mostly Penicillium species, displayed production of extracellular pigments. The results imply that many of the tested fungi have significant biodegradation capacity, indicating their potential to inflict structural and esthetic alterations on cultural heritage objects.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Science of Nature",
title = "In vitro biodegradation potential of airborne Aspergilli and Penicillia",
number = "3-4",
volume = "106",
doi = "10.1007/s00114-019-1603-3"
}

Savković, Z., Stupar, M., Unković, N., Ivanović, Ž., Blagojević, J., Vukojević, J.,& Ljaljević-Grbić, M.. (2019). In vitro biodegradation potential of airborne Aspergilli and Penicillia. in Science of Nature
Springer Heidelberg, Heidelberg., 106(3-4).
https://doi.org/10.1007/s00114-019-1603-3

Savković Z, Stupar M, Unković N, Ivanović Ž, Blagojević J, Vukojević J, Ljaljević-Grbić M. In vitro biodegradation potential of airborne Aspergilli and Penicillia. in Science of Nature. 2019;106(3-4).
doi:10.1007/s00114-019-1603-3 .

Savković, Zeljko, Stupar, Milos, Unković, Nikola, Ivanović, Žarko, Blagojević, Jovana, Vukojević, Jelena, Ljaljević-Grbić, Milica, "In vitro biodegradation potential of airborne Aspergilli and Penicillia" in Science of Nature, 106, no. 3-4 (2019),
https://doi.org/10.1007/s00114-019-1603-3 . .

TY  - JOUR
AU  - Stajić, Mirjana
AU  - Ćilerdžić, Jasmina Lj.
AU  - Galić, Milica
AU  - Ivanović, Žarko
AU  - Vukojević, Jelena
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/472
AB  - The properties and capacities of the ligninolytic enzymes of Daedaleopsis spp. are still unknown. This is the first study on the effect of plant residues and period of cultivation on the properties of Mn-oxidizing peroxidases and laccases of D. confragosa and D. tricolor, as well as their ligninolytic potentials. Wheat straw was the optimal carbon source for synthesis of highly active Mn-dependent peroxidases (4126.9 U/L in D. confragosa and 2037.9 U/L in D. tricolor). However, laccases were the predominant enzymes, and the best inducer of their activity (up 16000.0 U/L) was cherry sawdust. Wheat straw was the most susceptible plant residue to the effect of the enzymes, and extent of lignin degradation was 43.3% after 14 days of fermentation with D. tricolor. However, D. confragosa was a more effective lignin degrader, as it converted even 21.3% wheat straw lignin on the 6th day of cultivation. The results of the study clearly showed that delignification extent depends on mushroom species and on the type of plant residue, which is extremely important for potential use in biotechnological processes.
PB  - North Carolina State University
T2  - Bioresources
T1  - Lignocellulose Degradation by Daedaleopsis confragosa and D-tricolor
EP  - 7204
IS  - 4
SP  - 7195
VL  - 12
DO  - 10.15376/biores.12.4.7195-7204
ER  - 

@article{
author = "Stajić, Mirjana and Ćilerdžić, Jasmina Lj. and Galić, Milica and Ivanović, Žarko and Vukojević, Jelena",
year = "2017",
abstract = "The properties and capacities of the ligninolytic enzymes of Daedaleopsis spp. are still unknown. This is the first study on the effect of plant residues and period of cultivation on the properties of Mn-oxidizing peroxidases and laccases of D. confragosa and D. tricolor, as well as their ligninolytic potentials. Wheat straw was the optimal carbon source for synthesis of highly active Mn-dependent peroxidases (4126.9 U/L in D. confragosa and 2037.9 U/L in D. tricolor). However, laccases were the predominant enzymes, and the best inducer of their activity (up 16000.0 U/L) was cherry sawdust. Wheat straw was the most susceptible plant residue to the effect of the enzymes, and extent of lignin degradation was 43.3% after 14 days of fermentation with D. tricolor. However, D. confragosa was a more effective lignin degrader, as it converted even 21.3% wheat straw lignin on the 6th day of cultivation. The results of the study clearly showed that delignification extent depends on mushroom species and on the type of plant residue, which is extremely important for potential use in biotechnological processes.",
publisher = "North Carolina State University",
journal = "Bioresources",
title = "Lignocellulose Degradation by Daedaleopsis confragosa and D-tricolor",
pages = "7204-7195",
number = "4",
volume = "12",
doi = "10.15376/biores.12.4.7195-7204"
}

Stajić, M., Ćilerdžić, J. Lj., Galić, M., Ivanović, Ž.,& Vukojević, J.. (2017). Lignocellulose Degradation by Daedaleopsis confragosa and D-tricolor. in Bioresources
North Carolina State University., 12(4), 7195-7204.
https://doi.org/10.15376/biores.12.4.7195-7204

Stajić M, Ćilerdžić JL, Galić M, Ivanović Ž, Vukojević J. Lignocellulose Degradation by Daedaleopsis confragosa and D-tricolor. in Bioresources. 2017;12(4):7195-7204.
doi:10.15376/biores.12.4.7195-7204 .

Stajić, Mirjana, Ćilerdžić, Jasmina Lj., Galić, Milica, Ivanović, Žarko, Vukojević, Jelena, "Lignocellulose Degradation by Daedaleopsis confragosa and D-tricolor" in Bioresources, 12, no. 4 (2017):7195-7204,
https://doi.org/10.15376/biores.12.4.7195-7204 . .