TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Stojanović, Milan
AU  - Popović, Vera
AU  - Ivanović, Žarko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/662
AB  - Fusarium spp. are important soil- and seed-borne pathogens of many field and vegetable crops, including orchards and medicinal plants (Leslie and Summerell 2006). Fusarium proliferatum is the most common pathogen infecting numerous crop plants and occurring in various climatic zones. In Serbia, this species is well-known as a pathogen of wheat, maize, bean, and recently, garlic and onion (Lević et al. 2009; Stanković et al. 2007). Hyssop (Hyssopus officinalis L.) is grown in Serbia as a member of the Lamiaceae family for the needs of pharmaceutical companies and tea production, because of its medicinal and aromatic properties. In Serbia, production takes place on about 500 ha (no official data). During a routine quality control of hyssop seeds collected from Rumenka (Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an average of 22%. White mycelium covered infected seeds with violet pigmentation occurring under the seeds. Microscopic observation confirmed the presence of Fusarium spp. Prior to isolation, seeds were surface disinfected in 1% NaOCl for 3 min, rinsed, dried, and plated onto potato dextrose agar (PDA). Plates were incubated at 25°C under ultraviolet light (“black light”) with a 12-h photoperiod (Mathur and Kongsdall 2003). Seven days later, 12 Fusarium spp. isolates (JBL 4003/1 to 4003/12) were single spored and subcultured on both PDA and carnation leaf agar. Pathogenicity testing was performed in vitro using a modified agar slant method in the test tube with PDA amended (Porter et al. 2015). A piece of mycelium of each isolate grown on PDA for 7 days was placed at the bottom of the tube, and 2 cm above the inoculum, dried hyssop seed was carefully placed. After 10 days, fungal mycelia of 12 isolates caused seed rot and seedling decay. All the tested isolates were reisolated and used for further analysis. The isolate JBL 4003/2 formed white, aerial, and abundant colonies, with light violet to brown pigmentation in agar. Within 5 days, microconidia were formed in the aerial mycelium, in long chains or cohering in false heads. Slightly curved rather straight macroconidia were formed, with a distinct foot cell, mostly three to five septate, with average dimensions of 31 to 53 × 3.4 to 4.1 µm. No chlamydospores were observed. Based on the description given by Gerlach and Nirenberg (1982), cultural and morphological characteristics indicated that the isolate JBL 4003/2 belongs to F. proliferatum. To obtain a DNA sequence-based identification, total DNA was extracted directly from the mycelium with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2 (Geiser et al. 2004). Sequences were analyzed and BLAST searched against GenBank (http://blast.ncbi.nlm.nih.gov/) and FUSARIUM-ID (http://fusariumdb.org/). Isolate designated as JBL 4003/2 was deposited in the NCBI GenBank database under the accession number MK061541.1. BLASTn queries of GenBank and the Fusarium-ID database showed 100% identity to accession numbers KY801934.1 and MK507798.1, which belong to F. proliferatum (Matsushima) Nirenberg. Based on Koch’s postulates and sequence analysis, to our knowledge this is the first report of F. proliferatum as the causal agent of H. officinalis seed rot in Serbia.
T2  - Plant Disease
T1  - First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia
EP  - 1864
IS  - 6
SP  - 1864
VL  - 104
DO  - 10.1094/PDIS-10-19-2218-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Stojanović, Milan and Popović, Vera and Ivanović, Žarko",
year = "2020",
abstract = "Fusarium spp. are important soil- and seed-borne pathogens of many field and vegetable crops, including orchards and medicinal plants (Leslie and Summerell 2006). Fusarium proliferatum is the most common pathogen infecting numerous crop plants and occurring in various climatic zones. In Serbia, this species is well-known as a pathogen of wheat, maize, bean, and recently, garlic and onion (Lević et al. 2009; Stanković et al. 2007). Hyssop (Hyssopus officinalis L.) is grown in Serbia as a member of the Lamiaceae family for the needs of pharmaceutical companies and tea production, because of its medicinal and aromatic properties. In Serbia, production takes place on about 500 ha (no official data). During a routine quality control of hyssop seeds collected from Rumenka (Vojvodina Province), in 2018, fungal infection followed by seed rot was noticed on an average of 22%. White mycelium covered infected seeds with violet pigmentation occurring under the seeds. Microscopic observation confirmed the presence of Fusarium spp. Prior to isolation, seeds were surface disinfected in 1% NaOCl for 3 min, rinsed, dried, and plated onto potato dextrose agar (PDA). Plates were incubated at 25°C under ultraviolet light (“black light”) with a 12-h photoperiod (Mathur and Kongsdall 2003). Seven days later, 12 Fusarium spp. isolates (JBL 4003/1 to 4003/12) were single spored and subcultured on both PDA and carnation leaf agar. Pathogenicity testing was performed in vitro using a modified agar slant method in the test tube with PDA amended (Porter et al. 2015). A piece of mycelium of each isolate grown on PDA for 7 days was placed at the bottom of the tube, and 2 cm above the inoculum, dried hyssop seed was carefully placed. After 10 days, fungal mycelia of 12 isolates caused seed rot and seedling decay. All the tested isolates were reisolated and used for further analysis. The isolate JBL 4003/2 formed white, aerial, and abundant colonies, with light violet to brown pigmentation in agar. Within 5 days, microconidia were formed in the aerial mycelium, in long chains or cohering in false heads. Slightly curved rather straight macroconidia were formed, with a distinct foot cell, mostly three to five septate, with average dimensions of 31 to 53 × 3.4 to 4.1 µm. No chlamydospores were observed. Based on the description given by Gerlach and Nirenberg (1982), cultural and morphological characteristics indicated that the isolate JBL 4003/2 belongs to F. proliferatum. To obtain a DNA sequence-based identification, total DNA was extracted directly from the mycelium with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR using the primer pair EF1 and EF2 (Geiser et al. 2004). Sequences were analyzed and BLAST searched against GenBank (http://blast.ncbi.nlm.nih.gov/) and FUSARIUM-ID (http://fusariumdb.org/). Isolate designated as JBL 4003/2 was deposited in the NCBI GenBank database under the accession number MK061541.1. BLASTn queries of GenBank and the Fusarium-ID database showed 100% identity to accession numbers KY801934.1 and MK507798.1, which belong to F. proliferatum (Matsushima) Nirenberg. Based on Koch’s postulates and sequence analysis, to our knowledge this is the first report of F. proliferatum as the causal agent of H. officinalis seed rot in Serbia.",
journal = "Plant Disease",
title = "First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia",
pages = "1864-1864",
number = "6",
volume = "104",
doi = "10.1094/PDIS-10-19-2218-PDN"
}

Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Stojanović, M., Popović, V.,& Ivanović, Ž.. (2020). First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease, 104(6), 1864-1864.
https://doi.org/10.1094/PDIS-10-19-2218-PDN

Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Stojanović M, Popović V, Ivanović Ž. First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia. in Plant Disease. 2020;104(6):1864-1864.
doi:10.1094/PDIS-10-19-2218-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Stojanović, Milan, Popović, Vera, Ivanović, Žarko, "First Report of Fusarium proliferatum as the Causal Agent of Seed Rot of Hyssopus officinalis in Serbia" in Plant Disease, 104, no. 6 (2020):1864-1864,
https://doi.org/10.1094/PDIS-10-19-2218-PDN . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanovic Jeromela, Ana
AU  - Karaman, Maja
AU  - Grahovac, Mila
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/661
AB  - Matthiola longipetala (Vent) DC, commonly known as “night-scented stock” or “evening stock”, is the most widely cultivated species of the genus Matthiola in the family Brassicaceae. It is a common garden flower, available in a variety of colors, many of which are heavily scented and also used in floristry. An elevated incidence of Fusarium was observed during a routine quality control seed assay of M. longipetala obtained from a private production facility in Đurđevo (South Bačka District) in 2018. Fungal infection was noticed on an average of 30% of the tested seed, followed by a reduction in germination. The infected seed was covered with white to beige mycelium. Prior to isolation, seeds were surface sterilized for 10 min with a 1% sodium hypochlorite solution to reduce contaminants, washed twice in sterile water, and plated on potato dextrose agar (PDA). After 7 days of incubation at 25°C under a 12-h/12-h photoperiod of fluorescent light, 14 Fusarium (JBL4089/1 to 14) isolates were single spored and subcultured on both PDA and carnation leaf agar (CLA). Pathogenicity was performed in vitro using a modified PDA slant method in a test tube (Porter et al. 2015). A piece of mycelium of each isolate grown on PDA for 7 days was placed at the bottom of each tube, and dried M. longipetala seed was carefully placed 2 cm above the inoculum. After 10 days, fungal mycelia of 14 isolates completely covered the seedlings, causing seed rot and seedling decay. The Fusarium was reisolated on PDA and used for further analysis in order to morphologically identify the species. Isolate JBL4089/2 formed abundant, loosely floccose, whitish aerial mycelium with beige pigmentation. After transfer to CLA, the isolate formed macroconidia with a tapered and elongated apical cell and prominent foot-shaped basal cell, which were typically four to five septate, with average dimensions of 21 to 60 × 2.8 to 4.6 µm. The isolate formed chlamydospores, but microconidia were not observed. Based on the morphological characteristics, isolate JBL4089/2 was identified as Fusarium equiseti (Corda) Sacc. according to Leslie and Summerell (2006) and Gerlach and Nirenberg (1982). Identification of isolate JBL4089/2 was confirmed by amplification and sequencing of a portion of the translation elongation factor-1 alpha (EF-1α) gene. Total DNA was extracted directly from fungal mycelium with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany), and PCR amplification was performed with primer pair EF-1/EF-2 (O’Donnell et al. 1998). Sequence analysis of the EF-1α gene revealed 100% nucleotide identity of isolate JBL4089/2 (GenBank accession no. MK061538) with the EF-1α sequences of two F. equiseti isolates from Canada (KU587617 from Pisum sativum and MH315936 from Glycine max) and a Hyssopus officinalis isolate (MK061540) of F. equiseti from Serbia. To our knowledge, this is the first report of F. equiseti as a causal agent of seed rot on M. longipetala in Serbia. The presence of the pathogen could cause significant economic losses in M. longipetala production, and for that reason control strategies for the management of the disease should be implemented.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia
EP  - 2516
IS  - 9
SP  - 2516
VL  - 104
DO  - 10.1094/PDIS-03-20-0602-PDN
ER  - 

@article{
author = "Ivanović, Žarko and Milošević, Dragana and Ignjatov, Maja and Marjanovic Jeromela, Ana and Karaman, Maja and Grahovac, Mila",
year = "2020",
abstract = "Matthiola longipetala (Vent) DC, commonly known as “night-scented stock” or “evening stock”, is the most widely cultivated species of the genus Matthiola in the family Brassicaceae. It is a common garden flower, available in a variety of colors, many of which are heavily scented and also used in floristry. An elevated incidence of Fusarium was observed during a routine quality control seed assay of M. longipetala obtained from a private production facility in Đurđevo (South Bačka District) in 2018. Fungal infection was noticed on an average of 30% of the tested seed, followed by a reduction in germination. The infected seed was covered with white to beige mycelium. Prior to isolation, seeds were surface sterilized for 10 min with a 1% sodium hypochlorite solution to reduce contaminants, washed twice in sterile water, and plated on potato dextrose agar (PDA). After 7 days of incubation at 25°C under a 12-h/12-h photoperiod of fluorescent light, 14 Fusarium (JBL4089/1 to 14) isolates were single spored and subcultured on both PDA and carnation leaf agar (CLA). Pathogenicity was performed in vitro using a modified PDA slant method in a test tube (Porter et al. 2015). A piece of mycelium of each isolate grown on PDA for 7 days was placed at the bottom of each tube, and dried M. longipetala seed was carefully placed 2 cm above the inoculum. After 10 days, fungal mycelia of 14 isolates completely covered the seedlings, causing seed rot and seedling decay. The Fusarium was reisolated on PDA and used for further analysis in order to morphologically identify the species. Isolate JBL4089/2 formed abundant, loosely floccose, whitish aerial mycelium with beige pigmentation. After transfer to CLA, the isolate formed macroconidia with a tapered and elongated apical cell and prominent foot-shaped basal cell, which were typically four to five septate, with average dimensions of 21 to 60 × 2.8 to 4.6 µm. The isolate formed chlamydospores, but microconidia were not observed. Based on the morphological characteristics, isolate JBL4089/2 was identified as Fusarium equiseti (Corda) Sacc. according to Leslie and Summerell (2006) and Gerlach and Nirenberg (1982). Identification of isolate JBL4089/2 was confirmed by amplification and sequencing of a portion of the translation elongation factor-1 alpha (EF-1α) gene. Total DNA was extracted directly from fungal mycelium with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany), and PCR amplification was performed with primer pair EF-1/EF-2 (O’Donnell et al. 1998). Sequence analysis of the EF-1α gene revealed 100% nucleotide identity of isolate JBL4089/2 (GenBank accession no. MK061538) with the EF-1α sequences of two F. equiseti isolates from Canada (KU587617 from Pisum sativum and MH315936 from Glycine max) and a Hyssopus officinalis isolate (MK061540) of F. equiseti from Serbia. To our knowledge, this is the first report of F. equiseti as a causal agent of seed rot on M. longipetala in Serbia. The presence of the pathogen could cause significant economic losses in M. longipetala production, and for that reason control strategies for the management of the disease should be implemented.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia",
pages = "2516-2516",
number = "9",
volume = "104",
doi = "10.1094/PDIS-03-20-0602-PDN"
}

Ivanović, Ž., Milošević, D., Ignjatov, M., Marjanovic Jeromela, A., Karaman, M.,& Grahovac, M.. (2020). First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease
American Phytopathological Society., 104(9), 2516-2516.
https://doi.org/10.1094/PDIS-03-20-0602-PDN

Ivanović Ž, Milošević D, Ignjatov M, Marjanovic Jeromela A, Karaman M, Grahovac M. First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease. 2020;104(9):2516-2516.
doi:10.1094/PDIS-03-20-0602-PDN .

Ivanović, Žarko, Milošević, Dragana, Ignjatov, Maja, Marjanovic Jeromela, Ana, Karaman, Maja, Grahovac, Mila, "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia" in Plant Disease, 104, no. 9 (2020):2516-2516,
https://doi.org/10.1094/PDIS-03-20-0602-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/608
AB  - Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.
AB  - Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L
T1  - Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L
IS  - 137
SP  - 49
DO  - 10.2298/ZMSPN1937049I
ER  - 

@article{
author = "Ignjatov, Maja and Vlajić, Slobodan and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2019",
abstract = "Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identification, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1a gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is "elephant garlic" Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves., Simptomi truleži uskladištenih čenova belog luka na vrsti poznatoj kao "elephant garlic" (A. ampeloprasum) zapaženi su tokom 2018. godine. Prikupljen je veliki broj zaraženih uzoraka i nakon izolacije odabrano je 16 izolata (AMP1-AMP16) za dalja istraživanja. Na osnovu morfoloških i odgajivačkih karakteristika je ustanovljeno da je prouzrokovač truleži čenova gljiva F. proliferatum (telemorf Gibberella intermedia), koja pripada kompleksu Gibberella fujikuroi. Proučavanjem patogenosti svi izolati su prouzrokovali simptome truleži na različitim vrstama roda Allium spp. koji su identični prirodnoj infekciji. U cilju potvrde morfoloških odlika izvršena je molekularna identifikacija metodom lančane reakcije polimeraze (PCR) korišćenjem para prajmera EF1 i EF2 koji amplifikuju TEF-1a gen, sekvencioniranje DNK i filogenetska analiza dobijenih sekvenci. Dobijeni rezultati potvrdili su da je Fusarium proliferatum prouzrokovač truleži na vrsti A. ampeloprasum kao novom domaćinu.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L, Identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži luka Allium ampeloprasum L",
number = "137",
pages = "49",
doi = "10.2298/ZMSPN1937049I"
}

Ignjatov, M., Vlajić, S., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2019). Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(137), 49.
https://doi.org/10.2298/ZMSPN1937049I

Ignjatov M, Vlajić S, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L. in Zbornik Matice srpske za prirodne nauke. 2019;(137):49.
doi:10.2298/ZMSPN1937049I .

Ignjatov, Maja, Vlajić, Slobodan, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L" in Zbornik Matice srpske za prirodne nauke, no. 137 (2019):49,
https://doi.org/10.2298/ZMSPN1937049I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milosević, Dragana
AU  - Ivanović, Žarko
AU  - Karaman, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/541
AB  - During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank.
AB  - Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia
T1  - Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji
EP  - 129
IS  - 3
SP  - 125
VL  - 55
DO  - 10.5937/RatPov1803125I
ER  - 

@article{
author = "Ignjatov, Maja and Milosević, Dragana and Ivanović, Žarko and Karaman, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica",
year = "2018",
abstract = "During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank., Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia, Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji",
pages = "129-125",
number = "3",
volume = "55",
doi = "10.5937/RatPov1803125I"
}

Ignjatov, M., Milosević, D., Ivanović, Ž., Karaman, M., Vlajić, S., Nikolić, Z.,& Gvozdanović-Varga, J.. (2018). Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 55(3), 125-129.
https://doi.org/10.5937/RatPov1803125I

Ignjatov M, Milosević D, Ivanović Ž, Karaman M, Vlajić S, Nikolić Z, Gvozdanović-Varga J. Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo. 2018;55(3):125-129.
doi:10.5937/RatPov1803125I .

Ignjatov, Maja, Milosević, Dragana, Ivanović, Žarko, Karaman, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia" in Ratarstvo i povrtarstvo, 55, no. 3 (2018):125-129,
https://doi.org/10.5937/RatPov1803125I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Marinković, Jelena
AU  - Ivanović, Žarko
AU  - Gvozdanović-Varga, Jelica
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/994
AB  - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
EP  - 277
SP  - 271
VL  - 133
DO  - 10.2298/ZMSPN1733271I
ER  - 

@article{
author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica",
year = "2017",
abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia",
pages = "277-271",
volume = "133",
doi = "10.2298/ZMSPN1733271I"
}

Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 133, 271-277.
https://doi.org/10.2298/ZMSPN1733271I

Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277.
doi:10.2298/ZMSPN1733271I .

Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277,
https://doi.org/10.2298/ZMSPN1733271I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović Milovanović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1026
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
EP  - 17
SP  - 9
VL  - 132
DO  - 10.2298/ZMSPN1732009I
DO  - 0352-4906
ER  - 

@article{
author = "Ignjatov, Maja and Popović Milovanović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)",
pages = "17-9",
volume = "132",
doi = "10.2298/ZMSPN1732009I, 0352-4906"
}

Ignjatov, M., Popović Milovanović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 132, 9-17.
https://doi.org/10.2298/ZMSPN1732009I

Ignjatov M, Popović Milovanović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;132:9-17.
doi:10.2298/ZMSPN1732009I .

Ignjatov, Maja, Popović Milovanović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/504
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45"
}

Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.

Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596..

Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596.

TY  - JOUR
AU  - Vučurović, Ivan
AU  - Vučurović, Ana
AU  - Nikolić, Dušan
AU  - Bulajić, Aleksandra
AU  - Milošević, D.
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/949
AB  - Leek yellow stripe virus (LYSV), one of the most important and widespread viruses of leek and garlic worldwide, is endemic in various countries of the Mediterranean basin (Katis et al. 2012). During an October 2013 survey for the presence of Allium viruses in Serbia, commercially grown leek (Allium porrum) plants with virus-like symptoms were observed in Padinska Skela (City of Belgrade District). Initially, the leaf symptoms included irregular chlorotic to light yellow dashes, particularly on the bases of leaves. The lesions later enlarged and coalesced, resulting in large, yellow stripes and the infected leaves turned yellow and flaccid, followed by die-back. Disease incidence in the leek field was estimated at 20%. A total of 15 symptomatic plants were sampled and tested by double-antibody sandwich (DAS)-ELISA test using commercial polyclonal antisera (Bioreba AG, Reinach, Switzerland) for the most important Allium viruses: LYSV, Garlic common latent virus, Onion yellow dwarf virus, and Iris yellow spot virus (Pappu et al. 2005, Katis et al. 2012). Commercial positive and negative controls and extracts from healthy leek leaves were included in each ELISA. All 15 tested leek samples were positive for LYSV and negative for the rest of tested viruses. Five carborundum-dusted plants of each Chenopodium quinoa and A. porrum ‘Varna’ were mechanically inoculated with sap prepared from ELISA-positive sample (277-13) using 0.01 M phosphate buffer (pH 7). Chlorotic local lesions on C. quinoa and streak mosaic on A. porrum ‘Varna’ were observed 5 and 16 days postinoculation, respectively, on all inoculated plants. Serological results were verified with reverse transcription (RT)-PCR. Total RNAs from all naturally and mechanically infected leek plants were extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). RT-PCR was performed using One-Step RT-PCR Kit (Qiagen) and specific primer pair 1LYSV and 2LYSV (Fajardo et al. 2001). An approximately 1000-bp fragment corresponding to the part of nuclear inclusion B (NIb) and coat protein (CP) coding region was obtained from all 20 naturally and mechanically infected leek plants, while no amplicon was recorded in the healthy and water controls. RT-PCR product obtained from one selected isolate (277-13) was purified using QIAquick PCR Purification Kit (Qiagen), sequenced directly in both directions using the same primers as for amplification, and submitted to the GenBank (Accession No. KR075504). Sequence analysis, conducted by MEGA5 software (Tamura et al. 2011), revealed that the leek isolate from Serbia showed the highest nucleotide identity of 94.8% (94.6% amino acid identity) with the sequence of LYSV isolate from leek (X89711). To our knowledge, this is the first report of natural infection of leek with LYSV in Serbia. Leek is an important and traditionally grown vegetable crop in Serbia and the presence of LYSV could cause considerable damage and severe yield losses, resulting in significant economic impact on leek production.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Leek yellow stripe virus in Leek in Serbia
EP  - 231
IS  - 1
SP  - 230
VL  - 100
DO  - 10.1094/PDIS-04-15-0432-PDN
ER  - 

@article{
author = "Vučurović, Ivan and Vučurović, Ana and Nikolić, Dušan and Bulajić, Aleksandra and Milošević, D. and Krstić, Branka and Stanković, Ivana",
year = "2016",
abstract = "Leek yellow stripe virus (LYSV), one of the most important and widespread viruses of leek and garlic worldwide, is endemic in various countries of the Mediterranean basin (Katis et al. 2012). During an October 2013 survey for the presence of Allium viruses in Serbia, commercially grown leek (Allium porrum) plants with virus-like symptoms were observed in Padinska Skela (City of Belgrade District). Initially, the leaf symptoms included irregular chlorotic to light yellow dashes, particularly on the bases of leaves. The lesions later enlarged and coalesced, resulting in large, yellow stripes and the infected leaves turned yellow and flaccid, followed by die-back. Disease incidence in the leek field was estimated at 20%. A total of 15 symptomatic plants were sampled and tested by double-antibody sandwich (DAS)-ELISA test using commercial polyclonal antisera (Bioreba AG, Reinach, Switzerland) for the most important Allium viruses: LYSV, Garlic common latent virus, Onion yellow dwarf virus, and Iris yellow spot virus (Pappu et al. 2005, Katis et al. 2012). Commercial positive and negative controls and extracts from healthy leek leaves were included in each ELISA. All 15 tested leek samples were positive for LYSV and negative for the rest of tested viruses. Five carborundum-dusted plants of each Chenopodium quinoa and A. porrum ‘Varna’ were mechanically inoculated with sap prepared from ELISA-positive sample (277-13) using 0.01 M phosphate buffer (pH 7). Chlorotic local lesions on C. quinoa and streak mosaic on A. porrum ‘Varna’ were observed 5 and 16 days postinoculation, respectively, on all inoculated plants. Serological results were verified with reverse transcription (RT)-PCR. Total RNAs from all naturally and mechanically infected leek plants were extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). RT-PCR was performed using One-Step RT-PCR Kit (Qiagen) and specific primer pair 1LYSV and 2LYSV (Fajardo et al. 2001). An approximately 1000-bp fragment corresponding to the part of nuclear inclusion B (NIb) and coat protein (CP) coding region was obtained from all 20 naturally and mechanically infected leek plants, while no amplicon was recorded in the healthy and water controls. RT-PCR product obtained from one selected isolate (277-13) was purified using QIAquick PCR Purification Kit (Qiagen), sequenced directly in both directions using the same primers as for amplification, and submitted to the GenBank (Accession No. KR075504). Sequence analysis, conducted by MEGA5 software (Tamura et al. 2011), revealed that the leek isolate from Serbia showed the highest nucleotide identity of 94.8% (94.6% amino acid identity) with the sequence of LYSV isolate from leek (X89711). To our knowledge, this is the first report of natural infection of leek with LYSV in Serbia. Leek is an important and traditionally grown vegetable crop in Serbia and the presence of LYSV could cause considerable damage and severe yield losses, resulting in significant economic impact on leek production.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Leek yellow stripe virus in Leek in Serbia",
pages = "231-230",
number = "1",
volume = "100",
doi = "10.1094/PDIS-04-15-0432-PDN"
}

Vučurović, I., Vučurović, A., Nikolić, D., Bulajić, A., Milošević, D., Krstić, B.,& Stanković, I.. (2016). First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease
American Phytopathological Society., 100(1), 230-231.
https://doi.org/10.1094/PDIS-04-15-0432-PDN

Vučurović I, Vučurović A, Nikolić D, Bulajić A, Milošević D, Krstić B, Stanković I. First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease. 2016;100(1):230-231.
doi:10.1094/PDIS-04-15-0432-PDN .

Vučurović, Ivan, Vučurović, Ana, Nikolić, Dušan, Bulajić, Aleksandra, Milošević, D., Krstić, Branka, Stanković, Ivana, "First Report of Leek yellow stripe virus in Leek in Serbia" in Plant Disease, 100, no. 1 (2016):230-231,
https://doi.org/10.1094/PDIS-04-15-0432-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/460
AB  - During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
AB  - Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
T1  - Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji
EP  - 45
IS  - 2
SP  - 42
VL  - 53
DO  - 10.5937/ratpov53-9984
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Vasić, Mirjana and Nikolić, Zorica and Tamindžić, Gordana and Ivanović, Žarko",
year = "2016",
abstract = "During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot., Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia, Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji",
pages = "45-42",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9984"
}

Ignjatov, M., Popović, T., Milošević, D., Vasić, M., Nikolić, Z., Tamindžić, G.,& Ivanović, Ž.. (2016). Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 53(2), 42-45.
https://doi.org/10.5937/ratpov53-9984

Ignjatov M, Popović T, Milošević D, Vasić M, Nikolić Z, Tamindžić G, Ivanović Ž. Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo. 2016;53(2):42-45.
doi:10.5937/ratpov53-9984 .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Vasić, Mirjana, Nikolić, Zorica, Tamindžić, Gordana, Ivanović, Žarko, "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia" in Ratarstvo i povrtarstvo, 53, no. 2 (2016):42-45,
https://doi.org/10.5937/ratpov53-9984 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/241
AB  - Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri.
AB  - Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Isolation of bacteriophages and their use in Xanthomonas spp. differantiation
T1  - Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.
EP  - 75
IS  - 2
SP  - 62
VL  - 63
ER  - 

@article{
author = "Gašić, Katarina and Ivanović, Milan and Prokić, Anđelka and Kuzmanović, Nemanja and Ignjatov, Maja and Obradović, Aleksa",
year = "2012",
abstract = "Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri., Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation, Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.",
pages = "75-62",
number = "2",
volume = "63"
}

Gašić, K., Ivanović, M., Prokić, A., Kuzmanović, N., Ignjatov, M.,& Obradović, A.. (2012). Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(2), 62-75.

Gašić K, Ivanović M, Prokić A, Kuzmanović N, Ignjatov M, Obradović A. Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja. 2012;63(2):62-75..

Gašić, Katarina, Ivanović, Milan, Prokić, Anđelka, Kuzmanović, Nemanja, Ignjatov, Maja, Obradović, Aleksa, "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation" in Zaštita bilja, 63, no. 2 (2012):62-75.

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Trkulja, Nenad
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/230
AB  - Bean production is threaten by phytopathogenic bacteria causing agents of blights, Xanthomonas axonopodis pv. phaseoli (Xap) and Pseudomonas savastanoi pv. phaseolicola (Psp). Since there is no satisfactory chemical control for the disease, the recommended measures are preventive and include use of healthy seed, crop rotation, deep plowing and use of resistant cultivars. In this work we involved a detection method for isolation of Xap and Psp from bean seed to semi-selective medium Milk Agar Tween (MT). On this medium, Xap formed yellow, mucoid and convex colonies with two hydrolysis zones (less milk and more enlightened), and Psp formed whitish-cream, flat and round colonies. The identification of Xap and Psp was confirmed using the ELISA and PCR. Due to its selectivity, easy preparation and possibility of simultaneous detection of bacteria Xap and Psp, MT medium can be recommended for routine test of seed health for local seed or seed from import.
AB  - Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium
T1  - Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge
EP  - 38
IS  - 1
SP  - 34
VL  - 49
DO  - 10.5937/ratpov49-1126
ER  - 

@article{
author = "Popović, Tatjana and Ignjatov, Maja and Jošić, Dragana and Starović, Mira and Živković, Svetlana and Aleksić, Goran and Trkulja, Nenad",
year = "2012",
abstract = "Bean production is threaten by phytopathogenic bacteria causing agents of blights, Xanthomonas axonopodis pv. phaseoli (Xap) and Pseudomonas savastanoi pv. phaseolicola (Psp). Since there is no satisfactory chemical control for the disease, the recommended measures are preventive and include use of healthy seed, crop rotation, deep plowing and use of resistant cultivars. In this work we involved a detection method for isolation of Xap and Psp from bean seed to semi-selective medium Milk Agar Tween (MT). On this medium, Xap formed yellow, mucoid and convex colonies with two hydrolysis zones (less milk and more enlightened), and Psp formed whitish-cream, flat and round colonies. The identification of Xap and Psp was confirmed using the ELISA and PCR. Due to its selectivity, easy preparation and possibility of simultaneous detection of bacteria Xap and Psp, MT medium can be recommended for routine test of seed health for local seed or seed from import., Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium, Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge",
pages = "38-34",
number = "1",
volume = "49",
doi = "10.5937/ratpov49-1126"
}

Popović, T., Ignjatov, M., Jošić, D., Starović, M., Živković, S., Aleksić, G.,& Trkulja, N.. (2012). Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 49(1), 34-38.
https://doi.org/10.5937/ratpov49-1126

Popović T, Ignjatov M, Jošić D, Starović M, Živković S, Aleksić G, Trkulja N. Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium. in Ratarstvo i povrtarstvo. 2012;49(1):34-38.
doi:10.5937/ratpov49-1126 .

Popović, Tatjana, Ignjatov, Maja, Jošić, Dragana, Starović, Mira, Živković, Svetlana, Aleksić, Goran, Trkulja, Nenad, "Detection of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastanoi pv. phaseolicola on bean seed using a Milk-tween medium" in Ratarstvo i povrtarstvo, 49, no. 1 (2012):34-38,
https://doi.org/10.5937/ratpov49-1126 . .